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At least 973 records · Page 54
All sourcesNASA NTRSDOE OSTI
DOE OSTI2018

SOST/Sclerostin Improves Posttraumatic Osteoarthritis and Inhibits MMP2/3 Expression After Injury: SOST OVEREXPRESSION IMPROVES PTOA OUTCOMES

Patients with anterior cruciate ligament (ACL) rupture are two times as likely to develop posttraumatic osteoarthritis (PTOA). Annually, there are ~900,000 knee injuries in the United States, which account for ~12% of all osteoarthritis (OA) cases. PTOA leads to reduced physical activity, deconditioning of the musculoskeletal system, and in severe cases requires joint replacement to restore function. Therefore, treatments that would prevent cartilage degradation post-injury would provide attractive alternatives to surgery. Sclerostin (Sost), a Wnt antagonist and a potent negative regulator of bone formation, has recently been implicated in regulating chondrocyte function in OA. To determine whether elevated levels of Sost play a protective role in PTOA, we examined the progression of OA using a noninvasive tibial compression overload model in SOST transgenic (SOST TG ) and knockout (Sost -/- ) mice. Here we report that SOST TG mice develop moderate OA and display significantly less advanced PTOA phenotype at 16 weeks post-injury compared with wild-type (WT) controls and Sost -/- . In addition, SOST TG built ~50% and ~65% less osteophyte volume than WT and Sost -/- , respectively. Quantification of metalloproteinase (MMP) activity showed that SOSTTG had ~2-fold less MMP activation than WT or Sost-/-, and this was supported by a significant reduction in MMP2/3 protein levels, suggesting that elevated levels of SOST inhibit the activity of proteolytic enzymes known to degrade articular cartilage matrix. Furthermore, intra-articular administration of recombinant Sost protein, immediately post-injury, also significantly decreased MMP activity levels relative to PBS-treated controls, and Sost activation in response to injury was TNFα and NF-κB dependent. These results provide in vivo evidence that sclerostin functions as a protective molecule immediately after joint injury to prevent cartilage degradation.

Endocrinology & Metabolism↗
DOE OSTI2020

Tomography of the positive-pitch fast-ion velocity distribution in DIII-D plasmas with Alfvén eigenmodes and neoclassical tearing modes

Understanding the effect of Alfvén eigenmodes (AEs) and neoclassical tearing modes (NTMs) on fast ions is highly important for fusion reactors due to potentially strong resonant interactions between the fast ions and the modes. Here, we use the four-view fast-ion D-alpha (FIDA) diagnostic installed in the DIII-D tokamak to reconstruct the fast-ion velocity distribution at two radial positions during two sequential discharges with strong and weak mode activity, respectively. The velocity-space coverage of the diagnostics, however, only allows reliable reconstructions of fast ions with positive pitches. Therefore, we suggest new tomographic inversion methods relying on prior information outside the well-diagnosed region. We find that within the population of fast ions with positive pitches, ions, at all energies, are transported away from the measurement volumes. Comparisons between the reconstructions and kick model simulations, where the mode activity is considered, reveal that low-frequency modes such as the NTMs and low-frequency AEs contribute significantly to the positive-pitch fast-ion transport in the central measurement volume, whereas TAEs and EAEs become important farther out and are responsible for decreased fast-ion confinement.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2020

Hybrid Sorbents for 129 I Capture from Contaminated Groundwater

Radioiodine ( 129 I) poses a risk to the environment due to its long half-life, toxicity, and mobility. It is found at the U.S. Department of Energy Hanford Site due to legacy releases of nuclear wastes to the subsurface where 129 I is predominantly present as iodate (IO 3 – ). To date, a cost-effective and scalable cleanup technology for 129 I has not been identified, with hydraulic containment implemented as the remedial approach. Here, novel high-performing sorbents for 129 I remediation with the capacity to reduce 129 I concentrations to or below the US Environmental Protection Agency (EPA) drinking water standard and procedures to deploy them in an ex-situ pump and treat (P&T) system are introduced. Furthermore, this includes implementation of hybridized polyacrylonitrile (PAN) beads for ex-situ remediation of IO 3 – -contaminated groundwater for the first time. Iron (Fe) oxyhydroxide and bismuth (Bi) oxyhydroxide sorbents were deployed on silica substrates or encapsulated in porous PAN beads. In addition, Fe–, cerium (Ce)–, and Bi–oxyhydroxides were encapsulated with anion-exchange resins. The PAN–bismuth oxyhydroxide and PAN–ferrihydrite composites along with Fe- and Ce-based hybrid anion-exchange resins performed well in batch sorption experiments with distribution coefficients for IO 3 – of >1000 mL/g and rapid removal kinetics. Of the tested materials, the Ce-based hybrid anion-exchange resin was the most efficient for removal of IO 3 – from Hanford groundwater in a column system, with 50% breakthrough occurring at 324 pore volumes. The functional amine groups on the parent resin and amount of active sorbent in the resin can be customized to improve the iodine loading capacity. These results highlight the potential for IO 3 – remediation by hybrid sorbents and represent a benchmark for the implementation of commercially available materials to meet EPA standards for cleanup of 129 I in a large-scale P&T system.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS1995

