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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 109 records · Page 6
All sourcesNASA NTRSDOE OSTI
NASA NTRS2016

Innovative Approach for Developing Spacecraft Interior Acoustic Requirement Allocation

The Orion Multi-Purpose Crew Vehicle (MPCV) is an American spacecraft for carrying four astronauts during deep space missions. This paper describes an innovative application of Power Injection Method (PIM) for allocating Orion cabin continuous noise Sound Pressure Level (SPL) limits to the sound power level (PWL) limits of major noise sources in the Environmental Control and Life Support System (ECLSS) during all mission phases. PIM is simulated using both Statistical Energy Analysis (SEA) and Hybrid Statistical Energy Analysis-Finite Element (SEA-FE) models of the Orion MPCV to obtain the transfer matrix from the PWL of the noise sources to the acoustic energies of the receivers, i.e., the cavities associated with the cabin habitable volume. The goal of the allocation strategy is to control the total energy of cabin habitable volume for maintaining the required SPL limits. Simulations are used to demonstrate that applying the allocated PWLs to the noise sources in the models indeed reproduces the SPL limits in the habitable volume. The effects of Noise Control Treatment (NCT) on allocated noise source PWLs are investigated. The measurement of source PWLs of involved fan and pump development units are also discussed as it is related to some case-specific details of the allocation strategy discussed here.

Chu, S. Reynold↗
NASA NTRS1990

Approach to SSME health monitoring. II - Exhaust plume emission spectroscopy at the DTF

The Diagnostics Testbed Facility (DTF) located at the Stennis Space Center (SSC) is used for obtaining extensive sets of H2O2 exhaust plume emission spectral data for the SSME critical components related elements and materials. The SSME related elements and materials are simulated by mixing appropriate amounts of compounds of their respective constituent elements in an aqueous solution which is injected into the combustion chamber of the DTFT. Five of the most critical components of the SSME which have experienced very severe wear and tear problems in the past are analyzed. These are high pressure turbopump (HPTP) turbine blades, HPTP turbine disks, HPTP bearing, main injector LOX posts, and the main combustion chamber structural shell. The alloys used in the manufacturing of these components are MAR-M 246 + Hf, Waspaloy X, AISI 440C, Haynes 188, and Inconel 718, respectively. The experimental setup and procedures at the DTF are described; stratospheric data for the five alloys are presented; and strategies for the material identification in the SSME exhaust plume are discussed.

Tejwani, Gopal D.↗
NASA NTRS1988

Global Geospace Science Programme

The Global Geospace Science (GGS) Program, an element of the international Solar Terrestrial Physics Program dedicated to the study of the global plasma dynamics of the solar-terrestrial environment, is discussed. Past research on the injection of solar wind ions into the magnetosphere and on the detection of ions in the terrestrial ring current of both solar wind and ionospheric origins is reviewed, showing its relevance for the GGS program. Research on the interplanetary magnetic field, the auroral electrojet, the outer magnetosphere, the geomagnetic tail, the ionospheric electric field and the related electron precipitation is also addressed. The results demonstrate that the solar wind and the ionsophere both contribute to the magnetospheric particle population. Unanswered questions regarding hot plasma sources, transport processes, energy storage in the magnetic field, and energization of plasmas are discussed. The relevant mission strategy, instrumentation, theory and modeling, and data collection are addressed.

Parks, George↗
NASA NTRS2004

Development of an Intelligent Videogrammetric Wind Tunnel Measurement System

A videogrammetric technique developed at NASA Langley Research Center has been used at five NASA facilities at the Langley and Ames Research Centers for deformation measurements on a number of sting mounted and semispan models. These include high-speed research and transport models tested over a wide range of aerodynamic conditions including subsonic, transonic, and supersonic regimes. The technique, based on digital photogrammetry, has been used to measure model attitude, deformation, and sting bending. In addition, the technique has been used to study model injection rate effects and to calibrate and validate methods for predicting static aeroelastic deformations of wind tunnel models. An effort is currently underway to develop an intelligent videogrammetric measurement system that will be both useful and usable in large production wind tunnels while providing accurate data in a robust and timely manner. Designed to encode a higher degree of knowledge through computer vision, the system features advanced pattern recognition techniques to improve automated location and identification of targets placed on the wind tunnel model to be used for aerodynamic measurements such as attitude and deformation. This paper will describe the development and strategy of the new intelligent system that was used in a recent test at a large transonic wind tunnel.

