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Remote sensing science for the Nineties; Proceedings of IGARSS '90 - 10th Annual International Geoscience and Remote Sensing Symposium, University of Maryland, College Park, May 20-24, 1990. Vols. 1, 2, & 3

Various papers on remote sensing (RS) for the nineties are presented. The general topics addressed include: subsurface methods, radar scattering, oceanography, microwave models, atmospheric correction, passive microwave systems, RS in tropical forests, moderate resolution land analysis, SAR geometry and SNR improvement, image analysis, inversion and signal processing for geoscience, surface scattering, rain measurements, sensor calibration, wind measurements, terrestrial ecology, agriculture, geometric registration, subsurface sediment geology, radar modulation mechanisms, radar ocean scattering, SAR calibration, airborne radar systems, water vapor retrieval, forest ecosystem dynamics, land analysis, multisensor data fusion. Also considered are: geologic RS, RS sensor optical measurements, RS of snow, temperature retrieval, vegetation structure, global change, artificial intelligence, SAR processing techniques, geologic RS field experiment, stochastic modeling, topography and Digital Elevation model, SAR ocean waves, spaceborne lidar and optical, sea ice field measurements, millimeter waves, advanced spectroscopy, spatial analysis and data compression, SAR polarimetry techniques. Also discussed are: plant canopy modeling, optical RS techniques, optical and IR oceanography, soil moisture, sea ice back scattering, lightning cloud measurements, spatial textural analysis, SAR systems and techniques, active microwave sensing, lidar and optical, radar scatterometry, RS of estuaries, vegetation modeling, RS systems, EOS/SAR Alaska, applications for developing countries, SAR speckle and texture.

Source record↗

Thermographic Imaging of Material Loss in Boiler Water-Wall Tubing by Application of Scanning Line Source

Localized wall thinning due to corrosion in utility boiler water-wall tubing is a significant inspection concern for boiler operators. Historically, conventional ultrasonics has been used for inspection of these tubes. This technique has proven to be very manpower and time intensive. This has resulted in a spot check approach to inspections, documenting thickness measurements over a relatively small percentage of the total boiler wall area. NASA Langley Research Center has developed a thermal NDE technique designed to image and quantitatively characterize the amount of material thinning present in steel tubing. The technique involves the movement of a thermal line source across the outer surface of the tubing followed by an infrared imager at a fixed distance behind the line source. Quantitative images of the material loss due to corrosion are reconstructed from measurements of the induced surface temperature variations. This paper will present a discussion of the development of the thermal imaging system as well as the techniques used to reconstruct images of flaws. The application of the thermal line source coupled with the analysis technique represents a significant improvement in the inspection speed for large structures such as boiler water-walls. A theoretical basis for the technique will be presented which explains the quantitative nature of the technique. Further, a dynamic calibration system will be presented for the technique that allows the extraction of thickness information from the temperature data. Additionally, the results of applying this technology to actual water-wall tubing samples and in situ inspections will be presented.

Cramer, K. Elliott↗

Estimation of Unsteady Aerodynamic Models from Dynamic Wind Tunnel Data

Demanding aerodynamic modelling requirements for military and civilian aircraft have motivated researchers to improve computational and experimental techniques and to pursue closer collaboration in these areas. Model identification and validation techniques are key components for this research. This paper presents mathematical model structures and identification techniques that have been used successfully to model more general aerodynamic behaviours in single-degree-of-freedom dynamic testing. Model parameters, characterizing aerodynamic properties, are estimated using linear and nonlinear regression methods in both time and frequency domains. Steps in identification including model structure determination, parameter estimation, and model validation, are addressed in this paper with examples using data from one-degree-of-freedom dynamic wind tunnel and water tunnel experiments. These techniques offer a methodology for expanding the utility of computational methods in application to flight dynamics, stability, and control problems. Since flight test is not always an option for early model validation, time history comparisons are commonly made between computational and experimental results and model adequacy is inferred by corroborating results. An extension is offered to this conventional approach where more general model parameter estimates and their standard errors are compared.

