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Cryo-EM structures of Clostridium perfringens enterotoxin bound to its human receptor, claudin-4
Not Available
Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution
Not provided.
Cryo-EM captures the coordination of asymmetric electron transfer through a di-copper site in DPOR
Enzymes that catalyze long-range electron transfer (ET) reactions often function as higher order complexes that possess two structurally symmetrical halves. The functional advantages for such an architecture remain a mystery. Using cryoelectron microscopy we capture snapshots of the nitrogenase-like dark-operative protochlorophyllide oxidoreductase (DPOR) during substrate binding and turnover. DPOR catalyzes reduction of the C17 = C18 double bond in protochlorophyllide during the dark chlorophyll biosynthetic pathway. DPOR is composed of electron donor (L-protein) and acceptor (NB-protein) component proteins that transiently form a complex in the presence of ATP to facilitate ET. NB-protein is an α 2 β 2 heterotetramer with two structurally identical halves. However, our structures reveal that NB-protein becomes functionally asymmetric upon substrate binding. Asymmetry results in allosteric inhibition of L-protein engagement and ET in one half. Residues that form a conduit for ET are aligned in one half while misaligned in the other. An ATP hydrolysis-coupled conformational switch is triggered once ET is accomplished in one half. These structural changes are then relayed to the other half through a di-nuclear copper center at the tetrameric interface of the NB-protein and leads to activation of ET and substrate reduction. These findings provide a mechanistic blueprint for regulation of long-range electron transfer reactions.
Cryo-EM reveals a composite flavobicluster electron bifurcation site in the Bfu family member NfnABC
Not provided.
Automated Cryo-TEM of Highly Beam-Sensitive Hard-Soft Ionomer Interfaces in Green Hydrogen Devices
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Research investigations of bulkhead cylindri- cal junctions exposed to combined load, cryo- genic temperature. part iv- theoretical analysis of the buckling problem
Compression buckling of shell of revolution under axial compression - shell stability
Pilot cryo tunnel: Attachments, seals, and insulation
Several different tests are described which simulated the actual configuration of a cryogenic wind tunnel operating at pressures up to 5 atmospheres (507 kPa) and temperatures from -320 F (78K) to 120 F (322K) in order to determine compatible bolting, adequate sealing, and effective insulating materials. The evaluation of flange attachments (continuous threaded studs) considered bolting based on compatible flanges, attachment materials, and prescribed bolt elongations. Various types of seals and seal configurations were studied to determine suitability and reusability under the imposed pressure and temperature loadings. The temperature profile was established for several materials used for structural supports.
Shock testing of a nitrogen tank (Cryo diffusion type 220 VLR) on the middle weight shock stand
Results of vertical and horizontal shock tests on type 200 VLR nitrogen tank are given.
The cryo-testing of infrared filters and beamsplitters for the cosmic background explorer's instruments
The cryooptical methods used to measure the spectral transmittances of filters and beamsplitters for the Cosmic Background Explorer's instruments are described. Measured results demonstrate the temperature sensitivity, or insensitivity, of various infrared filter designs within the wavelength range from 1 to 1000 microns.
A 2.3-GHz low-noise cryo-FET amplifier
A cryogenic cooled, low-noise Field Effect Transistor (FET) amplifier assembly for use at 2.2 to 2.3 GHz was developed for the DSN to meet the requirements of a Very Long Baseline Interferometry (VLBI) upgrade. An amplifier assembly was developed at JPL that uses a commercial closed-cycle helium refrigerator (CCR) to cool a FET amplifier to an operating temperature of 15 K. A cooled probe waveguide-to-coaxial transition similar to that used in the research and development Ultra-Low-Noise S-band Traveling Wave Maser (TWM) is used to minimize input line losses. Typical performance includes an input flange equivalent noise contribution of 14.5 K, a gain slope of less than 0.05 dB/MHz across a bandwidth of 2.2 to 2.3 GHz, an input VSWR of 1.5:1 at 2.25 GHz, and an insertion gain of 45 + or - 1 dB across the bandwidth of 2.2 to 2.3 GHz. Three 2.3 GHz FET/CCR assemblies were delivered to the DSN in the spring of 1987.
