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Biomolecule Sequencer: Next-Generation DNA Sequencing Technology for In-Flight Environmental Monitoring, Research, and Beyond

On the International Space Station (ISS), technologies capable of rapid microbial identification and disease diagnostics are not currently available. NASA still relies upon sample return for comprehensive, molecular-based sample characterization. Next-generation DNA sequencing is a powerful approach for identifying microorganisms in air, water, and surfaces onboard spacecraft. The Biomolecule Sequencer payload, manifested to SpaceX-9 and scheduled on the Increment 4748 research plan (June 2016), will assess the functionality of a commercially-available next-generation DNA sequencer in the microgravity environment of ISS. The MinION device from Oxford Nanopore Technologies (Oxford, UK) measures picoamp changes in electrical current dependent on nucleotide sequences of the DNA strand migrating through nanopores in the system. The hardware is exceptionally small (9.5 x 3.2 x 1.6 cm), lightweight (120 grams), and powered only by a USB connection. For the ISS technology demonstration, the Biomolecule Sequencer will be powered by a Microsoft Surface Pro3. Ground-prepared samples containing lambda bacteriophage, Escherichia coli, and mouse genomic DNA, will be launched and stored frozen on the ISS until experiment initiation. Immediately prior to sequencing, a crew member will collect and thaw frozen DNA samples, connect the sequencer to the Surface Pro3, inject thawed samples into a MinION flow cell, and initiate sequencing. At the completion of the sequencing run, data will be downlinked for ground analysis. Identical, synchronous ground controls will be used for data comparisons to determine sequencer functionality, run-time sequence, current dynamics, and overall accuracy. We will present our latest results from the ISS flight experiment the first time DNA has ever been sequenced in space and discuss the many potential applications of the Biomolecule Sequencer for environmental monitoring, medical diagnostics, higher fidelity and more adaptable Space Biology Human Research Program investigations, and even life detection experiments for astrobiology missions.

Sequencer↗

NASA’s Identified Risks of Adverse Outcomes Due to Inadequate Human Systems Integration Architecture in Human Spaceflight

The NASA Human System Risk Board (HSRB) has the overall responsibility for tracking the evolution of the top ~30 human system risks that it has identified to be associated with human spaceflight. As part of this process, the Board is charged with maintaining a consistent, integrated process to mitigate those risks, and developing evidence-based risk posture recommendations. One of the identified risks is due to inadequate human systems integration architecture (HSIA) and a driving factor of this risk is that given decreasing real-time ground support for execution of complex operations during future exploration missions, there is a possibility of adverse performance outcomes including that crew are unable to adequately respond to unanticipated critical malfunctions or detect safety critical procedural errors. The HSRB uses Directed Acyclic Graphs (DAGs) as a communication tool for describing how astronaut exposure to spaceflight hazards leads to meaningful mission-level health and performance outcomes and as the basis for understanding intermediate causal relationships between risk contributing factors and countermeasures that link hazards to outcomes. The HSIA risk DAG will be presented and described. Historically, critical malfunctions requiring Crew/MCC management occurred at a rate of 1.7 times per year for ISS averaged over the lifetime and 3-4 times per year in the burn in phase for the vehicle. These averages do not include EVA data, which greatly increases the incident rate. Prior experience from the Apollo program showed 10/11 crewed missions experienced significant anomalies where crew relied heavily on MCC expertise in real-time. These failure patterns are in line with those observed in other complex engineered systems (e.g., oil rigs, launch systems, commercial aviation, etc.) It is likely that general malfunction and error rates are > 10% for short duration missions (<30 days), based on past and current spaceflight operations data. Likelihood of adverse outcomes has the potential to increase as crew conduct work with new, complex systems and with less ground support. For Low Earth Orbit missions and Lunar missions less than 30 days, assuming minimal comm delays, disruptions and bandwidth limitations, malfunctions and errors can affect mission objectives and crew health but may be mitigated by ground support. For Lunar missions greater than 30 days and any potential Mars mission malfunctions and errors can have Loss of Crew and Loss of Mission consequences due to reduced ground support (communication delays, constraints and blackouts) for more complex operations, as well as reduced resupply and evacuation options.

