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At least 109 records · Page 6

Microbiomes of commercially-available pine nuts and sesame seeds

Metagenomic analysis of food is becoming more routine and can provide important information pertaining to the shelf life potential and the safety of these products. However, less information is available on the microbiomes associated with low water activity foods. Pine nuts and sesame seeds, and food products which contain these ingredients, have been associated with recalls due to contamination with bacterial foodborne pathogens. The objective of this study was to identify the microbial community of pine nuts and sesame seeds using targeted 16S rRNA sequencing technology. Ten different brands of each seed type were assessed, and core microbiomes were determined. A total of 21 and 16 unique taxa with proportional abundances >1% in at least one brand were identified in the pine nuts and sesame seeds, respectively. Members of the core pine nut microbiome included the genera Alishewanella, Aminivibrio, Mycoplasma, Streptococcus, and unassigned OTUs in the families of Desulfobacteraceae and Xanthomonadaceae. For sesame seeds, the core microbiome included Aminivibrio, Chryseolina, Okibacterium, and unassigned OTUs in the family Flavobacteriaceae. The microbiomes of these seeds revealed that these products are dominated by environmental bacterial genera commonly isolated from soil, water, and plants; bacterial genera containing species known as commensal organisms were also identified. Understanding these microbiomes can aid in the risk assessment of these products by identifying food spoilage potential and community members which may co-enrich with foodborne bacterial pathogens.

59 BASIC BIOLOGICAL SCIENCES↗

Microbiological surveillance of food handling at NASA-MSFC

A microbiological surveillance program of cafeterias and snack bars was conducted to supplement the inspections by NASA Medical Center personnel and to gather information for cafeteria management to pinpoint areas of possible contamination. The work conducted under the program from its inception in January, 1972, to its termination on September 15, 1972 is summarized. Ten food handling facilities were included in the surveillance at NASA-MSFC.

Beyerle, F. J.↗

Semiselective Optoelectronic Sensors for Monitoring Microbes

Sensor systems are under development for use in real-time detection and quantitation of microbes in water without need for sampling. These systems include arrays of optical sensors; miniature, portable electronic data-acquisition circuits; and optoelectronic interfaces between the sensor arrays and data-acquisition circuits. These systems are intended for original use in long-term, inline monitoring of waterborne micro-organisms in water-reclamation systems aboard future spacecraft. They could also be adapted to similar terrestrial uses with respect to municipal water supplies, stored drinking water, and swimming water; for detecting low-level biological contamination in biotechnological, semiconductor, and pharmaceutical process streams; and in verifying the safety of foods and beverages. In addition, they could be adapted to monitoring of airborne microbes and of surfaces (e.g., to detect and/or quantitate biofilms). The designs of the sensors in these systems are based partly on those of sensors developed previously for monitoring airborne biological materials. The designs exploit molecular- recognition and fluorescence-spectroscopy techniques, such that in the presence of micro-organisms of interest, fluorescence signals change and the changes can be measured. These systems are characterized as semiselective because they respond to classes of micro-organisms and can be used to discriminate among the classes. This semiselectivity is a major aspect of the design: It is important to distinguish between (1) the principle of detection and quantitation of classes of micro-organisms by use of these sensors and (2) the principle of detection and quantitation of individual microbiological species by means of prior immuno-diagnostic and/or molecular-biology techniques. Detection of classes (in contradistinction to species) is particularly valuable when the exact nature of a contaminant is unknown.

Tabacco, Mary Beth↗

Progress in regenerative life support systems for a lunar laboratory.

Research and development work for application of Environmental and Thermal Control/Life Support System (ETC/LSS) on a lunar base mission is reviewed, covering lunar mission requirements and constraints, a Lunar Base ETC/LSS reliability assessment, food regeneration, the water and waste system, the atmosphere regeneration subsystem, and atmosphere contaminant control. The establishment of detailed system design criteria for the Lunar Surface Base LSS is considered to be premature at this phase of the project. Some recommendations are given instead for guidance in further R & D efforts.

