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Geophysical survey associated with NEON AOP survey, East River, CO 2018

The package contains data layers developed and used in Falco et al. 2024: “EcoImaging: Advanced Sensing to Investigate Plant and Abiotic Hierarchical Spatial Patterns in Mountainous Watersheds". The package is part of the DOE Watershed Function Science Focus Area (SFA) project and includes geophysical measurements collected at the East River, Colorado, in conjunction with the National Ecological Observatory Network (NEON) Airborne Observation Platform (AOP) survey conducted in June 2018. This dataset provide soil geophysical information and were used to investigate soil-plant relationships. The dataset consists of: - NEON_2018_EMI_survey.zip: the electromagnetic induction (EMI) survey as shape-file; - NEON_plot_TDR.csv: plot‑level data from Time‑Domain Reflectometry (TDR) measurements, providing: * volumetric water content (VWC) in percent (%); * soil temperature in degrees Celsius (°C); - file level metadata (flmd.csv) - data dictionary (dd.csv) file This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231.

2018 NEON and 2025 CHESS Campaigns↗

Evaluating the Persistence of Soil Carbon After a 30-Year Nitrogen Fertilization Experiment at the Fernow Experimental Forest, WV

We sampled soils in a previously nitrogen (N)-fertilized watershed (Watershed 3), which received 35 kg N per hectare annually for 30 years, and a reference watershed (Watershed 7), 4 years after the end of a 30-year N fertilization experiment at the Fernow Experimental Forest in West Virginia. As N deposition has reduced soil pH and plant carbon (C) investments into the rhizosphere, we compared the extent to which removing these potential limitations to microbial decomposition by increasing soil pH, adding artificial root exudates, or elevating soil temperature would increase microbial decomposition (and soil C losses) in soils that have and have not received excess N inputs. As such, we incubated soils with and without the addition of artificial root exudates and dolomitic lime (2 amendment treatments and 1 untreated control), at three different temperatures (15, 20, and 25 C) to simulate warming. We measured soil respiration, enzyme activity (acid phosphatase, N -acetyl-β-glucosaminidase, beta-glucosidase, phenol oxidase, and peroxidase), and microbial biomass carbon in a 15-week microcosm experiment. All of the datasheets include the metadata for all of the treatment combinations from this incubation experiment (ID, incubation temperature, treatment, watershed).The "Soil_Chemistry_Data.csv" data includes (1) the ratio of dry soil weight (g) by wet (or fresh) soil weight (g) after 72 hours at 65 C, (2) the wet (or fresh) weight of the soil samples used to determine soil pH, and (3) soil pH, which was measured in CaCl2. Detailed methods for the measurements of soil pH are described in the datasheet itself.The "Respiration_Data.csv" data includes (1) respiration day for the measurement, and (2) cumulative CO2 respiration (mmol) measured in the microcosms on specific days.The "Microbial_Biomass_Data.csv" data includes (1) mmol microbial biomass carbon per g (dry weight) of soil in each microcosm.The "Enzyme_Data.csv" data includes (1) all extracellular soil enzyme activity (N-acetyl-glucosaminidase (NAG), acid phosphatase (AP), β-glucosidase (BG), phenol oxidase and peroxidase) that we measured in our microcosms.

54 ENVIRONMENTAL SCIENCES↗

Kinetic Properties of Microbial Exoenzymes Vary With Soil Depth but Have Similar Temperature Sensitivities Through the Soil Profile