Daniel M Buckland↗

NASA X-HAB Water Delivery System

As part of the eXploration Systems and Habitation (X-HAB) Academic Innovation Challenge program of National Aeronautics and Space Administration (NASA), The Ohio State University was selected to improve the performance of NASA’s existing Vegetable Production System (VEGGIE), which is a deployable plant growth unit for International Space Station (ISS). During the academic year 2015-2016, The Ohio State University student team developed a passive water delivery system using capillary water transport principle (Jenson et al. 2016). The major design improvement made was directly connecting the water reservoir to the plant-rooting pillows using a single-interface capillary cord design. Harvestable plants were successfully grown from seeds using the single interface system. In addition, Nomex®, a fabric material composed of short nylon based fibers, was identified as the material for wicks. Finally, the water reservoir was modeled as a propellant management device (PMD) to ensure consistent and long term watering of the VEGGIE system. The PMDs are made of materials that utilize surface tension and adhesive forces to improve stability and fluid delivery. The team recommended using a sponge PMD in order to mitigate bubble obstruction, decrease system weight, and ensure reliable water delivery to the capillary interface.

Peter Ling↗

Gumstix ISS Camera

With many jobs that are considered to be too meticulous for the astronauts on the International Space Station, our commercial camera aims to eliminate some of those tasks. Debris and other metal are often found falling away from the station, and this could signal a repair to be made. These cameras, programmed with OpenCV motion detection software, would be able to recognize such debris and relay a live feed to operators either on the station or back on Earth.

Pacheco, Natali↗

Magik Animation of Robotic Operations for Flight SpaceX-29 to ISS

The MAGIK Robotic analysis team provides kinematic feasibility assessments for the ISS and has produced an animation to evaluate Extravehicular Robotics (EVR) activities for the SpaceX (SpX)-29 flight. The animation also displays the current ISS configuration at the time of the flight. Flight SpX-29 includes a SpaceX Cargo Dragon vehicle docked to Node 2 Forward (N2F) International Docking Adapter (IDA) and consists of the delivery of AWE (Atmospheric Waves Experiment) and Integrated LCRD LEO User Modem Amplifier optical communications Terminal (ILLUMA-T). Robotic operations shown include the Special Purpose Dexterous Manipulator (SPDM) Trunk extraction of ILLUMA-T and maneuvering to the JEM RMS Handoff position, the SPDM Trunk extraction of AWE, maneuvering to Express Logistics Carrier (ELC) 1 Site 3 to remove Space Test Program - Houston 5 (STP-H5), and the installation of AWE to ELC-1 Site 3. There is no disposal payload for this flight.

EVR↗

A Technology Demonstration Experiment for Laser Cooled Atomic Clocks in Space

We have been developing a laser-cooling apparatus for flight on the International Space Station (ISS), with the intention of demonstrating linewidths on the cesium clock transition narrower than can be realized on the ground. GLACE (the Glovebox Laser- cooled Atomic Clock Experiment) is scheduled for launch on Utilization Flight 3 (UF3) in 2002, and will be mounted in one of the ISS Glovebox platforms for an anticipated 2-3 week run. Separate flight definition projects funded at NIST and Yale by the Micro- gravity Research Division of NASA as a part of its Laser Cooling and Atomic Physics (LCAP) program will follow GLACE. Core technologies for these and other LCAP missions are being developed at JPL, with the current emphasis on developing components such as the laser and optics subsystem, and non-magnetic vacuum-compatible mechanical shutters. Significant technical challenges in developing a space qualifiable laser cooling apparatus include reducing the volume, mass, and power requirements, while increasing the ruggedness and reliability in order to both withstand typical launch conditions and achieve several months of unattended operation. This work was performed at the Jet Propulsion Laboratory under a contract with the National Aeronautics and Space Administration.

