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DOE OSTI2024

Ovine Herpesvirus 2 Glycoprotein B Complementation Restores Infectivity to a Bovine Herpesvirus 4 gB-Null Mutant

Ovine herpesvirus 2 (OvHV-2) and bovine herpesvirus 4 (BoHV-4) are gamma herpesviruses that belong to the genera Macavirus and Rhadinovirus, respectively. As with all herpesviruses, both OvHV-2 and BoHV-4 express glycoprotein B (gB), which plays an essential role in the infection of host cells. In that context, it has been demonstrated that a BoHV-4 gB-null mutant is unable to infect host cells. In this study, we used homologous recombination to insert OvHV-2 ORF 8, encoding gB, into the BoHV-4 gB-null mutant genome, creating a chimeric BoHV-4 virus carrying and expressing OvHV-2 gB (BoHV-4∆gB/OvHV-2-gB) that was infectious and able to replicate in vitro. We then evaluated BoHV-4∆gB/OvHV-2-gB as a potential vaccine candidate for sheep-associated malignant catarrhal fever (SA-MCF), a fatal disease of ungulates caused by OvHV-2. Using rabbits as a laboratory model for MCF, we assessed the safety, immunogenicity, and efficacy of BoHV-4∆gB/OvHV-2-gB in an immunization/challenge trial. The results showed that while BoHV-4∆gB/OvHV-2-gB was safe and induced OvHV-2 gB-specific humoral immune responses, immunization conferred only 28.5% protection upon challenge with OvHV-2. Therefore, future studies should focus on alternative strategies to express OvHV-2 proteins to develop an effective vaccine against SA-MCF.

Moré, Daniela D. (ORCID:0000000237606757)↗

DOE OSTI2025

Ion-rich Acceleration during an Eruptive Flux Rope Event in a Multiple Null-point Configuration

We report on the γ-ray emission above 100 MeV from the GOES M3.3 flare SOL2012-06-03. The hard X-ray (HXR) and microwave emissions have typical time profiles with a fast rise to a well-defined peak followed by a slower decay. The >100 MeV emission during the prompt phase displayed a double-peaked temporal structure with the first peak following the HXR and microwaves, and the second one, about 3 times stronger, occurring 17 ± 2 s later. The time profiles seem to indicate two separate acceleration mechanisms at work, where the second γ-ray peak reveals a potentially pure or at least largely dominant ion acceleration. The Atmospheric Imaging Assembly imaging shows a bright elliptical ribbon and a transient brightening in the northwestern (NW) region. Nonlinear force-free extrapolations at the time of the impulsive peaks show closed field lines connecting the NW region to the southeastern part of the ribbon, and the magnetic topology revealed clusters of nulls. These observations suggest a spine-and-fan geometry, and based on these observations, we interpret the second γ-ray peak as being due to the predominant acceleration of ions in a region with multiple null points. The >100 MeV emission from this flare also exhibits a delayed phase with an exponential decay of roughly 350 s. We find that the delayed emission is consistent with ions being trapped in a closed flux tube with gradual escape via their loss cone to the chromosphere.

Pesce-Rollins, Melissa [Istituto Nazionale di Fisi↗

DOE OSTI2025

Tightening the thermodynamic uncertainty relations with null-entropy events: What we learn when nothing happens

Fluctuation theorems establish that thermodynamic processes at the microscale can occasionally result in negative entropy production. At the microscale, another distinct possibility becomes more likely: processes where no entropy is produced overall. In this work, we explore the constraints imposed by such null-entropy events on the fluctuations of thermodynamic currents. By incorporating the probability of null-entropy events, we obtain tighter bounds on finite-time thermodynamic uncertainty relations derived from fluctuation theorems. We validate this framework using an example of a qudit SWAP engine.

FOS: Physical sciences↗

NASA NTRS1974

Some magnetic null lines of astrophysical interest. II

A list of magnetic null lines from selected spectroscopic analyses is presented for possible use in detailed studies of magnetic Ap stars. The presented data represent an extension of the magnetic null lines whose usefulness Shore and Adelman (1974) have recently demonstrated for the study of the overall physical conditions prevailing in the atmospheres of peculiar A stars and for testing the consequences of the mechanism of selective elemental diffusion.

