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At least 109 records · Page 6

Vegetation Water Content Mapping in a Diverse Agricultural Landscape: National Airborne Field Experiment 2006

Mapping land cover and vegetation characteristics on a regional scale is critical to soil moisture retrieval using microwave remote sensing. In aircraft-based experiments such as the National Airborne Field Experiment 2006 (NAFE 06), it is challenging to provide accurate high resolution vegetation information, especially on a daily basis. A technique proposed in previous studies was adapted here to the heterogenous conditions encountered in NAFE 06, which included a hydrologically complex landscape consisting of both irrigated and dryland agriculture. Using field vegetation sampling and ground-based reflectance measurements, the knowledge base for relating the Normalized Difference Water Index (NDWI) and the vegetation water content was extended to a greater diversity of agricultural crops, which included dryland and irrigated wheat, alfalfa, and canola. Critical to the generation of vegetation water content maps, the land cover for this region was determined from satellite visible/infrared imagery and ground surveys with an accuracy of 95.5% and a kappa coefficient of 0.95. The vegetation water content was estimated with a root mean square error of 0.33 kg/sq m. The results of this investigation contribute to a more robust database of global vegetation water content observations and demonstrate that the approach can be applied with high accuracy. Keywords: Vegetation, field experimentation, thematic mapper, NDWI, agriculture.

Cosh, Michael H.↗

Developing a Hail and Wind Damage Swath Event Database from Daily MODIS True Color Imagery and Storm Reports for Impact Analysis and Applications

Hail and damaging winds are two threats associated with intense and severe thunderstorms that traverse the Midwest and Great Plains during the primary growing season. In certain severe thunderstorm events, large swaths of agricultural crops are impacted, allowing the damage to be viewed from multiple satellite remote sensing platforms. Previous studies have focused on analyzing individual hail and wind damage swaths (HWDSs) using satellite remote sensing, but these swaths have never been officially archived or documented. This lack of documentation has made it difficult to analyze the spatial extent and temporal frequency of HWDSs from year to year. This study utilizes daily true color imagery from MODIS aboard NASA’s Terra and Aqua satellites and daily local storm reports from the Storm Prediction Center to build a database of HWDSs occurring in the months of May through August, for years 2000 through 2020. This database identified 1,646 HWDSs in 12 states throughout the Midwest and Great Plains, confirmed through a combination of archived severe weather warnings, radar information, and official storm reports. For each entry in the HWDS database, a geospatial outline is provided along with the most likely date of first visible damage from MODIS imagery as well as the physical characteristics and time of occurrence estimated from available warnings. This study also provides a summary of the radar characteristics for a portion of the database. This database will further the understanding of severe weather damage by hail and wind to agriculture to help understand the frequency of these events and assist in mapping the impacted areas.

Climatology↗

Development of an enzyme cocktail to bioconvert untapped starch in sweet sorghum processing by-products: Part II. Application and economic potential

Demand for bioethanol and other biochemicals produced from agricultural crops has increased. Sweet sorghum has been identified as a promising bioenergy crop as it can produce starch, sugar, and fibrous biomass. During processing, starch accumulates in the juice sediments and clarification mud. And, while currently not recovered, it can be made accessible for fermentation by enzymatic hydrolysis. The enzymatic hydrolysis of starch in sweet sorghum processing streams was investigated, followed by fermentation of the resulting glucose to ethanol. The juice sediment contained sufficient starch to increase fermentable sugars by 127%, allowing a final ethanol titer of 11.1 vol%. The economics for fermentation of only dissolved sugars (Scenario 1) versus dissolved sugars and hydrolyzed starch (Scenario 2) were compared using a technoeconomic model. Fixed capital cost was found to be similar for both scenarios. For a set harvest period, Scenario 2 could produce 20% more ethanol; however, if the distillation by-product was dried and marketed as animal feed, Scenario 1 was more cost effective. A plant producing 5.6–5.7 Mkg of fuel ethanol per year had a minimum selling price of $0.67/kg of ethanol with concurrent production of animal feed and zero cost sweet sorghum processing. Finally, even with these optimistic assumptions, the estimated production cost was higher than the current wholesale price of fuel ethanol.

