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At least 109 records · Page 6

Characterization of Proteins in Filtrate from Biodegradation of Crop Residue

Biodegradation of plant biomass is a feasible path for transformation of crop residue and recycling of nutrients for crop growth. The need to model the effects of factors associated with recycling of plant biomass resulting from hydroponic sweet potato production has led to investigation of natural soil isolates with the capacity for starch hydrolysis. This study sought to use nondenaturing gel electrophoresis to characterize the proteins present in filtered effluent from bioreactors seeded with starch hydrolyzing bacterial culture used in the biodegradation of senesced sweet potato biomass. The study determined the relative molecular weight of proteins in sampled effluent and the protein banding pattern was characterized. The protein profiles of effluent were similar for samples taken from independent runs under similar conditions of starch hydrolysis. The method can be used as a quality control tool for confirmation of starch hydrolysis of crop biomass. In addition, this method will allow monitoring for presence of contaminants within the system-protein profiles indicative of new enzymes in the bioreactors.

Horton, Wileatha↗

Hydroponics Database and Handbook for the Advanced Life Support Test Bed

During the summer 1998, I did student assistance to Dr. Daniel J. Barta, chief plant growth expert at Johnson Space Center - NASA. We established the preliminary stages of a hydroponic crop growth database for the Advanced Life Support Systems Integration Test Bed, otherwise referred to as BIO-Plex (Biological Planetary Life Support Systems Test Complex). The database summarizes information from published technical papers by plant growth experts, and it includes bibliographical, environmental and harvest information based on plant growth under varying environmental conditions. I collected 84 lettuce entries, 14 soybean, 49 sweet potato, 16 wheat, 237 white potato, and 26 mix crop entries. The list will grow with the publication of new research. This database will be integrated with a search and systems analysis computer program that will cross-reference multiple parameters to determine optimum edible yield under varying parameters. Also, we have made preliminary effort to put together a crop handbook for BIO-Plex plant growth management. It will be a collection of information obtained from experts who provided recommendations on a particular crop's growing conditions. It includes bibliographic, environmental, nutrient solution, potential yield, harvest nutritional, and propagation procedure information. This handbook will stand as the baseline growth conditions for the first set of experiments in the BIO-Plex facility.

Allen J Nasb↗

Space Data for Crop Management

CROPIX, Inc., formed in 1984 by Frank Lamb, president of the Eastern Oregon Farming Company, monitors primarily potato crops in a 20,000 square mile area of northern Oregon and central Washington. Potatoes are a high value specialty crop that can be more profitable to the farmer if he has advance knowledge of market conditions, knows when to harvest, and when to take it to market. By processing and collecting data collected by the NASA-developed Landsat Earth Resources survey satellites, Lamb is able to provide accurate information on crop acreage and conditions on a more timely basis than the routine estimates by the USDA. CROPIX uses Landsat data to make acreage estimates of crops, and to calculate a field-by-field vegetative index number. CROPIX then distributes to its customers a booklet containing color-coded maps, an inventory of crops, plus data and graphs on crop conditions and other valuable information.

Source record↗

Composition and physical properties of starch in microgravity-grown plants

The effect of spaceflight on starch development in soybean (Glycine max L., BRIC-03) and potato (Solanum tuberosum, Astroculture-05) was compared with ground controls by biophysical and biochemical measurements. Starch grains from plants from both flights were on average 20-50% smaller in diameter than ground controls. The ratio delta X/delta rho (delta X --difference of magnetic susceptibilities, delta rho--difference of densities between starch and water) of starch grains was ca. 15% and 4% higher for space-grown soybean cotyledons and potato tubers, respectively, than in corresponding ground controls. Since the densities of particles were similar for all samples (1.36 to 1.38 g/cm3), the observed difference in delta X/delta rho was due to different magnetic susceptibilities and indicates modified composition of starch grains. In starch preparations from soybean cotyledons (BRIC-03) subjected to controlled enzymatic degradation with alpha-amylase for 24 hours, 77 +/- 6% of the starch from the flight cotyledons was degraded compared to 58 +/- 12% in ground controls. The amylose content in starch was also higher in space-grown tissues. The good correlation between the amylose content and delta X/delta rho suggests, that the magnetic susceptibility of starch grains is related to their amylose content. Since the seedlings from the BRIC-03 experiment showed elevated post-flight ethylene levels, material from another flight experiment (GENEX) which had normal levels of ethylene was examined and showed no difference to ground controls in size distribution, density, delta X/delta rho and amylose content. Therefore the role of ethylene appears to be more important for changes in starch metabolism than microgravity. c2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

