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Nannochloropsis oceanica IMET1 and its bacterial symbionts for carbon capture, utilization, and storage: biomass and calcium carbonate production under high pH and high alkalinity

ABSTRACT To combat the increasing levels of carbon dioxide (CO 2 ) released from the combustion of fossil fuels, microalgae have emerged as a promising strategy for biological carbon capture, utilization, and storage. This study used a marine microalgal strain, Nannochloropsis oceanica IMET1, which thrives in high CO 2 concentrations. A high-pH, high-alkalinity culture was designed for CO 2 capture through algal biomass production as well as permanent sequestration through calcium carbonate (CaCO 3 ) precipitation. This was accomplished by timed pH elevation and the addition of sodium bicarbonate to cultures of N. oceanica grown at lab scale (1 L) and pilot scale (500 L) with 10% and 5% CO 2 , respectively. Our data showed that 0.02 M NaHCO 3 promoted algal growth and that sparging cultures with ambient air after 12 days raised pH and created favorable CaCO 3 formation conditions. At the 1 L scale, we reached 1.52 g L −1 biomass after 12 days and an extra 9.3% CO 2 was captured in the form of CaCO 3 precipitates. At the 500 L pilot scale, an extra 60% CO 2 was captured (Day 40) with a maximum CO 2 capture rate of 63.2 g m −2 day −1 (Day 35). Bacterial communities associated with the microalgae were dominated by two novel Patescibacteria. Functional analysis revealed that genes for several plant growth-promotion traits (PGPTs) were enriched within this group. The microalgal-bacterial coculture system offers advantages for enhanced carbon mitigation through biomass production and simultaneous precipitation of recalcitrant CaCO 3 for long-term CO 2 storage. IMPORTANCE Capturing carbon dioxide (CO 2 ) released from fossil fuel combustion is of the utmost importance as the impacts of climate change continue to worsen. Microalgae can remove CO 2 through their natural photosynthetic pathways and are additionally able to convert CO 2 into a stable, recalcitrant form as calcium carbonate (CaCO 3 ). We demonstrate that microalgae-based carbon capture systems can be greatly improved with high pH and high alkalinity by providing optimal conditions for carbonate precipitation. Our results with the microalga, Nannochloropsis oceanica strain IMET1, show an extra 9.3% CO 2 captured as CaCO 3 at the 1 L scale and an extra 60% CO 2 captured at the 500 L (pilot) scale. Our optimized system provides a novel approach to capture CO 2 through two mechanisms: (i) as organic carbon within microalgal biomass and (ii) as inorganic carbon stored permanently in the form of CaCO 3.

20 FOSSIL-FUELED POWER PLANTS↗

Preliminary results of t and f asymmetries for {eta} photoproduction from the proton

We present preliminary data for the T and F asymmetries in {eta} photoproduction from the proton, along with comparisons to theoretical predictions. The data used in the present analysis were taken using the CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Lab, a cryogenic target which utilized transversely polarized protons in a butanol target, and incident tagged photons with energy between 0.62 and 2.93 GeV.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Identifying Key Proteins in Hg Methylation Pathways of Desulfovibrio by Global Proteomics (Final Report)

Hg is a wide-spread contaminant in the environment and is toxic in all of its various forms. Organomercurials (RHg + ) and mercuric ion (Hg 2+ ) directly inactivate proteins by binding to their cysteine or selenocysteine residues. In our previous collaborative project involving the Summers, Miller and Lipton laboratories, we developed a high-throughput, mass spectrometry-based global proteomics approach using the 7 stable isotopes of Hg to identify proteins from phenylmercury-exposed E. coli cells that form stable PhHg-adducts. The two overall objectives of this subsequent collaborative project were (1) to expand our novel organomercury-proteomics capability for use with inorganic ionic (HgX 2 ) exposure and (2) to use this new capability to identify Hg-protein adducts in methylating and non-methylating Desulfovibrio exposed to inorganic ionic HgX 2 and test whether those identified uniquely in methylating bacteria are involved in the methylation pathway. The specific objectives and accomplishments of the Miller lab in this project included: Development of algorithms for analysis of the Hg-proteomic mass spectrometry data to identify and quantify Hg-adducted peptides and other trends in the data.