Current knowledge of the mechanisms driving soil organic matter (SOM) turnover and responses to warming is mainly limited to surface soils, although over 50% of global soil carbon is contained in subsoils. Deep soils have different physicochemical properties, nutrient inputs, and microbiomes, which may harbor distinct functional traits and lead to different SOM dynamics and temperature responses. We hypothesized that kinetic and thermal properties of soil exoenzymes, which mediate SOM depolymerization, vary with soil depth, reflecting microbial adaptation to distinct substrate and temperature regimes. We determined the Michaelis-Menten (MM) kinetics of three ubiquitous enzymes involved in carbon (C), nitrogen (N) and phosphorus (P) acquisition at six soil depths down to 90 cm at a temperate forest, and their temperature sensitivity based on Arrhenius/ Q 10 and Macromolecular Rate Theory (MMRT) models over six temperatures between 4–50°C. Maximal enzyme velocity ( V max ) decreased strongly with depth for all enzymes, both on a dry soil mass and a microbial biomass C basis, whereas their affinities increased, indicating adaptation to lower substrate availability. Surprisingly, microbial biomass-specific catalytic efficiencies also decreased with depth, except for the P-acquiring enzyme, indicating distinct nutrient demands at depth relative to microbial abundance. These results suggested that deep soil microbiomes encode enzymes with intrinsically lower turnover and/or produce less enzymes per cell, reflecting distinct life strategies. The relative kinetics between different enzymes also varied with depth, suggesting an increase in relative P demand with depth, or that phosphatases may be involved in C acquisition. V max and catalytic efficiency increased consistently with temperature for all enzymes, leading to overall higher SOM-decomposition potential, but enzyme temperature sensitivity was similar at all depths and between enzymes, based on both Arrhenius/ Q 10 and MMRT models. In a few cases, however, temperature affected differently the kinetic properties of distinct enzymes at discrete depths, suggesting that it may alter the relative depolymerization of different compounds. We show that soil exoenzyme kinetics may reflect intrinsic traits of microbiomes adapted to distinct soil depths, although their temperature sensitivity is remarkably uniform. These results improve our understanding of critical mechanisms underlying SOM dynamics and responses to changing temperatures through the soil profile.

59 BASIC BIOLOGICAL SCIENCES↗

Soil Moisture Data for TRACER project (Houston, TX)

Three soil moisture and metrological data collection sites are located in the Houston, TX, area and collect data every 5 minutes. The data includes soil moisture, volumetric wate content, electrical conductivity of soil, soil temperature, rain precipitation, air temperature and other parameters. For real-time streaming of the data please visit the website: https://coastal.beg.utexas.edu/soilmoisture/#!/

54 ENVIRONMENTAL SCIENCES↗

Forced Diffusion Chamber Monitoring of Soil CO2 Fluxes from 2019 to Present at NGEE Arctic Teller Site, Mile Marker 27, Alaska

Soil CO2 efflux was monitored at the NGEE Arctic Teller Site, Mile Marker 27, in Alaska. Monitoring was performed beginning in June 2019 using EOSense forced diffusion soil CO2 flux sensors at up to six sites characterized by varying vegetation cover, topographic position, and soil temperature and moisture. Dataset includes measurements of soil CO2 flux and chamber temperature. Time interval between measurements ranges from 5 minutes to 3 hours. Included are EOSense chamber locations as *.kml and *.png files, six *.csv files with measurements, and two *.csv reporting format files.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Throughfall-Reduction Drying Effects on Soil Respiration, Moisture, and Temperature in Four Distinct Lowland Panamanian Forests from 2015-2022

Objectives: Climatic drying is predicted for many tropical forests, yet effects on soil properties across moisture and soil gradients within tropical forests remain poorly characterized, hampering predictions of forest-climate feedbacks. We hypothesized that throughfall reduction (i.e. chronic drying) would suppress soil CO2 fluxes (i.e., respiration) in already-drier tropical forests by further reductions in soil moisture, but increases CO2 fluxes in wetter tropical forests by alleviating anaerobiosis and soil saturation. We conducted a field study in four distinct lowland Panamanian forests that vary in background rainfall and soil fertility. In each forest we constructed throughfall reduction structures that reduce moisture inputs to plots by ~50% (see methods). In drying and paired control plots we measured soil CO2 fluxes, soil moisture (volume/volume), soil temperature (°C), air temperature (°C), and forest floor biomass during wet-dry cycles (2015 – 2022). We also collected litterfall biomass and surveyed all tree species for diameter at breast height (dbh), and identified tree species in 2018 and 2019.Results:We found that soil moisture peaked in the wet season and declined in the dry season. Measured soil CO2 fluxes declined in the dry season and peaked in the early wet season ahead of peak soil moisture, resulting in a lower soil moisture optimum for respiration than previously modeled. Chronic throughfall exclusion also suppressed soil moisture across the four forests, and also initially suppressed soil CO2 fluxes across forests. There was sustained suppression of soil CO2 fluxes after four years in the wettest forest only (-28 ± 4% during the dry season), but elevated soil CO2 fluxes in a fertile forest after four years (+75 ± 28% during the late wet season). The unexpected negative drying effect in the wettest, most infertile forest could have resulted from reduced vertical flushing of nutrients into soils, as the drying effect increased with time. Including hydro-nutrient interactions in ecosystem models could improve predictions of tropical forest-climate feedbacks. Datasets included: Datasets included here include .csv and .xls files for soil respiration (carbon dioxide flux) collected using the LiCor (see methods), air temperature °C , soil moisture (vl/vl), and soil temperature °C. Soil moisture and soil temperature were collected at 0-10cm depths using hand-held probes (see methods). Data are on ~3 week timescales from 2015-2022 with some gaps. There is also a .kml file that includes coordinates for all 32 plots included in the study of four forests (n = 4 throughfall reduction and n = 4 control plots per site). No special software is needed to open these files.