NASA Experiment Number 9305014 2/2↗

Recycling crop residues for use in recirculating hydroponic crop production

As part of bioregenerative life support feasibility testing by NASA, crop residues are being used to resupply elemental nutrients to recirculating hydroponic crop production systems. Methods for recovering nutrients from crop residues have evolved from water soaking (leaching) to rapid aerobic bioreactor processing. Leaching residues recovered the majority of elements but it also recovered significant amounts of soluble organics. The high organic content of leachates was detrimental to plant growth. Aerobic bioreactor processing reduced the organic content ten-fold, which reduced or eliminated phytotoxic effects. Wheat and potato production studies were successful using effluents from reactors having with 8- to 1-day retention times. Aerobic bioreactor effluents supplied at least half of the crops elemental mass needs in these studies. Descriptions of leachate and effluent mineral content, biomass productivity, microbial activity, and nutrient budgets for potato and wheat are presented.

NASA Center KSC↗

Analysis of plant harvest indices for bioregenerative life support systems

Harvest indices, which are measures of the ratio of edible to total plant weight, are redefined to include edible sugars derived from enzymatic hydrolysis of the cellulose content of inedible plant components. Compositional analysis and carbohydrate contents of rapeseed, rice, soybeans, cowpea, wheat, sweet potato, white potato, and lettuce were analyzed to develop such generalized harvest indices. Cellulose conversion is shown to extend considerably the food available from plants otherwise grown for their oil and protein content in a bioregenerative life support system.

NASA Discipline Life Support Systems↗

Multiple forms of ADP-glucose pyrophosphorylase from tomato fruit

ADP-glucose pyrophosphorylase (AGP) was purified from tomato (Lycopersicon esculentum Mill.) fruit to apparent homogeneity. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis the enzyme migrated as two close bands with molecular weights of 50,000 and 51,000. Two-dimensional polyacrylamide gel electrophoresis analysis of the purified enzyme, however, revealed at least five major protein spots that could be distinguished by their slight differences in net charge and molecular weight. Whereas all of the spots were recognized by the antiserum raised against tomato fruit AGP holoenzyme, only three of them reacted strongly with antiserum raised against the potato tuber AGP large subunit, and the other two spots (with lower molecular weights) reacted specifically with antisera raised against spinach leaf AGP holoenzyme and the potato tuber AGP small subunit. The results suggest the existence of at least three isoforms of the AGP large subunit and two isoforms of the small subunit in tomato fruit in vivo. The native molecular mass of the enzyme determined by gel filtration was 220 +/- 10 kD, indicating a tetrameric structure for AGP from tomato fruit. The purified enzyme is very sensitive to 3-phosphoglycerate/inorganic phosphate regulation.

NASA Discipline Life Support Systems↗

Foods for a Mission to Mars: Equivalent System Mass and Development of a Multipurpose Small-Scale Seed Processor

The candidate crops for planetary food systems include: wheat, white and sweet potatoes, soybean, peanut, strawberry, dry bean including le ntil and pinto, radish, rice, lettuce, carrot, green onion, tomato, p eppers, spinach, and cabbage. Crops such as wheat, potatoes, soybean, peanut, dry bean, and rice can only be utilized after processing, while others are classified as ready-to-eat. To process foods in space, the food processing subsystem must be capable of producing a variety of nutritious, acceptable, and safe edible ingredients and food produ cts from pre-packaged and resupply foods as well as salad crops grown on the transit vehicle or other crops grown on planetary surfaces. D esigning, building, developing, and maintaining such a subsystem is b ound to many constraints and restrictions. The limited power supply, storage locations, variety of crops, crew time, need to minimize waste , and other equivalent system mass (ESM) parameters must be considere d in the selection of processing equipment and techniques.