Murphy, Patrick↗

Remote sensing of seasonal distribution of precipitable water vapor over the oceans and the inference of boundary-layer structure

From the depth of the water vapor spectral lines in the 8-9 micron window region, measured by the Nimbus 4 Infrared Interferometer Spectrometer (IRIS) with a resolution of about 3/cm, the precipitable water vapor over the oceans is remotely sensed. In addition the IRIS spectral data in the 11-13 micron window region have been used to derive the sea surface temperature (SST). Seasonal maps of w on the oceans deduced from the spectral data reveal the dynamical influence of the large-scale atmospheric circulation. With the help of a model for the vertical distribution of water vapor, the configuration of the atmospheric boundary layer over the oceans can be inferred from these remotely sensed w and SST. The gross seasonal mean structure of the boundary layer inferred in this fashion reveals the broad areas of trade wind inversion and the convectively active areas such as the ITCZ. The derived information is in reasonable agreement with some observed climatological patterns over the oceans.

Prabhakara, C.↗

M2-SCREAM: A Stratospheric Composition Reanalysis of Aura MLS Data With MERRA-2 Transport

MERRA-2 Stratospheric Composition Reanalysis of Aura Microwave Limb Sounder (M2-SCREAM) is a new reanalysis of stratospheric ozone, water vapor, hydrogen chloride (HCl), nitric acid (HNO 3 ) and nitrous oxide (N 2 O) between 2004 and the present (with a latency of several months). The assimilated fields are provided at a 50-km horizontal resolution and at a three-hourly frequency. M2-SCREAM assimilates version 4.2 Microwave Limb Sounder (MLS) profiles of the five constituents alongside total ozone column from the Ozone Monitoring Instrument. Dynamics and tropospheric water vapor are constrained by the MERRA-2 reanalysis. The assimilated species are in excellent agreement with the MLS observations, except for HNO 3 in polar night, where data are not assimilated. Comparisons against independent observations show that the reanalysis realistically captures the spatial and temporal variability of all the assimilated constituents. In particular, the standard deviations of the differences between M2-SCREAM and constituent mixing ratio data from The Atmospheric Chemistry Experiment Fourier Transform Spectrometer are much smaller than the standard deviations of the measured constituents. Evaluation of the reanalysis against aircraft data and balloon-borne frost point hygrometers indicates a faithful representation of small-scale structures in the assimilated water vapor, HNO 3 and ozone fields near the tropopause. Comparisons with independent observations and a process-based analysis of the consistency of the assimilated constituent fields with the MERRA-2 dynamics and with large-scale stratospheric processes demonstrate the utility of M2-SCREAM for scientific studies of chemical and transport variability on time scales ranging from hours to decades. Analysis uncertainties and guidelines for data usage are provided.

MERRA-2↗

Molecular Simulations in Astrobiology

One of the main goals of astrobiology is to understand the origin of cellular life. In the absence of any record of the earliest ancestors of contemporary cells, protocells, the most direct way to test our understanding of their characteristics is to construct laboratory models of protocells. Such efforts, currently underway in the NASA Astrobiology Program, are accompanied by computational studies aimed at explaining self-organization of simple molecules into ordered structures and developing designs of molecules that are capable of performing protocellular functions. Many of these functions, such as importing nutrients, capturing and storing energy, and responding to changes in the environment, are carried out by proteins bound to membranes. We use computer simulations to address the following, questions about these proteins: (1) How do small proteins (peptides) organize themselves into ordered structures at water-membrane interfaces and insert into membranes? (2) How do peptides aggregate to form membrane-spannin(y structures (e.g., channels)? (3) By what mechanisms do such aggregates perform their functions? The simulations are performed using the molecular dynamics (MD) method. In this method, Newton's equations of motion for each atom in the system are solved iteratively. At each time step, the forces exerted on each atom by the remaining atoms are evaluated by dividing them into two parts. Short-range forces are calculated directly in real space while long-range forces are evaluated in reciprocal space, usually using a particle-mesh algorithm which is of order O(NlnN). Currently, a time step of 2 femtoseconds is typically used, thereby making studies of problems occurring on multi-nanosecond time scales (10(exp 6) - 10(exp 8) time steps) accessible. To address a broader range of problems, simulations need to be extended by three orders of magnitude. Such an extension requires both algorithmic improvements and codes scalable to a large number of parallel processors. Work in this direction is in progress. Two specific series of simulations that demonstrate how peptides self-organize and function in membranes are discussed. In one series of simulations, it was shown that nonpolar peptides, disordered in water, translocate to the nonpolar interior of the membrane and, simultaneously, fold into two different helical structures, which remain in equilibrium. Once in the membrane, the peptides can readily change their orientation, especially in response to local electric fields. This structural and orientational flexibility of peptides with changing conditions may have provided a mechanism of transmitting signals between the environment and the interior of the protocell. In another series of simulations, the mechanism by which a simple protein channel efficiently mediates proton transport across membranes was investigated. This process is a key step in cellular bioenergetics. In the channel under study, proton transport is gated by four histidines that occlude the channel pore. The simulations demonstrate that protons move through the gate by a "shuttle" mechanism, wherein one histidine is protonated on the extracellular side and, subsequently, the proton bound on the opposite side is released.

