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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

Multiomic atlas with functional stratification and developmental dynamics of zebrafish cis -regulatory elements

Zebrafish, a popular organism for studying embryonic development and for modeling human diseases, has so far lacked a systematic functional annotation program akin to those in other animal models. To address this, we formed the international DANIO-CODE consortium and created a central repository to store and process zebrafish developmental functional genomic data. Our data coordination center (https://danio-code.zfin.org) combines a total of 1,802 sets of unpublished and re-analyzed published genomic data, which we used to improve existing annotations and show its utility in experimental design. We identified over 140,000 cis-regulatory elements throughout development, including classes with distinct features dependent on their activity in time and space. We delineated the distinct distance topology and chromatin features between regulatory elements active during zygotic genome activation and those active during organogenesis. Finally, we matched regulatory elements and epigenomic landscapes between zebrafish and mouse and predicted functional relationships between them beyond sequence similarity, thus extending the utility of zebrafish developmental genomics to mammals.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2019

Electrostatic subframing and compressive-sensing video in transmission electron microscopy

We present kilohertz-scale video capture rates in a transmission electron microscope, using a camera normally limited to hertz-scale acquisition. An electrostatic deflector rasters a discrete array of images over a large camera, decoupling the acquisition time per subframe from the camera readout time. Total-variation regularization allows features in overlapping subframes to be correctly placed in each frame. Moreover, the system can be operated in a compressive-sensing video mode, whereby the deflections are performed in a known pseudorandom sequence. Compressive sensing in effect performs data compression before the readout, such that the video resulting from the reconstruction can have substantially more total pixels than that were read from the camera. This allows, for example, 100 frames of video to be encoded and reconstructed using only 15 captured subframes in a single camera exposure. We demonstrate experimental tests including laser-driven melting/dewetting, sintering, and grain coarsening of nanostructured gold, with reconstructed video rates up to 10 kHz. The results exemplify the power of the technique by showing that it can be used to study the fundamentally different temporal behavior for the three different physical processes. Both sintering and coarsening exhibited self-limiting behavior, whereby the process essentially stopped even while the heating laser continued to strike the material. We attribute this to changes in laser absorption and to processes inherent to thin-film coarsening. In contrast, the dewetting proceeded at a relatively uniform rate after an initial incubation time consistent with the establishment of a steady-state temperature profile.

36 MATERIALS SCIENCE↗
DOE OSTI2018

Distinctive structural basis for DNA recognition by the fission yeast Zn 2 Cys 6 transcription factor Pho7 and its role in phosphate homeostasis

Pho7, a member of the Zn 2 Cys 6 family of fungal transcription factors, is the key transcriptional activator underlying fission yeast phosphate homeostasis, a physiological response to phosphate starvation in which the pho1, pho84 and tgp1 genes are upregulated. Here, we delineated a minimized 61-amino-acid Pho7 DNA-binding domain (DBD) and determined the 1.7 Å crystal structure of the DBD at its target site in the tgp1 promoter. Two distinctive features of the Pho7 DBD are: it binds DNA as a monomer, unlike most other fungal zinc-cluster factors that bind as homodimers; and it makes extensive interactions with its asymmetric target sequence over a 14-bp footprint that entails hydrogen bonding to 13 individual bases within, and remote from, the CGG triplet typically recognized by other Zn 2 Cys 6 DBDs. Base pair substitutions at Pho7 sites in the tgp1 and pho1 promoters highlight the importance of the 5'-CGG triplet for Pho7 binding in vitro and Pho7-dependent gene expression in vivo. We identify several DBD amino acids at which alanine substitution effaced or attenuated the pho1 phosphate starvation response and concordantly reduced Pho7 binding to a pho1 promoter site.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Microbiome dynamics in the congregate environment of U.S. Army Infantry training

