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Induction and quantification of gammma-H2AX foci following cx- and gamma-irradiaton

Following DNA damage the histone H2AX becomes phosphorylated and can be visualised by immunofluorescence as an indicator of DSBs in individual cells. Using a wild type hamster cell line (V79-4) exposed to either a-particles or to Co-60 gamma-rays to induce DNA DSBs at different doses (20-200OmGy), the dose dependent induction of gamma-H2AX foci were scored both manually (by eye) and using image analysis. A linearly dependence on dose was found for both radiations. The number of DSBs determined by image analysis after a post-irradiation period of 30 minutes at 37 C, is 16.6 foci/cell/Gy for alpha-irradiation and 12.2 foci/cell/Gy for gamma-irradiation; the latter being 3-4 times the levels observed by eye and comparable to gamma-radiation-induced levels of prompt DSBs more recently reported using pulse field gel electrophoresis (approx. 16 DSBs/Gy). The average size of the gamma-H2AX foci induced by alpha-irradiation (0.30 square micrometers) is approximately 1.5 times larger than those induced by gamma-irradiation (0.19 square micrometers). The timescale of induction and removal of DSBs up to 24 hours post-irradiation, was investigated with gamma-H2AX foci levels found to remain significantly higher than controls for 4 or 6 hours in gamma-irradiated samples or alpha irradiated samples, respectively. These results demonstrate that not only gamma radiation but also alpha-radiation induce phosphorylation of the H2AX histone in response to DSBs even at low doses (20mGy for gamma-rays, 1 track/cell on average for alpha-particles) and the variation in size and dephosphorylation of the induced foci is dependent on radiation quality (LET).

Leatherbarrow, E. L.↗

Interplanetary boundary layers at 1 AU

The structure and nature of discontinuities in the interplanetary magnetic field at 1 AU in the period March 18, 1971 to April 9, 1971, is determined by using high-resolution magnetic field measurements from Explorer 34. The discontinuities that were selected for this analysis occurred under a variety of interplanetary conditions at an average rate of 0.5/hr. This set does not include all discontinuities that were present, but the sample is large and it is probably representative. Both tangential and rotational discontinuities were identified, the ratio of TD's to RD's being approximately 3 to 1. Tangential discontinuities were observed every day, even among Alfvenic fluctuations. The structure of most of the boundary layers was simple and ordered, i.e., the magnetic field usually changed smoothly and monotonically from one side of the boundary layer to the other.

Burlaga, L. F.↗

Correlation function study for sea ice

For active and passive microwave remote sensing of sea ice, a correlation function of exponential form is extracted from the photograph of a horizontal thin section taken from a sample of artificially grown saline ice that closely resembled Arctic congelation sea ice. It is found that the extracted correlation lengths are consistent with the published average size of brine pockets. With the application of strong fluctuation theory and the bilocal approximation, the effective permittivity tensor is derived in the low-frequency limit for an unbounded uniaxial random medium with two-phase mixtures. Using the extracted correlation lengths, the effective permittivity tensor is computed as a function of fractional volume of brine inclusions and compared with in situ measurements at 4.8 and 9.5 GHz.

Lin, F. C.↗

Biochemical and hematologic changes after short-term space flight

Clinical laboratory data from blood samples obtained from astronauts before and after 28 flights (average duration = 6 days) of the Space Shuttle were analyzed by the paired t-test and the Wilcoxon signed-rank test and compared with data from the Skylab flights (duration approximately 28, 59, and 84 days). Angiotensin I and aldosterone were elevated immediately after short-term space flights, but the response of angiotensin I was delayed after Skylab flights. Serum calcium was not elevated after Shuttle flights, but magnesium and uric acid decreased after both Shuttle and Skylab. Creatine phosphokinase in serum was reduced after Shuttle but not Skylab flights, probably because exercises to prevent deconditioning were not performed on the Shuttle. Total cholesterol was unchanged after Shuttle flights, but low density lipoprotein cholesterol increased and high density lipoprotein cholesterol decreased. The concentration of red blood cells was elevated after Shuttle flights and reduced after Skylab flights. Reticulocyte count was decreased after both short- and long-term flights, indicating that a reduction in red blood cell mass is probably more closely related to suppression of red cell production than to an increase in destruction of erythrocytes. Serum ferritin and number of platelets were also elevated after Shuttle flights. In determining the reasons for postflight differences between the shorter and longer flights, it is important to consider not only duration but also countermeasures, differences between spacecraft, and procedures for landing and egress.

