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At least 1,063 records · Page 59

9 Li($\textit{d,p}$) reaction as a specific probe of 10 Li, the paradigm of parity-inverted nuclei around the $\textit{N}$ = 6 closed shell

In this work, we show, within the framework of renormalized nuclear field theory and of the induced reaction surrogate formalism, that the highly debated 10 Li structure, studied in a recent high statistics 9 Li ($\textit{d,p}$) 10 Li one-neutron transfer experiment, is consistent with, or better, requires, the presence of a virtual 1/2 + state of similar single-particle strength than that of the 1/2 – resonance at 0.45 ± 0.03 MeV. Based on continuum spectroscopy self-energy techniques, we find that the physical mechanism responsible for parity inversion in $^{10}_3$Li is the same as that at the basis of the similar phenomenon observed in $^{11}_4$Be and as that needed in 11 Li to have an important $\textit{s}$-wave ground-state component. In particular the strong dynamical coupling between the $s_{1/2}$ and the $d_{5/2}$ states, mediated by the quadrupole vibration of the core 9 Li. A phenomenon which also affects the strength distribution of the $d_{5/2}$ state, in particular, in the energy range of 3–4.5 MeV. Furthermore, this mechanism is also consistent with the (normal) sequence of the $1_{p_{1/2}}$ and $2_{s_{1/2}}$ levels in the $\textit{N}$ = 7 isotones $^{12}_5$B and $^{13}_6$C. The main aim of the present Rapid Communication is that of treating structure and reactions on equal footing and in a common language. In other words, the calculation of the 9 Li ($\textit{d,p}$) 10 Li reaction as a single conceptual step from individual single-particle motion and collective vibrations to absolute double differential cross sections of renormalized virtual and resonant final states, which can be directly compared with experiment.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

APOGEE Net: Improving the Derived Spectral Parameters for Young Stars through Deep Learning

Machine learning allows for efficient extraction of physical properties from stellar spectra that have been obtained by large surveys. The viability of machine-learning approaches has been demonstrated for spectra covering a variety of wavelengths and spectral resolutions, but most often for main-sequence (MS) or evolved stars, where reliable synthetic spectra provide labels and data for training. Spectral models of young stellar objects (YSOs) and low-mass MS stars are less well-matched to their empirical counterparts, however, posing barriers to previous approaches to classify spectra of such stars. In this work, we generate labels for YSOs and low-mass MS stars through their photometry. We then use these labels to train a deep convolutional neural network to predict logg, T {sub eff}, and Fe/H for stars with Apache Point Observatory Galactic Evolution Experiment (APOGEE) spectra in the DR14 data set. This “APOGEE Net” has produced reliable predictions of logg for YSOs, with uncertainties of within 0.1 dex and a good agreement with the structure indicated by pre-MS evolutionary tracks, and it correlates well with independently derived stellar radii. These values will be useful for studying pre-MS stellar populations to accurately diagnose membership and ages.

79 ASTRONOMY AND ASTROPHYSICS↗

Transition of Site Programs and Implementation of Decommissioning Agent Oversight - 18653

