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At least 109 records · Page 6

Microbial biofilm formation and its consequences for the CELSS program

A major goal of the Controlled Ecology Life Support System (CELSS) program is to provide reliable and efficient life support systems for long-duration space flights. A principal focus of the program is on the growth of higher plants in growth chambers. These crops should be grown without the risk of damage from microbial contamination. While it is unlikely that plant pathogens will pose a risk, there are serious hazards associated with microorganisms carried in the nutrient delivery systems and in the atmosphere of the growth chamber. Our experience in surface microbiology showed that colonization of surfaces with microorganisms is extremely rapid even when the inoculum is small. After initial colonization extensive biofilms accumulate on moist surfaces. These microbial films metabolize actively and slough off continuously to the air and water. During plant growth in the CELSS program, microbial biofilms have the potential to foul sensors and to plug nutrient delivery systems. In addition both metabolic products of microbial growth and degradation products of materials being considered for use as nutrient reservoirs and for delivery are likely sources of chemicals known to adversly affect plant growth.

Mitchell, R.↗

Comparison of Long-Term Storage in Chemical Fixatives on Morphology and Anatomy of Super-Dwarf Wheat

Wheat plants (Triticum aestivum L. cv Super-Dwarf) are grown in the microgravity of space and harvested for morphological and anatomical comparison with those exposed to gravity on earth. Such plants are subjected to relatively long periods of storage in chemical fixatives. Examination, evaluation and verification that the integrity of the vascular system is maintained for extended periods of storage in fixatives are required. McDowell and Trump's [4% Formaldehyde -- 1 % Glutaraldehyde (4F: 1 G)] or Variant I [(Russian Fixative): Formalin: Acetic Acid: Alcohol] fixatives, adjusted to pH 7.2, were placed in Aclam(TM), FilmORap(TM), or FilmOSun(TM) plastic bags on April 4, 1994. Wheat seedlings were harvested on days 9, 28, and 68 and preserved in these fixatives. Subsamples of leaves and/or seeds were taken from these stocks after various times in storage, dehydrated, and embedded in Spurr's, LR White's or Unicryl resin. Semithin (1 mm) and thin (50-70 nm) sections were examined by light and transmission electron microscopy. In a few sections, we have observed a slight plasmolysis of the cytostol in leaf tissue fixed with the Variant I, but overall there seem to be no major artifacts in the anatomical structure. The plasmalemma and other organelles appeared normal in the McDowell and Trump fixative. Use of differential chromophores suggests that LR White or Unicryl resins may give greater flexibility for enzyme localizations at both the light and electron microscopical levels.

Bubenheim, David L.↗

The Astroculture (tm)-1 experiment on the USML-1 mission

Permanent human presence in space will require a life support system that minimizes athe need for resupply of consumables from Earth resources. Plants that convert radiant energy to chemical energy via photosynthesis are a key component of a bioregenerative life support system. Providing the proper root environment for plants in reduced gravity is an essential aspect of the development of facilities for growing plants in a space environment. The ASTROCULTURE(TM)-1 experiment, included in the USML-1 mission, successfully demonstrated the ability of the Wisconsin Center for Space Automation and Robotics porous tube water delivery system to control water movement through a rooting matrix in a microgravity environment.

Tibbitts, Theodore↗

Chemicals from biomass - The U.S. prospects for the turn of the century

Historically, chemicals from biomass have been and are expected to be economical in three major areas: byproducts, specialty items and polymers. Assessments of producing major chemicals from biomass in a processing plant based on the available conversion techniques indicate that they are not economically attractive, with the possible exception of conversion to ammonia and ethanol. The deterrents are the heavy capital investments, dependability of raw material supply and transportation costs for large plants, lack of operation experience, inadaptability of market variations, and competition from petroleum and coal. More importantly, it is also shown that even if chemicals from biomass were economical today, the resultant savings in petroleum would be far less than those achieved through other options available for the utilization of biomass as fuel and structural material. Thus, it is concluded that near-term research and development must be toward improved conversion processes, recovery of valuable products from waste streams at existing plants, more efficient use of biomass of energy and more efficient production of superior material products.