59 BASIC BIOLOGICAL SCIENCES↗

TEAMER - Field Demonstration of MarineSitu’s Marine Energy Monitoring Tools - CRADA 664 (Abstract)

In order to effectively monitor for marine life around marine energy devices and thus minimize the risk of collision, multiple sensors working in coordination and augmented with around-the-clock automated monitoring algorithms need to be installed in challenging high-energy tidal and wave environments. Such systems are often too expensive for widespread adoption, or lack sufficient sensors or smarts to enable around-the-clock, real-time monitoring without human involvement. MarineSitu has been working to tackle this problem by developing a low-cost, combined sonar and stereo camera sensor array with connected real-time AI-based algorithms for automatically detecting marine life in these marine energy suitable environments. In this TEAMER project with Pacific Northwest National Lab (PNNL), MarineSitu will be testing this novel sensor system for the first time in the high-energy tidal channel environment at PNNL’s Marine and Coastal Research Lab. Throughout this deployment, MarineSitu will be monitoring their system and running analytics on the sensor’s data in real-time. Meanwhile, PNNL Data Scientists and Ocean Engineers, will be evaluating the system’s effectiveness and ease of use both as a tool for plug-and-play environmental monitoring and novel environmental monitoring research. In doing so, the team will improve MarineSitu’s system and software, produce insightful data products, and develop novel visualizations and AI algorithms for combining and analyzing the data produced by systems like MarineSitu’s.

16 TIDAL AND WAVE POWER↗

TEAMER – Field Demonstration of MarineSitu’s Marine Energy Monitoring (Abstract)

In order to effectively monitor for marine life around marine energy devices and thus minimize the risk of collision, multiple sensors working in coordination and augmented with around-the-clock automated monitoring algorithms need to be installed in challenging high-energy tidal and wave environments. Such systems are often too expensive for widespread adoption, or lack sufficient sensors or smarts to enable around-the-clock, real-time monitoring without human involvement. MarineSitu has been working to tackle this problem by developing a low-cost, combined sonar and stereo camera sensor array with connected real-time AI-based algorithms for automatically detecting marine life in these marine energy suitable environments. In this TEAMER project with Pacific Northwest National Lab (PNNL), MarineSitu will be testing this novel sensor system for the first time in the high-energy tidal channel environment at PNNL’s Marine and Coastal Research Lab. Throughout this deployment, MarineSitu will be monitoring their system and running analytics on the sensor’s data in real-time. Meanwhile, PNNL Data Scientists and Ocean Engineers, will be evaluating the system’s effectiveness and ease of use both as a tool for plug-and-play environmental monitoring and novel environmental monitoring research. In doing so, the team will improve MarineSitu’s system and software, produce insightful data products, and develop novel visualizations and AI algorithms for combining and analyzing the data produced by systems like MarineSitu’s.

16 TIDAL AND WAVE POWER↗

STANDARDIZATION OF CEBAF 12 GEV UPGRADE CAVITY TESTING

CEBAF 12GeV upgrade project includes 80 new 7-cell cavities to form 10 cryomodules. Each cavity underwent RF qualification at 2.07K using a high power accelerating gradient test and an HOM survey in Jefferson Lab's Vertical Testing Area (VTA) before cavity string assembly. In order to ensure consistently high quality data, updated cavity testing procedures and analysis were implemented and used by a group of VTA operators. For high power tests, a cavity testing procedure was developed and used in conjunction with a LabVIEW program to collect the test data. Additionally while the cavity was at 2.07K, an HOM survey was performed using a network analyzer and a combination of Excel and Mathematica programs. Data analysis was standardized and an online logbook, Pansophy, was used for data storage and mining. The Pansophy system allowed test results to be easily summarized and searchable across all cavity tests. In this presentation, the CEBAF 12GeV upgrade cavity testing procedure, method for data analysis, and results reporting results will be discussed.

