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Presentation to support a Summit/TIM with various Stakeholders (Ground and Flight) to perform a GAP analysis associated with ground and spacecraft maintenance, maintainability, and availability activities in order to identify missing Agency planning and guidance materials.
Presentation to support a Summit/TIM with various Stakeholders (Ground and Flight) to perform a GAP analysis associated with ground and spacecraft maintenance, maintainability, and availability activities in order to identify missing Agency planning and guidance materials.
Vitamin B 12 (the cyanated form of cobalamin cofactors) is best known for its essential role in human health. In addition to its function in human metabolism, cobalamin also plays important roles in microbial metabolism and can impact microbial community function. Cobalamin is a member of the structurally diverse family of cofactors known as cobamides that are produced exclusively by certain prokaryotes. Cobamides are considered shared nutrients in microbial communities because the majority of bacteria that possess cobamide-dependent enzymes cannot synthesize cobamides de novo. Furthermore, different microbes have evolved metabolic specificity for particular cobamides, and therefore, the availability of cobamides in the environment is important for cobamide-dependent microbes. Determining the cobamides present in an environment of interest is essential for understanding microbial metabolic interactions. By examining the abundances of different cobamides in diverse environments, including 10 obtained in this study, we find that, contrary to its preeminence in human metabolism, cobalamin is relatively rare in many microbial habitats. Comparison of cobamide profiles of mammalian gastrointestinal samples and wood-feeding insects reveals that host-associated cobamide abundances vary and that fecal cobamide profiles differ from those of their host gastrointestinal tracts. Environmental cobamide profiles obtained from aquatic, soil, and contaminated groundwater samples reveal that the cobamide compositions of environmental samples are highly variable. As the only commercially available cobamide, cobalamin is routinely supplied during microbial culturing efforts. Furthermore, these findings suggest that cobamides specific to a given microbiome may yield greater insight into nutrient utilization and physiological processes that occur in these habitats.
The procedures adopted for analysis of photometric data in terms of color excesses, intrinsic color indexes, absolute visual magnitudes, and rotational-velocity effects are discussed in detail for Orion association B-, intermediate (I)-, and AF-type stars. The effects of the nebular environment and a comparison of various calibrations of Balmer-line and four-color indexes are considered for the determination of individual absolute magnitudes for B-type stars. When absolute magnitudes of stars in the region of the Orion Nebula are determined from the beta index, emission mechanisms appear to spuriously brighten them. A detailed comparison of absolute magnitudes derived from Balmer-line indexes and MK spectral-type calibrations is presented. The data are also examined with regard to the effects of polarization and infrared excesses. The results suggest a complex combination of intracluster and circumstellar origins for these processes.
Analysis of charring ablation with description of associated computing program
The California Energy Commission's Clean Transportation Program (CTP) supports a wide range of alternative, low-carbon fuel and vehicle projects. This report improves upon the 2014 Alternative and Renewable Fuel and Vehicle Technology Program (ARFVTP) Benefits Report(the former name of the Clean Transportation Program), which focused on two components of benefit calculation: expected benefits and market transformation benefits. The "expected benefits" are defined as benefits that accrue because of the direct displacement of petroleum-based fuels or vehicle technologies. The "market transformation benefits" accrue because of CTP funding shifting the underlying market dynamics and accelerating the adoption of alternative fuel vehicles. This report documents the updated methods used in the benefits analysis in 2014 and applies them for this 2021 Clean Transportation Program Benefits Report. The project team used data collected from CTP projects funded from 2009 to the third quarter of 2021 to estimate the benefits between 2021 and 2030. CTP projects valued at $\$898.3 million$ were assessed (out of $\$1.04 billion$ funded) to estimate expected benefits of 249 million gallons per year petroleum reduction and 2.79 million metric tons per year of carbon dioxide equivalent greenhouse gas (GHG) reduction in 2030. Market transformation benefits are additive to the expected benefits and were estimated with high and low ranges for the 315 relevant projects evaluated. The market transformation benefits' GHG reductions are estimated as 2.2 million to 6.2 million metric tons of carbon dioxide equivalent per year and the petroleum reductions as 145.3 million to 671.5 million gasoline gallon equivalents per year in 2030. Combining both benefit types, the CTP projects can make significant progress toward meeting California's long-term GHG and petroleum fuel use reduction goals.
