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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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103 records · Page 6

Mesocarnivore Surveys on Lawrence Livermore National Laboratory Site 300 Alameda and San Joaquin Counties, California

Lawrence Livermore National Laboratory (LLNL), operated under cooperative agreement between the University of California and the U. S. Department of Energy, administers and operates an approximately 11 mi2 (2$ km2) test site in the remote hills at the northern end of the South Coast Ranges of Central California. Known as Site 300, this expanse of rolling hills and canyons supports a diverse array of grassland communities typical of lowland central California. The facility serves a variety of functions related to testing non-nuclear explosives, lasers, and weapons subsystems. The primary purpose of this project was to determine the presence of any mesocamivores on Site 300 that use the property for foraging, denning, and other related activities. The surveys occurred from mid-September to mid-October, 2002.

54 ENVIRONMENTAL SCIENCES↗

Los Alamos National Laboratory Floodplain Assessment for Fire Risk Mitigations at the Lower Slobbovia Testing Site in (TA-36)

The National Nuclear Security Administration (NNSA), a semi-autonomous agency within the U.S. Department of Energy (DOE), is proposing to take fire pre-suppression actions at Los Alamos National Laboratory (LANL) Technical Area (TA) 36. Constructing a fire break and a fuel break surrounding the Lower Slobbovia explosives testing site in addition to the current vegetation mowing practices would establish a safety perimeter to help prevent fires from spreading away from the firing point. NNSA has prepared this floodplain assessment in accordance with 10 Code of Federal Regulations (CFR) Part 1022 Compliance with Floodplain and Wetland Environmental Review Requirements (10 CFR 1022), which was promulgated to implement DOE requirements under Executive Order 11988 Floodplain Management (EO 1977). A floodplain is defined in 10 CFR 1022 as “the lowlands adjoining inland and coastal waters and relatively flat areas and flood prone areas of offshore islands,” and a base floodplain as “the 100-year floodplain, that is, a floodplain with a 1.0 percent chance of flooding in any given year (CFR 2003).” This floodplain assessment evaluates potential impacts to floodplain values and functions from implementation of the proposed action, identifies alternatives to the Proposed Action, and allows for meaningful public comment.

54 ENVIRONMENTAL SCIENCES↗

Los Alamos National Laboratory Wetland Assessment for the Replacement Water Lines from Technical Area 48 to Technical Area 55 Project

The National Nuclear Security Administration (NNSA), a semi-autonomous agency within the U.S. Department of Energy (DOE), is proposing to take action at Los Alamos National Laboratory (LANL) near a wetland at Technical Area (TA) 48 and under a wetland at TA-55. The project proposes to install two 12-inch water lines along an alignment from TA-48 to Pecos Road on the east side of TA-55 (Figure 1). The proposed water lines will replace aging cast iron pipes that provide water for fire suppression to buildings within TA-55, and potable water to other areas in the Pajarito Corridor. NNSA has prepared this wetland assessment in accordance with 10 Code of Federal Regulations (CFR) Part 1022 Compliance with Wetland and Wetland Environmental Review Requirements (10 CFR Part 1022) (CFR 2003) which was promulgated to implement DOE requirements under Executive Order 11988 Wetland Management (EO 1977). A wetland is defined in 10 CFR 1022 as “the lowlands adjoining inland and coastal waters and relatively flat areas and flood prone areas of offshore islands,” and a base wetland as “the 100-year wetland, that is, a wetland with a 1.0 percent chance of flooding in any given year (CFR 2003).” This wetland assessment evaluates potential impacts to wetland values and functions from implementation of the proposed action, identifies alternatives to the Proposed Action, and allows for meaningful public comment.

