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Building a virtual planet

The virtual Planetary Laboratory (VPL) is a recently funded 5-yr project, which seeks toimprove our understanding of the range of plausible environments and the likely signatures for life on extrasolar terrestrial planets. To achieve these goals we are developing a suite of innovative modeling tools to simulate the environments and spectra of extrasolar planets. The core of the VPL IS a coupled radiative transfer/climate/chemistry model, which is augmented by interchangeable modules which characterize geological, exogenic, atmospheric escape, and life processes. The VPL is validated using data derived from terrestrial planets within our own solar system. The VPL will be used to explore the plausible range of atmospheric composittions and globally averaged spectra for extrasolar planets and for early Earth, and will improve our understanding of the effect of life on a planet's atmospheric spectrum and composition. The models will also be used to create a comprehensive spectral catalog to provide recommendations on the optimum wavelength range, spectral resolution, and instrument sensitivity required to characterize extrasolar terrestrial planets. Although developed by our team, the VPL is envisioned to be a comprehensive and flexible tool, which can be collaboratively used by the broader planetary science and astrobiology communities. This presentation will describe the project concept, the tasks involved, and will outline current progress to date. This work is funded by the NASA Astrobiology Institute.

astrobiology↗

Autonomy Software Architecture for LORAX (Life On ice Robotic Antarctic eXplorer)

LORAX is a robotic astrobiological study of the ice field surrounding the Carapace Nunatak near the Allan Hills in Antarctica. The study culminates in a l00km traverse, sampling the ice at various depths (from surface to 10cm) at over 100 sites to survey microbial ecology and to record environmental parameters. The autonomy requirements from LORAX are shared by many robotic exploration tasks. Consequently, the LORAX autonomy architecture is a general architecture for on-board planning and execution in environments where science return is to be maximized against resource limitations and other constraints.

Jonsson, Ari↗

Astrobiology

Astrobiology is the study of the origins, evolution and distribution of life in the universe. It provides a biological perspective to many areas of NASA research, linking such endeavors as the search for habitable planets beyond our solar system, exploration missions to Mars and Europa, and efforts to understand the origin and early evolution of life. Astrobiology addresses three fundamental questions: How does life begin and develop? Does life exist elsewhere in the universe? What is the future of life on Earth and beyond? This talk will address our concepts about the definition of life, how life might have begun, and how our blaspheme and planet have co-evolved for billions of years. The talk will explore how the perspectives gained from interdisciplinary research in the biological, geological and space sciences will prepare us to search for habitable environments and biospheres elsewhere in the Universe.

DesMarais, David↗

Astrobiology

Astrobiology is the study of the origins, evolution and distribution of life in the universe. It provides a biological perspective to many areas of NASA research, linking such endeavors as the search for habitable planets beyond our solar system, exploration missions to Mars and Europa, and efforts to understand the origin and early evolution of life. Astrobiology addresses three fundamental questions: How does life begin and develop? Does life exist elsewhere in the universe What is the future of life on Earth and beyond? This talk will address our concepts about the definition of life, how life might have begun, and how our blaspheme and planet have co-evolved for billions of years. The talk will explore how the perspectives gained from interdisciplinary research in the biological, geological and space sciences will prepare us to search for habitable environments and blasphemes elsewhere in the Universe.

DesMarais, David↗

Technical Challenges of Drilling on Mars

In the last year, NASA's Mars science advisory committee (MEPAG: Mars Exploration Payload Advisory Group) has formally recommended that deep drilling be undertaken as a priority investigation to meet astrobiology and geology goals. This proposed new dimension in Mars exploration has come about for several reasons. Firstly, geophysical models of the martian subsurface environment indicate that we may well find liquid water (in the form of brines) under ground-ice at depths of several kilometers near the equator. On Earth we invariably find life forms associated with any environmental niche that supports liquid water. New data from the Mars Global Surveyor have shown that the most recent volcanism on Mars is very young so we cannot rule out contemporary volcanism -- in which case subsurface temperatures consistent with having water in its liquid phase may be found at relatively shallow depths. Secondly, in recent decades we have learned to our surprise that the Earth's subsurface (microbial) biosphere extends to depths of many kilometers and this discovery provides the basis for planning to explore the martian subsurface in search of ancient or even extant microbial life forms. We know (from Viking measurements) that all the biogenic elements (C, H, O, N, P, S) are available on Mars. What we therefore hope to learn is whether or not the evolution of life is inevitable given the necessary ingredients and, by implication, whether the Universe may be teeming with life. The feasibility of drilling deep into the surface of Mars has been the subject of increasing attention within NASA (and more recently among some of its international partners) for several years and this led to a broad-based feasibility study carried out by the Los Alamos National Laboratory and, subsequently, to the development of several hardware prototypes. This paper is intended to provide a general survey of that activity.