Pecoraro, J. N.↗

Technology Development to Support Human Health and Performance in Exploration Beyond Low Earth Orbit

In the course of defining the level of risks and mitigating the risks for exploration missions beyond low Earth orbit, NASA s Human Research Program (HRP) has identified the need for technology development in several areas. Long duration missions increase the risk of serious medical conditions due to limited options for return to Earth; no resupply; highly limited mass, power, volume; and communication delays. New space flight compatible medical capabilities required include: diagnostic imaging, oxygen concentrator, ventilator, laboratory analysis (saliva, blood, urine), kidney stone diagnosis & treatment, IV solution preparation and delivery. Maintenance of behavioral health in such an isolated, confined and extreme environment requires new sensory stimulation (e.g., virtual reality) technology. Unobtrusive monitoring of behavioral health and treatment methods are also required. Prolonged exposure to weightlessness deconditions bone, muscle, and the cardiovascular system. Novel exercise equipment or artificial gravity are necessary to prevent deconditioning. Monitoring of the degree of deconditioning is required to ensure that countermeasures are effective. New technologies are required in all the habitable volumes (e.g., suit, capsule, habitat, exploration vehicle, lander) to provide an adequate food system, and to meet human environmental standards for air, water, and surface contamination. Communication delays require the crew to be more autonomous. Onboard decision support tools that assist crew with real-time detection and diagnosis of vehicle and habitat operational anomalies will enable greater autonomy. Multi-use shield systems are required to provide shielding from solar particle events. The HRP is pursuing the development of these technologies in laboratories, flight analog environments and the ISS so that the human health and performance risks will be acceptable with the available resources.

Kundrot, C.E.↗

Space Crop Production

As astronauts venture farther from Earth, and for longer periods, food will become increasingly critical. Crop production can supplement a packaged diet to provide additional nutrients and variety for astronauts. Testing with the Veggie and Advanced Plant Habitat chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth and nutritional content, and the importance of plants to astronauts living and working away from our home planet. The space environment presents unique challenges to crop production, and technology and knowledge gaps have been identified. Key gaps include how to: - Identify and demonstrate effective options to provide both water and oxygen to the root zone in microgravity - Understand the deep space radiation impacts on seeds and plants - Investigate the relationship between microbiomes and food safety - Store and handle seeds to ensure they are viable, free of contaminants and long-lived - Identify / develop potential crops suitable for the space environment - Understand automation and human factors - Determine scalability for different concepts and architectures Filling these gaps will help enable future human exploration and move us toward Earth-independence.

Space Crop Production↗

Assessing Effects of Climate Change on Legacy Waste at the Enewetak Atoll

The Republic of the Marshall Islands (RMI) is in the central Pacific Ocean ~4,500 km west of Hawaii. The Enewetak Atoll, located in the northwest part of the RMI, was the site for 43 nuclear weapon tests between 1948 and 1958. Fallout and deposition from the tests contaminated the island surfaces, lagoon waters and sediment, and nearby ocean waters at the atoll. In the 1970s, a cleanup effort collected radioactive waste and placed it in the Cactus Crater on Runit Island (also called the Runit Dome). In December 2021, Congress directed the U.S. Department of Energy to study the impacts of climate change on the Runit Dome nuclear waste disposal site. Pacific Northwest National Laboratory (PNNL) assembled a multidisciplinary team of climate scientists, ocean modelers, environmental scientists, and health physicists to assess the likely effects of remaining radionuclides at the Enewetak Atoll. PNNL’s approach focused on effects of tropical cyclones that were postulated to mobilize and transport contaminated lagoon sediments and result in human and biota exposure. PNNL’s study estimated (1) the radionuclide source term, (2) the effects of climate change on severe storms, (3) mobilization and transport of radionuclides, and (4) radiation dose to humans and biota. Radionuclides in the lagoon and/or ocean waters of the Enewetak Atoll were characterized by the U.S. Atomic Energy Commission (AEC) in 1972, Woods Hole Oceanographic Institution in 2015, and Lawrence Livermore National Laboratory in 2018. The RMI Nationwide Radiological Study was conducted in the early 1990s for radionuclides remaining in island soils. The 1972 AEC survey remains the most comprehensive source of radionuclide data on lagoon sediments. Climate change modeling at a regional scale in the central Pacific Ocean is limited. PNNL climate scientists simulated severe historical storms postulated to occur both in a recent climate (2015) and in the future (2090) using the Advanced Research Weather Research and Forecasting (WRF-ARW) model, employing a pseudo-global-warming technique. A postulated complete, future failure of the Runit Dome was also considered. PNNL developed a high-resolution regional ocean hydrodynamics model covering the entire RMI extended economic zone using the Finite Volume Coastal Ocean Model (FVCOM). The FVCOM model was run using global reanalysis data for current climate and WRF-ARW simulation for the future climate. PNNL also developed a radionuclide fate and transport model using the FVCOM Integrated Compartment Model (FVCOM-ICM) to simulate the current and future mobilization and transport of radionuclides sorbed to lagoon sediments and the exchange of radionuclides between the water and sediment. FVCOM-ICM-predicted radionuclide concentrations were then used to estimate radiation dose to humans and biota at all islands of the Enewetak Atoll. Under current climate conditions, annual radiation exposures for the southern islands including Enewetak (Fred) and Medren (Elmer) were below the current U.S. standards. Radiation doses were somewhat elevated starting at Runit Island northward and westward to Enjebi Island (Janet). The islands in the northwest quadrant, particularly Bokoluo (Alice) and Bokombako (Belle), remain relatively contaminated. The islands in the southwestern quadrant have low contamination. The highest contribution to radiation doses comes from consumption of locally grown foods. Two radionuclides, 90Sr and 137Cs, contributed the greatest fraction for most terrestrial foods. In current climate conditions, the storms temporarily increased radionuclide concentrations in the lagoon waters, increasing the radiation dose slightly. In future conditions, doses are expected to be smaller, primarily because of the radioactive decay of the shorter-lived radioisotopes of 90Sr and 137Cs. This could make all islands in the far northwest of the atoll – except Bokombako (Belle) and perhaps Bokoluo (Alice) – suitable for residency. For the f

