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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 109 records · Page 6

Transcriptional regulation of the raffinose family oligosaccharides pathway in Sorghum bicolor reveals potential roles in leaf sucrose transport and stem sucrose accumulation

Bioenergy sorghum hybrids are being developed with enhanced drought tolerance and high levels of stem sugars. Raffinose family oligosaccharides (RFOs) contribute to plant environmental stress tolerance, sugar storage, transport, and signaling. To better understand the role of RFOs in sorghum, genes involved in myo -inositol and RFO metabolism were identified and relative transcript abundance analyzed during development. Genes involved in RFO biosynthesis ( SbMIPS1, SbInsPase, SbGolS1, SbRS ) were more highly expressed in leaves compared to stems and roots, with peak expression early in the morning in leaves. SbGolS, SbRS, SbAGA1 and SbAGA2 were also expressed at high levels in the leaf collar and leaf sheath. In leaf blades, genes involved in myo -inositol biosynthesis ( SbMIPS1, SbInsPase ) were expressed in bundle sheath cells, whereas genes involved in galactinol and raffinose synthesis ( SbGolS1, SbRS ) were expressed in mesophyll cells. Furthermore, SbAGA1 and SbAGA2 , genes that encode neutral-alkaline alpha-galactosidases that hydrolyze raffinose, were differentially expressed in minor vein bundle sheath cells and major vein and mid-rib vascular and xylem parenchyma. This suggests that raffinose synthesized from sucrose and galactinol in mesophyll cells diffuses into vascular bundles where hydrolysis releases sucrose for long distance phloem transport. Increased expression (>20-fold) of SbAGA1 and SbAGA2 in stem storage pith parenchyma of sweet sorghum between floral initiation and grain maturity, and higher expression in sweet sorghum compared to grain sorghum, indicates these genes may play a key role in non-structural carbohydrate accumulation in stems.

60 APPLIED LIFE SCIENCES↗

Performance Comparison of Object Detection Networks for Shrapnel Identification in Ultrasound Images

Ultrasound imaging is a critical tool for triaging and diagnosing subjects but only if images can be properly interpreted. Unfortunately, in remote or military medicine situations, the expertise to interpret images can be lacking. Machine-learning image interpretation models that are explainable to the end user and deployable in real time with ultrasound equipment have the potential to solve this problem. We have previously shown how a YOLOv3 (You Only Look Once) object detection algorithm can be used for tracking shrapnel, artery, vein, and nerve fiber bundle features in a tissue phantom. However, real-time implementation of an object detection model requires optimizing model inference time. Here, we compare the performance of five different object detection deep-learning models with varying architectures and trainable parameters to determine which model is most suitable for this shrapnel-tracking ultrasound image application. We used a dataset of more than 16,000 ultrasound images from gelatin tissue phantoms containing artery, vein, nerve fiber, and shrapnel features for training and evaluating each model. Every object detection model surpassed 0.85 mean average precision except for the detection transformer model. Overall, the YOLOv7tiny model had the higher mean average precision and quickest inference time, making it the obvious model choice for this ultrasound imaging application. Other object detection models were overfitting the data as was determined by lower testing performance compared with higher training performance. In summary, the YOLOv7tiny object detection model had the best mean average precision and inference time and was selected as optimal for this application. Next steps will implement this object detection algorithm for real-time applications, an important next step in translating AI models for emergency and military medicine.

60 APPLIED LIFE SCIENCES↗

Few-Shot Learning Enables Population-Scale Analysis of Leaf Traits in Populus trichocarpa

Plant phenotyping is typically a time-consuming and expensive endeavor, requiring large groups of researchers to meticulously measure biologically relevant plant traits, and is the main bottleneck in understanding plant adaptation and the genetic architecture underlying complex traits at population scale. In this work, we address these challenges by leveraging few-shot learning with convolutional neural networks to segment the leaf body and visible venation of 2,906 Populus trichocarpa leaf images obtained in the field. In contrast to previous methods, our approach (a) does not require experimental or image preprocessing, (b) uses the raw RGB images at full resolution, and (c) requires very few samples for training (e.g., just 8 images for vein segmentation). Traits relating to leaf morphology and vein topology are extracted from the resulting segmentations using traditional open-source image-processing tools, validated using real-world physical measurements, and used to conduct a genome-wide association study to identify genes controlling the traits. In this way, the current work is designed to provide the plant phenotyping community with (a) methods for fast and accurate image-based feature extraction that require minimal training data and (b) a new population-scale dataset, including 68 different leaf phenotypes, for domain scientists and machine learning researchers. All of the few-shot learning code, data, and results are made publicly available.