NASA Program Flight↗

Typical and Unusual Properties of Magnetic Clouds during the WIND Era

A list of 33 magnetic clouds as identified in WIND magnetic field and plasma data has been compiled. The intervals for these events are provided as part of NASA/GSFC, WIND-MFI's Website under the URL http://lepmfi.qsfc.nasa.gov/mfi/mag_cloud publ.html#table The period covered in this study is from early 1995 to November 1998 which primarily occurs in the quiet part of the solar cycle. A force free, cylindrically symmetric, magnetic field model has been applied to the field data in 1-hour averaged form for all of these events (except one small event where 10 min avg's were used) and the resulting fit-parameters examined. Each event was provided a semi-quantitatively determined quality factor (excellent, good or poor). A set of 28 good or better cases, spanning a surprisingly large range of values for its various properties, was used for further analysis. These properties are, for example, durations, attitudes, sizes, asymmetries, axial field strengths, speeds, and relative impact parameters. They will be displayed and analyzed, along with some related derived quantities, with emphasis on typical vs unusual properties and on the magnetic fields magnetic clouds' relationships to the Sun and to upstream interplanetary shocks, where possible. For example, it is remarkable how narrowly distributed the speeds of these clouds are, and the overall average speed (390 techniques km/s) is less than that normally quoted for the average solar wind speed (420 km/s) despite the fact that many of these clouds are d"drivers" of interplanetary shocks. On average, a cloud appears to be a little less symmetric when the spacecraft is able to pass close to the cloud's axis as compared to a farther out passage. The average longitude and latitude (in GSE) of the axes of the clouds are 85 degrees and 8 degrees, respectively, with standard deviations near 40 degrees. Also, the half=yearly averaged axial magnetic flux has approximately tripled. almost monotonically, from about 6 to 17 X 10(exp 29) Mx over the first 3.5 years of consideration, but with a large uncertainty on each of the half-year estimates, because of small sampling. If true,this finding implies an approximate tripling of the events' solar fluxes over this period as it goes into solar maximum.

Lepping, R. P.↗

Highly ionized gas in the Galactic disk: The l = 295 deg, b,= 0 deg interarm direction

I present new high-resolution measurements of ultraviolet interstellar absorption lines toward three distant stars in the Galactic disk in the general direction l = 295 deg, b = 0 deg. The high ionization lines of Si IV and C IV toward HD 100276, HD 103779, and HD 104705 show substantial variations on angular scales of less than 1 deg, or about 50-100 pc at the distance of the stars. The Si IV and C IV midplane densities derived for HD 100276 and HD 103779 are N(sub o) (Si IV) approximately = 1.3 x 10(exp -9) cm(exp -3) and n(sub o)(C IV) approximately = 4.5 x 10(exp -9) cvm(exp -3), approximately a factor of 2-3 lower than the Galactic averages. Toward HD 104705, the Si IV midplane density is near the average value, but the C IV midplane density is a factor of 3 higher than average. The integrated C IV and Si IV ratios along the HD 100276 and HD 100379 sight lines are similar to the N(C IV)/N(Si IV) = 3.6 +/- 1.3 ratio for a sample of halo stars. For the HD 104705 sight line, N(C IV)/N(Si IV) = 11 and N(C IV)/N(N V) greater than 10. Turbulent mixing layers provide a possible source for the observed absorption along the three sight lines as does gas associated with cooling flows. If mixing layers are the primary source of the absorption along the disk sight lines, then the entrainment velocity of the hot gas is approximately 100 km/s and the post mixed gas temperature is approximately 2 x 10(exp 5) K; same differences may exist for these mixing layers compared to those inferred for gas toward the Galactic poles. Current models of cooling, hot gas in supernova remnants, and magnetized conduction fronts do not accurately describe the high ion properties toward the disk stars in this study.

