Results for “Food Safety” — Page 6

NASA NTRSDate not supplied

Space Crop Production

As astronauts venture farther from Earth, and for longer periods, food will become increasingly critical. Crop production can supplement a packaged diet to provide additional nutrients and variety for astronauts. Testing with the Veggie and Advanced Plant Habitat chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth and nutritional content, and the importance of plants to astronauts living and working away from our home planet. The space environment presents unique challenges to crop production, and technology and knowledge gaps have been identified. Key gaps include how to: - Identify and demonstrate effective options to provide both water and oxygen to the root zone in microgravity - Understand the deep space radiation impacts on seeds and plants - Investigate the relationship between microbiomes and food safety - Store and handle seeds to ensure they are viable, free of contaminants and long-lived - Identify / develop potential crops suitable for the space environment - Understand automation and human factors - Determine scalability for different concepts and architectures Filling these gaps will help enable future human exploration and move us toward Earth-independence.

Space Crop Production↗

NASA NTRS2021

Applying a One Health Approach in Global Health and Medicine: Enhancing Involvement of Medical Schools and Global Health Centers

Multidisciplinary and multisectoral approaches such as One Health and related concepts (e.g., Planetary Health, EcoHealth) offer opportunities for synergistic expertise to address complex health threats. The connections between humans, animals, and the environment necessitate collaboration among sectors to comprehensively understand and reduce risks and consequences on health and wellbeing. One Health approaches are increasingly emphasized for national and international plans and strategies related to zoonotic diseases, food safety, antimicrobial resistance, and climate change, but to date, the possible applications in clinical practice and benefits impacting human health are largely missing.

Catherine Machalaba↗

NASA NTRSDate not supplied

Testing Specialized Fertigation Blends for Agronomic Biofortification for Spaceflight Applications

The current spaceflight diet is prepackaged and vitamins degrade over time. Plants grown in the Veggie and APH units on the ISS currently provide the astronauts with a hefty dose of vitamins and minerals, but some nutrients could be improved. Agronomic Biofortifcation of crops with fertilizers has been tested on Earth, with the successful addition of minerals and vitamin C to crops that have otherwise been deficient. We intend to test if we can replicate this in a controlled environment and expand it to nutrients that are not often present in abundance in plants. This will be done by adding fluid nutrient blends to the water supply of candidate crops for spaceflight applications. We hypothesize that fertilization of plants with nutrients that are of value to human health will have neutral to positive effect on plants, with desired nutrients present in greater abundance in treated plants than untreated plants. We intend to show this through germination percent and pace, yield of edible biomass, the growth rate, and overall development of the crop plant. For this experiment, radish microgreens were grown on mats in a controlled environment chamber in enriched CO2, with LED lighting similar to what is found in the ISS Veggie hardware. Watering was conducted once at planting with a specialized blend of ½ strength Hoagland’s hydroponic solution with additional vitamins. Preliminary results show no decrease in overall biomass production with plants grown using additional vitamin D, but additional nutrients will be tested. Future direction will be to perform nutritional analysis of plants. Test that resulting produce will be suitable for human consumption, including meeting or exceeding food safety guidelines and palatability.

Christina M Johnson↗

NASA NTRSDate not supplied

The Effects of Plasma Application on Radish Seeds with Implications for Space Crop Production

In extended space missions, the astronaut diet will consist mostly of prepackaged foods. This could result in nutritional deficiencies due to the gradual breakdown of certain vitamins. To address this deficiency, fresh produce must be grown from seed during spaceflight. Stored seeds, however, can be vulnerable to microbial contamination which could jeopardize plant health and crop food safety. To alleviate this concern, the current practice is to sanitize seeds on the ground before spaceflight to the International Space Station (ISS). Methods of seed sanitization include alcohol soaking and chlorine gas fumigation, which have harmful effects on the environment and human health. Plasma application is a new sanitization approach that avoids these negative side effects while potentially elevating germination rate and improving growth rate; yet plasma application requires specific exposure time, power, and pressure to achieve these benefits. In the present study, Raphanus sativus ‘Cherry Belle’ radish seeds were exposed to either low pressure (Diener) plasma or atmospheric pressure plasma for varying increments of time (30s-1200s). Data collected includes immediate germination rate (viability) and microbial log reduction. Additionally, seeds were stored for later germination rate testing. Microbial assay controls have highlighted variability in initial microbial load between individual seeds, and further work is being performed to determine the standard microbial load of an untreated seed. In some samples, a negative log reduction was observed after plasma treatment encouraging further study to determine if the plasma is perforating the seed coat and releasing endophytic microbes. Treatment with Diener plasma shows promising microbial log reduction, but lower viability. In contrast, treatment with atmospheric pressure plasma offers high viability, but poor microbial log reduction. Further study will aim at determining whether plasma treatment is effective at not only sanitization, but sterilization. This research was funded by a NASA grant at the Kennedy Space Center.

