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At least 109 records · Page 6

Renewable energy and biological conservation in a changing world

Without a shift to renewable energy sources, climate change will have adverse effects on many terrestrial and aquatic species. On the other hand, replacing fossil fuels with renewable energy will have different effects on wildlife, some negative. To protect biota, while navigating the complexities surrounding the transition to renewable energy, will require sharing knowledge between energy experts and wildlife experts, as well as policy makers and resource managers. This special issue is intended to advance the conversation. Interactions between renewable energy facilities and fauna are complex. Each source of renewable energy has its own conservation concerns, and each presents its own set of opportunities for mitigation; Other concerns and mitigations are shared. In this overview, we review climate risks to biota, clarify the complementarities among renewables for the conservation community, and summarize research on energy effects on biota for the energy community. The papers invited for this special issue address fossil (coal, oil, natural gas) and renewable energy (terrestrial biomass energy, hydropower, marine-hydrokinetic energy, wind energy, solar energy) and affected fauna. Finally, we hope that the results of these studies, and others like them, set us on a path toward low-carbon energy systems that provide society with energy while protecting future biodiversity.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Immediate impacts of soybean cover crop on bacterial community composition and diversity in soil under long-term Saccharum monoculture

Saccharum yield decline results from long-term monoculture practices. Changes in cropping management can improve soil health and productivity. Below-ground bacterial community diversity and composition across soybean (Glycine max (L.) Merr) cover crop, Saccharum monoculture (30+ year) and fallowed soil were determined. Near full length (~1,400 base pairs) of 16S rRNA gene sequences were extracted from the rhizospheres of sugarcane and soybean and fallowed soil were compared. Higher soil bacterial diversity was observed in the soybean cover crop than sugarcane monoculture across all measured indices (observed operationational taxonomic units, Chao1, Shannon, reciprocal Simpson and Jackknife). Acidocateria, Proteobacteria, Bacteroidetes and Planctomycetes were the most abundant bacterial phyla across the treatments. Indicator species analysis identified nine indicator phyla. Planctomycetes, Armatimonadetes and candidate phylum FBP were associated with soybean; Proteobacteria and Firmicutes were linked with sugarcane and Gemmatimonadetes, Nitrospirae, Rokubacteria and unclassified bacteria were associated with fallowed soil. Non-metric multidimensional scaling analysis showed distinct groupings of bacterial operational taxonomic units (97% identity) according to management system (soybean, sugarcane or fallow) indicating compositional differences among treatments. This is confirmed by the results of the multi-response permutation procedures (A = 0.541, p = 0.00045716). No correlation between soil parameters and bacterial community structure was observed according to Mantel test (r = 211865, p = 0.14). Use of soybean cover-crop fostered bacterial diversity and altered community structure. This indicates cover crops could have a restorative effect and potentially promote sustainability in long-term Saccharum production systems.

Bacterial diversity↗

Environmental DNA Detection in Marine Macrophyte Ecosystems as a Potential Blue Carbon Source in Sediments

“Blue carbon” refers to the carbon sequestered by the world’s oceanic and coastal ecosystems, particularly through coastal vegetation such as mangroves, salt marshes, seagrasses, and marine macroalgae. These ecosystems play a crucial role in the global carbon cycle by serving as significant carbon sinks, absorbing carbon dioxide from the atmosphere and storing it in biomass and sediments over long periods. This study explores the use of environmental DNA (eDNA) to detect marine macrophytes and microalgae assemblages contributing to blue carbon in sediments across various coastal ecosystems. The research addresses the challenges of traditional monitoring methods by utilizing high-throughput sequencing of the 18S-V9 region amplified using eDNA from sediment samples collected at eight locations in the United States and South Korea. The results reveal a diverse array of taxa, underscoring the variability in community composition across different conditions. Notably, sites with seagrass beds and Ulva blooms showed distinct patterns in microalgal community structure. This study underscores the potential of eDNA analysis in providing comprehensive insights into the biodiversity of marine macrophyte ecosystems, thus informing conservation efforts and enhancing the understanding of marine ecological dynamics.