Sembach, Kenneth R.↗

Compound Specific Hydrogen Isotope Composition of Type II and III Kerogen Extracted by Pyrolysis-GC-MS-IRMS

The use of Hydrogen (H) isotopes in understanding oil and gas resource plays is in its infancy. Described here is a technique for H isotope analysis of organic compounds pyrolyzed from oil and gas shale-derived kerogen. Application of this technique will progress our understanding. This work complements that of Pernia et al. (2013, this meeting) by providing a novel method for the H isotope analysis of specific compounds in the characterization of kerogen extracted by analytically diverse techniques. Hydrogen isotope analyses were carried out entirely "on-line" utilizing a CDS 5000 Pyroprobe connected to a Thermo Trace GC Ultra interfaced with a Thermo MAT 253 IRMS. Also, a split of GC-separated products was sent to a DSQ II quadrupole MS to make semi-quantitative compositional measurements of the extracted compounds. Kerogen samples from five different basins (type II and III) were dehydrated (heated to 80 C overnight in vacuum) and analyzed for their H isotope compositions by Pyrolysis-GC-MS-TC-IRMS. This technique takes pyrolysis products separated via GC and reacts them in a high temperature conversion furnace (1450 C) which quantitatively forms H2, following a modified method of Burgoyne and Hayes, (1998, Anal. Chem., 70, 5136-5141). Samples ranging from approximately 0.5 to 1.0mg in size, were pyrolyzed at 800 C for 30s. Compounds were separated on a Poraplot Q GC column. Hydrogen isotope data from all kerogen samples typically show enrichment in D from low to high molecular weight compounds. Water (H2O) average deltaD = -215.2 (V-SMOW), ranging from -271.8 for the Marcellus Shale to -51.9 for the Polish Shale. Higher molecular weight compounds like toluene (C7H8) have an average deltaD of -89.7 0/00, ranging from -156.0 for the Barnett Shale to -50.0 for the Monterey Shale. We interpret these data as representative of potential H isotope exchange between hydrocarbons and sediment pore water during formation within each basin. Since hydrocarbon H isotopes readily exchange with water, these data may provide some useful information on gas-water or oil-water interaction in resource plays, and further as a possible indicator of paleo-environmental conditions. Alternatively, our data may be an indication of H isotope exchange with water and/or acid during the kerogen isolation process. Either of these interpretations will prove useful when deciphering H isotope data derived from kerogen analysis. More experiments are planned to discern these two or other possible scenarios.

Socki, Richard A.↗

Constraints on the Composition and Evolution of the Lunar Crust from Meteorite NWA 3163