San Onofre Nuclear Generating Station (SONGS), acting as the Decommissioning Agent (DA), retained its NRC License and contracted a Decommissioning General Contractor (DGC) to manage plant programs and execute the Demolition and Dismantlement (D and D) of the site. To support this effort, over twenty-one programs transitioned to the DGC, including Occupational Health and Safety, Radiation Protection, Maintenance, and Engineering. In addition, SONGS effected a cultural shift from an operating station performing work to one of overseeing it. The framework and road map for this kind of D and D model is new to the industry. This presentation shares the SONGS' experience and lessons learned associated with this transition. SONGS and the DGC entered an Agreement that established the contractual requirements and responsibilities of the parties, the Scope of Work, and the mechanisms to ensure effective transition of DA programs. As a part of defining the Scope of Work, the contract established functional areas and divisions of responsibilities divided into the roles of Governance, Oversight, Support, and Performance (GOSP). SONGS, as the site licensee, retained the Oversight Role, with exception of those areas that, as the licensee, would be considered 'non-transferrable'. Examples of those programs were Quality Assurance, Regulatory Affairs and the Employee Concerns Program. While the Company retained responsibility for those programs the DGC developed their own 'parallel' programs that the Company provided Oversight for. In 2017, SONGS completed program transition within one-year following the notice-to-proceed. The overall strategy was driven by three high-level principles: - Allow the DGC the most unfettered execution of the Scope of Work with limited interference and maximum self-governance. With the overriding principle that transitioned programs have a direct link to a basis in regulation or law. - Identify safety and compliance risks, and then bridge the transition of programs from the SONGS to the DGC such that identified risks are minimized and/or mitigated. - Allow the SONGS organization to transition from a Performance role to a primarily Oversight role as the DA. Working collaboratively with the DGC, programs were strategically grouped and sequenced for turnover. Central to the success of transition was fostering a collaborative environment with the DGC, facilitating knowledge transfer, applying schedule rigor, and keeping the team horizontally and vertically aligned to management expectations. SONGS achieved this alignment by embedding the formal process with management expectations for review and acceptance of proposed programs. SONGS' transitioned core programs to a general contractor and transition from a 'perform' organization to an 'oversight' organization. Lessons learned from this experience may benefit other nuclear generating station owners currently evaluating options for site D and D. (author)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Camelina sativa , an oilseed at the nexus between model system and commercial crop

The rapid assessment of metabolic engineering strategies in plants is aided by crops that provide simple, high throughput transformation systems, a sequenced genome, and the ability to evaluate the resulting plants in field trials. Camelina sativa provides all of these attributes in a robust oilseed platform. The ability to perform field evaluation of Camelina is a useful, and in some studies essential benefit that allows researchers to evaluate how traits perform outside the strictly controlled conditions of a greenhouse. In the field the plants are subjected to higher light intensities, seasonal diurnal variations in temperature and light, competition for nutrients, and watering regimes dictated by natural weather patterns, all which may affect trait performance. There are difficulties associated with the use of Camelina . The current genetic resources available for Camelina pale in comparison to those developed for the model plant Arabidopsis thaliana; however, the sequence similarity of the Arabidopsis and Camelina genomes often allows the use of Arabidopsis as a reference when additional information is needed. Camelina ’s genome, an allohexaploid, is more complex than other model crops, but the diploid inheritance of its three subgenomes is straightforward. The need to navigate three copies of each gene in genome editing or mutagenesis experiments adds some complexity but also provides advantages for gene dosage experiments. In conclusion, the ability to quickly engineer Camelina with novel traits, advance generations, and bulk up homozygous lines for small-scale field tests in less than a year, in our opinion, far outweighs the complexities associated with the crop.

59 BASIC BIOLOGICAL SCIENCES↗

Global identification of circular RNAs in chronic myeloid leukemia reveals hsa{sub c}irc{sub 0}080145 regulates cell proliferation by sponging miR-29b

Highlights: • A large number of circRNAs aberrantly expressed in chronic myeloid leukemia. • Top 20 aberrantly expressed interacted with leukemia-associated miRNAs. • hsa{sub c}irc{sub 0}080145 knockdown significantly suppressed CML cell proliferation. • hsa{sub c}irc{sub 0}080145 acts as an miR-29b sponge in CML. Circular RNAs (circRNAs) are a class of non-coding RNAs that participate in various biological processes and disease pathogenesis. However, the role of circRNAs in chronic myeloid leukemia (CML) remains largely unknown. In this study, high-throughput sequencing was performed to explore the expression profile of circRNAs in CML patients for the first time, and a large number of differentially expressed circRNAs were identified. In addition, we constructed potential circRNA-miRNA interaction networks in CML, including a detailed hsa{sub c}irc{sub 0}080145-mediated competing endogenous RNA (ceRNA) regulatory network. Furthermore, functional experiments demonstrated that hsa{sub c}irc{sub 0}080145 knockdown significantly suppressed CML cell proliferation and hsa{sub c}irc{sub 0}080145 regulated CML cell proliferation by acting as an miR-29b sponge.

