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Kinematic Sensors Evaluation for Spaceflight Exercise Data Collections

INTRODUCTION: On the International Space Station (ISS), exercise feedback from astronauts is very important to diagnose and mitigate any form-related injuries and ensure efficacious exercise prescriptions and systems. Going forward, exploration exercise efforts seek to gain further quantitative data of human and system performance. Currently, methods of collecting in-flight exercise data on the ISS are limited to marker-based motion capture (MoCap) where astronauts must wear reflective markers over their clothes and specialized cameras are used. The main objective of this work was to investigate the following alternative tracking options: markerless video-based MoCap and inertial measurement units (IMUs). These were compared against traditional marker-based MoCap to evaluate kinematic accuracy and inform feasible methods for future exercise data collections on the ISS, especially in support of future Vibration Isolation and Stabilization (VIS) system development. METHODS: Three test subjects performed a variety of flight-like resistance and aerobic exercises using the Miniature Exercise Device (MED-2), Concept-2 rowing ergometer, barbell mockup, bench (e.g., for bench press, hip thruster, and cycling), and a custom structure for dips. These were intended also to represent exercises which could be performed on the multi-modality European Enhanced Exploration Exercise Device (E4D) [1]. The marker-based MoCap data, collected through a 16-camera OptiTrack MoCap system, was regarded as the gold standard to compare the data against. Passive markers were affixed to each subject according to a modified full body Plug-in Gait marker set [2] with 46 total markers. The markerless MoCap data was collected using two GoPro Hero7 cameras and one GoPro Hero11 camera. For the IMU data, a full body set of 17 Xsens DOTs were placed on the subject: 10 upper body and 7 lower body IMUs. Biomechanical modeling and evaluation was conducted through OpenSim [3] (MoCap), OpenSense [4] (IMU), OpenCap [5] (markerless), ENABLE [6] (markerless), and other modeling software. Secondary objectives included comparing the volume of equipment, reducing mass and crew set-up time. RESULTS AND DISCUSSION: While there were issues with initial processing for the IMUs and markerless MoCap, the results aided in the understanding of each sensor, developing end-to-end processes, and identifying future needs. Some observed concerns with the markerless MoCap approaches included being cognizant of a cluttered background, number of people in field of view, camera number and placement. Some challenges with the IMUs included possible sliding, early deactivation possibly due to exercise pose, and large quantity sensor synchronization. Overall, the markerless MoCap option may be the preferred method of data collection and processing as it provides a solution for certain IMU shortcomings and may be least in equipment volume, upmass, and crew setup time. CONCLUSIONS: While this work was mainly focused on ISS data collection, these sensor data along with continued evaluation and development efforts will help to establish best methods for exercise data collection on Gateway, for other Artemis missions, and beyond. Details on the latest end-to-end processing of the data and results will be presented, along with lessons learned and recommended sensor selection and methods.

