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At least 1,045 records · Page 58

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DOE OSTI2024

Enhancing Biopreparedness through a Model System to Understand the Molecular Mechanisms that Lead to Pathogenesis and Disease Transmission: NW-BRaVE

The science of biopreparedness to counter biological threats hinges on understanding the fundamental principles and molecular mechanisms that lead to pathogenesis and disease transmission. Our vision to address this challenge is to create a powerful and user-friendly platform to elucidate the fundamental principles of how molecular interactions drive pathogen-host relationships and host shifts. We will enable groundbreaking discoveries by integrating a wide range of structural, genomics, proteomics, and other advanced omics measurements, along with evolutionary and artificial intelligence predictions. To make sure the system is applicable to real-world problems, we will develop it in the context of a tractable model system, the small, abundant, and accessible photosynthetic cyanobacteria and their constantly co-adapting viral pathogens, cyanophages. This model will maintain the system’s applicability to real-world problems and techniques, but the overall focus will be on elucidating general principles of detecting, assessing, and surveilling molecular interaction, adaptation, and coevolution that are system agnostic and therefore extensible to other viral-host interactions. Our overall objectives are to (1) identify the molecular complexes that comprise the cyanobacteria redox macromolecular subsystem and how they dynamically change with bacteriophage infection in situ, using cryo-electron tomography; (2) profile regulatory changes during infection using proteomics, multiomics, and experimental validation, and integrate the data with in situ structures; (3) use genomics and metagenomics to determine environmental and population factors across time scales that impact the interactions between marine cyanobacteria and their cyanophage parasites, predicting the evolutionary origins of in situ structural and functional interactions, convergence and coevolution; and (4) develop a data integration and transformation platform that facilitates the integration of in situ, proteomic, and evolutionary measurements of molecular interactions to surveil diverse hosts and parasites in various environmental contexts. These objectives address Focus Area 2 Reveal Molecular Interactions Across Biological Scales for Design of Targeted Interventions. Our powerful and user-friendly platform will enhance connections between the often-siloed fields of structure, molecular phenotype, and evolutionary genomics that are key to biopreparedness, but in need of integration (Figure 1). We will build an integrated navigation tool to facilitate the effective use of globally distributed experimental data for integrated analysis and predictive modeling. The project will develop, implement, and test a platform to assess host-pathogen molecular interactions, adaptation to hosts and host shifts, and coevolution between hosts and pathogens, successfully impacting the research community by revolutionizing abilities to study any host-pathogen interaction, encourage diverse community contributions, and gain fundamental insights into how proteins adapt to new contexts. This ability will be critical for designing early interventions to address future threats. We will build surveillance training capability, aiming for a fair and equitable response to future pandemics and biothreats.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2026

A Simulator for Neyer Tests of Explosives

Explosives and explosive devices such as detonators are typically tested by applying a range of stimuli such as voltage or mechanical shock, and recording binary “detonated/did not detonate” responses. These are analyzed using maximum likelihood or generalized linear models to provide estimates of quantities such as the all-fire and no-fire points. Given that the true threshold for detonation is unknown a priori , sequential design methods are typically used to optimize the set of test points. One popular method, implemented in commercial software, is Neyer’s algorithm. To support simulation and experimental design, we have developed code in the R programming language to duplicate the functions of the Neyer software. We provide code for the simulator along with a description and examples of usage.

42 ENGINEERING↗

DOE OSTI2024

Thermochemical Nonequilibrium Modeling in a Continuous-Galerkin, Finite-Element Framework

The presented work discusses the implementation, verification, and validation of Park's two-temperature model in a scalable, computational fluid dynamics (CFD) code developed at the US Department of Energy's Oak Ridge National Laboratory (ORNL). The implementation of Park's two-temperature model was verified through 0D test cases involving an adiabatic reactor and a nitrogen thermal bath. The implementation was then validated through comparisons with other validated CFD codes and experimental data on a hypersonic cylinder and double cones. These are standard benchmark test cases for thermochemical non-equilibrium (TCNE) modeling, and all data are shared publicly. The verification and validation results showed that ORNL's in-house CFD code could model complex, high-speed flow problems with and without TCNE modeling. This work is essential for future research involving 3D shock wave/boundary layer interactions (SBLIs).

Nutter, Nicole↗

NASA NTRS1973

Evolution of the satellite telemetry data processing facility at the Goddard Space Flight Center.

