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Regional Geology Capstone Report for the Midwestern Regional Carbon Sequestration Partnership (Volume V)

The Midwest Regional Carbon Sequestration Partnership (MRCSP) was founded in 2003 as part of the U.S. Department of Energy’s (DOE’s) Regional Carbon Sequestration Partnership initiative. Since its founding, MRCSP has made significant strides toward making CCUS a viable option for states in the region. The public/private consortium, funded through the DOE Regional Carbon Sequestration Initiative, brings together nearly 40 industry partners and 10 states. Battelle, as the project lead, oversees research, development and operations and coordinates activities among the partners. The incremental, phased approach has built a valuable knowledge base for the industry and paved the way for commercial-scale adoption of CCUS technologies. From 2008 to 2020, MRCSP Phase III focused on the development of large-scale injection projects. This report is part of a series of reports prepared under the Midwestern Regional Carbon Sequestration Partnership (MRCSP) Phase III (Development Phase). These reports summarize and detail the findings of the work conducted under the Phase III project. The MRCSP regional geology Capstone report summarizes the regional characterization of geologic storage subtasks performed under MRCSP Phase III. The regional characterization work was completed by the state geological surveys of Delaware, Indiana, Kentucky, Maryland, New York, Ohio, Pennsylvania, and West Virginia along with Western Michigan University and Rutgers University. Building on the Phases I and II, the Phase III research was focused on key research topics identified in previous MRCSP efforts: [1] Regional Geologic Cross Sections for Potential Storage and Containment Zones; [2] Appalachian Basin: Enhanced Recovery Opportunities; [3] Michigan Basin: Assessment of Enhanced Oil Recovery Using Carbon Dioxide in Silurian Pinnacle Reefs; [4] Mid-Atlantic Coastal Plain and Adjacent Offshore Region: Characterization of Carbon Storage Targets; [5] Ordovician-Cambrian Units: Hierarchical Evaluation of Geologic Carbon Storage Resource Estimates; [6] Upper Silurian to Middle Devonian Strata of Ohio: Structural Characterization of Potential CO 2 Reservoirs and Adjacent Strata; [7] Triassic Rift Basins: Preliminary Study of Long-Term CO 2 Storage Potential Together, the regional characterization effort developed useful products to support project developers, policy makers and other stakeholders in the MRCSP region seeking to understand where potential storage exists relative to large stationary sources of CO 2 emissions.

01 COAL, LIGNITE, AND PEAT↗

Distributed Acoustic Sensing (DAS) Seismic Monitoring of CO 2 Injected for Enhanced Oil Recovery in Northern Michigan (Volume II.C)

The Midwest Regional Carbon Sequestration Partnership (MRCSP) was founded in 2003 as part of the U.S. Department of Energy’s (DOE’s) Regional Carbon Sequestration Partnership initiative. Since its founding, MRCSP has made significant strides toward making CCUS a viable option for states in the region. The public/private consortium, funded through the DOE Regional Carbon Sequestration Initiative, brings together nearly 40 industry partners and 10 states. Battelle, as the project lead, oversees research, development and operations and coordinates activities among the partners. The incremental, phased approach has built a valuable knowledge base for the industry and paved the way for commercial-scale adoption of CCUS technologies. From 2008 to 2020, MRCSP Phase III focused on the development of large-scale injection projects. This report is part of a series of reports prepared under the Midwestern Regional Carbon Sequestration Partnership (MRCSP) Phase III (Development Phase). These reports summarize and detail the findings of the work conducted under the Phase III project. This report describes the monitoring study conducted to assess the effectiveness of DAS (Distributed Acoustic Sensing) -based VSP (Vertical Seismic Profiling) technology for delineating CO 2 injected into the Silurian-age pinnacle reefs in northern Michigan, the host rocks for the MRCSP Phase III demonstration project. The DAS VSP study was conducted in the Chester 16 reef, one of several reefs in Otsego County Michigan that is operated by Core Energy, LLC of Traverse City, Michigan. Time-lapse DAS VSP was implemented at the Chester 16 reef to attempt to detect approximately 85,000 tonnes of CO 2 injected into the A-1 Carbonate and Brown Niagaran Formations. A baseline survey was conducted in February 2017 prior to injecting CO 2 and a repeat survey was conducted in August 2018. During the interim period between the baseline and repeat surveys, CO 2 was injected into the Chester 16 reef via the 6-16 injection well without production (withdrawal) of fluids from the reef. A grid of 181 source positions consisting of 44 vibrator positions, plus 137 dynamite shot locations, was used to give approximately continuous spatial coverage of the injection zone (A-1 Carbonate and upper Brown Niagaran) in the area between the two wells.

