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Synthetic Biology to Support Human Exploration of Deep Space

The International Space Station (ISS) has enabled a continuous human presence in space since November 2000. The ISS is in low Earth orbit, facilitating regular resupply missions to deliver air, water, food, spare parts, and science experiments. NASA’s Moon to Mars campaign seeks to return humans to the Moon and prepare for crewed missions to Mars. Increased distance from Earth poses logistical challenges to provide all resources needed by humans for deep space missions. NASA Ames Research Center is conducting a series of synthetic biology projects to test the use of microbes for on-demand biosynthesis of human micronutrients. The BioNutrients spaceflight experiments test an implementation concept to produce fermented food products in which microbe growth enhances micronutrient content. On-demand production of carotenoids was engineered into two yeast (Saccharomyces cerevisiae) strains that have been tested in nearly 5 years of storage. One of the BioNutrients strains will be incorporated into the Lunar Explorer Instrument for Space Biology Applications (LEIA) project. LEIA is developing an instrument suite to be delivered to the lunar south pole region by the Commercial Lunar Payload Services (CLPS) program. The LEIA instrument suite will be used to measure multiple yeast strains for growth, metabolic activity, and synthetic biology-enabled production of carotenoids, while taking real time measurements of biologically relevant radiation exposure on the lunar surface. LEIA data will be used to assess the impact of lunar surface radiation and reduced gravity on the production of engineered traits.

Synthetic Biology↗

The NASA Specialized Center of Research and Training (NSCORT) in Gravitational Biology

The Life Sciences Division of NASA has initiated a NASA Specialized Centers of Research and Training (NSCORT) program. Three Centers were designated in late 1990, as the culmination of an in-depth peer review analysis of proposals from universities across the nation and around the world. Kansas State University was selected as the NSCORT in Gravitational Biology. This Center is headquartered in the KSU Division of Biology and has a research, training, and outreach function that focuses on cellular and developmental biology.

NASA Discipline Cell Biology↗

Space Biology Beyond LEO Instrumentation & Science Series Science Working Group 2022 Annual Report

Humans are poised to explore deep space: the realm of space beyond Earth's orbit. NASA will soon send humans back to the Moon with the Artemis program, and is developing programs to support crewed missions to Mars. Human exploration of such new environments demands fundamental research that can provide the knowledge necessary to ensure the safety of explorers and aid in the development of a sustainable presence in space. Accordingly, the Agency's Moon to Mars objectives1 include three goals in the area of Human Biological Sciences (HBS-1, -2, -3), with the aim to "Advance understanding of how biology responds to the environments of the Moon, Mars, and deep space to advance fundamental knowledge, support safe, productive human space missions and reduce risks for future exploration." Advancing this understanding is a task that is both complex-- comprising diverse organisms, processes, and methods-- and difficult-- because the very aspects of deep space that we strive to understand are the aspects that make it hard to conduct research in that environment. This report of the Beyond LEO Instrumentation & Science Series Science Working Group (BLISS-SWG) represents input from a group of scientists from diverse disciplines within the space biology research and engineering community on the nature of the science and technology that can be used to achieve those aims.

Space Biology↗

Aerospace medicine and biology. an annotated bibliography, 1954 literature, volume iii