54 ENVIRONMENTAL SCIENCES↗

Genetics and Genomics of Pathogen Resistance in Switchgrass (Final Report)

This project was funded by DOE under Grant no. DE-SC0016108. Originally approved for the 2016-2019 period, two no-cost extensions were solicited and approved, which prolonged the lifespan through July 2021. This final report informs on the results obtained so far from the research implemented. The research hinged on integrating genomics (genomic selection, RNAseq, virus-plant interactions) with classical genetics (conventional breeding) to incorporate durable resistance to fungal (rust) and viral (mosaic) diseases in switchgrass (Panicum virgatum) populations being bred for bioenergy. Higher biomass yield, higher quality (low lignin content), and durable disease resistance are key features to make lignocellulosic switchgrass feedstocks economically competitive and sustainable. Genomic selection is being applied on three generations of a switchgrass population derived from crossing two ecotypes (Kanlow as lowland female and Summer as upland male) with differential performance in terms of biomass yield and quality, disease resistance, and winter survivability. Target populations were screened for rust and mosaic in field and/or lab and phenotyped for biomass yield and quality traits. Genetic analyses were applied across generations to capture the joint inheritance of the targeted traits and predict breeding values for parents and progeny with greater accuracy. Parental and a panel of different switchgrass populations were genotyped with the DArTseq technology to develop SNP (0, 1, 2) and in-silico (presence/absence) DArT markers. Rust inoculations techniques were developed and applied successfully on switchgrass. The original populations (Kanlow and Summer) were sequenced with RNAseq to capture the gene expression profiles across sequential time-points and appraise the basis of greater resistance in the Kanlow vs the Summer ecotype. Constructs of PMV and sPMV mosaic virus were assembled and tested first on proso millet to find the best protocol to use later on switchgrass. Results from the preliminary analyses indicate that 1) ample additive genetic variation is available for selection and improving this inter-ecotypic population for yield, quality, and disease traits, 2) significant gains are to be expected with the genetic correlations being favorable between yield and lignin content and between yield and disease ratings, 3) substantial differences exist in the genetic regions controlling rust resistance in the two ecotypes, 4) co-infection with PMV isolates from Nebraska and its satellite from Kansas elicit severe mosaic symptoms, and 5) two different genetic systems are responsible for imparting resistance to rust and virus in switchgrass.

59 BASIC BIOLOGICAL SCIENCES↗

How does Mercury Methylation Respond to Intensive Forest Management and the Creation of Anoxia in Floodplain Soils

This research endeavor, entitled “How does Mercury Methylation Respond to Intensive Forest Management and the Creation of Anoxia in Floodplain Soils?” represents a collaborative effort undertaken by the University of North Carolina at Greensboro, led by Principal Investigator Dr. James S. Coleman, the United States Forest Service Santee Experimental Forest (SEF) Station Director Dr. Carl C. Trettin as Co-Principal Investigator, and The Chinese University of Hong Kong Dr. Martin Tsz-Ki Tsui and Dr. Alex T. Chow serving as Co-Principal Investigators. The overarching objective of this proposed project was to capture and elucidate the immediate and short-term effects of forest disturbances, namely clear-cutting and thinning, on the biogeochemical cycling of mercury (Hg) within the SEF in Huger, South Carolina. To accomplish this objective, a two-year exploratory study was proposed to conduct soil sampling and measurements of Hg and toxic methylmercury (MeHg), and soil physiochemical data within the experimental watershed (WS77) at SEF, following the clear-cutting process initiated for the preparation of the native longleaf pine restoration project by the USFS. Based on our mercury and hydrological data assessment, we concluded that the implementation of forest management practices, particularly mechanical thinning and clear-cutting, appears to enhance the potential for Hg methylation. Furthermore, these forest management practices may disrupt and alter the storage, mobility, and transformations of atmospherically deposited Hg in these lowland forests. We advocate for further research to comprehensively evaluate the impact of forest management on Hg cycling within forest ecosystems.