01 COAL, LIGNITE, AND PEAT↗

Direct visualization of degradation microcompartments at the ER membrane

To promote the biochemical reactions of life, cells can compartmentalize molecular interaction partners together within separated non–membrane-bound regions. It is unknown whether this strategy is used to facilitate protein degradation at specific locations within the cell. Leveraging in situ cryo-electron tomography to image the native molecular landscape of the unicellular alga Chlamydomonas reinhardtii , we discovered that the cytosolic protein degradation machinery is concentrated within ∼200-nm foci that contact specialized patches of endoplasmic reticulum (ER) membrane away from the ER–Golgi interface. These non–membrane-bound microcompartments exclude ribosomes and consist of a core of densely clustered 26S proteasomes surrounded by a loose cloud of Cdc48. Active proteasomes in the microcompartments directly engage with putative substrate at the ER membrane, a function canonically assigned to Cdc48. Live-cell fluorescence microscopy revealed that the proteasome clusters are dynamic, with frequent assembly and fusion events. We propose that the microcompartments perform ER-associated degradation, colocalizing the degradation machinery at specific ER hot spots to enable efficient protein quality control.

Science & Technology - Other Topics↗

Solar Ready Wisconsin

Through the Solar Ready Wisconsin project, the MREA was able to facilitate collaboration between Wisconsin’s leading technical college training programs, increase student work experience opportunities, and facilitate workforce training on high impact/high visibility PV installations in Wisconsin communities. The workforce development partnership offered credit-bearing advanced installation trainings at WI technical colleges, allowing students to participate in hands on installation training opportunities. Students also participated in internships with a network of PV contractors developed through MREA’s regional network of members, contracted instructors, and program partners.

14 SOLAR ENERGY↗

MRCI Task 5: Promoting Regional Technology Transfer Final Summary Report

The goal of the MRCI Task 5 – Promoting Regional Technology Transfer is to leverage existing and new relationships with critical CCUS stakeholders (national and international stakeholders include state geological surveys, universities, industrial partners and advisors, energy production and utilization companies, and NGOs) within the regional initiative and globally and become a key resource for CCUS information, acceptance, and development. Work completed under Task 5 is categorized through four actions: • Promote acceleration of CCUS deployment by providing general support for commercialization and technology transfer • Compile and communicate information from previous tasks to interested stakeholders • Engage with federal and state governments, industry consortia and NGOs • Engage with global institutions

CCUS,Midwest,Midwest Regional Carbon Initiative,Mi↗

Synthesis of H2 in dirty ice mantles by fast ion energy loss: New experimental results increase the relevance of this mechanism

Recent experimental results support the importance of H2 production in molecular clouds by cosmic ray bombardment of the mantles of grains. The formation of molecules different from those originally present in the irradiated layer can be explained by the production of molecular fragments induced by the release of energy if the impinging fast particle. One way of considering the process is in terms of a transiently hot cylinder, initially about 50 A in diameter, that exists around the track of an individual fast ion. Since ice has a relatively low thermal conductivity, energy lost by the ion in the ice layers remains confined around the track for time long enough to be thermalized. The hot cylinder increases in diameter and decreases in temperature on a time scale of 10(exp -11) to 10(exp -10) sec. Molecular fragments that are formed in this high temperature region acquire enough mobility to recombine with different partners, forming new molecules. A Monte Carlo simulation of the interaction between cosmic rays and grain mantles, at various depths in the core of a spherical molecular cloud, was performed. The simulation was continued until 40,000 ions had hit each grain of the type and size chosen. During the performed experiments thin icy films made of H2O and CD4 mixed in the gas phase and deposited on a cold finger at 9 K were irradiated with 1.5 MeV helium beams. Among synthesized molecules were found H2, HD, and D2.