Pohorille, Andrew↗

Proton Transport

The transport of protons across membranes is an essential process for both bioenergetics of modern cells and the origins of cellular life. All living systems make use of proton gradients across cell walls to convert environmental energy into a high-energy chemical compound, adenosine triphosphate (ATP), synthesized from adenosine diphosphate. ATP, in turn, is used as a source of energy to drive many cellular reactions. The ubiquity of this process in biology suggests that even the earliest cellular systems were relying on proton gradient for harvesting environmental energy needed to support their survival and growth. In contemporary cells, proton transfer is assisted by large, complex proteins embedded in membranes. The issue addressed in this Study was: how the same process can be accomplished with the aid of similar but much simpler molecules that could have existed in the protobiological milieu? The model system used in the study contained a bilayer membrane made of phospholipid, dimyristoylphosphatidylcholine (DMPC) which is a good model of the biological membranes forming cellular boundaries. Both sides of the bilayer were surrounded by water which simulated the environment inside and outside the cell. Embedded in the membrane was a fragment of the Influenza-A M$_2$ protein and enough sodium counterions to maintain system neutrality. This protein has been shown to exhibit remarkably high rates of proton transport and, therefore, is an excellent model to study the formation of proton gradients across membranes. The Influenza M$_2$ protein is 97 amino acids in length, but a fragment 25 amino acids long. which contains a transmembrane domain of 19 amino acids flanked by three amino acids on each side. is sufficient to transport protons. Four identical protein fragments, each folded into a helix, aggregate to form small channels spanning the membrane. Protons are conducted through a narrow pore in the middle of the channel in response to applied voltage. This channel is large enough to contain water molecules. and is normally filled with water. In analogy to the mechanism of proton transfer in some other channels, it has been postulated that protons are translocated along the network of water molecules filling the pore of the channel. This mechanism, however, must involve an additional important step because the channel contains four histidine amino acid residues, one from each of the helices, which are sufficiently large to occlude the pore and interrupt the water network. The histidine residues ensure channel selectivity by blocking transport of small ions, such as sodium or potassium. They have been also implicated in gating protons due to the ability of each histidine to become positively charged by accepting an additional proton. Two mechanisms of gating have been proposed. In one mechanism, all four histidines acquire an additional proton and, due to repulsion between their positive charges, move away from one another, thus opening the channel. The alternative mechanism relies of the ability of protons to move between different atoms in a molecule (tautomerization). Thus, a proton is captured on one side of the gate while another proton is released from the opposite side, and the molecule returns to the initial state through tautomerization. The simulations were designed to test these two mechanisms. Large-scale, atomic-level molecular dynamics simulations of the channel with the histidine residues in different protonation states revealed that all intermediate states of the system involved in the tautomerization mechanism are structurally stable and the arrangement of water molecules in the channel is conducive to the proton transport. In contrast, in the four-protonated state, postulated to exist in the gate-opening mechanism, the electrostatic repulsion between the histidine residues appears to be so large that the channel loses its structural integrity and one helix moves away from the remaining three. Additional information is contained within the original extended abstract.