In 2007, California Assembly Bills (AB) 118 and 109 created the Clean Transportation Program (CTP), which authorized the California Energy Commission (CEC) to fund projects concerning alternative fuels and advanced transportation technologies to help attain the state's climate change policies. AB 109 also required CEC to prepare a report summarizing the benefits resulting from the program. Since then, CEC has collaborated with National Renewable Energy Laboratory to prepare and publish biannual reports summarizing CTP's benefits related to petroleum displacement, greenhouse gas (GHG) reductions, air pollution, and more. This report updates the input data, calculation methodologies, and resulting outputs from CEC's 2021 report.
This repository contains the analysis code and supporting datasets associated with the study “Genomic signatures in Variovorax enabling colonization of the Populus endosphere.” Beals DG, Carper DL, Hochanadel LH, Jawdy SS, Klingeman DM, Piatkowski BT, Weston DJ, Doktycz MJ, Pelletier DA. 2026. Genomic signatures in Variovorax enabling colonization of the Populus endosphere. mSystems 11:e01605-25. https://doi.org/10.1128/msystems.01605-25 The scripts are organized sequentially (01–07) and document the workflows used for: Sequence-read alignment and feature counting Orthogroup and KEGG Ortholog annotation Count normalization Statistical analysis and aggregation Generation of manuscript figures and tables Repository contents The uncompressed files are the finalized, formatted datasets used to generate the figures and tables reported in the study, including the supplemental CSV files referenced in the manuscript. The accompanying ZIP archive contains the complete codebase and example data_input/ and data_output/ directories illustrating the organization and execution of the analytical workflow. Individual scripts identify the corresponding manuscript analyses and figure panels. Raw sequencing data Raw sequencing reads are available through the NCBI Sequence Read Archive under BioProject accession PRJNA1322484.
The effect that one candidate inlet, an assembly of capillary openings in a thin glass plate (a multichannel plate), might have on the overall sampling process is considered. The flow characteristics of the plate, under a variety of conditions of external pressure and mass flow, is evaluated. A review of capillary flow theory is presented with some development of the pertinent equations. The predicted mass flow will be compared to that determined perimentally to assess the effect that mass flow through one capillary might have upon a neighboring capillary. Mass spectrometric measurements of mixtures flowing through the multichannel plate (MCP) is also considered. In the first part of the experiments, the flow was in a direction normal to the surface of the plate. The experimental gases were Argon and mixtures of carbon dioxide in air. Ongoing experiments are discussed which are conducted with the flow parallel to the surface of the plate, a simulation of the kind of flow that a flight vehicle would experience.
In early 2003, the Mars Icy Sample Team (MIST) was formed to address several questions related to the acquisition and analysis of ice-bearing samples on the surface of Mars by a robotic mission. These questions were specifically framed in the context of planning for the 2009 Mars Science Laboratory (MSL) lander, but the answers will also also have value in planning other future landed investigations.
Planetary Protection (PP) requirements help to ensure the integrity of life-detection science conducted on solar system bodies, particularly those with liquid water. Such requirements are often met by a combination of cleaning and verification steps to reduce microbial contamination (i.e. bioburden) to an acceptable limit. There are only two cleaning methods approved by the National and Space Administration (NASA), vapor hydrogen peroxide (VHP) and heat microbial reduction (HMR), the latter of which is the most commonly employed. However, HMR can be harsh and produce its own concerns about material integrity, like aging or increasing hardness. When bioburden reduction methods cannot be employed, the project must rely on assumed NASA specification values of bioburden which can cause compliance issues with strict PP bioburden limitations. This work aimed to more accurately define the risk of contamination of a target body by providing empirical data associated with raw materials commonly used in solid rocket motors for lander missions. In this study, we pulverized nonmetallic materials using a cryogenic grinder, then analyzed the resulting substrate for microbial colony forming units (CFU). We found that many nonmetallic materials do not harbor large bioburden when compared with the NASA specification values, though a range existed depending on the material, treatment, and handling. The results from this work provide quantitative data to help reduce concerns of contamination, while also providing a foundation for follow up studies into additional sterilization methods and molecular identification of contaminating microbes.
Functional network analysis of 112 GWAS-identified candidate genes for yield-related traits in pennycress ( Thlaspi arvense ). Uses RWRtools Lines of Evidence to propagate Arabidopsis orthologs through a nine-layer multiplex functional network.