Pirranello, Valero↗

Hybrid Turbine Electric Vehicle

Hybrid electric power trains may revolutionize today's ground passenger vehicles by significantly improving fuel economy and decreasing emissions. The NASA Lewis Research Center is working with industry, universities, and Government to develop and demonstrate a hybrid electric vehicle. Our partners include Bowling Green State University, the Cleveland Regional Transit Authority, Lincoln Electric Motor Division, the State of Ohio's Department of Development, and Teledyne Ryan Aeronautical. The vehicle will be a heavy class urban transit bus offering double the fuel economy of today's buses and emissions that are reduced to 1/10th of the Environmental Protection Agency's standards. At the heart of the vehicle's drive train is a natural-gas-fueled engine. Initially, a small automotive engine will be tested as a baseline. This will be followed by the introduction of an advanced gas turbine developed from an aircraft jet engine. The engine turns a high-speed generator, producing electricity. Power from both the generator and an onboard energy storage system is then provided to a variable-speed electric motor attached to the rear drive axle. An intelligent power-control system determines the most efficient operation of the engine and energy storage system.

Viterna, Larry A.↗

Serving Fisheries and Ocean Metadata to Communities Around the World

NASA's Global Change Master Directory (GCMD) assists the oceanographic community in the discovery, access, and sharing of scientific data by serving on-line fisheries and ocean metadata to users around the globe. As of January 2006, the directory holds more than 16,300 Earth Science data descriptions and over 1,300 services descriptions. Of these, nearly 4,000 unique ocean-related metadata records are available to the public, with many having direct links to the data. In 2005, the GCMD averaged over 5 million hits a month, with nearly a half million unique hosts for the year. Through the GCMD portal (http://gcmd.nasa.gov/), users can search vast and growing quantities of data and services using controlled keywords, free-text searches, or a combination of both. Users may now refine a search based on topic, location, instrument, platform, project, data center, spatial and temporal coverage, and data resolution for selected datasets. The directory also offers data holders a means to advertise and search their data through customized portals, which are subset views of the directory. The discovery metadata standard used is the Directory Interchange Format (DIF), adopted in 1988. This format has evolved to accommodate other national and international standards such as FGDC and IS019115. Users can submit metadata through easy-to-use online and offline authoring tools. The directory, which also serves as the International Directory Network (IDN), has been providing its services and sharing its experience and knowledge of metadata at the international, national, regional, and local level for many years. Active partners include the Committee on Earth Observation Satellites (CEOS), federal agencies (such as NASA, NOAA, and USGS), international agencies (such as IOC/IODE, UN, and JAXA) and organizations (such as ESIP, IOOS/DMAC, GOSIC, GLOBEC, OBIS, and GoMODP).

Meaux, Melanie F.↗

Montana Water Resources II: Enhancing a Moisture Index for Drought and Flood Monitoring in the Missouri River Basin

The Missouri River Basin is a major global breadbasket, containing large amounts of agricultural land. Recent devastating weather events have motivated regional organizations to dedicate efforts to drought and flood monitoring and early warning systems. The DEVELOP team partnered with the following organizations: Montana Climate Office, NOAA National Weather Service (NWS) Missouri Basin River Forecast Center, NOAA Regional Climate Services of the Central Region, NOAA Physical Sciences Laboratory, and the US Army Corps of Engineers' Missouri River Basin Water Management Division. The team collaborated with the partners in their efforts to monitor flood and drought by enhancing a composite moisture index (CMI) for the Missouri River Basin. The CMI leverages NASA Earth observations to derive snow cover data from the Terra Moderate Resolution Imaging Spectroradiometer (MODIS) mission and snow water equivalent and snowdepth datasets from the NOAA NWS National Operational Hydrologic Remote Sensing Center’s Snow Data Assimilation System (SNODAS). Building upon this framework, the team added groundwater storage data from the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On missions, soil moisture data from Soil Moisture Active Passive (SMAP), and United States Geological Survey in situ streamflow data to the CMI. To test the tool’s validity, the team compared the CMI results to historically extreme dry and extreme wet years, March 2017 and March 2019, respectively. The CMI accurately reflects both 2017 and 2019 climate conditions in the Missouri River Basin. The refined CMI enhances the understanding of antecedent soil moisture conditions and improves flood and drought forecasting in the Missouri River Basin before the growing season.