Carbonic Anhydrase is Required for Statoconia Homeostasis in Organ Cultures of Statocysts from Aplysia californica

A novel organ culture system has been developed to study the regulation of statoconia production in the gravity sensing organ in Aplysia californica. Statocysts were cultured in Leibovitz (LI5) medium supplemented with salts and Aplysia haemolymph for four days at 17 C. The viability of the system was evaluated by examining four parameters: statocyst morphology, the activity of the mechanosensory cilia in the statocyst, production of new statoconia during culture and change in statoconia volume after culture. There were no morphological differences in statocysts before and after culture when ciliary beating was maintained. There was a 29% increase in the number of statoconia after four days in culture. Mean statocyst, statolith and statoconia volumes were not affected by culture conditions. The presence of carbonic anhydrase in the statocysts was shown using immunohistochemistry. When statocysts were cultured in the presence of 4.0 x 10(exp -4) M acetazolamide to inhibit the enzyme activity, there was a decrease in statoconia production and statoconia volume, indicating a role for this enzyme in statoconia homeostasis, potentially, via pH regulation. These studies are the first to report a novel system for the culture of statocysts and show that carbonic anhydrase is involved in the regulation of statoconia volume and production.

Pedrozo, H. A.↗
NASA NTRS1998

Use of an adaptable cell culture kit for performing lymphocyte and monocyte cell cultures in microgravity

The results of experiments performed in recent years on board facilities such as the Space Shuttle/Spacelab have demonstrated that many cell systems, ranging from simple bacteria to mammalian cells, are sensitive to the microgravity environment, suggesting gravity affects fundamental cellular processes. However, performing well-controlled experiments aboard spacecraft offers unique challenges to the cell biologist. Although systems such as the European 'Biorack' provide generic experiment facilities including an incubator, on-board 1-g reference centrifuge, and contained area for manipulations, the experimenter must still establish a system for performing cell culture experiments that is compatible with the constraints of spaceflight. Two different cell culture kits developed by the French Space Agency, CNES, were recently used to perform a series of experiments during four flights of the 'Biorack' facility aboard the Space Shuttle. The first unit, Generic Cell Activation Kit 1 (GCAK-1), contains six separate culture units per cassette, each consisting of a culture chamber, activator chamber, filtration system (permitting separation of cells from supernatant in-flight), injection port, and supernatant collection chamber. The second unit (GCAK-2) also contains six separate culture units, including a culture, activator, and fixation chambers. Both hardware units permit relatively complex cell culture manipulations without extensive use of spacecraft resources (crew time, volume, mass, power), or the need for excessive safety measures. Possible operations include stimulation of cultures with activators, separation of cells from supernatant, fixation/lysis, manipulation of radiolabelled reagents, and medium exchange. Investigations performed aboard the Space Shuttle in six different experiments used Jurkat, purified T-cells or U937 cells, the results of which are reported separately. We report here the behaviour of Jurkat and U937 cells in the GCAK hardware in ground-based investigations simulating the conditions expected in the flight experiment. Several parameters including cell concentration, time between cell loading and activation, and storage temperature on cell survival were examined to characterise cell response and optimise the experiments to be flown aboard the Space Shuttle. Results indicate that the objectives of the experiments could be met with delays up to 5 days between cell loading into the hardware and initial in flight experiment activation, without the need for medium exchange. Experiment hardware of this kind, which is adaptable to a wide range of cell types and can be easily interfaced to different spacecraft facilities, offers the possibility for a wide range of experimenters successfully and easily to utilise future flight opportunities.

