SEARCH · Search NASA

Results for “network robustness”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

SourceResearch connectionFrom yearThrough year

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 109 records · Page 6

All sources NASA NTRS DOE OSTI

NASA NTRSDate not supplied

QuantifyML: How Good is My Machine Learning Model?

We present QuantifyML, which applies model counting to assess the learn ability, safety, and robustness of machine learning models. Typically, the efficacy of machine learning models is determined by computing their accuracy statistically on test datasets. However, this may be misleading, if the test data is not representative of the problem that is being studied. With QuantifyML we aim to precisely quantify the extent to which machine learning models have learned and generalized from the given data. In QuantifyML, a trained model is translated into aC program, which is fed to the CBMC model checking tool to produce a formula in Conjunctive Normal Form (CNF), which in turn is analyzed with state-of-the-art model counters to efficiently obtain precise countsw.r.t different outputs. QuantifyML enables i) evaluating the learnability of models by comparing the counts for the outputs to ground truth, ex-pressed as logical predicates (if available), ii) comparing the performance of different models that may be built with different machine learning algorithms (e.g., decision-trees vs. neural networks), and iii) quantifying the safety and robustness of trained models.

Deep Neural Networks↗

NASA NTRS2004

Transportation Network Topologies

A discomforting reality has materialized on the transportation scene: our existing air and ground infrastructures will not scale to meet our nation's 21st century demands and expectations for mobility, commerce, safety, and security. The consequence of inaction is diminished quality of life and economic opportunity in the 21st century. Clearly, new thinking is required for transportation that can scale to meet to the realities of a networked, knowledge-based economy in which the value of time is a new coin of the realm. This paper proposes a framework, or topology, for thinking about the problem of scalability of the system of networks that comprise the aviation system. This framework highlights the role of integrated communication-navigation-surveillance systems in enabling scalability of future air transportation networks. Scalability, in this vein, is a goal of the recently formed Joint Planning and Development Office for the Next Generation Air Transportation System. New foundations for 21PstP thinking about air transportation are underpinned by several technological developments in the traditional aircraft disciplines as well as in communication, navigation, surveillance and information systems. Complexity science and modern network theory give rise to one of the technological developments of importance. Scale-free (i.e., scalable) networks represent a promising concept space for modeling airspace system architectures, and for assessing network performance in terms of scalability, efficiency, robustness, resilience, and other metrics. The paper offers an air transportation system topology as framework for transportation system innovation. Successful outcomes of innovation in air transportation could lay the foundations for new paradigms for aircraft and their operating capabilities, air transportation system architectures, and airspace architectures and procedural concepts. The topology proposed considers air transportation as a system of networks, within which strategies for scalability of the topology may be enabled by technologies and policies. In particular, the effects of scalable ICNS concepts are evaluated within this proposed topology. Alternative business models are appearing on the scene as the old centralized hub-and-spoke model reaches the limits of its scalability. These models include growth of point-to-point scheduled air transportation service (e.g., the RJ phenomenon and the 'Southwest Effect'). Another is a new business model for on-demand, widely distributed, air mobility in jet taxi services. The new businesses forming around this vision are targeting personal air mobility to virtually any of the thousands of origins and destinations throughout suburban, rural, and remote communities and regions. Such advancement in air mobility has many implications for requirements for airports, airspace, and consumers. These new paradigms could support scalable alternatives for the expansion of future air mobility to more consumers in more places.