60 APPLIED LIFE SCIENCES↗

QCD hidden-color hexadiquark in the core of nuclei

Hidden-color configurations are a key prediction of QCD with important physical consequences. In this work we examine a QCD color-singlet configuration in nuclei formed by combining six scalar [ud] diquarks in a strongly bound SU (3) C channel. The resulting hexadiquark state is a charge-2, spin-0, baryon number-4, isospin-0, color-singlet state. It contributes to alpha clustering in light nuclei and to the additional binding energy not saturated by ordinary nuclear forces in as well as the alpha-nuclei sequence of interest for nuclear astrophysics. We show that the strongly bound combination of six scalar isospin-0 [ud] diquarks within the nuclear wave function - relative to free nucleons - provides a natural explanation of the EMC effect measured by the CLAS collaboration's comparison of nuclear parton distribution function ratios for a large range of nuclei. Overall, these experiments confirmed that the EMC effect; i.e., the distortion of quark distributions within nuclei, is dominantly identified with the dynamics of neutron-proton (“isophobic”) short-range correlations within the nuclear wave function rather than proton-proton or neutron-neutron correlations.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Relation between single-molecule properties and phase behavior of intrinsically disordered proteins

Significance Some proteins can undergo phase separation in vivo to form membraneless organelles with distinct physiological functions. In many cases, the driving force is believed to be the intrinsically disordered domains that lack regular structural features and can phase separate in vitro. Relating protein sequences to phase-separation propensity is the goal of this work. Toward this goal, we highlight the connection between the temperature where a single chain collapses, the temperature where interactions within a dilute solution favor self-association, and the critical temperature of phase separation. Our results show that these characteristic temperatures are highly correlated, suggesting that experiments performed in dilute conditions may be used to predict phase separation.

59 BASIC BIOLOGICAL SCIENCES↗

Temperature and CO 2 interactively drive shifts in the compositional and functional structure of peatland protist communities

Microbes affect the global carbon cycle that influences climate change and are in turn influenced by environmental change. Here, we use data from a long-term whole-ecosystem warming experiment at a boreal peatland to answer how temperature and CO 2 jointly influence communities of abundant, diverse, yet poorly understood, non-fungi microbial Eukaryotes (protists). These microbes influence ecosystem function directly through photosynthesis and respiration, and indirectly, through predation on decomposers (bacteria and fungi). Using a combination of high-throughput fluid imaging and 18S amplicon sequencing, we report large climate-induced, community-wide shifts in the community functional composition of these microbes (size, shape, and metabolism) that could alter overall function in peatlands. Importantly, we demonstrate a taxonomic convergence but a functional divergence in response to warming and elevated CO 2 with most environmental responses being contingent on organismal size: warming effects on functional composition are reversed by elevated CO 2 and amplified in larger microbes but not smaller ones. Furthermore, these findings show how the interactive effects of warming and rising CO 2 levels could alter the structure and function of peatland microbial food webs—a fragile ecosystem that stores upwards of 25% of all terrestrial carbon and is increasingly threatened by human exploitation.

