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DOE OSTI2021

Smart Monitoring and Diagnostic System (SMDS) for Packaged Air Conditioners and Heat Pumps for Small/Medium Commercial Buildings: Preparation for Commercialization: CRADA 478 [Abstract only]

This project will enable Pacific Northwest National Laboratory (PNNL) and industry partner, mCloud Technologies, to work collaboratively to ready the Smart Monitoring and Diagnostic System (SMDS) for commercial deployment and validate its performance under real-world conditions at field sites. This project will specifically focus on 1) implementing the SMDS algorithms in a scalable cloud-based software architecture, 2) designing new, innovative, complimentary commercial services based on the SMDS, 3) enhancing the SMDS energy and cost impact algorithms to reduce uncertainty in estimates, 4) determining the lower limits on SMDS performance degradation detection, 5) validating algorithm performance and default values of adjustable thresholds with existing data from controlled physical testing and from customer packaged air conditioners and heat pumps (commonly referred to as rooftop units or RTUs), 6) field testing to validate the system on multiple customer buildings in diverse environments, and 7) expanding field deployment to a larger set of mCloud’s customer buildings. Project results by validating, enhancing, and quantifying the performance of the SMDS will position mCloud, and potential future licensees, to implement the SMDS in commercial offerings that encourage and enable use of condition-based and predictive maintenance, leading to significant reductions in energy use and greenhouse gas emissions associated with space conditioning by RTUs. Furthermore, these enhancements will increase the value of the SMDS for users and increase the potential market for its use and impacts.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

NASA NTRS2000

Mars Exploration 2003 to 2013 - An Integrated Perspective: Time Sequencing the Missions

The science goals for the Mars exploration program, together with the HEDS precursor environmental and technology needs, serve as a solid starting point for re-planning the program in an orderly way. Most recently, the community has recognized the significance of subsurface sampling as a key component in "following the water". Accessing samples from hundreds and even thousands of meters beneath the surface is a challenge that will call for technology development and for one or more demonstration missions. Recent mission failures and concerns about the complexity of the previously planned MSR missions indicate that, before we are ready to undertake sample return and deep sampling, the Mars exploration program needs to include: 1) technology development missions; and 2) basic landing site assessment missions. These precursor missions should demonstrate the capability for reliable & accurate soft landing and in situ propellant production. The precursor missions will need to carry out close-up site observations, ground-penetrating radar mapping from orbit and conduct seismic surveys. Clearly the programs should be planned as a single, continuous exploration effort. A prudent minimum list of missions, including surface rovers with ranges of more than 10 km, can be derived from the numerous goals and requirements; they can be sequenced in an orderly way to ensure that time is available to feed forward the results of the precursor missions. One such sequence of missions is proposed for the decade beginning in 2003.

Briggs, G.↗

DOE OSTI2022

Deciphering the structure of Arabidopsis thaliana 5-enol-pyruvyl-shikimate-3-phosphate synthase: An essential step toward the discovery of novel inhibitors to supersede glyphosate

Glyphosate interferes with plant aromatic metabolism through the inhibition of 5-enol-pyruvyl-shikimate-3-phosphate (EPSP) synthase [EPSPS, EC 2.5.1.19]. For this reason, EPSPS has been extensively studied in a vast array of organisms. This notwithstanding, up to date, the crystal structure of the protein has been solved exclusively in a few prokaryotes, while that of the plant enzyme has been only deduced in silico by similarity. This study aimed at determining the structure of EPSPS from the plant model species Arabidopsis thaliana, which has been cloned, heterologously expressed and affinity-purified. The kinetic properties of the enzyme have been determined, as well as its susceptibility to the inhibition brought about by glyphosate. The crystal structure of the protein has been resolved at high resolution (1.4 Å), showing open conformation of the enzyme, which is the state ready for substrate/inhibitor binding. This provides a framework for the structure-based design of novel EPSPS inhibitors. Surface regions near the active-site cleft entrance or at the interdomain hinge appear promising for inhibitor selectivity, while bound chloride near the active site is a potential placeholder for anionic moieties of future herbicides.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