Klipstein, W. M.↗

Remote Operations and Ground Control Centers

The Payload Operations Integration Center at the Marshall Space Flight Center supports the International Space Station (ISS) through remote interfaces around the world. The POI$ was originally designed as a gateway to space for remote facilities; ranging from an individual user to a fullscale multi-user environment. This achievement was accomplished while meeting program requirements and accommodating the injection of modem technology on an ongoing basis to ensure cost operations. This paper will discuss the open POIC architecture developed to support similar and dissimilar remote operations centers. It will include technologies, protocols, and compromises which on a day to day basis support ongoing operations. Additional areas covered include centralized management of shared resources and methods utilized to provide highly available and restricted resources to remote users. Finally, the effort of coordinating the actions of participants will be discussed.

Bryant, Barry S.↗

Remote Operations and Ground Control Centers

The Payload Operations Integration Center (POIC) at the Marshall Space Flight Center supports the International Space Station (ISS) through remote interfaces around the world. The POIC was originally designed as a gateway to space for remote facilities; ranging from an individual user to a full-scale multiuser environment. This achievement was accomplished while meeting program requirements and accommodating the injection of modern technology on an ongoing basis to ensure cost effective operations. This paper will discuss the open POIC architecture developed to support similar and dissimilar remote operations centers. It will include technologies, protocols, and compromises which on a day to day basis support ongoing operations. Additional areas covered include centralized management of shared resources and methods utilized to provide highly available and restricted resources to remote users. Finally, the effort of coordinating the actions of participants will be discussed.

Bryant, Barry S.↗

Growing Beyond Earth: Student Citizen Science Contributing to Space Crop Production

Fairchild Tropical Botanic Garden and NASA have been partnering since 2015 to conduct a citizen science education program for middle and high school students called Growing Beyond Earth (GBE). Growing Beyond Earth is a multi-classroom science project designed to advance NASA’s research on growing plants in space. GBE was implemented locally and scaled nationally under two NASA Grants. Now serving more than 250 schools and over 10,000 middle and high school students nationwide, GBE successfully improved STEM education. It also contributed student-generated data to NASA, improving NASA research on the ground and on ISS, with two student-selected crops grown in space. GBE is unique in its focus on real scientific research, enabling student “citizen scientists” to contribute data toward NASA mission planning. Each classroom receives a Fairchild-designed plant habitat analogous to the plant growing equipment aboard the International Space Station (ISS). Fairchild and NASA scientists train teachers to conduct in-classroom GBE experiments, and students then share experimental data online with NASA. As NASA looks toward a long-term human presence beyond Earth’s orbit, there are specific science, technology, engineering, and math challenges related to food production in space. During this presentation, learn how GBE is addressing those challenges by expanding the diversity and quality of edible plants that can be grown aboard spacecraft. We will share the significant scientific and educational results that have come out of this partnership and explain how we quickly pivoted to allow students to continue to contribute during the COVID-era. Finally, we will explain how on Earth, GBE is also advancing technologies for growing plants in urban, indoor, and other resource-limited settings through the GBE Maker challenge for High School, University, and Professional communities of Makers across the country to develop the next generation of space crop production technologies. These programs are supported by NASA.

Growing Beyond Earth↗

Acquisition of Long-Duration, Low-Gravity Slosh Data Utilizing Existing ISS Equipment (SPHERES) for Calibration of CFD Models of Coupled Fluid-Vehicle Behavior

