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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 109 records · Page 6

Separation of life stages within anaerobic fungi (Neocallimastigomycota) highlights differences in global transcription and metabolism

Anaerobic gut fungi of the phylum Neocallimastigomycota are microbes proficient in valorizing low-cost but difficult-to-breakdown lignocellulosic plant biomass. Characterization of different fungal life stages and how they contribute to biomass breakdown are critical for biotechnological applications, yet we lack foundational knowledge about the transcriptional, metabolic, and enzyme secretion behavior of different life stages of anaerobic gut fungi: zoospores, germlings, immature thalli, and mature zoosporangia. A Miracloth-based technique was developed to enrich cell pellets with zoospores - the free-swimming, flagellated, young life stage of anaerobic gut fungi. By contrast, fungal mats contained relatively more vegetative, encysted, mature sporangia that form films. Global gene expression profiles were compared from two sample types (zoospore-enriched cell pellets vs. mature mats) harvested from the anaerobic gut fungal strain Neocallimastix californiae G1. Despite cultures being grown on glucose, the fungal zoospore-enriched samples were transcriptionally primed to encounter plant matter substrate, as evidenced by upregulation of catabolic carbohydrate-active enzymes and putative carbohydrate transporters. Furthermore, we report significant differential gene expression for gene annotation groups, including putative secondary metabolites and transcription factors. Understanding global gene expression differences between the fungal zoospore-enriched cells and mature fungi aid in characterizing fungal development, unmasking gene function, and guiding cultivation conditions and engineering targets to promote enzyme secretion.

59 BASIC BIOLOGICAL SCIENCES↗

Deep Learning for Fish Identification from Sonar Data (CRADA 481 Final Report)

In eastern regions of the United States, the American eel is a species of management and regulatory concern because of significant population declines, despite the species’ previous abundance in all tributaries of rivers flowing into the Atlantic Ocean. The American eel is also a candidate for listing under the U.S. Endangered Species Act. While hydropower construction and operation are only one of several factors contributing to this population decline, such a listing could impose additional regulatory challenges for a large number of hydropower projects. In this CRADA project, we improved technologies for identifying migrating eels with the goal of reducing the cost and time required for future American eel hydropower impact assessment and mitigation studies, while maintaining accuracy. We built on results from a previous FOA project (FOA# DE-FOA-0001662), led by the Electric Power Research Institute (EPRI), which developed a highly accurate, deep-learning method for identifying migrating eels from imaging sonar data. The current study aimed to further optimize this deep-learning model, originally designed for image classification, and to develop an object detection software capable of identifying fish from sonar videos in real time, enabling the detection of events like fish migrations and specific species, such as the American eel, at hydropower dams. The data conversion algorithms were packaged as software with a graphical user interface, and the software is evaluated by external collaborators. We focused on the American eel in this project and explored the transferability of the developed deep learning models to the sea lamprey, given the similar body shape and swimming behavior between the two species.

13 HYDRO ENERGY↗

On the dynamics of a human body model.

Equations of motion for a model of the human body are developed. Basically, the model consists of an elliptical cylinder representing the torso, together with a system of frustrums of elliptical cones representing the limbs. They are connected to the main body and each other by hinges and ball and socket joints. Vector, tensor, and matrix methods provide a systematic organization of the geometry. The equations of motion are developed from the principles of classical mechanics. The solution of these equations then provide the displacement and rotation of the main body when the external forces and relative limb motions are specified. Three simple example motions are studied to illustrate the method. The first is an analysis and comparison of simple lifting on the earth and the moon. The second is an elementary approach to underwater swimming, including both viscous and inertia effects. The third is an analysis of kicking motion and its effect upon a vertically suspended man such as a parachutist.