43 PARTICLE ACCELERATORS↗

Analyzing Real-World Light Duty Vehicle Efficiency Benefits

Off-cycle technologies represent an important pathway to achieve real-world fuel savings, through which OEMs can potentially receive credit toward CAFE compliance. DOE national labs such as NREL are well positioned to provide objective input on these technologies using large, national data sets in conjunction with OEM- and technology-specific testing. This project demonstrates an approach that combines vehicle testing (dynamometer and on-road) with powertrain modeling and simulation over large, representative datasets to quantify real-world fuel economy. The approach can be applied to specific off-cycle technologies (engine encapsulation, start/stop, connected vehicle, etc.) in A/B comparisons to support calculation of realistic real-world impacts. Future work will focus on testing-based A/B technology comparisons that demonstrate the significance of this approach.

33 ADVANCED PROPULSION SYSTEMS↗

(Oxy)hydroxides Formed on Aluminum Fuel Materials After Irradiation and Long-Term Wet Storage - 20354

The aluminum cladding of research-reactor fuel experiences general corrosion when in contact with water during in-reactor service and post-discharge wet storage, resulting in the formation of adherent aluminum (oxy)hydroxide films. These (oxy)hydroxides contain chemically-bound water that poses challenges for extended dry storage due to the risk of thermal or radiolytic decomposition releasing free water and/or hydrogen and oxygen gases. This study describes characterization of the (oxy)hydroxides present on several aluminum materials used in reactor operation and subsequently stored wet in the L-Basin storage facility at the Savannah River Site (SRS) for an extended period. Characterization data providing insight into the loading, composition, and morphology of (oxy)hydroxides to be expected on service-exposed aluminum cladding provides valuable benchmarks for designing adequate drying and dry-storage approaches. This work is part of a broader investigation to address knowledge gaps and technical data needs for dry storage of aluminum-clad spent nuclear fuel (ASNF), which included in-lab growth of (oxy)hydroxide films on aluminum alloy substrates to investigate formation behavior, investigation of drying methods to remove existing (oxy)hydroxides from ASNF cladding, and measurement of radiolytic yield of hydrogen from (oxy)hydroxide powders and films. In this study, (oxy)hydroxide films were characterized for three aluminum-alloy materials used in reactors and subsequently stored wet for up to approximately 40 years in L-Basin at SRS: one cropping from a Missouri University Research Reactor (MURR) fuel element (Al-6061 alloy), one cropping from a Universal Sleeve Housing (USH) (Al-6063 alloy), and one Mark-16B fuel assembly (either Al-6061 or Al- 6063). The USH and Mark-16B were used in SRS production reactors. Characterization of the as-received (oxy)hydroxides included scanning electron microscopy (SEM) in both plan-view and cross-section to characterize the (oxy)hydroxide layer's morphology, thickness, and structure. X-ray diffraction (XRD) was used to identify the chemical composition and distinguish between the various aluminum (oxy)hydroxides known to form under reactor and storage conditions. XRD analysis revealed both bayerite (Al(OH)3) and boehmite (AlOOH) on the surface of the MURR and USH samples, as well as bayerite, boehmite, and gibbsite (another Al(OH)3 polymorph) on the surface of the Mark-16B sample. The aluminum trihydroxides, bayerite and gibbsite, are typically associated with corrosion in low-temperature (<80 deg. C) water, while boehmite is expected to form at higher water temperature (>80 deg. C). The presence of bayerite on the USH, which is believed to have operated close to 90 deg. C, suggests that boehmite formed during in-reactor exposure was not protective against further hydroxide growth in low-temperature wet storage. Cross-section scanning electron microscopy (SEM) showed total (oxy)hydroxide layer thicknesses of ∼5- 10 μm for the MURR and ∼5-15 μm for the Mark-16B. The thickness of the USH's (oxy)hydroxide layer was indiscernible by the current mounting and imaging method, despite plan-view SEM and XRD confirming the presence of an (oxy)hydroxide layer. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Parity Violation Inelastic Scattering Experiments at 6 GeV and 12 GeV Jefferson Lab