short duration↗
DOE OSTI2013

Acceleration of bone development and regeneration through the Wnt/β-catenin signaling pathway in mice heterozygously deficient for GSK-3β

Highlights: •The Wnt/β-catenin signaling pathway was activated in GSK-3β{sup +/−} mice. •The cortical and trabecular bone volumes were increased in GSK-3β{sup +/−} mice. •Regeneration of a partial bone defect was accelerated in GSK-3β{sup +/−} mice. -- Abstract: Glycogen synthase kinase (GSK)-3β plays an important role in osteoblastogenesis by regulating the Wnt/β-catenin signaling pathway. Therefore, we investigated whether GSK-3β deficiency affects bone development and regeneration using mice heterozygously deficient for GSK-3β (GSK-3β{sup +/−}). The amounts of β-catenin, c-Myc, cyclin D1, and runt-related transcription factor-2 (Runx2) in the bone marrow cells of GSK-3β{sup +/−} mice were significantly increased compared with those of wild-type mice, indicating that Wnt/β-catenin signals were enhanced in GSK-3β{sup +/−} mice. Microcomputed tomography of the distal femoral metaphyses demonstrated that the volumes of both the cortical and trabecular bones were increased in GSK-3β{sup +/−} mice compared with those in wild-type mice. Subsequently, to investigate the effect of GSK-3β deficiency on bone regeneration, we established a partial bone defect in the femur and observed new bone at 14 days after surgery. The volume and mineral density of the new bone were significantly higher in GSK-3β{sup +/−} mice than those in wild-type mice. These results suggest that bone formation and regeneration in vivo are accelerated by inhibition of GSK-3β, probably through activation of the Wnt/β-catenin signaling pathway.

60 APPLIED LIFE SCIENCES↗
NASA NTRS2017

The Challenge Posed by Geomagnetic Activity to Electric Power Reliability: Evidence from England and Wales

This paper addresses whether geomagnetic activity challenged the reliability of the electric power system during part of the declining phase of solar cycle 23. Operations by National Grid in England and Wales are examined over the period of 11 March 2003 through 31 March 2005. This paper examines the relationship between measures of geomagnetic activity and a metric of challenged electric power reliability known as the net imbalance volume (NIV). Measured in megawatt hours, NIV represents the sum of all energy deployments initiated by the system operator to balance the electric power system. The relationship between geomagnetic activity and NIV is assessed using a multivariate econometric model. The model was estimated using half-hour settlement data over the period of 11 March 2003 through 31 December 2004. The results indicate that geomagnetic activity had a demonstrable effect on NIV over the sample period. Based on the parameter estimates, out-of-sample predictions of NIV were generated for each half hour over the period of 1 January to 31 March 2005. Consistent with the existence of a causal relationship between geomagnetic activity and the electricity market imbalance, the root-mean-square error of the out-of-sample predictions of NIV is smaller; that is, the predictions are more accurate, when the statistically significant estimated effects of geomagnetic activity are included as drivers in the predictions.

Forbes, Kevin F.↗
NASA NTRS2005

Thermal Emission Variability of Zamama, Culann and Tupan on Io Using Galileo Near-Infrared Mapping Spectrometer (NIMS) Data

The Jovian satellite Io is the most volcanically active body in the Solar System. Previous analyses [e.g., 1-4] indicate the presence of high-temperature silicate volcanism on Io, similar to silicate volcanism occurring on Earth. Instruments onboard the Galileo spacecraft, especially the Near Infrared Mapping Spectrometer (NIMS) and the Solid State Imager (SSI), provided much data of Io s active volcanoes throughout the duration of the Galileo mission (June 1996-September 2003). NIMS data is particularly sensitive to thermal emission from active and cooling lava over cooling times of seconds to a few years. The objective of this ongoing study of Io s volcanism is to determine the variability of thermal emission from volcanoes on Io s surface, in order to better understand the styles of eruption, and to constrain the volumes of material erupted. Ultimately, this will help to constrain the contribution of active volcanism to Io s thermal budget. Data have been analyzed for the volcano Zamama, located at 173 W, 21 N, and the power output of Zamama, the volumes of lava being erupted, and the eruption rate determined. Culann and Tupan have also been analysed in this way. This abstract primarily concentrates on Zamama.

