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Separation of isobaric Amino Acids and Small Molecule Metabolites Using Multipass Ion Mobility Analysis

Amino acids along with small molecule metabolites are important biomarkers for the study and detection of diseases that are initially analyzed in untargeted omics fashion. Amino acids and many metabolites are isomeric, and their specific form can have a significant impact on biological function. Chromatographic separation of isomers is challenging, and they cannot be resolved by mass spectrometry alone. Ion mobility is a technique that allows the separation of ions based on their size, shape, and charge. Here we present the results of the separation of isobaric amino acids and small molecule metabolites using a system that allows for multi-pass ion mobility separation which, in turn, enhances the ion mobility resolution of the separation. Amino acid standards and small molecule metabolites commercially available were infused directly into a SELECT SERIES™ Cyclic™ IMS system. Solutions of individual and mixture of the isobaric species were used and the mobility conditions were optimized for multiple passes for each corresponding set of isobaric species. Both ionization polarities and various solvent adducts were tested to provide the best signal intensity and separation. Amino acids such as leucine and isoleucine have previously been separated with ion mobility in a system with lower ion mobility resolution (SYNAPT™ G2 mass spectrometer) rendering about 90% of valley and mobility resolution of around 40 Ω/ΔΩ. With the enhanced ion mobility resolution using the cyclic IMS technique we have obtained almost complete separation of those amino acids rendering a valley of about 10% after 15 passes. For this case, the mobility resolution is around 250 Ω/ΔΩ. Another example is the separation of glucose-6-phosphate from glucose-1-phosphate, and fructose-6phosphate which were separated after 10 passes. For this case, the mobility resolution is around 205 Ω/ΔΩ.

Hernando J Olivos↗
NASA NTRS2011

A Three-Dimensional Receiver Operator Characteristic Surface Diagnostic Metric

Receiver Operator Characteristic (ROC) curves are commonly applied as metrics for quantifying the performance of binary fault detection systems. An ROC curve provides a visual representation of a detection system s True Positive Rate versus False Positive Rate sensitivity as the detection threshold is varied. The area under the curve provides a measure of fault detection performance independent of the applied detection threshold. While the standard ROC curve is well suited for quantifying binary fault detection performance, it is not suitable for quantifying the classification performance of multi-fault classification problems. Furthermore, it does not provide a measure of diagnostic latency. To address these shortcomings, a novel three-dimensional receiver operator characteristic (3D ROC) surface metric has been developed. This is done by generating and applying two separate curves: the standard ROC curve reflecting fault detection performance, and a second curve reflecting fault classification performance. A third dimension, diagnostic latency, is added giving rise to 3D ROC surfaces. Applying numerical integration techniques, the volumes under and between the surfaces are calculated to produce metrics of the diagnostic system s detection and classification performance. This paper will describe the 3D ROC surface metric in detail, and present an example of its application for quantifying the performance of aircraft engine gas path diagnostic methods. Metric limitations and potential enhancements are also discussed

Simon, Donald L.↗
NASA NTRS2014

Pilot Evaluation of a UAS Detect-and-Avoid System's Effectiveness in Remaining Well Clear

Unmanned aircraft will equip with a detect-and-avoid (DAA) system that enables them to comply with the requirement to "see and avoid" other aircraft, an important layer in the overall set of procedural, strategic and tactical separation methods designed to prevent mid-air collisions. Regulators will establish minimum operating standards for DAA effectiveness, but different combinations of algorithms, displays and procedures could be used to meet those standards. The research presented in this paper indicates the effectiveness of the combined pilot-DAA system as a function of the DAA design requirements and provides data that may be used to model the behavior of pilots when employing such systems. Two simulations involving 21 professional unmanned aircraft system (UAS) pilots evaluated eight different DAA system designs in order to assess their ability to maintain the "well clear" separation standard, i.e., the state of maintaining a safe distance from other aircraft that would not normally cause the initiation of a collision avoidance maneuver by either aircraft. When the traffic display was integrated with the primary mission map directly in front of the pilot, there were fewer losses of well clear. Greater warning time provided to the pilot was strongly correlated with success in remaining well clear. Pilots' ability to separate from aircraft with cooperative and non-cooperative surveillance systems was nearly the same after accounting for the amount of alert time provided in each encounter, although the limited surveillance volume for the airborne-equipped aircraft meant alerts tended to occur later and therefore were more difficult to resolve.

detect-and-avoid↗
NASA NTRS1979

Spectral methods for problems in complex geometries

Techniques that permit the efficient application of spectral methods to solve problems in nearly arbitrary geometries are presented. These methods were found to be viable alternatives to finite difference and finite element processes. The spectral methods applied are extensions of the standard techniques of separation of variables to the solution of arbitrarily complicated problems.