While this third volume of the bibliography was in preparation, a number of changes and new developments took place which, it is hoped, will be instrumental in furthering the bibliography's main objective: to provide as rapidly as possible comprehensive coverage of the aerospace-medical and bio-astronautical literature. To begin with, it will be noted that the title of the compilation has been changed from Aviation Medicine to Aerospace Medicine and Biology. This will bring into relief the fact that the general subject scope of the bibliography has been expanded to include new areas of interest in space-medical and space-biological research. Thus, to the subject fields heretofore covered by the bibliography (a full listing of which may be found in the Preface to Volume 2) new ones have been added, such as Biological Rhythms, Biological Orientation, Closed Ecological Systems, Physiological and Psychological Effects of Heat, Cold, Isolation, Restraint, and many others. Another important change was administrative in nature. Sponsorship of the bibliography for the fiscal year 1959/60 was taken over by the U. S. Advanced Research Project Agency (ARPA), the U. S. Aeronautics and Space Administration (NASA), and the Defense Research Board of Canada. Under a substantially increased budget, the Library of Congress was enabled to produce two volumes during the fiscal year. The Library also agreed to prepare selected abstracts from the current aerospace and bio-astronautic literature, which are published monthly in Aerospace Medicine, the official organ of the Aerospace Medical Association.*) If the present rate of production can be maintained, it will be possible to bring the bibliography up to date within about three years. In format, style, and arrangement, this volume follows closely the preceding ones. References are arranged alphabetically by author; abstracts are informative; and full bibliographic information is provided, including foreign and translated titles, volume and issue numbers, place of publication (if foreign), inclusive paging, full dates, and library call numbers or other location symbols. While the bulk of the publication is devoted to journal articles, reports, and monographs published during the year 1954, items published in 1952 or 1953 not included in the preceding volumes have been incorporated in this volume. For quick identification, the publication years of these off-year references have been printed next to the first author's name. As before, cumulated corporate and secondary author and subject indexes are included, the latter being revised and enlarged considerably, in keeping with the over-all expansion of subject scope.

Aerospace medicine↗

Biological cycling of atmospheric trace gases

A detailed critical review was conducted of present knowledge of the influence of biological processes on the cycling of selected atmospheric gas constituents--methane, carbon monoxide, and gaseous compounds of nitrogen (nitrous oxide, ammonia, nitric oxide, and nitrogen dioxide) and sulfur (hydrogen sulfide and sulfur dioxide). The identification was included of biological and other sources of each gas, a survey of abundance measurements reported in the literature, and a review of the atmospheric fate of each contituent. Information is provided on which to base conclusions regarding the importance of biological processes on the atmospheric distribution and surface-atmosphere exchange of each constituent, and a basis for estimating the adequacy of present knowledge of these factors. A preliminary analysis was conducted of the feasibility of monitoring the biologically influenced temporal and spatial variations in abundance of these gases in the atmosphere from satellites.

Hitchcock, D. R.↗

Biological instrumentation for the Viking 1975 mission to Mars.

A brief introduction is given on why Mars is of interest from a biological point of view, along with an overview of the Viking 1975 mission. Details are given about the four biology instruments aboard the spacecraft and the experiments for which they are to be used. These are: the carbon assimilation experiment to determine whether the soil is biologically active; the label release experiment to detect metabolic activity by the release of radioactive CO2 from C-14 labelled simple organic substrates; the gas exchange experiment to detect biological activity by repeated gas chromatography analysis of soil samples; and the light scattering experiment, where increase of scattering and decrease of light transmission would indicate the growth of organisms. Examples are given of data obtained with terrestrial soils in these experiments.

Klein, H. P.↗

Aerospace medicine and biology: A continuing bibliography with indexes, supplement 107, October 1972

This Supplement of Aerospace Medicine and Biology lists 353 reports, articles, and other documents announced during September 1972 in Scientific and Technical Aerospace Reports or in International Aerospace Abstracts. In its subject coverage, Aerospace Medicine and Biology concentrates on the biological, physiological, psychological, and environmental effects to which man is subjected during and following simulated or actual flight in the earth's atmosphere or in interplanetary space. References describing similar effects of biological organisms of lower order are also included. Such related topics as sanitary problems, pharmacology, toxicology, safety and survival, life support systems, exobiology, and personnel factors receive appropriate attention. In general, emphasis is placed on applied research, but references to fundamental studies and theoretical principles related to experimental development also qualify for inclusion.

Source record↗

Aerospace Medicine and Biology: A continuing bibliography with indexes, supplement 143

This supplement to Aerospace Medicine and Biology (NASA SP-7011) lists 251 reports, articles and other documents announced during June 1975 in Scientific and Technical Aerospace Reports (STAR) or in International Aerospace Abstracts (IAA). The first issue of the bibliography was published in July 1964; since that time, monthly supplements have been issued. In its subject coverage, Aerospace Medicine and Biology concentrates on the biological, physiological, and environmental effects to which man is subjected during and following simulated or actual flight in the earth's atmosphere or in interplanetary space. References describing similar effects of biological organisms of lower order are also included. Such related topics as sanitary problems, pharmacology, toxicology, safety and survival, life support systems, exobiology, and personnel factors receive appropriate attention. In general, emphasis is placed on applied research, but references to fundamental studies and theoretical principles related to experimental development also qualify for inclusion.

