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DOE OSTI2020

How “hot” are hotspots: Statistically localizing the high-activity areas on soil and rhizosphere images

The topic of microbial hotspots in soil requires not only visualizing their spatial distribution and biochemical analyses, but also statistical approaches to identify these hotspots and separate them from the surrounding activities (background). We hypothesized that each hotspot type (e.g. enzyme activities in the rhizosphere, root exudation, localization of herbicide accumulation) is a result of local process driven by biotic and/or abiotic factors, and the process rates in the hotspots are much faster than those in the soil background. We further hypothesized that the background and hotspot activities in soil belong to different statistical distributions. Consequently, hotspot determination should be based on statistical separation of activities significantly higher than the background. We analyzed for the statistical distributions of grey values on three groups of published images: 1) 14 C images of carbon input by roots into the rhizosphere, 2) 14 C glyphosate accumulation in the plant, and 3) zymogram of leucine aminopeptidase activity in rooted soil. The two Gaussian distributions were fit (the first representing the background, the second the hotspots) to the distribution of grey values in the images, the parameters (means and standard deviations, SD) of the fitted distributions were calculated, and the background was removed. Thus, we identified hotspots as areas outside of the Mean+2SD image intensity (corresponding to the upper ~ 2.5% of activity, being over 97.5% of background values) and finally, visualized images of solely hotspot locations. Finally, these results were compared with previously used decisions on hotspot intensity thresholding (i.e. Top-25% and 17 standard thresholding approaches in ImageJ) and discussed the advantages of the Mean+2SD as well as Mean+3SD approaches. These advantages include: i) simple unification of the thresholding approach for several imaging methods with various principles of activity distribution, ii) identification of hotspots with various activity levels, iii) analysis of “time-specific” hotspots in temporal sequences of images. Compared with 17 standard thresholding methods, we concluded that objectively elucidating and separating the hotspots should be based on statistical distribution analysis, e.g. using the Mean+2SD or Mean+3SD approaches. Furthermore, this simple Mean+2SD approach delivered suitable results for three groups of images and so, helps to understand the processes responsible for the highest activities and elucidate hotspots.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2021

Statistical analysis of correlations in the x-ray induced Coulomb explosion of iodopyridine

Coulomb explosion imaging is a promising experimental tool to study individual molecules. Here, in this work simulation data for the x-ray induced Coulomb explosion of 2-iodopyridine is analyzed and the involved fragmentation dynamics are described. It is found that particular final ion momenta show correlations that reflect a collision of two atoms during the explosion. Variations of the proton kinetic energies can be associated with variations in the charge build-up time. Covariances of the forces during the explosion can be utilized to simplify the description of the dynamics in reduced dimensionality using only four collective coordinates.

74 ATOMIC AND MOLECULAR PHYSICS↗

NASA NTRS2009

A Biomechanical Approach to Assessing Hip Fracture Risk

Bone loss in microgravity is well documented, but it is difficult to quantify how declines in bone mineral density (BMD) contribute to an astronaut's overall risk of fracture upon return. This study uses a biomechanical approach to assessing hip fracture risk, or Factor of Risk (Phi), which is defined as the ratio of applied load to bone strength. All long-duration NASA astronauts from Expeditions 1-18 were included in this study (n=25), while crewmembers who flew twice (n=2) were treated as separate subjects. Bone strength was estimated based on an empirical relationship between areal BMD at the hip, as measured by DXA, and failure load, as determined by mechanical testing of cadaver femora. Fall load during a sideways fall was calculated from a previously developed biomechanical model, which takes into account body weight, height, gender, and soft tissue thickness overlying the lateral aspect of the hip that serves to attenuate the impact force. While no statistical analyses have been performed yet, preliminary results show that males in this population have a higher FOR than females, with a post- flight Phi of 0.87 and 0.36, respectively. FOR increases 5.1% from preflight to postflight, while only one subject crossed the fracture "threshold" of Phi = 1, for a total of 2 subjects with a postflight Phi > 1. These results suggest that men may be at greater risk for hip fracture due largely in part to their relatively thin soft tissue padding as compared to women, since soft tissue thickness has the highest correlation (R(exp 2)= .53) with FOR of all subject-specific parameters. Future work will investigate changes in FOR during recovery to see if baseline risk levels are restored upon return to 1-g activity. While dual x-ray absorptiometry (DXA) is the most commonly used clinical measure of bone health, it fails to provide compartment-specific information that is useful in assessing changes to bone quality as a result of microgravity exposure. Peripheral quantitative computed tomography (pQCT) accomplishes this by imaging transverse "slices" of the long bones. This project was a re-analysis of a 90 day bed rest study to determine if changes to cortical and trabecular compartments could be detected in the distal tibia with statistical significance using a new pQCT image analysis method. Nearly all changes in bone mineral density (BMD) and cross sectional area (CSA) measures were seen with statistical significance, with the exception of a change in cortical BMD. Total bone CSA increased by 1.1 % (p =0.01), cortical CSA decreased by - 5.6% (p<0.001) and trabecular CSA increased by 1.76% (p=0.007); the combination of which suggests bone resorption occurred at the endocortical surface in response to mechanical unloading by bed rest. Furthermore, total BMD and trabecular BMD decreased (-3.8%, p=0.001 and -2.8%, p =0.007, respectively), while decreases in cortical BMD failed to reach significance (-1.2%, p=0.07). Given that compartment-specific changes are seen with significance and are likely to influence bone strength, it is recommended that pQCT remain a standard measure used in bed rest because it provides a unique measure by which to better evaluate the efficacy of countermeasures to microgravity-induced bone loss.