Graves, Sharon S.↗
NASA NTRS2003

2001: A Space Odyssey Revisited: The Feasibility of 24 Hour Commuter Flights to the Moon Using NTR Propulsion with LUNOX Afterburners

The prospects for 24 hour commuter flights to the Moon, similar to that portrayed in 2001: A Space Odyssey but on a more Spartan scale, are examined using two near term, high leverage technologies: liquid oxygen (LOX)-augmented nuclear thermal rocket (NTR) propulsion and lunar-derived oxygen (LUNOX) production. Iron-rich volcanic glass, or orange soil, discovered during the Apollo 17 mission to Taurus-Littrow, has produced a 4 percent oxygen yield in recent NASA experiments using hydrogen reduction. LUNOX development and utilization would eliminate the need to transport oxygen supplies from Earth and is expected to dramatically reduce the size, cost and complexity of space transportation systems. The LOX-augmented NTR concept (LANTR) exploits the high performance capability of the conventional liquid hydrogen (LH2)-cooled NTR and the mission leverage provided by LUNOX in a unique way. LANTR utilizes the large divergent section of its nozzle as an afterburner into which oxygen is injected and supersonically combusted with nuclear preheated hydrogen emerging from the engine's choked sonic throat, essentially scramjet propulsion in reverse. By varying the oxygen-to-hydrogen mixture ratio, the LANTR engine can operate over a wide range of thrust and specific impulse (Isp) values while the reactor core power level remains relatively constant. The thrust augmentation feature of LANTR means that big engine performance can be obtained using smaller, more affordable, easier to test NTR engines. The use of high-density LOX in place of low density LH2 also reduces hydrogen mass and tank volume resulting in smaller space vehicles. An implementation strategy and evolutionary lunar mission architecture is outlined which requires only Shuttle C or in-line Shuttle-derived launch vehicles, and utilizes conventional NTR-powered lunar transfer vehicles (LTVs), operating in an expendable mode initially, to maximize delivered surface payload on each mission. The increased payload is dedicated to installing modular LUNOX production units with the intent of supplying LUNOX to lunar landing vehicles (LLVs) and then LTVs at the earliest possible opportunity. Once LUNOX becomes available in low lunar orbit (LLO), monopropellant NTRs would be outfitted with an oxygen propellant module, feed system and afterburner nozzle for bipropellant operation. Transition to a reusable mission architecture now occurs with smaller, LANTR-powered LTVs delivering ~400% more payload on each piloted round trip mission than earlier expendable all LH2 NTR systems. As initial lunar outposts grow to eventual lunar settlements and LUNOX production capacity increases, the LANTR concept can enable a rapid commuter shuttle capable of 24 hour one way trips to and from the Moon. A vast deposit of iron-rich volcanic glass beads identified at just one candidate site located at the southeastern edge of Mare Serenitatis could supply sufficient LUNOX to support daily commuter flights to the Moon for the next 9000 years!

Borowski, Stanley↗
NASA NTRS1998

"2001: A Space Odyssey" Revisited--The Feasibility of 24 Hour Commuter Flights to the Moon Using NTR Propulsion with LUNOX Afterburners