Briggs, Geoffrey↗

Biomolecular Analysis Capability for Cellular and Omics Research on the International Space Station

International Space Station (ISS) assembly complete ushered a new era focused on utilization of this state-of-the-art orbiting laboratory to advance science and technology research in a wide array of disciplines, with benefits to Earth and space exploration. ISS enabling capability for research in cellular and molecular biology includes equipment for in situ, on-orbit analysis of biomolecules. Applications of this growing capability range from biomedicine and biotechnology to the emerging field of Omics. For example, Biomolecule Sequencer is a space-based miniature DNA sequencer that provides nucleotide sequence data for entire samples, which may be used for purposes such as microorganism identification and astrobiology. It complements the use of WetLab-2 SmartCycler"TradeMark", which extracts RNA and provides real-time quantitative gene expression data analysis from biospecimens sampled or cultured onboard the ISS, for downlink to ground investigators, with applications ranging from clinical tissue evaluation to multigenerational assessment of organismal alterations. And the Genes in Space-1 investigation, aimed at examining epigenetic changes, employs polymerase chain reaction to detect immune system alterations. In addition, an increasing assortment of tools to visualize the subcellular distribution of tagged macromolecules is becoming available onboard the ISS. For instance, the NASA LMM (Light Microscopy Module) is a flexible light microscopy imaging facility that enables imaging of physical and biological microscopic phenomena in microgravity. Another light microscopy system modified for use in space to image life sciences payloads is initially used by the Heart Cells investigation ("Effects of Microgravity on Stem Cell-Derived Cardiomyocytes for Human Cardiovascular Disease Modeling and Drug Discovery"). Also, the JAXA Microscope system can perform remotely controllable light, phase-contrast, and fluorescent observations. And upcoming confocal microscopy capability will allow for optical sectioning of biological tissues to determine microanatomical localization of biomarkers. Furthermore, NASA's geneLAB effort addresses integration of genomic, epigenomic, transcriptomic, proteomic and metabolomic datasets, by applying an innovative open source science platform for multi-investigator high throughput utilization of the ISS. In sum, the expanding ISS capability for analysis of biomolecules is enabling innovative research in a broad spectrum of areas such as cellular and molecular biology, biotechnology, tissue engineering, biomedicine, and Omics, providing manifold benefits for humanity.

Guinart-Ramirez, Y.↗

Detecting Organic Compounds Released from Iron Oxidizing Bacteria using Sample Analysis at Mars (SAM) Like Instrument Protocols

Mars is a planet of great interest for Astrobiology since its past environmental conditions are thought to have been favourable for the emergence life. At present, the Red Planet is extremely cold and dry and the surface is exposed to intense UV and ionizing radiation, conditions generally considered to be incompatible with life as we know it on Earth. It was proposed that the shallow subsurface of Mars, where temperatures can be above freezing and liquid water can exist on rock surfaces, could harbor chemolithoautotrophic bacteria such as the iron oxidizing microorganism Pseudomonas sp. HerB. The Mars Science Laboratory (MSL) mission will provide the next opportunity to carry out in situ measurements for organic compounds of possible biological origin on Mars. One instrument onboard MSL, called the Sample Analysis at Mars (SAM) instrument suite, will carry out a broad and sensitive search for organic compounds in surface samples using either high temperature pyrolysis or chemical extraction followed by gas chromatography mass spectrometry. We present gas chromatograph mass spectrometer (GC/MS) data on crushed olivine rock powders that have been inoculated with Pseudomonas sp. HerB at different concentrations ranging from approx 10(exp 2) to 10(exp 7) cells per gram. The inoculated olivine samples were heated under helium carrier gas flow at 500 C and the pyrolysis products concentrated using a SAM-like hydrocarbon trap set at -20 C followed by trap heating and analysis by GC/Ms. In addition, the samples were also extracted using a low temperature "one-pot" chemical extraction technique using N-methyl, N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) as the silylating agent prior to GC/MS analysis. We identified several aldehydes, thiols, and alkene nitriles after pyrolysis GC/MS analysis of the bacteria that were not found in the olivine control samples that had not been inoculated with bacteria. The distribution of pyrolysis products extracted from the bacteria was clearly distinct from similar GC/MS analyses of the carbonaceous meteorite Murchison that was dominated by sulfur containing aromatic compounds. A similar comparison, if organic compounds are detected by SAM on Mars, could be useful to help discriminate between meteoritic or biological origins.