Sarbolouki, M. N.↗

Mars soil - A sterile regolith or a medium for plant growth?

The mineralogical composition and the physical, chemical and mechanical properties of the Mars soil have been the subject of a number of studies. Though definitive mineralogical measurements are lacking, elemental-chemical analyses and simulation experiments have indicated that clays are major components of the soil and that iron is present as adsorbed ion and as amorphous mineral coating the clay particles (Banin, 1986). Whether this soil can support plant growth or food production, utilizing conventional or advanced cultivational technologies, is a question that has not been thoroughly analyzed, but may be of importance and usefulness for the future exploration of Mars. Assuming that the proposed model of Mars soil components is valid, and drawing additional information from the analyses of the SNC meteorites believed to be ejected Mars rocks - the present contribution analyzes and evaluates the suitability of the soil as a medium for plant growth, attempting to identify the most critical limiting factors for such an undertaking and the possible remedies.

Banin, Amos↗

Foliage Plants for Improving Indoor Air Quality

NASA's research with foliage houseplants during the past 10 years has produced a new concept in indoor air quality improvement. This new and exciting technology is quite simple. Both plant leaves and roots are utilized in removing trace levels of toxic vapors from inside tightly sealed buildings. Low levels of chemicals such as carbon monoxide and formaldehyde can be removed from indoor environments by plant leaves alone, while higher concentrations of numerous toxic chemicals can be removed by filtering indoor air through the plant roots surrounded by activated carbon. The activated carbon absorbs large quantities of the toxic chemicals and retains them until the plant roots and associated microorganisms degrade and assimilate these chemicals.

Wolverton, B. C.↗

Improving Aquatic Plant Management in the California Sacramento-San Joaquin Delta

Management of aquatic weeds in complex watersheds and river systems present many challenges to assessment, planning and implementation of management practices for floating and submerged aquatic invasive plants. The Delta Region Areawide Aquatic Weed Project (DRAAWP), a USDA sponsored area-wide project, is working to enhance planning, decision-making and operational efficiency in the California Sacramento-San Joaquin Delta. Satellite and airborne remote sensing are used map (area coverage and biomass), direct operations, and assess management impacts on plant communities. Archived satellite records going are used to review results from previous climate and management events and aide in developing long-term strategies. Modeling at local and watershed scales provides insight into land-use effects on water quality. Plant growth models informed by remote sensing are being applied spatially across the Delta to balance location and type of aquatic plant, growth response to altered environments, phenology, environmental regulations, and economics in selection of management practices. Initial utilization of remote sensing tools developed for mapping of aquatic invasive weeds improved operational efficiency by focusing limited chemical use to strategic areas with high plant-control impact and incorporating mechanical harvesting when chemical use is restricted. These assessment methods provide a comprehensive and quantitative view of aquatic invasive plants communities in the California Delta, both spatial and temporal, informed by ecological understanding with the objective of improving management and assessment effectiveness.

Aquatic↗

Mapping vegetation types with the multiple spectral feature mapping algorithm in both emission and absorption

Vegetation covers a large portion of the Earth's land surface. Remotely sensing quantitative information from vegetation has proven difficult because in a broad sense, all vegetation is similar from a chemical viewpoint, and most healthy plants are green. Plant species are generally characterized by the leaf and flower or fruit morphology, not by remote sensing spectral signatures. But to the human eye, many plants show varying shades of green, so there is direct evidence for spectral differences between plant types. Quantifying these changes in a predictable manner has not been easy. The Clark spectral features mapping algorithm was applied to mapping spectral features in vegetation species.