Holmes, Bruce J.↗

NASA NTRS2004

Transportation Network Topologies

A discomforting reality has materialized on the transportation scene: our existing air and ground infrastructures will not scale to meet our nation's 21st century demands and expectations for mobility, commerce, safety, and security. The consequence of inaction is diminished quality of life and economic opportunity in the 21st century. Clearly, new thinking is required for transportation that can scale to meet to the realities of a networked, knowledge-based economy in which the value of time is a new coin of the realm. This paper proposes a framework, or topology, for thinking about the problem of scalability of the system of networks that comprise the aviation system. This framework highlights the role of integrated communication-navigation-surveillance systems in enabling scalability of future air transportation networks. Scalability, in this vein, is a goal of the recently formed Joint Planning and Development Office for the Next Generation Air Transportation System. New foundations for 21st thinking about air transportation are underpinned by several technological developments in the traditional aircraft disciplines as well as in communication, navigation, surveillance and information systems. Complexity science and modern network theory give rise to one of the technological developments of importance. Scale-free (i.e., scalable) networks represent a promising concept space for modeling airspace system architectures, and for assessing network performance in terms of scalability, efficiency, robustness, resilience, and other metrics. The paper offers an air transportation system topology as framework for transportation system innovation. Successful outcomes of innovation in air transportation could lay the foundations for new paradigms for aircraft and their operating capabilities, air transportation system architectures, and airspace architectures and procedural concepts. The topology proposed considers air transportation as a system of networks, within which strategies for scalability of the topology may be enabled by technologies and policies. In particular, the effects of scalable ICNS concepts are evaluated within this proposed topology. Alternative business models are appearing on the scene as the old centralized hub-and-spoke model reaches the limits of its scalability. These models include growth of point-to-point scheduled air transportation service (e.g., the RJ phenomenon and the Southwest Effect). Another is a new business model for on-demand, widely distributed, air mobility in jet taxi services. The new businesses forming around this vision are targeting personal air mobility to virtually any of the thousands of origins and destinations throughout suburban, rural, and remote communities and regions. Such advancement in air mobility has many implications for requirements for airports, airspace, and consumers. These new paradigms could support scalable alternatives for the expansion of future air mobility to more consumers in more places.

Holmes, Bruce J.↗

NASA NTRSDate not supplied

Case Study: Analysis of Autonomous Center line Tracking Neural Networks

Deep neural networks have gained widespread usage in a number of applications. However, limitations such as lack of explainability and robustness inhibit building trust in their behavior, which is crucial in safety critical applications such as autonomous driving. Therefore, techniques which aid in understanding and providing guarantees for neural network behavior are the need of the hour. In this paper, we present a case study applying a recently proposed technique, Prophecy, to analyze the behavior of a neural network model, provided by our industry partner and used for autonomous guiding of airplanes on taxi runways. This regression model takes as input an image of the runway and produces two outputs, cross-track error and heading error, which represent the position of the plane relative to the center line. We use the Prophecy tool to extract neuron activation patterns for the correctness and safety properties of the model. We show the use of these patterns to identify features of the input that explain correct and incorrect behavior. We also use the patterns to provide guarantees of consistent behavior. We explore a novel idea of using sequences of images (instead of single images) to obtain good explanations and identify regions of consistent behavior.

Deep Neural Networks↗

NASA NTRS2003

Integration of Online Parameter Identification and Neural Network for In-Flight Adaptive Control

An indirect adaptive system has been constructed for robust control of an aircraft with uncertain aerodynamic characteristics. This system consists of a multilayer perceptron pre-trained neural network, online stability and control derivative identification, a dynamic cell structure online learning neural network, and a model following control system based on the stochastic optimal feedforward and feedback technique. The pre-trained neural network and model following control system have been flight-tested, but the online parameter identification and online learning neural network are new additions used for in-flight adaptation of the control system model. A description of the modification and integration of these two stand-alone software packages into the complete system in preparation for initial flight tests is presented. Open-loop results using both simulation and flight data, as well as closed-loop performance of the complete system in a nonlinear, six-degree-of-freedom, flight validated simulation, are analyzed. Results show that this online learning system, in contrast to the nonlearning system, has the ability to adapt to changes in aerodynamic characteristics in a real-time, closed-loop, piloted simulation, resulting in improved flying qualities.