Chloe Schneider↗

Kansas City, Missouri, Streetlight Electric Vehicle Charging: Strategies and challenges for site selection of streetlight electric vehicle infrastructure in Kansas City, Missouri (Final Report)

Public streetlight charging, whether on streets in central business districts or residential areas, provides easy charging access for apartment residents and homeowners alike. While most electric vehicle (EV) drivers charge at home, they do so in garages or on driveways they own. For renters and residents of multifamily housing (MFH), however, this may not be an option. EVs have a lower cost of ownership compared to conventional vehicles, and a used EV may be an affordable option for a lower-income household. But without easy access to charging, even a low-cost used EV may not be an option for a prospective buyer. An affordable curbside charging network has the potential to expand EV adoption into neighborhoods that have to date seen minimal interest and uptake of the technology and associated charging infrastructure. Streetlight charging networks can provide an economical, scalable, and effective approach to providing equitable and convenient charging. Metropolitan Energy Center (MEC) is dedicated to the mission of creating resource efficiency, environmental health, and economic vitality in the Kansas City region and beyond. Since 1983, MEC has provided resources, outreach, and training to make alternative fuels and energy efficiency commonplace. MEC led a streetlight charging pilot project that installed limited EV charging infrastructure on the streetlight system in Kansas City, Missouri, to demonstrate and test the benefits of curbside charging for EVs at existing on-street parking locations. The project aimed to cost-effectively expand the charging network in Kansas City to support residential charging and provide infrastructure in one or more charging deserts throughout the city. This pilot evaluates the impact and overall success of streetlight charging based on community feedback, utilization of charging infrastructure, technical feasibility, and cost. The project has pursued a data- and community-driven site selection process designed to identify sites with high demand and high opportunity for EV charging. This project was funded by the U.S. Department of Energy (DOE) and awarded to MEC through a competitive proposal process. The novelty and complexity of this project required an organization that could facilitate collaboration across levels of government, community members, and industry partners. For the past 25 years, through Kansas City Regional Clean Cities, MEC has worked with numerous public and private fleets on a variety of projects to improve the environmental performance and efficiency of the regional vehicle fleet. To advance affordable, efficient, and clean transportation efforts, DOE Clean Cities and Communities coalitions create local networks of public and private sector stakeholders and engage communities. Rooted within their local communities, the coalitions serve as experts and ambassadors, bringing to bear the collective knowledge, experience, and practical know-how of the entire network from within DOE, its national laboratories, and diverse stakeholders in the field. MEC and its project partners made in-kind contributions to leverage federal dollars for the benefit of the Kansas City community. Findings from this project will help determine the best applications for streetlight charging technologies to maximize funding impact and serve community needs. The team evaluated locations based on expected charging demand, technical feasibility, safety considerations, and enhanced charging network siting needs. Throughout the project, the team gathered feedback and evaluated ways to make public charging for EVs available to all community members. The insights will help Kansas City and other communities streamline future efforts to support EV drivers through public charging in the city right-of-way. Furthermore, this project will inform citywide guidance for future installations. MEC is committed to a transparent and publicly accessible approach that encourages the collaborative evaluation of streetlight charging. The project has engaged the community to proactively identify and evaluate the benefits and impacts of streetlight charging. It was a priority for the project to ensure the benefits of this pilot are distributed equitably to all members of the Kansas City community and that new charging opportunities and associated resources are available in diverse neighborhoods across the city. The charging infrastructure supports an affordable curbside charging network that will enable more drivers to choose EVs and provide easy charging access for all community members interested in driving an EV. The community feedback received through this project informed future resources and opportunities to make EVs more accessible to all members of the Kansas City community. MEC worked with several community partners on this project, including Missouri University of Science and Technology (MST), Pennsylvania State University (Penn State), the National Renewable Energy Laboratory (NREL); the city of Kansas City, Missouri; Evergy; Black and McDonald (B&M); LilyPad EV; EVNoire; and Westside Housing Organization (WHO). Project partners contributed to the cost match required for DOE grants through capital expenditures, personnel, and other in-kind contributions. Detailed descriptions of project team organizations can be found in Appendix A. Project Partners. Analysts at NREL and MST/PennState developed site maps based on demand and equity considerations. MEC conducted outreach to community members to garner input on project design and site selection, and received approval from the Missouri Public Service Commission (PSC) for Evergy’s EV charging station ownership. MEC worked with all partners to gather additional siting criteria and developed a site selection evaluation checklist, and partners conducted site visits to proposed installation sites. Next, B&M, Evergy, and the city executed all site agreements, conducted site-specific engineering design, acquired associated permits, and issued notices to proceed site by site or in small batches. Finally, from January to April 2023, the project team installed 23 EV charging stations built on Kansas City’s streetlight system in six council districts. Evergy will own, operate, and monitor the stations for 10 years, sharing charging data with MEC for at least 1 year.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Developing Land Use Land Cover Maps for the Lower Mekong Basin to Aid Hydrologic Modeling and Basin Planning

