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At least 109 records · Page 6

Supervised extraction of near-complete genomes from metagenomic samples: A new service in PATRIC

Large amounts of metagenomically-derived data are submitted to PATRIC for analysis. In the future, we expect even more jobs submitted to PATRIC will use metagenomic data. One in-demand use case is the extraction of near-complete draft genomes from assembled contigs of metagenomic origin. The PATRIC metagenome binning service utilizes the PATRIC database to furnish a large, diverse set of reference genomes. We provide a new service for supervised extraction and annotation of high-quality, near-complete genomes from metagenomically-derived contigs. Reference genomes are assigned to putative draft genome bins based on the presence of single-copy universal marker roles in the sample, and contigs are sorted into these bins by their similarity to reference genomes in PATRIC. Each set of binned contigs represents a draft genome that will be annotated by RASTtk in PATRIC. A structured-language binning report is provided containing quality measurements and taxonomic information about the contig bins. The PATRIC metagenome binning service emphasizes extraction of high-quality genomes for downstream analysis using other PATRIC tools and services. Due to its supervised nature, the binning service is not appropriate for mining novel or extremely low-coverage genomes from metagenomic samples.

59 BASIC BIOLOGICAL SCIENCES↗

Satellite Radar Altimetry for Monitoring Small Rivers and Lakes in Indonesia

Remote sensing and satellite geodetic observations are capable of hydrologic monitoring of freshwater resources. Although satellite radar altimetry has been used in monitoring water level or discharge, its use is often limited to monitoring large rivers ( >1 km) with longer interval periods (>1 week) because of its low temporal and spatial resolutions (i.e., satellite revisit period). Several studies have reported successful retrieval of water levels for small rivers as narrow as 40m. However, processing current satellite altimetry signals for such small water bodies to retrieve water levels accurately remains challenging. Physically, the radar signal returned by water bodies smaller than the satellite footprint is most likely contaminated by non-water surfaces, which may degrade the measurement quality. In order to address this scientific challenge, we carefully selected the waveform shapes corresponding to the range measurement resulting from standard retrackers for the European Space Agency's (ESA's) Envisat (Environmental Satellite) radar altimetry. We applied this approach to small (40-200m in width) and medium-sized (200-800m in width) rivers and small lakes (extent<1000 sq km) in the humid tropics of Southeast Asia, specifically in Indonesia. This is the first study that explored the ability of satellite altimetry to monitor small water bodies in Indonesia.

Satellite↗

Toxicological Assessment of the International Space Station Atmosphere

Space-faring crews must have safe breathing air throughout their missions to ensure adequate performance and good health. Toxicological assessment of air quality depends on the standards that define acceptable air quality, measurements of pollutant levels during the flight, and reports from the crew on their in-flight perceptions of air quality. Air samples from ISS flight 2A showed that contaminants in the Zarya module were at higher concentrations than the Unity module. At the crew's first entry, the amount of non-methane volatile organic compounds (NMVOCs) in Zarya was 23 Mg/cubic meter, whereas in the amount of NMVOCs in Unity was 5.3 mg/cubic meter. Approximately 26 hours later at egress from the modules, the NMVOCs were comparable indicating good mixing of the atmospheres. The 2A crew reported no adverse health effects related to air pollution during their flight. Ingress air samples from 2A.1, which was flown more than 5 months after 2A, again showed that the Zarya had accumulated more unscrubbed pollutants than Unity. The NMVOCs in Unity were 3.5 mg/cubic meter, whereas the were 20 mg/cubic meter in Zarya. After almost 80 hours of ISS operations, the NMVOCs were 7.5 and 12 mg/cubic meter in Unity and Zarya, respectively. This suggests that the atmospheres in the modules were not mixing very well. The 2A.1 crew felt that the air quality in Zarya deteriorated when they were working in a group at close quarters, when the panels had been removed, and after they had worked in an area for some time. The weight of evidence suggests that human metabolic products (carbon dioxide, water vapor, heat) were not being effectively removed from the crew's work area, and these caused their symptoms. Additional local measurements of pollutants are planned for the 2A.2 mission to the ISS.