Ellman, Rachel↗

NASA NTRS1991

Data simulation for the Lightning Imaging Sensor (LIS)

This project aims to build a data analysis system that will utilize existing video tape scenes of lightning as viewed from space. The resultant data will be used for the design and development of the Lightning Imaging Sensor (LIS) software and algorithm analysis. The desire for statistically significant metrics implies that a large data set needs to be analyzed. Before 1990 the quality and quantity of video was insufficient to build a usable data set. At this point in time, there is usable data from missions STS-34, STS-32, STS-31, STS-41, STS-37, and STS-39. During the summer of 1990, a manual analysis system was developed to demonstrate that the video analysis is feasible and to identify techniques to deduce information that was not directly available. Because the closed circuit television system used on the space shuttle was intended for documentary TV, the current value of the camera focal length and pointing orientation, which are needed for photoanalysis, are not included in the system data. A large effort was needed to discover ancillary data sources as well as develop indirect methods to estimate the necessary parameters. Any data system coping with full motion video faces an enormous bottleneck produced by the large data production rate and the need to move and store the digitized images. The manual system bypassed the video digitizing bottleneck by using a genlock to superimpose pixel coordinates on full motion video. Because the data set had to be obtained point by point by a human operating a computer mouse, the data output rate was small. The loan and subsequent acquisition of a Abekas digital frame store with a real time digitizer moved the bottleneck from data acquisition to a problem of data transfer and storage. The semi-automated analysis procedure was developed using existing equipment and is described. A fully automated system is described in the hope that the components may come on the market at reasonable prices in the next few years.

Boeck, William L.↗

NASA NTRS2015

Effect of Cloud Fraction on Near-Cloud Aerosol Behavior in the MODIS Atmospheric Correction Ocean Color Product

Characterizing the way satellite-based aerosol statistics change near clouds is important for better understanding both aerosol-cloud interactions and aerosol direct radiative forcing. This study focuses on the question of whether the observed near-cloud increases in aerosol optical thickness and particle size may be explained by a combination of two factors: (i) Near-cloud data coming from areas with higher cloud fractions than far-from-cloud data and (ii) Cloud fraction being correlated with aerosol optical thickness and particle size. This question is addressed through a statistical analysis of aerosol parameters included in the MODIS (MODerate resolution Imaging Spectroradiometer) ocean color product. Results from ten Septembers (2002-2011) over part of the northeast Atlantic Ocean confirm that the combination of these two factors working together explains a significant but not dominant part (in our case, 15%-30%) of mean optical thickness changes near clouds. Overall, the findings show that cloud fraction plays a large role in shaping the way aerosol statistics change with distance to clouds. This implies that both cloud fraction and distance to clouds are important to consider when aerosol-cloud interactions or aerosol direct radiative effects are examined in satellite or modeling studies.

Remote sensing↗

NASA NTRS1975

The interpretation of simultaneous soft X-ray spectroscopic and imaging observations of an active region

Simultaneous soft X-ray spectroscopic and broad-band imaging observations of an active region have been analyzed together to determine the parameters which describe the coronal plasma. From the spectroscopic data, models of temperature-emission measure-elemental abundance have been constructed which provide acceptable statistical fits. By folding these possible models through the imaging analysis, models which are not self-consistent can be rejected. In this way, only the oxygen, neon, and iron abundances of Pottasch (1967), combined with either an isothermal or exponential temperature-emission-measure model, are consistent with both sets of data. Contour maps of electron temperature and density for the active region have been constructed from the imaging data. The implications of the analysis for the determination of coronal abundances and for future satellite experiments are discussed.