COMET/ZEUS Activation Foils Report for Irradiations IER-241 and IER-261

The goal of this work was to determine the internal neutron spectrum of the COMET/ZEUS assembly using activation foils. Similar measurements were performed on the COMET assembly when it was located at the Los Alamos Critical Experiments Facility (LACEF) at the Los Alamos National Laboratory (LANL). The present measurements were performed after the COMET assembly was moved to the National Criticality Experiments Research Center (NCERC) located in the Device Assembly Facility (DAF) at the Nevada National Security Site (NNSS). The December 2012 COMET/ZEUS irradiation was the first in a series of experiments, and the COMET/ZEUS assembly was selected because of operational readiness and authorization to run specific targets. The August 2013 irradiation was the second in the series and involved only small changes in the configuration. This report describes the unfolding of the activation foils to determine the internal neutron spectra.

07 ISOTOPE AND RADIATION SOURCES↗

NASA NTRS2013

Integrating Wind Profiling Radars and Radiosonde Observations with Model Point Data to Develop a Decision Support Tool to Assess Upper-Level Winds for Space Launch

On the day-of-launch, the 45th Weather Squadron (45 WS) Launch Weather Officers (LWOs) monitor the upper-level winds for their launch customers to include NASA's Launch Services Program and NASA's Ground Systems Development and Operations Program. They currently do not have the capability to display and overlay profiles of upper-level observations and numerical weather prediction model forecasts. The LWOs requested the Applied Meteorology Unit (AMU) develop a tool in the form of a graphical user interface (GUI) that will allow them to plot upper-level wind speed and direction observations from the Kennedy Space Center (KSC) 50 MHz tropospheric wind profiling radar, KSC Shuttle Landing Facility 915 MHz boundary layer wind profiling radar and Cape Canaveral Air Force Station (CCAFS) Automated Meteorological Processing System (AMPS) radiosondes, and then overlay forecast wind profiles from the model point data including the North American Mesoscale (NAM) model, Rapid Refresh (RAP) model and Global Forecast System (GFS) model to assess the performance of these models. The AMU developed an Excel-based tool that provides an objective method for the LWOs to compare the model-forecast upper-level winds to the KSC wind profiling radars and CCAFS AMPS observations to assess the model potential to accurately forecast changes in the upperlevel profile through the launch count. The AMU wrote Excel Visual Basic for Applications (VBA) scripts to automatically retrieve model point data for CCAFS (XMR) from the Iowa State University Archive Data Server (http://mtarchive.qeol.iastate.edu) and the 50 MHz, 915 MHz and AMPS observations from the NASA/KSC Spaceport Weather Data Archive web site (http://trmm.ksc.nasa.gov). The AMU then developed code in Excel VBA to automatically ingest and format the observations and model point data in Excel to ready the data for generating Excel charts for the LWO's. The resulting charts allow the LWOs to independently initialize the three models 0-hour forecasts against the observations to determine which is the best performing model and then overlay the model forecasts on time-matched observations during the launch countdown to further assess the model performance and forecasts. This paper will demonstrate integration of observed and predicted atmospheric conditions into a decision support tool and demonstrate how the GUI is implemented in operations.

Bauman, William H., III↗

NASA NTRS2017

Mapping Application for Penguin Populations and Projected Dynamics (MAPPPD): Data and Tools for Dynamic Management and Decision Support

The Mapping Application for Penguin Populations and Projected Dynamics (MAPPPD) is a web-based, open access, decision-support tool designed to assist scientists, non-governmental organizations and policy-makers working to meet the management objectives as set forth by the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) and other components of the Antarctic Treaty System (ATS) (that is, Consultative Meetings and the ATS Committee on Environmental Protection). MAPPPD was designed specifically to complement existing efforts such as the CCAMLR Ecosystem Monitoring Program (CEMP) and the ATS site guidelines for visitors. The database underlying MAPPPD includes all publicly available (published and unpublished) count data on emperor, gentoo, Adelie) and chinstrap penguins in Antarctica. Penguin population models are used to assimilate available data into estimates of abundance for each site and year.Results are easily aggregated across multiple sites to obtain abundance estimates over any user-defined area of interest. A front end web interface located at www.penguinmap.com provides free and ready access to the most recent count and modelled data, and can act as a facilitator for data transfer between scientists and Antarctic stakeholders to help inform management decisions for the continent.

