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Protein machines and self assembly in muscle organization

The remarkable order of striated muscle is the result of a complex series of protein interactions at different levels of organization. Within muscle, the thick filament and its major protein myosin are classical examples of functioning protein machines. Our understanding of the structure and assembly of thick filaments and their organization into the regular arrays of the A-band has recently been enhanced by the application of biochemical, genetic, and structural approaches. Detailed studies of the thick filament backbone have shown that the myosins are organized into a tubular structure. Additional protein machines and specific myosin rod sequences have been identified that play significant roles in thick filament structure, assembly, and organization. These include intrinsic filament components, cross-linking molecules of the M-band and constituents of the membrane-cytoskeleton system. Muscle organization is directed by the multistep actions of protein machines that take advantage of well-established self-assembly relationships. Copyright 1999 John Wiley & Sons, Inc.

NASA Discipline Musculoskeletal

Determinants of the mechanical properties of bones

The mechanical properties of bones are governed by the same principles as those of man-made load-bearing structures, but the organism is able to adapt its bone structure to changes in skeletal loading. In this overview of the determinants of the strength and stiffness of bone, a continuum approach has been taken, in which the behavior of a macroscopic structure depends on its shape and size, and on the mechanical properties of the material within. The latter are assumed to depend on the composition (porosity and mineralization) and organization (trabecular or cortical bone architecture, collagen fiber orientation, fatigue damage) of the bone. The effects of each of these factors are reviewed. Also, the possible means of non-invasively estimating the strength or other mechanical properties of a bone are reviewed, including quantitative computed tomography, photon absorptiometry, and ultrasonic measurements. The best estimates of strength have been obtained with photon absorptiometry and computed tomography, which at best are capable of accounting for 90% of the strength variability in a simple in vitro test, but results from different laboratories have been highly variable.

Non-NASA Center

SOHO Ultraviolet Coronagraph Spectrometer (UVCS) Mission Operations and Data Analysis

The scientific goal of UVCS is to obtain detailed empirical descriptions of the extended solar corona as it evolves from a period of minimum activity to maximum and to use these descriptions to identify and understand the physical processes responsible for coronal heating, solar wind acceleration, coronal mass ejections (CME's), and the phenomena that establish the plasma properties of the solar wind as measured by the CELIAS/SOHO experiment. We must observe a wide range of conditions (e.g., heating rate, impulsive mass ejections, magnetic field opening) in order to choose among competing theoretical explanations of the observed coronal structures and dynamics. During the first year of scientific observations (1 April 1996 through 31 March 1997), the LTVCS made the first extensive ultraviolet spectroscopic observations above the base of the solar corona. Those observations revealed extremely high most probable speeds along the line of sight for O(5+) in polar coronal holes above 2 solar radii from sun center and smaller such speeds for H(0) and protons. Doppler dimming and pumping diagnostics indicated that O(5+) and H(0) I reached supersonic outflow velocities within 2 solar radii. The observations appeared to be consistent with O95+) heating and direct momentum transfer by high frequency MHD waves through ion cyclotron resonance. Equatorial streamers were found to appear much different when observed in O VI, Fe XII and Mg X than when observed in H I Lyman-alpha or visible light. This led G. Noci to suggest that the elemental abundance of O, Fe, and Mg are lower in the dark center region of the O VI streamer image than it is in the brighter outer legs of the streamer. The O VI line ratio indicated a much smaller outflow speed in streamers than in the surrounding coronal holes. Spectroscopic observations of CME's in June and December 1996 showed large Doppler shifts indicating macroscopic flows of up to 200 km/s transverse to the apparent motion of the CME. The December event displayed factor of 500 intensity increases in H I Lyman lines and bright emissions in cool ion lines like C III 977 A indicating that the origin of some of the gas was a prominence. UVCS also observed two comets, several stars and interplanetary hydrogen. In the current reporting period, the initial results were published and detailed analyses including self consistent empirical models were developed. The research results were reported, published or submitted/accepted for publication. New observations were made to uncover additional information about the surprising results of the first year and other new observations revealed more surprises. A UVCS Tutorial was developed to familiarize potential users of UVCS data with the data products, analysis techniques and coronal models needed to analyze the data.

Kohl, John L.

