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This report describes the regulatory bases for the 2025 revision of Army Regulation (AR) 50–7 - Nuclear and Chemical Weapons and Materiel: Army Reactor Program and the new associated Department of the Army pamphlet (DA PAM) Army Reactor Program Procedures.
Genome editing with sequence-specific nucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), is revolutionizing crop improvement. Developing efficient genome-editing protocols for highly polyploid crops, including sugarcane ( x = 10–13), remains challenging due to the high level of genetic redundancy in these plants. Here, we report the efficient multiallelic editing of magnesium chelatase subunit I ( MgCh ) in sugarcane. Magnesium chelatase is a key enzyme for chlorophyll biosynthesis. CRISPR/Cas9-mediated targeted co-mutagenesis of 49 copies/alleles of magnesium chelatase was confirmed via Sanger sequencing of cloned PCR amplicons. This resulted in severely reduced chlorophyll contents, which was scorable at the time of plant regeneration in the tissue culture. Heat treatment following the delivery of genome editing reagents elevated the editing frequency 2-fold and drastically promoted co-editing of multiple alleles, which proved necessary to create a phenotype that was visibly distinguishable from the wild type. Despite their yellow leaf color, the edited plants were established well in the soil and did not show noticeable growth retardation. This approach will facilitate the establishment of genome editing protocols for recalcitrant crops and support further optimization, including the evaluation of alternative RNA-guided nucleases to overcome the limitations of the protospacer adjacent motif (PAM) site or to develop novel delivery strategies for genome editing reagents.
The Vu Gia–Thu Bon (VG–TB) river basin is facing numerous challenges to water security, particularly in light of the increasing impacts of climate change. These challenges, including salinity intrusion, shifts in rainfall patterns, and reduced water supply in downstream areas, are of great concern. This study comprehensively assessed the current state of water security in the basin using robust statistical analysis methods such as the Process Analysis Method (PAM), SMART principle, and Analytic Hierarchy Process (AHP). This resulted in the development of a comprehensive assessment framework for water security in the VG–TB river basin. This framework identified five key dimensions, with basin development activities (0.32), the ability to meet water needs (0.24), and natural disaster resilience (0.19) being the most crucial and water resource potential being the least crucial (0.11) according to the AHP methodology. The latter also highlighted 15 indicators, four of which are particularly influential, including waste resources (0.54), flood (0.53), water storage capacity (0.45), and basin governance (0.42). Furthermore, 28 variables with high weight factors were identified. This framework aligns with the UN-Water water security definition and addresses the global water sustainability criteria outlined in Sustainable Development Goal 6 (SDG6). It enables the computation of a comprehensive Water Security Index (WSI) for specific regions, providing a strong foundation for decision-making and policy formulation. It aims to enhance water security in the context of climate change and support sustainable basin development, thereby guiding future research and policy decisions in water resource management.
Linear PAM regulator minimum energy design by geometric interpretation and graphical solution
Binary symmetrical channel noise effect on PCM PICTURE quality compared with white Gaussian noise effect in PAM system
Rapidly converging first order training algorithm for adaptive equalizer design in PAM signal reception, using variable step sizes for mean- square error minimization
A method of interleaving an audio signal with its associated video signal for simultaneous transmission or recording, and the subsequent separation of the two signals, is described. Comparisons are made between the new audio signal interleaving system and the Skylab Pam audio/video interleaving system, pointing out improvements gained by using the digital audio/video interleaving system. It was found that the digital technique is the simplest, most effective and most reliable method for interleaving audio and/or other types of data into the video signal for the Shuttle Orbiter application. Details of the design of a multiplexer capable of accommodating two basic data channels, each consisting of a single 31.5-kb/s digital bit stream are given. An adaptive slope delta modulation system is introduced to digitize audio signals, producing a high immunity of work intelligibility to channel errors, primarily due to the robust nature of the delta-modulation algorithm.