Adelman, S. J.↗

NASA NTRS1982

Design and tolerance analysis of two null corrector designs for the Space Telescope fine guidance aspheric collimating mirror

The collimating mirror within the Fine Guidance Subsystem of the Space Telescope's Pointing Control System is aspherized in order to correct the pupil aberration. A null corrector is needed to test the collimating mirror in autocollimation. Triplet and doublet null corrector designs are subjected to tolerance sensitivity analyses, and the doublet design is chosen despite its more restricted tolerances because of its compactness and simplicity.

Friedman, I.↗

NASA NTRS1984

Null tests for oblate spheroids

In most real cases requiring simple null optics, the optical path difference cannot be reduced to zero but can be kept at a fractional wavelength level so that interferometric data reduction can be used to account for the residual error. In other cases, computer-generated holograms may be used to obtain apparently straight fringes when the desired surface is obtained. Two examples, one involving an f/2.5 concave oblate spheroid and the other a Paul-Baker secondary, are examined. It is shown that although the null tests are not generally perfect, the residual error is small and the tests are simple.

Rodgers, J. M.↗

NASA NTRS1985

Petrology of the Cangas de Onis and nulles regolith breccias Implications for parent body history

The present study of the Cangas de Onis and Nulles H chondrite regolith breccias indicates that the minerals in the matrices and equilibrated clasts have essentially the same compositional distributions, so that much of the material in the castic matrix would have to have been derived from the impact comminution of clats. The apparently exclusive occurrence of H6 clasts in Cangas de Onis, and H4 clasts in Nulles, suggests that, at the locations where these breccias formed, the regolith predominantly consisted of H6 and H4 material, respectively.

Williams, C. V.↗

NASA NTRS1987

Antimultipath communication by injecting tone into null in signal spectrum

A transmitter for digital radio communication creates a null by balanced encoding of data modulated on an RF carrier, and inserts a calibration tone within the null. This is accomplished by having the calibration tone coincide in phase and frequency with the transmitted radio frequency output, for coherent demodulation of data at the receiver where the tone calibration signal is extracted and used for multipath fading compensation.

Davarian, Faramaz↗

NASA NTRS1993

Another Nulling Hall-Effect Current-Measuring Circuit

Lightweight, low-power circuit provides noncontact measurement of alternating or direct current of many ampheres in main conductor. Advantages of circuit over other nulling Hall-effect current-measuring circuits is stability and accuracy increased by putting both analog-to-digital and digital-to-analog converters in nulling feedback loop. Converters and rest of circuit designed for operation at sampling rate of 100 kHz, but rate changed to alter time or frequency response of circuit.

Thibodeau, Phillip E.↗

NASA NTRS1992

Three-dimensional kinematic reconnection of plasmoids with nulls

The global nonlinear dynamics of magnetic field lines in plasmoids with a pair of nulls, where B = 0, is studied. The aim of this analysis is to describe the separatrix surfaces on which singularities can occur in ideal magnetohydrodynamics because of topological changes in the field. These separatrix surfaces should locate the boundary layers associated with 3D reconnection in the presence of resistivity or inertia. It is found that the field lines exhibit chaotic scattering with several properties in common with plasmoid models without nulls (in which one component of the magnetic field never changes sign). In particular, the singular surfaces can be fractal, implying complex current density structures down to the dissipation scale. These generic features are expected to exist in typical coronal magnetic geometries exhibiting three-dimensional reconnection and the formation of current sheets.