Gandolph, J.↗

Process for producing vegetative and tuber growth regulator

A process of making a vegetative and tuber growth regulator. The vegetative and tuber growth regulator is made by growing potato plants in a recirculating hydroponic system for a sufficient time to produce the growth regulator. Also, the use of the vegetative and growth regulator on solanaceous plants, tuber forming plants and ornamental seedlings by contacting the roots or shoots of the plant with a sufficient amount of the growth regulator to regulate the growth of the plant and one more of canopy size, plant height, stem length, internode number and presence of tubers in fresh mass. Finally, a method for regulating the growth of potato plants using a recirculating hydroponic system is described.

Stutte, Gary W.↗

NASA's Contributions to Controlled Environment Agriculture

It may come as a surprise, but NASA has been a long-standing sponsor of controlled environment agriculture (CEA) research. This is based on the potential for using plants (crops) for life support systems in space. Through photosynthesis, crops could produce food and oxygen for humans, while removing CO2. In addition, plant transpiration could help purify waste water. NASAs interest in bioregenerative life support dates back to the late 1950s. At that time, much of the testing focused on algae, but over the years moved toward higher plants as CEA techniques improved. Throughout the 1980s and 90s, extensive testing was carried out at different universities to gather horticultural data for a range of crops, including wheat, soybean, lettuce, potato, sweet potato, cowpea, rice and more. These studies examined different electric light sources, mineral nutrition, recirculating hydroponics, effects of CO2, temperature, photosynthetic photon flux (PPF), and photoperiod on the crops, and identified cultivars that would be useful for space. Findings from these studies were then used to conduct large scale (20 sq m), closed atmosphere tests at Kennedy Space Center, and later at NASA Johnson Space Center, where plant growth chambers were linked to human habitats. Results showed that with high light input and careful horticultural management, about 20-25 sq m of crops under continuous cultivation could produce the O2 for one person, and about 40-50 sq m could produce enough dietary calories. The ability to sustain these production levels and accurately assess system costs and failures needs further study. In all likelihood, the use of plants for life support will evolve, where for early missions like the International Space Station, crops will be grown in small chambers to provide supplemental fresh foods. As mission durations and distances increase, the systems could expand to assume more of the life support burden. But the constraints of space travel require that these approaches be efficient in terms of mass, volume, and energy, which are similar to challenges facing terrestrial CEA, such as vertical agriculture systems.

Bioregenerative↗

Development of freeze dried vegetables

The development of freeze dried vegetables to be used in the Apollo food system is discussed. After the initial selection and screening of vegetables, several types of freeze dried vegetables were prepared in small batches. From these small batches, two vegetables were judged satisfactory for further testing and evaluation. These vegetables, mashed potatoes and asparagus, were subjected to storage at 100 deg plus or minus 5 F. for two weeks and then taste tested. The vegetables were also tested to determine if they complied with the microbiological requirements for Apollo food. The space food prototype production guide for the vegetables is submitted.

Larson, R. W.↗

Use of remote sensing in agriculture

Remote sensing studies in Virginia and Chesapeake Bay areas to investigate soil and plant conditions via remote sensing technology are reported ant the results given. Remote sensing techniques and interactions are also discussed. Specific studies on the effects of soil moisture and organic matter on energy reflection of extensively occurring Sassafras soils are discussed. Greenhouse and field studies investigating the effects of chlorophyll content of Irish potatoes on infrared reflection are presented. Selected ground truth and environmental monitoring data are shown in summary form. Practical demonstrations of remote sensing technology in agriculture are depicted and future use areas are delineated.

