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At least 109 records · Page 6

Genomic and Phenotypic Characterization of Yeast Biosensor for Deep-space Radiation

The BioSentinel mission was selected to launch as a secondary payload onboard NASA Exploration Mission 1 (EM-1) in 2018. In BioSentinel, the budding yeast Saccharomyces cerevisiae will be used as a biosensor to measure the long-term impact of deep-space radiation to living organisms. In the 4U-payload, desiccated yeast cells from different strains will be stored inside microfluidic cards equipped with 3-color LED optical detection system to monitor cell growth and metabolic activity. At different times throughout the 12-month mission, these cards will be filled with liquid yeast growth media to rehydrate and grow the desiccated cells. The growth and metabolic rates of wild-type and radiation-sensitive strains in deep-space radiation environment will be compared to the rates measured in the ground- and microgravity-control units. These rates will also be correlated with measurements obtained from onboard physical dosimeters. In our preliminary long-term desiccation study, we found that air-drying yeast cells in 10% trehalose is the best method of cell preservation in order to survive the entire 18-month mission duration (6-month pre-launch plus 12-month full-mission periods). However, our study also revealed that desiccated yeast cells have decreasing viability over time when stored in payload-like environment. This suggests that the yeast biosensor will have different population of cells at different time points during the long-term mission. In this study, we are characterizing genomic and phenotypic changes in our yeast biosensor due to long-term storage and desiccation. For each yeast strain that will be part of the biosensor, several clones were reisolated after long-term storage by desiccation. These clones were compared to their respective original isolate in terms of genomic composition, desiccation tolerance and radiation sensitivity. Interestingly, clones from a radiation-sensitive mutant have better desiccation tolerance compared to their original isolate without losing radiation sensitivity. We employed Next-Generation Sequencing technology to better understand this phenotypic variation. Current effort is focusing on the analysis of high-throughput sequencing data to look for genomic changes in these reisolated clones compared to their original isolate.

yeast↗

Impact-driven mobilization of deep crustal brines on dwarf planet Ceres

Ceres, the only dwarf planet in the inner Solar System, appears to be a relict ocean world. Data collected by NASA's Dawn spacecraft provided evidence that global aqueous alteration within Ceres resulted in a chemically evolved body that remains volatile-rich(1). Recent emplacement of bright deposits sourced from brines attests to Ceres being a persistently geologically active world(2,3), but the surprising longevity of this activity at the 92-km Occator crater has yet to be explained. Here, we use new high-resolution Dawn gravity data to study the subsurface architecture of the region surrounding Occator crater, which hosts extensive young bright carbonate deposits (faculae). Gravity data and thermal modelling imply an extensive deep brine reservoir beneath Occator, which we argue could have been mobilized by the heating and deep fracturing associated with the Occator impact, leading to long-lived extrusion of brines and formation of the faculae. Moreover, we find that pre-existing tectonic cracks may provide pathways for deep brines to migrate within the crust, extending the regions affected by impacts and creating compositional heterogeneity. The long-lived hydrological system resulting from the impact might also occur for large impacts in icy moons, with implications for creation of transient habitable niches over time.High-resolution data of Ceres's bright spots (faculae), obtained by Dawn's second extended mission, suggest the existence of a deep brine-rich reservoir that emerged to the surface through long-lived cryovolcanic activity as a consequence of the impact that created Occator crater.

C. A. Raymond↗

EPOXI Trajectory and Maneuver Analyses

The EPOXI mission is a NASA Discovery Mission of Opportunity combining two separate investigations: Extrasolar Planet Observation and Characterization (EPOCh) and Deep Impact eXtended Investigation (DIXI). Both investigations reused the DI instruments and spacecraft that successfully flew by the comet Tempel-1 (4 July 2005). For EPOCh, the goal was to find exoplanets with the high resolution imager, while for DIXI it was to fly by the comet Hartley 2 (4 Nov 2010). This paper documents the navigation experience of the earlier ma-neuver analyses critical for the EPOXI mission including statistical ?V analyses and other useful analyses in designing maneuvers. It also recounts the trajectory design leading up to the final reference trajectory to Hartley 2.