Glavin, D. P.↗

Titan In-situ Resource Utilization (ISRU) Sample Return (TISR)

Titan is unique in the outer solar system in that it is the only moon with a thick atmosphere, and the only body in the solar system outside the Earth with liquid seas on its surface. The Titanian oceans, however, are seas of liquid hydrocarbons, and the rocks on the surface are solid water ice. Like other icy Moons of the outer solar system, beneath the ice crust, Titan also has a subsurface ocean. Rodriguez et al. refer to it as the “world with two oceans”, an organic-rich body with interior-surface-atmosphere interactions that are comparable in complexity to the Earth. [1] Titan is scientifically fascinating in many ways [2], [3], [4]. The Compass Team will emphasize just one here: Titan is a high priority target for astrobiology [4] [5] [6] [7] [8] [9] [10] [11]. It is a world with a surface and atmosphere rich in the complex organic compounds known as tholins. A detailed understanding of the nature of these complex compounds will require an analysis using a full laboratory on Earth. Because of its value to understanding the organic compounds of the outer solar system which may be the primordial building-blocks of life, return of samples from Titan to laboratories on Earth will be the primary goal of this mission. While this would give unprecedented science return, returning even a small sample from Titan using conventional technology would be tremendously difficult. Saturn is almost a billion miles from the Earth, about thirteen times farther than Mars. A return mission to Saturn requires such a large total-mission ∆V that, with conventional technology, the mass ratios required are prohibitive. Such a sample return would truly be “mission incredible.” But to date, a sample return mission from so distant a target has been assumed to be, not merely incredible, but mission impossible. The Compass Team has proposed [2] [12] that by manufacturing the propellant for the return to Earth using the resources available on Titan, such a mission becomes possible. The task of this report is to show that it is reasonable with credible space technology.

Titan↗

Habitability Models for Astrobiology

Habitability has been generally defined as the capability of an environment to support life. Ecologists have beenusing Habitat Suitability Models (HSMs) for more than four decades to study the habitability of Earth fromlocal to global scales. Astrobiologists have been proposing different habitability models for some time, with lit-tle integration and consistency among them, being different in function to those used by ecologists. Habitabilitymodels are not only used to determine whether environments are habitable, but they also are used to charac-terize what key factors are responsible for the gradual transition from low to high habitability states. Here wereview and compare some of the different models used by ecologists and astrobiologists and suggest how theycould be integrated into new habitability standards. Such standards will help improve the comparison and charac-terization of potentially habitable environments, prioritize target selections, and study correlations between habit-ability and biosignatures. Habitability models are the foundation of planetary habitability science, and the synergybetween ecologists and astrobiologists is necessary to expand our understanding of the habitability of Earth,the Solar System, and extrasolar planets.

Habitability↗

The NASA Astrobiology Program’s Professional Advancement Workshop Series: Next Steps

Introduction: In the modern-day competitive job search, it is not enough for a candidate to have an excellent academic resume. Candidates must also present themselves well in various styles of interviews, have managerial skills, and be capable of engaging in complex interpersonal relationships and team building. The NASA Astrobiology Program’s Professional Advancement Workshop Series (PAWS) addresses the need for professional skills development that is not traditionally taught in graduate programs. PAWS is designed to supplement academic teachings by providing a space where early career scientists can learn new skills to help them explore, interview for, and be hired in the jobs and careers they want. This is especially true for jobs that are outside of the traditional academic “pipeline” (the path of student to postdoc to tenure-track professor). PAWS embraces the braided river model of career development [1], which emphasizes flexibility as a scientist’s needs change throughout their life. PAWS also presents an opportunity for other early career scientists to meet and network with each other outside of conferences and other formal events. This is especially important as the world emerges from the ongoing COVID-19 pandemic. Finally, PAWS creates an informative space full of resources available to everyone, which lowers institutional barriers regarding knowledge of opportunities and potentially provides mentorship to those in need. Both items are identified as supportive of a more diverse and inclusive future workforce [2–4]. PAWS began in August 2021 and was originally intended to last until the Astrobiology Science Conference in May 2022. The NASA Astrobiology Program initially sponsored PAWS, allowing the PAWS Leadership to bring in expert speakers from outside of NASA. These external speakers exposed the event attendees to new techniques and perspectives. PAWS has been able to continue beyond May 2022 because of many people volunteering their time for panels and a good balance between panels and expert-led workshops. Here, we share how PAWS works, what the results have been so far, and the future plans for PAWS.