54 ENVIRONMENTAL SCIENCES↗

Method used in Tokunaga, T. et al. 2018 for simulating time- and depth- dependent temperature variations in soil and sediment in Rifle, Colorado, 2013-2016.

This data package contains temperature, moisture content, matric potential, and carbon dioxide (CO2) concentration data from the Rifle, Colorado field site and from a 2 meter tall laboratory soil/sediment column designed to simulate field conditions. In addition, redox potential data from the laboratory column are included. The purpose of the data and paper is to demonstrate how a laboratory column was constructed to reproduce the dynamics of nonisothermal conditions in the field, such that CO2 production above a variable-depth water table can be studied in the laboratory. The data package contains 3 data files corresponding to the figures in the related paper (Tokunaga, T. et al. 2018) and 1 README file with the paper information.

54 ENVIRONMENTAL SCIENCES↗

Thermal Adaptation of Enzyme‐Mediated Processes Reduces Simulated Soil CO2 Fluxes Upon Soil Warming

Abstract Understanding factors influencing carbon effluxes from soils to the atmosphere is important in a world experiencing climatic change. Two important uncertainties related to soil organic carbon (SOC) stock responses to a changing climate are (a) whether soil microbial communities acclimate or adapt to changes in soil temperature and (b) how to represent this process in SOC models. To further explore these issues, we included thermal adaptation of enzyme‐mediated processes in a mechanistic SOC model (ReSOM) using the macromolecular rate theory. Thermal adaptation is defined here to encompass all potential responses of soil microbes and microbial communities following a change in temperature. To assess the effects of thermal adaptation of enzyme‐mediated processes on simulated SOC losses, ReSOM was applied to data collected from a 13‐year soil warming experiment. Results show that a model omitting thermal adaptation of enzyme‐mediated processes substantially overestimates observed CO 2 effluxes during the initial years of soil warming. The bias against observed CO 2 effluxes was lower for models including thermal adaptation of enzyme‐mediated processes. In addition, for a simulated linear 3°C soil warming over 100 years, models including thermal adaptation of enzyme‐mediated processes simulated SOC losses of a factor of three smaller than models omitting this process. As thermal adaptation of microbial community characteristics is generally not included in models simulating feedback between the soil, biosphere and atmosphere, we encourage future studies to assess the potential impact that microbial adaptation has on soil carbon – climate feedback representations in models. Plain Language Summary A major uncertainty in projecting how much soil organic carbon (SOC) will be converted to CO 2 as a consequence of climate change is related to how soil microbes may adapt to increasing soil temperatures. While this “microbial thermal adaptation” has been shown to occur in short‐term lab incubation experiments, its effect on SOC cycling on a decadal timescale is not clear. To address this knowledge gap, a mechanistic SOC model was used to simulate data collected from a 13‐year soil warming experiment, to assess how microbial thermal adaptation affects predicted SOC losses upon soil warming. The model results show that incorporating microbial thermal adaptation into the model led to reduced CO 2 effluxes from the soil to the atmosphere compared to the common approach of omitting this mechanism. Our results imply that projected SOC losses for the decades to come may be reduced when this mechanism is incorporated in land models. We therefore advocate for more research on the mechanisms controlling microbial thermal adaptation, and how to implement this mechanism in SOC models. Key Points A crucial aspect of soil organic carbon (SOC) models is the representation of soil microbes Predicted soil CO 2 fluxes upon soil warming are reduced when accounting for microbial thermal adaptation On a centennial time scale, this thermal adaptation results in up to a factor of three lower predicted SOC loss

Van de Broek, Marijn↗

Impact of fire on chemical properties and respiration of boreal forest soils

We collected both organic-rich and sandy soil cores from 12 sites (site_location_and_texture.csv) within Wood Buffalo National Park, Alberta, Canada. Laboratory burns were conducted by exposing intact soil cores to 60 kW m^-2 in a mass loss calorimeter to simulate boreal forest crown fires in order to measure the effects of burning (temp_data.csv) on soil properties including pH, total C, and total nitrogen (N) (metadata.csv). We used 70-day soil incubations and two-pool exponential decay models to characterize the impacts of burning and burn-induced changes in soil properties on soil respiration (soil_moisture_at_end_of_incubation.csv; soil_mass_at_end_of_incubation.csv; respiration_data.csv). Laboratory burns successfully captured a range of soil temperatures that were realistic for natural wildfire events.