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Environmental parameters of the Tennessee River in Alabama. 2: Physical, chemical, and biological parameters

Physical, chemical and biological water quality data from five sites in the Tennessee River, two in Guntersville Reservoir and three in Wheeler Reservoir were correlated with climatological data for three annual cycles. Two of the annual cycles are for the years prior to the Browns Ferry Nuclear Power Plant operations and one is for the first 14 months of Plant operations. A comparison of the results of the annual cycles indicates that two distinct physical conditions in the reservoirs occur, one during the warm months when the reservoirs are at capacity and one during the colder winter months when the reservoirs have been drawn-down for water storage during the rainy months and for weed control. The wide variations of physical and chemical parameters to which the biological organisms are subjected on an annual basis control the biological organisms and their population levels. A comparison of the parameters of the site below the Power plant indicates that the heated effluent from the plant operating with two of the three reactors has not had any effect on the organisms at this site. Recommendations given include the development of prediction mathematical models (statistical analysis) for the physical and chemical parameters under specific climatological conditions which affect biological organisms. Tabulated data of chemical analysis of water and organism populations studied is given.

Rosing, L. M.↗

Scientific experiments in the flight of the 1977 biological satellite (draft plan)

The physiological, biological, radiobiological and radiophysical experiments planned for the 1977 biological satellite are described. The biological experiments will involve rats, higher and lower plants, insects and other biological specimens carried on the biosatellite. The responses of these organisms to weightlessness, artificial gravity, cosmic radiation particles and general flight factors will be studied. The radiophysical experiments will investigate certain properties of cosmic radiation as well as the possibility of creating electrostatic and dielectric radiation shields under actual space-flight conditions.

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Aerospace Medicine and Biology: A continuing bibliography with indexes, supplement 187

This supplement to Aerospace Medicine and Biology lists 247 reports, articles and other documents announced during November 1978 in Scientific and Technical Aerospace Reports (STAR) or in International Aerospace Abstracts (IAA). In its subject coverage, Aerospace Medicine and Biology concentrates on the biological, physiological, psychological, and environmental effects to which man is subjected during and following simulated or actual flight in the earth's atmosphere or in interplanetary space. References describing similar effects of biological organisms of lower order are also included. Emphasis is placed on applied research, but reference to fundamental studies and theoretical principles related to experimental development also qualify for inclusion. Each entry in the bibliography consists of a bibliographic citation accompanied in most cases by an abstract.

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The joint US-USSR biological satellite program

The joint US-USSR biological satellite missions carried out in 1975 and 1977 using Cosmos 782 and Cosmos 936 spacecraft, respectively, is reviewed. The experimental equipment and the biological specimens aboard the aircraft are considered, and it is noted that Cosmos 782, unlike Cosmos 936, carried no centrifuges for rats, although it did contain a centrifuge where a variety of biological specimens, including carrot tissue and fruit flies, were subjected to artificial gravity during space flight. The ground control groups, designed for biological experiments under simulated space-conditions, are taken into account. The U.S. experiments aboard the aircraft are described, with attention given to the experiments with rats, fish embryos, plants, and insects. Results of the experiments are noted, including the finding that space flight factors, especially weightlessness, have a measurable effect on the erythropoietic and musculoskeletal systems of rats

Souza, K. A.↗

Biologically mediated isotope fractionations - Biochemistry, geochemical significance and preservation in the earth's oldest sediments

Preferential metabolization of isotopically light carbon and sulfur has resulted in a fractionation of the stable isotopes of these elements on a global scale, with the light species (C-12, S-32) markedly concentrated in biogenic materials. Since the biological effects are basically retained when carbon and sulfur are incorporated in sediments, the respective fractionations are propagated into the rock section of the geochemical cycle, this having consequently caused a characteristic bipartition of both elements between 'light' and 'heavy' crustal reservoirs. Preservation of the biological isotope effects in sedimentary rocks makes it possible to trace the underlying biochemical processes back over most of the geological record. According to the available evidence, biological (autotrophic) carbon fixation arose prior to 3.5(if not 3.8) billion years ago, while the emergence of dissimilatory sulfate reduction antedates the appearance of the oldest presumably bacteriogenic sulfur isotope patterns in rocks between 2.7 and 2.8 billion years old. Hence, biological control of the terrestrial carbon and sulfur cycles has been established very early in the earth's history.