54 ENVIRONMENTAL SCIENCES↗

Breeding of microbiomes conferring salt tolerance to plants

Microbiome breeding through host-mediated selection is a technique to artificially select for microbiomes conferring beneficial properties to plants. Using a systematic selection protocol that maximises the heritability of microbiome effects, transmission fidelity, and microbiome stability through multiple selection cycles, we previously developed root-associated microbial communities conferring sodium and aluminium tolerance to Brachypodium distachyon, a model for cereal crops. Here, we explore the physiological mechanisms underlying our selected microbiomes’ effect on plant fitness and analyse how our selection protocol shaped the composition and structure of these microbiomes. We analysed the effects of our selected microbiomes on plant fitness and tissue-nutrient concentration, then used 16S rRNA amplicon sequencing to examine microbial community composition and co-occurrence network patterns. Our sodium-selected microbiomes reduced leaf sodium concentration by ~ 50%, whereas the aluminium-selected microbiomes had no effect on leaf-tissue nutrient concentration, suggesting different mechanisms underlying the microbiome-mediated stress tolerance. By testing the selected microbiomes in a cross-fostering experiment, we show that our artificially selected microbiomes attained (a) ecological robustness contributing to transplantability (i.e. inheritance) of microbiome-encoded effects between plants; and (b) network features identifying key bacteria promoting salt-stress tolerance. Combined, these findings elucidate critical mechanisms underlying host-mediated artificial selection as a framework to breed microbiomes with targeted benefits for plants under salt stresses, with significant implications for sustainable agriculture.

59 BASIC BIOLOGICAL SCIENCES↗

XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent

The rice receptor kinase XA21 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae ( Xoo ), the causal agent of rice bacterial blight disease. To investigate the relationship between the expression level of XA21 and resulting resistance, we generated independent HA-XA21 transgenic rice lines accumulating the XA21 immune receptor fused with an HA epitope tag. Whole-genome sequence analysis identified the T-DNA insertion sites in sixteen independent T0 events. Further, through quantification of the HA-XA21 protein and assessment of the resistance to Xoo strain PXO99 in six independent transgenic lines, we observed that XA21-mediated resistance is dose dependent. In contrast, based on the four agronomic traits quantified in these experiments, yield is unlikely to be affected by the expression level of HA-XA21 . These findings extend our knowledge of XA21-mediated defense and contribute to the growing number of well-defined genomic landing pads in the rice genome that can be targeted for gene insertion without compromising yield.

60 APPLIED LIFE SCIENCES↗

Model experiments to evaluate vortex dissipation devices proposed for installation on or near aircraft runways

The effectiveness of various vortex dissipation devices proposed for installation on or near aircraft runways is evaluated on basis of results of experiments conducted with a 0.03-scale model of a Boeing 747 transport aircraft in conjunction with a simulated runway. The test variables included type of vortex dissipation device, mode of operation of the powered devices, and altitude, lift coefficient and speed of the generating aircraft. A total of fifteen devices was investigated. The evaluation is based on time sequence photographs taken in the vertical and horizontal planes during each run.

Kohl, R. E.↗

DejaVu: A Monitoring Tool for First-Order Temporal Logic

Runtime Verification (rv) is aimed at analyzing individual execution traces and temporal behaviors observed from running programs and systems. Its traditional purpose is in detecting the lack of conformance with respect to a formal specification. While very early rv systems were based on specifications given in some form of propositional temporal logic, recent efforts have focused on monitoring so-called parametric specifications over events that carry data. Since a monitor for such specifications has to store observed data, the challenge is to have an efficient representation and manipulation of data. The fundamental problem is that the actual values of the data are not necessarily bounded or provided in advance. In this paper, we describe our monitoring tool, DejaVu, which implements our algorithm [HPU17] for monitoring first-order past linear-time temporal logic over a sequence of events that carry data. We propose the use of Binary Decision Diagrams (bdds) [Bry86] for representing and manipulating sets of observed data since (1) bdds provide highly compact representations, (2) operations over bdds, in particular complementation, are very efficient, and (3) the monitor construction for the propositional case shown in [HR02] naturally extends to bdds. Our experiments show a substantial improvement in performance compared to a related tool.

Ulus, Dogan↗

Experiment definition phase shuttle laboratory LDRL-10.6 experiment

The 10.6 microns laser data relay link (LDRL 10.6) program was directed to applying optical communications to NASA's wideband data transmission requirements through the 1980's. The LDRL consists of a transmitter on one or more low earth orbit satellites with an elliptical orbit satellite receivers. Topics discussed include: update of the LDRL design control table to detail the transmitter optical chain losses and to incorporate the change to a reflective beam pre-expander; continued examination of the link establishment sequence, including its dependence upon spacecraft stability; design of the transmitter pointing and tracking control system; and finalization of the transmitter brassboard optical and mechanical design.