Cyber-Physical System: Design for Sustainability and Resilience

When considering the design tools needed in the transition from numeric models to pilot plant, cyber-physical systems (CPS) come to the forefront as a method to model complex integrated energy systems. CPS approach has proven to be valuable to identify opportunities for economically viable early adoption of integrated energy technologies. This tutorial will introduce the concepts and the roles of CPS in co-design to minimize risks for pilot plant and technology deployment. This tutorial will also layout basic requirements for the CPS development, which requires a highly interdisciplinary effort with expertise in sensors, hardware testing, real-time modeling, controls, and system integration.

Harun, Nor Farida

Techniques for modeling the reliability of fault-tolerant systems with the Markov state-space approach

This paper presents a step-by-step tutorial of the methods and the tools that were used for the reliability analysis of fault-tolerant systems. The approach used in this paper is the Markov (or semi-Markov) state-space method. The paper is intended for design engineers with a basic understanding of computer architecture and fault tolerance, but little knowledge of reliability modeling. The representation of architectural features in mathematical models is emphasized. This paper does not present details of the mathematical solution of complex reliability models. Instead, it describes the use of several recently developed computer programs SURE, ASSIST, STEM, and PAWS that automate the generation and the solution of these models.

Butler, Ricky W.

SISO Space Reference FOM 101

This is a tutorial that covers the basic concepts associated with the SISO Space Reference Federation Object Model. It will be given at the SISO 2020 SIW in Orlando, Florida.

Crues, Edwin Zack

Quantum simulation of boson-related Hamiltonians: techniques, effective Hamiltonian construction, and error analysis

Elementary quantum mechanics proposes that a closed physical system consistently evolves in a reversible manner. However, control and readout necessitate the coupling of the quantum system to the external environment, subjecting it to relaxation and decoherence. Consequently, system-environment interactions are indispensable for simulating physically significant theories. A broad spectrum of physical systems in condensed-matter and high-energy physics, vibrational spectroscopy, and circuit and cavity QED necessitates the incorporation of bosonic degrees of freedom, such as phonons, photons, and gluons, into optimized fermion algorithms for near-future quantum simulations. In particular, when a quantum system is surrounded by an external environment, its basic physics can usually be simplified to a spin or fermionic system interacting with bosonic modes. Nevertheless, troublesome factors such as the magnitude of the bosonic degrees of freedom typically complicate the direct quantum simulation of these interacting models, necessitating the consideration of a comprehensive plan. This strategy should specifically include a suitable fermion/boson-to-qubit mapping scheme to encode sufficiently large yet manageable bosonic modes, and a method for truncating and/or downfolding the Hamiltonian to the defined subspace for performing an approximate but highly accurate simulation, guided by rigorous error analysis. In this pedagogical tutorial review, we aim to provide such an exhaustive strategy, focusing on encoding and simulating certain bosonic-related model Hamiltonians, inclusive of their static properties and time evolutions. Specifically, we emphasize two aspects: (1) the discussion of recently developed quantum algorithms for these interacting models and the construction of effective Hamiltonians, and (2) a detailed analysis regarding a tightened error bound for truncating the bosonic modes for a class of fermion-boson interacting Hamiltonians.

bosonic Hamiltonian

Waveform Simulation Framework: User Manual with Tutorials

This manuscript describes the Waveform Simulation Framework (WSF), a Python-based framework that provides a unified, programmable interface for generating synthetic seismograms for applications such as seismic array design, method development, and special event analysis. WSF standardizes how users define sources, receivers, and velocity models while abstracting simulator-specific configuration details, enabling workflows that are largely independent of the underlying numerical engine. The document provides installation guidance and tutorial-driven examples for three WSF simulator wrappers—WSF PyFK, WSF SW4, and WSF SPECFEM2D—illustrating end-to-end workflows from forward waveform simulation to common post-processing tasks (e.g., visualization and backprojection) using consistent data products (e.g., ObsPy Stream objects and SAC files).