Schidlowski, M.↗

The Viking biology results

A brief review of the purposes and the results from the Viking Biology experiments is presented, in the expectation that the lessons learned from this mission will be useful in planning future approaches to the biological exploration of Mars. Since so little was then known about potential micro-environments on Mars, three different experiments were included in the Viking mission, each one based on different assumptions about what Martian organisms might be like. In addition to the Viking Biology Instrument (VBI), important corollary information was obtained from the Viking lander imaging system and from the molecular analysis experiments that were conducted using the gas chromatograph-mass spectrometer (GCMS) instrument. No biological objects were noted by the lander imaging instrument. The GCMS did not detect any organic compounds. A description of the tests conducted by the Gas Exchange Experiment, the Labeled Release experiment, and the Pyrolytic Release experiment is given. Results are discussed. Taken as a whole, the Viking data yielded no unequivocal evidence for a Martian biota at either landing site. The results also revealed the presence of one or more reactive oxidants in the surface material and these need to be further characterized, as does the range of micro-environments, before embarking upon future searches for extant life on Mars.

Klein, Harold P.↗

Space Biology in the 21st century

Space Biology is poised to make significant contributions to science in the next century. A carefully crafted, but largely ground-based, program in the United States has evolved major questions that require answers through experiments in space. Science, scientists, and the new long-term spacecrafts designed by NASA will be available for the first time to mount a serious Space Biology effort. The scientific challenge is of such importance that success will provide countless benefits to biologically dependent areas such as medicine, food, and commerce in the decades ahead. The international community is rapidly expanding its role in this field. The United States should generate the resources that will allow progress in Space Biology to match the recognized progress made in aeronautics and the other space sciences.

Halstead, Thora W.↗

The 1992-1993 NASA Space Biology Accomplishments

This report consists of individual technical summaries of research projects of NASA's Space Biology Program, for research conducted during the calendar years of 1992 and 1993. This program includes both plant and animal research, and is dedicated to understanding the role of gravity and the effects of microgravity on biological processes; determining the effects of the interaction of gravity and other environmental factors on biological systems; and using the microgravity of the space environment as a tool to advance fundamental scientific knowledge in the biological sciences to improve the quality of life on Earth and contribute to NASA's goal of manned exploration of space. The summaries for each project include a description of the research, a list of the accomplishments, an explanation of the significance of the accomplishments, and a list of publications.

Halstead, Thora W.↗

Aerospace Medicine and Biology: A Continuing Bibliography

This supplemental issue of Aerospace Medicine and Biology, A Continuing Bibliography with Indexes lists reports, articles, and other documents recently announced in the NASA STI Database. In its subject coverage, Aerospace Medicine and Biology concentrates on the biological, physiological, psychological, and environmental effects to which humans are subjected during and following simulated or actual flight in the Earth's atmosphere or in interplanetary space. References describing similar effects on biological organisms of lower order are also included. Such related topics as sanitary problems, pharmacology, toxicology, safety and survival, life support systems, exobiology, and personnel factors receive appropriate attention. Applied research receives the most emphasis, but references to fundamental studies and theoretical principles related to experimental development also qualify for inclusion.

Source record↗

Aerospace Medicine and Biology: A Continuing Bibliography

This supplemental issue of Aerospace Medicine and Biology, A Continuing Bibliography with Indexes (NASA/SP-1998-7011) lists reports, articles, and other documents recently announced in the NASA STI Database. In its subject coverage, Aerospace Medicine and Biology concentrates on the biological, physiological, psychological, and environmental effects to which humans are subjected during and following simulated or actual flight in the Earth's atmosphere or in interplanetary space. References describing similar effects on biological organisms of lower order are also included. Such related topics as sanitary problems, pharmacology, toxicology, safety and survival, life support systems, exobiology, and personnel factors receive appropriate attention. Applied research receives the most emphasis, but references to fundamental studies and theoretical principles related to experimental development also qualify for inclusion. Each entry in the publication consists of a standard bibliographic citation accompanied, in most cases, by an abstract.

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