Plasma↗

NASA NTRSDate not supplied

Microbial Factories for Solar System Exploration

As humans venture deeper into space for longer duration missions, resupply missions become far more difficult and costly. To address this, alternative methods of in situ biomanufacturing can be developed to provide a reliable source of required nutrients. BioNutrients is an ongoing project including three separate flight experiments that aims to provide on-demand nutrient production through 5-year shelf stable microbial factories for astronauts on long-duration missions. The BioNutrients-3 (BN-3) flight experiment aims to demonstrate on-orbit food safety, user-friendly production pack design, and multi-compound production capability. This current study seeks to support the multiple compound production element of BN-3 by investigating the production of multiple compounds via multiple pathways in one organism. An experiment has been designed to modify an existing strain of Saccharomyces boulardii designed by the BioNutrients team to efficiently produce β-carotene to also efficiently produce a second nutrient, riboflavin. This experiment entails the random shuffling and recombination of gene elements including promoters and terminators of various strengths to identify optimal combinations for the upregulation of the riboflavin production pathway. Implementation of this experiment would provide insight into the efficiency of the multiple pathways production method and pave the way for additional fine tuning of the riboflavin production pathway and additional nutrient production pathways in S. boulardii.

Biomanufacturing↗

NASA NTRS2021

What are Needed Innovations for Space Crop Production That May Also Benefit Vertical Farming?

I am speaking about the space crop production gaps and challenges we face and how we need innovations in the area of plant health monitoring, food safety contamination monitoring, CEA IPM, understanding of and manipulation of the microbiome/ecosystem, sustainability needs, novel sensors needed, automation and robotics, and also new crops that could be developed and ways that our needs parallel those of the vertical farming industry. (Note: This presentation is in video mp4 format is only viewable by download)

Gioia Massa↗

NASA NTRSDate not supplied

Testing Specialized Fertigation Blends for Agronomic Biofortification for Spaceflight Applications

The current spaceflight diet is prepackaged and vitamins degrade over time. Plants grown in the Veggie and APH units on the ISS currently provide the astronauts with a hefty dose of vitamins and minerals, but some nutrients could be improved. Agronomic Biofortifcation of crops with fertilizers has been tested on Earth, with the successful addition of minerals and vitamin C to crops that have otherwise been deficient. We intend to test if we can replicate this in a controlled environment and expand it to nutrients that are not often present in abundance in plants. This will be done by adding fluid nutrient blends to the water supply of candidate crops for spaceflight applications. We hypothesize that fertilization of plants with nutrients that are of value to human health will have neutral to positive effect on plants, with desired nutrients present in greater abundance in treated plants than untreated plants. We intend to show this through germination percent and pace, yield of edible biomass, the growth rate, and overall development of the crop plant. For this experiment, radish microgreens were grown on mats in a controlled environment chamber in enriched CO2, with LED lighting similar to what is found in the ISS Veggie hardware. Watering was conducted once at planting with a specialized blend of ½ strength Hoagland’s hydroponic solution with additional vitamins. Preliminary results show no decrease in overall biomass production with plants grown using additional vitamin D, but additional nutrients will be tested. Future direction will be to perform nutritional analysis of plants. Test that resulting produce will be suitable for human consumption, including meeting or exceeding food safety guidelines and palatability.

microgreens↗

NASA NTRSDate not supplied

The Effects of Plasma Application on Radish Seeds with Implications for Space Crop Production