trajectory design↗

Direct Drive Hall Thruster System Development

The sta:us of development of a Direct Drive Ha!! Thruster System is presented. 13 the first part. a s:udy of the impacts to spacecraft systems and mass benefits of a direct-drive architecture is reviewed. The study initially examines four cases of SPT-100 and BPT-4000 Hall thrusters used for north-south station keeping on an EXPRESS-like geosynchronous spacecraft and for primary propulsion for a Deep Space- 1 based science spacecraft. The study is also extended the impact of direct drive on orbit raising for higher power geosynchronous spacecraft and on other deep space missions as a function of power and delta velocity. The major system considerations for accommodating a direct drive Hall thruster are discussed, including array regulation, system grounding, distribution of power to the spacecraft bus, and interactions between current-voltage characteristics for the arrays and thrusters. The mass benefit analysis shows that, for the initial cases, up to 42 kg of dry mass savings is attributable directly to changes in the propulsion hardware. When projected mass impacts of operating the arrays and the electric power system at 300V are included, up to 63 kg is saved for the four initial cases. Adoption of high voltage lithium ion battery technology is projected to further improve these savings. Orbit raising of higher powered geosynchronous spacecraft, is the mission for which direct drive provides the most benefit, allowing higher efficiency electric orbit raising to be accomplished in a limited period of time, as well as nearly eliminating significant power processing heat rejection mass. The total increase in useful payload to orbit ranges up to 278 kg for a 25 kW spacecraft, launched from an Atlas IIA. For deep space missions, direct drive is found to be most applicable to higher power missions with delta velocities up to several km/s , typical of several Discovery-class missions. In the second part, the status of development of direct drive propulsion power electronics is presented. The core of this hardware is the heater-keeper-magnet supply being qualified for the BPT-4000 by Aerojet. A breadboard propulsion power unit is in fabrication and is scheduled for delivery late in 2003.

Hoskins, W. Andrew↗

Attitude Determination and Control Subsystem (ADCS) Preparations for the EPOXI Flyby of Comet Haley 2

On November 4, 2010 the already "in-flight" Deep Impact spacecraft flew within 700km of comet 103P/Hartley 2 as part of its extended mission EPOXI, the 5th time to date any spacecraft visited a comet. In 2005, the spacecraft had previously imaged a probe impact comet Tempel 1. The EPOXI flyby marked the first time in history that two comets were explored with the same instruments on a re-used spacecraft-with hardware and software originally designed and optimized for a different mission. This made the function of the attitude determination and control subsystem (ADCS) critical to the successful execution of the EPOXI flyby. As part of the spacecraft team preparations, the ADCS team had to perform thorough sequence reviews, key spacecraft activities and onboard calibrations. These activities included: review of background sequences for the initial conditions vector, sun sensor coefficients, and reaction wheel assembly (RWA) de-saturations; design and execution of 10 trajectory correction maneuvers; science calibration of the two telescope instruments; a flight demonstration of the fastest turns conducted by the spacecraft between Earth and comet point; and assessment of RWA health (given RWA problems on other spacecraft).

Harley↗

Space Algae: Understanding the Genomic Impacts on Microalgae After Growth in the International Space Station

Plants and microbes can be used for biological support of crewed space missions. The radiation and microgravity environment of spaceflight is expected to increase genetic mutation of all organisms. It is essential to understand how spaceflight impacts mutation rates in photosynthetic organisms to enable appropriate countermeasures and ensure productivity during long duration and deep space missions. The Space Algae flight experiments to the International Space Station (ISS) are studying the genomic stability of microalgae that could potentially be used in biological life support systems. Space Algae-1 grew ultraviolet light mutagenized Chlamydomonas reinhardtii in the VEGGIE plant growth chamber for approximately 40 mitotic generations over one month on the ISS. Whole genome sequencing from pooled cell samples every 10 generations revealed that spaceflight cultures had an ~50% increase in DNA polymorphisms relative to ground controls. These mutations had a novel base substitution signature and suggested a risk that microalgae may be unstable for long-term production in space. Space Algae-2 is focusing on the edible cyanobacterium Arthrospira platensis, commonly known as Spirulina. This experiment seeks to grow serial cultures to allow the organism to evolve in long-term spaceflight. Biological responses of the cells to spaceflight will be assessed with multi-omics analyses to determine mutation load, gene/protein expression, metabolic/nutritional composition, and cell morphology.

Algae↗

Space Algae: Understanding the Genomic Impacts on Microalgae After Growth in the International Space Station

Plants and microbes can be used for biological support of crewed space missions. The radiation and microgravity environment of spaceflight is expected to increase genetic mutation of all organisms. It is essential to understand how spaceflight impacts mutation rates in photosynthetic organisms to enable appropriate countermeasures and ensure productivity during long duration and deep space missions. The Space Algae flight experiments to the International Space Station (ISS) are studying the genomic stability of microalgae that could potentially be used in biological life support systems. Space Algae-1 grew ultraviolet light mutagenized Chlamydomonas reinhardtii in the VEGGIE plant growth chamber for approximately 40 mitotic generations over one month on the ISS. Whole genome sequencing from pooled cell samples every 10 generations revealed that spaceflight cultures had an ~50% increase in DNA polymorphisms relative to ground controls. These mutations had a novel base substitution signature and suggested a risk that microalgae may be unstable for long-term production in space. Space Algae-2 is focusing on the edible cyanobacterium Arthrospira platensis, commonly known as Spirulina. This experiment seeks to grow serial cultures to allow the organism to evolve in long-term spaceflight. Biological responses of the cells to spaceflight will be assessed with multi-omics analyses to determine mutation load, gene/protein expression, metabolic/nutritional composition, and cell morphology.