This paper discusses research methodology to develop Land Use Land Cover (LULC) mapsfor the Lower Mekong Basin (LMB) for basin planning, using both MODIS and Landsat satellitedata. The 2010 MODIS MOD09 and MYD09 8-day reflectance data was processed into monthlyNDVI maps with the Time Series Product Tool software package and then used to classify regionallycommon forest and agricultural LULC types. Dry season circa 2010 Landsat top of atmosphere reflectance mosaics were classified to map locally common LULC types. Unsupervised ISODATAclustering was used to derive most LULC classifications. MODIS and Landsat classifications werecombined with GIS methods to derive final 250-m LULC maps for Sub-basins (SBs) 1–8 of the LMB.The SB 7 LULC map with 14 classes was assessed for accuracy. This assessment compared randomlocations for sampled types on the SB 7 LULC map to geospatial reference data such as Landsat RGBs,MODIS NDVI phenologic profiles, high resolution satellite data, and Mekong River Commissiondata (e.g., crop calendars). The SB 7 LULC map showed an overall agreement to reference data of~81%. By grouping three deciduous forest classes into one, the overall agreement improved to ~87%.The project enabled updated regional LULC maps that included more detailed agriculture LULCtypes. LULC maps were supplied to project partners to improve use of Soil andWater AssessmentTool for modeling hydrology and water use, plus enhance LMB water and disaster managementin a region vulnerable to flooding, droughts, and anthropogenic change as part of basin planningand assessment.

land use land cover mapping; SWAT hydrologic model↗

Assessing Urban Heat in the Cincinnati and Covington Area to Inform Local Decision-Makers and Environmental Justice Initiatives

The Urban Heat Island effect is a phenomenon characterized by urban areas experiencing temperatures that are, on average, warmer than surrounding suburban and rural regions. Urban Heat Islands are fueled by expansive impervious surfaces, vehicle emissions, and insufficient urban green space. Densely populated urban centers like the Cincinnati, Ohio and Covington, Kentucky area can experience negative health impacts due to the Urban Heat Island. NASA DEVELOP partnered with Groundwork USA and Groundwork Ohio River Valley to combine environmental education and outreach with technical capacity building in NASA Earth observations. The DEVELOP team used Landsat 5 TM and ISS ECOSTRESS to calculate daytime and nighttime land surface temperature anomalies. The team found that the Cincinnati and Covington area was 8.32°F warmer during the day and 4.97°F warmer at night compared to non-urban areas. The team also ran the Natural Capital Project InVEST Urban Cooling Model to map a heat mitigation index for the study area. The heat mitigation index models the cooling capacity of each pixel and the effect of green spaces on the pixel’s ability to mitigate heat. Results show which communities are most vulnerable to impacts of increased urban heat. The team also assessed alternative tree canopy scenarios with the InVEST model to better understand the effectiveness of potential heat mitigation strategies. Increasing tree canopy cover by 25% in urbanized land cover types was found to reduce mean temperature across the study area by 0.87°F. Combining these results with work from Groundwork’s Climate Safe Neighborhoods program highlights the relationship between extreme heat and historical-race based housing practices in the region. Results provide partners at Groundwork with refined methodologies to support future education and outreach, as well as increase future capacity to NASA Earth observations.