Xing, Qikun (ORCID:0000000206179027)↗

One hundred priority questions for advancing seagrass conservation in Europe

Societal Impact Statement Seagrass ecosystems are of fundamental importance to our planet and wellbeing. Seagrasses are marine flowering plants, which engineer ecosystems that provide a multitude of ecosystem services, for example, blue foods and carbon sequestration. Seagrass ecosystems have largely been degraded across much of their global range. There is now increasing interest in the conservation and restoration of these systems, particularly in the context of the climate emergency and the biodiversity crisis. The collation of 100 questions from experts across Europe could, if answered, improve our ability to conserve and restore these systems by facilitating a fundamental shift in the success of such work. Summary Seagrass meadows provide numerous ecosystem services including biodiversity, coastal protection, and carbon sequestration. In Europe, seagrasses can be found in shallow sheltered waters along coastlines, in estuaries & lagoons, and around islands, but their distribution has declined. Factors such as poor water quality, coastal modification, mechanical damage, overfishing, land‐sea interactions, climate change and disease have reduced the coverage of Europe’s seagrasses necessitating their recovery. Research, monitoring and conservation efforts on seagrass ecosystems in Europe are mostly uncoordinated and biased towards certain species and regions, resulting in inadequate delivery of critical information for their management. Here, we aim to identify the 100 priority questions, that if addressed would strongly advance seagrass monitoring, research and conservation in Europe. Using a Delphi method, researchers, practitioners, and policymakers with seagrass experience from across Europe and with diverse seagrass expertise participated in the process that involved the formulation of research questions, a voting process and an online workshop to identify the final list of the 100 questions. The final list of questions covers areas across nine themes: Biodiversity & Ecology; Ecosystem services; Blue carbon; Fishery support; Drivers, Threats, Resilience & Response; Monitoring & Assessment; Conservation & Restoration; Governance, Policy & Management; and Communication. Answering these questions will fill current knowledge gaps and place European seagrass onto a positive trajectory of recovery.

Nordlund, Lina Mtwana↗

Identifying management opportunities to combat climate, land, and marine threats across less climate exposed coral reefs

Conserving coral reefs is critical for maintaining marine biodiversity, protecting coastlines, and supporting livelihoods in many coastal communities. Climate change threatens coral reefs globally, but researchers have identified a portfolio of coral reefs (bioclimatic units [BCUs]) that are relatively less exposed to climate impacts and strongly connected to other coral reef systems. These reefs provide a proactive opportunity to secure a long-term future for coral reefs under climate change. To help guide local management efforts, we quantified marine cumulative human impact (CHI) from climate, marine, and land pressures (2013 and from 2008 to 2013) in BCUs and across countries tasked with BCU management. Additionally, we created a management index based on common management measures and policies for each pressure source (climate, marine, and land) to identify a country's intent and commitment to effectively manage these pressures. Twenty-two countries (79%) had increases in CHI from 2008 to 2013. Climate change pressures had the highest proportional contribution to CHI across all reefs and in all but one country (Singapore), but 18 BCUs (35%) and nine countries containing BCUs (32%) had relatively high land and marine impacts. There was a significant positive relationship between climate impact and the climate management index across countries (R 2 = 0.43, p = 0.02), potentially signifying that countries with greater climate impacts are more committed to managing them. However, this trend was driven by climate management intent in Fiji and Bangladesh. Our results can be used to guide future fine-scale analyses, national policies, and local management decisions, and our management indices reveal areas where management components can be improved. Cost-effectively managing local pressures (e.g., fishing and nutrients) in BCUs is essential for building a climate-ready future that benefits coral reefs and people.

59 BASIC BIOLOGICAL SCIENCES↗

Evaluating the potential for bird‐habitat models to support biodiversity‐friendly urban planning