Algae↗

A Dedicated, Long Duration Balloon Mission from Antarctica to Measure the Effects of Low Dose Galactic Cosmic Radiation on Biology

Antarctic long duration balloon missions flown by NASA’s Science Mission Directorate (SMD)can be used as a surrogate for the deep space radiation environment, reducing the need to launch orbital experiments to assess the impact of galactic cosmic radiation (GCR) on biology. To date, over fifty NASA balloon missions flown from Antarctica have carried scientific payloads from Astrophysics (APD) and Heliophysics (HPD)in SMD. Only two life science experiments have been flown from Antarctica, and both were ride-along (piggyback) opportunities, limiting the sophistication and types of model organisms that can be incorporated into studies. Herein, we argue for establishing a large, dedicated Antarctic balloon mission for the Biological and Physical Sciences (BPS) Division in SMD to be launched in 2029/2030, with an “omnibus” gondola carrying dozens of independent Space Biology payloads that would receive a sustained exposure to low dose rate GCRs for 30+ days. Our unprecedented, protracted radiation experiment cannot be done using ground-based simulation facilities or in space; it can only be achieved through an Antarctic balloon mission dedicated to BPS Division payloads. By providing more access to radiation research platforms through existing NASA SMD access to Antarctic balloon flight opportunities, the Space Biology community will be better positioned to address unknowns associated with low dose rate GCR exposures in long duration spaceflight.

David J Smith↗

BioSentinel: NASA’s First Deep Space Biological Mission

Since Apollo 17 in 1972, NASA has sent no humans or other biological organisms outside of Earth’s protective magnetosphere. NASA’s current Artemis program plans to put astronauts back on the Moon and eventually land human missions on Mars. One of the major challenges to long-duration crewed travel and habitation in deep space is an in-depth understanding of the biological effects of space radiation, often convoluted by the impact of reduced gravity. Such missions will require significant countermeasures, likely both technological and biomedical, to protect organisms from chronic radiation exposure. Small satellite missions like CubeSats can inform these countermeasures by investigating model organisms in relevant space environments. The BioSentinel mission is comprised of four segments developed at NASA Ames Research Center: a 6U CubeSat (1U = 10-cm cube), an ISS payload launched in December 2021 and two ground units, one for the mission’s CubeSat and one for the ISS payload. The last three segments have been operational since January 2022 and serve as experimental controls. BioSentinel’s 6U CubeSat is planned to launch as a secondary payload on the Artemis-1 rocket. It will be deployed on a lunar fly-by trajectory and into a heliocentric orbit. BioSentinel will be the first interplanetary satellite to study the biological response to space radiation outside Low Earth Orbit (LEO) in almost 50 years. BioSentinel is a complete, autonomous spacecraft capable of conducting experiments in deep space. Its 4U BioSensor payload is a fully automated and adaptable platform that can perform biological measurements with a range of microorganisms in multiple space environments, including the ISS, free flyers, and other platforms like the Lunar Gateway and lander vehicles. Once it reaches its orbit, BioSentinel’s CubeSat will measure the DNA damage response to ambient radiation in a model organism, the budding yeast Saccharomyces cerevisiae, which will be compared to information provided by an onboard radiation sensor and to data obtained in LEO (on ISS) and on Earth. Once in interplanetary space, fluidic cards containing desiccated yeast will be activated by growth medium addition at different time points throughout the mission. Growth and metabolic activity will be tracked continuously via optical measurements. This paper describes BioSentinel’s objectives, science, data management, and preliminary results from the ISS and ISS ground control segments.