59 BASIC BIOLOGICAL SCIENCES↗

Parathyroid Hormone, Calcitonin, and Vitamin D

Analyses of secretion of parathyroid hormone during tests of stimulation and suppression of hormone-secretory activity using infusions of EDTA and calcium, respectively, have established that, in contrast to previous views, secretion of the hormone is not autonomous in many patients that have adenomatous hyperparathyroidism, but is responsive to changes in blood-calcium concentration. These findings have led to a new understanding of the pathophysiology of hormone production in hyperparathy-roidism. A related application of the diagnostic use of the radioimmunoassay is the preoperative localization of parathyroid tumors and the distinction between adenomas and chief-cell hyperplasia. Work involving catheterization and radioimmunoassay of blood samples obtained from the subclavin and innominate veins and the venae cavae, led to localization in a high percentage of patients. However, this procedure has been adopted recently to detect hormone concentration in the small veins directly draining the parathyroid glands.

Potts, J. T.↗

Organic inclusions within hydrothermal minerals from S.W. Africa and elsewhere.

It was observed that quartz crystals from veins within a diabase dike of precambrian age from S.W. Africa contain organic particles which closely resemble, in detailed morphology, coacervates, proteinoid microspheres or fossil and recent microorganisms. The microphotospectrographs in visible and near-ultraviolet light of these minute particles revealed a strong absorption peak at the vicinity of 4000 A, which is indicative of lipids. Hydrothermal mineral from veins from a number of other localities proved to contain the biomorphic organic particles. The theoretical significance of these organic particles is discussed with reference to problems of origin of life.

Mueller, G.↗

The Willowbar meteorite

Results of microscopic and electron microprobe analyses of a meteorite which fell near Willowbar, Okla., in December 1971. Microscopic examination confirmed the differences in structure which could be seen on the cut faces. Three distinct regions could be recognized: unblackened, blackened, and black veins. Microprobe analyses of the silicate grains in the Willowbar meteorite show that it is an L chondrite. The lack of well-defined chondrules and the large size of the maskelynite grains suggests that it is a type 6 chondrite according to the classification of Van Schmus and Wood (1967). It also has large black veins of the same composition as the matrix, and thus it might best be described as a shock-brecciated L6 chondrite.

Lange, D. E.↗

Intrathoracic and venous pressure relationships during responses to changes in body position

Simultaneous end-expiratory pressures, referred to midthoracic level, in the superior and abdominal venae cavae, pericardial space, and right and left heart, were recorded without thoracotomy in three anesthetized dogs during sudden changes from supine to vertical head-up or head-down body positions. Intrathoracic and dependent great vein pressures referred to midchest level (sixth thoracic vertebra) decreased and showed simple hydrostatic gradients in either vertical position. However, a discontinuity in the large vein hydrostatic gradient occurred just distal to the superior margin of the thorax in either body position and was resumed again above this level. It is concluded that, just as the cerebrospinal fluid and intraperitoneal pressures minimize the effects of gravitational and inertial forces on the cerebral and visceral circulations, the pericardial and pleural pressures have a similar role for the heart proper.

Avasthey, P.↗

Regulator for intravenous feeding

Float valve maintains constant level of solution, providing constant drop rate as long as solution can flow into patient's vein. Second float valve allows solution to enter vein, but prevents entry of air.