Davis, J. M.↗

NASA NTRS1991

Clutter modeling of the Denver Airport and surrounding areas

To accurately simulate and evaluate an airborne Doppler radar as a wind shear detection and avoidance sensor, the ground clutter surrounding a typical airport must be quantified. To do this, an imaging airborne Synthetic Aperture Radar (SAR) was employed to investigate and map the normalized radar cross sections (NRCS) of the ground terrain surrounding the Denver Stapleton Airport during November of 1988. Images of the Stapleton ground clutter scene were obtained at a variety of aspect and elevation angles (extending to near-grazing) at both HH and VV polarizations. Presented here, in viewgraph form with commentary, are the method of data collection, the specific observations obtained of the Denver area, a summary of the quantitative analysis performed on the SAR images to date, and the statistical modeling of several of the more interesting stationary targets in the SAR database. Additionally, the accompanying moving target database, containing NRCS and velocity information, is described.

Harrah, Steven D.↗

NASA NTRS1992

Accurate Inventories Of Irrigated Land

System for taking land-use inventories overcomes two problems in estimating extent of irrigated land: only small portion of large state surveyed in given year, and aerial photographs made on 1 day out of year do not provide adequate picture of areas growing more than one crop per year. Developed for state of California as guide to controlling, protecting, conserving, and distributing water within state. Adapted to any large area in which large amounts of irrigation water needed for agriculture. Combination of satellite images, aerial photography, and ground surveys yields data for computer analysis. Analyst also consults agricultural statistics, current farm reports, weather reports, and maps. These information sources aid in interpreting patterns, colors, textures, and shapes on Landsat-images.

Wall, S.↗

NASA NTRS2005

A Statistical Analysis of Loop-Top Motion in Solar Limb Flares

Previous studies of hot, thermal solar flare loops imaged with the Ramaty High Energy Solar Spectroscopic Imager (RHESSI) have identified several flares for which the loop top shrinks downward early in the impulsive phase and then expands upward later in the impulsive phase (Sui & Holman 2003; Sui, Holman & Dennis 2004; Veronig et al. 2005). This early downward motion is not predicted by flare models. We study a statistical sample of RHESSI flares to assess how common this evolution is and to better characterize it. In a sample of 88 flares near the solar lin$ that show identifiable loop structure in RHESSI images, 66% (58 flares) showed downward loop-top motion followed by upward motion. We therefore conclude that the early downward motion is a frequent characteristic of flare loops. We obtain the distribution of the timing of the change from downward to upward motion relative to flare start and peak times. We also obtain the distributions of downward and upward speeds.

Holman, Gordon D.↗

DOE OSTI2023

Power Electronics Materials and Bonded Interfaces - Reliability and Lifetime

High temperature operation of wide bandgap devices continue to be a challenge for the power electronics packages. Thermal performance and reliability are important factors that determine the viability of a bonded interface for operation at high temperatures. In this presentation, we present the technical approach and key results from the research on sintered silver, transient liquid phase alloy, and polymeric materials. A lifetime prediction model that incorporates the thermomechanical behavior of sintered silver at 200C was developed. The copper-aluminum transient alloy completed 350 thermal cycles from -40C to 200C and little increase in the defect level was observed. In addition to material research, we initiated a time-series analysis on the scanning acoustic microscope images of eutectic solder to explore statistical forecasting methods and machine learning techniques. Initial results that report the accuracy of a few different statistical models are presented.

ADVANCED PROPULSION SYSTEMS↗

NASA NTRS1991

Environmental analysis using integrated GIS and remotely sensed data - Some research needs and priorities

This paper discusses some basic scientific issues and research needs in the joint processing of remotely sensed and GIS data for environmental analysis. Two general topics are treated in detail: (1) scale dependence of geographic data and the analysis of multiscale remotely sensed and GIS data, and (2) data transformations and information flow during data processing. The discussion of scale dependence focuses on the theory and applications of spatial autocorrelation, geostatistics, and fractals for characterizing and modeling spatial variation. Data transformations during processing are described within the larger framework of geographical analysis, encompassing sampling, cartography, remote sensing, and GIS. Development of better user interfaces between image processing, GIS, database management, and statistical software is needed to expedite research on these and other impediments to integrated analysis of remotely sensed and GIS data.