Prasad, Rajiv↗

Restricted by A Historical Review of Strategies and Organization for Restricted Earth Return of Samples from NASA Planetary Missions

Return of samples from the Moon via the Apollo 11, 12 and 14 missions represented NASA's first attempts into conducting what is now known as Restricted Earth Return. When Apollo returned samples from the moon, there were few of the agencies, legal and organizational structures that exist now. A half a century later, the U.S system has seen changes in legal and organizational structures with the appearance of different agencies and legal requirements. During the Apollo era, U.S. legal and organizational structures, assessed threats to the Earth’s biosphere with a priority towards the risks of effects from extraterrestrial pathogens on humans, though the guiding document, the Baylor Protocol, also considered animal and plant models. This presentation focuses on a review of two main questions: Did the return of samples from the Moon happen at a “sweet spot” in time, where the number of requirements and conservatism was commensurate with the scientific knowns and unknowns? Could the emphasis of risk mitigation on human pathogens during the return of extraterrestrial samples be more about human solipsism, leaving us with a blind spot to sources of pathogens, such as fungi, which could lead to an impact to our biosphere, and we humans, via our food chains and agricultural pathways?

planetary protection↗

Design and performance of Skylab thermal/environmental control system

The function of the thermal/environmental control systems was to provide a comfortable thermal environment for the crew, to cool electronic components, to supply a controlled oxygen/nitrogen atmosphere, and to remove moisture, carbon dioxide, odors, and trace contaminants from the atmosphere. A separate refrigeration system was used to chill and freeze food and biomedical samples and to provide cold water for drinking. This paper describes system design and compares in-flight performance to preflight predictions. A discussion of in-flight anomalies and corrective actions is also included.

Hopson, G. D.↗

Experiments Advance Gardening at Home and in Space

Aeroponics, the process of growing plants suspended in air without soil or media, provides clean, efficient, and rapid food production. Crops can be planted and harvested year-round without interruption, and without contamination from soil, pesticides, and residue. Aeroponic systems also reduce water usage by 98 percent, fertilizer usage by 60 percent, and eliminate pesticide usage altogether. Plants grown in aeroponic systems have been shown to absorb more minerals and vitamins, making the plants healthier and potentially more nutritious. The suspended system also has other advantages. Since the growing environment can be kept clean and sterile, the chances of spreading plant diseases and infections commonly found in soil and other growing media are greatly reduced. Also, seedlings do not stretch or wilt while their roots are forming, and once the roots are developed, the plants can be easily moved into any type of growing media without the risk of transplant shock. Lastly, plants tend to grow faster in a regulated aeroponic environment, and the subsequent ease of transplant to a natural medium means a higher annual crop yield. For example, tomatoes are traditionally started in pots and transplanted to the ground at least 28 days later; growers using an aeroponic system can transplant them just 10 days after starting the plants in the growing chamber. This accelerated cycle produces six tomato crops per year, rather than the traditional one to two crop cycles. These benefits, along with the great reduction in weight by eliminating soil and much of the water required for plant growth, illustrate why this technique has found such enthusiastic support from NASA. Successful long-term missions into deep space will require crews to grow some of their own food during flight. Aeroponic crops are also a potential source of fresh oxygen and clean drinking water, and every ounce of food produced and water conserved aboard a spacecraft reduces payload weight, decreasing launch costs and freeing room for other cargo.