Ennis, M. E.↗
NASA NTRS1984

Cometary nucleus and active regions

On the basis of the icy conglomerate model of cometary nuclei, various observations demonstrate the spotted nature of many or most nuclei, i.e., regions of unusual activity, either high or low. Rotation periods, spin axes and even precession of the axes are determined. The observational evidence for variations in activity over the surfaces of cometary nuclei are listed and discussed. On June 11 the comet IRAS-ARAKI-ALCOCK approached the Earth to a distance of 0.031 AU, the nearest since C/Lexell, 1770 I, providing a unique opportunity for near-nucleus observations. Preliminary analysis of these images establishes the spin axis of the nucleus, with an oblioquity to the orbit plane of approximately 50 deg, and a lag angle of sublimation approximately 35 deg from the solar meridian on the nucleus. Asymmetries of the inner coma suggests a crazy-quilt distribution of ices with differing volatility over the surface of the nucleus. The observations of Comet P/Homes 1892 III, exhibiting two 8-10 magnitude bursts, are carefully analyzed. The grazing encounter produced, besides the first great burst, an active area on the nucleus, which was rotating retrograde with a period of 16.3hr and inclination nearly 180 deg. After the first burst the total magnitude fell less than two magnitudes from November 7 to November 30 (barely naked eye) while the nuclear region remained diffuse or complex, rarely if ever showing a stellar appearance. The fading was much more rapid after the second burst. The grazing encounter distributed a volume of large chunks in the neighborhood of the nucleus, maintaining activity for weeks.

Whipple, F. L.↗
NASA NTRS1984

The study of the physics of cometary nuclei

The observations of comet P/Holmes 1892III, exhibiting two 8 to 10 magnitude bursts, were carefully analyzed. The phenomena are consistent with the grazing encounter of a small satellite with the nucleus. The grazing encounter produced, besides the first great burst, an active area on the nucleus, which was rotating retrograde with a period of 16.3 hr and inclination nearly 180 deg. After the final encounter, the spin period was essentially unchanged, but two areas became active, separated some 164 deg in longitude on the nucleus. After the first burst the total magnitude fell less than two magnitudes, while the nuclear region remained diffuse or complex, rarely if ever showing a stellar appearance. The fading was much more rapid after the second burst (barely naked eye at maximum) while the nucleus frequently stellar after the first day. It seems reasonable to conclude that the grazing encounter distributed a volume of large chunks in the neighborhood of the nucleus, maintaining activity for weeks.

Whipple, F. L.↗
DOE OSTI2013

Determination of Radioisotope Content by Measurement of Waste Package Dose Rates - 13394

The objective of this communication is to report the observed correlation between the calculated air kerma rates produced by radioactive waste drums containing untreated ion-exchange resin and activated charcoal slurries with the measured radiation field of each package. Air kerma rates at different distances from the drum surface were calculated with the activity concentrations previously determined by gamma spectrometry of waste samples and the estimated mass, volume and geometry of solid and liquid phases of each waste package. The water content of each waste drum varies widely between different packages. Results will allow determining the total activity of wastes and are intended to complete the previous steps taken to characterize the radioisotope content of wastes packages. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2015

Tritiated waste management opportunities based on the reduction of tritium activity and outgassing - 15607

Tritiated waste is called as such because tritium is the main radionuclide contained in this waste which is generally produced by nuclear research, fusion facilities and small producers, e.g. hospitals, research centers and industries. The management of this type of waste can be improved by using techniques that reduce the tritium activity and outgassing levels. Several options are possible depending on the waste form and the amount of tritium: temporary storage for tritium decay up to 50 years, thermal treatment such as incineration and melting, and high-integrity containers for conditioning, either considered alone or in combination with one of the other options. This paper compares a number of waste management parameters with respect to tritiated waste: the environmental impact of tritium releases, secondary waste management, volume reduction of the waste for final disposal, and the waste activity after treatment, not to mention the related safety issues, the performance of the techniques used in each option, and economic aspects. The results of this comparative work are given in this paper, with the advantages and drawbacks of each option discussed in detail. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
NASA NTRS1998

Human heart rate variability relation is unchanged during motion sickness

In a study of 18 human subjects, we applied a new technique, estimation of the transfer function between instantaneous lung volume (ILV) and instantaneous heart rate (HR), to assess autonomic activity during motion sickness. Two control recordings of ILV and electrocardiogram (ECG) were made prior to the development of motion sickness. During the first, subjects were seated motionless, and during the second they were seated rotating sinusoidally about an earth vertical axis. Subjects then wore prism goggles that reverse the left-right visual field and performed manual tasks until they developed moderate motion sickness. Finally, ILV and ECG were recorded while subjects maintained a relatively constant level of sickness by intermittent eye closure during rotation with the goggles. Based on analyses of ILV to HR transfer functions from the three conditions, we were unable to demonstrate a change in autonomic control of heart rate due to rotation alone or due to motion sickness. These findings do not support the notion that moderate motion sickness is manifested as a generalized autonomic response.