We report on the measurement of parity-violating asymmetries in the deep inelastic scattering and nucleon resonance regions using inclusive scattering of longitudinally polarized electrons from an unpolarized deuterium target. The effective weak couplings C$_{2q}$ are accessible through the deep-inelastic scattering measurements. Here we report a measurement of the parity-violating asymmetry, which yields a determination of 2C$_{2u}$ - C$_{2d}$ with an improved precision of a factor of five relative to the previous result. This result indicates evidence with 95% confidence that the 2C$_{2u}$ - C$_{2d}$ is non-zero. This experiment also provides the first parity-violation data covering the whole resonance region, which provide constraints on nucleon resonance models. Finally, the program to extend these measurements at Jefferson Lab in the 12 GeV era using the Solenoidal Large Intensity Device was also discussed.

Sulkosky, Vincent A.↗

Disaggregating Future Retail Electricity Rate Growth [Slides]

Recent Berkeley Lab research found that modest retail rate increases over the past 10 years were mostly driven by large increases in capital expenditures (CapEx) that were offset in part by substantial wholesale price reductions. Decision-makers are increasingly concerned about the potential future rate impacts of a number of policies and industry trends that support rapid decarbonization, electrification, and grid modernization. Using historical FERC Form 1 data and the existing literature on policies and industry trends that are likely to affect utility-incurred costs and retail sales, Berkeley Lab researchers developed ranges of forecasted growth rates for cost-related rate drivers (i.e., fuel and purchased power; transmission, distribution, generation, and other categories of both non-fuel operations & maintenance and CapEx) and non-cost related rate drivers (i.e., retail sales, peak demand, and customers). These were then used as inputs to a pro-forma utility financial model (FINDER) that estimated the growth in retail electric rates between 2020 and 2030 for a prototypical vertically-integrated investor-owned utility in the United States. The analysis produced the following results: 1. Assuming average growth rates in all rate drivers, future retail rate growth is driven by sizable increases in all CapEx costs, where fuel and purchased power costs are replaced by generation CapEx as the largest rate component between 2020 and 2030. 2. Growth in sales/peak demand/customers, generation CapEx costs, and fuel and purchased power (FPP) costs, in isolation, produce the most uncertainty in rate growth. Specifically, a 1% increase in the compound annual growth rate (CAGR) of retail sales, coincident peak demand (CP), and customers (Sales-CP-Cust) results in a 0.88-0.93% decrease in the CAGR of rates, in isolation. However, a 1% increase in the CAGR of generation CapEx budgets results in a 0.07-0.14% increase in the CAGR of rates, while a 1% increase in the CAGR of FPP costs causes a 0.10-0.14% increase in the CAGR of rates, all else being equal. 3. Taking into account the correlation and variability of the growth in all rate drivers jointly, generation CapEx is expected to be both the largest and most uncertain rate component by 2030 (20-25% share of the retail rate). Transmission and distribution CapEx, along with fuel and purchased power costs are each expected to comprise between 12% and 17% of retail rates.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Studies of Nucleon Form Factors with 12 GeV CEBAF and SuperBigBite

The elastic electromagnetic form factors are among the most fundamental quantities that describe the ground-state structure of the proton and neutron. Precision data of the form factors over a wide kinematical range provide a powerful test of current theories of hadron structure. A number of experiments aiming to measure the electric and magnetic elastic form factors of the neutron, G{sub E}{sup n} and G{sub M}{sup n}, and proton, G{sub E}{sup p}, at very high momentum transfer, up to the range of Q{sup 2} = 10-14 (GeV/c){sup 2}, are planned to be carried out with the future 11 GeV electron beam of the upgraded CEBAF at Jefferson Lab. These experiments will determine the nucleon form factors with unprecedented precision to Q{sup 2}-values up to three times higher than those of existing data. We review the approved proposals and the conceptual design of a new spectrometer, SuperBigBite, that will be used in these and other future experiments at Jefferson Lab.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Precision Measurements of Neutral Pion Electroproduction Near Threshold: A Test of Chiral QCD Dynamics