Source record↗

Developmental exposure to corn grown on Lake Erie dredged material: a preliminary analysis

While corn is considered to be a healthy food option, common agricultural practices, such as the application of soil amendments, might be introducing contaminants of concern (COC) into corn plants. The use of dredged material, which contain contaminants such as heavy metals, polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs), as a soil amendment is increasing. Contaminants from these amendments can accumulate in corn kernels harvested from plants grown on these sediments and potentially biomagnify in organisms that consume them. The extent to which secondary exposure to such contaminants in corn affect the mammalian central nervous system has been virtually unexplored. In this preliminary study, we examine the effects of exposure to corn grown in dredge amended soil or a commercially available feed corn on behavior and hippocampal volume in male and female rats. Perinatal exposure to dredge-amended corn altered behavior in the open-field and object recognition tasks in adulthood. Additionally, dredge-amended corn led to a reduction in hippocampal volume in male but not female adult rats. These results suggest the need for future studies examining how dredge-amended crops and/or commercially available feed corn may be exposing animals to COC that can alter neurodevelopment in a sex-specific manner. This future work will provide insight into the potential long-term consequences of soil amendment practices on the brain and behavior.

59 BASIC BIOLOGICAL SCIENCES↗

Cyrano "Nose" The Smell of Success

Cyrano Sciences, Inc. has commercialized a simple, accurate, non- invasive tool that enables "machines to smell". The Cyranose 320 is used for quality control purposes in the food and chemical industries. Using a sensor array and onboard pattern recognition algorithms, the lightweight, portable device works by exposing an array of polymer composite sensors to the chemical components in a vapor. When the sensors come in contact with the vapor, the polymer expands like a sponge, changing the resistance of the composites. The change in resistance is measured, and from that measurement, the presence of a pre-trained substance is determined with a quick and accurate diagnosis. This real-time, portable device enables food companies to spot test raw materials for batch-to-batch consistency, spoilage, or contamination. The Cyranose 320 is also used by chemical and petrochemical companies for quick assessment of the chemical status associated with various industrial processes. Profiling a chemical environment in a hazardous materials situation allows emergency crews to accurately select fire retardants, containment strategies, and protective gear. Future applications for the Cyranose 320 are fast growing and other uses of this technology are on the horizon.

Source record↗

Electrochemical production of ozone and hydrogen peroxide

Methods of using ozone have been developed which sterilize instruments and medical wastes, oxidize organics found in wastewater, clean laundry, break down contaminants in soil into a form more readily digested by microbes, kill microorganisms present in food products, and destroy toxins present in food products. The preferred methods for killing microorganisms and destroying toxins use pressurized, humidified, and concentrated ozone produced by an electrochemical cell.

Murphy, Oliver J.↗

Method of sterilization using ozone

Methods of using ozone have been developed which sterilize instruments and medical wastes, oxidize, organics found in wastewater, clean laundry, break down contaminants in soil into a form more readily digested by microbes, kill microorganisms present in food products, and destroy toxins present in food products. The preferred methods for killing microorganism and destroying toxins use pressurized, humidified, and concentrated ozone produced by an electrochemical cell.

