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At least 109 records · Page 6

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DOE OSTI2014

Analysis of clock-regulated genes in Neurospora reveals widespread posttranscriptional control of metabolic potential

Significance Circadian clocks regulate gene expression levels to allow an organism to anticipate environmental conditions. These clocks reside in all the major branches of life and confer a competitive advantage to the organisms that maintain them. The clock in the fungus Neurospora crassa is an excellent model for basic understanding of core circadian architecture as well as for filamentous fungi. Here, we identify genes whose expression is clock regulated; indeed, as much as 40% of the transcriptome may be clock regulated, broadly directing daytime catabolism and nighttime growth. Both transcriptional control and posttranscriptional regulation play major roles in control of cycling transcripts such that DNA binding of transcription factors alone appears insufficient to set the phase of circadian transcription.

Hurley, Jennifer M.↗

DOE OSTI2016

Quantum primordial standard clocks

In this paper, we point out and study a generic type of signals existing in the primordial universe models, which can be used to model-independently distinguish the inflation scenario from alternatives. These signals are generated by massive fields that function as standard clocks. The role of massive fields as standard clocks has been realized in previous works. Although the existence of such massive fields is generic, the previous realizations require sharp features to classically excite the oscillations of the massive clock fields. Here, we point out that the quantum fluctuations of massive fields can actually serve the same purpose as the standard clocks. We show that they are also able to directly record the defining property of the scenario type, namely, the scale factor of the primordial universe as a function of time a(t), but through shape-dependent oscillatory features in non-Gaussianities. Since quantum fluctuating massive fields exist in any realistic primordial universe models, these quantum primordial standard clock signals are present in any inflation models, and should exist quite generally in alternative-to-inflation scenarios as well. However, the amplitude of such signals is very model-dependent.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2025

Collinear Three-Photon Excitation of a Strongly Forbidden Optical Clock Transition

The 1 𝑆 0 − 3 𝑃 0 clock transition in strontium serves as the foundation for the world’s best atomic clocks and for gravitational wave detector concepts in clock atom interferometry. This transition is weakly allowed in the fermionic isotope 87 Sr but strongly forbidden in bosonic isotopes. Here, we demonstrate coherent excitation of the clock transition in bosonic 88 Sr using a novel collinear three-photon process in a weak magnetic field. We observe Rabi oscillations with frequencies of up to 50 kHz using W/cm 2 laser intensities and Gauss-level magnetic field amplitudes. The absence of nuclear spin in bosonic isotopes offers decreased sensitivity to magnetic fields and optical lattice light shifts, enabling atomic clocks with reduced systematic errors. The collinear propagation of the laser fields permits the interrogation of spatially separated atomic ensembles with common laser pulses, a key requirement for dark matter searches and gravitational wave detection with next-generation quantum sensors.

74 ATOMIC AND MOLECULAR PHYSICS↗

DOE OSTI2021

Breast-Specific Molecular Clocks Comprised of ELF5 Expression and Promoter Methylation Identify Individuals Susceptible to Cancer Initiation

A robust breast cancer prevention strategy requires risk assessment biomarkers for early detection. We show that expression of ELF5, a transcription factor critical for normal mammary development, is downregulated in mammary luminal epithelia with age. DNA methylation of the ELF5 promoter is negatively correlated with expression in an age-dependent manner. Both ELF5 methylation and gene expression were used to build biological clocks to estimate chronological ages of mammary epithelia. ELF5 clock-based estimates of biological age in luminal epithelia from average-risk women were within three years of chronological age. Biological ages of breast epithelia from BRCA1 or BRCA2 mutation carriers, who were high risk for developing breast cancer, suggested they were accelerated by two decades relative to chronological age. The ELF5 DNA methylation clock had better performance at predicting biological age in luminal epithelial cells as compared with two other epigenetic clocks based on whole tissues. We propose that the changes in ELF5 expression or ELF5-proximal DNA methylation in luminal epithelia are emergent properties of at-risk breast tissue and constitute breast-specific biological clocks.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2014

Daily Changes in Temperature, Not the Circadian Clock, Regulate Growth Rate in Brachypodium distachyon

Plant growth is commonly regulated by external cues such as light, temperature, water availability, and internal cues generated by the circadian clock. Changes in the rate of growth within the course of a day have been observed in the leaves, stems, and roots of numerous species. However, the relative impact of the circadian clock on the growth of grasses has not been thoroughly characterized. We examined the influence of diurnal temperature and light changes, and that of the circadian clock on leaf length growth patterns in Brachypodium distachyon using high-resolution time-lapse imaging. Pronounced changes in growth rate were observed under combined photocyles and thermocycles or with thermocycles alone. A considerably more rapid growth rate was observed at 28°C than 12°C, irrespective of the presence or absence of light. In spite of clear circadian clock regulated gene expression, plants exhibited no change in growth rate under conditions of constant light and temperature, and little or no effect under photocycles alone. Therefore, temperature appears to be the primary cue influencing observed oscillations in growth rate and not the circadian clock or photoreceptor activity. Furthermore, the size of the leaf meristem and final cell length did not change in response to changes in temperature. Therefore, the nearly five-fold difference in growth rate observed across thermocycles can be attributed to proportionate changes in the rate of cell division and expansion. A better understanding of the growth cues in B. distachyon will further our ability to model metabolism and biomass accumulation in grasses.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2015