54 ENVIRONMENTAL SCIENCES↗

Functional variability in specific root respiration translates to autotrophic differences in soil respiration in a temperate deciduous forest

CO 2 release from forest soils (R s ) is a prominent flux in the global carbon cycle. Rs is derived from roots (autotrophic respiration, R a ) and microbial (heterotrophic) respiration and is highly dynamic, as it depends on edaphic and environmental conditions as well as root functional traits and microbial community composition. It is unclear how root functional traits affect root and microbial respiration rates; however, their consideration may help parse out the relative contributions of root and microbial respiration to R s . At a temperate forest site, root systems of 3–4 functional root orders and their surrounding surface soil were carefully excavated and placed into custom trays designed to repeatedly measure R s in situ on eight temperate tree species that varied in their root functional strategies and mycorrhizal affinity. R s was measured bi-weekly to monthly for nearly one year using a custom chamber attached to a gas exchange system. R s varied over time, ranging from 0.3 to 12 µmol m -2 s -1 . Comparable root systems of the same species were excised from the soil and specific root respiration rates (R r ) were measured. Rr ranged from 2.5 to 9.0 nmol g -1 s -1 and was negatively correlated with root tissue density and positively related to root tissue nitrogen concentration. Using R r to estimate R a , we estimate that R a accounts for <10%, on average 2–3%, of R s for individual root systems (averaging 1.2 g dry biomass) housed in surrounding soil (average 1.3 kg dry mass) in situ; thus, Ra was roughly 20 times greater than Rh per unit mass. The contribution of R a peaked in the fall and coincided with leaf senescence of the forest canopy. A soil-sterilizing experimental treatment designed to help isolate R a in situ reduced bacterial biomass and shifted fungal community composition, but there was no reduction in Rs of the in-situ root-soil tray systems. The relative R a to R s ratio increased with root functional strategies characterized by greater specific root length and tip abundance, but also to greater root tissue density. The ratio of R a to R s also increased with warmer soil temperatures and decreased slightly with increasing soil moisture. We discuss how incorporating root functional traits as modulators of the autotrophic contribution to R s could be considered when modeling total soil CO 2 efflux from forests.

54 ENVIRONMENTAL SCIENCES↗

Seasonal Controls of CO 2 and CH 4 Dynamics in a Temporarily Flooded Subtropical Wetland

Subtropical and tropical wetlands play a prominent role in the global carbon (C) cycle; yet factors that influence their C fluxes remain uncertain. We collected measurements from a temporarily flooded subtropical wetland over 3 years to investigate environmental drivers impacting CO 2 and CH 4 fluxes. The wetland was a sink of CO 2 (-469 to -380 g C-CO 2 · m -2 · year -1 ) and a source of CH 4 (25.1 to 32.1 g C-CH 4 · m -2 · year -1 ) to the atmosphere. Dry season CH 4 emissions represented 41 to 49% of the annual budget, reflecting the importance of continuous CH 4 flux measurements. Gross primary productivity (GPP) increased with temperature and radiation, and the influence of VPD on GPP varied with soil inundation. Higher water tables decreased Reco and increased GPP, and a higher GPP in turn lead to enhanced R eco likely through enhancements of GPP on autotrophic respiration. This suggests that the impact of the water table on R eco depends on the cancelling effects of hydrology and GPP. Emissions of CH 4 increased with soil temperature, water table, and GPP until soils were inundated at which point temperature and GPP became the main drivers. Water table and temperature influenced GPP and CH 4 fluxes, and increases in GPP directly enhanced CH 4 emissions. In addition to impacting C fluxes directly through water table depth, hydrology also determined the hierarchy of the dominance of factors controlling C fluxes and their response. The positive climate forcing of subtropical wetlands may be dictated by plant-mediated and climate interactions, with hydrological factors playing a major role in determining the greenhouse gas sink or source strength of subtropical wetlands.