Abstract Urban expansion poses a major threat to wildlife populations. Biodiversity‐friendly urban landscapes could deliver benefits for both wildlife and people, by incorporating conservation and ecosystem services objectives. Well‐designed urban developments could also soften the ecological impacts of urbanization. However, delivering urban landscapes that integrate biodiversity requirements effectively remains challenging. Ecological models, designed to predict wildlife population responses to alternative urban designs, could prove effective in supporting the creation of biodiversity‐friendly urban landscapes. Here, we combine national‐scale bird abundance data with high resolution, spatially explicit habitat data to characterize relationships between bird densities and urban landscape form in Britain. From these analyses and cross validation, we evaluate the potential for well‐parameterized, species‐specific models to be used to predict bird densities in novel or modified urban areas. Our analyses indicate that responses of bird abundance to urban habitat are species‐specific and complex, with few variables consistently affecting a large proportion of species. However, contiguous areas of greenspace within urban sites are preferential for accommodating breeding birds, compared to a more fragmented arrangement of multiple, small greenspace patches. In combination, the bird‐habitat relationships identified could successfully predict observed variation in abundance for most bird species considered. Further evaluation of habitat descriptor variables, spatial scales of species' habitat use and analytical modelling approaches may be needed to improve the predictive ability of bird‐habitat models for certain species, particularly waterbirds and those observed less frequently in urban areas. Synthesis and applications . We modelled breeding bird abundance in built‐up areas with respect to the characteristics and contexts of urban environments. While most variables were important for multiple species, responses overall were species‐specific, so simple assemblage metrics, like diversity, will not describe the variation in bird communities well. However, the results illustrate the potential of an evidence‐based, spatially explicit evaluation of urban development impacts on biodiversity, by predicting the consequences for bird numbers. Subject to verification of predictive ability, practitioners can apply the models to compare, for example, land‐sparing and sharing within developments, or to quantify the biodiversity requirements for effective offsetting. This would be facilitated by incorporation into an online tool allowing user‐determined input scenarios.

Plummer, Kate E.↗

Habitat severity characteristics structure soil communities at regional and local spatial scales along the Antarctica Peninsula

Antarctic soils provide an excellent setting to test biogeographical patterns across spatial and environmental scales given their relatively simple communities and the dominance of physical factors that create strong environmental gradients. Additional urgency is given by the fact that their unique terrestrial communities are the subject of conservation efforts in a rapidly changing environment. We investigated relationships of soil community assembly and alpha and beta diversity with climatic and environmental parameters across regional and local scales in Maritime Antarctica. We sampled from a regional gradient of sites that differ in habitat severity, ranging from relatively favourable to harsher physicochemical conditions. At the regional scale, bacterial community characteristics and microarthropod abundance varied along this severity gradient, but most measures of fungal communities did not. Microarthropod and microbial communities differed in which soil and climate parameters were most influential, and the specific parameters that influenced each taxon differed across broad and fine spatial scales. This suggests that conservation efforts will need to focus on a large variety of habitat characteristics to successfully encompass diversity across taxa. Because beta diversity was the result of species turnover, conservation efforts also cannot focus on only the most biodiverse sites to effectively preserve all aspects of biodiversity.

54 ENVIRONMENTAL SCIENCES↗

The erosion of biodiversity and biomass in the Atlantic Forest biodiversity hotspot

Tropical forests are being deforested worldwide, and the remaining fragments are suffering from biomass and biodiversity erosion. Quantifying this erosion is challenging because ground data on tropical biodiversity and biomass are often sparse. Here, we use an unprecedented dataset of 1819 field surveys covering the entire Atlantic Forest biodiversity hotspot. We show that 83-85% of the surveys presented losses in forest biomass and tree species richness, functional traits, and conservation value. On average, forest fragments have 25-32% less biomass, 23-31% fewer species, and 33, 36, and 42% fewer individuals of late-successional, large-seeded, and endemic species, respectively. Biodiversity and biomass erosion are lower inside strictly protected conservation units, particularly in large ones. We estimate that biomass erosion across the Atlantic Forest remnants is equivalent to the loss of 55-70 thousand km 2 of forests or US$2.3-2.6 billion in carbon credits. These figures have direct implications on mechanisms of climate change mitigation.

54 ENVIRONMENTAL SCIENCES↗

Enumerating soil biodiversity

Soil is an immense habitat for diverse organisms across the tree of life, but just how many organisms live in soil is surprisingly unknown. Previous efforts to enumerate soil biodiversity consider only certain types of organisms (e.g., animals) or report values for diverse groups without partitioning species that live in soil versus other habitats. Here, we reviewed the biodiversity literature to show that soil is likely home to 59 ± 15% of the species on Earth. We therefore estimate an approximately two times greater soil biodiversity than previous estimates, and we include representatives from the simplest (microbial) to most complex (mammals) organisms. Enchytraeidae have the greatest percentage of species in soil (98.6%), followed by fungi (90%), Plantae (85.5%), and Isoptera (84.2%). Our results demonstrate that soil is the most biodiverse singular habitat. By using this estimate of soil biodiversity, we can more accurately and quantitatively advocate for soil organismal conservation and restoration as a central goal of the Anthropocene.

