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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 109 records · Page 6

Crystal Structure of a CRISPR RNA-guided Surveillance Complex Bound to a ssDNA Target

In prokaryotes, RNA derived from type I and type III CRISPR loci direct large ribonucleoprotein complexes to destroy invading bacteriophage and plasmids. In Escherichia coli, this 405-kilodalton complex is called Cascade. We report the crystal structure of Cascade bound to a single-stranded DNA (ssDNA) target at a resolution of 3.03 angstroms. The structure reveals that the CRISPR RNA and target strands do not form a double helix but instead adopt an underwound ribbon-like structure. This noncanonical structure is facilitated by rotation of every sixth nucleotide out of the RNA-DNA hybrid and is stabilized by the highly interlocked organization of protein subunits. These studies provide insight into both the assembly and the activity of this complex and suggest a mechanism to enforce fidelity of target binding.

59 BASIC BIOLOGICAL SCIENCES↗

The MFiX Particle-in-Cell Method (MFiX-PIC) Theory Guide

MFiX (Multiphase Flow with Interphase eXchanges) is an open-source multiphase flow solver developed at the National Energy Technology Laboratory. Within the code, users have access to a single phase or interpenetrating continua-based multiphase two-fluid model (TFM), a discrete element model (DEM), and a particle-in-cell model (PIC). TFM, DEM, and PIC can all be used to create multiphase simulations that include hydrodynamics, chemical reactions, and heat transfer.

01 COAL, LIGNITE, AND PEAT↗

Multi-processing control system for the SEL 840MP (MPCS/1) users guide. Volume 1: Programming guide

A programmer's manual is presented for the SEL 840MP multiprocessing control system MPCS. The topics discussed include task control; defining displays; MPCS calls for task control functions; MPCS calls and system interfaces for display functions; MPCS calls for input/output functions and services, file management, and utility services; and MPCS assembly language call sequence summary.

Source record↗

DORMAN computer program (study 2.5). Volume 2: User's guide and programmer's guide

The DORMAN program was developed to create and modify a data bank containing data decks which serve as input to the DORCA Computer Program. Via a remote terminal a user can access the bank, extract any data deck, modify that deck, output the modified deck to be input to the DORCA program, and save the modified deck in the data bank. This computer program is an assist in the utilization of the DORCA program. The program is dimensionless and operates almost entirely in integer mode. The program was developed on the CDC 6400/7600 complex for implementation on a UNIVAC 1108 computer.

Wray, S. T., Jr.↗

Studies of aircraft differential maneuvering. Report 75-27: Calculating of differential-turning barrier surfaces. Report 75-26: A user's guide to the aircraft energy-turn and tandem-motion computer programs. Report 75-7: A user's guide to the aircraft energy-turn hodograph program

The numerical analysis of composite differential-turn trajectory pairs was studied for 'fast-evader' and 'neutral-evader' attitude dynamics idealization for attack aircraft. Transversality and generalized corner conditions are examined and the joining of trajectory segments discussed. A criterion is given for the screening of 'tandem-motion' trajectory segments. Main focus is upon the computation of barrier surfaces. Fortunately, from a computational viewpoint, the trajectory pairs defining these surfaces need not be calculated completely, the final subarc of multiple-subarc pairs not being required. Some calculations for pairs of example aircraft are presented. A computer program used to perform the calculations is included.

Kelley, H. J.↗

Geologic guide to the island of Hawaii: A field guide for comparative planetary geology

With geological data available for all inner planets except Venus, we are entering an era of true comparative planetary geology, when knowledge of the differences and similarities for classes of structures (e.g., shield volcanoes) will lead to a better understanding of general geological processes, regardless of planet. Thus, it is imperative that planetologists, particularly those involved in geological mapping and surface feature analysis for terrestrial planets, be familiar with volcanic terrain in terms of its origin, structure, and morphology. One means of gaining this experience is through field trips in volcanic terrains - hence, the Planetology Conference in Hawaii. In addition, discussions with volcanologists at the conference provide an important basis for establishing communications between the two fields that will facilitate comparative studies as more data become available.

Greeley, R.↗

Multisatellite attitude determination/optical aspect bias determination (MSAD/OABIAS) system description and operating guide. Volume 3: Operating guide

The Multisatellite Attitude Determination/Optical Aspect Bias Determination (MSAD/OABIAS) System, designed to determine spin axis orientation and biases in the alignment or performance of optical or infrared horizon sensors and Sun sensors used for spacecraft attitude determination, is described. MSAD/OABIAS uses any combination of eight observation models to process data from a single onboard horizon sensor and Sun sensor to determine simultaneously the two components of the attitude of the spacecraft, the initial phase of the Sun sensor, the spin rate, seven sensor biases, and the orbital in-track error associated with the spacecraft ephemeris information supplied to the system. In addition, the MSAD/OABIAS system provides a data simulator for system and performance testing, an independent deterministic attitude system for preprocessing and independent testing of biases determined, and a multipurpose data prediction and comparison system.

Joseph, M.↗

Exploring the Effects of Guide Vane on Dimpled Cooling Channel of Gas Turbine Blade

Abstract The purpose of the current study is to find out the heat transfer and pressure drop phenomena of the cooling channel with dimples and guide vane and compare the results with the no guide vane dimpled cooling channel. The first leg of the cooling channel is 490 mm, and the second leg is 460 mm. The two legs relate to the 180 deg turn region. The guide vane was inserted at the bend region of the dimpled cooling channel. The study was conducted with two different guide vanes geometry at two different orientations, i.e., U-guide vane with protrusion and depression orientation and curve-guide vane with protrusion and depression orientations both experimentally and numerically. The numerical study was performed with the large eddy simulation method. The result shows that for both stationary and rotational motion, the U-guide vane with depression experiences the highest thermal performance. The friction factor is comparatively higher for curve-guide vane with protrusion under stationary motion. However, under rotation, the curve-protrusion guide encounters the highest friction factor, which is higher compared to the no guide vane cooling channel.

Energy & Fuels↗