In extended space missions, the astronaut diet will consist mostly of prepackaged foods. This could result in nutritional deficiencies due to the gradual breakdown of certain vitamins. To address this deficiency, fresh produce must be grown from seed during spaceflight. Stored seeds, however, can be vulnerable to microbial contamination which could jeopardize plant health and crop food safety. To alleviate this concern, the current practice is to sanitize seeds on the ground before spaceflight to the International Space Station (ISS). Methods of seed sanitization include alcohol soaking and chlorine gas fumigation, which have harmful effects on the environment and human health. Plasma application is a new sanitization approach that avoids these negative side effects while potentially elevating germination rate and improving growth rate; yet plasma application requires specific exposure time, power, and pressure to achieve these benefits. In the present study, Raphanus sativus ‘Cherry Belle’ radish seeds were exposed to either low pressure (Diener) plasma or atmospheric pressure plasma for varying increments of time (30s-1200s). Data collected includes immediate germination rate (viability) and microbial log reduction. Additionally, seeds were stored for later germination rate testing. Microbial assay controls have highlighted variability in initial microbial load between individual seeds, and further work is being performed to determine the standard microbial load of an untreated seed. In some samples, a negative log reduction was observed after plasma treatment encouraging further study to determine if the plasma is perforating the seed coat and releasing endophytic microbes. Treatment with Diener plasma shows promising microbial log reduction, but lower viability. In contrast, treatment with atmospheric pressure plasma offers high viability, but poor microbial log reduction. Further study will aim at determining whether plasma treatment is effective at not only sanitization, but sterilization. This research was funded by a NASA grant at the Kennedy Space Center.

Space Crop Production↗

NASA NTRSDate not supplied

The Microbiology of Microgreens Grown in Controlled Environment Chambers under ISS Conditions

Microgreens have been identified as a new type of pick-and-eat salad crop that can be utilized in space crop production systems. The majority of traditionally grown leafy green crops can be grown as microgreens, in addition to crops such as legumes, sunflower, buckwheat, most herbs, and corn, presenting hundreds of microgreen crop options. Notably, microgreens are nutrient dense, high in beneficial compounds like antioxidants, Vitamins C and K, and exhibit a variety of desirable flavors and textures.The short growth cycles (7-14 days), low water requirements and volume optimization potential make them a viable option for sustainable production of nutritious and flavorful crops in space.The crop production team at Kennedy Space Center is investigating the food safety aspects of microgreens grown under spaceflight relevant conditions for crew consumption. Microbiological analysis and screening for potential foodborne pathogens was performed on over 20 varieties of microgreens that have demonstrated positive horticultural attributes.Additionally, a comparison of microgreens grown hydroponically under ISS environmental conditions and similar varieties from local markets was completed to collect baseline data on the microbial load on microgreens. In aneffort to improve microgreen quality, strategies to reduce the microbial load were tested, including bulk seed sanitization, harvest age, exposure to high blue light, and post-harvest chemical disinfection. The efficacy of a citric acid-based produce wash currently used for ISS grown produce and 1% H2O2were investigated at different exposure times for reduction in bacterial and fungal counts on a variety of microgreens. Limited log reduction was achieved depending on exposure time.Our testing also demonstrated that seed sanitization impacted microbial load on microgreens and systems.