This document contains a basic analysis of the meteorology associated with the NASA Glenn Twin Otter icing encounters between December 1997 and March 1998. The purpose of this analysis is to provide a meteorological context for the aircraft data collected during these flights. For each case, the following data elements are presented: (1) A brief overview of the Twin Otter encounter, including locations, liquid water contents, temperatures and microphysical makeup of the clouds and precipitation aloft, (2) Upper-air charts, providing hand-analyzed locations of lows, troughs, ridges, saturated/unsaturated air, temperatures, warm/cold advection, and jet streams, (3) Balloon-borne soundings, providing vertical profiles of temperature, moisture and winds, (4) Infrared and visible satellite data, providing cloud locations and cloud top temperature, (5) 3-hourly surface charts, providing hand-analyzed locations of lows, highs, fronts, precipitation (including type) and cloud cover, (6) Hourly, regional radar mosaics, providing fine resolution of the locations of precipitation (including intensity and type), pilot reports of icing (including intensity and type), surface observations of precipitation type and Twin Otter tracks for a one hour window centered on the time of the radar data, and (7) Hourly plots of icing pilot reports, providing the icing intensity, icing type, icing altitudes and aircraft type. Outages occurred in nearly every dataset at some point. All relevant data that was available is presented here. All times are in UTC and all heights are in feet above mean sea level (MSL).
This part of the document contains an analysis of the meteorology associated with the premier icing encounters from the January-March 1997 NASA Twin Otter dataset. The purpose of this analysis is to provide a meteorological context for the aircraft data collected during these flights. For each case, the following data elements are presented: (1) A detailed discussion of the Twin Otter encounter, including locations, liquid water contents, temperatures and microphysical makeup of the clouds and precipitation aloft, (2) Upper-air charts, providing hand-analyzed locations of lows, troughs, ridges, saturated/unsaturated air, temperatures, warm/cold advection, and jet streams, (3) Balloon-borne soundings, providing vertical profiles of temperature, moisture and winds, (4) Infrared satellite data, providing cloud locations and cloud top temperature, (5) 3-hourly surface charts, providing hand-analyzed locations of lows, highs, fronts, precipitation (including type) and cloud cover, (6) Hourly plots of icing pilot reports, providing the icing intensity, icing type, icing altitudes and aircraft type, (7) Hourly, regional radar mosaics, providing fine resolution of the locations of precipitation (including intensity and type), pilot reports of icing (including intensity and type), surface observations of precipitation type and Twin Otter tracks for a one hour window centered on the time of the radar data, and (8) Plots of data from individual NEXRAD radars at times and elevation angles that have been matched to Twin Otter flight locations. Outages occurred in nearly every dataset at some point. All relevant data that was available is presented here. All times are in UTC and all heights are in feet above mean sea level (MSL).
The details of an integrated general-purpose finite element structural analysis computer program which is also capable of solving complex multidisciplinary problems is presented. Thus, the SOLIDS module of the program possesses an extensive finite element library suitable for modeling most practical problems and is capable of solving statics, vibration, buckling, and dynamic response problems of complex structures, including spinning ones. The aerodynamic module, AERO, enables computation of unsteady aerodynamic forces for both subsonic and supersonic flow for subsequent flutter and divergence analysis of the structure. The associated aeroservoelastic analysis module, ASE, effects aero-structural-control stability analysis yielding frequency responses as well as damping characteristics of the structure. The program is written in standard FORTRAN to run on a wide variety of computers. Extensive graphics, preprocessing, and postprocessing routines are also available pertaining to a number of terminals.
A discussion of cosmic dust, its classification, the usefulness of its analysis, and problems associated with its analysis is presented. All measurements on stratospheric dust particles presented here were made with secondary ion mass spectrometry (SIMS). The SIMS technique turns out to be an extremely useful tool in the study of very small particles and made a number of firsts possible. The isotopic composition of cosmic dust particles were measured for the first time. It was also the first time that it was possible to measure the abundances of the rare earth elements in these particles. However, the most important advance this work represents is that both trace elemental and isotopic abundances were successfully measured in the same particles. Thus, for the first time, it is possible to compare isotopic and trace element abundance data of the same cosmic dust particles.
The steady-state, three-dimensional transport equation for inert pollutant dispersion in the atmosphere is solved analytically to obtain expressions for pollutant concentrations from both a point source and a rectangular area source. The results of this analysis have application to numerical grid models in which the smallest resolvable source is the size of a single grid. The concentrations at ground level along the plume centerline from the two types of sources are in close agreement with each other at distances greater than 10 km downwind when the horizontal dimension of the rectangular source is not less than 100 m. The dimension of the source in the vertical direction is held constant at 50 m. Similar results are obtained for concentrations along the plume centerline at the effective stack height. When the size of the source in the crosswind direction is increased to 1 km, good agreement between the two concentrations is obtained for downwind distances over 60 km for the ground level case and over 140 km for the concentration at stack height level. For the horizontal dimension equal to 10 km, good agreement between the two cases is only obtained at extremely large downwind distances at both the stack height and ground levels.