Murphy, Oliver J.↗

Human Factors and Habitability Challenges for Mars Missions

As NASA is planning to send humans deeper into space than ever before, adequate crew health and performance will be critical for mission success. Within the NASA Human Research Program (HRP), the Space Human Factors and Habitability (SHFH) team is responsible for characterizing the risks associated with human capabilities and limitations with respect to long-duration spaceflight, and for providing mitigations (e.g., guidelines, technologies, and tools) to promote safe, reliable and productive missions. SHFH research includes three domains: Advanced Environmental Health (AEH), Advanced Food Technology (AFT), and Space Human Factors Engineering (SHFE). The AEH portfolio focuses on understanding the risk of microbial contamination of the spacecraft and on the development of standards for exposure to potential toxins such as chemicals, bacteria, fungus, and lunar/Martian dust. The two risks that the environmental health project focuses on are adverse health effects due to changes in host-microbe interactions, and risks associated with exposure to dust in planetary surface habitats. This portfolio also proposes countermeasures to these risks by making recommendations that relate to requirements for environmental quality, foods, and crew health on spacecraft and space missions. The AFT portfolio focuses on reducing the mass, volume, and waste of the entire integrated food system to be used in exploration missions, and investigating processing methods to extend the shelf life of food items up to five years, while assuring that exploration crews will have nutritious and palatable foods. The portfolio also delivers improvements in both the food itself and the technologies for storing and preparing it. SHFE sponsors research to establish human factors and habitability standards and guidelines in five risk areas, and provides improved design concepts for advanced crew interfaces and habitability systems. These risk areas include: Incompatible vehicle/habitat design, inadequate human-computer interaction, inadequate critical task design, inadequate human-automation/robotic interaction, and performance errors due to training deficiencies. To address the identified research gaps within each risk, SHFH's research plan includes studies in the laboratory, in analogs, and on International Space Station (ISS). In addition to establishing and maintaining the risk-based research portfolio, SHFH is also implementing a qualitative approach to determine how we at NASA evaluate human performance. Via interviews with experts, such as trainers, flight controllers, and flight surgeons, we are collecting the metrics by which they assess human performance, evidence of performance issues, and potential or actual consequences. The Human Performance Data Project will determine what human performance data have been collected in the past at NASA, and what data should be collected in the future in order to complete our knowledgebase and reduce risks related to human factors and habitability.

Whitmore, Mihriban↗

Novel Remediation of Mercury-Contaminated Sediments Using Manganese Oxide and Activated Carbon Amendments - 20394

Mercury contamination in sediments poses a challenging remediation problem because of its ability to biogeochemically cycle and biomagnify in the food chain as methylmercury from low resident sediment and porewater concentrations. One mitigation approach is to lower the overall rate of net methylmercury production by creating conditions unfavorable for sulfate and iron reduction or methanogenesis, the primary pathways for methylation of inorganic mercury. Manganese oxides and other redox-active amendments are a promising and novel in situ amendment technology aimed at reducing net methylation of mercury though control of redox conditions in mercury-contaminated sediment-water systems. This paper reviews the thermodynamic and kinetic framework for this remediation technology, presents experimental and spectroscopic data illustrating the approach using solid manganese oxides, and discusses potential advantages, disadvantages, and applications. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Research Animal Holding Facility Prevents Space Lab Contamination

Healthy environment for both rodents and human researchers maintained. Research animal holding facility (RAHF) and rodent cage prevent solid particles (feces, food bits, hair), micro-organisms, ammonia, and odors from escaping into outside environment during spaceflight. Rodent cage contains compartments for two animals. Provides each drinking-water dispenser, feeding alcove, and activity-monitoring port. Feeding and waste trays removable.

Savage, P. D., Jr.↗

Regenerative life support technology challenges for the Space Exploration Initiative

Regenerative life support systems have been identified as one of the critical enabling technologies for future human exploration of space. This discipline encompasses processes and subsystems which regenerate the air, water, solid waste, and food streams typical of human habitation so as to minimize the mass and volume of stored consumables which must accompany the humans on a mission. A number of key technology challenges within this broad discipline are described, ranging from the development of new physical, chemical, and biological processes for regenerating the air, water, solid waste, and food streams to the development of improved techniques for monitoring and controlling microbial and trace constituent contamination. A continuing challenge overarching the development of these new technologies is the need to minimize the mass, volume, and electrical power consumption of the flight hardware. More important for long duration exploration missions, however, is the development of highly reliable, long-lived, self- sufficient systems which absolutely minimize the logistics resupply and operational maintenance requirements of the life support system and which ensure human safety through their robust, reliable operating characteristics.

Bilardo, Vincent J., Jr.↗