Hageman, Jacob↗

NASA NTRS2003

Integration of Online Parameter Identification and Neural Network for In-Flight Adaptive Control

Hageman, Jacob J.↗

NASA NTRS1994

Neural net controller for inlet pressure control of rocket engine testing

Many dynamic systems operate in select operating regions, each exhibiting characteristic modes of behavior. It is traditional to employ standard adjustable gain proportional-integral-derivative (PID) loops in such systems where no apriori model information is available. However, for controlling inlet pressure for rocket engine testing, problems in fine tuning, disturbance accommodation, and control gains for new profile operating regions (for research and development) are typically encountered. Because of the capability of capturing I/O peculiarities, using NETS, a back propagation trained neural network is specified. For select operating regions, the neural network controller is simulated to be as robust as the PID controller. For a comparative analysis, the higher order moment neural array (HOMNA) method is used to specify a second neural controller by extracting critical exemplars from the I/O data set. Furthermore, using the critical exemplars from the HOMNA method, a third neural controller is developed using NETS back propagation algorithm. All controllers are benchmarked against each other.

Trevino, Luis C.↗

NASA NTRS1990

Identification of the connections in biologically inspired neural networks

We developed an identification method to find the strength of the connections between neurons from their behavior in small biologically-inspired artificial neural networks. That is, given the network external inputs and the temporal firing pattern of the neurons, we can calculate a solution for the strengths of the connections between neurons and the initial neuron activations if a solution exists. The method determines directly if there is a solution to a particular neural network problem. No training of the network is required. It should be noted that this is a first pass at the solution of a difficult problem. The neuron and network models chosen are related to biology but do not contain all of its complexities, some of which we hope to add to the model in future work. A variety of new results have been obtained. First, the method has been tailored to produce connection weight matrix solutions for networks with important features of biological neural (bioneural) networks. Second, a computationally efficient method of finding a robust central solution has been developed. This later method also enables us to find the most consistent solution in the presence of noisy data. Prospects of applying our method to identify bioneural network connections are exciting because such connections are almost impossible to measure in the laboratory. Knowledge of such connections would facilitate an understanding of bioneural networks and would allow the construction of the electronic counterparts of bioneural networks on very large scale integrated (VLSI) circuits.

Demuth, H.↗

NASA NTRS1998

Feedback Linearized Aircraft Control Using Dynamic Cell Structure

A Dynamic Cell Structure (DCS ) Neural Network was developed which learns a topology representing network (TRN) of F-15 aircraft aerodynamic stability and control derivatives. The network is combined with a feedback linearized tracking controller to produce a robust control architecture capable of handling multiple accident and off-nominal flight scenarios. This paper describes network and its performance for accident scenarios including differential stabilator lock, soft sensor failure, control, stability derivative variation, and turbulence.

Jorgensen, C. C.↗

NASA NTRSDate not supplied

Localization of Ad-Hoc Lunar Constellations in Communication Failure Modes for Distributed Spacecraft Autonomy