97 MATHEMATICS AND COMPUTING

Motion models in attitude estimation

Attitude estimator use observations from different times to reduce the effects of noise. If the vehicle is rotating, the attitude at one time needs to be propagated to that at another time. If the vehicle measures its angular velocity, attitude propagating entails integrating a rotational kinematics equation only. If a measured angular velocity is not available, torques can be computed and an additional rotational dynamics equation integrated to give the angular velocity. Initial conditions for either of these integrations come from the estimation process. Sometimes additional quantities, such as gyro and torque parameters, are also solved for. Although the partial derivatives of attitude with respect to initial attitude and gyro parameters are well known, the corresponding partial derivatives with respect to initial angular velocity and torque parameters are less familiar. They can be derived and computed numerically in a way that is analogous to that used for the initial attitude and gyro parameters. Previous papers have demonstrated the feasibility of using dynamics models for attitude estimation but have not provided details of how each angular velocity and torque parameters can be estimated. This tutorial paper provides some of that detail, notably how to compute the state transition matrix when closed form expressions are not available. It also attempts to put dynamics estimation in perspective by showing the progression from constant to gyro-propagated to dynamics-propagated attitude motion models. Readers not already familiar with attitude estimation will find this paper an introduction to the subject, and attitude specialists may appreciate the collection of heretofore scattered results brought together in a single place.

Chu, D.

Understanding oblique impacts from experiments, observations, and modeling

Natural impacts in which the projectile strikes the target vertically are virtually nonexistent. Nevertheless, our inherent drive to simplify nature often causes us to suppose most impacts are nearly vertical. Recent theoretical, observational, and experimental work is improving this situation, but even with the current wealth of studies on impact cratering, the effect of impact angle on the final crater is not well understood. Although craters' rims may appear circular down to low impact angles, the distribution of ejecta around the crater is more sensitive to the angle of impact and currently serves as the best guide to obliquity of impacts. Experimental studies established that crater dimensions depend only on the vertical component of the impact velocity. The shock wave generated by the impact weakens with decreasing impact angle. As a result, melting and vaporization depend on impact angle; however, these processes do not seem to depend on the vertical component of the velocity alone. Finally, obliquity influences the fate of the projectile: in particular, the amount and velocity of ricochet are a strong function of impact angle.

NASA Discipline Exobiology

PrOMMiS Tutorial

This is a tutorial on using parameter estimation and process optimization in PrOMMiS. The tutorials are publicly available in the GitHub repository. The presentation attached guides through the flow of the three different tutorials. The tutorials are presented as follows: i) Parameter estimation of oxalate precipitation, ii) Optimization of precipitation model, and iii) Optimization of full process.

critical minerals and materials

Acceleration of energetic particles

Acceleration of some particles from the background plasma to highly superthermal energies is a common feature of collisionless shocks in interplanetary space. The complete in situ measurements of bulk flows, thermal plasma, electromagnetic waves and energetic particles available for interplanetary shocks both suggest and test the models of shock acceleration in the solar system and the rest of the universe. This tutorial will review the present state of shock acceleration theory. Scatter-free acceleration by grad B drift in the plasma electric field E = -V x B/c, and diffusive acceleration resulting from scattering by Alfven waves in the plasma on both sides of the shock are discussed. The diffusive theory in simple steady state gives power-law spectra, but also shows the effects of time-dependent shocks and injection rates, escape and other losses, the self-consistent Alfven wave spectra, and plasma flow fields affected by diffusive energetic particle acceleration.

Forman, M. A.

Role of PHM in Autonomous Decision-Making: Aerospace applications

There is an increased need for onboard decision-making capabilities in cyber-physical systems be it in energy, automotive, aviation, space, or other industries as they aim for increased efficiency, resiliency, and mission assurance capabilities. Emerging next-gen technologies such as multi-rover planetary missions, distributed satellites, unmanned ground and aerial vehicle operations and smart grid systems rely on in-time risk assessment and autonomous decision-making. One critical piece of the autonomy puzzle is reliable prediction of system behavior under time-varying and potentially uncertain environmental conditions. Further, if agent states change during operation such as initiation of faults or degradation, reliable diagnostic tools need to be investigated. In this tutorial, we will revise approaches that integrates existing physics-based and data-driven models of agents interacting with probability models of the environment and component operation state. Role of existing PHM methodologies as they feed into decision-making under uncertainty will be studied. Balancing critical trade-offs between high-fidelity prognostic models, prediction time-horizons and the computational requirements for in-time cost-effective decision-making will be discussed through the implementation of surrogate models. Finally, the audience will be introduced to a real-time application of in-time trajectory planning of an unmanned aerial system (UAS) based on its PHM assessments under uncertain and varying wind conditions.