Data from scientific and application satellites managed by the NASA Goddard Space Flight Center are acquired through the world-wide Spacecraft Tracking and Data Network (STDN). These data are forwarded to a central telemetry data processing facility whose primary objective is the timely provision of the data to users in a form suitable for their analysis. In a successful evolution, a satellite data processing facility must adapt to changing support requirements while operationally supporting active spacecraft. Advances in the technology of data transmission, data storage systems, and file management make it feasible to implement a third-generation system which will be able to satisfy experimenter requirements for the next ten years.

Keipert, F. A.↗

NASA NTRS1982

Flying qualities criteria for GA single pilot IFR operations

The flying qualities criteria in general aviation (GA) to decrease accidents are discussed. The following in-flight research is discussed: (1) identification of key aerodynamic configurations; (2) implementation of an in-flight simulator; (3) mission matrix design; (4) experimental systems; (5) data reduction; (6) optimal flight path reconstruction. Some of the accomplished work is reported: an integrated flight testing and flight path reconstruction methodology was developd, high accuracy in trajectory estimation was achieved with an experimental setup, and a part of the flight test series was flown.

Bar-Gill, A.↗

NASA NTRS1988

Characterization of fatigue crack initiation and propagation in Ti-6Al-4V with electrical potential drop technique

Electrical potential methods have been used in the past primarily to monitor crack length in long crack specimens subjected to fatigue loading. An attempt was made to develop test procedures for monitoring the fatigue crack initiation and the growth of short fatigue cracks in a turbine disk alloy with the electrical potential drop technique (EPDT). In addition, the EPDT was also applied to monitor the fatigue crack growth in long crack specimens of the same alloy. The resolution of the EPDT for different specimen geometries was determined. Factors influencing the EPDT are identified and the applicability of EPDT in implementing damage tolerant design concepts for turbine disk materials is discussed. The experimental procedure adopted and the results obtained is discussed. No substantial differences were observed between the fatigue crack growth data of short and long crack specimens.

Kalluri, Sreeramesh↗

NASA NTRS1988

Experimental studies in system identification of helicopter rotor dynamics

Recent experiments investigating the system identification of helicopter rotor dynamics are described. The identification makes use of a two-pass procedure that estimates the rotor dynamic states prior to estimation of the dynamic equation parameters. Estimation of the rotor states is made possible through use of the predictive information contained in blade-mounted accelerometers combined with a specialized processing scheme utilizing these signals. Descriptions of the experimental hardware and the system identification technique are given, as well as implementation issues for using the procedure on other similarly instrumented rotor blades. Finally, comparisons with other identification techniques using the same data are presented. It is demonstrated that the approach is an attractive one for measurement of a helicopter rotor's dynamic behavior.

Mckillip, Robert, Jr.↗

NASA NTRS1988

Recent laboratory results of the KITE attitude dynamics simulator

It has previously been shown that, for all but the most simple tethered satellite missions, conventional means of performing attitude control are insufficient. A particularly effective method of implementing attitude control for tethered satellites is to use the tether tension force to generate control torques by moving the tether attach point relative to the satellite center of mass. It has been proposed to demonstrate this attitude control technique in a Space Shuttle flight test project known as the Kinetic Isolation Tether Experiment (KITE). This paper describes a scaled, one dimensional laboratory simulation of the KITE mission. The simulator has been built to verify theoretical predictions of attitude control ability and to investigate the technological requirements in order to implement this concept. The laboratory apparatus is described in detail and preliminary experimental results are presented and discussed. The results to date have shown a fine pointing accuracy of 5 arc-seconds RMS and a closed-loop bandwidth of 0.08 Hz.

Kline-Schoder, Robert↗

NASA NTRS1991

Resolution of a target-tracking optical novelty filter

The resolution of a target-tracking optical novelty filter is discussed in terms of the response time of the nonlinear medium, the speed of the target, and the resolution of the input device. Optical novelty filters using a faster nonlinear medium may have a higher output resolution. This is particularly true in the case of tracking high-speed targets. The potential of implementing high-resolution optical novelty filters using photorefractive GaAs is investigated experimentally.

Liu, Duncan T. H.↗

NASA NTRS1992

An overview of the NASA Advanced Propulsion Concepts program

NASA Advanced Propulsion Concepts (APC) program for the development of long-term space propulsion system schemes is managed by both NASA-Lewis and the JPL and is tasked with the identification and conceptual development of high-risk/high-payoff configurations. Both theoretical and experimental investigations have been undertaken in technology areas deemed essential to the implementation of candidate concepts. These APC candidates encompass very high energy density chemical propulsion systems, advanced electric propulsion systems, and an antiproton-catalyzed nuclear propulsion concept. A development status evaluation is presented for these systems.