As lunar missions increase in complexity inspired by NASA’s Artemis Program, they will require reliable and sufficient capability of the Position, Navigation, and Timing (PNT) system to support their scientific objectives. In addition, NASA's Commercial Lunar Payload Services (CLPS) program initiates the proliferation of public and private exploration partnerships using small satellites from commercial and private organizations, expanding traditionally confined low Earth orbit to be used for missions beyond geosynchronous orbit (Zucherman et al., 2022). Therefore, the Lunar PNT system is also required to provide navigation services compatible with the smaller platforms being sent by the public and private sectors, like CubeSats. However, traditional approaches to deep space missions’ navigation based on ground radio facilities have difficulties in providing sufficient support for the increasing number of users and communication at a distance from the Earth (Kaplev et al., 2022). In particular, the existing Lunar navigation technologies such as weak signal global positioning system (GPS) and deep space network (DSN) are not able to ensure operations of the upcoming small-scale Lunar missions due to their limitations in localization performance as well as capacity aspects. Another way to provide Lunar PNT service is to create a dedicated Lunar global navigation satellite system (GNSS) constellation, like GNSS systems on Earth. Space agencies like NASA, ESA, and JAXA are now developing the lunar communications relay and navigation systems (LCRNS) and Lunar navigation satellite systems (LNSS). In their systems, satellites will be deployed in moon orbits to provide the communication, positioning, navigation, and timing (CPNT) service at the lunar south pole region where the Artemis base camp will be expected (Murata et al., 2022). Meanwhile, common challenges considered in lunar PNT research arise from poor geometry of the terrestrial GNSS satellites when seen from the lunar user, highly perturbed lunar orbits, and limitations in power, size, and cost of the equipment on lunar satellites (Iiyama et al., 2023). It is also not clear if there will be enough Lunar users to support the cost and resources this would require as the Low-cost surface missions may not be able to support the large power, mass, and weight requirements that these navigation solutions entail (Niemoeller et al., 2022). As an alternative, existing Lunar science and exploration assets could be used to create a low-cost, autonomous, ad-hoc, and on-demand mission-centric Lunar PNT swarm capable of providing PNT services to these low-cost lunar missions (Hagenau et al., 2021). Introducing the non-dedicated and ad-hoc Lunar navigation constellation gives a way to provide PNT services on-demand. The non-dedicated swarm assets of Lunar constellations are designed to localize themselves with minimal interaction with Earth by adding cooperative autonomous localization to lunar missions, freeing up valuable bandwidth and ground segment resources. An autonomous localization of Lunar constellations is based on the concept of the decentralized PNT system with a distributed extended Kalman filter (DEKF) approach to state estimation for minimal onboard operating costs. In the distributed data processing algorithm, computation is broken down and assigned to each satellite, resulting in a considerably decreased computational amount while maintaining the accuracy of the orbit ephemeris and clock offsets as the result of centralized data processing (Wen et al., 2019). The DEKF requires spacecraft to perform two-way ranging operations with each other to communicate simultaneously, leveraging neighbor two-way intersatellite link (ISL) measurements such as pseudoranges to, and relative velocities between, visible satellites as sensor values (Frank et al., 2021). The Lunar autonomous PNT simulation (LAPS) demonstrated the feasibility of orbital asset localization among ad-hoc Lunar small-sat constellations based on the DEKF in Hagenau et al. (2021) and evaluated the matching algorithm proposed by Frank et al. (2021) in scheduling position estimation updates. In previous papers, all assets and measurements are assumed to be always available without consideration of the impact of intermittent and permanent communication failure. This study presents localization performance with increasing levels of network degradation for swarm assets and users to demonstrate the robustness of the decentralized Lunar PNT service in more realistic scenarios. Main issues arising from communication failure include spacecraft permanent or transient loss, antenna failures, message delays, etc. We tested four possible reasons for network degradation for 7 days in 21 satellites frozen with an altitude of 5500 km, evenly spaced around 3 circular, 40 inclination orbital planes where each spacecraft has two directional antennas. As anchor nodes with an independent estimate of their position are required in the DEKF approach, two ground nodes in each pole and one node in the gateway were implemented in the simulation. First, the most probable failure scenario involves the loss of a single spacecraft due to solar interference and technical malfunctions of the assets. Losing the availability of a single spacecraft means losing the two-way ISL measurement of the asset in the DEKF update. In order to provide the best possible quality of PNT service with limited time and resources, the distributed Lunar constellations must schedule the communication activities. The scheduler leverages mixed-integer linear programming (MILP) for the coordination and scheduling of the desired “as-needed” localization service (Niemoeller et al., 2022). We assume the scheduler has completely excluded the spacecraft information before the DEKF update in the failure scenario. When a random spacecraft has been turned off at a specific time, the robustness of the autonomous Lunar PNT system is evaluated. The simulation results give an 11.5% degradation in median position accuracy compared to the idealized performance excluding the asset loss. Second, a large number of assets may vanish due to major hardware problems or meteor strikes around the moon. A multiple spacecraft loss can degrade the localization performance very fast by losing the communication ability to do cross-plane measurements and in-plane measurements in a 3-plane constellation. When the matching-based scheduler is aware of ISL availability, we investigate a large number of in-plane and cross-plane asset vanishments both in close proximity and equally spaced throughout the orbital plane. According to the simulations, the loss of in-plane measurements gives 40.2% degradation while cross-plane measurements degrade 50.5% of asset localization performance among available assets. Therefore, it is concluded that cross-plane measurements are more important in improving the position estimation accuracy. Third, spacecraft failure information can be lost due to the internal message delay, resulting in the DEKF update scheduler to solve the matching problem with unavailable assets. The DEKF update cycle is comprised of network setup, communication, and computations where a global broadcast network and a 2-way ISL network setup take 6 minutes in total (Frank et al., 2021). Once the broadcast network successfully transmits and receives information, a random spacecraft may lose its availability right before solving the matching problem. This means the matching solution is no longer optimal, resulting in degradation in the localization performance. A numerical assessment shows the matching-based scheduler with knowing failure holds 11.5% of position accuracy degradation, whereas the scheduler without knowing failure gives 34% degraded localization performance without asset loss. Fourth, a transient loss of a single or multiple spacecraft may occur due to their antenna outages. After losing the two-way ISL availability for a few DEKF update cycles, the availability of spacecraft can easily be recovered as their states have been independently updated using measurements from anchor nodes. It is likely that the longer failure will result in worse localization performance. We have tested the transient failure of a random single asset for 30 min in the simulation, which is losing 3 update cycles in the DEKF system. From the simulation results, the position accuracy has been degraded to 4.84% which is better than the degraded localization performance of 11.5% from the permanent loss scenario among available assets. In conclusion, the autonomous Lunar PNT system based on the DEKF approach shows the ability to maintain resilience and robustness in the possible communication failure scenarios, ensuring that localization accuracy is preserved across various network degradation and outages. Future studies on investigating user localization performance near the South Pole and the broadcast network system will be continued in the following months.