Dimeff, J.↗

Anthropometric changes and fluid shifts

Several observations of body size, shape, posture, and configuration were made to document changes resulting from direct effects of weightlessness during the Skylab 4 mission. After the crewmen were placed in orbit, a number of anatomical and anthropometric changes occurred including a straightening of the thoracolumbar spine, a general decrease in truncal girth, and an increase in height. By the time of the earliest in-flight measurement on mission day 3, all crewmen had lost more than two liters of extravascular fluid from the calf and thigh. The puffy facies, the bird legs effect, the engorgement of upper body veins, and the reduced volume of lower body veins were all documented with photographs. Center-of-mass measurements confirmed a fluid shift cephalad. This shift remained throughout the mission until recovery, when a sharp reversal occurred; a major portion of the reversal was completed in a few hours. The anatomical changes are of considerable scientific interest and of import to the human factors design engineer, but the shifts of blood and extravascular fluid are of more consequence. It is hypothesized that the driving force for the fluid shift is the intrinsic and unopposed lower limb elasticity that forces venous blood and then other fluid cephalad.

Thornton, W. E.↗

Influence of G-forces and hypodynamia on the portal system of the liver

Longitudinally and transversely applied g-forces as intolerable levels cause damage to the interlobular veins of the liver, with maximum damage caused by dorso-ventral g-force application. The portal vein system is more resilient with respect to g-forces at the limit of tolerance.

Drozdova, A. V.↗

Mineralogy of lunar norite 78235 - Second lunar occurrence of P2 sub 1 ca pyroxene from Apollo 17 soils

A thin section of lunar rock 78235 is examined optically as well as with an electron microprobe and is shown to be a highly-shocked coarse norite. The rock is found to consist of approximately 30% low-Ca pyroxene, 55% Ca-rich plagioclase, and 15% glass veins. The composition of the glass veins is analyzed, and it is suggested that they resulted from shock melting of the norite. Comparisons with other noritic rocks indicate that the pyroxene in rock 78235 has P2 sub 1 ca symmetry. Based on this symmetry plus the coarse grain size, it is concluded that rock 78235 was formed under plutonic conditions. The origin of the minor phases in this rock is briefly considered.

Steele, I. M.↗

Effect of gravitational overloads, hypokinesia and hypodynamia on the vessels of the pulmonary blood circuit

Vessels of the pulmonary circuit are studied under normal conditions, in exposure to single stress or continuous threshold endurable chestspine gravitational stresses, and one to eight weak hypokinesia and hypodynamic effects followed by stress. Examination methods include rentgenography and microrentgenography, clearing, and histology. In exposure to gravitational stress the distal portions of the arterial vessels of the 3 and 4 orders constrict, while all veins dilate. Sinuosity of all vessels is noted. The volume of the capillary bed increases and signs of perivascular edema occur. Due to hypokinesia and hypodynamia the arteries constricted and the arterial bed becomes poor. The veins of all orders dilate and the volume of the capillary bed increases. The changes grew greater the longer the terms of hypodyamic effects. Successive combination of hypokinesia and hypodynamia and gravitational stresses cause more pronounced changes than separate effects of these two factors and result in great deformity of the vascular walls, including their rupture and penetration of formed elements beyond the limits of the vascular bed.

Kasimtsev, A. A.↗

The microstructure of minerals in coarse-grained Ca-Al-rich inclusions from the Allende meteorite

Transmission electron microscopy (TEM) is used to investigate the microstructure and microchemistry of minerals in Ca-Al-rich, coarse-grained inclusions (CAI) from the meteorite. The spinels contain only low to moderate dislocation densities and are characterized by a ubiquitous, fine black spotty texture that is thought to originate from a slightly nonstoichiometric composition. Whereas the Ti-Al-pyroxenes are relatively devoid of features, they contain veins of secondary phases apparently deposited in unhealed cracks. Chromite is identified in the veins, indicating transport of oxidized iron during alteration. The melilites show the greatest variety of microstructures and are the most heavily altered phase in CAI. High dislocation densities are common and the crystals exhibit considerable internal strain, suggesting that they have not been annealed.