The prospects for "24 hour" commuter flights to the Moon, similar to that portrayed in 2001: A Space Odyssey but on a more Spartan scale, are examined using two near term, "high leverage" technologies--liquid oxygen (LOX)-augmented nuclear thermal rocket (NTR) propulsion and "lunar-derived" oxygen (LUNOX) production. Ironrich volcanic glass, or "orange soil," discovered during the Apollo 17 mission to Taurus-Littrow, has produced a 4% oxygen yield in recent NASA experiments using hydrogen reduction. LUNOX development and utilization would eliminate the need to transport oxygen supplies from Earth and is expected to dramatically reduce the size, cost and complexity of space transportation systems. The LOX-augmented NTR concept (LANTR) exploits the high performance capability of the conventional liquid hydrogen (LH2)-cooled NTR and the mission leverage provided by LUNOX in a unique way, LANTR utilizes the large divergent section of its nozzle as an "afterburner" into which oxygen is injected and supersonically combusted with nuclear preheated hydrogen emerging front the engine's choked sonic throat--essentially "scramjet propulsion in reverse." By varying the oxygen-to-hydrogen mixture ratio, the LANTR engine can operate over a wide range of thrust and specific impulse (Isp) values while the reactor core power level remains relatively constant. The thrust augmentation feature of LANTR means that "big engine" performance can be obtained using smaller, more affordable, easier to test NTR engines. The use of high-density LOX in place of low-density LH2 also reduces hydrogen mass and tank volume resulting in smaller space vehicles. An implementation strategy and evolutionary lunar mission architecture is outlined which requires only Shuttle C or "in-line" Shuttle-derived launch vehicles, and utilizes conventional NTR-powered lunar transfer vehicles (LTVs), operating in an "expendable mode" initially, to maximize delivered surface payload on each mission. The increased payload is dedicated to installing "modular" LUNOX production units with the intent of supplying LUNOX to lunar landing vehicles (LLVS) and then LTVs at the earliest possible opportunity. Once LUNOX becomes available in low lunar orbit (LLO), monopropellant NTRs would be outfitted with an oxygen propellant module, feed system and afterburner nozzle for "bipropellant" operation. Transition to a "reusable" mission architecture now occurs with smaller, LANTR-powered LTVs delivering approximately 400% more payload on each piloted round trip mission than earlier expendable "all LH2" NTR systems. As initial lunar outposts grow to eventual lunar settlements and LUNOX production capacity increases, the LANTR concept can enable a rapid "commuter" shuttle capable of 24 hour "one way" trips to and from the Moon, A vast deposit of"iron-rich" volcanic glass beads identified at just one candidate site--located at the southeastern edge of Mare Serenitatis--could supply, sufficient LUNOX to support daily commuter flights to the Moon for the next 9000 years!

Borowski, Stanley K.↗
NASA NTRS2025

An Efficient GPU-Accelerated Multi-Source Global Fit Pipeline for LISA Data Analysis

The large-scale analysis task of deciphering gravitational wave signals in the LISA data stream will be difficult, requiring a large amount of computational resources and extensive development of computational methods. Its high dimensionality, multiple model types, and complicated noise profile require a global fit to all parameters and input models simultaneously. In this work, we detail our global fit algorithm, called “Erebor,” designed to accomplish this challenging task. It is capable of analysing current state-of-the-art datasets and then growing into the future as more pieces of the pipeline are completed and added. We describe our pipeline strategy, the algorithmic setup, and the results from our analysis of the LDC2A Sangria dataset, which contains Massive Black Hole Binaries, compact Galactic Binaries, and a parameterized noise spectrum whose parameters are unknown to the user. The Erebor algorithm includes three unique and very useful contributions: GPU acceleration for enhanced computational efficiency; ensemble MCMC sampling with multiple MCMC walkers per temperature for better mixing and parallelized sample creation; and special online updates to reversible-jump (or trans-dimensional) sampling distributions to ensure sampler mixing and accurate initial estimates for detectable sources in the data. We recover posterior distributions for all 15 (6) of the injected MBHBs in the LDC2A training (hidden) dataset. We catalog ∼12000 Galactic Binaries (∼8000 as high confidence detections) for both the training and hidden datasets. All of the sources and their posterior distributions are provided in publicly available catalogs.

LISA global fit↗
NASA NTRS2001

"2001: A Space Odyssey" Revisited: The Feasibility of 24 Hour Commuter Flights to the Moon Using NTR Propulsion with LUNOX Afterburners