Science & Technology - Other Topics↗

Role of sustainable biomass markets in forest conservation

When properly implemented, the production of bioenergy using wood pellets can play a role in addressing threats related to biodiversity and climate change. Reduction in forest loss and degradation is fostered by the integration of sustainable pellet production with other forest markets and strategies to implement nature-based solutions. Local stakeholders should be engaged to devise management practices appropriate for the location and context. Market incentives for sustainable biomass production help conserve forests.

09 BIOMASS FUELS↗

A heuristic tool to assess regional impacts of renewable energy infrastructure on conservation areas

Wind and hydropower are important renewable components of national energy portfolios, but their infrastructure negatively affects biodiversity. Regional development requires identification of scenarios that minimize the cumulative impacts of multiple facilities. We introduce the cumulative impact plot (CIP) to quantify cumulative impacts of renewable energy development within a region. Summed impacts of facilities are plotted as a function of the number of facilities, with facilities ranked by increasing (the best-case scenario) or decreasing (worst-case scenario) individual impact. These curves represent lower and upper bounds to which alternative development scenarios (e.g., facilities ranked by generating capacity) can be compared. We used CIPs to assess overlap of potential wind and hydropower facilities with two types of conservation area in the United States: (1) federally protected lands and (2) critical habitats of federally threatened and endangered species. Here, we evaluated two alternative scenarios: facilities ranked by (A) decreasing generating capacity and (B) increasing distance from urban centers. Differences between the best-case and worst-case scenarios were large, thus revealing opportunities to develop facilities with limited impact on conservation areas. Alternative scenarios maximizing energy density generally resulted in conservation area overlap intermediate to best-case and worst-case scenarios. CIPs can also identify the proportion of wind versus hydropower favored under alternative scenarios. Build-out scenarios aimed at minimizing conservation area overlap favor hydropower, whereas alternative scenarios favor wind power. We conclude that CIPs can (1) complement—but should not replace—project-level environmental impact assessment, (2) integrate strategic, cumulative, and scenario-based assessments, and (3) harness rapidly growing geospatial data.

54 ENVIRONMENTAL SCIENCES↗

Harvest Intensity Effects on Carbon Stocks and Biodiversity Are Dependent on Regional Climate in Douglas-Fir Forests of British Columbia

Temperate forests provide crucial ecosystems services as living sinks for atmospheric carbon (C) and repositories of biodiversity. Applying harvesting at intensities that minimize losses offers one means for mitigating global change. However, little is known of overstory retention levels that best conserve ecosystem services in different regional climates, and likewise as climate changes. To quantify the effect of harvest intensity on C stocks and biodiversity, we compared five harvesting intensities (clearcutting, seedtree retention, 30% patch retention, 60% patch retention, and uncut controls) across a climatic aridity gradient that ranged from humid to semi-arid in the Douglas-fir (Pseudotsuga menziesii) forests of British Columbia. We found that increased harvesting intensity reduced total ecosystem, above ground, and live tree C stocks 1 year post-harvest, and the magnitude of these losses were negatively correlated with climatic aridity. In humid forests, total ecosystem C ranged from 50% loss following clearcut harvest, to 30% loss following large patch retention harvest. In arid forests this range was 60 to 8% loss, respectively. Where lower retention harvests are sought, the small patch retention treatment protected both C stocks and biodiversity in the arid forests, whereas the seedtree method performed as well or better in the humid forests. Below ground C stocks declined by an average of 29% after harvesting, with almost all of the loss from the forest floor and none from the mineral soil. Of the secondary pools, standing and coarse deadwood declined in all harvesting treatments regardless of cutting intensity or aridity, while C stocks in fine fuels and stumps increased. The understory plant C pool declined across all harvesting intensities in the humid forests, but increased in arid forests. Shannon’s diversity and richness of tree and bryoid species declined with harvesting intensity, where tree species losses were greatest in the humid forests and bryoid losses greatest in arid forests. Shrub and herb species were unaffected. This study showed that the highest retention level was best at reducing losses in C stocks and biodiversity, and clearcutting the poorest, and while partial retention of canopy trees can reduce losses in these ecosystem services, outcomes will vary with climatic aridity.