BioSentinel↗

BioSentinel: NASA’s First Deep Space Biological Mission

Since Apollo 17 in 1972, NASA has sent no humans or other biological organisms outside of Earth’s protective magnetosphere. NASA’s current Artemis program plans to put astronauts back on the Moon and eventually land human missions on Mars. One of the major challenges to long-duration crewed travel and habitation in deep space is an in-depth understanding of the biological effects of space radiation, often convoluted by the impact of reduced gravity. Such missions will require significant countermeasures, likely both technological and biomedical, to protect organisms from chronic radiation exposure. Small satellite missions like CubeSats can inform these countermeasures by investigating model organisms in relevant space environments. The BioSentinel mission is comprised of four segments developed at NASA Ames Research Center: a 6U CubeSat (1U = 10-cm cube), an ISS payload launched in December 2021 and two ground units, one for the mission’s CubeSat and one for the ISS payload. The last three segments have been operational since January 2022 and serve as experimental controls. BioSentinel’s 6U CubeSat is planned to launch as a secondary payload on the Artemis-1 rocket. It will be deployed on a lunar fly-by trajectory and into a heliocentric orbit. BioSentinel will be the first interplanetary satellite to study the biological response to space radiation outside Low Earth Orbit (LEO) in almost 50 years. BioSentinel is a complete, autonomous spacecraft capable of conducting experiments in deep space. Its 4U BioSensor payload is a fully automated and adaptable platform that can perform biological measurements with a range of microorganisms in multiple space environments, including the ISS, free flyers, and other platforms like the Lunar Gateway and lander vehicles. Once it reaches its orbit, BioSentinel’s CubeSat will measure the DNA damage response to ambient radiation in a model organism, the budding yeast Saccharomyces cerevisiae, which will be compared to information provided by an onboard radiation sensor and to data obtained in LEO (on ISS) and on Earth. Once in interplanetary space, fluidic cards containing desiccated yeast will be activated by growth medium addition at different time points throughout the mission. Growth and metabolic activity will be tracked continuously via optical measurements. This paper describes BioSentinel’s objectives, science, data management, and preliminary results from the ISS segment.

BioSentinel↗

Results from the NASA Spacecraft Fault Management Workshop: Cost Drivers for Deep Space Missions

Fault Management, the detection of and response to in-flight anomalies, is a critical aspect of deep-space missions. Fault management capabilities are commonly distributed across flight and ground subsystems, impacting hardware, software, and mission operations designs. The National Aeronautics and Space Administration (NASA) Discovery & New Frontiers (D&NF) Program Office at Marshall Space Flight Center (MSFC) recently studied cost overruns and schedule delays for five missions. The goal was to identify the underlying causes for the overruns and delays, and to develop practical mitigations to assist the D&NF projects in identifying potential risks and controlling the associated impacts to proposed mission costs and schedules. The study found that four out of the five missions studied had significant overruns due to underestimating the complexity and support requirements for fault management. As a result of this and other recent experiences, the NASA Science Mission Directorate (SMD) Planetary Science Division (PSD) commissioned a workshop to bring together invited participants across government, industry, and academia to assess the state of the art in fault management practice and research, identify current and potential issues, and make recommendations for addressing these issues. The workshop was held in New Orleans in April of 2008. The workshop concluded that fault management is not being limited by technology, but rather by a lack of emphasis and discipline in both the engineering and programmatic dimensions. Some of the areas cited in the findings include different, conflicting, and changing institutional goals and risk postures; unclear ownership of end-to-end fault management engineering; inadequate understanding of the impact of mission-level requirements on fault management complexity; and practices, processes, and tools that have not kept pace with the increasing complexity of mission requirements and spacecraft systems. This paper summarizes the findings and recommendations from that workshop, particularly as fault management development issues affect operations and the development of operations capabilities.

Newhouse, Marilyn E.↗

The U.S. Rosetta Project: Mars Gravity Assist

Since launch on March 2, 2004, the International Rosetta Mission has flown by the Earth/Moon system one time and conducted several distant observations of comets, including support for the Deep Impact measurements of comet 9 P/Tempel 1. In 2007, Rosetta flew by Mars for a gravity assist, and conducted observations of the Martian upper atmosphere as well as extended observations, in support of the New Horizons Jupiter encounter, of the Jovian magnetotail and Io torus. In late 2007 Rosetta had its second encounter with the Earth/Moon system. NASA's contribution to the Rosetta mission consists of three hardware experiments, and the portion of the electronics package for a fourth, as well as the participation of an Interdisciplinary Scientist (IDS); backup tracking, telecommunications, and navigation assurance provided by the Deep Space Network (DSN); support for the scientific participation of U.S. investigators on non-U.S. PI-led experiments. Collectively these elements are known as the U.S. Rosetta Project. In this paper we will update the status of the instruments following the both the Mars and Earth/Moon gravity assists. In addition, we will present a summary of the science observations for both Mars and Jupiter. 12.