Within military training and operational environments, individuals from diverse backgrounds share common spaces, follow structured routines and diets, and engage in physically demanding tasks. While there has been interest in leveraging microbiome features to predict and improve military health and performance, the longitudinal convergence of microbiomes in such constrained environments has not been established. To assess the degree of microbiome convergence, we performed shotgun metagenomic sequencing on swab samples from a military trainee cohort. Samples were taken across four different body sites, three timepoints, and two spatially distinct platoons. We observed evidence of convergence in one platoon, whereby similarity in microbiome composition increased over time, with numerous differentially abundant species. We found no indication of strain transfer between individuals, suggesting that convergence was influenced by external environmental factors, diet, and lifestyle. Microbial shifts observed in the convergence process included a decrease in fungal species, such as Malassezia restricta in nasal cavities, and a decrease in Prevotella species at inguinal regions across time. Shifts in multiple Corynebacterium species were also observed with varying magnitudes depending on the body site. Overall, we provide preliminary evidence of convergence of host microbial communities in military-associated environments that were distinguishable using shotgun metagenomic sequencing approaches. The data presented here on microbiome convergence, dynamics, and stability may inform risk-based mitigation in congregate military settings facilitating development of targeted microbial, dietary, or other interventions to optimize health and performance of military populations.

Biological and medical sciences↗
DOE OSTI2024

First Report and Molecular Variability of Belonolaimus longicaudatus Associated with Turfgrass in Maryland

Abstract Turfgrass is a crop used extensively in athletic fields and golf courses in Maryland. A soil sample collected in July 2023 from an athletic field in Baltimore County, Maryland, part of a turfgrass nematode survey, containedBelonolaimus longicaudatus. In the southeastern United States,B. longicaudatusis an economically important pathogen of warm season turfgrass. The density was four individuals/100 cm 3 of soil, and no visual symptoms were observed in the bermudagrass field. Morphological features and morphometrics of males and females were consistent withB. longicaudatusand placed the Maryland population in a subclade that was geographically represented by populations from north and west Florida, Texas, and South Carolina. Sequencing of the internal transcribed spacer region ITS1 and ITS2 and 28S large ribosomal subunit D2-23 expansion region confirmed the species' identity. Phylogenetic trees and parsimony network analysis placed the Maryland isolate in a large grouping ofB. longicaudatuspopulations including those from Alabama, Delaware, Florida, Indiana, Mississippi, South Carolina, and Texas. To our knowledge, this is the first report ofB. longicaudatusin Maryland.

Zoology↗
DOE OSTI2022

Phylogenomics and the first higher taxonomy of Placozoa, an ancient and enigmatic animal phylum

Placozoa is an ancient phylum of extraordinarily unusual animals: miniscule, ameboid creatures that lack most fundamental animal features. Despite high genetic diversity, only recently have the second and third species been named. While prior genomic studies suffer from incomplete placozoan taxon sampling, we more than double the count with protein sequences from seven key genomes and produce the first nuclear phylogenomic reconstruction of all major placozoan lineages. This leads us to the first complete Linnaean taxonomic classification of Placozoa, over a century after its discovery: This may be the only time in the 21st century when an entire higher taxonomy for a whole animal phylum is formalized. Our classification establishes 2 new classes, 4 new orders, 3 new families, 1 new genus, and 1 new species, namely classes Polyplacotomia and Uniplacotomia; orders Polyplacotomea, Trichoplacea, Cladhexea, and Hoilungea; families Polyplacotomidae, Cladtertiidae, and Hoilungidae; and genus Cladtertia with species Cladtertia collaboinventa, nov. Our likelihood and gene content tree topologies refine the relationships determined in previous studies. Adding morphological data into our phylogenomic matrices suggests sponges (Porifera) as the sister to other animals, indicating that modest data addition shifts this node away from comb jellies (Ctenophora). Furthermore, by adding the first genomic protein data of the exceptionally distinct and branching Polyplacotoma mediterranea , we solidify its position as sister to all other placozoans; a divergence we estimate to be over 400 million years old. Yet even this deep split sits on a long branch to other animals, suggesting a bottleneck event followed by diversification. Ancestral state reconstructions indicate large shifts in gene content within Placozoa, with Hoilungia hongkongensis and its closest relatives having the most unique genetics.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1973