Clark, Roger N.↗

Results of ozone measurements in Northern Germany: A case study

At most of the German ozone recording stations which have records over a sufficiently long period, the results of the summer months of 1989 showed the highest values since the beginning of the measurements. One of the reasons for this phenomenon was the high duration of sunshine in that summer; for example, in Potsdam near Berlin in May 1989 the sunshine duration was the highest in May since the beginning of the records in 1893. For that reason we selected this summer for a case study. The basis for the study was mainly the ozone measuring stations of the network of Lower Saxony and the Federal Office of Environment (Umweltbundesamt). The results of these summer measurements point to intense sources of ozone, probably in form of gaseous precursors, in the Middle German industrial areas near Leipzig and Halle and in Northwestern Czechoslovakia, with coal-mining, chemical and petrochemical industries, coking plants and others. The maps of average ozone concentrations, number or days with high ozone maxima, ozone-windroses of the stations, etc., suggest that these areas could be a main source of precursors and of photochemical ozone production in summer smog episodes in Central Europe. Stations on the North Sea coast, at which early ozone measurements were made by our institute in 1973/74 are compared with similarly located stations of the Lower Saxon network in 1989 and the results show a reversal of the ozone-windroses. In 1973/74, the highest ozone concentrations were correlated with wind directions from the sea while in 1989 these concentrations were correlated with directions from the continent. In the recent years, photochemical ozone production on the continent is probably predominant, while in former years the higher ozone content of the maritime subpolar air masses has been explained by stratospheric-tropospheric exchange.

Schmidt, Manfred↗

Observing Changing Ecological Diversity in the Anthropocene

As the world enters the Anthropocene, the planet's environment is changing rapidly, putting critical ecosystem services at risk. Understanding and forecasting how ecosystems will change over the coming decades requires understanding the sensitivity of species to environmental change. The extant distribution of species and functional groups contains valuable information about the performance of different species in different environments. However, with high rates of environmental change, information inherent in ranges of many species will disappear, since that information exists only under quasi-equilibrium conditions. The information content of distributional data obtained now is greater than data obtained in the future. New remote sensing technologies can map chemical and structural traits of plant canopies and allow inference of trait and in many cases, species ranges. Current satellite remote sensing data can only produce relatively simple classifications, but new techniques have dramatically higher biological information content.

environmental changes↗

Hydrodynamics of Two-Phase Flows through Porous Media in Microgravity: Packed Bed Reactor Experiment onboard of the International Space Station

The objective of the Packed Bed Reactor Experiment was to develop a fundamental understanding of the hydrodynamics of two-phase flow with no phase transition through porous media in microgravity. This work describes two experimental campaigns flown on the International Space Station (ISS) that were designed to achieve the objective. This work presents the results on flow patterns observed, two-phase flow pressure drop in porous media, and the impact of capillary effects on pressure drop at various gas and liquid flow rates. This work is the first to present predictive correlations of the two-phase friction factor for the different regimes identified based on the superficial liquid and gas velocity in microgravity, the first to hypothesize the different regimes based on the change in slope of the pressure gradient versus flow rate plots, the first to address the effects of the capillarity on the pressure gradient and the first to measure column holdup and assess the effective porosity based on the packing and the trapped gas bubbles. Experimental data on pressure drop in gas (N2) – liquid (water) flow show the presence of four different flow regimes in microgravity. Dispersed bubble flow (DB) and pulse (P) flow regimes are detected at high liquid flow rates, whereas at low liquid flow rates “large bubble” or elongated bubble (LB) and “gas channeling” (GC) regimes are observed. For these different flow regimes, different two-phase flow friction factor and pressure gradient correlations are presented for the first time as a function of the gas and liquid modified Reynolds numbers and Suratman number. Within the viscous-capillary (V-C) regime, it is found that the capillary contribution is the dominant force that contributes to the pressure drop for the wetting case (glass). However, for the non-wetting packing (Teflon), the viscous contribution dominates. It was found that the gas hold-up and pressure drop are functions of bed history at low liquid and gas flow rates with the magnitude of the hysteresis decreasing with increasing flow rates. PBRE-2 results show that the capillary force is a strong function of the superficial liquid velocity but is a much weaker function of the superficial gas velocity and varies inversely with the particle diameter. Within the Viscous-Capillary (V-C) regime, over 90% of the pressure gradient is attributed to the capillary contribution in the gas continuous regime. However, in the large bubble regime, the viscous and capillary contributions were comparable. After the completion of PBRE-2 with glass spherical beads, another bed packed with alumina was installed. Pressure gradient data for the alumina packed bed (PBRE-Water Recovery) were transferred to the PI and were not analyzed by the authors of this work. The Packed Bed Reactor Experiment concluded its on-orbit operation after two successful campaigns of testing in 2017 and in 2021. The flight hardware was brought back from orbit and is being reconditioned for a series of future experiments referred to as PBRE-WRS in support of water recovery (WRS). The WRS series consists of testing different two-phase fluid system components and packed beds to assess their pressure gradient characteristics, which will be used for designing packed bed reactors for various applications relevant to life support. Although there is so much relevance of the experimental results obtained from the two PBRE campaign in microgravity to life support, these findings also apply to other applications that involve two phase flow in porous medium such as fuel cells, transport of nutrient to plants in space and other chemical and materials processing that involve two phase flows. These systems operate differently in microgravity because, due to the lack of buoyancy, the density difference between the phases becomes irrelevant and no longer leads to phase separation.