09 BIOMASS FUELS↗

Assessment of the feedstock supply for siting single- and multiple-feedstock biorefineries in the USA and identification of prevalent feedstocks

An integrated multi-feedstock bioenergy (i.e., biofuel, biopower, or bioproduct) supply system has potential to reduce biomass supply system uncertainties and costs. This study identifies optimal configurations of multi-feedstock biomass-to-biorefinery supply chains and pertinent feedstock combinations based on spatial distribution of feedstock and lowest delivered cost to the biorefinery. We used the Supply Characterization Model (SCM) to allocate feedstock supplies to candidate biorefinery facilities. Model runs were performed for herbaceous energy crops, agriculture residue, and woody biomass available in 2017, 2022, 2025, and 2030 as estimated by the Policy Analysis System (POLYSYS) and Forest Sustainable and Economic Analysis Model (ForSEAM) models. Three feedstock supply scenarios were compared: (a) an herbaceous scenario: switchgrass, miscanthus, biosorghum, and corn stover; (b) a woody scenario: coppice wood, noncoppice wood, whole trees, and forestry residues, and (c) a mixed scenario: a combination of all feedstocks in herbaceous and woody scenarios. By 2030 the analyses predicted that 323, 168, and 473 biorefineries were sited in the herbaceous, woody, and mixed scenario, respectively, in the conterminous USA. Feedstock mixes supplied to the biorefineries were mostly dominated by a single feedstock. The most prominent feedstock mixes identified were: (1) switchgrass and miscanthus; (2) coppice and noncoppice wood; and (3) coppice wood, noncoppice wood, switchgrass and miscanthus. Biorefineries using multi-feedstock would be beneficial for growth of bioeconomy, however flexible and cost-effective conversion platforms should be developed to efficiently utilize multiple feedstocks. This analysis identifies biorefinery locations and feedstock supply mixes while minimizing delivered feedstock costs based on spatial and temporal feedstock availability.

09 BIOMASS FUELS↗

From subsidies to stressors: Positively skewed ecological gradients alter biological responses to nutrients in streams

Abstract Subsidy–stress gradients offer a useful framework for understanding ecological responses to perturbation and may help inform ecological metrics in highly modified systems. Historic, region‐wide shifts from bottomland hardwood forest to row crop agriculture can cause positively skewed impact gradients in alluvial plain ecoregions, resulting in tolerant organisms that typically exhibit a subsidy response (increased abundance in response to environmental stressors) shifting to a stress response (declining abundance at higher concentrations). As a result, observed biological tolerance in modified ecosystems may differ from less modified regions, creating significant challenges for detecting biological responses to restoration efforts. Using the agriculturally dominated Mississippi Alluvial Plain (MAP) ecoregion in Mississippi, USA, as a case study, we tested the hypothesis that macroinvertebrate taxa that typically display a subsidy response to nutrient enrichment in less modified ecoregions (i.e., nutrient‐tolerance) shift to a stress response to increasing nutrients in highly modified watersheds with elevated baseline nutrient conditions (i.e., nutrient intolerance). The abundance and diversity of MAP‐specific intolerant taxa identified with threshold indicator taxa analysis were either unresponsive or exhibited a subsidy response to increasing nutrients in less modified ecoregions in Mississippi with less land alteration and lower nutrient concentrations, but declined at higher concentrations, providing evidence for a stress response to elevated nutrients in the MAP. Additionally, MAP‐specific tolerant and intolerant taxa richness responded to increased nutrients predictably and consistently across space and time within the MAP. However, in MAP streams, elevated specific conductance was predicted to dampen the response of tolerant and intolerant taxa richness to increasing nutrient concentrations, highlighting the importance of considering multistressor interactions when interpreting biological data. Lastly, we demonstrate the efficacy of this approach with sediment bacterial communities characterized with amplicon sequencing, which lack sufficient life history characteristics necessary for the development of multimetric indices. Both macroinvertebrate and bacterial communities responded similarly to increasing nutrient concentrations, suggesting DNA‐based approaches may provide an efficient biological assessment tool for monitoring water quality improvements in highly modified watersheds.