Optimal space communications techniques

The design, development, and analysis are reported of a digital phase-locked loop (DPLL) for FM demodulation and threshold extension. One of the features of the developed DPLL is its synchronous, real time operation. The sampling frequency is constant and all the required arithmetic and logic operations are performed within one sampling period, generating an output sequence which is converted to analog form and filtered. An equation relating the sampling frequency to the carrier frequency must be satisfied to guarantee proper DPLL operation. The synchronous operation enables a time-shared operation of one DPLL to demodulate several FM signals simultaneously. In order to obtain information about the DPLL performance at low input signal-to-noise ratios, a model of an input noise spike was introduced, and the DPLL equation was solved using a digital computer. The spike model was successful in finding a second order DPLL which yielded a five db threshold extension beyond that of a first order DPLL.

Schilling, D. L.↗
NASA NTRS1974

Surface geology of the moon

Questions regarding the formation of major lunar surface features are discussed, giving attention to the multiringed circular basins, the craters, and the mare material. Aspects of lunar stratigraphy are explored. At the present time, a lunar-wide time-stratigraphic sequence exists, in which surface units belong to one of a number of systems, including the Copernican, the Eratosthenian, Imbrian, Nectarian, and pre-Nectarian system. Details of Apollo surface exploration as conducted in each individual mission are considered along with the chemistry and the age of lunar materials, the lunar interior, and the evolution of the lunar surface.

El-Baz, F.↗
NASA NTRS1978

Computation of three-dimensional nozzle-exhaust flow fields with the GIM code

A methodology is introduced for constructing numerical analogs of the partial differential equations of continuum mechanics. A general formulation is provided which permits classical finite element and many of the finite difference methods to be derived directly. The approach, termed the General Interpolants Method (GIM), can combined the best features of finite element and finite difference methods. A quasi-variational procedure is used to formulate the element equations, to introduce boundary conditions into the method and to provide a natural assembly sequence. A derivation is given in terms of general interpolation functions from this procedure. Example computations for transonic and supersonic flows in two and three dimensions are given to illustrate the utility of GIM. A three-dimensional nozzle-exhaust flow field is solved including interaction with the freestream and a coupled treatment of the shear layer. Potential applications of the GIM code to a variety of computational fluid dynamics problems is then discussed in terms of existing capability or by extension of the methodology.

Spradley, L. W.↗
NASA NTRS1984

NSSFC: VAS assessment activities

Vertical atmospheric sounder data were evaluated. Basic work thus far has been directed toward evaluating, over the in near real time to determine the instrument's ability to detect regions with potential for severe storm development. A real time hourly water vapor loop 16 hours daily provides data to monitor position and trends of mid and upper level meteorological features. Because water vapor is more uniform than clouds, the water vapor images improve the ability to monitor certain atmospheric features such as jets, shortwaves, vorticity centers, and subsidence zones. Additional products to be evaluated include a version of the split window developed by NASA and cloud drift winds generated from three hourly sequenced CO2 VAS channels. A technique also under evaluation at NSSFC provides hail size potential based on VAS sounding data. Part of a planned upgrade to CSIS linking the 9600 baud phone line to one of the CSIS CPUs to allow access to VAS data via CSIS.

Anthony, R. W.↗
NASA NTRS1984

The Venus Radar Mapper (VRM) mission

The Venus Radar Mapper (VRM) mission is sponsored by NASA to put a single spacecraft in orbit around Venus to map the surface of Venus using a synthetic aperture mapping radar. The spacecraft is scheduled to be launched in April 1988 using a Shuttle-Centaur G combination. The spacecraft arrives at Venus in late July 1988 and begins its mapping mission which lasts for one Venus rotation or 243 days. This paper describes the VRM mission at its present state of design. The science objectives and project constraints are described. Key features of the spacecraft system and radar system are discussed. The interplanetary and mapping orbit design are covered. Navigation strategy is explained, including trajectory maneuvers and mapping phase orbit determination. Finally, the mapping sequences to optimize planet coverage are described.