S. Faragalla↗

Kinematic Sensors Evaluation for Spaceflight Exercise Data Collections

INTRODUCTION: On the International Space Station (ISS), exercise feedback from astronauts is very important to diagnose and mitigate any form-related injuries and ensure efficacious exercise prescriptions and systems. Going forward, exploration exercise efforts seek to gain further quantitative data of human and system performance. Currently, methods of collecting in-flight exercise data on the ISS are limited to marker-based motion capture (MoCap) where astronauts must wear reflective markers over their clothes and specialized cameras are used. The main objective of this work was to investigate the following alternative tracking options: markerless video-based MoCap and inertial measurement units (IMUs). These were compared against traditional marker-based MoCap to evaluate kinematic accuracy and inform feasible methods for future exercise data collections on the ISS, especially in support of future Vibration Isolation and Stabilization (VIS) system development. METHODS: Three test subjects performed a variety of flight-like resistance and aerobic exercises using the Miniature Exercise Device (MED-2), Concept-2 rowing ergometer, barbell mockup, bench (e.g., for bench press, hip thruster, and cycling), and a custom structure for dips. These were intended also to represent exercises which could be performed on the multi-modality European Enhanced Exploration Exercise Device (E4D) [1]. The marker-based MoCap data, collected through a 16-camera OptiTrack MoCap system, was regarded as the gold standard to compare the data against. Passive markers were affixed to each subject according to a modified full body Plug-in Gait marker set [2] with 46 total markers. The markerless MoCap data was collected using two GoPro Hero7 cameras and one GoPro Hero11 camera. For the IMU data, a full body set of 17 Xsens DOTs were placed on the subject: 10 upper body and 7 lower body IMUs. Biomechanical modeling and evaluation was conducted through OpenSim [3] (MoCap), OpenSense [4] (IMU), OpenCap [5] (markerless), ENABLE [6] (markerless), and other modeling software. Secondary objectives included comparing the volume of equipment, reducing mass and crew set-up time. RESULTS AND DISCUSSION: While there were issues with initial processing for the IMUs and markerless MoCap, the results aided in the understanding of each sensor, developing end-to-end processes, and identifying future needs. Some observed concerns with the markerless MoCap approaches included being cognizant of a cluttered background, number of people in field of view, camera number and placement. Some challenges with the IMUs included possible sliding, early deactivation possibly due to exercise pose, and large quantity sensor synchronization. Overall, the markerless MoCap option may be the preferred method of data collection and processing as it provides a solution for certain IMU shortcomings and may be least in equipment volume, upmass, and crew setup time. CONCLUSIONS: While this work was mainly focused on ISS data collection, these sensor data along with continued evaluation and development efforts will help to establish best methods for exercise data collection on Gateway, for other Artemis missions, and beyond. Details on the latest end-to-end processing of the data and results will be presented, along with lessons learned and recommended sensor selection and methods.

S Faragalla↗

Evaluation of the Terminal Area Precision Scheduling and Spacing System for Performance-Based Navigation Arrivals

The growth of global demand for air transportation has put increasing strain on the nation's air traffic management system. To relieve this strain, the International Civil Aviation Organization has urged all nations to adopt Performance-Based Navigation (PBN), which can help to reduce air traffic congestion, decrease aviation fuel consumption, and protect the environment. NASA has developed a Terminal Area Precision Scheduling and Spacing (TAPSS) system that can support increased use of PBN during periods of high traffic, while supporting fuel-efficient, continuous descent approaches. In the original development of this system, arrival aircraft are assigned fuel-efficient Area Navigation (RNAV) Standard Terminal Arrival Routes before their initial descent from cruise, with routing defined to a specific runway. The system also determines precise schedules for these aircraft that facilitate continuous descent through the assigned routes. To meet these schedules, controllers are given a set of advisory tools to precisely control aircraft. The TAPSS system has been evaluated in a series of human-in-the-loop (HITL) air traffic simulations during 2010 and 2011. Results indicated increased airport arrival throughput up to 10 over current operations, and maintained fuel-efficient aircraft decent profiles from the initial descent to landing with reduced controller workload. This paper focuses on results from a joint NASA and FAA HITL simulation conducted in 2012. Due to the FAA rollout of the advance terminal area PBN procedures at mid-sized airports first, the TAPSS system was modified to manage arrival aircraft as they entered Terminal Radar Approach Control (TRACON). Dallas-Love Field airport (DAL) was selected by the FAA as a representative mid-sized airport within a constrained TRACON airspace due to the close proximity of a major airport, in this case Dallas-Ft Worth International Airport, one of the busiest in the world. To address this constraint, RNAV routes and Required Navigation Performance with the particular capability known as Radius-to-Fix (RNP-RF) approaches to a short final were used. The purpose of this simulation was to get feedback on how current operations could benefit with the TAPSS system and also to evaluate the efficacy of the advisory tools to support the broader use of PBN in the US National Airspace System. For this NASA-FAA joint experiment, an Air Traffic Control laboratory at NASA Ames was arranged to simulate arrivals into DAL in Instrument Meteorological Conditions utilizing parallel dependent approaches, with two feeder positions that handed off traffic to one final position. Four FAA controllers participated, alternately covering these three positions. All participants were Full-Performance Level terminal controllers and members of the National Air Traffic Controllers Association. During the simulation, PBN arrival operations were compared and contrasted in three conditions. They were the Baseline, where none of the TAPSS systems TRACON controller decision support advisories were provided, the Limited Advisories, reflecting the existing but dormant capabilities of the current terminal automation equipment with providing a subset of the TAPSS systems advisories; numerical delay, landing sequence, and runway assignment information, and the Full Advisories, with providing the following in addition to the ones in the Limited condition; trajectory slot markers, timelines of estimated times of arrivals and sched