Yeji Kim↗

NASA NTRS2011

Observability and Estimation of Distributed Space Systems via Local Information-Exchange Networks

Spacecraft formation flying involves the coordination of states among multiple spacecraft through relative sensing, inter-spacecraft communication, and control. Most existing formation-flying estimation algorithms can only be supported via highly centralized, all-to-all, static relative sensing. New algorithms are proposed that are scalable, modular, and robust to variations in the topology and link characteristics of the formation exchange network. These distributed algorithms rely on a local information exchange network, relaxing the assumptions on existing algorithms. Distributed space systems rely on a signal transmission network among multiple spacecraft for their operation. Control and coordination among multiple spacecraft in a formation is facilitated via a network of relative sensing and interspacecraft communications. Guidance, navigation, and control rely on the sensing network. This network becomes more complex the more spacecraft are added, or as mission requirements become more complex. The observability of a formation state was observed by a set of local observations from a particular node in the formation. Formation observability can be parameterized in terms of the matrices appearing in the formation dynamics and observation matrices. An agreement protocol was used as a mechanism for observing formation states from local measurements. An agreement protocol is essentially an unforced dynamic system whose trajectory is governed by the interconnection geometry and initial condition of each node, with a goal of reaching a common value of interest. The observability of the interconnected system depends on the geometry of the network, as well as the position of the observer relative to the topology. For the first time, critical GN&C (guidance, navigation, and control estimation) subsystems are synthesized by bringing the contribution of the spacecraft information-exchange network to the forefront of algorithmic analysis and design. The result is a formation estimation algorithm that is modular and robust to variations in the topology and link properties of the underlying formation network.