Cutting, E.↗
NASA NTRS1986

The extinction law in the open cluster NGC 457 and the intrinsic energy distribution of Phi Cassiopeiae (F0 Ia)

Five early B-type stars near the main-sequence turnoff in NGC 457 have been observed at low dispersion with the short-wavelength prime and the long-wavelength redundant cameras of the IUE satellite. The equivalent widths of spectral features that are particularly strong and sensitive to temperature and luminosity were computed in the cluster stars and in 20 lightly reddened stars of types O9-B3 and luminosity classes III-V. The comparison of the equivalent widths provides a reliable method for finding matching pairs. Having identified the best comparison star for each program star, binned fluxes were used to determine the mean extinction curve. In order to cover the visible region, monochromatic fluxes of Phi Cas were derived from observations with the intensified Reticon scanner mounted on the No. 2 0.9 m telescope of KPNO, and they were dereddened with the mean extinction curve of Savage and Mathis. Thus, the intrinsic energy distribution of Phi Cas were determined from 1500 to 5800 A for use in a detailed model-atmosphere analysis.

Rosenzweig, P.↗
NASA NTRS1996

The Beta Pictoris Phenomenon in A-Shell Stars: Detection of Accreting Gas

We present the results of an expanded survey of A-shell stars using IUE high-dispersion spectra and find accreting, circumstellar gas in the line of sight to nine stars, in addition to the previously identified beta Pic, HR 10, and 131 Tau, which can be followed to between +70 and 100 km/s relative to the star. Two of the program stars, HD 88195 and HD 148283, show variable high-velocity gas. Given the small number of IUE spectra for our program stars, detection of high-velocity, accreting gas in 2/3 of the A-shell stars sampled indicates that accretion is an intrinsic part of the A-shell phenomenon and that beta Pic is not unique among main-sequence A stars in exhibiting such activity. Our program stars, as a group, have smaller column densities of high-velocity gas and smaller near-IR excesses compared with beta Pic. These features are consistent with greater central clearing of a remnant debris disk, compared with beta Pic, and suggest that the majority of field A-shell stars are older than beta Pic.

Grady, C. A.↗
DOE OSTI2023

Discovery of a novel filamentous prophage in the genome of the Mimosa pudica microsymbiont Cupriavidus taiwanensis STM 6018

Integrated virus genomes (prophages) are commonly found in sequenced bacterial genomes but have rarely been described in detail for rhizobial genomes. Cupriavidus taiwanensis STM 6018 is a rhizobial Betaproteobacteria strain that was isolated in 2006 from a root nodule of a Mimosa pudica host in French Guiana, South America. Here we describe features of the genome of STM 6018, focusing on the characterization of two different types of prophages that have been identified in its genome. The draft genome of STM 6018 is 6,553,639 bp, and consists of 80 scaffolds, containing 5,864 protein-coding genes and 61 RNA genes. STM 6018 contains all the nodulation and nitrogen fixation gene clusters common to symbiotic Cupriavidus species; sharing >99.97% bp identity homology to the nod / nif / noeM gene clusters from C. taiwanensis LMG19424 T and “ Cupriavidus neocalidonicus” STM 6070. The STM 6018 genome contains the genomes of two prophages: one complete Mu-like capsular phage and one filamentous phage, which integrates into a putative dif site. This is the first characterization of a filamentous phage found within the genome of a rhizobial strain. Further examination of sequenced rhizobial genomes identified filamentous prophage sequences in several Beta-rhizobial strains but not in any Alphaproteobacterial rhizobia.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1989

Propagation of a westward traveling surge and the development of persistent auroral features

Imaging instrumentation on board the spacecraft Dynamics Explorer 1 (DE 1) is used to observe the large-scale motion of a surge over 7000 km along the auroral oval from near local midnight. Average speed of the surge is 2.2 km/s. Ground-based observations at Fort Yukon, Alaska, show the classical looped, multiple-arc structure of a westward traveling surge as it passes overhead. Within the 6-min temporal resolution provided with DE 1, the surge advances initially at a speed of about 8 km/s followed by a steady decline to about 1 km/s over a period of 17 min. This sequence is then repeated a second time, beginning with a significant intensification of the surge form. This intense surge activity is not accompanied by significant auroral activity near magnetic midnight. Following passage of the surge, persistent and localized bright emission regions remain along the auroral oval for several tens of minutes. Average separation distances are approximately 700 km. If these persistent features identify the sites of individual stepwise advances of the surge, the average time per advance is about 5 min.