The lunar meteorite NWA 3163 (paired with NWA 4881, 4483) is a ferroan, feldspathic granulitic breccia characterized by pigeonite, augite, olivine, maskelynite and accessory Tichromite, ilmenite and troilite. Bulk rock geochemical signatures indicate the lack of a KREEP- derived component (Eu/Eu* = 3.47), consistent with previously studied lunar granulites and anorthosites. Bulk rock chondrite-normalized signatures are however distinct from the anorthosites and granulites sampled by Apollo missions and are relatively REE-depleted. In-situ analyses of maskelynite reveal little variation in anorthite content (average An% is 96.9 +/- 1.6, 2 sigma). Olivine is relatively ferroan and exhibits very little variation in forsterite content with mean Fo% of 57.7 +/- 2.0 (2 sigma). The majority of pyroxene is low-Ca pigeonite (En57Fs33Wo10). Augite (En46Fs21Wo33) is less common, comprising approximately 10% of analyzed pyroxene. Two pyroxene thermometry on co-existing orthopyroxene and augite yield an equilibrium temperature of 1070C which is in reasonable agreement with temperatures of 1096C estimated from pigeonite compositions. Rb-Sr isotopic systematics of separated fractions yield an average measured Sr-87/Sr-87 of 0.699282+/-0.000007 (2 sigma). Sr model ages are calculated using a modern day Sr-87/Sr-86 Basaltic Achondrite Best Initial (BABI) value of 0.70475, from an initial BABI value Sr-87/Sr-86 of 0.69891 and a corresponding Rb-87/Sr-97 of 0.08716. The Sr model Thermomechanical analysis (TMA) age, which represents the time of separation of a melt from a source reservoir having chondritic evolution, is 4.56+/-0.1 Ga. A Sr model T(sub RD) age, which is a Rb depletion age and assumes no contribution from Rb in the sample in the calculation, yields 4.34+/-0.1 Ga (i.e. a minimum age). The Ar-Ar dating of paired meteorite NWA 4881 reveals an age of c. 2 Ga, likely representing the last thermal event this meteorite experienced. An older Ar-40/Ar-39 age of c. 3.5 Ga may record the thermal event which produced the granulitic texture. Additional chronological constraints will be provided by Sm-Nd systematics. Ferroan Anorthosites like NWA 3163 have been interpreted to represent direct lunar magma ocean (LMO) crystallization products. If this is the case, trace element concentrations in NWA 3163 primary mineral phases should be in equilibrium with residual LMO liquids present during crystallization of those phases. Results from petrogenetic modeling suggest that the NWA 3163 protolith did not form from crystallization of an initially LREE depleted LMO but rather require an initially chondritic LMO with early garnet crystallization. Furthermore, a two-stage crystallization model where plagioclase crystalized prior to pyroxene (93% vs. 99.5% of LMO crystallization) is implied.

McLeod, C. L.↗

A method for determining the weak statistical stationarity of a random process

A method for determining the weak statistical stationarity of a random process is presented. The core of this testing procedure consists of generating an equivalent ensemble which approximates a true ensemble. Formation of an equivalent ensemble is accomplished through segmenting a sufficiently long time history of a random process into equal, finite, and statistically independent sample records. The weak statistical stationarity is ascertained based on the time invariance of the equivalent-ensemble averages. Comparison of these averages with their corresponding time averages over a single sample record leads to a heuristic estimate of the ergodicity of a random process. Specific variance tests are introduced for evaluating the statistical independence of the sample records, the time invariance of the equivalent-ensemble autocorrelations, and the ergodicity. Examination and substantiation of these procedures were conducted utilizing turbulent velocity signals.

Sadeh, W. Z.↗

The Cl isotope composition and halogen contents of Apollo-return samples

Lunar mare basalts are depleted in F and Cl by approximately an order of magnitude relative to mid-ocean ridge basalts and contain two Cl-bearing components with elevated isotopic compositions relative to the bulk-Earth value of ∼0‰. The first is a watersoluble chloride constituting 65 ± 10% of total Cl with δ37Cl values averaging 3.0 ± 4.3‰. The second is structurally bound chloride with δ37Cl values averaging 7.3 ± 3.5‰. These high and distinctly different isotopic values are inconsistent with equilibrium fractionation processes and instead suggest early and extensive degassing of an isotopically light vapor. No relationship is observed between F/Cl ratios and δ37Cl values, which suggests that lunar halogen depletion largely resulted from the Moon-forming Giant Impact. The δ37Cl values of apatite are generally higher than the structurally bound Cl, and ubiquitously higher than the calculated bulk δ37Cl values of 4.1 ± 4.0‰. The apatite grains are not representative of the bulk rock, and instead record localized degassing during the final stages of lunar magma ocean (LMO) or later melt crystallization. The large variability in the δ37Cl values of apatite within individual thin sections further supports this conclusion. While urKREEP (primeval KREEP [potassium/rare-earth elements/phosphorus]) has been proposed to be the source of the Moon’s high Cl isotope values, the ferroan anorthosites (FANs) have the highest δ37Cl values and have a positive correlation with Cl content, and yet do not contain apatite, nor evidence of a KREEP component. The high δ37Cl values in this lithology are explained by the incorporation of a >30‰ HCl vapor from a highly evolved LMO.