Humphries, G. R. W.↗

NASA NTRS2021

Practical Operational Readiness Gambits: Operations Training Simulations for the Curiosity Rover

The Mars Science Laboratory team had been puttingin effort to make a Training Venue to allow for parallel shadowtactical operations for trainees to actively work alongside theprime tactical operations personnel without affecting operations,since staffing constraints and shortened operations timelineswere straining the tactical process in supporting operationstrainees in the traditional way. The COVID- 19 Pandemic presentedfurther challenges in continuing on-console training forMSL operations trainees. The MSL operations team switched tofully remote operations, thus hampering the direct mentorshipa trainee would normally receive while on site at JPL. TheMars 2020 training team had developed a concept for roveroperations training simulations based on Johnson Space Center’sextensive simulations training program for astronauts andflight controllers. The MSL team borrowed this idea, and implementedthese training simulations which are called PracticalOperational Readiness Gambits (PORGs). The PORGs so farhave focused on the Science Planner and Rover Planner roles,which are two crucial roles in tactical operations that engagein key interactions throughout a shift. PORGs are based onactual sol scenarios that have occurred on Mars and follow thetactical operations process and timeline as closely as possible.However, unlike an operations shift, PORGs can slow down orpause to allow for more mentoring time. PORGs can focus onparticular skills to test the trainees on their understanding ofa concept. PORGs increase in complexity with each scenario toease the trainees into more typical tactical operations workloads.As more trainees join the MSL operations team, more roles arebeing incorporated into PORGs. There are plans to incorporatecertified operations personnel into PORGs to practice anomalyresponse situations. PORGs have become an essential part of theMSL operations training program and will continue even afterthe return to on-site operations at JPL.

Gajeway, Jocelyn↗

NASA NTRS2017

COBALT: Development of a Platform to Flight Test Lander GN&C Technologies on Suborbital Rockets

The NASA COBALT Project (CoOperative Blending of Autonomous Landing Technologies) is developing and integrating new precision-landing Guidance, Navigation and Control (GN&C) technologies, along with developing a terrestrial fight-test platform for Technology Readiness Level (TRL) maturation. The current technologies include a third- generation Navigation Doppler Lidar (NDL) sensor for ultra-precise velocity and line- of-site (LOS) range measurements, and the Lander Vision System (LVS) that provides passive-optical Terrain Relative Navigation (TRN) estimates of map-relative position. The COBALT platform is self contained and includes the NDL and LVS sensors, blending filter, a custom compute element, power unit, and communication system. The platform incorporates a structural frame that has been designed to integrate with the payload frame onboard the new Masten Xodiac vertical take-o, vertical landing (VTVL) terrestrial rocket vehicle. Ground integration and testing is underway, and terrestrial fight testing onboard Xodiac is planned for 2017 with two flight campaigns: one open-loop and one closed-loop.

Carson, John M., III↗

DOE OSTI2020

DOE Hanford Tank Side Cesium Removal System (TSCR) Update: Fabrication and Factory Acceptance Testing - 20300