The prospects for "24 hour" commuter flights to the Moon. similar to that portrayed in 2001: A Space Odyssey but on a more Spartan scale. are examined using two near term. "high leverage" technologies-liquid oxygen (LOX)-augmented nuclear thermal rocket (NTR) propulsion and "lunar-derived" oxygen (LUNOX) production. Iron-rich volcanic glass. or "orange soil," discovered during the Apollo 17 mission to Taurus-Littrow. has produced a 4% oxygen yield in recent NASA experiments using hydrogen reduction. LUNOX development and utilization would eliminate the need to transport oxygen supplies from Earth and is expected to dramatically reduce the size, cost and complexity of space transportation systems. The LOX-augmented NTR concept (LANTR) exploits the high performance capability of the conventional liquid hydrogen (LH2)-cooled NTR and the mission leverage provided by LUNOX in a unique way. LANTR utilizes the large divergent section of its nozzle as an "afterburner" into which oxygen is injected and supersonically combusted with nuclear preheated hydrogen emerging from the engine's choked sonic throat-essentially "scramjet propulsion in reverse." By varying the oxygen-to-hydrogen mixture ratio, the LANTR engine can operate over a wide range of thrust and specific impulse (Isp) values while the reactor core power level remains relatively constant. The thrust augmentation feature of LANTR means that "big engine" performance can be obtained using smaller. more affordable. easier to test NTR engines. The use of high-density LOX in place of low-density LH2 also reduces hydrogen mass and tank volume resulting in smaller space vehicles. An implementation strategy and evolutionary lunar mission architecture is outlined which requires only Shuttle C or "in-line" Shuttle-derived launch vehicles, and utilizes conventional NTR-powered lunar transfer vehicles (LTVs), operating in an "expendable mode" initially, to maximize delivered surface payload on each mission. The increased payload is dedicated to installing "modular" LUNOX production units with the intent of supplying LUNOX to lunar landing vehicles (LLVs) and then LTVs at the earliest possible opportunity. Once LUNOX becomes available in low lunar orbit (LLO), monopropellant NTRs would be outfitted with an oxygen propellant module, feed system and afterburner nozzle for "bipropellant" operation. Transition to a "reusable" mission architecture now occurs with smaller, LANTR-powered LTVs delivering approximately 400% more payload on each piloted round trip mission than earlier expendable "all LH2" NTR systems. As initial lunar outposts grow to eventual lunar settlements and LUNOX production capacity increases, the LANTR concept can enable a rapid "commuter" shuttle capable of 24 hour "one way" trips to and from the Moon. A vast deposit of "iron-rich" volcanic glass beads identified at just one candidate site - located at the southeastern edge of Mare Serenitatis - could supply sufficient LUNOX to support daily commuter flights to the Moon for the next 9000 years'.

Borowski, Stanley K.↗
NASA NTRS2025

Efficient GPU-Accelerated MultiSource Global Fit Pipeline for LISA Data Analysis

The large-scale analysis task of deciphering gravitational-wave signals in the LISA data stream will be difficult, requiring a large amount of computational resources and extensive development of computational methods. Its high dimensionality, multiple model types, and complicated noise profile require a global fit to all parameters and input models simultaneously. In this work, we detail our global fit algorithm, called “Erebor,” designed to accomplish this challenging task. It is capable of analyzing current state-of-the-art datasets and then growing into the future as more pieces of the pipeline are completed and added. We describe our pipeline strategy, the algorithmic setup, and the results from our analysis of the LDC2A Sangria dataset, which contains massive black hole binaries, compact galactic binaries, and a parametrized noise spectrum whose parameters are unknown to the user. The Erebor algorithm includes three unique and very useful contributions: GPU acceleration for enhanced computational efficiency; ensemble Markov Chain Monte Carlo (MCMC) sampling with multiple MCMC walkers per temperature for better mixing and parallelized sample creation; and special online updates to reversible-jump (or transdimensional) sampling distributions to ensure sampler mixing and accurate initial estimates for detectable sources in the data.We recover posterior distributions for all 15 (6) of the injected massive black hole binaries (MBHB) in the LDC2A training (hidden) dataset. We catalog ∼12000 galactic binaries (∼8000 as high confidence detections) for both the training and hidden datasets. All of the sources and their posterior distributions are provided in publicly available catalogs.