Source record↗

Heat Transfer and Interfacial Flow Physics of Microgravity Flow Boiling in Single-Side-Heated Rectangular Channel with Subcooled Inlet Conditions – Experiments Onboard the International Space Station

This study is the culmination of a long-term collaborative effort between researchers from the Purdue University Boiling and Two-Phase Flow Laboratory (PU-BTPFL) and the NASA Glenn Research Center to investigate gravitational effects on flow boiling and flow condensation. The science and design concepts for this large-scale effort were initiated in 2011 and included several studies detailing various aspects of two-phase fluid physics in both Earth gravity and microgravity, culminating in construction of the large-scale experimental facility named “Flow Boiling and Condensation Experiment (FBCE)”. The experiment was launched to the International Space Station (ISS) in August 2021. Following the successful installation of FBCE, equipped with the Flow Boiling Module (FBM), onboard the ISS and completion of several safety checks, flow boiling experiments were performed for five months from February 2022 until July 2022. This resulted in a large flow boiling database covering broad ranges of operating parameters and heating configurations spanning several research objectives. This study investigates microgravity flow boiling of n-perfluorohexane with subcooled inlet in a single-side-heated rectangular channel of dimensions 114.6-mm heated length, 2.5-mm heated width, and 5.0-mm height. Key operating parameters investigated are mass velocity (199.90 – 3200.13 kg/m 2 s), inlet subcooling (0.10 – 45.76°C), and inlet pressure (113.30 – 164.29 kPa). Images and image sequences acquired via high-speed-video are presented to elucidate the interfacial flow physics. To analyze and explain the effects of various parameters in microgravity, heat transfer results are presented as flow boiling curves, streamwise profiles of wall temperature and heat transfer coefficient, and parametric trends of local and averaged heat transfer coefficient. Mass velocity and inlet subcooling significantly influenced most of the aforementioned aspects of flow boiling, whereas effects of inlet pressure were comparatively insignificant. Although the data and observed flow physics might be different, the parametric effects and trends in microgravity are similar to vertical upflow in Earth gravity. Some cases, especially low mass velocities, high heat fluxes, and large degrees of inlet subcooling, experienced temporally anomalous flow behaviors caused by two-phase flow instabilities manifesting as flow reversals and resulted in deviations in overall trends. Severe thermodynamic non-equilibrium is observed throughout the channel. Overall, FBCE’s ISS experiments were successful for subcooled inlet with single-sided heating of rectangular channel, and the collected data well established the various effects on flow boiling physics in highly controlled long-term microgravity conditions.

flow boiling↗

Contributions of Planetary Science to Studies of Early Biosphere Evolution

The history of impact cratering on the Moon, and extrapolations of crater chronologies to the inner planets, suggests that the late accretionary history of the Earth overlapped with other crucial events in the its history, including the origin of terrestrial life. This evidence, acquired from studies of other planetary bodies in the inner solar system, has profoundly affected how we view the early history of the Earth and evolution of the biosphere. Pre-biotic chemical evolution and the origin of life would have been delayed by the probable existence of a global magma ocean until -4.2 Ga. The early crust was largely destroyed by recycling, thus accounting for the sparse Archean record on Earth. Once life had developed, large impacts may have extinguished it several times before it finally gained a foothold. Potentially sterilizing impacts could have occurred as late as 3.7 Ga. At the very least, large impacts would have forced the biosphere through major environmental "bottlenecks" thereby canalizing its subsequent evolution. One legacy of these early events may be the structure of the present RNA-tree which indicates that extreme thermophiles are primitive within the Archaea, and may be the last common ancestors of life. By 3.5 Ga, marine sedimentary sequences contain unequivocal microbial fossils that attest to the presence of a terrestrial biosphere. The diversity of microbial forms present in these earliest fossil assemblages implies a preceding interval of evolution during which major evolutionary advances (e.g. photosynthesis) could have taken place. Evidence cited above places the origin of life within the interval 3.5 and 4.2 Ga, a period of 700 Ma. Thus, it appears that terrestrial life not only evolved rapidly, but perhaps more than once. This expands the possibilities that life may have also developed elsewhere. Of the other planets in our solar system, Mars holds the greatest chance of having developed life. But, the present surface of Mars is hostile to life. Liquid water, regarded as essential for living systems, is unstable on the surface of Mars due to the low atmospheric pressure. The results of the Viking Lander biology experiments established that organic molecules are not present in the regolith of Mars, forcing the exobiological community to consider new ways of exploring for Martian life. Older, heavily cratered terranes on Mars show geomorphic evidence for abundant water between 3.0- 4.0 Ga. It is quite possible that life developed on Mars during this time, as it did on the Earth. The present focus for Mars Exobiology lies in the search for a fossil record. Archean-aged crust, while mostly missing on Earth, appears to be widespread in ancient cratered highlands of Mars, and aqueous mineral deposits within such sequences may hold crucial fossil evidence for an early Martian biosphere.