The Tank Side Cesium Removal (TSCR) System is a modular, quickly-deployable, and passively safe system for efficiently removing solids and cesium from alkaline liquid waste raffinate created by fuel processing on the Hanford site. This paper provides an overview of the project with particular emphasis on completion of fabrication and factory acceptance testing (FAT). All TSCR System components are inside three enclosures known as the Process Enclosure, Ancillary Enclosure, and Control Enclosure. The Process Enclosure is seismic design category II (SDC-II) and built to withstand the rigors of transportation and lifting, high winds, volcanic ash fall, and heavy precipitation (snow) over a temperature range of -25 to 115 deg. F. The Process Enclosure and Control Enclosure were fabricated in AVANTech's Columbia, SC facility, while the Ancillary Enclosure was fabricated in AVANTech's Richland, WA facility. The design and preliminary documented safety analysis were completed in December 2019, with all work being performed in accordance with ASME NQA-1 quality assurance requirements. The Process Enclosure, Ion Exchange Columns (IXCs), and several other TSCR components have a nuclear safety function and are designated as safety significant structures, systems, and components (SS-SSCs). The NQA-1 Commercial Grade Dedication (CGD) process was used to identify critical characteristics in order to accept materials and items used for fabrication of several safety significant TSCR components. This paper also addresses unique aspects and challenges of implementing CGD in DOE facilities and systems. After fabrication and preliminary testing, the Process and Control Enclosures were shipped to AVANTech's Richland facility where the complete TSCR System was setup and connected in a represented layout and configuration that will be used on the Hanford site. Test tanks and pumps were put in place and connected to the TSCR Process Enclosure in a manner that mimicked interfacing Double Shell Tanks in the 241-AP Tank Farm. The TSCR System was then tested/operated under conditions equivalent to those planned for onsite operation. Testing was separated into five separate phases as defined below: - Phase 1: Confirmation of TSCR System readiness for the FAT; - Phase 2: Verification of TSCR System operation under standard conditions; - Phase 3: Demonstration of TSCR System operation under non-standard conditions; - Phase 4: Demonstration of ion exchange media removal from an IXC; and - Phase 5: Completion of TSCR System lift plans and site mobilization. The Hanford Tank Operations Contractor, Washington River Protection Solutions (WRPS), participated in all phases of testing. Testing at AVANTech's Richland Facility allowed WRPS personnel to familiarize themselves with TSCR operations in a radiologically clean facility prior to onsite mobilization. Engineering and operations personnel gained valuable experience that allowed them to better refine onsite operational protocols and procedures - including handling and movement of IXCs with a forklift. This paper describes the technologies used by the TSCR System as well as how these technologies are deployed and operated to advance the Hanford mission of safely, efficiently, and effectively treating tank waste. It also provides detailed information on fabrication and FAT, including operational data from simulated tank waste runs. A project schedule for WRPS' planned construction acceptance testing, operational acceptance testing, and actual tank waste treatment is provided. This presentation should be of great interest to parties responsible for the design, testing, and mobilization of modular NQA-1 technologies for the treatment of raffinate and similar liquid wastes containing high concentrations of radioactive cesium. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2025

Defense Technical Assistance - Infographic 4/24/25

Defense Technical Assistance (TA) Infographic designed to inform potential pilot partnerships with military installation about the TA opportunities available. - Secure and reliable energy is crucial for the operational readiness and resilience of military installations. The Department of Energy’s Grid Deployment Office has funded a new initiative at Idaho National Laboratory to conduct technical, on-site assessments that aim to secure the power infrastructure of military bases reliant on civilian utilities for their operations, both CONUS and OCONUS. Including how the assessments work and INL capabilities, partnership model as well as outcomes.

21 - SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLAN↗

DOE OSTI2024

FEED Study of CarbonCapture Inc DAC and CarbonCure Utilization Technologies Using United States Steel’s Gary Works Plant Waste Heat (Final Report)