LISA↗
NASA NTRS2004

Spitzer Orbit Determination During In-orbit Checkout Phase

The Spitzer Space Telescope was injected into heliocentric orbit on August 25, 2003 to observe and study astrophysical phenomena in the infrared range of frequencies. The initial 60 days was dedicated to Spitzer's "In-Orbit Checkout (IOC)" efforts. During this time high levels of Helium venting were used to cool down the telescope. Attitude control was done using reaction wheels, which in turn were de-saturated using cold gas Nitrogen thrusting. Dense tracking data (nearly continuous) by the Deep Space network (DSN) were used to perform orbit determination and to assess any possible venting imbalance. Only Doppler data were available for navigation. This paper deals with navigation efforts during the IOC phase. It includes Dust Cover Ejection (DCE) monitoring, orbit determination strategy validation and results and assessment of non-gravitational accelerations acting on Spitzer including that due to possible imbalance in Helium venting.

helium venting↗
NASA NTRS1994

Use of promethazine to hasten adaptation to provocative motion

In an earlier study, the authors found that severely motion sick individuals could be greatly relieved of their symptoms by intramuscular injections of promethazine (50 mg) or scopolamine (.5 mg). Comparable 50-mg injections of promethazine also have been found effective in alleviating symptoms of space motion sickness. The concern has risen, however, that such drugs may delay or retard the acquisition of adaptation to stressful environments. In the current study, we controlled arousal using a mental arithmetic task and precisely equated the exposure history (number of head movements during rotation) of a placebo, control group and an experimental group who had received promethazine. No differences in total adaptation or in rates of adaptation were present between the two groups. Another experimental group also received promethazine and was allowed to make as many head movements as they could, before reaching nausea, up to 800. This group showed a greater level of adaptation than the placebo group. These results suggest a strategy for dealing with space motion sickness that is described.

Non-NASA Center↗
NASA NTRS1989

Predeployment validation of fault-tolerant systems through software-implemented fault insertion

Fault injection-based automated testing (FIAT) environment, which can be used to experimentally characterize and evaluate distributed realtime systems under fault-free and faulted conditions is described. A survey is presented of validation methodologies. The need for fault insertion based on validation methodologies is demonstrated. The origins and models of faults, and motivation for the FIAT concept are reviewed. FIAT employs a validation methodology which builds confidence in the system through first providing a baseline of fault-free performance data and then characterizing the behavior of the system with faults present. Fault insertion is accomplished through software and allows faults or the manifestation of faults to be inserted by either seeding faults into memory or triggering error detection mechanisms. FIAT is capable of emulating a variety of fault-tolerant strategies and architectures, can monitor system activity, and can automatically orchestrate experiments involving insertion of faults. There is a common system interface which allows ease of use to decrease experiment development and run time. Fault models chosen for experiments on FIAT have generated system responses which parallel those observed in real systems under faulty conditions. These capabilities are shown by two example experiments each using a different fault-tolerance strategy.

Czeck, Edward W.↗
NASA NTRS2007

Trajectory Design and Orbit Determination for the Lunar CRater Observation and Sensing Satellite (LCROSS)

The Lunar CRater Observation and Sensing Satellite (LCROSS) was competitively selected by the National Aeronautical and Space Administration (NASA) Exploration Systems Mission Directorate (ESMD) as a low-cost (< $80M) 1000 kg secondary payload to be launched with the Lunar Reconnaissance Orbiter (LRO) in October of 2008. LCROSS is a lunar impactor mission that will investigate the presence or absence of water in a permanently shadowed crater. Following launch, trans-lunar injection (TLI) and separation from LRO, LCROSS will remain attached to the launch vehicle's approximately 2300 kg spent Earth Departure Upper Stage (EDUS) and will guide it toward an impact of a permanently shadowed crater at the lunar South Pole. Hours prior to impact, LCROSS will separate from the EDUS and perform a braking maneuver that will allow the spacecraft to take measurements of the resulting EDUS impact ejecta cloud for several minutes, before impacting the crater as well. As a cost-capped secondary mission that must accommodate specific LRO launch dates, LCROSS faces unique challenges and constraints that must be carefully reconciled in order to satisfy an ambitious set of science observation requirements. This paper examines driving mission requirements and constraints and describes the trajectory design and navigation strategy that shape the LCROSS mission.