DeVilbiss, Stephen E. [U.S. Geological Survey Lowe↗

Voxel carving–based 3D reconstruction of sorghum identifies genetic determinants of light interception efficiency

Changes in canopy architecture traits have been shown to contribute to yield increases. Optimizing both light interception and light interception efficiency of agricultural crop canopies will be essential to meeting the growing food needs. Canopy architecture is inherently three-dimensional (3D), but many approaches to measuring canopy architecture component traits treat the canopy as a two-dimensional (2D) structure to make large scale measurement, selective breeding, and gene identification logistically feasible. We develop a high throughput voxel carving strategy to reconstruct 3D representations of sorghum from a small number of RGB photos. Our approach builds on the voxel carving algorithm to allow for fully automatic reconstruction of hundreds of plants. It was employed to generate 3D reconstructions of individual plants within a sorghum association population at the late vegetative stage of development. Light interception parameters estimated from these reconstructions enabled the identification of known and previously unreported loci controlling light interception efficiency in sorghum. The approach is generalizable and scalable, and it enables 3D reconstructions from existing plant high throughput phenotyping datasets. We also propose a set of best practices to increase 3D reconstructions’ accuracy.

3D plant reconstruction↗

Multiplex Editing of the Nucleoredoxin1 Tandem Array in Poplar: From Small Indels to Translocations and Complex Inversions

The CRISPR-Cas9 system has been deployed for precision mutagenesis in an ever-growing number of species, including agricultural crops and forest trees. Its application to closely linked genes with extremely high sequence similarities has been less explored. In this study, we used CRISPR-Cas9 to mutagenize a tandem array of seven Nucleoredoxin1 (NRX1) genes spanning ~100 kb in Populus tremula × Populus alba. We demonstrated efficient multiplex editing with one single guide RNA in 42 transgenic lines. The mutation profiles ranged from small insertions and deletions and local deletions in individual genes to large genomic dropouts and rearrangements spanning tandem genes. We also detected complex rearrangements including translocations and inversions resulting from multiple cleavage and repair events. Target capture sequencing was instrumental for unbiased assessments of repair outcomes to reconstruct unusual mutant alleles. The work highlights the power of CRISPR-Cas9 for multiplex editing of tandemly duplicated genes to generate diverse mutants with structural and copy number variations to aid future functional characterization.

59 BASIC BIOLOGICAL SCIENCES↗

Ecovoltaic solar energy development can promote grassland bird communities

Ecologically informed photovoltaic (PV) developments that co-prioritize PV electricity generation with ecosystem function (‘ecovoltaics’) have emerged as a promising land sharing strategy to minimize ecological conflicts associated with PV solar energy development. While habitat-focused ecovoltaic designs can conceptually benefit biodiversity by offsetting or enhancing impacts of PV development, foundational field research is needed to examine how wildlife respond to these novel ecosystems. We conducted passive acoustic monitoring (PAM) in 2023 and 2024 at 13 solar facilities and paired control sites to investigate avian community responses to ecovoltaic facilities in the Midwestern United States. Compared to control sites (row crop agricultural fields), we found that ecovoltaic sites supported more grassland bird species throughout a 17-week monitoring period between May and September. Grassland bird communities on ecovoltaic sites were also more stable than on agricultural controls, as measured by the Jaccard dissimilarity index. We also used PAM-based weekly species occurrences in an occupancy-modelling framework to investigate the influence of PV development and other landscape variables on grassland bird occupancy. 10 out of 13 modelled grassland bird species had greater predicted occupancy probabilities (ψ) on PV sites than control sites. Synthesis and applications. Our findings suggest that properly sited and developed ecovoltaic solar facilities in human altered landscapes can improve habitat for birds and other wildlife, but further research is needed to understand which species may benefit most from these novel ecosystems.

14 SOLAR ENERGY↗

Engineered Methylobacterium extorquens grows well on methoxylated aromatics due to its formaldehyde metabolism and stress response