Design and Evaluation of a Clock Multiplexing Circuit for the SSRL Booster Accelerator Timing System (Final Paper)

SPEAR3 is a 234 m circular storage ring at SLAC’s synchrotron radiation facility (SSRL) in which a 3 GeV electron beam is stored for user access. Typically the electron beam decays with a time constant of approximately 10hr due to electron lose. In order to replenish the lost electrons, a booster synchrotron is used to accelerate fresh electrons up to 3GeV for injection into SPEAR3. In order to maintain a constant electron beam current of 500mA, the injection process occurs at 5 minute intervals. At these times the booster synchrotron accelerates electrons for injection at a 10Hz rate. A 10Hz 'injection ready' clock pulse train is generated when the booster synchrotron is operating. Between injection intervals-where the booster is not running and hence the 10 Hz ‘injection ready’ signal is not present-a 10Hz clock is derived from the power line supplied by Pacific Gas and Electric (PG&E) to keep track of the injection timing. For this project I constructed a multiplexing circuit to 'switch' between the booster synchrotron 'injection ready' clock signal and PG&E based clock signal. The circuit uses digital IC components and is capable of making glitch-free transitions between the two clocks. This report details construction of a prototype multiplexing circuit including test results and suggests improvement opportunities for the final design.

43 PARTICLE ACCELERATORS↗

DOE OSTI2015

Design and Evaluation of a Clock Multiplexing Circuit for the SSRL Booster Accelerator Timing System - Oral Presentation [Slides]

43 PARTICLE ACCELERATORS↗

NASA NTRS1974

Relativistic effects of the rotation of the earth on remote clock synchronization

A treatment is given of relativistic clock synchronization effects due to the rotation of the earth. Unlike other approaches, the point of view of an earth fixed coordinate system is used which offers insight to many problems. An attempt is made to give the reader an intuitive grasp of the subject as well as to provide formulae for his use. Specific applications to global timekeeping, navigation, VLBI, relativistic clock experiments, and satellite clock synchronization are discussed. The question of whether atomic clocks are ideal clocks is also treated.

Reinhardt, V.↗

NASA NTRS1976

Comparison of bit synchronization schemes using AM and summed clocks

This paper considers bit synchronization through the use of a separate clock signal which is either amplitude modulated onto or summed with the data signal. For continuous data transmission, such schemes are known to be inferior, in the sense of efficient use of power, to schemes which derive synchronization directly from the data signal. However, these techniques have application in burst systems such as spacecraft command systems, and in systems where receiver simplicity is more important than power conservation. For systems in which the composite data-clock signal subsequently modulates an RF carrier, it is shown that the summed clock signal performs slightly better than the AM clock signal, and that for both signal types, the optimum allocation of power between data and clock is approximately 9:1.

Geist, J. M.↗

NASA NTRS1979

Synchronization of clocks by very-long-baseline interferometry

Two hydrogen-maser clocks, one at Haystack Observatory and one at the National Radio Astronomy Observatory, were synchronized by means of observations of several extragalactic radio sources on March 28, and again on September 23, 1977. Observations were made sequentially in eight 360-kHz bands distributed between about 8.4 and 8.5 GHz with spacings designed to enable the group-delay difference between the signals received at the two observatories from a given source to be estimated unambiguously, within an uncertainty of less than 1 ns set by receiver noise. The epoch and the rate differences between the observatories' clocks for each experiment were estimated by analysis of observations that spanned several hours. The application of corrections for the contributions to the delays of the antennas, feeds, receiver systems, and recorders yielded absolute determinations of the clock epoch differences. During each experiment, portable cesium clocks were flown from the U.S. Naval Observatory to the observatories and back. The traveling-clock data, analyzed in each case after the VLBI synchronization had been completed, confirmed the VLBI results to within 18 and 14 ns for the first and second experiments, respectively.

Clark, T. A.↗

NASA NTRS1982

Field operations with cesium clocks in HF navigation systems

Networks of HF phase comparison marine navigation stations employing cesium clocks are discussed. The largest permanent network is in the Gulf of Mexico where some fourteen base stations are continuously active and others are activated as needed. These HF phase comparison systems, which operate on a single transmission path, require a clock on the mobile unit as well. Inventory consists of upwards of 70 clocks from two different manufacturers. The maintenance of this network as an operating system requires a coordinated effort involving clock preparation, clock environment control, station performance monitoring and field service.