Worshop↗

The Advanced Technology Large Aperture Space Telescope (ATLAST): Science Drivers and Technology Developments

The Advanced Technology Large-Aperture Space Telescope (ATLAST) is a concept for an 8-meter to 16-meter UVOIR space observatory for launch in the 2025-2030 era. ATLAST will allow astronomers to answer fundamental questions at the forefront of modern astrophysics, including "Is there life elsewhere in the Galaxy?" We present a range of science drivers and the resulting performance requirements for ATLAST (8 to 16 milliarcsecond angular resolution, diffraction limited imaging at 0.5 m wavelength, minimum collecting area of 45 square meters, high sensitivity to light wavelengths from 0.1 m to 2.4 m, high stability in wavefront sensing and control). We also discuss the priorities for technology development needed to enable the construction of ATLAST for a cost that is comparable to current generation observatory-class space missions. Keywords: Advanced Technology Large-Aperture Space Telescope (ATLAST); ultraviolet/optical space telescopes; astrophysics; astrobiology; technology development.

Postman, Marc↗

Atacama Rover Astrobiology Drilling Studies Project: Final Year

The Atacama Rover Astrobiology Drilling Studies (ARADS) project, a simulated Mars rover biomarker detection mission, was iteratively developed over four years from 2015 -2019, including three NASA centers, the Centro de Astrobiologia, Johns Hopkins University, Honeybee Robotics, Maxar and the University of Antofagasta. The final (4th) ARADS field season, in 2019, tested an integrated mobile life-prospecting platform loosely inspired by the 2000’s Astrobiology Field Laboratory concept, with a 1m rotary-percussive drill and sample transfer robot arm that fed three astrobiology instruments operating in-situ on the KREX2 medium rover prototype. A fourth instrument was field tested earlier in 2019 due to flight mission requirements. In the final ARADS field deployment in September 2019, the project conducted a remote mission operation simulation (Stoker 2022)demonstrating sample drilling, acquisition and transfer into the rover instruments (while minimizing cross-contamination), performing in-situ analysis of the samples, and returning the results to a remote science operations team(which commanded the daily science goals and uploaded operations sequences).

astrobiology↗

Chapter 2: What Is Life?

The question “What is life?” has existed since the beginning of recorded history. However, the scientific and philosophical contexts of this question have changed and been refined as advancements in technology have revealed both fine details and broad connections in the network of life on Earth. Understanding the framework of the question “What is life?” is central to formulating other questions such as “Where else could life be?” and “How do we search for life elsewhere?” While many of these questions are addressed throughout the Astrobiology Primer 3.0, here this chapter gives historical context for defining life, highlights conceptual characteristics shared by all life on Earth as well as key features used to describe it, discusses why it matters for astrobiology, and explores both challenges and opportunities for finding an informative operational definition.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Astrobiology: Study of the Living Universe

Astrobiology is defined as the study of the living universe. This endeavor encompasses the use of space to understand life's origin, evolution, and destiny in the universe. Life's origin refers to understanding the origin of life in the context of the origin and diversity of planetary systems. Life's evolution refers to understanding how living systems have adapted to Earth's changing environment, to the all-pervasive force of gravity, and how they may adapt to environments beyond Earth. Life's destiny refers to making long-term human presence in space a reality, and laying the foundation for understanding and managing changes in Earth's environment. This lecture will explore the development of this field of inquiry, the science questions to be examined, and the mechanisms available for participation by the scientific community.