Fathpour, Nanaz↗

NASA NTRS1997

Direct Adaptive Aircraft Control Using Dynamic Cell Structure Neural Networks

A Dynamic Cell Structure (DCS) Neural Network was developed which learns topology representing networks (TRNS) of F-15 aircraft aerodynamic stability and control derivatives. The network is integrated into a direct adaptive tracking controller. The combination produces a robust adaptive architecture capable of handling multiple accident and off- nominal flight scenarios. This paper describes the DCS network and modifications to the parameter estimation procedure. The work represents one step towards an integrated real-time reconfiguration control architecture for rapid prototyping of new aircraft designs. Performance was evaluated using three off-line benchmarks and on-line nonlinear Virtual Reality simulation. Flight control was evaluated under scenarios including differential stabilator lock, soft sensor failure, control and stability derivative variations, and air turbulence.

Jorgensen, Charles C.↗

NASA NTRSDate not supplied

Advances in Modeling Solar System Internet Structures and their Data Flows

With an ever-increasing presence in space, there is also an increasing burden on existing communications infrastructure. We are heading towards an inflection point where the traditional approach of scheduled, single-path communications for space will no longer be viable. One answer is Delay Tolerant Networking (DTN), which takes the once disparate system of point-to-point links and unifies them in a networked architecture, thereby making communications more scalable. However, much work remains for discovering and harnessing the underlying theory of DTN. For example, in the terrestrial setting the interplay between routing domains is well-understood, however this is not the case in DTNs. In this paper, we build up the fundamental foundations of DTN, with an emphasis on modeling time varying networks and data flows across them, with examples of cross-domain routing in a DTN. A lofty goal of DTN is to enable the so-called Solar System Internet (SSI), which implies a standardized and robust suite of protocols. These protocols include routing across disconnected networks using store, carry, and forward mechanisms, which is necessary due to the disconnections, delays, and mobility intrinsic to space networks. Due to these factors, each of which generalize traditional networking, there is a deep and rich theory of DTNs. Here we build off of past successes to broaden this theory while striving to keep actionable results a goal for future implementations and operations. The approach includes modeling the unicast, broadcast, and multicast communications using the language of hypergraphs, which capture the geometric properties of such networked communications algebraically. Also inherent to these networks is their time-varying nature, particularly given mobility, and hence we also cultivate modeling techniques that respect this time dependence. This leads us to develop models using tools from category theory and algebraic geometry, which provide a language well-suited to describing synchronization and optimization over such networks. We also introduce and study a novel generalization of curvature applicable to time-evolving networks, which provides quantitative controls on diffusion processes on the network. Because an interplanetary network would feature links with propagation delays the preclude discovery (feedback) mechanisms, they will always feature a scheduled component. However, it is beneficial to support discovery where possible. While DTNs do not yet have strong definitions for their analogues of autonomous systems or network areas, we show how to join dynamic and schedule-based routing domains, using the language of sheaves, which marks progress towards such definitions. We conclude with a discussion of the progress made, as well as suggestions for future work.

Delay Tolerant Networking↗

NASA NTRS1992

Neural joint control for Space Shuttle Remote Manipulator System

Neural networks are being used to control a robot arm in a telerobotic operation. The concept uses neural networks for both joint and inverse kinematics in a robotic control application. An upper level neural network is trained to learn inverse kinematic mappings. The output, a trajectory, is then fed to the Decentralized Adaptive Joint Controllers. This neural network implementation has shown that the controlled arm recovers from unexpected payload changes while following the reference trajectory. The neural network-based decentralized joint controller is faster, more robust and efficient than conventional approaches. Implementations of this architecture are discussed that would relax assumptions about dynamics, obstacles, and heavy loads. This system is being developed to use with the Space Shuttle Remote Manipulator System.