Performance based navigation↗

Zircon, Baddeleyite, and Reidite Found in Ries Crater Suevite

Introduction: Impact events can generate superheated impact melts and even vapor [1]. In the past decade, evidence of the high temperatures and high pressures of the impact process has been found in the impact melt from terrestrial craters (e.g., Mistastin Lake [2], Meteor Crater [3]) and even on the Moon [4–5]. Often, these studies involve zircon or zirconium-bearing phases. Zircon is a particularly useful mineral, due to its robustness and durability against weathering. It is used principally for chronology, but it has a multitude of geologic applications, including geothermometry and fingerprinting magma sources. Here we focus on its capability of recording impact conditions. Grains of zircon (ZrSiO4) are converted at high temperature and/or pressure during an impact event to reidite (a high-pressure polymorph of ZrSiO4) or to tetragonal-ZrO2 + SiO2 [6]. Reidite and tertragonal-ZrO2 leave identifying relicts in the rocks, markers of the high pressures and/or temperatures these rocks underwent. Ries Crater is a 26-km-diameter peak ring crater in southern Germany that formed approximately 15 Ma [7–9]. Ries is the type locality of the polymict impact breccia known as suevite. Here, we report a microanalytical study of suevite from the Ries impact structure. These analyses are used to inform our understanding of the pressure and temperature conditions involved in creating a polymict impact breccia such as suevite in a peak ring crater. Sample Description: The sample, denoted 16RS08, originates from Otting Quarry, at 48.8777° N, 10.7921° E, approximately 17 km from the center of the Ries Crater in Germany (approximately 4 km outside the crater rim). The material recorded in sample 16RS08 is therefore considered an outer suevite. The outer suevite is a discontinuous layer of polymict impact breccia that occurs outside the central ring of Ries, up to 22 km from the center of the crater [9]. Lithic clasts in the outer suevite consist primarily of crystalline basement rocks (gneiss, granite, amphibolite), with less than 5% of lithic clasts being overlying sedimentary rocks (limestone, sandstone, shale) [9]. The thin section studied contains variably shocked lithic and mineral clasts, impact glass, and interstitial minerals that make up the matrix of the breccia (Fig. 1) [9]. Methods: We used a Cameca SX100 electron probe microanalyzer (EPMA) located in the Kuiper Materials Imaging and Characterization Facility (KMICF) at the University of Arizona to obtain 15 elemental X-ray maps of 16RS08. We next used the JEOL 7900F SEM at the Astromaterials Research & Exploration Science (ARES) at NASA Johnson Space Center (JSC) to obtain electron backscatter diffraction (EBSD) maps and energy dispersive X-ray spectroscopy (EDS) maps of select portions of the section. The EBSD data were collected under beam conditions of 20 kV, and ~9 μA, with step sizes varying from 0.05 to 2 μm. Following EBSD data collection, we processed the data using AZtecCrystal and MTEX, a free MATLAB toolbox. Results: We used the elemental X-ray maps to identify the areas of interest in the section, particularly phosphates and Zr-bearing grains. These areas of interest were then targeted for follow up EBSD and EDS analyses. We have identified, through combined EBSD and EDS analysis, the presence of zircon, reidite, and monoclinic-ZrO2 (baddeleyite) in 16RS08. In 16RS08, we have found singular grains of zircon, zircon with a vermicular baddeleyite halo (Fig. 2a), and granular zircon with reidite (Fig. 2b). The different Zr-rich phases and their corresponding textures signifies that this sample underwent a broad spectrum of pressure and temperature conditions during the impact event. For example, zircons surrounded by a vermicular baddeleyite and SiO2 intergrowth (i.e., Fig. 2a) have been shown to preserve evidence of the extremely high temperatures of impact melt, upwards of 2370 °C [2, 6]. Similarly, reidite and granular zircon (i.e., Fig. 2b) have been shown to preserve evidence of high pressure, as the transition to reidite occurs >30 GPa [3, 6]. Future Work: Next, we will process the EBSD and EDS data for these Zr-rich grains, specifically looking for indicators of cubic- or tetragonal-ZrO2 in the baddeleyite remnants and of shock-precursors to the reidite. To further inform our work, we will also obtain BSE images of these grains using a Hitachi S-4800 SEM in KMICF at the University of Arizona, as well as geochemical spot analyses via EPMA. The data collected will be used to constrain the formation conditions of the Ries Crater outer suevite. Acknowledgments: We thank Ken Domanik and Jerry Chang for their support with data collection. This work was supported by a University of Arizona RII Core Facilities Pilot Program grant and start-up funds to JJB. TME thanks A. Cavosie and N. Timms for assistance during field sampling. We acknowledge support from NASA’s Planetary Science Research program for analysis performed at JSC. References: [1] Melosh H. J. (1989) Oxf. U. Press. [2] Timms et al. (2017) EPSL 477, 52–58. [3] Cavosie et al. (2016) Geology 44:9, 703–706. [4] White et al. (2020) Nature Astr. 4, 974–978. [5] Crow C. A. et al. (2017) GCA 202, 264–284. [6] Timms et al. (2017) Earth-Sci. Rev. 165, 185–202. [7] Schmieder M. et al. (2018) GCA 220, 146–157. [8] Schwarz W. H. et al. (2020) M&PS 55:2, 312–325. [9] Stöffler et al. (2013) M&PS 43:4, 515–589.