The University of Illinois at Urbana-Champaign (UIUC) led this project to produce a front-end engineering design (FEED) study of an advanced Direct Air Capture and Utilization System (DACUS) system that can remove a minimum of 5,000 tonnes/yr net of carbon dioxide from air (based on cradle-to-gate LCA) and utilizing the CO 2 to produce low carbon intensity ready mix concrete. The designed system, if built, would be larger than any currently existing Direct Air Capture (DAC) collector in the U.S. Such carbon capture technologies are critical to meeting the goals of the DOE’s program to accelerate climate-critical technology. In addition to the power sector, industrial facilities for the manufacture of steel and cement/concrete are among the major sources of anthropogenic CO 2 . DAC is a promising new technology for reducing CO 2 , a potent greenhouse gas, in the atmosphere but is expensive, in part due to the energy required to adsorb and desorb captured CO 2 during cycles. By integrating CarbonCapture Inc. (CCI) DAC modules at United States Steel's Gary Works (USS) and utilizing the site's waste heat, energy, and location this project evaluates the feasibility of utilizing the captured CO 2 and the logistics of transportation. CarbonCapture Inc. has developed an innovative DAC system using novel adsorbents to cost-effectively capture CO 2 . The captured, liquified gas will be trucked to ready-mix concrete plants within the region, the closest of which is approximately 3.5 miles away, where CarbonCure will inject it into concrete during the mixing process at the facilities. The carbon dioxide reacts with concrete, mineralizing into calcium carbonate (CaCO 3 ), permanently locking the greenhouse gas into the matrix of the building material. This FEED study demonstrated a full CO 2 value chain for DACUS from industrial facilities. It also provided a means for Visage Energy Corp. (Visage) to assess the impact of this holistic approach on job creation, regional economic impact, and environmental justice. The project team also included Sargent & Lundy (S&L) to provide the constructability review and costing of the integration of the DAC with the steel plant. Ecotek Engineering USA, LLC designed the outside battery limit (OSBL) infrastructure to connect the DAC and the plant. Activities performed during the project included: (1) Project Management Plan; (2) Technology Maturation Plan (TMP); (3) Initial Workforce Readiness Plan; (4) Workforce Readiness Plan; (5) Front-End Engineering Design (FEED) Study; (6) Project Design Basis; (7) Hazards and Operability (HAZOP) Study; (8) Constructability Review; (9) Project Cost Assessment; (10) Logistics Analysis of CO 2 Transportation to the Utilization Site; (11) Business Case Analysis; (12) Life Cycle Analysis (LCA); (13) Environmental Health and Safety (EH&S) Analysis; (14) Environmental Justice Analysis; and (15) Economic Revitalization and Job Creation Outcomes Analysis. This report provides a summary of the outcomes and results of the project, which was performed between Oct. 1, 2022, through Sept. 30, 2024.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

NASA NTRS2018

Polar Volatiles Exploration in Peary Crater Enabled by NASA's Kilopower Project

For more than 50 years, scientists have discussed the possibility of the existence of water ice and other frozen volatiles at the lunar poles [1]. However, it was not until the 1990s when the polar orbiting spacecraft Clementine and Lunar Prospector collected data supporting these hypotheses [2]. Subsequent missions, including the Lunar Reconnaissance Orbiter (LRO) mission [3], and the Lunar Crater Observation and Sensing Satellite (LCROSS) mission [4], provided further evidence that supports the existence of water ice deposits at the lunar poles. During NASA's Constellation Program, several areas at both lunar poles polar were included in 50 Regions of Interest (ROI) for intensive study by the Lunar Reconnaissance Orbiter Camera (LROC) [5]. These polar ROI focused on peaks and craters rims that received high amounts of solar illumination, assuming initial missions back to the lunar surface would utilize solar arrays to generate electricity. Recently, the successful demonstration of NASA's Kilopower Project at the National Nuclear Security Administration (NNSA) Nevada National Security Site makes it possible to consider lunar polar missions at locations other than highly illuminated regions. The Kilopower Project was initiated in 2015 to demonstrate subsystem-level technology readiness of a small space fission power system [6]. This abstract describes the science objectives and operations for a mission concept developed at NASA Glenn Research Center's COMPASS Concurrent Engineering Team for a 1-year exploration of Peary Crater focused on prospecting for lunar polar volatiles.