DeVincenzi, Donald L.↗

Ballooning for Biologists: Mission Essentials for Flying Life Science Experiments to Near Space

Despite centuries of scientific balloon flights, only a handful of experiments have produced biologically-relevant results. Yet unlike orbital spaceflight, it is much faster and cheaper to conduct biology research with balloons, sending specimens to the near space environment of Earth’s stratosphere. Samples can be loaded the morning of a launch and sometimes returned to the laboratory within one day. The National Aeronautics and Space Administration (NASA) flies large, unmanned scientific balloons from all over globe, with missions ranging from hours to weeks in duration. A payload in the middle portion of the stratosphere (~35 km above sea level) will be exposed to an environment similar to the surface of Mars: temperatures as cold as -100 °C, atmospheric pressure at a thin 0.9 kPa, relative humidity levels < 1%, and a harsh illumination of ultraviolet (UV) and cosmic radiation levels (about 100 W/m 2 and 0.07 mGy/d, respectively) that can be obtained nowhere else on the surface of the Earth, including environmental chambers and particle accelerator facilities attempting to simulate space radiation effects. Considering operational advantages of ballooning and the fidelity of space-like stressors in the stratosphere, researchers in aerobiology, astrobiology, and space biology can benefit from balloon flight experiments as an intermediary step on the extraterrestrial continuum (ground, low Earth orbit, and deep space studies). Our review targets biologists with no background or experience in scientific ballooning. We will provide an overview of balloon operations, topics that can be uniquely addressed in the stratosphere, and a roadmap for developing payloads to fly with NASA.

NASA Balloon Program↗

Development of a NASA 2018 Mars Landed Mission Concept

Fundamental to NASA's Mars Exploration Program (MEP) is an ongoing development of an integrated and coordinated set of possible future candidate missions that meet fundamental science and programmatic objectives of NASA and the Mars scientific community. In the current planning horizon of the NASA MEP, a landed mobile surface exploration mission launching in the 2018 Mars launch opportunity exists as a candidate project to meet MEP in situ science and exploration objectives. This paper describes the proposed mission science objectives and the mission implementation concept developed for the 2018 opportunity. As currently envisioned, this mission concept seeks to explore a yet-to-be-selected site with high preservation potential for physical and chemical biosignatures, evaluate paleoenvironmental conditions, characterize the potential for preservation of biosignatures, and access multiple sequences of geological units in a search for evidence of past life and/or prebiotic chemistry at a site on Mars.

Mars Astrobiology Explorer- Cacher (MAX-C)↗

The CAESAR New Frontiers Comet Sample Return Mission

The Comet Astrobiology Exploration Sample Return (CAESAR) mission is one of two finalists selected by NASA for Phase A study in the New Frontiers program. CAESAR will acquire a minimum of 80 grams of material from the surface of comet 67P/Churuyumov-Gerasimenko and return it to Earth for laboratory analysis. CAESAR preserves much of the science of a cryogenic sample return by retaining volatiles in a dedicated reservoir securely separated from the solid sample. Comet 67P was selected based on its favorable orbital geometry and the risk reduction and scientific context provided by the ESA (European Space Agency)'s Rosetta mission. CAESAR's objectives are to understand the origins of the Solar System starting materials and how these components came together to form planets and give rise to life. We also seek to resolve the conflicting views of comet origins arising from the Stardust and Rosetta missions. While the greater than 1 micron solids returned by Stardust originated in the hot, inner solar nebula, measurements by Rosetta suggest 67P volatiles formed at cryogenic temperatures and remained unchanged for billions of years. This dichotomy provides the rationale for returning both solid and gaseous samples.

CAESAR New Frontiers↗

Drilling Automation Demonstrations in Subsurface Exploration for Astrobiology

This project proposes to study subsurface permafrost microbial habitats at a relevant Arctic Mars-analog site (Haughton Crater, Devon Island, Canada) while developing and maturing the subsurface drilling and drilling automation technologies that will be required by post-2010 missions. It builds on earlier drilling technology projects to add permafrost and ice-drilling capabilities to 5m with a lightweight drill that will be automatically monitored and controlled in-situ. Frozen cores obtained with this drill under sterilized protocols will be used in testing three hypotheses pertaining to near-surface physical geology and ground H2O ice distribution, viewed as a habitat for microbial life in subsurface ice and ice-consolidated sediments. Automation technologies employed will demonstrate hands-off diagnostics and drill control, using novel vibrational dynamical analysis methods and model-based reasoning to monitor and identify drilling fault states before and during faults. Three field deployments, to a Mars-analog site with frozen impact crater fallback breccia, will support science goals, provide a rigorous test of drilling automation and lightweight permafrost drilling, and leverage past experience with the field site s particular logistics.

Glass, Brian↗