Craven, J. D.↗
NASA NTRS2011

A Super-Resolution Algorithm for Enhancement of FLASH LIDAR data

A novel method for enhancement of the spatial resolution of 3-dimensional Flash Lidar images is being proposed for generation of elevation maps of terrain from a moving platform. NASA recognizes the Flash LIDAR technology as an important tool for enabling safe and precision landing in future unmanned and crewed lunar and planetary missions. The ability of the Flash LIDAR to generate 3-dimensional maps of the landing site area during the final stages of the descent phase for detection of hazardous terrain features such as craters, rocks, and steep slopes is under study in the frame of the Autonomous Landing and Hazard Avoidance (ALHAT) project. Since single frames of existing FLASH LIDAR systems are not sufficient to build a map of entire landing site with acceptable spatial resolution and precision, a super-resolution approach utilizing multiple frames has been developed to overcome the instrument s limitations. Performance of the super-resolution algorithm has been analyzed through a series of simulation runs obtained from a high fidelity Flash LIDAR model and a high resolution synthetic lunar elevation map. For each simulation run, a sequence of FLASH LIDAR frames are recorded and processed as the spacecraft descends toward the landing site. Simulations runs having different trajectory profiles and varying LIDAR look angles of the terrain are also analyzed. The results show that adequate levels of accuracy and precision are achieved for detecting hazardous terrain features and identifying safe areas of the landing site.

Bulyshev, Alexander↗
DOE OSTI2020

High-resolution Spectra of Earth-like Planets Orbiting Red Giant Host Stars

In the near future we will have ground- and space-based telescopes that are designed to observe and characterize Earth-like planets. While attention is focused on exoplanets orbiting main-sequence stars, more than 150 exoplanets have already been detected orbiting red giants, opening the intriguing question of what rocky worlds orbiting in the habitable zone (HZ) of red giants would be like and how to characterize them. We present a high-resolution spectral database of reflection and emission spectra for nominal Earth-like planets orbiting in the red giant HZ from the visible to infrared (0.4–20 μm) for planets orbiting at the Earth-equivalent distance. We also show the change of such planetary spectra through the evolution of their red giant hosts. While the luminosity of the host increases the contrast ratio between star and planet, the increased orbital distance of the HZ for red giant hosts relaxes the light suppression requirements close to the star, which could make such planets interesting targets to characterize and search for signs of life, if new coronagraph designs with higher suppression at larger orbital separations could be developed. We assess the feasibility of characterizing atmospheric features including biosignatures for such planets with the proposed mission concept Large UV/Optical/IR Surveyor (LUVOIR).

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1995

Diverse Thermus species inhabit a single hot spring microbial mat

Through an effort to characterize aerobic chemoorganotrophic bacteria in the Octopus Spring cyano-bacterial mat community, we cultivated four Thermus isolates with unique 16S rRNA sequences. Isolates clustered within existing Thermus clades, including those containing Thermus ruber, Thermus aquaticus, and a subgroup closely related to T. aquaticus. One Octopus Spring isolate is nearly identical (99.9% similar) to isolates from Iceland, and two others are closely related to a T. ruber isolated from Russia. Octopus Spring isolates similar to T. aquaticus and T. ruber exhibited optimal growth rates at high (65-70 degrees C) and low (50 degrees C) temperatures, respectively, with the most abundant species best adapted to the temperature of the habitat (50-55 degrees C). Our results display a diversity of Thermus genotypes defined by 16S rRNA within one hot spring microbial community. We suggest that specialization to temperature and perhaps other local environmental features controls the abundance of Thermus populations.

Non-NASA Center↗