Celeste Gambino↗

Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding

PUF proteins are characterized by globular RNA-binding domains. They also interact with partner proteins that modulate their RNA-binding activities. Caenorhabditis elegans PUF protein fem-3 binding factor-2 (FBF-2) partners with intrinsically disordered Lateral Signaling Target-1 (LST-1) to regulate target mRNAs in germline stem cells. Here, we report that an intrinsically disordered region (IDR) at the C-terminus of FBF-2 autoinhibits its RNA-binding affinity by increasing the off rate for RNA binding. Moreover, the FBF-2 C-terminal region interacts with its globular RNA-binding domain at the same site where LST-1 binds. This intramolecular interaction restrains an electronegative cluster of amino acid residues near the 5' end of the bound RNA to inhibit RNA binding. LST-1 binding in place of the FBF-2 C-terminus therefore releases autoinhibition and increases RNA-binding affinity. This regulatory mechanism, driven by IDRs, provides a biochemical and biophysical explanation for the interdependence of FBF-2 and LST-1 in germline stem cell self-renewal.

59 BASIC BIOLOGICAL SCIENCES↗

Utilizing Satellite Based Observations and Physical Hydrological Modeling for Freshwater Ecosystem Health in the Lower Mekong River Basin

Freshwater availability is necessary to promote economic growth through agriculture, fisheries, transport, environmental health, and social equity.The National Aeronautics and Space Administration (NASA) and the Conservation International (CI) are partnering to use remote sensing Earth observations to improve regional efforts that assess natural resources for conservation and sustainable management. (Vollmer et al.,2018) have presented the social-ecological framework named the Freshwater Health Index (FHI), which takes account of the interplay between governance, stakeholders, freshwater ecosystems and the ecosystem services they provide.In this work, we develop decision support and making tools for natural resources conservation in the Lower Mekong by leveraging the FHI framework, multiple data products, and hydrological modeling capabilities (Mohammed et al., 2018). Modeling capabilities enable the integration of satellite-based daily gridded precipitation, air temperature, digital elevation model, soil characteristics, and land cover and land use information to simulate water flux framework.

Mohammed, Ibrahim N.↗

HIMALA: Climate Impacts on Glaciers, Snow, and Hydrology in the Himalayan Region

Glaciers are the largest reservoir of freshwater on Earth, supporting one third of the world s population. The Himalaya possess one of the largest resources of snow and ice, which act as a freshwater reservoir for more than 1.3 billion people. This article describes a new project called HIMALA, which focuses on utilizing satellite-based products for better understanding of hydrological processes of the river basins of the region. With support from the US Agency for International Development (USAID), the International Centre for Integrated Mountain Development (ICIMOD), together with its partners and member countries, has been working on the application of satellite-based rainfall estimates for flood prediction. The US National Aeronautics and Space Administration (NASA) partners are working with ICIMOD to incorporate snowmelt and glacier melt into a widely used hydrological model. Thus, through improved modeling of the contribution of snow and ice meltwater to river flow in the region, the HIMALA project will improve the ability of ICIMOD and its partners to understand the impact of weather and climate on floods, droughts, and other water- and climate-induced natural hazards in the Himalayan region in Afghanistan, Bangladesh, Bhutan, China, India, Myanmar, Nepal, and Pakistan.

Brown, Molly Elizabeth↗

Clear Sky Tampa Bay: Project and Toolkit Overview - Resilience-Based Resources for Assessing Solar + Storage on Critical Facilities

The Clear Sky Tampa Bay Team, convened by the Tampa Bay Regional Planning Council (TBRPC) and composed of county and city partners, private and public sector stakeholders, solar energy experts, and academic partners, collaboratively developed a set of resources to support the expanded use of solar + storage in the context of community resilience in Florida. Key questions guiding the project included: (1) What types of buildings and systems provide the maximum resilience benefits? (2) Where within our communities should we prioritize future solar + storage analysis or investment to mitigate the impacts of disasters? (3) How might other societal variables be incorporated into a site assessment when considering solar + storage for resilience? (4) Can a standardized process be developed that leverages existing resources to assist local governments with solar + storage for resilience research and decision-making?