54 ENVIRONMENTAL SCIENCES↗

Los Alamos National Laboratory Floodplain Assessment for the Technical Area 8 to Technical Area 22 Natural Gas Line Replacement Project

The National Nuclear Security Administration (NNSA), a semi-autonomous agency within the U.S. Department of Energy (DOE), is proposing new construction that crosses through upper Pajarito Canyon and upper Starmer Canyon from Technical Area (TA) 8 to TA-22 at Los Alamos National Laboratory (LANL). The project proposes to install a new 12 inch natural gas line to replace the existing 70+ year old natural gas line. Replacement will prevent loss of service to several LANL TAs and Bandelier National Monument. The project activities within the 100- year floodplains include 1) new natural gas line installation to replace and existing aging gas line, 2) relocating a section of fence and 3) three construction equipment and materials storage areas. NNSA has prepared this floodplain assessment in accordance with 10 Code of Federal Regulations (CFR) Part 1022 Compliance with Floodplain and Wetland Environmental Review Requirements (10 CFR Part 1022) (CFR 2003) which was promulgated to implement DOE requirements under Executive Order 11988 Floodplain Management (EO 1977). A floodplain is defined in 10 CFR 1022 as “the lowlands adjoining inland and coastal waters and relatively flat areas and flood prone areas of offshore islands,” and a base floodplain as “the 100-year floodplain, that is, a floodplain with a 1.0 percent chance of flooding in any given year (CFR 2003).” This floodplain assessment evaluates potential impacts to floodplain values and functions from implementation of the proposed action, identifies alternatives to the Proposed Action, and allows for meaningful public comment.

54 ENVIRONMENTAL SCIENCES↗

Heteronuclear single quantum coherence (HSQC) NMR spectra of lignin isolated from switchgrass residues after fermentation with milling

Here we present a curated dataset of two-dimensional heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) spectra of lignin isolated from a herbaceous energy crop (Panicum virgatum L.). The lowland variant “Timber” switchgrass from Ernst seeds was used. The switchgrass was knife milled and passed through a 2 mm sieve prior to consolidated bioprocessing (CBP) process. Switchgrass was suspended in Milli-Q water and autoclaved for 90 min on liquid cycles. The residues after autoclaving were then subjected to CBP using coculture of Clostridium thermocellum (C. thermocellum DSM 1313 (LL1004)) and Thermoanaerobacterium thermosaccarolyticum ( T. thermosaccharolyticum HG-8 ATCC 31960 (LL1244)). Once-fermented and twice-fermented (FF) switchgrass were subjected to ball and disc milling in bioreactors at 55 °C and 60, 48 grams/L solids loadings for primary and secondary fermentation respectively. When fermentations were completed, the residual solids were rinsed with milli-Q water. Lignin was isolated from the pretreated residues after ball-milling in a porcelain jar with ceramic balls via Retsch PM 200 at 600 rpm for 2 h followed by enzymatic hydrolysis in acetate buffer (pH 4.8, 50 °C) for 48 h. The dry lignin samples were dissolved in deuterated dimethyl sulfoxide (d6) and characterized using 13C–1H HSQC in a Bruker Avance III HD 500-MHz NMR spectrometer. A standard Bruker pulse sequence (hsqcetgpsisp.2) was used on a Prodigy platform cryoprobe. The spectra were acquired with the following acquisition conditions: 230 ppm spectral width in F1 (13C) dimension with 256 data points and 12 ppm spectral width in F2 (1H) dimension with 2048 data points, a 90° pulse, with a C–H coupling constant of 145 Hz, a 1.0 s pulse delay, and 64 scans. Spectra were processed using the Bruker TopSpin 3.6 software.

Lignin, HSQC, Switchgrass, CBP , Ball mill, Disc m↗

Trade-Offs in Phosphorus Acquisition Strategies of Five Common Tree Species in a Tropical Forest of Puerto Rico