54 ENVIRONMENTAL SCIENCES↗

Data from: Coupled machine learning-ecosystem ensemble models substantially improve predictions of nitrous oxide (N 2 O) fluxes from US croplands

Nitrous oxide (N₂O) is a potent and persistent greenhouse gas, with rising atmospheric concentrations driven in part by inefficient use of synthetic nitrogen (N) fertilizers in agriculture. Predicting soil N₂O emissions is challenging due to high spatial and temporal variability arising from complex soil biogeochemical processes. Process-based ecosystem models and standalone machine learning (ML) approaches without extensive site-specific calibration often miss high emission episodes. Here, we show how an Ensemble Modeling System (EMS) based on outputs from an ensemble of ecosystem models coupled to an ensemble of ML models can improve predictions and understanding of N2O fluxes from US cropland. Trained and validated on approximately 12,000 N2O chamber measurements at 17 U.S. Midwest sites (six crops, 35 management practices), the EMS accurately predicted daily fluxes of N2O at both training (R² = 0.84, RMSE = 16.4 g N ha⁻¹ d⁻¹) and held-out testing sites (R² = 0.84, RMSE = 6.2 g N ha⁻¹ d⁻¹). Analyses identified six dominant N₂O drivers: soil organic carbon (SOC), NH₄⁺, NO₃⁻, water-filled pore space (WFPS), soil temperature, and biomass production. Wet, warm soils produced large N₂O peaks only with sufficient SOC and mineral N; in low-SOC soils, fluxes remained low. Incorporating these drivers into process-based models might significantly improve their predictive capacity. The EMS demonstrates a strong potential to predict N₂O fluxes at unseen sites, enabling more reliable regional inventories, improved gap-filling where measurements are sparse, and enhanced understanding of mechanisms to advance targeted mitigation strategies in food, feed, and bioenergy crops.

agricultural sciences↗

Hourly temperature and surface water level at the Ikpikpuk delta from 5/2012 to 11/2012

This data package includes the soil temperature and surface water level observations in 2012 at the Ikpikpuk River delta in northern Alaska. The data file consists of the observation table with both the .xlsx and .csv file formats. The files can be accessed through several software tools, like Microsoft Excel, and text editors. This is raw data from the near-surface ground temperature sensors at 15 cm (active layer) and 100 cm (permafrost) soil depth and a surface water sensor at the Ikpikpuk delta site in Alaska, US. The Ikpikpuk River delta is located on the Arctic Coastal Plain of northern Alaska. The Ikpikpuk River drains a land area of 15,330 km2, emptying into Smith Bay, and the delta is fan-shaped with an aerial extent of 106 km2 (Fuchs et al., 2018). This dataset supports the publication entitled "The thermal response of permafrost to coastal floodplain flooding" submitted to Environmental Research Letters (under review), where the observed water level is used as the upper boundary condition for the model simulations and the observed subsurface temperature is compared with the model simulations to answer the question about how coastal flooding affects permafrost thaw.

54 ENVIRONMENTAL SCIENCES↗

Low-Power, Flexible Sensor Arrays with Solderless Board-to-Board Connectors for Monitoring Soil Deformation and Temperature: Supporting Data

This dataset was used to assess the potential of a Soil Deformation and Temperature Monitoring System developed and presented in the article named "Low-Power, Flexible Sensor Arrays with Solderless Board-to-Board Connectors for Monitoring Soil Deformation and Temperature" and published in Sensors. There are 21 comma-delimited data files (.csv). 14 files contain current measurements performed in a lab setting, enabling the electrical evaluation of the sensor probe. These files are generated by the Keithley DMM6500 multimeter, and list the measured supply current (first column) as a function of time (3rd column). Another set of 6 files is also acquired in lab experiments, but contain soil temperature an deformation measurements, enabling an assessment of the developed device's accuracy. In these files, the first column contains the time (UTC), followed by the sensor's battery voltage and temperature and acceleration values (X, Y, Z) in subsequent columns. The measurements were acquired every 5 seconds. Another .csv file contains field data in a similar format (time, temperature, acceleration) collected at the Teller road (mile 27) site near Nome, Alaska with one probe. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Root and Microbial Soil CO 2 and CH 4 Fluxes Respond Differently to Seasonal and Episodic Environmental Changes in a Temperate Forest