Preliminary results are presented from an experiment to measure {pi}{sup 0} electroproduction at and above threshold using the p(e;e&#x27; p){pi}{sup 0} reaction. The data were taken at a beam energy of 1192 MeV using a two-spectrometer setup in Hall A at Jefferson Lab. For the first time in {pi}{sup 0} threshold electroproduction, complete coverage of the {phi}{sub {pi}}* and {theta}{sub {pi}}* angles in the center-of-mass (C.M.) was obtained for the invariant mass region up to {Delta}W=18 MeV above the {pi}{sup 0} threshold. At the same time our invariant momentum transfer squared covers the range Q{sup 2} = 0.05-0.15 (GeV/c){sup 2} with twelve bins in Q{sup 2}. The improved kinematic coverage in C.M., W and Q{sup 2} will better constrain theoretical interpretations of the data using phenomenological models and QCD-inspired models such as Heavy Baryon Chiral Perturbation Theory.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

North Slope Alaska and Tethered Balloon Systems: ARM Facilities Monthly Status Update

In support of the DOE Office of Science, Sandia National Labs is one of nine national laboratories which oversees the Atmospheric Radiation Measurement (ARM) Program. The Sandia ARM team has an obligation to fulfill its mission to provide the nation with data to improve the understanding of climate processes and the representation of those processes in climate models. Sandia’s ARM team’s ability to provide detailed and accurate descriptions of the Earth’s atmosphere in diverse climate regimes assists the DOE in development of sustainable solutions to the nation’s energy and environmental challenges. Sandia Labs manages ARM atmospheric facilities along the North Slope of Alaska (NSA) at Utqiagvik as well as the Tethered Balloon System (TBS). Activities conducted at NSA and with the TBS aide in data collection for the ARM data archive. An overview of these activities for the month of December follows.

54 ENVIRONMENTAL SCIENCES↗

A DEEP NARROWBAND IMAGING SEARCH FOR C iv AND He ii EMISSION FROM Lyα BLOBS

We conduct a deep narrowband imaging survey of 13 Lyα blobs (LABs) located in the SSA22 proto-cluster at z ∼ 3.1 in the C iv and He ii emission lines in an effort to constrain the physical process powering the Lyα emission in LABs. Our observations probe down to unprecedented surface brightness (SB) limits of (2.1–3.4) × 10{sup −18} erg s{sup −1} cm{sup −2} arcsec{sup −2} per 1 arcsec{sup 2} aperture (5σ) for the He ii λ1640 and C iv λ1549 lines, respectively. We do not detect extended He ii and C iv emission in any of the LABs, placing strong upper limits on the He ii/Lyα and C iv/Lyα line ratios, of 0.11 and 0.16, for the brightest two LABs in the field. We conduct detailed photoionization modeling of the expected line ratios and find that, although our data constitute the deepest ever observations of these lines, they are still not deep enough to rule out a scenario where the Lyα emission is powered by the ionizing radiation from an obscured active galactic nucleus. Our models can accommodate He ii/Lyα and C iv/Lyα ratios as low as ≃0.05 and ≃0.07, respectively, implying that one needs to reach SB as low as (1–1.5) × 10{sup −18} erg s{sup −1} cm{sup −2} arcsec{sup −2} (at 5σ) in order to rule out a photoionization scenario. These depths will be achievable with the new generation of image-slicing integral field units such as the Multi Unit Spectroscopic Explorer (MUSE) on VLT and the Keck Cosmic Web Imager (KCWI). We also model the expected He ii/Lyα and C iv/Lyα in a different scenario, where Lyα emission is powered by shocks generated in a large-scale superwind, but find that our observational constraints can only be met for shock velocities v{sub s} ≳ 250 km s{sup −1}, which appear to be in conflict with recent observations of quiescent kinematics in LABs.