Mary E. Hummerick↗

NASA NTRS2023

Legume Crop Testing for Space

Long-duration missions beyond low-Earth orbit will encounter challenges in maintaining adequate nutrition and crew acceptability in the food system. In situ production of fresh produce can supplement nutrient deficiencies in the prepackaged diet. Currently, there are a relatively small number of crops that can be reliably grown for space crop production efforts. Recent challenges with Veggie plant growth technical demonstrations, such as interveinal chlorosis and necrosis of Tokyo Bekana Chinese cabbage when grown under elevated CO 2 (~3000 ppm) and narrow-band LED lighting, have highlighted the necessity to conduct rigorous ISS-relevant crop screening on the ground. Additionally, crops should be selected to address specific nutritional deficits, as identified by NASA’s Human Research Program, with an emphasis on having a diversity of crops to meet nutritional requirements and crew acceptability. To achieve this, the concept of Crop Readiness Level (CRL) has been developed to gauge readiness of crops for spaceflight applications. CRL determination includes assessing environmental compatibility, food safety considerations, relevant nutritional analysis, and sensory analysis. Recent testing at Kennedy Space Center has focused on advancing the CRL of a variety of legumes. Twenty-four varieties of peas ( Pisum sativum ) and beans ( Phaseolus vulgaris ) were grown under 300 μmol m -2 s -1 PPFD from LED lights, 3000 ppm CO2, and 23 °C to simulate an ISS environment. Crops were harvested and size and yield were assessed. Then, baseline nutritional analysis (Vitamins B1, C, K; elemental analysis; proximate analysis) and sensory evaluation were performed on eight down-selected varieties. These baseline tests will help in selecting candidate crops for future missions and assessing crop production hardware and changes in environmental conditions on future crop performance and nutritional quality.

LaShelle E Spencer↗

NASA NTRS2023

3D Printed Substrate

In preparation for long-term, manned, deep space missions, NASA requires a sustainable system for crop and food production. This system has a variety of benefits, including a fresh food supply, improvements in air quality, a lower need to resupply and psychological benefits for the gardeners. Specifically, NASA is attempting to improve and iterate their Vegetable Production System (VEGGIE), a plant growth unit currently aboard the International Space Station (ISS). The VEGGIE unit is highly dependent on a variety of factors, including passive water delivery, growth lights, rooting pillows and cabin conditions. While previous teams have improved and redesigned water reservoirs, the primary objective of the project was to create a new substrate unit to replace the current rooting pillow design. The current design consists of an electrostatic bag filled with arcillite and a slow-release fertilizer pellet. The prototype design retains the fertilizer pellet for nutrient consistency, however the outer bag and arcillite fillings have been replaced with a 3D printed lattice block. This lattice block has several key points that allow it to function in similar ways to the current design: 1. The lattice planes are porous, with each pore offset such that the overall porosity is the same as the arcillite filling. 2. The block demonstrates a wicking nature and is able to pull water upwards towards the roots. This minimizes time needed to integrate with the existing water reservoir. 3. The filament used is highly flexible and is able to pull apart to accommodate root growth. 4. The lattice planes are connected by microfibers left behind from the printing process. These fibers provide support for the growing roots and keep the lattice planes properly aligned. The substrate block design seeks to reduce the payload costs by being entirely 3D printed. While filament would still need to be provided to the ISS, it is far less expensive than shipping arcillite due to the significantly lower weight. The final design has iterated the lattice plane concept and utilizes vertically placed planes with pores running parallel to the water reservoir. This design has reliably shown water uptake and retention and has been successful in growing multiple romaine lettuce plants. Future work should include further growth testing using multiple plant species, compost and reusability testing, food safety testing and microgravity growth testing.

Affan Bhutta↗

NASA NTRS2025

The Microbiome of A Tomato Crop Grown Under Different Lighting Regimes on the International Space Station

The VEG-05 experiment presented here investigated the effect of red-rich and blue-rich light recipes in Veggie on the microbiome of the Veggie facility and the plant tissues of a dwarf tomato variety, Solanum lycopersicum cv. Red Robin. For food safety, the plants were screened using culture-based methods for potential human pathogens that may cause infection by consumption of the fruit. The microbiome was investigated using bacterial 16S and fungal ITS sequencing methods to enumerate and identify bacterial and fungal communities on tomato fruit, roots, leaves, rooting substrate, and Veggie facility surfaces grown under blue-rich or red-rich lighting. Comparisons of microbial communities were made between lighting treatments, as well as for flight and ground controls. This analysis determined the core microbiome and microbiological composition for tomato plants grown under a blue-rich or red-rich lighting treatment and microgravity conditions. Culture-based pathogen screening, corroborated by 16S and ITS sequencing, yielded negative results. Bacterial and fungal counts were lower for ground controls than in-flight samples. However, there were no differences in microbial counts between lighting treatments. Regardless of lighting treatment, plant components shared a core microbiome, although some differences were observed in genera between lighting treatments.