Pohorille, Andrew↗

On the Pathways of the Return Flow of the Meridional Overturning Circulation in the Tropical Atlantic

A numerical model of the tropical Atlantic ocean is used to investigate the upper layer pathways of the Meridional Overturning Circulation (MOC) in the tropical Atlantic. The main focus of this thesis is on those parts of the tropical circulation that are thought to be important for the MOC return flow, but whose dynamics have not been understood yet. It is shown how the particular structure of the tropical gyre and the MOO act to inhibit the flow of North Atlantic water into the equatorial thermocline. As a result, the upper layers of the tropical Atlantic are mainly fed by water from the South Atlantic. The processes that carry the South Atlantic water across the tropical Atlantic into the North Atlantic as part of the MOO are described here, and three processes that were hitherto not understood are explained as follows: The North Brazil Current rings are created as the result of the reflection of Rossby waves at the South American coast. These Rossby waves are generated by the barotropically unstable North Equatorial Countercurrent. The deep structure of the rings can be explained by merger of the wave's anticyclones with the deeper intermediate eddies that are generated as the intermediate western boundary current crosses the equator. The bands of strong zonal velocity in intermediate depths along the equator have hitherto been explained as intermediate currents. Here, an alternative interpretation of the observations is offered: The Eulerian mean flow along the equator is negligible and the observations are the signature of strong seasonal Rossby waves. The previous interpretation of the observations can then be explained as aliasing of the tropical wave field. The Tsuchyia Jets are driven by the Eliassen-Palm flux of the tropical instability waves. The equatorial current system with its strong shears is unstable and generates tropical instability waves.

Jochum, Markus↗

Structural Basis for "Flip-Flop" Action of Human Pyruvate Dehydrogenase

The derivative of vitamin B1, thiamin pyrophosphate is a cofactor of pyruvate dehydrogenase, a component enzyme of the mitochondrial pyruvate dehydrogenase multienzyme complex that plays a major role in directing energy metabolism in the cell. This cofactor is used to cleave the C(sup alpha)-C(=O) bond of pyruvate followed by reductive acetyl transfer to lipoyl-dihydrolipoamide acetyltransferase. In alpha(sub 2)beta(sub 2)-tetrameric human pyruvate dehydrogenase, there are two cofactor binding sites, each of them being a center of independently conducted, although highly coordinated enzymatic reactions. The dynamic nonequivalence of two, otherwise chemically equivalent, catalytic sites can now be understood based on the recently determined crystal structure of the holo-form of human pyruvate dehydrogenase at 1.95A resolution. The structure of pyruvate dehydrogenase was determined using a combination of MAD phasing and molecular replacement followed by rounds of torsion-angles molecular-dynamics simulated-annealing refinement. The final pyruvate dehydrogenase structure included coordinates for all protein amino acids two cofactor molecules, two magnesium and two potassium ions, and 742 water molecules. The structure was refined to R = 0.202 and R(sub free) = 0.244. Our structural analysis of the enzyme folding and domain assembly identified a simple mechanism of this protein motion required for the conduct of catalytic action.

Ciszak, Ewa↗

An Examination of the Nature of Global MODIS Cloud Regimes

We introduce global cloud regimes (previously also referred to as "weather states") derived from cloud retrievals that use measurements by the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument aboard the Aqua and Terra satellites. The regimes are obtained by applying clustering analysis on joint histograms of retrieved cloud top pressure and cloud optical thickness. By employing a compositing approach on data sets from satellites and other sources, we examine regime structural and thermodynamical characteristics. We establish that the MODIS cloud regimes tend to form in distinct dynamical and thermodynamical environments and have diverse profiles of cloud fraction and water content. When compositing radiative fluxes from the Clouds and the Earth's Radiant Energy System instrument and surface precipitation from the Global Precipitation Climatology Project, we find that regimes with a radiative warming effect on the atmosphere also produce the largest implied latent heat. Taken as a whole, the results of the study corroborate the usefulness of the cloud regime concept, reaffirm the fundamental nature of the regimes as appropriate building blocks for cloud system classification, clarify their association with standard cloud types, and underscore their distinct radiative and hydrological signatures.

radiation↗

A preliminary study of the tropical water cycle and its sensitivity to surface warming

The Goddard Cumulus Ensemble Model (GCEM) has been used to demonstrate that cumulus-scale dynamics and microphysics play a major role in determining the vertical distribution of water vapor and clouds in the tropical atmosphere. The GCEM is described and is the basic structure of cumulus convection. The long-term equilibrium response to tropical convection to surface warming is examined. A picture of the water cycle within tropical cumulus clusters is developed.

Lau, K. M.↗

The M2 Proton Channel of Influenza Virus: How Does It Work?