Upland forest soils are typically major atmospheric carbon dioxide (CO 2 ) sources and methane (CH 4 ) sinks, but the contributions of root and microbial processes, as well as their separate temporal responses to environmental change, remain poorly understood. This 2-year study was conducted in a temperate, deciduous forest located on the Chesapeake Bay in Maryland, USA. We used temporal CO 2 and CH 4 flux measurements, exclusion-source partitioning, and an ecosystem-scale flooding experiment to understand how carbon (C) fluxes, and their root and microbial sources, respond to seasonal and episodic environmental change. Here, we show that the root-and-rhizosphere component of soil CO 2 and CH 4 flux is significant and that its dependence on soil temperature and volumetric water content (VWC) influences soil C dynamics at seasonal timescales. Experimental flooding shows that CO 2 and CH 4 flux responses to episodic moisture change were driven by suppression of soil heterotrophs, while root respiration did not respond to transient hydrologic disturbance. Methane uptake responded strongly to episodic inundation, reinforcing the important role of soil moisture in the short-term control of the forest soil CH 4 sink. However, despite the clear seasonality of CH 4 uptake, as well as its strong response to short-term experimental inundation, temperature and VWC were weak predictors of CH 4 uptake at a seasonal timescale. We suggest that CH 4 consumption in the long-term may be determined by vegetation, nutrients, microbial communities, or other factors correlated with seasonal changes. Our results indicate that root and microbial sources of both CO 2 and CH 4 flux respond differently in timing and magnitude to seasonal and episodic environmental change.

54 ENVIRONMENTAL SCIENCES↗

Active Layer and Moisture Measurements for Intensive Site 0 and 1, Utqiagvik (Barrow), Alaska

Thaw depth, relative dielectric permittivity, and soil temperature were collected along three transects within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) near Utqiagvik (Barrow), Alaska. Measurements were acquired along the transect at 3 m intervals on 2011-09-25, 2011-09-26, 2012-07-18, 2012-09-30, 2014-07-02, 2014-08-15, and 2014-09-14 for the L2 transect (intensive site 0), which is about 470 m long. Similarly, measurements were collected every 3 m on 2012-09-30, 2013-09-30, 2014-07-02, 2014-08-14, and 2014-09-23, and every 1 m on 2013-09-30 along the AB transect (intensive site 1) which is about 500 m long. File types in the package are all csv spreadsheets, following the ESS-DIVE csv guidelines (ESS-DIVE CSV v1), organized by transect name, with accompanying data dictionary files containing the filename suffix "_dd" (also csv format). Data collection details can be found in the Methods description of the metadata record. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Geophysical and Environmental Monitoring Data, Lower Watershed, Teller Road Mile Marker 27, Seward Peninsula, Alaska, 2017-2019

This data set contains geophysical and environmental monitoring data acquired between September 2017 and 2019 at the lower Teller watershed, Seward Peninsula, Alaska. Geophysical data comprises processed resistivity data, acquired daily between Spring and Fall of 2018 and 2019, and a baseline measurement of Fall 2017. The environmental monitoring data comprises depth resolved, distributed soil moisture and soil temperature data. These measurements were obtained used Decagon 5TE sensors placed at 0.1 m, 0.2 m , 0.3 m, and 0.4 m depth. In addition, temperature data at 0.5 m, 1.0 m, and 1.5 m were acquired using Hobo temperature sensors. Data were collected to improve our understanding of the hydrological response of discontinuous permafrost systems, in particular focusing on multi-annual dynamics and short term disturbances, such as snowmelt or precipitation events. The electrical resistivity tomography (ERT) monitoring data are included as processed resistivity models, with model cells x dimension equal to the distance along the profile, and model z dimension being elevation. The start and end point of the transect are (UTM Zone 3N): E 454881.66 m, N 7178949.45 m, and E 454772.60, N 7178885.81. Locations of the soil moisture and temperature sensors are provided in the data package. This dataset is discussed in detail in the Uhlemann, S. et al 2021 paper listed in the references. This dataset includes one *.pdf user guide and 315 *.csv data files included within four zipped files.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

Climate Sensitivity of Peatland Methane Emissions Mediated by Seasonal Hydrologic Dynamics

Peatlands are among the largest natural sources of atmospheric methane (CH 4 ) worldwide. Peatland emissions are projected to increase under climate change, as rising temperatures and shifting precipitation accelerate microbial metabolic pathways favorable for CH 4 production. However, how these changing environmental factors will impact peatland emissions over the long term remains unknown. Here, we investigate a novel data set spanning an exceptionally long 11 years to analyze the influence of soil temperature and water table elevation on peatland CH 4 emissions. We show that higher water tables dampen the springtime increases in CH 4 emissions as well as their subsequent decreases during late summer to fall. These results imply that any hydroclimatological changes in northern peatlands that shift seasonal water availability from winter to summer will increase annual CH 4 emissions, even if temperature remains unchanged. Furthermore, advancing hydrological understanding in peatland watersheds will be crucial for improving predictions of CH 4 emissions.

54 ENVIRONMENTAL SCIENCES↗