Gruener, J. E.↗

DOE OSTI2020

Infrastructure and DFLAW Support at Hanford - 20443

Manhattan Project era infrastructure systems are degrading at an accelerated pace across the Department of Energy's (DOE) Environmental Management complex. Revitalizing, rejuvenating and right-sizing these systems to ensure reliability for ongoing cleanup missions is a major focus at the 580-square- mile Hanford Site in southeast Washington State. The push to complete construction, commissioning, startup, and operation of facilities and systems in the Direct-Feed Low-Activity Waste (DFLAW) program at Hanford by 2023 requires considerable coordination among site contractors and a significant investment in infrastructure. The passage of time also poses a challenge. It has been about 75 years since facilities were first operated at the site, and approximately 40 years of plutonium production created a legacy of unidentified active and abandoned underground obstacles and waste sites that must be avoided when building and upgrading the infrastructure. Converting infrastructure systems originally built to support plutonium production from the 1940's to the 1980's and upgrading those systems to optimize technology is an ever-changing balance of funding profiles, available resources and execution strategies. The DOE Richland Operations Office (RL) has long recognized the need for increased investment in Hanford Site infrastructure to support the future processing of approximately 56 million gallons of waste currently stored in large underground tanks. When DFLAW is fully operational, safe and reliable infrastructure systems will be needed to ensure continuity of operations around the clock, 365 days a year. These include roadways, water, power, sewer, information technology systems and facilities. To ensure the readiness of Hanford's infrastructure to support treating tank waste in the next three years, 15 projects were identified with a combined value of $133.8 million. Through calendar year 2019, 7 infrastructure projects have been substantially completed and the remaining 8 projects, with a remaining value of $103.8 million, are scheduled to be completed by December 2023. RL and its site services/infrastructure contractor, Mission Support Alliance (MSA), regularly evaluate the needs of the DFLAW program and other Hanford Site missions to ensure the highest priority systems are addressed. In addition to a fast-approaching deadline for round-the-clock treatment operations, RL and MSA face another significant infrastructure challenge. As cleanup is being completed in a 220-square-mile area called the River Corridor, most of the cleanup operations going forward will occur in a 20-square-mile area in the center of the Hanford Site, known as the Central Plateau. Infrastructure systems are becoming more congested in an already overcrowded area. This paper/presentation will outline some of the challenges Hanford faces while executing infrastructure reliability projects. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2025

Design of a High-Assay Low-Enriched Uranium Tri-Structural Isotropic Critical Experiment for Advanced Reactor Validation

High-assay low-enriched uranium (HALEU) fuel is a key component of many small modular reactor designs. Critical experiments are an important way to understand the neutronic performance of systems by obtaining nuclear data validations through measurements. Data reduce uncertainty and risk by showing that systems respond as predicted to changes such as temperature, subsequently advancing the overall technology readiness level of the materials within. Numerous critical experiments have been performed at the National Criticality Experiments Research Center (NCERC) operated by Los Alamos National Laboratory at the Nevada National Security Site since it became operational in 2011. However, the first experiment with HALEU fuel did not occur until 2024. Through extensive engineering, the experiment described in this paper was successfully designed and executed for the Comet vertical lift assembly at NCERC to perform measurements with HALEU tri-structural isotropic fuel that will assist in validation of nuclear data and computational modeling of small modular reactors for years to come.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

DOE OSTI2020

Play Fairway Analysis Retrospective GeoRePORTs

NREL, as part of the Play Fairway Analysis (PFA) Retrospective and with assistance from PFA PIs, completed GeoRePORTs for the sites identified in Phases 1&2 of the DOE PFA projects. The GeoRePORT (geothermal resource portfolio optimization and reporting technique) uses two factors to describe a specific project; grade and project readiness level. Grade describes the quality or potential of a project while project readiness level shows the progress of research and development. The categories that factor into the total grades and readiness levels are Geological, Technical, and Socio-Economic.

15 GEOTHERMAL ENERGY↗

NASA NTRS2005

Making a Reliable Actuator Faster and More Affordable

Before any rocket is allowed to fly and be used for a manned mission, it is first test-fired on a static test stand to verify its flight readiness. NASA s Stennis Space Center provides testing of Space Shuttle Main Engines, rocket propulsion systems, and related components with several test facilities. It has been NASA s test-launch site since 1961. The testing stations age with time and repeated use; and with aging comes maintenance; and with maintenance comes expense. NASA has been seeking ways to lower the cost of maintaining the stations, and has aided in the development of an improved reliable linear actuator that arrives onsite quickly and costs less money than other actuators. In general terms, a linear actuator is a servomechanism that supplies a measured amount of energy for the operation of another mechanical system. Accuracy, reliability, and speed of the actuator are critical to performance of the entire system, and these actuators are critical components of the engine test stands. Partnership An actuator was developed as part of a Dual-Use Cooperative Agreement between BAFCO, Inc., of Warminister, Pennsylvania, and Stennis. BAFCO identified four suppliers that manufactured actuator components that met the rigorous testing standards imposed by the Space Agency and then modified these components for application on the rocket test stands. In partnership with BAFCO, the existing commercial products size and weight were reworked, reducing cost and delivery time. Previously, these parts would cost between $20,000 and $22,000, but with the new process, they now run between $11,000 and $13,000, a substantial savings, considering NASA has already purchased over 120 of the units. Delivery time of the cost-saving actuators has also been cut from over 20 to 22 weeks to within 8 to 10 weeks. The redesigned actuator is commercially available, and the company is successfully supplying them to customers other than NASA.