The transport of protons across membranes is an essential process for both bioenergetics of modem cells and the origins of cellular life. All living systems make use of proton gradients across cell walls to convert environmental energy into a high-energy chemical compound, adenosine triphosphate (ATR), synthesized from adenosine diphosphate. ATR, in turn, is used as a source of energy to drive many cellular reactions. The ubiquity of this process in biology suggests that even the earliest cellular systems were relying on proton gradient for harvesting environmental energy needed to support their survival and growth. In contemporary cells, proton transfer is assisted by large, complex proteins embedded in membranes. The issue addressed in this study was: how the same process can be accomplished with the aid of similar, but much simpler molecules that could have existed in the protobiological milieu? The model system used in the study contained a bilayer membrane made of phospholipid, dimyristoylphosphatidylcholine (DMPC), which is a good model of the biological membranes focusing cellular boundaries. Both sides of the bilayer were surrounded by water which simulated the environment inside and outside the cell. Embedded in the membrane was a fragment of the Influenza-A M2 protein and enough sodium counterions to maintain system neutrality. This protein has been shown to exhibit remarkably high rates of proton transport and, therefore, is an excellent model to study the formation of proton gradients across membranes. The Influenza M2 protein is 97 amino acids in length, but a fragment 25 amino acids long, which contains a transmembrane domain of 19 amino acids flanked by 3 amino acids on each side, is sufficient to transport protons. Four identical protein fragments, each folded into a helix, aggregate to form small channels spanning the membrane. Protons are conducted through a narrow pore in the middle of the channel in response to applied voltage. This channel is large enough to contain water molecules, and is normally filled with water. In analogy to the mechanism of proton transfer in some other channels, it has been postulated that protons are translocated along the network of water molecules filling the pore of the channel. This mechanism, however, must involve an additional, important step because the channel contains four histidine amino acid residues, one from each of the helices, which are sufficiently large to occlude the pore and interrupt the water network. The histidine residues ensure channel selectivity by blocking transport of small such as sodium or potassium. They have been also implicated in gating protons due to the ability of each histidine to become positively charged by accepting an additional proton. Two mechanisms of gating have been proposed. In one mechanism, all four histidines acquire an additional proton and, due to repulsion between their positive charges, move away from one another, thus opening the channel. The alternative mechanism relies of the ability of protons to move between different atoms in a molecule (tautomerization). Thus, a proton is captured on one side of the gate while another proton is released from the opposite side, and the molecule returns to the initial state through tautomerization. The simulations were designed to test these two mechanisms. Large-scale, atomic-level molecular dynamics simulations of the channel, in which the histidine residues were in different protonation states revealed that all intermediate states of the system involved in the tautomerization mechanism are structurally stable and the arrangement of water molecules in the channel is conducive to the proton transport. In contrast, in the four-protonated state, postulated to exist in the gate-opening mechanism, the electrostatic repulsion between the histidine residues appears to be so large that the channel looses its structural integrity and one helix moves away from the remaining three. This result indicates that such a mechanism of proton transport is unlikely. The simulations revealed that translocation along a network of water molecules in the channel and tautomerization of the histidine residues in the M2 proteins in the most likely mechanism of proton transport. The results not only explain how a remarkably simple protein system can efficiently aid in the formation of proton gradients across cell walls, but also suggest how this system can be genetically re-engineered to become a directional, reversible proton pump. Such a pump can provide energy to laboratory-built models of simple cellular systems. If they were successfully constructed it would greatly advance our understanding of the beginnings of life and find important applications in medicine and pharmacology.

Pohorille, Andrew↗

Excerpts from Test Films: Langley Impacting Structures Facility, Lunar Module

The film includes excerpts from three studies: (1) Landing characteristics of a dynamic model of the HL-10 manned lifting entry vehicle, conducted by Sandy M. Stubbs, in which the vehicle landed on water at horizontal velocities of 240- and 250-feet per second (ft/sec). (2) Dynamic model investigation of water pressures and accelerations encountered during landings of the Apollo spacecraft conducted by Sandy M. Stubbs, in which horizontal velocity was 50 ft/sec. and pitch attitude was -12 and -28 degrees. (3) Comparative landing impact tests of a 1/6-scale model as a free body under earth gravity and a tethered full-scale lunar module on the Lunar Gravity Simulator. Landing 8 is shown, with a vertical velocity of 10 ft/sec. and a horizontal velocity of 8 ft/sec. Motion pictures were taken at 400 and 64 pps.