James, John T.↗

Evaluation of flow quality in two large NASA wind tunnels at transonic speeds

Wind tunnel testing of low drag airfoils and basic transition studies at transonic speeds are designed to provide high quality aerodynamic data at high Reynolds numbers. This requires that the flow quality in facilities used for such research be excellent. To obtain a better understanding of the characteristics of facility disturbances and identification of their sources for possible facility modification, detailed flow quality measurements were made in two prospective NASA wind tunnels. Experimental results are presented of an extensive and systematic flow quality study of the settling chamber, test section, and diffuser in the Langley 8 foot transonic pressure tunnel and the Ames 12 foot pressure wind tunnel. Results indicate that the free stream velocity and pressure fluctuation levels in both facilities are low at subsonic speeds and are so high as to make it difficult to conduct meaningful boundary layer control and transition studies at transonic speeds.

Harvey, W. D.↗

The Spacecraft Mass Balance as a Diagnostic Tool for Cabin Air Quality

The spacecraft-level mass balance is a powerful tool for diagnosing and decoupling factors influencing cabin air quality, especially with respect to trace contaminant propagation. Successful implementation of this approach relies upon accurate and temporally relevant air quality measurements, which are often challenging to attain for emerging trace contaminants. In addition to the proper interpretation of air quality data trends, a thorough understanding of subsystem-level mass transfer is required to characterize process performance. For many Environmental Control and Life Support System processes, subsystem mass transfer may be predicted based on physicochemical properties and classic unit operation design approaches. Gaps in understanding of process performance should be supplemented with thorough ground testing. A high confidence in the fidelity of one of these two aspects, air quality data or process performance, may help offset uncertainties in the other. At minimum, thresholding assumptions and numerical methods can be imposed to constrain unknown parameters within the physical envelope of the situation at hand. Finally, the complexity of cabin-wide integrated mass balances may be reduced by implementing a phenomenological approach towards subsystem discretization.

Cabin Air-quality↗

Toxicological Assessment of the International Space Station Atmosphere with Emphasis on Metox Canister Regeneration

Space-faring crews must have safe breathing air throughout their missions to ensure adequate performance and good health. Toxicological assessment of air quality depends on the standards that define acceptable air quality, measurements of pollutant levels during the flight, and reports from the crew on their in-flight perceptions of air quality. Air samples returned from ISS on flights 8A, UF2, 9A, and 11A were analyzed for trace pollutants. On average, the air during this period of operations was safe for human respiration. However, about 3 hours into the regeneration of 2 Metox canisters in the U.S. airlock on 20 February 2002 the crew reported an intolerable odor that caused them to stop the regeneration, take refuge in the Russian segment, and scrub air in the U.S. segment for 30 hours. Analytical data from grab samples taken during the incident showed that the pollutants released were characteristic of nominal air pollutants, but were present in much higher concentrations. The odors reported by the crew were due to relatively high concentrations of n-butanol, and possibly other pollutants in the mixture. Later data taken during regeneration of Metox canisters that had not been subject to long-term flows showed minimal effects on air quality. Long-term trending data suggest that a disruption in atmospheric mixing between the Service Module and the U.S. Laboratory has occurred and that formaldehyde concentrations are gradually increasing in the U.S. Laboratory. Trending data also show that the releases of octafluoropropane (OFP) have subsided.

James, John T.↗

Qualification and Re-Qualification of NDA Instruments

Non-Destructive Assay (NDA) instruments are a large component of nuclear material (NM) measurement at LANL. Nuclear Material Control and Accountability Group (SAFE-NMCA) personnel in the Safeguards Division are responsible for oversight of the instruments used for material accountability measurements. It is the responsibility of SAFE-NMCA to ensure that all instruments are operating as expected and can produce quality measurements to provide assurance that requirements of the DOE approved NMCA Plan are being met.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Hemispheric Airborne Measurements of Air Quality (HAMAQ)

Under NASA’s Earth Venture Suborbital program, Hemispheric Airborne Measurements of Air Quality (HAMAQ) will conduct a series of campaigns in 2028 under the Tropospheric Emissions: Monitoring of Pollution (TEMPO) geostationary satellite instrument. HAMAQ plans include two deployments, including the Mexico City megalopolis and another North American site yet to be selected. The effort will include two aircraft, NASA’s B777 for in situ sampling and G-III for remote sensing. These aircraft will be used to complete a system of integrated observations, combining satellite observations, ground-based monitoring and research observations with air quality modeling. HAMAQ field intensives will serve multiple objectives to include: improving the use of satellite observations in concert with traditional ground monitoring to inform air quality; assessing emissions to better understand their timing and source apportionment; advancing the development of satellite proxies for air quality; and assessing the factors controlling local air quality in each location sampled. Given the long lead time for this campaign, this poster welcomes discussion from the community on strategies and candidate sites for the second deployment. Given the broad applicability of the HAMAQ science objectives and observing strategy, possible partnerships to extend the pursuit of the larger vision of HAMAQ to also sample in Asia and Europe are of interest.