Pettry, D. E.↗

Crop identification and acreage measurement utilizing ERTS imagery

The Statistical Reporting Service of the U.S. Department of Agriculture is evaluating ERTS-1 imagery as a potential tool for estimating crop acreage. A main data source for the estimates is obtained by enumerating small land parcels that have been randomly selected from the total U.S. land area. These small parcels are being used as ground observations in this investigation. The test sites are located in Missouri, Kansas, Idaho, and South Dakota. The major crops of interest are wheat, cotton, corn, soybeans, sugar beets, potatoes, oats, alfalfa, and grain sorghum. Some of the crops are unique to a given site while others are common in two or three states. This provides an opportunity to observe crops grown under different conditions. Results for the Missouri test site are presented. Results of temporal overlays, unequal prior probabilities, and sample classifiers are discussed. The amount of improvement that each technique contributes is shown in terms of overall performance. The results show that useful information for making crop acreage estimates can be obtained from ERTS-1 data.

Wigton, W. H.↗

Evaluation of ERTS-1 data applications to geologic mapping, structural analysis and mineral resource inventory of South America with special emphasis on the Andes Mountain region

The author has identified the following significant results. A color composite of image E1010-14035, dated 2 August 1972, covers the west central Bolivian Altiplano near Salar de Coipasa. It clearly shows the distribution of surface water and scant patches of vegetation. The Salar de Coipasa is the largest body of water in the area, about 130 sq km of dark blue fresh water. A lighter blue area south of the lake suggests a thin cover of highly saline water superposed on salt beds. The scattered vegetation patches are presumed to be native grasses, lichens, and possibly Indian potato and maiz areas. A detailed study has been made of the scene which provides 12 different interpretive overlays including geology, volcanology, soils, hydrology, and relative permeability. It was found that color composites provide at least 40% more information that do black and white renditions. An excellent example of change detection was provided by image E1244-14051, dated 24 March 1973. Water in the Salar de Coipasa had more than doubled as a result of the rains of the Bolivian winter, which generally occur in the February-March period. The Salars are excellent and highly sensitive moisture indicators in this highly arid region.

Carter, W. D.↗

A distribution benefits model for improved information on worldwide crop production. Volume 2: Application to various crops

ECON's distribution benefits model has been applied to worldwide distribution of corn, rye, oats, barley, soybeans, and sugar, and to domestic distribution of potatoes. The results indicate that a LANDSAT system with thematic mapper might produce benefits to the United States of about $119 million per year, due to more efficient distribution of these commodities. The benefits to the rest of the world have also been calculated, with a breakdown between trade benefits and those associated with internal use patterns. By far the greatest part of the estimated benefits are assigned to corn, with smaller benefits assigned to soybeans and the small grains (rye, oats, and barley).

Andrews, J.↗

Plants for space plantations

Criteria for selection of candidate crops for closed life support systems are presented and discussed, and desired characteristics of candidate higher plant crops are given. Carbohydrate crops, which are most suitable, grown worldwide are listed and discussed. The sweet potato, ipomoea batatas Poir., is shown to meet the criteria to the greatest degree, and the criteria are recommended as suitable for initial evaluation of candidate higher plant crops for such systems.

Nikishanova, T. I.↗

Packaging's Contribution for the Effectiveness of the Space Station's Food Service Operation

Storage limitations will have a major effect on space station food service. For example: foods with low bulk density such as ice cream, bread, cake, standard type potato chips and other low density snacks, flaked cereals, etc., will exacerbate the problem of space limitations; package containers are inherently volume consuming and refuse creating; and the useful observation that the optimum package is no package at all leads to the tentative conclusion that the least amount of packaging per unit of food, consistent with storage, aesthetics, preservation, cleanliness, cost and disposal criteria, is the most practical food package for the space station. A series of trade offs may have to be made to arrive at the most appropriate package design for a particular type of food taking all the criteria into account. Some of these trade offs are: single serve vs. bulk; conventional oven vs. microwave oven; nonmetallic aseptically vs. non-aseptically packaged foods; and comparison of aseptic vs. nonaseptic food packages. The advantages and disadvantages are discussed.

Rausch, B. A.↗