decision-making

Optimizing Repowering and Lifecycle Decisions with PV ICE and SAM

Should you repower or extend the life of your PV system? Are high-efficiency modules, durable modules, or recyclable modules the best option for your site and goals? Evaluating the trade-offs in design and lifecycle strategies can be complex. The PV in Circular Economy (PV ICE) tool is an open-source model designed to help developers, modelers, and decision-makers assess material flows, energy return on investment (EROI), and financial viability of PV systems. Now integrated with the System Advisor Model (SAM), PV ICE enables site-specific comparisons of lifecycle strategies - such as repowering benefits, module selection for reliability and recyclability, among others. This interactive tutorial will provide hands-on experience with PV ICE using Google Collab, exploring scenario-based analyses on these topics.

36 MATERIALS SCIENCE

Overview of nuclear fragmentation models and needs

It has been known for some time that adequate assessment of spacecraft shield requirements and concomitant estimates of astronauts radiation exposures from galactic cosmic radiation requires accurate, quantitative methods for characterizing these radiation fields as they pass through thick absorbers. The main nuclear interaction processes involved are (1) nuclear elastic and inelastic collisions, and (2) nuclear breakup (fragmentation) and electromagnetic dissociation (EMD). Nuclear fragmentation and EMD are important because they alter the elemental and isotopic composition of the transported radiation fields. At present, there is no suitably accurate theory for predicting nuclear fragmentation cross sections for all collision pairs and energies of interest in space radiation protection. Typical cross-section differences between theory and experiment range from about 25 percent to a factor of two. The resulting errors in transported flux, for high linear energy transfer (LET) particles, are comparble to these cross-section errors. In this overview, theoretical models of heavy ion fragmentation currently used to generate input data bases for cosmic-ray transport and shielding codes are reviewed. Their shortcomings are discussed. Further actions needed to improve their accuracy and generality are presented.

Review

Considerations in miniaturizing simplified agro-ecosystems for advanced life support

Miniaturizing the Earth's biogeochemical cycles to support human life during future space missions is the goal of the NASA research and engineering program in advanced life support. Mission requirements to reduce mass, volume, and power have focused efforts on (1) a maximally simplified agro-ecosystem of humans, food crops, and microbes; and, (2) a design for optimized productivity of food crops with high light levels over long days, with hydroponics, with elevated carbon dioxide and other controlled environmental factors, as well as with genetic selection for desirable crop properties. Mathematical modeling contributes to the goals by establishing trade-offs, by analyzing the growth and development of experimental crops, and by pointing to the possibilities of directed phasic control using modified field crop models to increase the harvest index.

Non-NASA Center

AMMPER: a user-friendly agent-based model that recapitulates simple metabolic responses of yeast to deep-space radiation

For humans venturing to deep space, radiation exposure poses a major health risk. Fundamental research into the biological effects of space radiation are essential for enabling exploration, and the first experimental organisms we send to deep space will be microbial. Yet there are many ways in which microorganisms are likely to experience the effects of high-energy particle radiation (such as Galactic Cosmic Rays) differently from multicellular animals, partly due to the simple fact that microbes are small and unicellular-- less likely to get hit in the first place, and less likely to communicate damage between cells. Computational modeling can aid in designing experiments and predicting the biological effects of radiation, but thus far particle radiation models have not focused on microbes. Here we present the latest developments in AMMPER, the Agent-based Model for Microbial Populations Exposed to Radiation. Originally written in 2021, AMMPER is a Python-based model that incorporates radiation track data from NASA's RITRACKS software and simulates the growth, damage, and death of yeast cells in 3D. It is now freely available as an open-source package on NASA's GitHub repository. Recent improvements include the ability to simulate the dynamics of alamarBlue, a color-changing redox dye commonly used to track metabolic activity in microbial spaceflight experiments. We demonstrate that a simple blue-pink-clear transition model is able to recapitulate key features observed in empirical data from ground studies. AMMPER also includes a new graphical user interface and introductory tutorial to facilitate ease of use by a wider audience. AMMPER can help us to understand how spatially heterogeneous particle radiation damage at the single-cell level can translate to growth differences at the population level, ultimately allowing us to better interpret experiments using microbes as model organisms and how well their results apply to humans.

yeast