ABSTRACT Lignin is a vast yet underutilized source of renewable energy. The microbial valorization of lignin is challenging due to the toxicity of its degradation intermediates, particularly formaldehyde. In this study, we engineeredMethylobacterium extorquensPA1 to metabolize lignin-derived methoxylated aromatics, vanillate (VA) and protocatechuate (PCA), by introducing thevanandpcagene clusters. Compared toPseudomonas putida,M. extorquensPA1 exhibited better formaldehyde detoxification, enabling robust growth on VA without accumulation of formaldehyde. Genetic analyses confirmed that formaldehyde oxidation and stress response systems, rather than C 1 assimilation, were important for VA metabolism. Additionally, VA and PCA were found to disrupt membrane potential, contributing to their inherent toxicity. Our findings establishM. extorquensPA1 as a promising chassis for lignin valorization and provide a framework for engineering formaldehyde-resistant microbial platforms. IMPORTANCE In developing biotechnological solutions for a circular economy, it is critical to valorize all parts of renewable resources, such as lignocellulose from vegetative components of agricultural crops and from bioenergy feedstocks. After chemical breakdown, the aromatics arising from lignin present significant challenges for use due to their toxicity. Here, we address one component of this challenge—the methoxy groups that get released as formaldehyde—and show that existing biotechnological platform organisms with strong formaldehyde metabolism, such asMethylobacterium extorquens, can be transformed into highly capable utilizers of methoxylated aromatics.

Microbiology↗

AmeriFlux US-KL2 KBS Lux Arbor Reserve Switchgrass

This is the AmeriFlux version of the carbon flux data for the site US-KL2 KBS Lux Arbor Reserve Switchgrass. Site Description - The site was under conventional corn/soybean rotation row crop agriculture for decades before conversion. It was converted to no-till soybean in 2009 and to perennial switchgrass from 2010 onwards. Starting from 2011, the switchgrass is harvested every autumn/fall.

Robertson, G. Philip↗

AmeriFlux US-KL3 KBS Lux Arbor Reserve Prairie

This is the AmeriFlux version of the carbon flux data for the site US-KL3 KBS Lux Arbor Reserve Prairie. Site Description - The site was under conventional corn/soybean rotation row crop agriculture for decades before conversion. The site was converted to no-till soybean in 2009 and to perennial native prairie from 2010 onwards. Starting from 2011, the prairie is harvested every autumn/fall.

Robertson, G. Philip↗

AmeriFlux US-KL1 KBS Lux Arbor Reserve Corn

This is the AmeriFlux version of the carbon flux data for the site US-KL1 KBS Lux Arbor Reserve Corn. Site Description - The site was under conventional corn/soybean rotation row crop agriculture for decades before conversion. The site was converted to no-till soybean in 2009 and to no-till continuous corn from 2010 onwards.

Robertson, G. Philip↗

Chimeric Protein Treatment of Pierce Disease

Pierce disease is an economically costly disease for several agricultural crops, including grapes, citrus, and olives. An invasive insect species, the glassy-winged sharpshooter, harbors a gut bacteria that kills grape vines. The bacteria destroys the xylem, the water transport tissue in plants. Los Alamos researchers have designed a technology to target and kill the source of Pierce disease. The treatment does not require the use of pesticides or the removal and replacement of the effected vines.

59 BASIC BIOLOGICAL SCIENCES↗

Raman Spectroscopy to Detect and Measure NOW Pheromones. Annual Report [Jan 2021]

Insect sex pheromones are chemical compounds that insects release to attract their partners over distances of hundreds of meters or even kilometers, in complete darkness and without any audible signals. Use of synthetic forms of key compounds have in some cases become an essential component of monitoring and/or managing key pests of agricultural crops, including navel orangeworm (Amyelois transitella) (NOW) in California tree nuts. These pheromone-based strategies can include monitoring, mating disruption, mass trapping, attract-and-kill and push-pull. There are currently multiple commercially available mating disruption products available for NOW and recent studies have demonstrated that they can be effectively used to reduce crop damage by this pest. Just how mating disruption works is not fully established and likely varies across products and target species. For instance, the extent to which synthetic pheromones compete with natural pheromone is not well understood, or in the case of monitoring, how efficaciously the insect follows the diffusing plumes, especially across large blocks and at plot borders. Furthermore, there may be specific conditions under which poor or impeded diffusion of synthetic pheromone diminishes the disruption effect, which could result in some males effectively locating females for reproduction. At the same time, many commercially available synthetic lures are also available, and growers have been effectively using them to track population development – although the attractive range of these lures remains unclear. That is, while pheromone lures can attract many moths, the relationship between trap capture and local populations, much less crop damage, remains unclear.