Atkins, Mark A.↗

NASA NTRS1994

A Novel Approach to Noise-Filtering Based on a Gain-Scheduling Neural Network Architecture

A gain-scheduling neural network architecture is proposed to enhance the noise-filtering efficiency of feedforward neural networks, in terms of both nominal performance and robustness. The synergistic benefits of the proposed architecture are demonstrated and discussed in the context of the noise-filtering of signals that are typically encountered in aerospace control systems. The synthesis of such a gain-scheduled neurofiltering provides the robustness of linear filtering, while preserving the nominal performance advantage of conventional nonlinear neurofiltering. Quantitative performance and robustness evaluations are provided for the signal processing of pitch rate responses to typical pilot command inputs for a modern fighter aircraft model.

Troudet, T.↗

NASA NTRS1996

Verification and Validation of KBS with Neural Network Components

Artificial Neural Network (ANN) play an important role in developing robust Knowledge Based Systems (KBS). The ANN based components used in these systems learn to give appropriate predictions through training with correct input-output data patterns. Unlike traditional KBS that depends on a rule database and a production engine, the ANN based system mimics the decisions of an expert without specifically formulating the if-than type of rules. In fact, the ANNs demonstrate their superiority when such if-then type of rules are hard to generate by human expert. Verification of traditional knowledge based system is based on the proof of consistency and completeness of the rule knowledge base and correctness of the production engine.These techniques, however, can not be directly applied to ANN based components.In this position paper, we propose a verification and validation procedure for KBS with ANN based components. The essence of the procedure is to obtain an accurate system specification through incremental modification of the specifications using an ANN rule extraction algorithm.

Wen, Wu↗

NASA NTRS1999

Systems Engineering Design Via Experimental Operation Research: Complex Organizational Metric for Programmatic Risk Environments (COMPRE)

Unique and innovative graph theory, neural network, organizational modeling, and genetic algorithms are applied to the design and evolution of programmatic and organizational architectures. Graph theory representations of programs and organizations increase modeling capabilities and flexibility, while illuminating preferable programmatic/organizational design features. Treating programs and organizations as neural networks results in better system synthesis, and more robust data modeling. Organizational modeling using covariance structures enhances the determination of organizational risk factors. Genetic algorithms improve programmatic evolution characteristics, while shedding light on rulebase requirements for achieving specified technological readiness levels, given budget and schedule resources. This program of research improves the robustness and verifiability of systems synthesis tools, including the Complex Organizational Metric for Programmatic Risk Environments (COMPRE).

Mog, Robert A.↗

NASA NTRS2017

BioWires: Conductive DNA Nanowires in a Computationally-Optimized, Synthetic Biological Platform for Nanoelectronic Fabrication

DNA is an ideal template for a biological nanowire-it has a linear structure several atoms thick; it possesses addressable nucleobase geometry that can be precisely defined; and it is massively scalable into branched networks. Until now, the drawback of DNA as a conducting nanowire been, simply put, its low conductance. To address this deficiency, we extensively characterize a chemical variant of canonical DNA that exploits the affinity of natural cytosine bases for silver ions. We successfully construct chains of single silver ions inside double-stranded DNA, confirm the basic dC-Ag+-dC bond geometry and kinetics, and show length-tunability dependent on mismatch distribution, ion availability and enzyme activity. An analysis of the absorbance spectra of natural DNA and silver-binding, poly-cytosine DNA demonstrates the heightened thermostability of the ion chain and its resistance to aqueous stresses such as precipitation, dialysis and forced reduction. These chemically critical traits lend themselves to an increase in electrical conductivity of over an order of magnitude for 11-base silver-paired duplexes over natural strands when assayed by STM break junction. We further construct and implement a genetic pathway in the E. coli bacterium for the biosynthesis of highly ionizable DNA sequences. Toward future circuits, we construct a model of transcription network architectures to determine the most efficient and robust connectivity for cell-based fabrication, and we perform sequence optimization with a genetic algorithm to identify oligonucleotides robust to changes in the base-pairing energy landscape. We propose that this system will serve as a synthetic biological fabrication platform for more complex DNA nanotechnology and nanoelectronics with applications to deep space and low resource environments.

DNA↗