Lau, Yun-Tung↗

NASA NTRS2003

Radiant Temperature Nulling Radiometer

A self-calibrating nulling radiometer for non-contact temperature measurement of an object, such as a body of water, employs a black body source as a temperature reference, an optomechanical mechanism, e.g., a chopper, to switch back and forth between measuring the temperature of the black body source and that of a test source, and an infrared detection technique. The radiometer functions by measuring radiance of both the test and the reference black body sources; adjusting the temperature of the reference black body so that its radiance is equivalent to the test source; and, measuring the temperature of the reference black body at this point using a precision contact-type temperature sensor, to determine the radiative temperature of the test source. The radiation from both sources is detected by an infrared detector that converts the detected radiation to an electrical signal that is fed with a chopper reference signal to an error signal generator, such as a synchronous detector, that creates a precision rectified signal that is approximately proportional to the difference between the temperature of the reference black body and that of the test infrared source. This error signal is then used in a feedback loop to adjust the reference black body temperature until it equals that of the test source, at which point the error signal is nulled to zero. The chopper mechanism operates at one or more Hertz allowing minimization of l/f noise. It also provides pure chopping between the black body and the test source and allows continuous measurements.

Robert Ryan↗

NASA NTRS2002

Radiant Temperature Nulling Radiometer

A nulling, self-calibrating infrared radiometer is being developed for use in noncontact measurement of temperature in any of a variety of industrial and scientific applications. This instrument is expected to be especially well-suited to measurement of ambient or near-ambient temperature and, even more specifically, for measuring the surface temperature of a natural body of water. Although this radiometer would utilize the long-wavelength infrared (LWIR) portion of the spectrum (wavelengths of 8 to 12 m), its basic principle of operation could also be applied to other spectral bands (corresponding to other temperature ranges) in which the atmosphere is transparent and in which design requirements for sensitivity and temperature-measurement accuracy could be satisfied. The underlying principle of nulling and self-calibration is the same as that of a typical microwave radiometer, but because of differences between the characteristics of signals in the infrared and microwave spectral regions, the principle must be implemented in a different way. A detailed description of the instrument including an infrared photodetector equipped with focusing input optics [e.g., lens(es) and/or mirrors] and an input LWIR band-pass filter is presented.

Source record↗

NASA NTRS2002

Biological and genetic properties of the p53 null preneoplastic mammary epithelium

The absence of the tumor suppressor gene p53 confers an increased tumorigenic risk for mammary epithelial cells. In this report, we describe the biological and genetic properties of the p53 null preneoplastic mouse mammary epithelium in a p53 wild-type environment. Mammary epithelium from p53 null mice was transplanted serially into the cleared mammary fat pads of p53 wild-type BALB/c female to develop stable outgrowth lines. The outgrowth lines were transplanted for 10 generations. The outgrowths were ductal in morphology and progressed through ductal hyperplasia and ductal carcinoma in situ before invasive cancer. The preneoplastic outgrowth lines were immortal and exhibited activated telomerase activity. They are estrogen and progesterone receptor-positive, and aneuploid, and had various levels of tumorigenic potential. The biological and genetic properties of these lines are distinct from those found in most hyperplastic alveolar outgrowth lines, the form of mammary preneoplasia occurring in most traditional models of murine mammary tumorigenesis. These results indicate that the preneoplastic cell populations found in this genetically engineered model are similar in biological properties to a subset of precurser lesions found in human breast cancer and provide a unique model to identify secondary events critical for tumorigenicity and invasiveness.

NASA Discipline Radiation Health↗

NASA NTRS1994

Detection of Fatigue Cracks at Rivets with Self-Nulling Probe

A new eddy current probe developed at NASA Langley Research Center has been used to detect small cracks at rivets in aircraft lap splices [1]. The device has earlier been used to detect isolated fatigue cracks with a minimum detectable flaw size of roughly 1/2 to 1/3 the diameter of the probe [2]. The present work shows that the detectable flaw size for cracks originating at rivets can be greatly improved upon from that of isolated flaws. The use of a rotating probe method combined with spatial filtering has been used to detect 0.18 cm EDM notches, as measured from the rivet shank, with a 1.27 cm diameter probe and to detect flaws buried under the rivet head, down to a length of 0.076 cm, using a 0.32 cm diameter probe. The Self-Nulling Electromagnetic Flaw Detector induces a high density eddy current ring in the sample under test. A ferromagnetic flux focusing lens is incorporated such that in the absence of any inhomogeneities in the material under test only a minimal magnetic field will reach the interior of the probe. A magnetometer (pickup coil) located in the center of the probe therefore registers a null voltage in the absence of material defects. When a fatigue crack or other discontinuity is present in the test article the path of the eddy currents in the material is changed. The magnetic field associated with these eddy currents then enter into the interior of the probe, producing a large output voltage across the pickup coil leads. Further