Galal, Ken↗
NASA NTRS1995

Influence of Geometry and Flow Variations on NO Formation in the Quick Mixer of a Staged Combustor

Staged combustion, such as Rich-Burn/Quick-Mix/Lean-Burn (RQL), is a viable strategy to meet nitric oxide (NO) emission goals for both stationary and propulsion gas turbine engines. A critical element of the design is the quick mixer section where the potential for NO production is high. While numerical calculations of the quick mixer under reacting conditions have been conducted, the hostile environment and lack of appropriate diagnostics have, to date, precluded experimental probing of the reacting case. As an alternative to understanding the effect of geometry and flow variations on the production of NO in the quick mixer, the present paper presents (1) a series of non-reacting parametric studies, and (2) a computational method to extrapolate the results of the non-reacting experiments to reacting conditions. The results show that the rate of NO production is highest in the immediate vicinity of the injection plane. For a given momentum flux ratio between the jets and mainstream, the most effective mixing geometry is that which mixes effectively in both (1) the plane of injection, and (2) the wall regions downstream of the plan of injection. The tailoring of the mixing is key to minimize the NO formed. As a result, the best overall mixer with respect to the minimization of NO production may depend on the system specific characteristics of the particular application.

Hatch, M. S.↗
NASA NTRS1999

A Theory of Oscillating Edge Flames

It has been known for some years that when a near-limit flame spreads over a liquid pool of fuel, the edge of the flame can oscillate relative to a frame moving with the mean speed. Each period of oscillation is characterized by long intervals of modest motion during which the edge gases radiate like those of a diffusion flame, punctuated by bursts of rapid advance during which the edge gases radiate like those in a deflagration. Substantial resources have been brought to bear on this issue within the microgravity program, both experimental and numerical. It is also known that when a near-asphyxiated candle-flame burns at zero gravity, the edge of the (hemispherical) flame can oscillate violently prior to extinction. Thus a web-surfer, turning to the NASA web-site at http://microgravity.msfc.nasa.gov, and following the trail combustion science/experiments/experimental results/candle flame, will find photographs and a description of candle burning experiments carried out on board both the Space-shuttle and the Russian space station Mir. A brief report can also be found in the proceedings of the Fourth Workshop. And recently, in a third microgravity program, the leading edge of the flame supported by injection of ethane through the porous surface of a plate over which air is blown has been found to oscillate when conditions are close to blow-off. A number of important points can be made with respect to these observations: It is the edge itself which oscillates, advancing and retreating, not the diffusion flame that trails behind the edge; oscillations only occur under near limit conditions; in each case the Lewis number of the fuel is significantly larger than 1; and because of the edge curvature, the heat losses from the reacting edge structure are larger than those from the trailing diffusion flame. We propose a general theory for these oscillations, invoking Occam's 'Law of Parsimony' in an expanded form, to wit: The same mechanism is responsible for the oscillations in all three experiments; and no new mechanism is invoked (Occam's original 'Razor'). Such a strategy eliminates Marangoni effects as the source, for these are absent in the second and third experiments. And it eliminates arguments that point to numerically predicted gas eddies as the source, a new mechanism, unelucidated. Indeed, we hypothesize that the essential driving mechanism for the instability is a combination of large Lewis number and heat losses from the reacting structure near the flame edge. Instabilities driven by these mechanisms are commonplace in 1D configurations. Chemical reactor theory, for example, leads to system responses which mimic the response of the candle flame - steady flame, oscillations, extinction. In a combustion context, oscillating instabilities were first reported for diffusion flames in a theoretical study by Kirkby and Schmitz, and here also the instabilities are associated with near-extinction conditions, large Lewis numbers, and heat losses. And deflagrations will oscillate if the Lewis number is large enough, oscillations that are exacerbated when heat losses are present, whether global or to a surface.

Buckmaster, J.↗
NASA NTRS2012

Launch and Assembly Reliability Analysis for Human Space Exploration Missions

NASA's future human space exploration strategy includes single and multi-launch missions to various destinations including cis-lunar space, near Earth objects such as asteroids, and ultimately Mars. Each campaign is being defined by Design Reference Missions (DRMs). Many of these missions are complex, requiring multiple launches and assembly of vehicles in orbit. Certain missions also have constrained departure windows to the destination. These factors raise concerns regarding the reliability of launching and assembling all required elements in time to support planned departure. This paper describes an integrated methodology for analyzing launch and assembly reliability in any single DRM or set of DRMs starting with flight hardware manufacturing and ending with final departure to the destination. A discrete event simulation is built for each DRM that includes the pertinent risk factors including, but not limited to: manufacturing completion; ground transportation; ground processing; launch countdown; ascent; rendezvous and docking, assembly, and orbital operations leading up to trans-destination-injection. Each reliability factor can be selectively activated or deactivated so that the most critical risk factors can be identified. This enables NASA to prioritize mitigation actions so as to improve mission success.