NASA Discipline Neuroscience↗
NASA NTRS2002

Physiological changes, sleep, and morning mood in an isolated environment

BACKGROUND: Previous isolation studies have shown increased 24-h urine volumes and body weight gains in subjects. This project examined those and other physiological variables in relationship to sleep motor activity, subjective sleep quality, mood, and complaints during confinement. METHODS: Six male and two female subjects lived for 7 d in the National Space Development Agency of Japan's isolation chamber, which simulates the interior of the Japanese Experiment Module. Each 24-h period included 6 h of sleep, 3 meals, and 20 min of exercise. Each morning, subjects completed Sleep Sensation and Complaint Index questionnaires. Catecholamine and creatinine excretion, urine volume, and body weight were measured on the 2 d before and 2 d after confinement, and sleep motor activity was measured during confinement. RESULTS: Confinement produced no significant change in body weight, urine volume, or questionnaire results. In contrast, epinephrine, norepinephrine, and sleep motor activity exhibited significant differences during confinement (p < 0.05). Higher nocturnal norepinephrine excretion correlated with higher sleep motor activity. CONCLUSION: The 24-h epinephrine values were slightly higher than normal throughout the experiment, but lower than for subjects working under time-stress. High sympathetic activity (as indicated by norepinephrine) may have interfered with sleep.

NASA Discipline Space Human Factors↗
DOE OSTI2021

Deployment of the Finite Volume Method in Pronghorn for Gas and Salt cooled Pebble Bed Reactors

This report summarizes the activities related to ”Complete FHR and HTGR pebble bed simulator, including initial validation” funded by the NEAMS thermal-hydraulics focus area. The activity revolves around the coarse-mesh thermal-hydraulics code Pronghorn and its application to gas and salt cooled Pebble bed reactor (PBR). The main difference between gas and salt cooled PBR from a thermal-hydraulics perspective is the fluid. To address the difference in fluid behavior, two separate approaches are implemented in the MOOSE Navier Stokes module: 1) a Boussinesq approximation and 2) a fully compressible formulation. The developed finite volume method capabilities are used for improving pre-existing gas-cooled and salt-cooled pebble-bed reactor models. A steady-state, multiphysics gas-cooled pebble-bed reactor model is created that couples the equilibrium core depletion capability developed in previous work, and the finite volume method capability developed for this report. The salt-cooled pebble-bed reactor model is upgraded to use the incompressible finite volume method capability and then extended to three spatial dimensions. Finally, several verification-and-validation exercises performed with Pronghorn are documented using the verification-and-validation report of the MooseDoc system. The goal of this effort to document the verification-and-validation level of Pronghorn and improve stakeholder confidence in the results obtained with Pronghorn.