54 ENVIRONMENTAL SCIENCES↗

Classification of animal sounds in a hyperdiverse rainforest using convolutional neural networks with data augmentation

To protect tropical forest biodiversity, we need to be able to detect it reliably, cheaply, and at scale. Automated detection of sound producing animals from passively recorded soundscapes via machine-learning approaches is a promising technique towards this goal, but it is constrained by the necessity of large training data sets. Using soundscapes from a tropical forest in Borneo and a Convolutional Neural Network model (CNN), we investigate i) the minimum viable training data set size for accurate prediction of call types (‘sonotypes’), and ii) the extent to which data augmentation and transfer learning can overcome the issue of small and imbalanced training data sets. We found that even relatively high sample sizes (>80 per sonotype) lead to mediocre accuracy, which however improved significantly with data augmentation and transfer learning, including at extremely small sample sizes (3 per sonotype), regardless of taxonomic group or call characteristics. Neither transfer learning nor data augmentation alone achieved high accuracy. Our results suggest that transfer learning and data augmentation could make the use of CNNs to classify species’ vocalizations feasible even for small soundscape-based projects with many rare species. Retraining our open-source model requires only basic programming skills which makes it possible for individual conservation initiatives to match their local context, in order to enable more evidence-informed management of biodiversity.

54 ENVIRONMENTAL SCIENCES↗

Transcriptomic and functional analyses uncover a conserved effector driving genotype-dependent virulence in the Sphaerulina musiva-Populus trichocarpa interaction

The introduction of invasive microbes compromises the structure, biodiversity, and function of naïve ecosystems. Sphaerulina musiva, a hemibiotrophic pathogen that causes leaf spot and stem cankers in Populus species, exemplifies an invasive fungal pathogen spread by human activities. However, the genetic mechanisms of pathogenicity and virulence are poorly understood, impeding mitigation strategies. We utilized RNA sequencing to identify fungal effectors linked to stem canker formation, informing the development of future strategies for effective disease management. Our analysis revealed 70 genes differentially expressed at 2 weeks and 110 genes at 3 weeks between inoculated trees and controls. Notably, the gene with the highest expression at 2 weeks and the second highest at 3 weeks was homologous to Extracellular protein 2 (Ecp2). Complementary genome-wide association studies linked sequence polymorphisms in this locus to phenotypic variation in disease severity. Infiltration of S. musiva Ecp2 into Populus trichocarpa leaves induced necrosis in susceptible genotypes. Gene disruption using a CRISPR-Cas9 RNP system resulted in a genotype-dependent reduction of stem canker and disease severity. Tracing the evolutionary history of this effector across the fungal kingdom, we uncovered clade-specific gene-family expansions and orthologs in new species. These findings raise questions about the function and adaptive significance of these gene families in fungal lifestyles. Our study provides the first tractable target for breeding resistant poplar genotypes, addressing the challenges of managing S. musiva and uncovering mechanisms that drive its virulence, and provides deeper insights into the evolutionary dynamics of a conserved small-secreted protein with a diversity of functions.

Sondreli, Kelsey L [Oregon State University]↗

Temporal Resource Continuity Increases Predator Abundance in a Metapopulation Model: Insights for Conservation and Biocontrol

The amount of habitat in a landscape is an important metric for evaluating the effects of land cover on biodiversity, yet it fails to capture complex temporal dimensions of resource availability that could be consequential for species population dynamics. Here, we use a spatially-explicit predator–prey metapopulation model to test the effect of different spatiotemporal resource patterns on insect predators and their prey. We examined population responses in model landscapes that varied in both the amount and temporal variability of basal vegetation. Further, we examined cases where prey comprised either a single generalist species or two specialist species that use different resources available either early or late in the growing season. We found that predators and generalist prey benefitted from lower temporal variance of basal resources, which increased landscape-scale abundances. However, increasing the amount of basal resources also increased the variability of generalist prey populations. Specialist prey, on the other hand, did not benefit from less temporally variable basal resources, as they were restricted by habitat type, while also suffering greater predation. Predators achieved greater prey suppression in landscapes with less temporally variable resources, but the overall effects on prey abundance depended on prey habitat specialization. Our simulations demonstrate the joint importance of both the amount and temporal variability of resources for understanding how landscape heterogeneity influences biodiversity and ecosystem services such as the biological control of agricultural pests.