Huston, R. L.↗

Responses of blind fish to gravitational changes as achieved in parabolic flight

Blind fish, during parabolic flight, display a measurable and consistent behavior. The most spectacular new behavioral response is the forward looping of blind fish in or near weightlessness. This response shows no measurable adaptation during the entire period of weightlessness of about 30 sec. During the entrance and exit of weightless parabolas (pushover and pullout) respectively, the fish assumes a forward tilted diving position. Parabolic flight with negative g in the range between 0g and -1g causes similar diving responses of the fish with the only difference being that the dive is directed toward the top of the fish tank. When the response to a g value less than 1g is compared to the response to increased g load on the ground (escape of darting response) an essential difference is seen: higher horizontal acceleration or jerk on the ground causes fish to swim, or even dart, against the direction of inertial force; fish during weightless parabolas move into the direction of the inertial or gravitational force. Since the vestibular system of fish is homologous to that of man, the observed behavior of fish in weightless flight could help to better understand human performance and sensations in comparable situations.

Vonbaumgarten, R. J.↗

Study of the marine environment of the northern Gulf of California

The author has identified the following significant results. While occupying standard oceanographic stations in the delta region of the Colorado River, two species of insects were collected which were of special interest. One of these is a single specimen of corixid bug, hitherto familiar to the investigators only as a freshwater insect, but taken swimming actively in high salinity water along with characteristic marine plankton. Further investigation showed that the species taken is well known from saline ponds but this is apparently the first record of its occurrence in a complete marine community. A small note on this collection record was prepared for publication and sent in manuscript form to NASA. The other record is of three specimens of a cicindelid beetle (normally a beach inhabitant) found at a night light for larval fish collection on station in the Colorado delta. The beetles were observed to light on and take off from water surface while actively moving around the night light. There is an interesting question of whether this species (Cicindela sinaloae schrammeli Cazier) may be especially adapted for a predatory life on the surface film, while the other two (larger) species sympatric with it are restricted to hunting on land.

Hendrickson, J. R.↗

The rate of synthesis and decomposition of tissue proteins in hypokinesia and increased muscular activity

During hypokinesia and physical loading (swimming) of rats, the radioactivity of skeletal muscle, liver, kidney, heart, and blood proteins was determined after administration of radioactive amino acids. Tissue protein synthesis decreased during hypokinesia, and decomposition increased. Both synthesis and decomposition increased during physical loading, but anabolic processes predominated in the total tissue balance. The weights of the animals decreased in hypokinesia and increased during increased muscle activity.

Fedorov, I. V.↗

Determination of tropical cyclone surface pressure and winds from satellite microwave data

An approach to the problem of deducing wind speed and pressure around tropical cyclones is presented. The technique, called the Surface Wind Inference from Microwave data (SWIM technique, uses satellites microwave sounder data to measure upper tropospheric temperature anomalies which may then be related to surface pressure anomalies through the hydrostatic and radiative transfer equations. Surface pressure gradients outside of the radius of maximum wind are estimated for the first time. Future instruments may be able to estimate central pressure with + or - 0/1 kPa accuracy.

Kidder, S. Q.↗

Content of total iron, copper and manganese in liver of animals during hypokinesia, muscle activity and process of recovery

It is shown that the content of total iron, copper and manganese in the liver of animals is altered depending on the intensity and duration of their swimming. Hypodynamia for 7 days does not alter the concentration of iron, but sufficiently increases the content of copper and manganese. The barometric factor effectively influences the maintenance of constancy in the content of microelements accumulated in the liver after intensive muscle activity.

Potapovich, G. M.↗

Solar thermal heating and cooling. A bibliography with abstracts

This bibliographic series cites and abstracts the literature and technical papers on the heating and cooling of buildings with solar thermal energy. Over 650 citations are arranged in the following categories: space heating and cooling systems; space heating and cooling models; building energy conservation; architectural considerations, thermal load computations; thermal load measurements, domestic hot water, solar and atmospheric radiation, swimming pools; and economics.

Arenson, M.↗

Hydrodynamic role of fish squamosal integument as an analog of the surfaces directly formed by the turbulent flow. Report 2: Hydrodynamic function of squamosal integument

The stream flowing round the slowly swimming squama free fish can be laminized with the aid of the external slime coat alone. The slime of the fish with well developed squamae can laminize the stream together with the squamatic integument. Adjustments preventing a loss of the slime during laminization are better developed in the fastest squama free fishes.