Farmer, Jack D.↗

Operations and Autonomy of the Mars Pathfinder Microrover

The Microrover Flight Experiment (MFEX) is a NSAS OACT (Office of Advanced Concepts and Technology) flight experiment which, integrated with the Mars Pathfinder (MPF) lander and spacecraft system, landed on Mars on July 4, 1997.

navigation surface operations scenario commmand se↗

Experiment T001: Entry communication on the Gemini 3 mission

Water addition to the Gemini 3 exhaust plasma was studied to determine its effectiveness in the establishment of communication links during the entry portion of the flight. Attenuation levels were measured with and without water injection at uhf frequencies of 230.4 and 296.8 megahertz and at the C-band frequency of 5690 megahertz. Ultrahigh frequency signals that had been blacked out were restored to significant levels, during early portions of the water-injection sequence, by the high flow rate injection. The C-band signal was enhanced by medium and high flow rate injections during the latter portion of the injection period. The uhf signal recovered during water injection resulted in an antenna pattern that was beamed in the radial direction of injection from the spacecraft. Postflight analysis showed that the uhf recovery data were consistent with injection-penetration theory.

Schroeder, L. C.↗

PERCEPTIVE: an R shiny $\underline{p}$ipelin$\underline{e}$ for the p$\underline{r}$edi$\underline{c}$tion of $\underline{ep}$igenetic modula$\underline{t}$ors $\underline{i}$n no$\underline{v}$el sp$\underline{e}$cies

Epigenetic processes are central to regulating gene expression, genome stability, and metabolic function across the tree of life; yet, their roles remain underexplored in microalgae, especially as new species continue to be identified and characterized. This is likely due to the cumbersome nature and species-dependent attributes of epigenetic wet-lab methodologies, which preclude the rapid identification of epigenetic modifications and modulators. However, there is high conservation of epigenetic processes from budding yeast to humans; in many cases, one may infer how behavior and function are epigenetically regulated in novel species by identifying epigenetic modulators, or the proteins responsible for conferring epigenetic modifications. Here, to this end, we have developed a graphical software package, titled PERCEPTIVE (pipeline for the prediction of epigenetic modulators in novel species). This platform solely uses the genomic sequence of an algal species, and preexisting information from other model organisms, to predict the epigenetic modulators and associated modifications in algae. Predictions are presented to the user in a graphical interface, which provides literature-based interpretation of results, enabling users to quickly understand potential epigenetic processes in their algal species of interest and plan follow-up experiments. To test PERCEPTIVE, we predicted epigenetic modulators in several feedstock candidate algae species. To validate these predictions, wet-lab studies were performed, including mass spectrometry; these results underscore the high accuracy of PERCEPTIVE predictions. Overall, PERCEPTIVE represents a powerful in silico tool for the research and manipulation of algal species, which does not require a priori knowledge of epigenetics and is accessible to a broad set of investigators.

59 BASIC BIOLOGICAL SCIENCES↗