Orszag, S. A.↗
NASA NTRS1984

Using Ada for a distributed, fault tolerant system

It is pointed out that advanced avionics applications increasingly require underlying machine architectures which are damage and fault tolerant, and which provide access to distributed sensors, effectors and high-throughput computational resources. The Advanced Information Processing System (AIPS), sponsored by NASA, is to provide an architecture which can meet the considered requirements. Ada was selected for implementing the AIPS system software. Advantages of Ada are related to its provisions for real-time programming, error detection, modularity and separate compilation, and standardization and portability. Chief drawbacks of this language are currently limited availability and maturity of language implementations, and limited experience in applying the language to real-time applications. The present investigation is concerned with current plans for employing Ada in the design of the software for AIPS. Attention is given to an overview of AIPS, AIPS software services, and representative design issues in each of four major software categories.

Dewolf, J. B.↗
NASA NTRS1987

Static tests of the propulsion system

Advanced, highly-loaded, high-speed propellers, called propfans, are promising to revolutionize the transport aircraft industry by offering a 15- to 30-percent fuel savings over the most advanced turbofans without sacrificing passenger comfort or violating community noise standards. NASA Lewis Research Center and industry have been working jointly to develop the needed propfan technology. The NASA-funded Propfan Test Assessment (PTA) Program represents a key element of this joint program. In PTA, Lockheed-Georgia, working in concert with Hamilton Standard, Rohr Industries, Gulfstream Aerospace, and Allison, is developing a propfan propulsion system which will be mounted on the left wing of a modified Gulfstream GII aircraft and flight tested to verify the in-flight characteristics of a 9-foot diameter, single-rotation propfan. The propfan, called SR-7L, was designed and fabricated by Hamilton Standard under a separate NASA contract. Prior to flight testing, the PTA propulsion system was static tested at the Rohr Brown Field facility. In this test, propulsion system operational capability was verified and data was obtained on propfan structural response, system acoustic characteristics, and system performance. This paper reports on the results of the static tests.

Withers, C. C.↗
NASA NTRS1994

On the prediction of velocity fields from redshift space galaxy samples

We present a new method for recovering the underlying velocity field from an observed distribution of galaxies in redshift space. The method is based on a kinematic Zel'dovich relation between the velocity and density fields in redshift space. This relation is expressed in a differential equation slightly modified from the usual Poisson equation and which depends nontrivially on Beta identically equal to Omega(exp 0.6)/b. The linear equation can be readily solved by standard techniques of separation of variables by means of spherical harmonics. One can also include a term describing the 'rocket effect' discussed by Kaiser (1987). From this redshift space information alone, one can generate a prediction of the peculiar velocity field for each harmonic (l, m) as a function of distance. We note that for the quadrupole and higher order moments, the equation is a boundary value problem with solutions dependent on both the interior and exterior mass distribution. However, for a shell at distance r, the dipole, as well as the monopole, of the velocity field in the Local Group frame is fully determined by the interior mass distribution. This implies that the shear of the measured velocity field, when fitted to a dipole distortion, should be aligned and consistent with the gravity field inferred from the well determined local galaxy distribution. As a preliminary application we compute the velocity dipole of distant shells as predicted from the 1.2 Jy IRAS survey compared to the measured velocity dipole on shells, as inferred from a recent POTENT analysis. The coherence between the two fields is good, yielding a best estimate of Beta = 0.6 +/- 0.2.