James H Crawford↗

Mitigating coherent loss in superconducting circuits using molecular self-assembled monolayers

In planar superconducting circuits, decoherence due to materials imperfections, especially two-level-system (TLS) defects at different interfaces, is a primary hurdle for advancing quantum computing and sensing applications. Traditional methods for mitigating TLS loss, such as etching oxide layers at metal and substrate interfaces, have proven to be inadequate due to the persistent challenge of oxide regrowth. In this work, we introduce a novel approach that employs molecular self-assembled monolayers (SAMs) to chemically bind at different interfaces of superconducting circuits. This technique is specifically tested here on coplanar waveguide (CPW) resonators, in which this method not only impedes oxide regrowth after surface etching but can also tailors the dielectric properties at different resonators interfaces. The deployment of SAMs results in a consistent improvement in the measured quality factors across multiple resonators, surpassing those with only oxide-etched resonators. The efficiency of our approach is supported by microwave measurements of multiple devices conducted at millikelvin temperatures and correlated with detailed X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) characterizations of SAM-passivated resonators. The compatibility of SAMs materials with the established fabrication techniques offers a promising route to improve the performance of superconducting quantum devices.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

High Temperature Material Properties Measurement Capabilities of the MSFC Electrostatic Levitation (ESL) Laboratory

The NASA Marshall Space Flight Center (MSFC) electrostatic levitation (ESL) laboratory has a long history of providing materials research and thermophysical property data. The lab can measure thermophysical properties, such as density, surface tension, and viscosity of liquid materials, including elements, alloys, glasses, ceramics, and oxides. For improved measurement quality, the ESL lab also has an oxygen control system, which allows the oxygen partial pressure within the vacuum chamber to be measured and controlled, at elevated temperatures, over a wide range of partial pressures. The surface tension of metals is affected by even a small amount of adsorption of oxygen, and the presence of oxygen has been hypothesized as a likely cause for the large scatter seen in published surface tension data. This presentation will cover the MSFC ESL lab, its high temperature material properties measurement capabilities, and some information about measurements done on alloys relevant to additive manufacturing.

Michael SanSoucie↗

Comparison between Hayabusa 2 Spectral Measurements and Simulations

Recent Hayabusa 2 spectral measurements are compared with state-of-the-art shock-layer radiation simulations resulting from the LAURA/HARA code suite. These simulations include coupled ablation, which accounts for the injection of ablation products into the flowfield, and coupled radiation, which accounts for radiative energy loss in the flowfield. To enable the cou- pled ablation simulations, a best-estimate model is developed for Hayabusa’s carbon-phenolic ablator based on available published information. The comparison between the simulations and measurements focuses on three atomic nitrogen lines, two atomic oxygen lines, one atomic hydrogen line, and the CN Violet band system. Parametric uncertainties are evaluated for these features using a recently developed capability in LAURA/HARA. These uncertainties capture the impact of flowfield and radiation modeling uncertainties on the observed radiation simulations, and include the impact of coupled radiation and ablation product emission. The resulting parametric uncertainties range from 50 to over 100% for the atomic lines, and 80% for CN Violet. Uncertainties in flowfield kinetics and non-Boltzmann modeling provide the dominant contribution to these uncertainties. For the atomic nitrogen and oxygen lines, com- parisons between the nominal simulations and measurements show agreement well within these simulation uncertainty bounds, with the measurements within 20% of the nominal simulation over most of the trajectory, and within 10% at peak emission. This excellent agreement is un- precedented for observed radiation measurements of atomic lines, where previous Stardust and Hayabusa 1 comparisons were significantly worse. The improved agreement for these Hayabusa 2 comparisons is both the result of improved measurement quality and enhanced flowfield and radiation modeling. For the ablation product emission from the 656 nm atomic hydrogen line, the agreement between measurements and simulations is within ±20% for all trajectory points except one outlier. This good comparison is surprising considering this species depends on the ablation rate and is shown to have strong wake contribution, which both contribute to the large ≈±70% parametric uncertainty. For the ablation product emission from CN Violet, the simulations over-predict the measurements by over a factor of two early in the trajectory, with the comparison improving later in the trajectory. This disagreement for CN Violet is consistent with the ≈±80% parametric uncertainty bounds evaluated for the simulations. To utilize the excellent agreement between the atomic line simulations and measurements in the assessment of the radiative heating margin for a future flight vehicle, the flowfield property binning (FPB) approach is applied. Using the Mars Sample Return (MSR) Earth Entry System (EES) as an example, the combination of the excellent Hayabusa comparisons and the FPB analysis justify nearly a 10% reduction in the radiative heating margin from an assumed baseline margin of 30%. This direct quantitative link between observed radiation measurements and the radia- tive heating margin of future flight vehicles demonstrates the value of these observed radiation measurements.