Tree species that are successful in tropical lowlands have different acquisition strategies to overcome soil phosphorus (P) limitations. Some of these strategies belowground include adjustments in fine-root traits, such as morphology, architecture, association with arbuscular mycorrhizal fungi, and phosphatase activity. Trade-offs among P-acquisition strategies are expected because of their respective carbon cost. However, empirical evidence remains scarce which hinders our understanding of soil P-acquisition processes in tropical forests. Here, we measured seven fine-root functional traits related to P acquisition of five common tree species in three sites of the Luquillo Experimental Forest in Puerto Rico. We then described species-specific P-acquisition strategies and explored the changes in fine-root trait expression from 6 months before to 6 months after two consecutive hurricanes, Irma and María, passed over the island. We found that variations in root trait expression were driven mainly by the large interspecific differences across the three selected sites. In addition, we revealed a trade-off between highly colonized fine roots with high phosphatase activity and fine roots that have a high degree of branching. Furthermore, the former strategy was adopted by pioneer species ( Spathodea campanulata and Cecropia schreberiana ), whereas the latter was adopted by non-pioneer species (mostly Dacryodes excelsa and Prestoea montana ). Additionally, we found that root trait expression did not change comparing 6 months before and after the hurricanes, with the exception of root phosphatase activity. Altogether, our results suggest a combination of structural and physiological root traits for soil P acquisition in P-poor tropical soils by common tropical tree species, and show stability on most of the root trait expression after hurricane disturbances.

59 BASIC BIOLOGICAL SCIENCES↗

Root system architecture and environmental flux analysis in mature crops using 3D root mesocosms

Current methods of root sampling typically only obtain small or incomplete sections of root systems and do not capture their true complexity. To facilitate the visualization and analysis of full-sized plant root systems in 3-dimensions, we developed customized mesocosm growth containers. While highly scalable, the design presented here uses an internal volume of 45 ft 3 (1.27 m 3 ), suitable for large crop and bioenergy grass root systems to grow largely unconstrained. Furthermore, they allow for the excavation and preservation of 3-dimensional root system architecture (RSA), and facilitate the collection of time-resolved subterranean environmental data. Sensor arrays monitoring matric potential, temperature and CO 2 levels are buried in a grid formation at various depths to assess environmental fluxes at regular intervals. Methods of 3D data visualization of fluxes were developed to allow for comparison with root system architectural traits. Following harvest, the recovered root system can be digitally reconstructed in 3D through photogrammetry, which is an inexpensive method requiring only an appropriate studio space and a digital camera. We developed a pipeline to extract features from the 3D point clouds, or from derived skeletons that include point cloud voxel number as a proxy for biomass, total root system length, volume, depth, convex hull volume and solidity as a function of depth. Ground-truthing these features with biomass measurements from manually dissected root systems showed a high correlation. We evaluated switchgrass, maize, and sorghum root systems to highlight the capability for species wide comparisons. We focused on two switchgrass ecotypes, upland (VS16) and lowland (WBC3), in identical environments to demonstrate widely different root system architectures that may be indicative of core differences in their rhizoeconomic foraging strategies. Finally, we imposed a strong physiological water stress and manipulated the growth medium to demonstrate whole root system plasticity in response to environmental stimuli. Hence, these new “3D Root Mesocosms” and accompanying computational analysis provides a new paradigm for study of mature crop systems and the environmental fluxes that shape them.

59 BASIC BIOLOGICAL SCIENCES↗

Interplay of Topography, Fire History, and Climate on Interior Alaska Boreal Forest Vegetation Dynamics in the 21st Century: A Landsat Time-Series Analysis

This study investigates vegetation dynamics in boreal forests of Interior Alaska, focusing on topography, fire history, and climate influences. The study area includes Bonanza Creek Experimental Forest (BCEF) and surrounding region, categorized by topography (upland, floodplain, lowland) and fire history. Using Mann–Kendall trend and Theil–Sen slope analyses on Landsat-derived spectral metrics: Normalized Difference Vegetation Index (NDVI) and Normalized Burn Ratio (NBR), we observed a shift from browning to greening trends, particularly in historically burned areas. The photosynthetic activity in burned upland converged with unburned areas ~30 years post-fire, coincident with a shift towards deciduous dominance during post-fire succession. Normalized Difference Moisture Index (NDMI) trends revealed a significant increase in vegetation moisture content across all topographies. We introduce Effective Seasonal Precipitation Index (ESPI), which combines prior-year annual precipitation with current-year spring snow depth. Its positive correlation with NDMI highlights its potential for monitoring vegetation moisture dynamics at the landscape scale. Furthermore, by correlating dendrochronology-based climate indices, we found strong correlation between NDMI and normalized Supplemental Precipitation Index (nSPI), across topographies. Overall, this research provides critical insights into how climate and fire influence interior boreal vegetation, highlighting the effects of increased precipitation, and topography on shaping differential vegetation responses across the landscape.