The Moon↗

The variation of galactic interstellar extinction in the ultraviolet

It has been found that the wavelength dependence of interstellar extinction is not the same for all stars in the Galaxy. The present investigation is concerned with a further demonstration of the degree of variation of interstellar extinction in the UV, taking into account new extinction measurements toward 29 early-type stars. The stars do not represent a random sample. Approximately 15 of them were chosen because they have either an unusually large or an unusually small ratio of the strength of the diffuse interstellar band at 4430 A to the color excess E(B-V). Five stars have a ratio of these quantities near average and nine additional stars are illuminating reflection nebulae. Attention is given to the deviation from the average galactic extinction law, dense versus diffuse cloud environments, and the galactic longitude dependence.

Witt, A. N.↗

The distribution in lunar soil of hydrogen released by pyrolysis

The hydrogen contents of various Apollo 15 rocks and Apollo 15 and 16 soil particle types and sieve fractions have been determined by the pyrolysis method. The Apollo 15 crystalline rocks analyzed release very little hydrogen (approximately 6 micromoles H/g sample). The mineral, glass, and light-colored breccia fragments in the soils contain approximately 5, 30, and 10 micromoles H/g sample, respectively. The dark breccias and glassy agglutinates examined have much higher hydrogen contents (60 and 110 micromoles H/g sample). For soil particle diameters less than 105 microns, hydrogen content increases linearly with particle surface area per particle mass. The calculated grain surface average concentrations of hydrogen in the Apollo 15 and 16 soils fall short of experimentally determined saturation values by a factor of 20. The volume-correlated component of hydrogen in a soil increases with the maturity of the soil.

Desmarais, D. J.↗

Ultraviolet observations of M32, a cosmological comparison

The average spectral energy distribution for a sample of 3200 A. Spectral synthesis indicates that elliptical galaxies are most likely older than 9 Gyrs. The ultraviolet flux is consistent with a population of red horizontal branch stars, as those present in metal-rich globular clusters. Data for distant (Z approximately 1) first ranked cluster galaxies show indications of spectral evolution.

Spinrad, H.↗

The average X-ray/gamma-ray spectra of Seyfert galaxies from Ginga and OSSE and the origin of the cosmic X-ray background

We have obtained the first average 2-500 keV spectra of Seyfert galaxies, using the data from Ginga and Compton Gamma-Ray Observatory's (CGRO) Oriented Scintillation Spectrometer Experiment (OSSE). Our sample contains three classes of objects with markedly different spectra: radio-quiet Seyfert 1's and 2's, and radio-loud Seyfert 1's. The average radio-quiet Seyfert 1 spectrum is well-fitted by a power law continuum with the energy spectral index alpha approximately equals 0.9, a Compton reflection component corresponding to a approximately 2 pi covering solid angle, and ionized absorption. There is a high-energy cutoff in the incident power law continuum: the e-folding energy is E(sub c) approximately equals 0.6(sup +0.8 sub -0.3) MeV. The simplest model that describes this spectrum is Comptonization in a relativistic optically-thin thermal corona above the surface of an accretion disk. Radio-quiet Seyfert 2's show strong netural absorption, and there is an indication that their X-ray power laws are intrinsically harder. Finally, the radio-loud Seyfert spectrum has alpha approximately equals 0.7, moderate neutral absorption E(sub C) = 0.4(sup +0.7 sub -0.2) MeV, and no or little Compton reflection. This is incompatible with the radio-quiet Seyfert 1 spectrum, and probably indicating that the X-rays are beamed away from the accretion disk in these objects. The average spectra of Seyferts integrated over redshift with a power-law evolution can explain the hard X-ray spectrum of the cosmic background.