Christy, E. H.↗

NASA NTRS1985

Ensuring fault tolerance of phase-locked clocks

Processors within a real-time multiprocessor system must be synchronized with as little overhead as possible. Although synchronization can be achieved via both software (e.g., interactive convergence and interactive consistency algorithms) and hardware (e.g., multistage synchronizers and phase-locked clocks), phase-locked clocks are most attractive due to their small overheads. Despite the fact that synchronization of the multiprocessor system with phase-locked clocks is totally different in nature from the interactive consistency algorithm, it is presently proven that it must satisfy the same condition, N equal to or greater than 3m + 1, where N is the total number of clocks in the multiprocessor system and m is the maximum number of faults tolerable. Also presented are results showing how to design phase-locked clocks so as to be impervious up to a given arbitrary number of malicious failures.

Krishna, C. M.↗

NASA NTRS1987

CMOS Clock Synchronizer

Circuit synchronizes clock and gate signals within one-quarter of clock cycle. Clock synchronizer with one-quarter-cycle skew constructed from three flip-flops, three NAND gates, and inverter. In addition gate signal to which clock synchronized, circuit requires square-wave input at twice desired clock frequency.

Kepp, R. B.↗

NASA NTRS1987

Clock synchronization of a large multiprocessor system in the presence of malicious faults

An interconnection algorithm is presented for achieving clock synchronization in a multiprocessor system. The system is assumed to be maliciously faulty, i.e., some processors are out of synchronization and lie about their clock state to other intragroup or intergroup processors. A phase-locked clock network design is proposed which groups the clocks in the system into diverse clusters. The clusters are then treated as single clock units from the perspective of the network. The algorithm minimizes the number of interconnections while permitting synchronization of large multiprocessor systems controlling time-critical applications such as aircraft, nuclear reactors and industrial processes.

Shin, Kang G.↗

NASA NTRS1990

Real-time simulation clock

The invention is a clock for synchronizing operations within a high-speed, distributed data processing network. The clock is actually a distributed system comprising a central clock and multiple site clock interface units (SCIUs) which are connected by means of a fiber optic star network and which operate under control of separate clock software. The presently preferred embodiment is a part of the flight simulation system now in current use at the NASA Langley Research Center.

Bennington, Donald R.↗

NASA NTRS1992

Test of an orbiting hydrogen maser clock system using laser time transfer

We describe a joint Smithsonian Astrophysical Laboratory/National Aeronautics and Space Administration (SAO/NASA) program for flight testing a atomic hydrogen maser clock system designed for long-term operation in space. The clock system will be carried by a shuttle-launched EURECA spacecraft. Comparisons with earth clocks to measure the clock's long-term frequency stability (tau = 10(exp 4) seconds) will be made using laser time transfer from existing NASA laser tracking stations. We describe the design of the maser clock and its control systems, and the laser timing technique. We describe the precision of station time synchronization and the limitations in the comparison between the earth and space time scales owing to gravitational and relativistic effects. We will explore the implications of determining the spacecraft's location by an on-board Global Position System (GPS) receiver, and of using microwave techniques for time and frequency transfer.

Vessot, Robert F. C.↗

NASA NTRS1993

RighTime: A real time clock correcting program for MS-DOS-based computer systems

A computer program is described which effectively eliminates the misgivings of the DOS system clock in PC/AT-class computers. RighTime is a small, sophisticated memory-resident program that automatically corrects both the DOS system clock and the hardware 'CMOS' real time clock (RTC) in real time. RighTime learns what corrections are required without operator interaction beyond the occasional accurate time set. Both warm (power on) and cool (power off) errors are corrected, usually yielding better than one part per million accuracy in the typical desktop computer with no additional hardware, and RighTime increases the system clock resolution from approximately 0.0549 second to 0.01 second. Program tools are also available which allow visualization of RighTime's actions, verification of its performance, display of its history log, and which provide data for graphing of the system clock behavior. The program has found application in a wide variety of industries, including astronomy, satellite tracking, communications, broadcasting, transportation, public utilities, manufacturing, medicine, and the military.

Becker, G. Thomas↗

NASA NTRS1993

Estimating the instabilities of N clocks by means of comparison measurements

The estimation of individual instabilities of N clocks, compared by measuring the differences of their readings, is considered without assuming a priori any hypotheses on their uncorrelation. Instabilities of the N clocks are described by a complete (non-diagonal) N x N covariance matrix R. Only differences of clock readings are available in order to estimate R. Statistical processing of these data allows one to calculate the (N-1)x(N-l) covariance matrix S of the differences relative to the N-th(reference) clock. By analyzing the relationships tying R and S, several pieces of information can be inferred and, in particular, the conditions for the validity of the uncorrelation hypothesis are established. The estimation of R from S is not unique: in any case R must be positive definite. A theorem states that R is positive definite if and only if its determinant is positive. Nevertheless infinitely many acceptable choices of R still fulfill the condition of positive definiteness. This paper shows that, by increasing the number N of compared clocks, the amount of arbitrariness in estimating R is reduced. The analysis of some experimental data illustrates the capability of the method.

Premoli, Amedeo↗