Packed Bed↗

Has NO(2) in Metropolitan Areas Returned to Pre-COVID-19 Levels as Measured by OMI Satellite Data?

Nitrogen oxides (NOx = NO + NO2) are primarily emitted through the combustion of fossil fuels, such as automobiles and power plants. NOx can contribute to chemical reactions that produce ozone and fine particulate matter (PM2.5) pollution. Using tropospheric vertical column density (TVCD) NO2 data from satellite hyperspectral instruments, such as the Ozone Monitoring Instrument (OMI) on board NASA’s EOS Aura satellite, one can monitor changes in NO2 pollution during “black-swan” events, such as mobility restrictions during COVID-19 lockdowns. Our OMI team has created the Global Nitrogen Dioxide Monitoring Home Page (https://so2.gsfc.nasa.gov/no2/no2_index.html ) where we display NO2 TVCD time series, animated maps, and COVID-specific NO2 anomalies comparing to a pre-COVID five-year average for 317 cities and regions around the world. During the pandemic as a result of the widespread lockdowns, many areas saw low NO2 anomalies superimposed on the natural weather related NO2 changes, such as seasonal NO2 cycle. Has NO2 levels returned to pre-Covid-19 levels in large metropolitan areas? We analyze NO2 time series from March through September for the years 2020-2023 using as a reference the average NO2 from 2015-2019 for those months. We will present data from selected cities that represent all countries.

Keith D Evans↗

Greenhouse (III): Gas-Exchange and Seed-to-Seed Experiments on the Russian Space Station MIR and Earth-grown, Ethylene-Treated Wheat Plants

The Mir Space Station provided an outstanding opportunity to study long-term plant responses when exposed to a microgravity environment. Furthermore, if plants can be grown to maturity in a microgravity environment, they might be used in future bioregenerative life-support systems (BLSS). The primary objective of the Greenhouse experiment onboard Mir was to grow Super Dwarf and Apogee wheat through complete life cycles in microgravity; i.e., from seed-to-seed-to-seed. Additional objectives were to study chemical, biochemical, and structural changes in plant tissues as well as photosynthesis, respiration, and transpiration (evaporation of water from plants). Another major objective was to evaluate the suitability clothe facilities on Mir for advanced research with plants. The Greenhouse experiment was conducted in the Russian/Bulgarian plant growth chamber, the Svet, to which the United States added instrumentation systems to monitor changes in CO2 and water vapor caused by the plants (with four infrared gas analyzers monitoring air entering and leaving two small plastic chambers). In addition, the US instrumentation also monitored O2; air, leaf (IR), cabin pressure; photon flux; and substrate temperature and substrate moisture (16 probes in the root module). Facility modifications were first performed during the summer of 1995 during Mir 19, which began after STS-72 left Mir. Plant development was monitored by daily observations and some photographs.