Curran, Francis M.↗

NASA NTRS1993

A layered approach to structural control system design for the JPL phase B testbed

This paper describes our efforts in structural control experiments for a flexible precision structure using both passive and active control. Specifically, a layered structural control approach utilizing passive viscous dampers, colocated and noncolocated active control is used. The passive dampers and active colocated control loops are used to enhance stability and robustness and the noncolocated multivariable controller is designed with respect to a partially controlled structure to further improve the performance. Our approach to the multivariable design problem requires not only a state space model description of the plant, but also an accompanying uncertainty model. To support the multivariable robust control design development, a significant effort in system identification was undertaken. The process of identification and identified results are discussed. Noncolocated controllers are designed using the H(infinity)/mu-synthesis methodology. The order of the controller is typically large, hence, model reduction is performed for practical digital implementation on a real-time control system developed at JPL. Finally, experimental results are presented and some of lessons are discussed.

Chu, Cheng-Chih↗

NASA NTRS1989

CSI compensation for reduced-order model based control of a flexible robot manipulator

In controller design for flexible structures, certain system modes are extremely important for the overall performance of the structure. A reduced-order model (ROM) based control focuses on these modes, providing a viable, active control algorithm for large systems. Unfortunately, unmodeled structure dynamics can interact with the ROM controller (CSI) and cause crippling deterioration of system performance, possibly to the point that system stability is lost. A residual model filter (RMF) eliminates one channel of control structure interaction (CSI), while adding only a simple, second-order filter to the control loop. Thus, the ROM controller can be designed independently, based strictly on performance criteria, and residual mode filters can then be selected to compensate for CSI. A flexible robot manipulator is used for preliminary experimentation with the ROM/RMF design methodology. Since the controller was to be implemented both with, and without compensation for CSI, the ROM control gains are carefully chosen such that closed loop stability is never compromised. In this way, RMF effectiveness is easily evaluated in terms of the improvement in system performance resulting from CSI compensation.

Reisenauer, Brian T.↗

NASA NTRS1993

Active control of buildings during earthquakes

The objective of this report is to provide an overview of the different types of control systems used in buildings, to discuss the problems associated with current active control mechanisms, and to show the cost-effectiveness of applying active control to buildings. In addition, a small case study investigates the feasibility and benefits of using embedded actuators in buildings. Use of embedded actuators could solve many of the current problems associated with active control by providing a wider bandwidth of control, quicker speed of response, increased reliability and reduced power requirement. Though embedded actuators have not been developed for buildings, they have previously been used in space structures. Many similarities exist between large civil and aerospace structures indicating that direct transfer of concepts between the two disciplines may be possible. In particular, much of the Controls-Structures Interaction (CSI) technology currently being developed could be beneficially applied to civil structures. While several buildings with active control systems have been constructed in Japan, additional research and experimental verification are necessary before active control systems become widely accepted and implemented.

Vance, Vicki L.↗

NASA NTRS1994

The Buffer Diagnostic Prototype: A fault isolation application using CLIPS

This paper describes problem domain characteristics and development experiences from using CLIPS 6.0 in a proof-of-concept troubleshooting application called the Buffer Diagnostic Prototype. The problem domain is a large digital communications subsystems called the real-time network (RTN), which was designed to upgrade the launch processing system used for shuttle support at KSC. The RTN enables up to 255 computers to share 50,000 data points with millisecond response times. The RTN's extensive built-in test capability but lack of any automatic fault isolation capability presents a unique opportunity for a diagnostic expert system application. The Buffer Diagnostic Prototype addresses RTN diagnosis with a multiple strategy approach. A novel technique called 'faulty causality' employs inexact qualitative models to process test results. Experimental knowledge provides a capability to recognize symptom-fault associations. The implementation utilizes rule-based and procedural programming techniques, including a goal-directed control structure and simple text-based generic user interface that may be reusable for other rapid prototyping applications. Although limited in scope, this project demonstrates a diagnostic approach that may be adapted to troubleshoot a broad range of equipment.