Nusser, Adi↗
NASA NTRS1996

Simulator Evaluation of a New Cockpit Descent Procedure

An experiment was conducted to evaluate flight crew performance of the "Precision Descent," a new cockpit procedure designed to support the Descent Advisor (DA), one of the components in a new air traffic control advisory system called the "Center-TRACON Automation System" (CTAS). The DA predicts when aircraft will reach a specific waypoint on the arrival route, and presents controllers with clearance advisories designed to improve the sequencing of arriving aircraft. The effectiveness of the DA depends on the aircraft's descent trajectory: where it begins descent, what speed it maintains, how fast and at what altitude it crosses the bottom-of-descent waypoint. The Precision Descent allows controllers to assign these descent parameters to the flight crew. A Field Evaluation of the DA was conducted in Denver in 1995. Three separate clearances using standard ATC phraseology were used to support the cockpit descent procedure during this evaluation. The number and length of these clearances caused problems for both controllers and flight crews, causing readback errors, repeat requests and procedure misunderstandings. These observations led to a focus group meeting in which controller and pilot participants in the 1995 FE assisted in the redesign of the procedure. The Precision Descent eliminates one clearance used in the earlier study, and greatly reduces the length of the remaining clearances. This was accomplished by using non-standard clearance phraseology that relies on a published procedure chart for correct interpretation. Eight type-rated flight crews flew eight Precision Descents in a Boeing 747-400 simulator. No training was provided: crews received either a procedure chart or a procedure chart with a flight manual bulletin describing procedure techniques. Video and digital data were recorded for each descent. Preliminary results indicate that moving information from the verbal clearance to the chart was successful: the shorter clearances and the procedure's default crossing restriction were understood, and procedure compliance of 81% was observed across all descents on six independent compliance measures. Some readback errors and procedure misunderstandings were still observed, however; particularly when descent clearances deviated from the normative procedure.

Crane, Barry↗
NASA NTRS2010

Dispersions of Carbon nanotubes in Polymer Matrices

Dispersions of carbon nanotubes exhibiting long term stability are based on a polymer matrix having moieties therein which are capable of a donor-acceptor complexation with carbon nanotubes. The carbon nanotubes are introduced into the polymer matrix and separated therein by standard means. Nanocomposites produced from these dispersions are useful in the fabrication of structures, e.g., lightweight aerospace structures.

Wise, Kristopher Eric↗
NASA NTRS2019

The NASA Urban Air Mobility Testbed Flight Research Aircraft

The National Aeronautics and Space Administration (NASA) is leading government, industry and academic research effort known as Urban Air Mobility (UAM). The UAM activity goal is to develop the technology needed to make possible an urban air transportation system that makes use of human-crewed and crewless vehicles using automation, artificial intelligence and other technologies to safely and efficiently air transport people and goods within an urban environment. The NASA Langley Research Center (LaRC) created a UAM Flight Research Testbed Aircraft from a Cessna LC40 general aviation aircraft. The testbed has updated digital avionics and research systems needed to conduct flight research. The aircraft has two separate autopilots; a standard Federal Aviation Ad-ministration (FAA) certified system, and a modified research autopilot. The research autopilot has modifications that allow increased authority and enhancements to allow more automation and artificial intelligence controls. A network of three research computers hosts software to research several activities including automated air traffic sense and avoid, ground collision avoidance and obstacle avoidance. These UAM research activities involve automation and artificial intelligence technologies developed at three NASA centers. The NASA Langley, NASA Armstrong, and NASA Ames Research Centers are working together to develop and test these UAM technologies. This paper provides details of the systems, capabilities and research projects of the UAM Testbed Research Aircraft.

Howell, Charles T.↗
NASA NTRS2023

Safety Case for Small Uncrewed Aircraft Systems (sUAS) Beyond Visual Line of Sight (BVLOS) Operations at NASA Langley Research Center