Davis, Frank W.↗

DOE OSTI2024

Batch VUV4 characterization for the SBC-LAr10 scintillating bubble chamber

The Scintillating Bubble Chamber (SBC) collaboration purchased 32 Hamamatsu VUV4 silicon photomultipliers (SiPMs) for use in SBC-LAr10, a bubble chamber containing 10 kg of liquid argon. A dark-count characterization technique, which avoids the use of a single-photon source, was used at two temperatures to measure the VUV4 SiPMs breakdown voltage (V BD ), the SiPM gain (g SiPM ), the rate of change of g SiPM with respect to voltage (m), the dark count rate (DCR), and the probability of a correlated avalanche (P CA ) as well as the temperature coefficients of these parameters. A Peltier-based chilled vacuum chamber was developed at Queen's University to cool down the Quads to 233.15 ± 0.2 K and 255.15 ± 0.2 K with average stability of ±20 mK. An analysis framework was developed to estimate V BD to tens of mV precision and DCR close to Poissonian error. The temperature dependence of V BD was found to be 56 ± 2 mV K -1 , and m on average across all Quads was found to be (459 ± 3(stat.)±23(sys.))× 10 3 e- PE -1 V -1 . The average DCR temperature coefficient was estimated to be 0.099 ± 0.008 K -1 corresponding to a reduction factor of 7 for every 20 K drop in temperature. The average temperature dependence of P CA was estimated to be 4000 ± 1000 ppm K -1 . P CA estimated from the average across all SiPMs is a better estimator than the P CA calculated from individual SiPMs, for all of the other parameters, the opposite is true. All the estimated parameters were measured to the precision required for SBC-LAr10, and the Quads will be used in conditions to optimize the signal-to-noise ratio.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

DOE OSTI2021

RGB wavefront sensor for turbulence mitigation

A compact adaptive optics system for long-range horizontal paths imaging that improves degraded images. The system uses a filter that corresponds to the three colors in a typical color detector element, one or more optic elements, a deformable mirror, and a detector. Focus errors, due to turbulence, in the image recorded by the detector element show up as image shifts in the three distinct color images. The shifts and statistics of these shifts between these simultaneous images are used to create control signals for the deformable mirror resulting in a compact adaptive optic system for horizontal paths without need for a point source located at the distance scene being imaged. Analysis of the relative pixel shifts in various regions of the image provides third order statistics revealing tip/tilt and additional Zernikes modes that are used to control a deformable mirror without the need for a guide star/point-source.

O'Neill, Mary Dominique↗

DOE OSTI2020

Soft x-ray spectrum unfold of K-edge filtered x-ray diode arrays using cubic splines

Cubic spline interpolation is able to recover temporally and spectrally resolved soft x-ray flux from an array of K-edge filtered x-ray diodes without the need for a priori assumptions about the spectrum or the geometry of the emitting volume. The mathematics of the cubic spline interpolation is discussed in detail. The analytic nature of the cubic spline solution allows for analytical error propagation, and the method of calculating the error for radiation temperature, spectral power, and confidence intervals of the unfolded spectrally resolved flux is explained. An unfold of a blackbody model demonstrates the accuracy of the cubic spline unfold. Tests of cubic spline performance using spectrally convolved detailed atomic model simulation results have been performed to measure the method's ability to conserve spectral power to within a factor of 2 or better in line-dominated regimes. The unfold is also demonstrated to work when information from the x-ray diode array is limited due to high signal-to-noise ratios or lack of signal due to over-attenuation or over-filtration of the x-ray diode signal. Here, the robustness of the unfold with respect to background subtraction and raw signal processing, signal alignment between diode traces, limited signal information, and initial conditions is discussed. Results from an example analysis of a halfraum drive are presented to demonstrate the capabilities of the unfold in comparison with previously established methods.