Accurate prediction of coupled fluid slosh and launch vehicle or spacecraft dynamics (e.g., nutation/precessional movement about various axes, attitude changes, ect.) requires Computational Fluid Dynamics (CFD) models calibrated with low-gravity, long duration slosh data. Recently completed investigations of reduced gravity slosh behavior have demonstrated the limitations of utilizing parabolic flights on specialized aircraft with respect to the specific objectives of the experiments. Although valuable data was collected, the benefits of longer duration low-gravity environments were clearly established. The proposed research provides the first data set from long duration tests in zero gravity that can be directly used to benchmark CFD models, including the interaction between the sloshing fluid and the tank/vehicle dynamics. To explore the coupling of liquid slosh with the motion of an unconstrained tank in microgravity, NASA's Kennedy Space Center, Launch Services Program has teamed up with the Florida Institute of Technology (FIT), Massachusetts Institute of Technology (MIT) and the Office of the Chief Technologist (OCT) to perform a series of slosh dynamics experiments on the International Space Station using the SPHERES platform. The Synchronized Position Hold Engage Reorient Experimental Satellites (SPHERES) testbed provides a unique, free-floating instrumented platform on ISS that can be utilized in a manner that would solve many of the limitations of the current knowledge related to propellant slosh dynamics on launch vehicle and spacecraft fuel tanks. The six degree of freedom (6-DOF) motion of the SPHERES free-flyer is controlled by an array of cold-flow C02 thrusters, supplied from a built-in liquid C02 tank. These SPHERES can independently navigate and re-orient themselves within the ISS. The intent of this project is to design an externally mounted tank to be driven inside the ISS by a set of two SPHERES devices (Figure 1 ). The tank geometry simulates a launch vehicle upper stage propellant tank and the maneuvers replicate those of real vehicles. The design includes inertial sensors, data acquisition, image capture and data storage interfaces to the SPHERES VERTIGO computer system on board the flight article assembly. The design also includes mechanical and electronic interfaces to the existing SPHERES hardware, which include self-contained packages that can operate in conjunction with the existing SPHERES electronics.

Schallhorn, Paul↗

NASA HRP Food and Nutrition Risk Strategy

The Human Health Countermeasures (HHC) Element of NASA’s Human Research Program (HRP), along with other organizations within NASA, conduct research and capability developments to buy down the risk of performance decrement and crew illness due to inadequate food and nutrition. Food and nutrition are critical for health and performance both in low Earth orbit and on exploration missions. On the International Space Station (ISS), crewmembers share a defined set of pantry foods, and have limited preference and shelf-life foods (e.g. fruits and vegetables) from resupply missions. Exploration design reference missions (DRMs) will likely be more constrained than missions to ISS, where those crewmembers would be subject to a more restricted food system due to resource limitations. HRP food and nutrition research consists of studies to close the following research gaps: determination of the nutritional requirements to maintain health and performance for the defined DRMs, determining the nutrient content, safety, and acceptability of the spaceflight food system specific to DRM and vehicle constraints, development of countermeasures either within or in addition to the food system to mitigate decrements to health and performance by DRM, and the validation and integration of food system countermeasures in analogs and in flight. The current spaceflight food system is shelf-stable, consisting of freeze-dried foods, thermostabilized foods, natural form foods, and powdered beverages. Crops represent a food system countermeasure that has the potential to supplement the pre-packaged food system. As an applied research program, HRP goals for crop research are centered around three main themes: nutrition, safety, and acceptability. As crops advance through Crop Readiness Level evaluations, HHC partners with the sponsoring organization to ensure that HRP goals are met for crops to be consumed in spaceflight.

food↗

Solid Inflammability Boundary At Low-Speed (SIBAL)

This research program is concerned with the effect of low-speed flow on the spreading and extinction processes over solid fuels. The project has passed the Science Concept Review and the experiment is currently scheduled to be performed in the ISS Combustion Integrated Rack. We present an overview of recent and ongoing experimental and theoretical efforts.

T'ien, J.↗

STS-114: Discovery Day 3/Mission Status Briefing

Paul Hill, STS-114 Lead Flight Director, and John Shannon, Flight Operations and Integrations Manager for the Space Shuttle Program were present. Paul gave a detailed description of the Orbiter's performance upon its arrival on the International Space Station, orbital rendezvous and docking was completed, performance was nominal by all measures, and crew is already inside the ISS. He also briefly mentioned the next day crew activities, robotics work and first space walk. John emphasized on ground technical engineering tasks, data gathering and inspection of data, imagery and damage assessment, assessing the performance of the external tank, engineering analysis, and the increase of understanding of the overall condition of the vehicle. Safety of the vehicle, battery lifetime, foam loss, tile damage, post launch analysis were some of the topics discussed with the News Media.