Source record↗

DOE OSTI2024

Structure of saguaro cactus virus 3′ translational enhancer mimics 5′ cap for eIF4E binding

The genomes of several plant viruses contain RNA structures at their 3' ends called cap-independent translation enhancers (CITEs) that bind the host protein factors such as mRNA 5' cap-binding protein eIF4E for promoting cap-independent genome translation. However, the structural basis of such 5' cap-binding protein recognition by the uncapped RNA remains largely unknown. Here, we have determined the crystal structure of a 3' CITE, panicum mosaic virus-like translation enhancer (PTE) from the saguaro cactus virus (SCV), using a Fab crystallization chaperone. The PTE RNA folds into a three-way junction architecture with a pseudoknot between the purine-rich R domain and pyrimidine-rich Y domain, which organizes the overall structure to protrude out a specific guanine nucleotide, G18, from the R domain that comprises a major interaction site for the eIF4E binding. The superimposable crystal structures of the wild-type, G18A, G18C, and G18U mutants suggest that the PTE scaffold is preorganized with the flipped-out G18 ready to dock into the eIF4E 5' cap-binding pocket. The binding studies with wheat and human eIF4Es using gel electrophoresis and isothermal titration calorimetry, and molecular docking computation for the PTE–eIF4E complex demonstrated that the PTE structure essentially mimics the mRNA 5' cap for eIF4E binding. Such 5' cap mimicry by the uncapped and structured viral RNA highlights how viruses can exploit RNA structures to mimic the host protein-binding partners and bypass the canonical mechanisms for their genome translation, providing opportunities for a better understanding of virus-host interactions and non-canonical translation mechanisms found in many pathogenic RNA viruses.

3' cap-independent translation enhancers↗

DOE OSTI2025

Polar Bear™ – Innovative Capture of Storage Tank Vapors

Polar Bear™ is a patented technology developed by the Energy & Environmental Research Center (EERC) to capture storage tank vapors and eliminate methane emissions from upstream oil- and gas-producing facilities. Sparked by early commercial investment, the EERC licensed the technology and extended the intellectual property to storage tanks. Polar Bear™ is uniquely engineered and adapted to individual lower-producing facilities where there is otherwise no economic alternative for capturing tank vapors. A high number of small producing oil and gas wells are distributed across the country. The aggregate contributes to a significant volume of emissions. Because of the lack of economy of scale, gas volumes from these facilities are typically not recovered and contribute to methane emissions. Polar Bear™ provides a fit-for-purpose compression solution that addresses cost by reducing complexity with respect to conventional vapor recovery units and eliminating oil changes. Unique to Polar Bear™ is the capability to separate oxygenated gas from storage tank vapors. Storage tanks are designed to “breathe,” allowing gas to enter and escape during internal level and temperature changes. This infiltration of air into the tank headspace imparts undesirable oxygen content with respect to pipeline gathering. Polar Bear™ separates the vapor stream, allowing oxygen-rich gas to be used as fuel on-site while recovering the liquids-rich portion of the gas where oxygen content is minimized. A prototype system was tested to verify process models, evaluate operational performance, and advance the technology readiness level from 5 to 6. Results provide a good match between experimental measurements and process models, indicating the models are useful for future scale-up and field design. Various mixtures of nitrogen and liquefied petroleum gas were tested to understand the mass balance of nitrogen and how it relates to the potential control of oxygen content. Findings indicate that less than 2000 ppm of oxygen is likely to remain in the liquid portion of the gas in field applications. The research and development prepare the technology for field implementation to eliminate routine and fugitive methane emissions from storage tanks.

02 PETROLEUM↗