47 OTHER INSTRUMENTATION↗

NASA NTRS2005

CSAM Metrology Software Tool

CSAM Metrology Software Tool (CMeST) is a computer program for analysis of false-color CSAM images of plastic-encapsulated microcircuits. (CSAM signifies C-mode scanning acoustic microscopy.) The colors in the images indicate areas of delamination within the plastic packages. Heretofore, the images have been interpreted by human examiners. Hence, interpretations have not been entirely consistent and objective. CMeST processes the color information in image-data files to detect areas of delamination without incurring inconsistencies of subjective judgement. CMeST can be used to create a database of baseline images of packages acquired at given times for comparison with images of the same packages acquired at later times. Any area within an image can be selected for analysis, which can include examination of different delamination types by location. CMeST can also be used to perform statistical analyses of image data. Results of analyses are available in a spreadsheet format for further processing. The results can be exported to any data-base-processing software.

Vu, Duc↗

DOE OSTI2024

Qualification and Quantification of Porosity at the Top of the Fuel Pins in Metallic Fuels Using Image Processing

Approximately 130,000 metal fuel pins were irradiated in the Experimental Breeder Reactor II (EBR-II) during its 30 years of operation to develop and characterize existing and prospective fuels. For many of the metal fuel irradiation experiments, neutron radiography imaging was performed to characterize fuel behavior, such as fuel axial expansion. While several fuel expansion results obtained from neutron radiography imaging have been published, the analysis of neutron radiography for the purpose of describing statistical properties of porous matter formed on top of the fuel pins, also referred to as fluff in previous publications, is significantly less represented in the literature with just a single paper so far. This study aims to validate and augment results reported in previous publications using automated image processing. The paper describes the statistical properties of the porous matter in terms of nine parameters derived from radiography images and correlates those parameters with such fuel properties as composition, expansion, temperature, and burnup. The reported results are based on 1097 fuel pins of eight different fuel compositions. For three major fuel types, U-10Zr, U-8Pu-10Zr, and U-19Pu-10Zr, a clear negative correlation is found between the Pu content and five parameters describing the amount of porous matter generated. The parameters describing granularity properties, however, showed either negative correlation or nonlinear dependency from fuel composition. The parameters describing the amount showed a positive correlation with fuel axial expansion, while granularity parameters showed a negative correlation with axial expansion. The dependency on cladding temperature was found to be weak. A positive correlation is demonstrated for volume parameters and fuel burnup. In general, reported results confirm and validate findings published in previous studies using a much larger number of pins and automated processing techniques, which easily lend themselves to reproducibility, thus avoiding subjective bias.

36 MATERIALS SCIENCE↗

DOE OSTI2020

Three-dimensional super line-localization in low signal-to-noise microscope images via prior-apprised unsupervised learning (PAUL)

Biological processes such as processive enzyme turnover and intracellular cargo tracking involve the dynamic motion of a small "article" along a curvilinear biopolymer track. To understand these processes that occur across multiple length and time scales, one must acquire both the trajectory of the particle and the position of the track along which it moves, possibly by combining high-resolution single-particle tracking with conventional microscopy. Yet, usually there is a significant resolution mismatch between these modalities: while the tracked particle is localized with a precision of 10 nm, the image of the surroundings is limited by optical difraction, with 200 nm lateral and 500 nm axial resolutions. Compared to the particle's trajectory, the surrounding curvilinear structure appears as a blurred and noisy image. This disparity in the spatial resolutions of the particle trajectory and the surrounding curvilinear structure image makes data reconstruction, as well as interpretation, particularly challenging. Analysis is further complicated when the curvilinear structures are oriented arbitrarily in 3D space. Here, we present a prior-apprised unsupervised learning (PAUL) approach to extract information from 3D images where the underlying features resemble a curved line such as a filament or microtubule. This three-stage framework starts with a Hessian-based feature enhancement, which is followed by feature registration, where local line segments are detected on repetitively sampled subimage tiles. In the final stage, statistical learning, segments are clustered based on their geometric relationships. Principal curves are then approximated from each segment group via statistical tools including principal component analysis, bootstrap and kernel transformation. This procedure is characterized on simulated images, where sub-voxel medium deviations from true curves have been achieved. The 3D PAUL approach has also been implemented for successful line localization in experimental 3D images of gold nanowires obtained using a multifocal microscope. Lastly, this work not only bridges the resolution gap between two microscopy modalities, but also allows us to conduct 3D super line-localization imaging experiments, without using super-resolution techniques.

42 ENGINEERING↗

NASA NTRS1993

Statistical Approach To Determination Of Texture In SAR

Paper presents statistical approach to analysis of texture in synthetic-aperture-radar (SAR) images. Objective: to extract intrinsic spatial variability of distributed target from overall spatial variability of SAR image.

Rignot, Eric J.↗