Consideration is given to the criticism of Nei and Tateno (1978) of the REH (random evolutionary hits) theory of genetic divergence in nucleic acids and proteins, and to their proposed alternative estimator of total fixed mutations designated X2. It is argued that the assumption of nonuniform amino acid or nucleotide substitution will necessarily increase REH estimates relative to those made for a model where each locus has an equal likelihood of fixing mutations, thus the resulting value will not be an overestimation. The relative values of X2 and measures calculated on the basis of the PAM and REH theories for the number of nucleotide substitutions necessary to explain a given number of observed amino acid differences between two homologous proteins are compared, and the smaller values of X2 are attributed to (1) a mathematical model based on the incorrect assumption that an entire structural gene is free to fix mutations and (2) the assumptions of different numbers of variable codons for the X2 and REH calculations. Results of a repeat of the computer simulations of Nei and Tateno are presented which, in contrast to the original results, confirm the REH theory. It is pointed out that while a negative correlation is observed between estimations of the fixation intensity per varion and the number of varions for a given pair of sequences, the correlation between the two fixation intensities and varion numbers of two different pairs of sequences need not be negative. Finally, REH theory is used to resolve a paradox concerning the high rate of covarion turnover and the nature of general function sites as permanent covarions.
Mars oblateness has been found to provide sun-synchronous orbits, including orbits with stationary apsides, similar to those used at earth. A low mass and low data rate complement of scientific instruments placed in such orbits can provide exciting planetary investigations such as the Mars Orbiter Water Mission described herein. Use of a modest Shuttle kickstage (PAM-A) and existing spacecraft hardware makes this mission low-cost. A preliminary mission and spacecraft design is described. The major emphasis of the paper is on the mechanics of heliocentric transfer for the 1986 and 1988 launch opportunities, Martian sun-synchronous orbit geometries, injectable mass capabilities, and methods of achieving these scientifically useful orbits.
The WESTAR-V, the second in a series of second-generation, large, 24-transponder communications satellites developed for the Space Communications Company is discussed. It is scheduled to be launched on a Delta vehicle from the Eastern Space and Missile Center no earlier than June 8, 1982. The launch support for this mission will be provided by NASA, on a reimbursable basis, to the Space Communications Company for a fixed price of $25.OM. The launch vehicle for the WESTAR-V mission will be the Delta 3910 configuration which incorporates an extended long tank Thor booster, nine Castor IV strap-on motors, a TR-201 second stage, and an 8-foot fairing. The Delta launch vehicle will place the spacecraft along a suborbital trajectory. The PAM-D stage will then thrust it to a synchronous transfer orbit. Three days after launch, the spacecraft apogee kick motor will be fired to circularize its orbit at geosynchronous altitude of 19,300 NM above the equator at approxmately 75 degrees west longitude.
On July 16, 1982 the successful launch of the Landsat-D spacecraft marked a significant step in the evolutionary growth of the Delta launch vehicle. This growth is related to the development of the Improved Second Stage (ISS). The second utilization of the ISS occurred August 26, 1982 with the successful launch of the Canadian Telesat-F communications satellite. Used in conjunction with the PAM third stage, this spacecraft was placed in a geosynchronous transfer orbit with extremely high accuracy. The various steps in the 22-year history of the Delta launch vehicle are briefly examined. In mid-1979 it became apparent that the expendable-to-Shuttle transition time would increase over that previously planned. In connection with this development, it was decided to design the ISS. Attention is given to design details, a system description, the development schedule, aspects of performance capability, first flight results, and future performance improvements.
The Canadiari Telesat-G (ANIK-D1) commercial communications satellite was launched successfully from the Eastern space and Missile Center (ESMC) at 7:10 p.m., EDT, on August 25, 1982, by a Delta 3920 Vehicle, Mission Number 164. Performance of the two stage Delta launch vehicle was nominal and placed the payload in a low circular orbit as planned. The Payload Assist Module (PAM-D), which is part of the payload, also performed nominally. The synchronous transfer orbital elements achieved by Delta/PAM, compared with the nominal expected, are provided. The satellite performed satisfactorily during the transfer orbit, and the ABM was fired successfully at 5:29 p.m., EDT, on August 29, 1982. The satellite was maneuvered to a position 104 degrees West Longitude above the equator. Satellite status is satisfactory and it has entered service.
The Mars Orbiter Water Mission (MOWM) is discussed as an example of a low-cost, low-risk focused-science Mars mission which might be implemented in the near term within recently imposed fiscal constraints. MOWM is designed to act as a follow-on to the Viking results in the areas of comparative climatology and water, carbon dioxide and dust inventories. The STS-compatible space vehicle would be based on proven earth-orbiting satellite hardware, fulfilling requirements for both spinning and despun components, operation with nonoptimum solar cell orientation, PAM-A upper stage compatibility, and data storage for delayed transmission. Study results have shown the technical and economic feasibility of missions based on any of four submitted designs which meet these requirements, and of low-cost, well-focused science objective Mars missions in general.