Source record↗

Results from ORACLES-2016: Observed Biomass Burning Aerosol Properties over the Southeast Atlantic Ocean and the Relationship to Meteorology

The Southeast Atlantic Ocean (SEA), with seasonal biomass burning (BB) smoke plumes overlying persistent stratocumulus cloud deck, offers an excellent natural laboratory to make the observations necessary to understand the complexities of aerosol-cloud-radiation interactions. The first field deployment of the NASA ORACLES (ObseRvations of Aerosols above CLouds and their intEractionS) campaign was conducted in September of 2016 out of Walvis Bay, Namibia. During this deployment, two NASA aircraft (a P-3 and an ER-2) were flown with a suite of aerosol, cloud, radiation, and meteorological instruments for remote-sensing and in-situ observations. In this talk, I will first present results from an intercomparison of the different instrument retrievals for the 2016 flights, with a specific focus on the measures of aerosol absorption. In ORACLES, data collected by eight different instrument teams are used to derive aerosol properties over this region by independent methods. We focus on the single scattering albedo (SSA), a measure of the relative absorption versus total extinction by aerosols, as the main comparable property between all methods. I will also discuss comparisons of the retrieved aerosol absorption parameters, as well as the agreement and the variability seen between different instruments, and spatially over the study region. Another important factor in understanding aerosol radiative effects is how the magnitude of these effects may be modified by meteorological conditions. I will next describe observations collected from the P-3 aircraft (from near-surface up to 6-7 kilometers) which show a strong correlation between the in-situ pollution indicators (carbon monoxide and aerosol properties) and atmospheric water vapor content, seen at all altitudes above the boundary layer. This condition is seen to persist over all flights, with minimal detrainment during advection from the continental source. In exploring the potential causal factors behind and implications of this relationship, we see indications that convective dynamics over the continent likely contribute to this elevated signal, but neither meteorological reanalysis nor trajectory analysis fully capture the magnitude and vertical structure of the observed elevated signal. Finally, I will discuss the radiative implications of the observed correlations, a topic of ongoing analysis in ORACLES: understanding the mechanisms which cause water vapor to covary with plume strength is important to quantifying the radiative effects (direct and semi-direct) of biomass burning aerosol in the region.

Pistone, Kristina↗

Interaction of a Model Peptide with a Water--Bilayer System

We discuss a molecular dynamics study of the alanine dipeptide at the interface between water and a glycerol-1-monooleate (GMO) bilayer. The dipeptide is interfacially active and incorporates into the bilayer without disrupting its structure. The interfacial region that is readily penetrated by the dipeptide spans the entire head group portion of the bilayer. The polar groups of the alanine dipeptide mostly remain well solvated by water and the oxygen atoms of GMO, and conformations of the dipeptide are characterized by (phi, psi) angles typical of alpha-helix and beta-sheet structures. When the molecule is deeper in the bilayer, the C(sub 7eq) state also becomes stable. The barrier to the isomerization reaction at the interface is lower than in bulk phases. After 7 ns of trajectories, the system is not fully equilibrated, due to slow collective motions involving GMO head groups. These result in decreased mobility and lower rates of isomerization of the dipeptide at the interface.

Pohorille, A.↗

Three-dimensional evolution of water vapor distributions in the Northern Hemisphere stratosphere as observed by the MLS

The three-dimensional evolution of stratospheric water vapor distributions observed by the Microwave Limb Sounder (MLS) during the period October 1991 - July 1992 is documented. The transport features inferred from the MLS water vapor distributions are corroborated using other dynamical fields, namely, nitrous oxide from the Cryogenic Limb Array Etalon Spectrometer instrument, analyzed winds from the U.K. Meteorological Office (UKMO), UKMO-derived potential vorticity, and the diabatic heating field. By taking a vortex-centered view and an along-track view, the authors observe in great detail the vertical and horizontal structure of the northern winter stratosphere. It is demonstrated that the water vapor distributions show clear signatures of the effects of diabatic descent through isentropic surfaces and quasi-horizontal transport along isentropic surfaces, and that the large-scale winter flow is organized by the interaction between the westerly polar vortex and the Aleutian high.

Lahoz, W. A.↗