This Technical Memorandum (TM) is written to provide for dissemination of the methods and safety considerations for operations of small Uncrewed Aerial Systems (sUAS) Beyond Visual Line-of-Sight (BVLOS)at NASA Langley Research Center. It includes the Safety Case used to acquire a BVLOS Certificate of Authorization (COA) from the FAA and is being published to enable others to benefit from this work. The intended operations, subject to approval from the Federal Aviation Administration (FAA) and the National Aeronautics and Space Administration (NASA), will include a combination of Within Visual Line of Sight (WVLOS) and Beyond Visual Line of Sight (BVLOS) flights, comprising of at most five sUAS operating concurrently, with no more than three operating BVLOS. Flights will occur in a subset of the Langley Air Force Base (LAFB) Class D airspace (KLFI) at a maximum altitude of 400 ft AGL. Most operations within this subset will take place in the City Environment Range Testing for Autonomous Integrated Navigation (CERTAIN) Range. The CERTAIN Range includes airspace inside the borders of NASA Langley Research Center (LaRC). Additional airspace over the northern section of CERTAIN will be requested as part of the Certificate of Authorization (COA). NASA LaRC BVLOS operations on the CERTAIN Range can be broken down into five critical components needed to meet the 14 CFR § 91.113 see and avoid requirement: 1) procedural deconfliction with LAFB for UAS operations at or below 400 ft and manned aircraft at or above 900’ AGL; 2) ground equipment for detection of intruder aircraft and to support communications between crewmembers ; 3) sUAS vehicles with advanced onboard automation capable of autonomously maintaining safe separation; 4) BVLOS standardized operating procedures (SOPs); 5) and personnel to execute the flight operations in accordance with the SOPs and respond to airborne contingencies. The introduction of new ground equipment includes the use of the Remote Operations for Autonomous Missions (ROAM) UAS Operations Center, development and use of an Integrated Airspace Display (IAD), use of the L-STAR and GA-9120 radars, and the incorporation of standardized Vertiports. The ROAM Operations Center will be the central point for all BVLOS sUAS operations. All command and control (C2), voice communications and airspace awareness displays will reside inside ROAM. The IAD will provide raw data from ADS-B, FLARM, radar tracks and telemetered GPS vehicle positions for interpretation by an Airspace Monitor. The radars will search the class D airspace around the CERTAIN Range and serve as a backup to procedural deconfliction procedures coordinated with LAFB. In the event of a procedural deconfliction breakdown, radar detections of non-participating aircraft will be available so that the 91.113 see and avoid requirement can still be safely met. Finally, the incorporation of Vertiports will have video and network connectivity that enables large numbers of sUAS launches and recoveries from a single location. This is a continuation of the remote command and control of unpiloted aircraft component focused on evaluating unpiloted aircraft flight crew roles and responsibilities, control interfaces and the associated data links needed to operate a fleet of aircraft within a UAM Ecosystem. This work supports the development of future aviation operational concepts based on an Urban Air Mobility Maturity Level (UML) 4 environment (Patterson, 2020). It is assumed that future airspace will include hundreds of simultaneous aircraft operations within the airspace, therefore scalable operations are essential for enabling this future airspace to become a reality. Follow on work includes envisioned flights that expand operations beyond the CERTAIN range and lead to an effective Maritime Surveillance capability.

Matthew W Coldsnow↗
NASA NTRS2017

Gradient Compression Garments as a Countermeasure to Post-Space Flight Orthostatic Intolerance: Potential Interactions with the Maximum Absorbency Garment

Astronauts and cosmonauts may experience symptoms of orthostatic intolerance during re-entry, landing, and for several days post-landing following short- and long-duration spaceflight. Presyncopal symptoms have been documented in approximately 20% of short-duration and greater than 60% of long-duration flyers on landing day specifically during 5-10 min of controlled (no countermeasures employed at the time of testing) stand tests or 80 deg head-up tilt tests. Current operational countermeasures to orthostatic intolerance include fluid loading prior to and whole body cooling during re-entry as well as compression garments that are worn during and for up to several days after landing. While both NASA and the Russian space program have utilized compression garments to protect astronauts and cosmonauts traveling on their respective vehicles, a "next-generation" gradient compression garment (GCG) has been developed and tested in collaboration with a commercial partner to support future space flight missions. Unlike previous compression garments used operationally by NASA that provide a single level of compression across only the calves, thighs, and lower abdomen, the GCG provides continuous coverage from the feet to below the pectoral muscles in a gradient fashion (from approximately 55 mm Hg at the feet to approximately 16 mmHg across the abdomen). The efficacy of the GCG has been demonstrated previously after a 14-d bed rest study without other countermeasures and after short-duration Space Shuttle missions. Currently the GCG is being tested during a stand test following long-duration missions (~6 months) to the International Space Station. While results to date have been promising, interactions of the GCG with other space suit components have not been examined. Specifically, it is unknown whether wearing the GCG over NASA's Maximum Absorbency Garment (MAG; absorbent briefs worn for the collection of urine and feces while suited during re-entry and landing) will interfere with the effectiveness of the GCG or conversely whether the GCG will reduce the fluid absorption capabilities of the MAG. Methods: This operational, directed study, will (1) determine whether the effectiveness of the GCG is affected by the MAG with regard to cardiovascular responses to head-up tilt, the standard orthostatic intolerance test employed for astronauts and bed rest subjects; (2) determine whether the effectiveness of the MAG is compromised by the GCG tested by injecting a standard fluid volume (950 ml in 3 separate simulated "urine voids") at a standardized rate (30 ml/sec); and (3) determine whether comfort is affected by wearing the MAG under the GCG using a standardized questionnaire. Results from this study will guide future development and operational use of the GCG and MAG to maximize crew health, safety, and comfort.