Kudryashov, A. F.↗

Inflatable Column Structure

Lightweight structural member easy to store. Billowing between circumferential loops of fiber inflated column becomes series of cells. Each fiber subjected to same tension along entire length (though tension is different in different fibers). Member is called "isotensoid" column. Serves as jack for automobiles or structures during repairs. Also used as support for temporary bleachers or swimming pools.

Hedgepeth, J. M.↗

Effects of Weightlessness of Aurelia Ephyra Differentiation and Statolith Synthesis

Aurelia polyps are especially suited for space flight experiments because they are very small (2 to 4 mm), form ephyrae with gravity sensing structures in 6 to 7 days, and can be reared easily and inexpensively in the laboratory. During iodine-induced metamorphosis ephyrae develop in sequential order from the oral to the aboral end of the polyps. Eight sites of gravity receptors (rhopalia) form per ephyra. These structures have sacs of statoliths at their distal end, which are composed of calcium sulfate dihydrate. Only one statolith forms per cell (statocyte) and the cells collect at the distal end of the rhopalia forming statocysts. Rhopalia with statocysts are necessary for the righting reflex of swimming medusae. Using the Aurelia Metamorphosis Test System (Spangenberg, 1984) for the past eight months, the effects of clinostat rotation in the horizontal and verticall planes on the development of ephyrae and the synthesis of their statoliths were investigated.

Dorothy B. Spangenberg↗

Mechanism of colour discrimination by a bacterial sensory rhodopsin

A photosensitive protein resembling the visual pigments of invertebrates enables phototactic archaebacteria to distinguish color. This protein exists in two spectrally-distinct forms, one of which is a transient photoproduct of the other and each of which undergoes photochemical reactions controlling the cell's swimming behaviour. Activation of a single pigment molecule in the cell is sufficient to signal the flagellar motor. This signal-transduction mechanism makes evident a color-sensing capability inherent in the retinal/protein chromophore.

Spudich, J. L.↗

Retractable Sun Shade

Window-shade type spring roller contains blanket, taken up by rotating cylindrical frame and held by frame over area to be shaded. Blanket made of tough, opaque polyimide material. Readily unfurled by mechanism to protect space it encloses from Sun. Blanket forms arched canopy over space and allows full access to it from below. When shading not needed, retracted mechanism stores blanket compactly. Developed for protecting sensitive Space Shuttle payloads from direct sunlight while cargo-bay doors open. Adapted to shading of greenhouses, swimming pools, and boats.

Frank, A.↗

Energetics and optimum motion of oscillating lifting surfaces of finite span

In certain modes of animal propulsion in nature, such as bird flight and fish swimming, the efficiency compared to man-made vehicles is very high. In such cases, wing and tail motions are typically associated with relatively high Reynolds numbers, where viscous effects are confined to a thin boundary layer at the surface and a thin trailing wake. The propulsive forces, which are generated primarily by the inertial forces, can be calculated from potential-flow theory using linearized unsteady-wing theory (for small-amplitude oscillations). In the present study, a recently developed linearized, low-frequency, unsteady lifting-line theory is employed to calculate the (sectional and total) energetic quantities and optimum motion of an oscillating wing of finite span.

Ahmadi, A. R.↗

Amphibian fertilization and development in microgravity

An experiment investigating the effects of gravity on embryonic development in amphibians is proposed. The planned procedures for the preparation of the frog eggs for launching in the Space Shuttle, for the injection of the eggs with gonadotropin, for the insertion of the eggs into egg chambers, for the storage of one of the chambers in a microgravity area and the second into a centrifuge, and for the fertilization of the eggs are described. The later organogenesis, swimming behavior, cytoplasmic components, cellular formation, neural plate and archenteron expansion, and allometry and expansion of the organ systems will be examined. Normal morphology for embryos and tadpoles developing at microgravity and the formation of the neural plate opposite the sperm entry point meridian are predicted.

Souza, K. A.↗