Shock-Layer Radiative Heating↗

The Network for the Detection of Atmospheric Composition Change (NDACC): History, Status and Perspectives

The Network for the Detection of Atmospheric Composition Change (NDACC) is an international global network of more than 90 stations making high-quality measurements of atmospheric composition that began official operations in 1991 after 5 years of planning. Apart from sonde measurements, all measurements in the network are performed by ground-based remote-sensing techniques. Originally named the Network for the Detection of Stratospheric Change (NDSC), the name of the network was changed to NDACC in 2005 to better reflect the expanded scope of its measurements. The primary goal of NDACC is to establish long-term databases for detecting changes and trends in the chemical and physical state of the atmosphere (mesosphere, stratosphere, and troposphere) and to assess the coupling of such changes with climate and air quality. NDACC's origins, station locations, organizational structure, and data archiving are described. NDACC is structured around categories of ground-based observational techniques (sonde, lidar, microwave radiometers, Fourier-transform infrared, UV-visible DOAS (differential optical absorption spectroscopy)-type, and Dobson-Brewer spectrometers, as well as spectral UV radiometers), timely cross-cutting themes (ozone, water vapour, measurement strategies, cross-network data integration), satellite measurement systems, and theory and analyses. Participation in NDACC requires compliance with strict measurement and data protocols to ensure that the network data are of high and consistent quality. To widen its scope, NDACC has established formal collaborative agreements with eight other cooperating networks and Global Atmosphere Watch (GAW). A brief history is provided, major accomplishments of NDACC during its first 25 years of operation are reviewed, and a forward-looking perspective is presented.

lidar↗

Statistical Behavior of Low-Amplitude Power System Point-on-Wave Measurements

The power grid is undergoing massive changes to ensure resiliency and reliability in a more decentralized world. Distributed energy resources are becoming a prominent source of generation, potentially leading to a lack of centralized generation sources. Due to these new behaviors and system topologies, it is important to install measurement devices that are 1) accurate and 2) self-aware of their measurement quality. In this paper, a residential-scale microgrid is used to generate voltage and current waveforms, captured by Verivolt and National Instruments measurement equipment. A least-squares approach is used to separate the “clean” signals from the noise. Finally, Gaussian mixture modeling is used to approximate noise distributions, and it is shown these higher-order distribution estimates are a better fit to voltage and current noise profiles than single-mode Gaussian estimates.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Thermal Design Challenges for In-Flight Exposure to an Electric Propulsion Plasma Plume Environment

The use of electric propulsion to carry out NASA in-space propulsion demands has been increasing. A big part of fulfilling this demand is the Power and Propulsion Element (PPE) for NASA's Lunar Gateway. When built, the PPE will have the largest electric propulsion system to ever fly on a spacecraft, which brings new and difficult challenges. The environment created by the electric propulsion system during on-orbit operation of the thrusters has been shown to be different than those measured during operation in terrestrial vacuum facilities. Understanding the on-orbit environment created by the thrusters and its impacts on the spacecraft is the goal of the Plasma Diagnostic Package (PDP). The PDP is a sensor package, which is being developed by NASA GRC (Glenn Research Center), to fly on the PPE. The PDP will measure different aspects of the thruster plume in order to develop higher fidelity modeling of EP (Electric Power) systems. In order to capture quality measurements of the plume the PDP will need to install sensors in close proximity of it. This poses several unique thermal design challenges. Some of these challenges include: the long duration exposure to plume induced heating, the effects of plume induced erosion and sputter deposition on thermal control surfaces, and the extreme environments of a cis-lunar orbit. This paper looks to define the thermal challenges, explain modeling techniques, and offer design solutions for unique challenges of the PDP mission.