Zdziarski, Andrzej A.↗

The Auto-Gopher: A Wireline Rotary-Percussive Deep Sampler

Accessing regions on planetary bodies that potentially preserved biosignatures or are presently habitable is vital to meeting NASA solar system "Search for Life" exploration objectives. To address these objectives, a wireline deep rotary-percussive corer called Auto-Gopher was developed. The percussive action provides effective material fracturing and the rotation provides effective cuttings removal. To increase the drill's penetration rate, the percussive and rotary motions are operated simultaneously. Initially, the corer was designed as a percussive mechanism for sampling ice and was demonstrated in 2005 in Antarctica reaching about 2 m deep. The lessons learned suggested the need to use a combination of rotation and hammering to maximize the penetration rate. This lesson was implemented into the Auto-Gopher-I deep drill which was demonstrated to reach 3-meter deep in gypsum. The average drilling power that was used has been in the range of 100-150 Watt, while the penetration rate was approximately 2.4 m/hr. Recently, a task has started with the goal to develop Auto-Gopher-II that is equipped to execute all the necessary functions in a single drilling unit. These functions also include core breaking, retention and ejection in addition drilling. In this manuscript, the Auto-Gopher-II, its predecessors and their capability are described and discussed.

drilling↗

Formation of Minor Phases in a Nickel-Based Disk Superalloy

The minor phases of powder metallurgy disk superalloy LSHR were studied. Samples were consistently heat treated at three different temperatures for long times to approximate equilibrium. Additional heat treatments were also performed for shorter times, to then assess non-equilibrium conditions. Minor phases including MC carbides, M23C6 carbides, M3B2 borides, and sigma were identified. Their transformation temperatures, lattice parameters, compositions, average sizes and total area fractions were determined, and compared to estimates of an existing phase prediction software package. Parameters measured at equilibrium sometimes agreed reasonably well with software model estimates, with potential for further improvements. Results for shorter times representing non-equilibrium indicated significant potential for further extension of the software to such conditions, which are more commonly observed during heat treatments and service at high temperatures for disk applications.

Gabb, T. P.↗

Spacelab J air filter debris analysis

Filter debris from the Spacelab module SLJ of STS-49 was analyzed for microbial contamination. Debris for cabin and avionics filters was collected by Kennedy Space Center personnel on 1 Oct. 1992, approximately 5 days postflight. The concentration of microorganisms found was similar to previous Spacelab missions averaging 7.4E+4 CFU/mL for avionics filter debris and 4.5E+6 CFU/mL for the cabin filter debris. A similar diversity of bacterial types was found in the two filters. Of the 13 different bacterial types identified from the cabin and avionics samples, 6 were common to both filters. The overall analysis of these samples as compared to those of previous missions shows no significant differences.

Obenhuber, Donald C.↗

A Measurement of the Millimeter Emission and the Sunyaev-Zel'dovich Effect Associated with Low-Frequency Radio Sources

We present a statistical analysis of the millimeter-wavelength properties of 1.4 GHz-selected sources and a detection of the Sunyaev-Zel'dovich effect associated with the halos that host them. We stack data at 148, 218 and 277 GHz from the Atacama Cosmology Telescope at the positions of a large sample of radio AGN selected at 1.4 GHz. The thermal Sunyaev-Zel'dovich (SZ) effect associated with the halos that host the AGN is detected at the 5 sigma level through its spectral signature, representing a statistical detection of the SZ effect in some of the lowest mass halos (average M(sub 200) approximately equals 10(sup 13) solar mass h(sub 70)(exp −1) ) studied to date. The relation between the SZ effect and mass (based on weak lensing measurements of radio galaxies) is consistent with that measured by Planck for local bright galaxies. In the context of galaxy evolution models, this study confirms that galaxies with radio AGN also typically support hot gaseous halos. Adding Herschel observations allows us to show that the SZ signal is not significantly contaminated by dust emission. Finally, we analyze the contribution of radio sources to the angular power spectrum of the cosmic microwave background.

radio continuum↗