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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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94 records · Page 6

Effects of elevated atmospheric carbon dioxide concentrations on water and acid requirements of soybeans grown in a recirculating hydroponic system

Establishing mass budgets of various crop needs, i.e. water and nutrients, in different environments is essential for the Controlled Ecological Life Support System (CELSS). The effects of CO2 (500 and 1000 umol mol (exp -1)) on water and acid use (for pH control) by soybeans in a recirculating hydroponic system were examined. Plants of cvs. McCall and Pixie were grown for 90 days using the nutrient film technique (NFT) and a nitrate based nutrient solution. System acid use for both CO2 levels peaked near 4 weeks during a phase of rapid vegetative growth, but acid use decreased more rapidly under 500 compared to 1000 umol mol (exp GR) CO2. Total system water use by 500 and 1000 umol mol (exp -1) plants was similar, leaving off at 5 weeks and declining as plants senesced (ca. 9 weeks). However, single leaf transpiration rates were consistently lower at 1000 umol mol (exp -1). The data suggest that high CO2 concentrations increase system acid (and nutrient) use because of increased vegetative growth, which in turn negates the benefit of reduced water use (lower transpiration rates) per unit leaf area.

Mackowiak, C. L.↗

Elemental profiling and genomewide association studies reveal genomic variants modulating ionomic composition in Populus trichocarpa leaves

Samples were collected from a population of 1,089 black cottonwood genotypes (P. trichocarpa) assembled from native stands to encompass the central portion of the natural range of the species, stretching from 38.8° to 54.3° N Q13 latitude from California, USA, to British Columbia, Canada. Establishment of the common garden, growth conditions, and site maintenance have been described by Muchero et al (2015). In this study, leaf samples for ionomic profiling were collected from 4-year-old trees, during the growing season, in July 2012, from a field located in Clatskanie, Oregon, USA (46°6′11″N 123°12′13″W). The field site was located in a protected alluvial floodplain containing a uniform Wauna-Locoda silt loam soil area characterized by an acidic pH, in Columbia County, Oregon. A subset of 584 out of the 1,089 P. trichocarpa genotypes were represented in this sampling. These genotypes were randomly selected to represent the geographical distribution of the population. A single fully mature (LPI 7-9) leaf on the south side of the tree exposed to full sunlight conditions was removed from the tree within a 6-hour window centering on solar noon and immediately frozen under dry ice before processing. Leaf samples of 584 P. trichocarpa genotypes were finely ground to 40 mm particle size using a mortar and pestle, and ionomic composition was analyzed using ICP-MS. In total, 20 elements were profiled, including aluminum (Al27), arsenic (As75), boron (B11), cadmium (Cd111), calcium (Ca43), cobalt (Co), copper (Cu), iron (Fe57), magnesium (Mg25), manganese (Mn55), molybdenum (Mo), nickel (Ni60), phosphorus (P31), potassium (K39), rubidium (Rb85), selenium (Se82), sodium (Na23), strontium (Sr88), sulfur (S34), and zinc (Zn66), following a protocol established by Ziegler et al. (2013). For each sample, 75mg of powder was digested overnight in 2.5 mL HNO3 containing 20 parts per billion (ppb) indium as an internal standard, following the protocol described in Ziegler et al. (2013). Following a dilution, concentration of the 20 elements was measured using an Elan 6000 DRC-e mass spectrometer (Perkin-Elmer SCIEX) connected to a PFA microflow nebulizer (Elemental Scientific) and Apex HF desolvator (Elemental Scientific). One measurement per sample per genotype was done. For subsequent analyses, the quantifications were converted to total element concentration.

CBI ionomics, GWAS, plasma-mass spectrometry, neut↗

Lunar Plant Experiment: Testing Plant Germination in Lunar Gravity and Ionizing Radiation

We present an open design for a first plant growth module on the Moon (LPX). The primary science goal of lunar habitat is to investigate germination and initial plant growth when subject to the combined effects of lunar gravity and lunar surface radiation. The LPX module has been designed to be a flexible base unit that can be adapted to fly to the lunar surface on a variety of landers and rovers. LPX has size and shape of a 1 Unit CubeSat (10 centimeters on a side) and a total mass of 0.3 kilograms. The base will contain a seed module holding around 50 Arabidopsis seeds and provide approximately 0.5 liters of normal air at standard pressure. By the action of a small pump, water will be released after landing to initiate germination. Images will document the germination, initial growth, phototropism, and circumnutation of the small plants while sunlight remains available. A CO2 sensor tracks the release of CO2 during germination and the subsequent uptake of CO2 by photosynthesis. Simulations indicate that the CO2 peak level will be an increase by 2250 parts per million after 2 earth-days and then decline to about 1000 parts per million after 7 earth-days. The camera and CO2 sensor interfaces are USB. The water pump initiation requires a 5-volt signal. After initiation of germination by the addition of water, the habitat must be maintained above 22 degrees Centigrade to allow for plant growth and below 27 degrees Centigrade to prevent damage to the plants. This relatively tight temperature tolerance will require thermal control systems using reflecting surfaces and insulation that must be specifically designed for each lander or rover. The plant growth unit will function to tilt angles of 20 degrees along any axis. Total image data volume required to gauge the plant growth rate and leaf area is 1 megabyte (minimum data required) to 40 megabytes (preferred data). The lid of the habitat will allow adequate sunlight for plant growth. To provide for optimum growth, light levels in the plant growth stage need to be between 75 and 150 micromoles per meter squared per second interval.

first plant growth↗

Litter Production of Oak-Hickory Forest at Missouri Ozark (MOFLUX) Site: 2003-2022

This dataset contains measurements of litter production at the second-growth upland oak-hickory forest at the Missouri Ozark AmeriFlux (MOFLUX) site. The MOFLUX site is located in the University of Missouri Baskett Forest situated in the Ozark Border Region of central Missouri, USA, and is part of the AmeriFlux network (site ID: US-MOz). During 2003, 24 circular vegetation plots (each 0.08 hectares) were established in the MOFLUX forest. The plots were situated 50 meters apart on 5 linear transects radiating out from the flux tower base in southeast, south, southwest, west, and northwest directions—there were 5 plots per transect except for the northwest one, which had only 4 due to the presence of a small pond at the terminus (Fig. 1). Litter measurements were initiated in October 2003 and continue to the present. Data through 2022 are reported in this dataset. On each plot, two subplots were established for the collection of litter. Subplots were setup using a stratified random design to ensure the collectors were not immediately adjacent to one another. Litter collectors were usually emptied at monthly intervals, or less during the autumn leaf drop. Samples were dried to constant mass and then sorted before determining the dry mass of leaf, vegetative and woody litter. These litter data have been valuable in modeling efforts to examine the impacts of intensifying moisture extremes (i.e., moisture excess and deficit conditions) on soil carbon cycling. More recent data will be added periodically with no changes to previously released data or the format and structure of the data file. This dataset contains one data file in comma separate (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

54 ENVIRONMENTAL SCIENCES↗