Advanced cryo propulsion systems
The following topics are presented in viewgraph form: (1) advanced space engine (ASE) chronology; (2) an ASE description; (3) a single expander; (4) a dual expander; (5) split expander; (6) launch vehicle start; (7) space start; (8) chemical transfer propulsion; and (9) an advanced expander test bed.
Cryo-mechanical tests of Ames 24E2 IR-black coating
In addition to the ambient condition tape test, five mechanical tests of the IR-black coating, Ames 24E2, were performed at either liquid helium or liquid nitrogen temperatures. Tensile strain in the coating at liquid nitrogen temperature was measured up to values of 4E-3, both before and after the coating was cycled down to liquid helium temperature. When applied to an aluminum substrate which was then bent in liquid nitrogen, the aluminum substrate always failed (permanently deformed) well before the coating failed. Sinusoidal accelerations up to 45 Gs in liquid nitrogen and 25 Gs in liquid helium did not crack or otherwise visibly damage the coating. Both sinusoidal and random acceleration at about 90 K of a representative baffle vane structure, at frequencies from 10 to 2000 Hz and up to 15 Gs, did not damage the coating, even at the intersection of a baffle with the telescope tube. Thus on a macroscopic level, cryogenic cooling and various levels of acceleration and strain did not affect this coating. However, on a microscopic scale, some loose particles were found associated with several tests. Outgassing data are also given.
Multi stage lunar lander storable versus cryo propulsion
Space transfer vehicle concepts and requirements are examined in a viewgraph format. Topics covered include the following: stage interfaces, element design, ascent and descent systems, and performance parameters.
Cryo-mechanical tests of Ames 24E2 IR-black coating
In addition to the ambient condition tape test, five mechanical tests of the IR-black coating, Ames 24E2, were performed at either liquid helium or liquid nitrogen teperatures. Tensile strain in the coating at liquid nitrogen temperature was measured up to values of 4E-3, both before and after the coating was cycled down to liquid helium temperature. When applied to an aluminum substrate which was then bent in liquid nitrogen, the aluminum substrate always failed (permanently deformed) well before the coating failed. Sinusoidal accelerations up to 45 Gs in liquid nitrogen and 25 Gs in liquid helium did not crack or otherwise visibly damage the coating. Both sinusoidal and random acceleration at about 90 K of a representative baffle vane structure, at frequencies from 10 to 2000 Hz and up to 15 Gs, did not damage the coating, even at the intersection of a baffle with the telescope tube. Thus on a macroscopic level, cryogenic cooling and various levels of acceleration and strain did not affect this coating. However, on a microscopic scale, some loose particles were found associated with several tests. Outgassing data are also given
Catalog of infrared and cryo-optical properties for selected materials
This report will describe the status of an effort to catalog the refractive index, absorption, reflectance and transmittance of selected IR transmitting materials over the wavelength range from 1 to 1000 microns, and at temperatures from 300 to about 2 K. Data are presented for LiF, CaF2, BaF2, KBr, and TlBr, as well as for KRS-5 (TlBr-I) and KRS-6 (TlBr-Cl). A cryooptical measurements facility at NASA-Goddard, which has been used to generate some of the cataloged data, is also described.
Cryo-Cooled Sapphire Oscillator for the Cassini Ka-Band Experiment
We present features for an ultra-stable sapphire cryogenic oscillator which has been designed to support the Cassini Ka-band Radio Science experiment. The design of this standard is new in several respects. It is cooled by a commercial cryocooler instead of liquid cryogens to increase operating time, and it uses a technology to adjust the temperature turn-over point to extend the upper operating temperature limit and to enable construction of multiple units with uniform operating characteristics. Objectives are 3 x 10(exp -15) stability for measuring times 1 second less than or equal to (tau) less than or equal to 100 seconds, phase noise of -85 dBc/Hz from offset frequencies of 1 Hz to 1000 Hz at 10 GHz carrier frequency, and a one year continuous operating period.