97 MATHEMATICS AND COMPUTING↗
DOE OSTI2018

CO 2 storage in depleted oil and gas fields in the Gulf of Mexico

Depleted oil and gas reservoirs are one of the prime-candidate formations for geologic CO 2 storage. Although both the geological structure and the physical properties of most of them have been extensively studied and characterized, there is limited data on the assessment of the CO 2 storage capacity, especially in the offshore fields. The purpose of this study is to develop a high-level quantitative assessment of the CO 2 volume that can be stored in depleted oil and gas fields in the Federal offshore regions of the Gulf of Mexico (GOM), both on a field-by-field and on a reservoir-by-reservoir basis. In this study, we simulated CO 2 storage in 461 of the depleted oil and gas reservoirs (73 fields) among 3514 reservoirs (675 fields) in the GOM (2013 BOEM Reserves database). Based on the simulation results, we improved the Department of Energy (DOE) CO 2 Storage Resource Estimate Equation to make more refined and accurate estimates of storable CO 2 volumes. Newly revised efficiency factor (ERoil/gas) correlates better with hydrocarbon recovery factor (HCRF), which is found to be a strong indicator of the CO 2 storage capacity of the reservoir. The higher HCRF results in higher ERoil/gas. The further investigations resulted in an improved, material balance-based correlation—which is called the Production-CO 2 Storage Correlation—between cumulative production (free gas, oil and water) at reservoir conditions and CO 2 storage volume at standard conditions. This relationship, which is unique for all three types of reservoirs (gas, oil and combination), allows for making direct estimates of CO 2 storage volume using only existing production data. Application of these correlations to all of the depleted fields (3514 reservoirs) yields CO 2 storage capacities of 4748 MMtons, and the CO 2 storage capacity in all 1295 depleted and active fields (13,289 reservoirs) in the GOM calculated to be 21.57 Billion tons. If a 5000 psia surface injection pressure constraint was applied, these volumes would be reduced to 4075 MMtons for all depleted fields only and to 15.80 Billion tons for all depleted and active fields in the GOM. Therefore, the production-CO 2 storage correlations can be used to make more accurate CO 2 storage volume estimates in all onshore and offshore depleted oil and gas fields.

03 NATURAL GAS↗
DOE OSTI2014

SU-E-T-546: Use of Implant Volume for Quality Assurance of Low Dose Rate Brachytherapy Treatment Plans

Purpose: To analyze the application of volume implant (V100) data as a method for a global check of low dose rate (LDR) brachytherapy plans. Methods: Treatment plans for 335 consecutive patients undergoing permanent seed implants for prostate cancer and for 113 patients treated with plaque therapy for ocular melanoma were analyzed. Plaques used were 54 COMS (10 to 20 mm, notched and regular) and 59 Eye Physics EP917s with variable loading. Plots of treatment time x implanted activity per unit dose versus v100 ^.667 were made. V100 values were obtained using dose volume histograms calculated by the treatment planning systems (Variseed 8.02 and Plaque Simulator 5.4). Four different physicists were involved in planning the prostate seed cases; two physicists for the eye plaques. Results: Since the time and dose for the prostate cases did not vary, a plot of implanted activity vs V100 ^.667 was made. A linear fit with no intercept had an r{sup 2} = 0.978; more than 94% of the actual activities fell within 5% of the activities calculated from the linear fit. The greatest deviations were in cases where the implant volumes were large (> 100 cc). Both COMS and EP917 plaque linear fits were good (r{sup 2} = .967 and .957); the largest deviations were seen for large volumes. Conclusions: The method outlined here is effective for checking planning consistency and quality assurance of two types of LDR brachytherapy treatment plans (temporary and permanent). A spreadsheet for the calculations enables a quick check of the plan in situations were time is short (e.g. OR-based prostate planning)

07 ISOTOPE AND RADIATION SOURCES↗
NASA NTRS2000

Cardiovascular response to lower body negative pressure stimulation before, during, and after space flight

BACKGROUND: It is well known that space travel cause post-flight orthostatic hypotension and it was assumed that autonomic cardiovascular control deteriorates in space. Lower body negative pressure (LBNP) was used to assess autonomic function of the cardiovascular system. METHODS: LBNP tests were performed on six crew-members before and on the first days post-flight in a series of three space missions. Additionally, two of the subjects performed LBNP tests in-flight. LBNP mimics fluid distribution of upright posture in a gravity independent way. It causes an artificial sequestration of blood, reduces preload, and filtrates plasma into the lower part of the body. Fluid distribution was assessed by bioelectrical impedance and anthropometric measurements. RESULTS: Heart rate, blood pressure, and total peripheral resistance increased significantly during LBNP experiments in-flight. The decrease in stroke volume, the increased pooling of blood, and the increased filtration of plasma into the lower limbs during LBNP indicated that a plasma volume reduction and a deficit of the interstitial volume of lower limbs rather than a change in cardiovascular control was responsible for the in-flight response. Post-flight LBNP showed no signs of cardiovascular deterioration. The still more pronounced haemodynamic changes during LBNP reflected the expected behaviour of cardiovascular control faced with less intravascular volume. In-flight, the status of an intra-and extravascular fluid deficit increases sympathetic activity, the release of vasoactive substances and consequently blood pressure. Post-flight, blood pressure decreases significantly below pre-flight values after restoration of volume deficits. CONCLUSION: We conclude that the cardiovascular changes in-flight are a consequence of a fluid deficit rather than a consequence of changes in autonomic signal processing.

manned↗