Cates, Grant↗
NASA NTRS1995

Two cis elements collaborate to spatially repress transcription from a sea urchin promoter

The expression pattern of many territory-specific genes in metazoan embryos is maintained by an active process of negative spatial regulation. However, the mechanism of this strategy of gene regulation is not well understood in any system. Here we show that reporter constructs containing regulatory sequence for the SM30-alpha gene of Stronglyocentrotus purpuratus are expressed in a pattern congruent with that of the endogenous SM30 gene(s), largely as a result of active transcriptional repression in cell lineages in which the gene is not normally expressed. Chloramphenicol acetyl transferase assays of deletion constructs from the 2600-bp upstream region showed that repressive elements were present in the region from -1628 to -300. In situ hybridization analysis showed that the spatial fidelity of expression was severely compromised when the region from -1628 to -300 was deleted. Two highly repetitive sequence motifs, (G/A/C)CCCCT and (T/C)(T/A/C)CTTTT(T/A/C), are present in the -1628 to -300 region. Representatives of these elements were analyzed by gel mobility shift experiments and were found to interact specifically with protein in crude nuclear extracts. When oligonucleotides containing either sequence element were co-injected with a correctly regulated reporter as potential competitors, the reporter was expressed in inappropriate cells. When composite oligonucleotides, containing both sequence elements, were fused to a misregulated reporter, the expression of the reporter in inappropriate cells was suppressed. Comparison of composite oligonucleotides with oligonucleotides containing single constituent elements show that both sequence elements are required for effective spatial regulation. Thus, both individual elements are required, but only a composite element containing both elements is sufficient to function as a tissue-specific repressive element.

Non-NASA Center↗
NASA NTRSDate not supplied

A Comparison of Stratospheric Smoke Events Seen by SAGE II (1984-2005) and SAGE III (2017-2021)

The ongoing Stratospheric Aerosol and Gas Experiment aboard the International Space Station (SAGE III/ISS) has mostly occurred during a period of relatively low aerosol loading. At the same time, it has almost observed a number of small to moderate injections of volcanic material in the stratosphere from the Aoba/Ambae eruption of 2018 to that of Karymsky in 2022. In addition, two of the largest known smoke intrusions into the stratosphere have occurred during the SAGE III/ISS mission (the British Columbia fire of August 2017 and the Australian Wildfire Event of 2019/2020). At least two other much smaller smoke events have also been observed in the SAGE III data (California Creek in September 2020 and McKay Creek in June 2021). Particularly with the two large events, it is tempting to speculate that these events are increasing in intensity and frequency possibly due to ongoing climate change. Realistically, change is difficult to assess since instrument data sets that can be used for quantifying historical events can be quite different in what and where they provide measurements. This complicates comparing one event to another potentially separated by many years. An exception to this issues are the measurements by SAGE III/ISS and those of its predecessor SAGE II (1984-2005). Both use the same measurement strategy, measure aerosol extinction coefficient at 1020 nm, and have similar spatial/temporal coverage. Both instruments are known to observe smoke events in the stratosphere. However, both have the same low observation density characteristic of solar occultation such that near-global coverage takes almost a month and characterizing effectively instant events like smoke and volcanic events take a matter of weeks depending on the vagaries of where the observations are being made at a critical juncture unlike instruments with high temporal/spatial resolution like limb-scatter instruments such as OMPS and OSIRIS. The goal of this work will be to use the same analysis approach to both the SAGE II and SAGE III data and compare both the frequency and intensity of smoke events observed by these instruments. While we do not expect to be able to make conclusions about change between the periods, the analysis is a first step in interpreting changes in the contribution of smoke to the stratospheric aerosol budget between the time frames encompassed by these instruments.

L. W. Thomason↗