Zircon↗

Visualization of Microbial Biomarkers by Scanning Electron Microscopy

We are developing tools to link the biochemical structure of selected biomarkers with putative biogenic structures observed in mineralized samples. The detection of evidence of life on Mars and other planets will rely on methods that can discriminate compounds formed exclusively by living organisms. While biogenic compounds, such as amino acids and nucleotides have been discovered in extraterrestrial sources, such as meteorites and comets, their formation can be explained by abiotic means. The formation of cellular structures, or more elaborate organic molecules, such as complex lipids, proteins or nucleic acids, however, is strongly correlated to the presence of even the most primitive life processes. Recent evidence lends support to the hypothesis that life may have once existed on Mars. Carbonate globules and ppm concentrations of polycyclic aromatic hydrocarbons (PAHs) have been described in ALH84001, a meteorite originating from Mars ejecta captured by Earth over 13,000 years ago. The localized high concentration of PAHs that follow an increasing gradient from the intact fusion crust towards the interior corresponds to microgram quantities of hydrocarbon. Even though ALH84001 and other similar meteorites have withstood the forces capable of ejecting rock through Mars' escape velocity, upon entering Earth's atmosphere, their core temperatures are likely not to have been raised significantly, as evidenced by the survival of remanent magnetic signatures. Ideal biomarkers of ancient or modern biological life would include molecules that are (or were) pervasive and highly resistant to degradation. Also, requisite methods of detection should be simple, extremely sensitive and broadly inclusive (NASA SP-530). Lipopolysaccharide (LPS), peptidoglycan or pseudopeptidoglycan and beta-glucan are microbial cell wall components which together cover the entire microbial spectrum of eubacteria, archea and fungi. They are all remarkably resistant to thermal degradation. Fortunately, many antimicrobial defense systems of higher organisms require sensitive detection to combat microbial pathogens. We employ here the primitive immune system of the evolutionarily ancient horseshoe crab, Limulus polyphemus. This species relies on multi-enzyme signal amplification detection of cell wall molecules and they can be applied to the development of useful detectors of life. An extension of this work includes the visualization of microbial signatures by labeling LAL components with chromogenic or electron dense markers. The protein Limulus Anti-LPS Factor (LALF) has an extremely high affinity for LPS. By coupling LALF binding with colloidal gold labels we demonstrate a correlation of the structures visible by electron microscopy with biochemical evidence of microbial cell wall materials. Pure silica particles were mixed with cultures of E. coli (10(exp 6) cfu/mL). Samples were washed sequentially with buffered saline, LALF, antibody to LALF and finally colloidal gold-labeled Protein A. Negative controls were not exposed to E. coli but received identical treatment otherwise. Samples were coated with carbon and imaged on a JEOL JSM-840 scanning electron microscope with LaB6 source in the back scatter mode with the JEOL annular back scatter detector. 20 nm-scale black spots in this contrast-reversed image originate from electrons back-scattered by gold atoms. Negative controls did not give any signal. Future work will expand application of this technique to soil simulants and mineralized rock samples.

Wainwright, Norman R.↗