14 SOLAR ENERGY↗

Dynamic and Responsive Distributed Energy Resource Education Solutions for Building, Fire, and Safety Department Officials (Final Technical Report)

From April 2021 through March 2024, the Interstate Renewable Energy Council (IREC) led a collaborative project to develop a free online clearinghouse of educational resources about solar photovoltaics (PV), energy storage systems (ESS), electric vehicle supply equipment (EVSE), and grid-interactive efficient building (GEB) technologies. Two websites—the Clean Energy Clearinghouse and CleanEnergyTraining.org—housed over 70 educational resources. Over the course of the three-year project, 154,272 unique visitors accessed the learning materials. Learner feedback was overwhelmingly positive. Even through the end of the project, there was sustained demand for education and communication. A primary innovation of the project was to drive multiple complementary audiences to the same place. Building owners, designers, installation contractors and developers, authorities having jurisdiction (AHJs), and fire service personnel all benefit from a shared understanding of clean energy technologies, including safety and code-related requirements. When considering the impact on the target audience, the project team worked with partners and advisors to inform resource creation and delivery in such a way as to address key motivational factors of the target audience and compel each user to seek additional information on the topic and return to the Clean Energy Clearinghouse website as their central location for more information. Resources were intentionally developed to be concise—five to 15 minutes—and accessible, meaning not overly technical. Providing basic information demystified the technologies and invited the professional to explore additional learning opportunities. Awardee and partner collaboration was key to project success. IREC facilitated collaboration among the other Topic 2 awardees, Southface and New Buildings Institute (NBI). The three awardees shared relevant information gained through discovery and validation questionnaires that informed product development and reduced duplication of effort by coordinating the development of complementary, and not competing, educational resources. Inspired by this collaboration, IREC brought on additional partners even in the final year of the project. Five regional energy efficiency organizations were part of the project, which expanded the connection between efficiency and distributed energy resources. We also included resources on the Clearinghouse that were developed through other federally funded projects, such as the Buildings Energy Efficiency Frontiers & Innovation Technologies (BENEFIT) program. The website was developed with the learner in mind, and not solely the funding source. Feedback from stakeholders throughout the project, and especially in its final year, indicated the need for continued education and facilitated communication among stakeholders to further the safe and widespread adoption of clean energy.

14 SOLAR ENERGY↗

AlphaFold and Structural Mass Spectrometry Enable Interrogations on the Intrinsically Disordered Regions in Cyanobacterial Light-harvesting Complex Phycobilisome

Intrinsically disordered proteins/regions (IDPRs) are a very large and functionally important class of proteins that participate in weak multivalent interactions in protein complexes. They are recalcitrant for interrogations using X-ray crystallography and cryo-EM. The IDPRs observed at the interface of the photosynthetic pigment protein complexes (PPCs) remain much less clear, e.g., the major cyanobacterial light-harvesting complex (PBS) contains an unstructured PB-loop insertion in the phycocyanobilin domain (PB domain) of ApcE (the largest polypeptide in PBS). Here, a joint platform is built to probe such structural domains. This platform is characterized by two-round progressive justifications of in silico models by using the structural mass spectrometry data. First, the AlphaFold-generated 3D structure of the PB domain (containing PB-loop) was justified in the context of PBS. Second, docking the AlphaFold-generated ApcG (a ligand) into the first-step justified structure (a receptor). The final ligand-receptor complex was then subjected to a second-round justification, again, by using unequivocal isotopically-encoded cross-links identified in LC-MS/MS. This work reveals a full-length PB-loop structure modelled in the PBS basal cylinder, free from any spatial conflicts against the other subunits in PBS. The structure of PB domain highlights the close associations of the intrinsically disordered PB-loop with its binding partners in PBS, including ApcG, another IDPR. The PB-loop region involved in the binding of photosystem II (PSII) is also discussed in the context of excitation energy transfer regulation. Finally, this work calls attention to the highly disordered, yet interrogatable interface between the light-harvesting antenna complexes and the reaction centers.

59 BASIC BIOLOGICAL SCIENCES↗