Lee, S. M. C.↗
NASA NTRS2013

Levitation Performance of Two Opposed Permanent Magnet Pole-Pair Separated Conical Bearingless Motors

In standard motor applications, rotor suspension with traditional mechanical bearings represents the most economical solution. However, in certain high performance applications, rotor suspension without contacting bearings is either required or highly beneficial. Examples include applications requiring very high speed or extreme environment operation, or with limited access for maintenance. This paper expands upon a novel bearingless motor concept, in which two motors with opposing conical air-gaps are used to achieve full five-axis levitation and rotation of the rotor. Force in this motor is created by deliberately leaving the motor s pole-pairs unconnected, which allows the creation of different d-axis flux in each pole pair. This flux imbalance is used to create lateral force. This approach is different than previous bearingless motor designs, which require separate windings for levitation and rotation. This paper examines the predicted and achieved suspension performance of a fully levitated prototype bearingless system.

Kascak, Peter↗
NASA NTRS2023

A Framework for Estimating Global River Discharge From the Surface Water and Ocean Topography Satellite Mission

The Surface Water and Ocean Topography (SWOT) mission will vastly expand measurements of global rivers, providing critical new data sets for both gaged and ungaged basins. SWOT discharge products (available approximately 1 year after launch) will provide discharge for all river that reaches wider than 100 m. In this paper, we describe how SWOT discharge produced and archived by the US and French space agencies will be computed from measurements of river water surface elevation, width, and slope and ancillary data, along with expected discharge accuracy. We present for the first time a complete estimate of the SWOT discharge uncertainty budget, with separate terms for random (standard error) and systematic (bias) uncertainty components in river discharge time series. We expect that discharge uncertainty will be less than 30% for two-thirds of global reaches and will be dominated by bias. Separate river discharge estimates will combine both SWOT and in situ data; these “gage-constrained” discharge estimates can be expected to have lower systematic uncertainty. Temporal variations in river discharge time series will be dominated by random error and are expected to be estimated within 15% for nearly all reaches, allowing accurate inference of event flow dynamics globally, including in ungaged basins. We believe this level of accuracy lays the groundwork for SWOT to enable breakthroughs in global hydrologic science.

Michael Durand↗
NASA NTRS1992

PHOTCAL: The IRAF photometric calibration package

The IRAF photmetric calibration package PHOTCAL is discussed. PHOTCAL is a set of tasks designed to derive the transformation from the instrumental photometric system to the standard photometric system, and apply the transformation to the observations. The PHOTCAL package contains tasks for: (1) creating and/or editing standard star catalogs and observations catalogs, (2) creating, checking and editing the configuration file which specifies the format of the standard star and observations catalogs and the form of the transformation equations, (3) solving the transformation equations interactively or non-iteractively using non-linear least squares fitting routines, and (4) applying the transformation to the observations. PHOTCAL standard star and observations catalogs are simple text files, whose columns are delimited by whitespace, and whose first column contains the star names. This format makes it relatively easy to interface the output of non-IRAF photometry programs as well as the output of the IRAF APPHOT and DAOPHOT photometry packages to PHOTCAL. PHOTCAL maintains a standard star catalog directory for the convenience of the user, but users can easily create their own standard star catalogs and/or define their own standard star catalog directory. Separate observations files produced by APPHOT, DAOPHOT or a user program containing data for stellar fields taken through different filters, can be combined into observations catalogs using one of the PHOTCAL preprocessor tasks. The input configuration file required by PHOTCAL is a text file, consisting of a series of instructions written by the user in a mini-language understood by the PHOTCAL parser. These instructions: (1) assign names to the input data columns in the standard star and observations catalogs, (2) assign names and initial values to the parameters to be fit, (3) define and describe how to solve the transformation equations. The mini-language approach permits great flexibility in the format of the input catalogs and the form of the transformation equations.