79 ASTRONOMY AND ASTROPHYSICS↗

Performance Audit Report: Automated Meteorological Monitoring Stations, Lawrence Livermore National Laboratory

The Waste and Air Quality Offices Group at the Lawrence Livermore National Laboratory operates two main meteorological monitoring sites near Livermore, California. The primary met tower (Site 200) is located in the northwest corner of the lab and consists of a fifty-two (52) meter tower with vertical wind speed sensors, horizontal wind speed and direction sensors and air temperature sensors at fifty-two (52) meters, twenty-three (23) meters and ten (10) meters. In addition, there is a temperature and humidity probe at ten (10) meters. At two (2) meters there are upward and downward facing solar and infrared radiation sensors, an air temperature sensor, barometric pressure sensor and a temperature and relative humidity probe. At ground level there is a tipping bucket rain gauge located to the southwest of the tower. The datalogger, communication peripherals and the barometric pressure sensor are housed in a small building near the tower. The Site 300 met site is located approximately 10 miles east of Livermore within the boundary of the LLNL blast test site. This site also consists of a 52-meter tower which is configured the same as the 52-meter tower at Site 200. There is also a building at this site that houses the datalogger, communication peripherals and the barometric pressure sensor. At both Site 200 and Site 300, data are collected by Ethernet with telephone modem collection as a backup. The data collection interval for both sites is fifteen (15) minutes via workstations running Loggernet software located at the main lab facility. The audits of the Lawrence Livermore National Laboratory’s two automated meteorological stations located near Livermore, California were performed on October 24 & 25, 2023. These audits were performed under the guidelines of the Quality Assurance Handbook for Air Pollution Measurement Systems, Volume IV, Version 2 and the On-Site Meteorological Program Guidance for Regulatory Modeling Applications, U.S. EPA.

54 ENVIRONMENTAL SCIENCES↗

Deep glassy state dynamic data challenge glass models: Configurational entropy models

This work was the result of the student's (Dongjie Chen) qualifying exam response. In it the experimental observation from our labs that the relaxation times of ultrastable glasses (a 20 million year old amber and a vapor deposited amorphous teflon) do not follow the expected Vogel-Fulcher or WLF types of divergence as the temperatures fall below the glass transition temperature. In the work we evaluated several theories by considering them in different ways. We used them as generally used and show that they do not capture the non-diverging behavior. Because the models were all entropy-based models, we also considered the possibility that the temperature dependence of the entropy used in the models is incorrect and chose two ways of estimating the entropy that do not follow the classical theoretical models. First we used the actually measured calorimetric response for the two ultrastable glasses, and we used the Milchev model, which does not go to zero above 0 Kelvin. Both of these approaches led to improved agreement between the theories and the experimental data. Finally, we also asked what the temperature dependence of the entropy would have to be so that the models agreed with the observed data. In some cases the entropy so-calculated was reasonable, while in others it was unphysical. In sum, the important point of the work is that the observation of non-diverging time-scales that suggest that there is no ideal glass transition can be reasonably captured by current theories if they take more realistic models of the entropy than is usually the case.

36 MATERIALS SCIENCE↗

Modeling the Echelle Spectra Continuum with Alpha Shapes and Local Regression Fitting

Continuum normalization of echelle spectra is an important data analysis step that is difficult to automate. Polynomial fitting requires a reasonably high-order model to follow the steep slope of the blaze function. However, in the presence of deep spectral lines, a high-order polynomial fit can result in ripples in the normalized continuum that increase errors in spectral analysis. Here, we present two algorithms for flattening the spectrum continuum. The Alpha-shape Fitting to Spectrum algorithm is completely data driven, using an alpha shape to obtain an initial estimate of the blaze function. The Alpha-shape and Lab Source Fitting to Spectrum algorithm incorporates a continuum constraint from a laboratory source reference spectrum for the blaze function estimation. These algorithms are tested on a simulated spectrum, where we demonstrate improved normalization compared to polynomial regression for continuum fitting. We show an additional application, using the algorithms for mitigation of spatially correlated quantum efficiency variations and fringing in the charge-coupled device detector of the EXtreme PREcision Spectrometer.

79 ASTRONOMY AND ASTROPHYSICS↗