The physical laws governing launch vehicles are reviewed, together with the performance of launch vehicles thus far used and expected developments in space transportation systems. Launches are generally made in the direction of earth rotation to take advantage of the initial velocity in that direction, which can amount to about 0.46 km/sec at the equator. Spacecraft usually attain a parking orbit, where spacecraft conditions are assessed before moving on to final orbit or interplanetary trajectory. Rocket batteries were first used in a battle near Beijing in 1232, and manned space flight began with the Vostok flight in 1961, followed by the Apollo lunar landings in the later 1960s. Rocket thrust performance is analyzed, together with the thrust/mass ratio for ascent and descent. The Shuttle, capable of placing 29.5 t in LEO, will be or is equipped to also carry IUS and PAM engines for transferring payloads from LEO to GEO. Parallel burn boosters may be added to increase the payload capability, and Shuttle-derived launch vehicles may be developed to carry construction materials to space and return to earth for a runway landing. Alternatively, the Shuttle engines may be modularized in order to develop a heavy-lift launch vehicle for unmanned cargo ascents.
Payload and spacecraft check-out procedures followed at Kennedy Space Center (KSC) are described, and examples are furnished of Shuttle missions STS-5 and -6. Reliability must be assured in order to account for isolated operation in GEO once the satellites are released from the Orbiter. The spacecraft processing is handled in distinct flows that depend on the type of boost engine attached, e.g., the PAM-A, -D, or the IUS. Interface verification occurs in the Vehicle Processing Facility (VPF), which is equipped to store the payloads until an Orbiter is ready. Spacecraft are assigned to their respective processing lines by the Launch Site Support Manager. The STS-5 Orbiter was fitted with Getaway Specials, the SBS-C satellite, and the Anik-C spacecraft, and STS-6 carried the TDRSS-A spacecraft, which was mated to an IUS, three Getaway Specials, and two experiments for the pressurized cabin. The time necessary for preparing payloads is intended eventually to be reduced to 3 weeks.
The objective of this experiment is to determine the effects of long-term orbital exposure on the materials used in solid-rocket space motors. Specifically, structural materials and propellants from the STAR/PAM-D series motors and the PAM DII/IPSM-II motors will be tested, as well as advanced composite case and nozzle materials planned for future use. The experiment approach is to expose samples of solid-rocket propellant, liner, insulation, case, and nozzle specimens to the space environment and to compare preflight and postflight measurements of various mechanical, chemical, and ballistic properties.
The evolution of capabilities and the current performance levels of the Delta launch vehicle are outlined. The first payload was the Echo I passive communications satellite, weighing 179 lb, and placed in GEO in 1960. Emphasis since then has been to use off-the-shelf hardware where feasible. The latest version in the 3924 first stage, 3920 second stage, and Pam D apogee kick motor third stage. The Delta is presently equipped to place 2800 lb in GEO, as was proven with the 2717 lb Anik-D1 satellite. The GEO payload placement performance matches the Shuttle's, and work is therefore under way to enhance the Delta performance to handle more massive payloads. Installation of the Castor-IV solid motor separation system, thereby saving mass by utilizing compressed nitrogen, rather than mechanical thrusters to remove the strap-on boosters, is indicated, together with use of a higher performance propellant and a wider nose fairing.
A modularized, standardized spacecraft bus, known as MESA, suitable for a variety of science and applications missions is discussed. The basic bus consists of a simple structural arrangement housing attitude control, telemetry/command, electrical power, propulsion and thermal control subsystems. The general arrangement allows extensive subsystem adaptation to mission needs. Kits provide for the addition of tape recorders, increased power levels and propulsion growth. Both 3-axis and spin stabilized flight proven attitude control subsystems are available. The MESA bus can be launched on Ariane, as a secondary payload for low cost, or on the STS with a PAM-D or other suitable upper stage. Multi-spacecraft launches are possible with either booster. Launch vehicle integration is simple and cost-effective. The low cost of the MESA bus is achieved by the extensive utilization of existing subsystem design concepts and equipment, and efficient program management and test integration techniques.