Thermal↗

Full-scale aerodynamic characteristics of a propellar installed on a small twin-engine aircraft wing panel

Full-scale measurements of shaft thrust and torque were made. Wind-tunnel speeds and blade angles were set for full-scale flight conditions. Excellent quality measurements were obtained of the thrust coefficient, the power coefficient, and the propeller efficiency for various values of the advance ratio and the blade incidence angle at 3/4-blade radius. A conventional propeller theory found in the literature was applied to the present results. Although thrust, power, and efficiency were somewhat overpredicted, the advance ratio for maximum efficiency was predicted quite accurately. It was found that, for some conditions, spinner drag could be significant. A simple correction that was based on the spinner base pressure substantially accounted for the changes in efficiency that resulted from this cause.

Barlow, P. R.↗

High Enthalpy Studies of Capsule Heating in an Expansion Tunnel Facility

Measurements were made on an Orion heat shield model to demonstrate the capability of the new LENS-XX expansion tunnel facility to make high quality measurements of heat transfer distributions at flow velocities from 3 km/s (h(sub 0) = 5 MJ/kg) to 8.4 km/s (h(sub 0) = 36 MJ/kg). Thirty-nine heat transfer gauges, including both thin-film and thermocouple instruments, as well as four pressure gauges, and high-speed Schlieren were used to assess the aerothermal environment on the capsule heat shield. Only results from laminar boundary layer runs are reported. A major finding of this test series is that the high enthalpy, low-density flows displayed surface heating behavior that is observed to be consistent with some finite-rate recombination process occurring on the surface of the model. It is too early to speculate on the nature of the mechanism, but the response of the gages on the surface seems generally repeatable and consistent for a range of conditions. This result is an important milestone in developing and proving a capability to make measurements in a ground test environment and extrapolate them to flight for conditions with extreme non-equilibrium effects. Additionally, no significant, isolated stagnation point augmentation ("bump") was observed in the tests in this facility. Cases at higher Reynolds number seemed to show the greatest amount of overall increase in heating on the windward side of the model, which may in part be due to small-scale particulate.

Dufrene, Aaron↗

Air Quality Science and Regulatory Efforts Require Geostationary Satellite Measurements

Air quality scientists and regulatory agencies would benefit from the high spatial and temporal resolution trace gas and aerosol data that could be provided by instruments on a geostationary platform. More detailed time-resolved data from a geostationary platform could be used in tracking regional transport and in evaluating mesoscale air quality model performance in terms of photochemical evolution throughout the day. The diurnal cycle of photochemical pollutants is currently missing from the data provided by the current generation of atmospheric chemistry satellites which provide only one measurement per day. Often peak surface ozone mixing ratios are reached much earlier in the day during major regional pollution episodes than during local episodes due to downward mixing of ozone that had been transported above the boundary layer overnight. The regional air quality models often do not simulate this downward mixing well enough and underestimate surface ozone in regional episodes. Having high time-resolution geostationary data will make it possible to determine the magnitude of this lower-and mid-tropospheric transport that contributes to peak eight-hour average ozone and 24-hour average PM2.5 concentrations. We will show ozone and PM(sub 2.5) episodes from the CMAQ model and suggest ways in which geostationary satellite data would improve air quality forecasting. Current regulatory modeling is typically being performed at 12 km horizontal resolution. State and regional air quality regulators in regions with complex topography and/or land-sea breezes are anxious to move to 4-km or finer resolution simulations. Geostationary data at these or finer resolutions will be useful in evaluating such models.

Pickering, Kenneth E.↗

Axion Dark Matter EXperiment 2A Cavity Characterization

The axion is a highly motivated dark matter candidate for its capacity to solve the strong CP problem in quantum chromodynamics. To detect the axion, the 2A phase of the Axion Dark Matter EXperiment (ADMX) utilizes four identical cylindrical resonant cavities that can detect the photons converted from axions in a strong magnetic field. We found the quality factors for various tunings in cavity D, and we also learned that the piezo motors are not currently equipped to handle cryogenic temperatures. However, the measured quality factors are much more promising than those shown in the last 2A cooldown, improving by about a factor of five.

Maglione, Diego↗