Barber, D. J.↗

Mobile trace element contents in Jilin chondrite

A determination is conducted of ppm-ppt levels of Co, Se, Ga, Rb, Cs, Te, Bi, Ag, In, Tl, Zn and Cd (arranged empirically in order of increasing mobility at 1000 C) by radiochemical neutron activation analysis in eight Jilin samples of known Ar-40 content and a large vein from one of these. The trace element contents of the vein do not differ markedly from those of Jilin whole-rock samples. Only Rb correlates with Ar-40, possibly due to chance. All elements but Cd are present at levels similar to thdse in H4-6 chondrites with long K/Ar age (i.e., presumably mildly shocked). These levels are low relative to those in analogous L4-6 chondrites suggesting that H chondrites formed and/or evolved under higher pre-shock temperatures than did mildly shocked L chondrites. Time-temperature conditions during shock-loading of Jilin parent material were mild relative to those in strongly shocked L chondrites, being sufficient at most only to mobilize Cd.

Sakuragi, Y.↗

Sources of scattering in vegetarian and other surfaces and objects

The sources of scattering in vegetation and other surfaces and objects were studied. A special radar, SOURCESCAT, that could resolve a cylindrical volume 18 cm in diameter and 11 cm long was built. This system provided the first really fine-resolution measurements of radar backscatter from vegetation. The measurements showed that many of the assumptions used previously in modeling vegetation backscatter were false. Vegetation studied included various field crops, prairie grass, and various trees. Major differences were found in the roles of leaves, branches, stems, and trunks for different species. An artificial tree was studied in the laboratory using the systems. The most significant findings were that the average radar volume scattering coefficient is independent of azimuth, and that slanting of the polarization vector can give useful information not available with ordinary vertical and horizontal polarization. A model for scattering from a single leaf was developed. This model, for the first time, took into account the presence of veins in leaves. The pattern of scatter from a leaf was shown quite different from that for which the veins are ignored. A list of publications and presentations resulting from this project are attached.

Moore, R. K.↗

Ureilites - Trace element clues to their origin

The question of the origin of ureilites was reexamined using new data obtained by radiochemical NAA for Ag, Au, Bi, Br, Cd, Cs, Ge, In, Ir, Ni, Pd, Os, Rb, Re, Sb, Se, Te, Tl, U, and Zn in two vein separates from Haveroe and Kenna and a bulk sample of Kenna. Vein material was found to be enriched in all elements analyzed, except Zn, and to account for most of the carbon, noble gases, and, presumably, siderophiles in the meteorite. The results support the earlier interpretation of Higuchi et al. (1976) on the composition of ureilite parent body (similar to C3V or H3, but not C3O chondrites).

Janssens, Marie-Josee↗

Shock metamorphism of deformed quartz

The effect produced by shock loading (to peak pressures of 12 and 24) on deformed synthetic quartz containing a dislocation and abundant bubbles and small inclusions was investigated, and the relationships between preexisting dislocation density shock lamellae in the target material were examined. The resultant material was found to be inhomogeneously deformed and extremely fractured. Results of TEM examinations indicate that no change in dislocation density was caused by shock loading except in regions containing shock lamellae, where the dislocation density was lowered. The shock-induced defects tend to nucleate on and be controlled by preexisting stress concentrators; shock lamellae, glassy veins, and most curviplanar defects form in tension, presumably during release. An extremely mobile silica fluid is formed and injected into fractures during release, which forcibly removes crystalline fragments from vein walls. It is concluded that shock deformation in quartz is dominated by fracture and melting.

Gratz, Andrew J.↗

Aqueous alteration on the parent bodies of carbonaceous chondrites: Computer simulations of late-stage oxidation

CI carbonaceous chondrites may be products of hydrous alteration of CV- or anhydrous CM-type materials. The CIs typically contain veins filled with carbonates and sulfates, probably indicating a period of late stage aqueous alteration under oxidizing conditions. To test this idea, computer simulations of aqueous alteration of CV- and CM-type carbonaceous were performed. Simulations were restricted to the oxidation of hydrous mineral assemblages produced in previous simulations in order to determine whether further reaction and oxidation results in the phyllosilicate, carbonate, sulfate and oxide vein assemblages typical of CI carbonaceous chondrites. Our simulations were performed at 1, 25, 100, and 150 C (the appropriate temperature range) for the CV and CM mineral assemblages and using the computer code EQ3/6.

Bourcier, W. L.↗