Porter, Ken↗

NASA NTRS1996

Adjustment of Sensor Locations During Thermal Property Parameter Estimation

The temperature dependent thermal properties of a material may be evaluated from transient temperature histories using nonlinear parameter estimation techniques. The usual approach is to minimize the sum of the squared errors between measured and calculated temperatures at specific locations in the body. Temperature measurements are usually made with thermocouples and it is customary to take thermocouple locations as known and fixed during parameter estimation computations. In fact, thermocouple locations are never known exactly. Location errors on the order of the thermocouple wire diameter are intrinsic to most common instrumentation procedures (e.g., inserting a thermocouple into a drilled hole) and additional errors can be expected for delicate materials, difficult installations, large thermocouple beads, etc.. Thermocouple location errors are especially significant when estimating thermal properties of low diffusively materials which can sustain large temperature gradients during testing. In the present work, a parameter estimation formulation is presented which allows for the direct inclusion of thermocouple positions into the primary parameter estimation procedure. It is straightforward to set bounds on thermocouple locations which exclude non-physical locations and are consistent with installation tolerances. Furthermore, bounds may be tightened to an extent consistent with any independent verification of thermocouple location, such as x-raying, and so the procedure is entirely consonant with experimental information. A mathematical outline of the procedure is given and its implementation is illustrated through numerical examples characteristic of light-weight, high-temperature ceramic insulation during transient heating. The efficacy and the errors associated with the procedure are discussed.

Milos, Frank S.↗

NASA NTRS2003

Optimal Limited Contingency Planning

For a given problem, the optimal Markov policy over a finite horizon is a conditional plan containing a potentially large number of branches. However, there are applications where it is desirable to strictly limit the number of decision points and branches in a plan. This raises the question of how one goes about finding optimal plans containing only a limited number of branches. In this paper, we present an any-time algorithm for optimal k-contingency planning. It is the first optimal algorithm for limited contingency planning that is not an explicit enumeration of possible contingent plans. By modelling the problem as a partially observable Markov decision process, it implements the Bellman optimality principle and prunes the solution space. We present experimental results of applying this algorithm to some simple test cases.

Meuleau, Nicolas↗

NASA NTRS2008

TEM Cell Testing of Cable Noise Reduction Techniques From 2 MHz to 200 MHz - Part 1

This paper presents empirical results of cable noise reduction techniques as demonstrated in a TEM cell operating with radiated fields from 2 - 200 MHz. It is the first part of a two-paper series. This first paper discusses cable types and shield connections. In the second paper, the effects of load and source resistances and chassis connections are examined. For each topic, well established theories are compared to data from a real-world physical system. Finally, recommendations for minimizing cable susceptibility (and thus cable emissions) are presented. There are numerous papers and textbooks that present theoretical analyses of cable noise reduction techniques. However, empirical data is often targeted to low frequencies (e.g. <50 KHz) or high frequencies (>100 MHz). Additionally, a comprehensive study showing the relative effects of various noise reduction techniques is needed. These include the use of dedicated return wires, twisted wiring, cable shielding, shield connections, changing load or source impedances, and implementing load- or source-to-chassis isolation. We have created an experimental setup that emulates a real-world electrical system, while still allowing us to independently vary a host of parameters. The goal of the experiment was to determine the relative effectiveness of various noise reduction techniques when the cable is in the presence of radiated emissions from 2 MHz to 200 MHz. The electronic system (Fig. 1) consisted of two Hammond shielded electrical enclosures, one containing the source resistance, and the other containing the load resistance. The boxes were mounted on a large aluminium plate acting as the chassis. Cables connecting the two boxes measured 81 cm in length and were attached to the boxes using standard D38999 military-style connectors. The test setup is shown in Fig. 2. Electromagnetic fields were created using an HP8657B signal generator, MiniCircuits ZHL-42W-SMA amplifier, and an EMCO 5103 TEM cell. Measurements were taken using an Agilent E4401B spectrum analyzer and HP1141a differential probes.

Bradley, Arthur T.↗

NASA NTRS2008

TEM Cell Testing of Cable Noise Reduction Techniques from 2 MHz to 200 MHz -- Part 2

This paper presents empirical results of cable noise reduction techniques as demonstrated in a TEM cell operating with radiated fields from 2 - 200 MHz. It is the second part of a two-paper series. The first paper discussed cable types and shield connections. In this second paper, the effects of load and source resistances and chassis connections are examined. For each topic, well established theories are compared to data from a real-world physical system. Finally, recommendations for minimizing cable susceptibility (and thus cable emissions) are presented. There are numerous papers and textbooks that present theoretical analyses of cable noise reduction techniques. However, empirical data is often targeted to low frequencies (e.g. <50 KHz) or high frequencies (>100 MHz). Additionally, a comprehensive study showing the relative effects of various noise reduction techniques is needed. These include the use of dedicated return wires, twisted wiring, cable shielding, shield connections, changing load or source impedances, and implementing load- or source-to-chassis isolation. We have created an experimental setup that emulates a real-world electrical system, while still allowing us to independently vary a host of parameters. The goal of the experiment was to determine the relative effectiveness of various noise reduction techniques when the cable is in the presence of radiated emissions from 2 MHz to 200 MHz.

Bradley, Arthur T.↗