Source record↗

Fatigue‐Related Countermeasures for Long‐Duration Exploration Missions

The NASA Human Research Program's (HRP) Behavioral Health and Performance Element (BHP) supports and conducts research to mitigate deleterious outcomes related to fatigue, sleep loss, circadian desynchronization, and work overload. Objective evidence indicates that within the context of the International Space Station (ISS), sleep is reduced and there is circadian misalignment. Despite chronic sleep loss and high workloads; however, astronauts successfully complete their missions. Contributing to their success is not only the tremendous skills and capabilities of each astronaut, but also the collaborative team efforts amongst the crew, between flight and ground crews, and through real‐time care provided by medical personnel. It is anticipated that risks to human health and performance will increase in the context of exploration missions, where crewmembers will venture to deep space for extended durations and in small vehicles with limited communication with home. Hence, fatigue‐related countermeasures are being developed and/or validated that include unobtrusive monitoring technologies to detect fatigue‐related performance decrements, environmental countermeasures, and sleep education and training for flight and ground crews. Given that fatigue is an issue in current ISS missions, the BHP works collaboratively with Space Medicine operations to collect data in the operational environment, to validate fatigue-related countermeasures, and provide evidence‐based mitigations. Our presentation will summarize fatigue‐related operational research that is underway through NASA's BHP in partnership with its operational counterparts. Efforts include studies evaluating the effects of hypnotics, lighting protocols as countermeasures for circadian entrainment, and investigations involving education and training. This presentation will further identify, based on flight and terrestrial evidence, additional sleep and circadian countermeasures that may still be needed to support exploration missions. Lessons learned from transitioning research deliverables into ISS operations will also be discussed.

Whitmire, A.↗

Quantitative Comparison of Proprietary and Open-Source Georeferencing Tools for Use with Astronaut Photography

The Crew Earth Observations (CEO) Facility within the Earth Science and Remote Sensing Unit at NASA’s Johnson Space Center supports the acquisition, analysis, and curation of astronaut photography of Earth’s surface and atmosphere. Astronauts on the International Space Station (ISS) respond to requests from CEO to acquire imagery of scientific and education targets, to include high profile targets in response to activations from the International Charter for Space & Major Disasters (also known as the International Disaster Charter, or IDC) and NASA’s Disasters Program. CEO facilitates the acquisition of astronaut photography in response to IDC events and delivers georeferenced data products to the United States Geological Survey (USGS) for distribution to the disaster community. Using GeoRef, an internal web-based tool developed in collaboration with NASA’s Ames Research Center, CEO generates data packages of georeferenced imagery, uncertainty images for assessing control and tie point accuracy, and metadata documenting raw and processed data. Operational experience with the Georef software identified vulnerabilities to internal code and server errors that can significantly increase time of data production. As such, CEO developed a backup procedure in case the GeoRef software experiences front-end or back-end errors. A system using OSGEO’s open-source QGIS software combined with a semi-automated pipeline using the object-oriented Python language and the Geospatial Abstract Library for generating metadata is quantitatively compared to GeoRef’s data package for quality and productivity. Root Mean Square Error (RMSE) provides a standard measurement of data quality as it relates to ground error. Assessing RMSE measurements generated from georeferenced astronaut photographs acquired with different obliquity and focal length offers a comprehensive accuracy assessment of the software’s transformation algorithms. This assessment will indicate the software's ability to produce data products with the least ground-error or highest data quality regarding ground accuracy. In addition, a comparison of the software’s efficiency in generating a data package that includes georeferenced images, metadata, and uncertainty images for measuring tie/ground point error was performed. Initial results, based on the comparison of three nadir-facing astronaut photographs acquired with a 95mm focal length, reveal the QGIS-based system's average RMSE is 2.36 (pixels) suggesting its georectification system produces data products that meet and perhaps improve upon Georef solution's average RMSE of 32.99 (pixels). However, the QGIS system was unable to reproduce two unique Georef data products, uncertainty images for measuring tie and control point errors and a translated unwrapped image. In addition, the Georef software is designed to accept handheld camera pose information from a hardware component (Geosens) scheduled for deployment on the ISS in late 2018; this information is intended to provide increased accuracy and auto-registration capability for astronaut photographs. Future work is expected to determine the QGIS-based georectification system’s potential as an open-source alternative (and operational backup) to Georef for georeferencing the full range of resolutions and viewing angles unique to handheld digital camera imagery in support of ISS disaster response activities.