Rosetta↗

Using Reinforcement Learning to Design Missed Thrust Resilient Trajectories

From ion thrusters to solar sails, spacecraft continue to adopt new and more efficient forms of propulsion. As these low-thrust propulsion meth- ods have become more prevalent, new challenges have arisen. Depending on the mission, low-thrust propulsion elements may need to thrust con- tinuously for days/months. During these thrusting periods, external fac- tors, such as a micro-meteoroid impact or a software glitch, may cause the spacecraft to prematurely cease its thrust stage. Half of all deep space missions enter a safe mode where they cannot thrust every four months. These missed thrust events can result in the complete loss of a space- craft for time-dependent trajectories like planetary rendezvous. This paper demonstrates how neural networks, trained using reinforcement learning, can autonomously correct for missed thrust events during an interplanetary trajectory.

Laipert, Frank E.↗

Understanding the Impacts of Deep Space Environment on Crop Production

NASA’s goal of developing sustainable habitats to support long duration, deep space missions requires advanced science, technology, and engineering. Understanding the integrated, long-term effects of deep space environments on biological systems is needed. Sustainable habitats require the production of food and oxygen on site.

Bruce M Link↗

Throttling Impacts on Hall Thruster Performance, Erosion, and Qualification for NASA Science Missions

With the SMART-1, Department of Defense, and commercial industry successes in Hall thruster technologies, NASA has started considering Hall thrusters for science missions. The recent Discovery proposals included a Hall thruster science mission and the In-Space Propulsion Project is investing in Hall thruster technologies. As the confidence in Hall thrusters improve, ambitious multi-thruster missions are being considered. Science missions often require large throttling ranges due to the 1/r(sup 2) power drop-off from the sun. Deep throttling of Hall thrusters will impact the overall system performance. Also, Hall thrusters can be throttled with both current and voltage, impacting erosion rates and performance. Last, electric propulsion thruster lifetime qualification has previously been conducted with long duration full power tests. Full power tests may not be appropriate for NASA science missions, and a combination of lifetime testing at various power levels with sufficient analysis is recommended. Analyses of various science missions and throttling schemes using the Aerojet BPT-4000 and NASA 103M HiVHAC thruster are presented.

Dankanich, John W.↗

Numerical Modeling of the South Pole-Aitkin Impact

The South Pole-Aitkin (SPA) basin, on the far side of the Moon, is the largest and oldest impact structure still preserved in the solar system. The crater is about 2500 km in diameter and formed in the Pre-Nectarian era of lunar history, over 4 Gyr ago. At this time, the thermal state of the Moon was much hotter than it is today. Accretional energy from the rapidly forming Moon melted the outermost few hundred kilometers of the Moon. As this magma ocean differentiated and cooled a 60 100-km thick low-density crust formed at the surface; below this the residual melt, with a higher density, cooled to form the lunar mantle. The giant SPA impact event punctured the Moon some time during the cooling of the magma ocean and thus provides a unique window for studying the lunar interior and the early formative processes of the Moon. The impact excavated otherwise inaccessible samples of the deep crust and (possibly) upper mantle, which has inspired proposed sample return missions. Furthermore, the thermal and rheologic state of the early Moon played a role in shaping the final structure of the basin. To aid in site selection for future sample return missions to the SPA basin, and to investigate the effect of thermal state on final crater structure, we performed some numerical simulations of the SPA impact event.

Collins, G. S.↗

The Use of Standards on the LADEE Mission

The Lunar Atmosphere Dust Environment Explorer (LADEE) was a small explorer class mission that launched Sept 7, 2013 and successfully de-orbited and impacted the moon's surface on April 17, 2014. The spacecraft was the first to launch from a Minotaur 5 and was the first deep space mission to launch from the Wallops flight facility. Figure 1 shows the famous image of a frog unlucky enough to be launched from the facility at the same time as LADEE. The science mission for the spacecraft was to determine the density, composition and variability of the lunar exosphere. In addition, it performed a first-of-a-kind demonstration of laser-based communications from deep space that exhibited a record downlink rate of 622 megabits per second from the moon. In order to perform the lunar dust surveys, the spacecraft was placed in a retrograde equatorial orbit with periapsis between 20 and 60 kilometers. The mission was granted an extension in which final science surveys were performed at altitudes as low as 2 kilometers over the moon's surface. The cadence for spacecraft operations was demanding: the moon's highly inhomogeneous gravity field distorted the orbit, the regular maneuvers were subject to strict payload-induced pointing requirements, and there were periodic attitude changes to keep the spacecraft thermally safe. This led to a need for high reliability in the operation of the spacecraft while obeying strict budget and schedule guidelines.

Standards↗