Davis, L. E.↗
NASA NTRS2002

ISAIA: Interoperable Systems for Archival Information Access

The ISAIA project was originally proposed in 1999 as a successor to the informal AstroBrowse project. AstroBrowse, which provided a data location service for astronomical archives and catalogs, was a first step toward data system integration and interoperability. The goals of ISAIA were ambitious: '...To develop an interdisciplinary data location and integration service for space science. Building upon existing data services and communications protocols, this service will allow users to transparently query hundreds or thousands of WWW-based resources (catalogs, data, computational resources, bibliographic references, etc.) from a single interface. The service will collect responses from various resources and integrate them in a seamless fashion for display and manipulation by the user.' Funding was approved only for a one-year pilot study, a decision that in retrospect was wise given the rapid changes in information technology in the past few years and the emergence of the Virtual Observatory initiatives in the US and worldwide. Indeed, the ISAIA pilot study was influential in shaping the science goals, system design, metadata standards, and technology choices for the virtual observatory. The ISAIA pilot project also helped to cement working relationships among the NASA data centers, US ground-based observatories, and international data centers. The ISAIA project was formed as a collaborative effort between thirteen institutions that provided data to astronomers, space physicists, and planetary scientists. Among the fruits we ultimately hoped would come from this project would be a central site on the Web that any space scientist could use to efficiently locate existing data relevant to a particular scientific question. Furthermore, we hoped that the needed technology would be general enough to allow smaller, more-focused community within space science could use the same technologies and standards to provide more specialized services. A major challenge to searching for data across a broad community is that information that describe some data products are either not relevant to other data or not applicable in the same way. Some previous metadata standard development efforts (e.g., in the earth science and library communities) have produced standards that are very large and difficult to support. To address this problem, we studied how a standard may be divided into separable pieces. Data providers that wish to participate in interoperable searches can support only those parts of the standard that are relevant to them. We prototyped a top-level metadata standard that was small and applicable to all space science data.

Hanisch, Robert J.↗
NASA NTRS1979

Cloud cover models derived from satellite radiation measurements

Using daily measurement of day and night infrared and incoming and absorbed solar radiation obtained from a TIROS satellite over a period of approximately 45 months, and integrated over 2.5 degree latitude-longitude grids, the proportion of cloud cover over each grid each day was derived for the entire period. For each of four three-month periods, estimates a and b of the two parameters of the best-fit beta distribution were obtained for each grid location. The (a,b) plane was divided into a number of regions. All the geographical locations whose (a,b) estimates were in the same region in the (a,b) plane were said to have the same cloud cover type for that season. For each season, the world was thus divided into separate cloud cover types. Using estimates of mean cloud cover for each season, the world was again divided into separate cloud cover types. The process was repeated for standard deviations. Thus for each season, three separate cloud cover models were obtained using the criteria of shape of frequency distribution, mean cloud cover, and variability of cloud cover. The cloud cover statistics were derived from once-a-day, near-local-noon satellite radiation measurements.

Bean, S. J.↗
NASA NTRS1984

The Rubidium-Crystal Oscillator Hybrid Development Program

The rubidium-crystal oscillator hybrid (RbXO) will make precise time available to systems that lack the power required by atomic frequency standards. The RbXO consists of two subassemblies in separate enclosures. One contains a small rubidium frequency standard (RFS) without its internal oven-controlled crystal oscillator (OCXO), plus interface circuits. The second contains a low-power OCXO, and additional interface circuits. The OCXO is on continuously. Periodically, e.g., once a week, the user system applies power to the RFS. After the few necessary for the warmup of the RFS, the interface circuits adjust the frequency of the OCXO to the RFS reference, then shut off the RFS. The OCXO enclosure is separable from the RFS enclosure so that manpacks will be able to operate with minimum size, weight, and power consumption, while having the accuracy of the RFS for the duration of a mission. A prototype RbXO's RFS has operated successfully for 4200 on-off cycles. Parallel efforts on a Phase 2 RbXO development are in progress. Two sources for the RbXO are scheduled to be available during 1986.

Vig, J. R.↗