Google Earth Engine↗

Development of inter-grid-cell lateral unsaturated and saturated flow model in the E3SM Land Model (v2.0)

Abstract. The lateral transport of water in the subsurface is important in modulating terrestrial water energy distribution. Although a few land surface models have recently included lateral saturated flow within and across grid cells, it is not a default configuration in the Climate Model Intercomparison Project version 6 experiments. In this work, we developed the lateral subsurface flow model within both unsaturated and saturated zones in the Energy Exascale Earth System Model (E3SM) Land Model version 2 (ELMv2.0). The new model, called ELMlat, was benchmarked against PFLOTRAN, a 3D subsurface flow and transport model, for three idealized hillslopes that included a convergent hillslope, divergent hillslope, and tilted V-shaped hillslope with variably saturated initial conditions. ELMlat showed comparable performance against PFLOTRAN in terms of capturing the dynamics of soil moisture and groundwater table for the three benchmark hillslope problems. Specifically, the mean absolute errors (MAEs) of the soil moisture in the top 10 layers between ELMlat and PFLOTRAN were within 1 %±3 %, and the MAEs of water table depth were within ±0.2 m. Next, ELMlat was applied to the Little Washita experimental watershed to assess its prediction of groundwater table, soil moisture, and soil temperature. The spatial pattern of simulated groundwater table depth agreed well with the global groundwater table benchmark dataset generated from a global model calibrated with long-term observations. The effects of lateral groundwater flow on the energy flux partitioning were more prominent in lowland areas with shallower groundwater tables, where the difference in simulated annual surface soil temperature could reach 0.3–0.4 ∘C between ELMv2.0 and ELMlat. Incorporating lateral subsurface flow in ELM improves the representation of the subsurface hydrology, which will provide a good basis for future large-scale applications.

58 GEOSCIENCES↗

A new large-scale suspended sediment model and its application over the United States

Abstract. Suspended sediment plays a vital role in the regional and global cycling of carbon and nutrients by carrying carbon and nutrients from headwaters into lowland rivers and the oceans. Sediment transport through river systems is often fundamentally modified by human activities such as reservoir management. However, a physically based representation of sediment transport is still missing in most existing Earth system models (ESMs), which are essential tools for modeling and predicting Earth system changes. Here, we introduce a multi-process river sediment module for ESMs, which includes (1) hillslope soil erosion and sediment discharge into streams, (2) sediment transport processes through river networks, (3) reservoir operation based on the inflows from upstream areas and water demand from downstream areas, and (4) sediment trapping by reservoirs. All model parameters are estimated a priori without calibration. We apply this new sediment modeling framework to the contiguous United States and validate it against historical observations of monthly streamflow and sediment discharges at 35 river gauges. The model reasonably well captures the long-term balance and seasonal variations of suspended sediment in large river systems. Furthermore, our model results show that suspended sediment discharge in managed rivers is affected more by reservoirs' direct trapping of sediment particles than by their flow regulation. This new sediment module enables future modeling of the transportation and transformation of carbon and nutrients carried by the fine sediment along the river–ocean continuum to close the global carbon and nutrient cycles.

54 ENVIRONMENTAL SCIENCES↗

Soil CO2 and CH4 Chamber Fluxes in Tussock Tundra, Council Road Mile Marker 71, Seward Peninsula, Alaska, 2016-2019

In August-September 2016, June-August 2017, June-August 2018, and June 2019, co-located measurements were made of surface CH4 and CO2 flux, soil temperature, moisture, and thaw depth. Measurements were made at 35 chamber locations at the Council Mile Marker 71 Site (CN_MM71) on the Seward Peninsula, Alaska. Chamber locations include upland moist acidic tussock tundra, thermo-erosional slopes, and periodically inundated lowland water channels. The dataset includes three *.csv data files with measurements using transparent and opaque chambers (CO2 and CH4 fluxes in light and dark) plus one *.pdf user guide.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