Jagge, Amy M.↗

Behavioral Health Program Element

The project goal is to develop behavioral health prevention and maintenance system for continued crew health, safety, and performance for exploration missions. The basic scope includes a) Operationally-relevant research related to clinical cognitive and behavioral health of crewmembers; b) Ground-based studies using analog environments (Antarctic, NEEMO, simulations, and other testbeds; c) ISS studies (ISSMP) focusing on operational issues related to behavioral health outcomes and standards; d) Technology development activities for monitoring and diagnostic tools; and e) Cross-disciplinary research (e.g., human factors and habitability research, skeletal muscle, radiation).

Leveton, Lauren B.↗

Safety Aboard the International Space Station

As with any task that NASA takes on, safety is of utmost importaqce. There are pages of safety codes and procedures that must be followed before any idea can be brought to life. Unfortunately, the International Space Station s (ISS) safety regulations and procedures are based on lg standards rather than on Og. To aide in making this space age home away from home a less hazardous environment, I worked on several projects revolving around the dangers of flammable items in microgravity. The first task I was assigned was to track flames. This involves turning eight millimeter video recordings, of tests run in the five second drop tower, into avi format on the computer. The footage is then compressed and altered so that the flame can be seen more clearly. Using another program called Spotlight, line profiles were used to collect data describing the luminescence of the flame at different points. These raw data are saved as text files and run trough a macro so that a Matlab program can analyze it. By fitting the data to a curve and determining the areas of brightest luminescence, the behavior of the flame can be recorded numerically. After entering the data into a database, researchers can come back later and easily get information on flames resulting from different gas and liquid mixtures in microgravity. I also worked on phase two of the FATE project, which deals with safety aboard the ISS. This phase involves igniting projected droplets and determining how they react with secondary materials. Such simulations represent, on a small scale, the spread of onboard fires due to the effervescence of burning primary materials. I set up existing hardware to operate these experiments and ran tests with it, photographing the results. I also made CAD drawings of the apparatus and the area available on the (SF)2 rig for it to fit into. The experiment will later be performed on the KC-135, and the results gathered will be used to reanalyze current safety standards for the ISS, including the distance of required separation for flammable materials. Additional information is included in the original extended abstract.

Mintz, Shauna M.↗

Research Progress and Accomplishments on ISS

The first research payloads reached the International Space Station (ISS) more than two years ago, with research operating continuously since March 2001. Seven research racks are currently on-orbit, with three more arriving soon to expand science capabilities. Through the first five expeditions, 60 unique NASA-managed investigations from 11 nations have been supported, many continuing into later missions. More than 90,000 experiment hours have been completed, and more than 1,000 hours of crew time have been dedicated to research, numbers that grow daily. The multidisciplinary program includes research in life sciences, physical sciences, biotechnology, Earth sciences, technology demonstrations as well as commercial endeavors and educational activities. The Payload Operations and Integration Center monitors the onboard activities around the clock, working with numerous Principal Investigators and Payload Developers at their remote sites. Future years will see expansion of the station with research modules provided by the European Space Agency and Japan, which will be outfitted with additional research racks. The first research payloads arrived at ISS more than two years ago, and continuous science has been ongoing for more than one and a half years. During this time, the research capabilities have been tremendously increased, even as assembly of the overall platform continues. Despite significant challenges along the way, ISS continues to successfully support a large number of investigations in a variety of research disciplines. The results of some of the early investigations are reaching the publication stage. The near future looms with new challenges, but experience to date and dedicated efforts give reason to be optimistic that the challenges will be overcome and that new and greater successes will be added to past ones.

Roe, Lesa B.↗