Veggie↗

NASA NTRSDate not supplied

Space Crop Production Gaps and Challenges

As astronauts venture farther from Earth, and stay for longer periods, the space food system will increase in importance. Crop production can supplement a pre-packaged space diet to provide nutrition and dietary variety for space crews. In future missions, bioregenerative approaches may be used to generate a larger percentage of the diet, as well as help to reduce life support system burdens and resupply from Earth. Plants may also provide behavioral health benefits to crew members living in the isolated, confined environment of a space habitat. A number of unique challenges exist for growth of plants in microgravity and on other reduced gravity surfaces like the moon and Mars. Testing with the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, acceptability, and the importance of plants to astronauts living and working away from Earth. We are also gaining a better understanding of food safety concerns and the behavior of space plant microbiomes and plant pathogens, but major gaps in knowledge remain. As we move from research towards operational space crop production to enable exploration, there are numerous gaps in technology, knowledge, and practice related to space crop growth that must be addressed.

Gioia Massa↗

DOE OSTI2024

Frozen Freedom: Unleashing Grocery Store Demand Flexibility: Preprint

Grocery stores consumed approximately 3% of total electricity used by commercial buildings in the U.S. in 2018 (EIA 2018), representing a unique end-use load profile characterized by the critical use of refrigerated display cases. Exploring demand response (DR) scenarios in grocery stores presents an opportunity to enhance the efficiency and sustainability of surrounding communities. In addition, recent studies demonstrate that implementing control algorithms considering demand flexibility strategies can lead to load and peak reductions in standalone refrigerated display cases. Because small business grocery stores operate on thin margins, the energy bill cost savings DR might provide could make a positive difference toward continued operations. Still, uncertainty remains about the extent of demand flexibility potential controls could provide when coupling refrigeration with whole building operation. To enhance economic viability and grid stability, it is essential to quantify the load flexibility capability of grocery stores. Advanced controls can optimize energy consumption by responding to load shedding, shifting, and DR events, as well as daily Time-of-Use (TOU) rates without compromising food safety. Using both quantitative data and interviews with community-based organizations, we developed a full-size store model and two small store models with controlled refrigerated cases, HVAC, and lighting systems based on actual grocery store properties. Through simulations, we have assessed load flexibility strategies with varied DR events. The results highlight potential for energy and peak reduction with advanced or basic controls. However, interviews and data indicate that more support is needed to make DR strategies consistently accessible to small grocery stores.

demand flexibility↗

DOE OSTI2025

Fate of Listeria monocytogenes Serotypes on Frozen Mixed Vegetables During Consumer‐Simulated Thawing and Storage

ABSTRACT Recent outbreaks and recalls associated with frozen vegetables in the United States and Europe have been linked to Listeria monocytogenes . This study aims to understand the extent to which frozen vegetables support the growth of L. monocytogenes once thawed and held at different temperatures. Six L. monocytogenes strains, two of each from serotypes 1/2a, 1/2b, and 4b, were individually inoculated onto frozen vegetables and stored at −18°C for 7 days. After 7 days, the vegetables were thawed and stored at 5°C or 10°C for up to 14 days or at 25°C for up to 7 days. L. monocytogenes was enumerated from the thawed vegetables throughout the storage period. Population data were fitted to the primary Baranyi model to estimate growth rates and lag phase durations; the secondary Ratkowsky square root model was used to model the relationship of the growth rates with storage temperature. Five of the L. monocytogenes strains survived and grew on the thawed vegetables (population increases of > 1 log CFU/g) stored at 5°C, and all six of the strains proliferated at 10°C and 25°C (population increases of > 3 log CFU/g after 14 days and > 4 log CFU/g after 7 days, respectively). A secondary model was successfully generated based on the growth rates of the six L. monocytogenes strains on the thawed vegetables ( r 2 = 0.8888, RMSE = 0.2057). Results from this study fill a data gap associated with L. monocytogenes survival on thawed vegetables and can be used to determine safe handling and storage practices for these products to protect public health.