47 OTHER INSTRUMENTATION↗

Raman Spectroscopy to Detect and Measure NOW Pheromones. Annual Report [2021]

Insect sex pheromones are chemical compounds that insects release to attract mates over distances of hundreds of meters or even kilometers, in complete darkness and without any audible signals. Use of synthetic forms of key compounds have in some cases become an essential component of monitoring and/or managing key pests of agricultural crops, including navel orangeworm (Amyelois transitella) (NOW) in California tree nuts There are currently multiple commercially available mating disruption products available for NOW and recent studies have demonstrated that they can be effectively used to reduce crop damage. How mating disruption works is not fully established and likely varies across products and target species. For instance, it is not well understood how synthetic pheromones compete with natural pheromones and, in the case of monitoring, how efficaciously the insect follows the diffusing plumes and how those evolve from emission points, especially across large blocks and at plot borders. We proposed to evaluate the use of enhanced Raman spectroscopy for the detection of synthetic and natural NOW pheromones and, were that to be successful, use it in subsequent years to measure pheromone diffusion in orchards.

47 OTHER INSTRUMENTATION↗

Raman Spectroscopy to Detect and Measure NOW Pheromones. Annual Report [Dec 2021]

Insect sex pheromones are chemical compounds that insects release to attract mates over distances of hundreds of meters or even kilometers, in complete darkness and without any audible signals. Use of synthetic forms of key compounds have in some cases become an essential component of monitoring and/or managing key pests of agricultural crops, including navel orangeworm (Amyelois transitella) (NOW) in California tree nuts There are currently multiple commercially available mating disruption products available for NOW and recent studies have demonstrated that they can be effectively used to reduce crop damage. How mating disruption works is not fully established and likely varies across products and target species. For instance, it is not well understood how synthetic pheromones compete with natural pheromones and, in the case of monitoring, how efficaciously the insect follows the diffusing plumes and how those evolve from emission points, especially across large blocks and at plot borders. We proposed to evaluate the use of enhanced Raman spectroscopy for the detection of synthetic and natural NOW pheromones and, were that to be successful, use it in subsequent years to measure pheromone diffusion in orchards.

47 OTHER INSTRUMENTATION↗

Raman Spectroscopy to Detect and Measure NOW Pheromones

Insect sex pheromones are chemical compounds that insects release to attract mates over distances of hundreds of meters or even kilometers, in complete darkness and without any audible signals. Use of synthetic forms of key compounds have in some cases become an essential component of monitoring and/or managing key pests of agricultural crops, including navel orangeworm (Amyelois transitella) (NOW) in California tree nuts. There are currently multiple commercially available mating disruption products available for NOW and recent studies have demonstrated that they can effectively help reducing crop damage. How mating disruption works is not fully established and likely varies across products and target species. For instance, it is not well understood how synthetic pheromones compete with natural ones and, in the case of monitoring, how efficaciously the insect follows the diffusing plumes and how those evolve from emission points, especially across large blocks and at plot borders.

47 OTHER INSTRUMENTATION↗

Raman Spectroscopy to Detect and Measure NOW Pheromones ("Orchard in a box")

Insect sex pheromones are chemical compounds that insects release to attract mates over distances of hundreds of meters or even kilometers, in complete darkness and without any audible signals. Use of synthetic forms of key compounds have in some cases become an essential component of monitoring and/or managing key pests of agricultural crops, including navel orangeworm (Amyelois transitella) (NOW) in California tree nuts. There are currently multiple commercially available mating disruption products available for NOW and recent studies have demonstrated that they can be effectively used to reduce crop damage. How mating disruption works is not fully established and likely varies across products and target species. For instance, it is not well understood how synthetic pheromones compete with natural pheromones and, in the case of monitoring, how efficaciously the insect follows the diffusing plumes, especially across large blocks and at plot borders. Whether an aerosol puffer is used for mating disruption or lure for monitoring population development, we currently lack the ability to fully understand how these synthetic pheromone compounds diffuse away from their point-source of emission – and subsequently how this might affect the efficacy of mating disruption and/or the accuracy of monitoring efforts. In response to these unknowns, Raman spectroscopy may offer a way to detect synthetic pheromone because of its analytical properties and recent technological advancements. In combination with nanostructured probes, RS has demonstrated the capability to detect volatiles at extremely low concentrations. We proposed to evaluate the use of Raman for the detection of synthetic and natural NOW pheromones and, were that to be successful, use this tool in subsequent years to measure pheromone diffusion in orchards.

47 OTHER INSTRUMENTATION↗