Campbell, William F.↗

Gravity independence of seed-to-seed cycling in Brassica rapa

Growth of higher plants in the microgravity environment of orbital platforms has been problematic. Plants typically developed more slowly in space and often failed at the reproductive phase. Short-duration experiments on the Space Shuttle showed that early stages in the reproductive process could occur normally in microgravity, so we sought a long-duration opportunity to test gravity's role throughout the complete life cycle. During a 122-d opportunity on the Mir space station, full life cycles were completed in microgravity with Brassica rapa L. in a series of three experiments in the Svet greenhouse. Plant material was preserved in space by chemical fixation, freezing, and drying, and then compared to material preserved in the same way during a high-fidelity ground control. At sampling times 13 d after planting, plants on Mir were the same size and had the same number of flower buds as ground control plants. Following hand-pollination of the flowers by the astronaut, siliques formed. In microgravity, siliques ripened basipetally and contained smaller seeds with less than 20% of the cotyledon cells found in the seeds harvested from the ground control. Cytochemical localization of storage reserves in the mature embryos showed that starch was retained in the spaceflight material, whereas protein and lipid were the primary storage reserves in the ground control seeds. While these successful seed-to-seed cycles show that gravity is not absolutely required for any step in the plant life cycle, seed quality in Brassica is compromised by development in microgravity.

Flight Experiment↗

Response of selected plant and insect species to simulated SRM exhaust mixtures and to exhaust components from SRM fuels

The possible biologic effects of exhaust products from solid rocket motor (SRM) burns associated with the space shuttle are examined. The major components of the exhaust that might have an adverse effect on vegetation, HCl and Al2O3 are studied. Dose response curves for native and cultivated plants and selected insects exposed to simulated exhaust and component chemicals from SRM exhaust are presented. A system for dispensing and monitoring component chemicals of SRM exhaust (HCl and Al2O3) and a system for exposing test plants to simulated SRM exhaust (controlled fuel burns) are described. The effects of HCl, Al2O3, and mixtures of the two on the honeybee, the corn earworm, and the common lacewing and the effects of simulated exhaust on the honeybee are discussed.

Heck, W. W.↗

Silicon production process evaluations

Chemical engineering analysis of the HSC process (Hemlock Semiconductor Corporation) for producing silicon from dichlorosilane in a 1,000 MT/yr plant was continued. Progress and status for the chemical engineering analysis of the HSC process are reported for the primary process design engineering activities: base case conditions (85%), reaction chemistry (85%), process flow diagram (60%), material balance (60%), energy balance (30%), property data (30%), equipment design (20%) and major equipment list (10%). Engineering design of the initial distillation column (D-01, stripper column) in the process was initiated. The function of the distillation column is to remove volatile gases (such as hydrogen and nitrogen) which are dissolved in liquid chlorosilanes. Initial specifications and results for the distillation column design are reported including the variation of tray requirements (equilibrium stages) with reflux ratio for the distillation.

Source record↗

Gravity, lignification, and land plant evolution

Vascular plants began their occupation of the wetlands interfacing both terrestrial and marine environments at some point in early Paleozoic time. Chemical differences between green algae and vascular land plants are mainly related to an abundance of lignins in the land plants. Answers to questions relating to the phylogeny and adaptive significance of the lignins must depend on experiments and observations using contemporary plant material. A summary is provided of a series of such observations. It is found that the differences between modern Chlorophyta and vascular land plants cannot be explained in full on the basis of lignification alone. Nevertheless, the data point to the emergence of the primitive land populations into an oxygen-rich terrestrial world where the need for mechanical support and water conservation could be met by a single aerobic biochemical process connected to essential aromatic amino acids likely to be found in every cell

Siegel, S. M.↗

Phytometric intelligence sensors

Methods and apparatus for determining when plants require watering, and methods of attending to the watering of plants including signaling the grower that the plants are in need of hydration are provided. The novel methods include real-time measurement of plant metabolics and phytometric physiology changes of intrinsic physical or behavioral traits within the plant such as determining physiological flux measurement of enzyme flux due to environmental changes such as the wind and drought stress, soil and plant mineral deficiencies, or the interaction with a bio-control for organic disease control including, cell movement, signal transduction, internal chemical processes and external environmental processes including when plants require watering, and methods of attending to the watering of plants including signaling the grower that the plants are in need of hydration.

Seelig, Hans-Dieter↗