Spiesman, Brian↗

Integrated global assessment of the natural forest carbon potential

Forests are a substantial terrestrial carbon sink, but anthropogenic changes in land use and climate have considerably reduced the scale of this system. Remote-sensing estimates to quantify carbon losses from global forests are characterized by considerable uncertainty and we lack a comprehensive ground-sourced evaluation to benchmark these estimates. Here we combine several ground-sourced and satellite-derived approaches to evaluate the scale of the global forest carbon potential outside agricultural and urban lands. Despite regional variation, the predictions demonstrated remarkable consistency at a global scale, with only a 12% difference between the ground-sourced and satellite-derived estimates. At present, global forest carbon storage is markedly under the natural potential, with a total deficit of 226 Gt (model range = 151–363 Gt) in areas with low human footprint. Most (61%, 139 Gt C) of this potential is in areas with existing forests, in which ecosystem protection can allow forests to recover to maturity. The remaining 39% (87 Gt C) of potential lies in regions in which forests have been removed or fragmented. Although forests cannot be a substitute for emissions reductions, our results support the idea that the conservation, restoration and sustainable management of diverse forests offer valuable contributions to meeting global climate and biodiversity targets.

54 ENVIRONMENTAL SCIENCES↗

Rooting for function: community‐level fine‐root traits relate to many ecosystem functions

Humans are driving biodiversity change, which also alters community functional traits. However, how changes in the functional traits of the community alter ecosystem functions—especially belowground—remains an important gap in our understanding of the consequences of biodiversity change. We test hypotheses for how the root traits of the root economics space (composed of the collaboration and conservation gradients) are associated with proxies for ecosystem functioning across grassland and forest ecosystems in both observational and experimental datasets from 810 plant communities. First, we assessed whether community-weighted means of the root economics space traits adhered to the same trade-offs as species-level root traits. Then, we examined the relationships between community-weighted mean root traits and aboveground biomass production, root standing biomass, soil fauna biomass, soil microbial biomass, decomposition of standard and plot-specific material, ammonification, nitrification, phosphatase activity, and drought resistance. We found evidence for a community collaboration gradient but not for a community conservation gradient. Yet, links between community root traits and ecosystem functions were more common than we expected, especially for aboveground biomass, microbial biomass, and decomposition. These findings suggest that changes in species composition, which alter root trait means, will in turn affect critical ecosystem functions.

54 ENVIRONMENTAL SCIENCES↗

Summer Bat Monitoring Report for Calendar Year 2023

Bats are mammals belonging to the order Chiroptera, which means “hand-wing.” They use a thin membrane of skin stretched between their fingers to fly. All bat species known in the state of Washington are insectivorous and each bat can consume 600-1,000 insects per hour while feeding. Bats typically give birth once per year to a single pup, making bat populations vulnerable to impacts and slow to recover. Several species of bats have been documented on the Hanford Site, with nine species identified during The Nature Conservancy surveys in 1997 and 1998, and an additional seven species listed as potentially present as documented in Biodiversity Inventory and Analysis of the Hanford Site. Final Report 1994-1999 (Soll et al., 1999). Ongoing U.S. Department of Energy (DOE), Richland Operations Office (RL) acoustic surveys, including HNF-53759, Summer Bat Monitoring Report for Calendar Year 2012, have also confirmed the presence of the same nine species documented by Soll et al. (1999). An additional species, the spotted bat (Euderma maculatum), was observed roosting on the exterior of a facility by Hanford Site personnel in 2021; the species was confirmed by Hanford Site biologists via photograph (Table 1-1).

54 ENVIRONMENTAL SCIENCES↗