Salazar, Joelle K. [Division of Food Processing Sc↗

DOE OSTI2025

Three‐trophic level food webs support the safety of a biocontrol agent 3 years after release

Biological control (biocontrol) is a powerful tool for managing invasive alien species and assisting the restoration of native ecosystems. Rigorous post‐release monitoring of biocontrol agents is critical to evaluate the success of biocontrol programs; however, this is still rarely implemented. Here, we combined the use of species interaction networks with a Before‐After Control‐Impact design to evaluate the target and non‐target, direct and indirect effects of the Australian gall wasp Trichilogaster acaciaelongifoliae , released to control the invasive plant Acacia longifolia in Portugal. We compared the structure of plant‐galling insect‐parasitoid food webs before and 3 years after the release of the biocontrol agent. Exhaustive sampling did not detect any non‐target effects, either direct (on non‐target plants) or indirect (on other galling insects via shared plants). Additionally, no significant changes were detected in network structure that could be related to the establishment of the biocontrol agent. This study shows that monitoring biocontrol at the community level is possible and that, when carefully planned, biocontrol poses minimal risk of non‐target effects.

López‐Núñez, Francisco A. [Centre for Functional E↗

NASA NTRS2015

Veggie ISS Validation Test Results and Produce Consumption

The Veggie vegetable production system flew to the International Space Station (ISS) in the spring of 2014. The first set of plants, Outredgeous red romaine lettuce, was grown, harvested, frozen, and returned to Earth in October. Ground control and flight plant tissue was sub-sectioned for microbial analysis, anthocyanin antioxidant phenolic analysis, and elemental analysis. Microbial analysis was also performed on samples swabbed on orbit from plants, Veggie bellows, and plant pillow surfaces, on water samples, and on samples of roots, media, and wick material from two returned plant pillows. Microbial levels of plants were comparable to ground controls, with some differences in community composition. The range in aerobic bacterial plate counts between individual plants was much greater in the ground controls than in flight plants. No pathogens were found. Anthocyanin concentrations were the same between ground and flight plants, while antioxidant and phenolic levels were slightly higher in flight plants. Elements varied, but key target elements for astronaut nutrition were similar between ground and flight plants. Aerobic plate counts of the flight plant pillow components were significantly higher than ground controls. Surface swab samples showed low microbial counts, with most below detection limits. Flight plant microbial levels were less than bacterial guidelines set for non-thermostabalized food and near or below those for fungi. These guidelines are not for fresh produce but are the closest approximate standards. Forward work includes the development of standards for space-grown produce. A produce consumption strategy for Veggie on ISS includes pre-flight assessments of all crops to down select candidates, wiping flight-grown plants with sanitizing food wipes, and regular Veggie hardware cleaning and microbial monitoring. Produce then could be consumed by astronauts, however some plant material would be reserved and returned for analysis. Implementation of this plan is a step toward developing pick-and-eat food production to supplement the packaged diet on ISS and for future exploration missions where plants could make up a larger portion of the diet. Supported by NASA Space Biology Program.

Food Safety↗

DOE OSTI2021

Detection of Foreign Materials on Broiler Breast Meat Using a Fusion of Visible Near-Infrared and Short-Wave Infrared Hyperspectral Imaging

Foreign material (FM) found on a poultry product lowers the quality and safety of the product. We developed a fusion method combining two hyperspectral imaging (HSI) modalities in the visible-near infrared (VNIR) range of 400–1000 nm and the short-wave infrared (SWIR) range of 1000–2500 nm for the detection of FMs on the surface of fresh raw broiler breast fillets. Thirty different types of FMs that could be commonly found in poultry processing plants were used as samples and prepared in two different sizes (5 × 5 mm 2 and 2 × 2 mm 2 ). The accuracies of the developed Fusion model for detecting 2 × 2 mm 2 pieces of polymer, wood, and metal were 95%, 95%, and 81%, respectively, while the detection accuracies of the Fusion model for detecting 5 × 5 mm 2 pieces of polymer, wood, and metal were all 100%. The performance of the Fusion model was higher than the VNIR- and SWIR-based detection models by 18% and 5%, respectively, when F1 scores were compared, and by 38% and 5%, when average detection rates were compared. The study results suggested that the fusion of two HSI modalities could detect FMs more effectively than a single HSI modality.

36 MATERIALS SCIENCE↗