Wincheski, Buzz↗

NASA NTRS2008

Extrasolar Planetary Imaging Coronagraph: Visible Nulling Coronagraph Testbed Results

The Extrasolar Planetary Imaging Coronagraph (EPIC) is a proposed NASA Discovery mission to image and characterize extrasolar giant planets in orbits with semi-major axes between 2 and 10 AU. EPIC will provide insights into the physical nature of a variety of planets in other solar systems complimenting radial velocity (RV) and astrometric planet searches. It will detect and characterize the atmospheres of planets identified by radial velocity surveys, determine orbital inclinations and masses, characterize the atmospheres around A and F stars, observed the inner spatial structure and colors of inner Spitzer selected debris disks. EPIC would be launched to heliocentric Earth trailing drift-away orbit, with a 3-year mission lifetime ( 5 year goal) and will revisit planets at least three times at intervals of 9 months. The starlight suppression approach consists of a visible nulling coronagraph (VNC) that enables high order starlight suppression in broadband light. To demonstrate the VNC approach and advance it's technology readiness the NASA Goddard Space Flight Center and Lockheed-Martin have developed a laboratory VNC and have demonstrated white light nulling. We will discuss our ongoing VNC work and show the latest results from the VNC testbed,

Lyon, Richard G.↗

NASA NTRS2007

Testing of the Mirrors for the Constellation-X Spectroscopy X-ray Telescope with a Refractive Null

We present an introduction to the use of a refractive null lens for testing grazing incidence x-ray mirrors for the Constellation-X mission. The singular role of mirror mounting in glass shell mirror metrology is also touched upon. We compare results achieved to date with mission requirements along with some of the unique properties of the null lens. Additionally, uses beyond mirror metrology are briefly discussed.

Lehan, John↗

NASA NTRS2009

Visible Nulling Coronagraph Testbed Results

The Extrasolar Planetary Imaging Coronagraph (EPIC) is a NASA Astrophysics Strategic Mission Concept study and a proposed NASA Discovery mission to image and characterize extrasolar giant planets in orbits with semi-major axes between 2 and 10 AU. EPIC would provide insights into the physical nature of a variety of planets in other solar systems complimenting radial velocity (RV) and astrometric planet searches. It will detect and characterize the atmospheres of planets identified by radial velocity surveys, determine orbital inclinations and masses, characterize the atmospheres around A and F stars, observed the inner spatial structure and colors of inner Spitzer selected debris disks. EPIC would be launched to heliocentric Earth trailing drift-away orbit, with a 5-year mission lifetime. The starlight suppression approach consists of a visible nulling coronagraph (VNC) that enables starlight suppression in broadband light from 480-960 nm. To demonstrate the VNC approach and advance it's technology readiness we have developed a laboratory VNC and have demonstrated white light nulling. We will discuss our ongoing VNC work and show the latest results from the VNC testbed.

Lyon, Richard G.↗

NASA NTRS2009

Balloon Exoplanet Nulling Interferometer (BENI)

We evaluate the feasibility of using a balloon-borne nulling interferometer to detect and characterize exosolar planets and debris disks. The existing instrument consists of a 3-telescope Fizeau imaging interferometer with 3 fast steering mirrors and 3 delay lines operating at 800 Hz for closed-loop control of wavefront errors and fine pointing. A compact visible nulling interferometer is under development which when coupled to the imaging interferometer would in-principle allow deep suppression of starlight. We have conducted atmospheric simulations of the environment above 100,000 feet and believe balloons are a feasible path forward towards detection and characterization of a limited set of exoplanets and their debris disks. Herein we will discuss the BENI instrument, the balloon environment and the feasibility of such as mission.

Lyon, Richard G.↗