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At least 1,081 records · Page 60

SU-F-T-462: Lessons Learned From a Machine Incident Reporting System

Purpose: Linear accelerators must operate with minimal downtime. Machine incident logs are a crucial tool to meet this requirement. They providing a history of service and demonstrate whether a fix is working. This study investigates the information content of a large department linear accelerator incident log. Methods: Our department uses an electronic reporting system to provide immediate information to both key department staff and the field service department. This study examines reports for five linac logs during 2015. The report attributes for analysis include frequency, level of documentation, who solved the problem, and type of fix used. Results: Of the reports, 36% were documented as resolved. In another 25% the resolution allowed treatment to proceed although the reported problem recurred within days. In 5% only intermediate troubleshooting was documented. The remainder lacked documentation. In 60% of the reports, radiation therapists resolved the problem, often by clearing the appropriate faults or reinitializing a software or hardware service. 22% were resolved by physics and 10% by field service engineers. The remaining 8% were resolved by IT, Facilities, or resolved spontaneously. Typical fixes, in order of scope, included clearing the fault and moving on, closing and re-opening the patient session or software, cycling power to a sub-unit, recalibrating a device (e.g., optical surface imaging), and calling in Field Service (usually resolving the problem through maintenance or component replacement). Conclusion: The reports with undocumented resolution represent a missed opportunity for learning. Frequency of who resolves a problem scales with the proximity of the person’s role (therapist, physicist, or service engineer), which is inversely related to the permanence of the resolution. Review of lessons learned from machine incident logs can form the basis for guidance to radiation therapists and medical physicists to minimize equipment downtime and ensure safe operation.

61 RADIATION PROTECTION AND DOSIMETRY↗

Extensive lava flow fields on Venus: Preliminary investigation of source elevation and regional slope variations

Large-volume lava flow fields have been identified on Venus, the most areally extensive of which are known as fluctus and have been subdivided into six morphologic types. Sheetlike flow fields (Type 1) lack the numerous, closely spaced, discrete lava flow lobes that characterize digitate flow fields. Transitional flow fields (Type 2) are similar to sheetlike flow fields but contain one or more broad flow lobes. Digitate flow fields are divided further into divergent (Types 3-5) and subparallel (Type 6) classes on the basis of variations in the amount of downstream flow divergence. As a result of our previous analysis of the detailed morphology, stratigraphy, and tectonic associations of Mylitta Fluctus, we have formulated a number of questions to apply to all large flow fields on Venus. In particular, we would like to address the following: (1) eruption conditions and style of flow emplacement (effusion rate, eruption duration), (2) the nature of magma storage zones (presence of neutral buoyancy zones, deep or shallow crustal magma chambers), (3) the origin of melt and possible link to mantle plumes, and (4) the importance of large flow fields in plains evolution. To answer these questions we have begun to examine variations in flow field dimension and morphology; the distribution of large flow fields in terms of elevation above the mean planetary radius; links to regional tectonic or volcanic structures (e.g., associations with large shield edifices, coronae, or rift zones); statigraphic relationships between large flow fields, volcanic plains, shields, and coronae; and various models of flow emplacement in order to estimate eruption parameters. In this particular study, we have examined the proximal elevations and topographic slopes of 16 of the most distinctive flow fields that represent each of the 6 morphologic types.

Magee-Roberts, K.↗

Impact of Bias in the Marine Air Temperature Observation Set on Atmospheric Reanalyses

Marine air temperature observations are collected by volunteer observing ships carrying automatic meteorological sensor packages. Close proximity of the package mount to a dark colored ship's deck is known to introduce a warm bias on sunny afternoons in summer under low wind conditions. This observation set is used as an influential constraint in the MERRA-2 reanalysis, as well as several other reanalyses, in the poorly sampled surface marine boundary layer. Here we explore the consequences of bias in the marine air temperature observation set during the 36 year period 1980-2015. Marine air temperature observations have mainly been collected along major shipping routes, with the densest coverage in the North Atlantic and North Pacific. In the first part of this presentation we review the historical observation coverage and compare air temperature and contemporaneous SST measurements to identify systematic differences. The comparison suggests the presence of diurnal bias in the air temperature observations peaking in subtropics and midlatitudes latitudes in mid-afternoon local time. In the second part we explore the impact of the marine air temperature observations on MERRA-2 through analysis of the terms in the temperature forecast tendency equation, the equation which describes the contributions to changes in temperature during each analysis cycle. Examination of the temperature forecast tendency equation allows us to separate systematic errors due to such processes and radiation, mixing, and advection and allows us to identify the major source of systematic forecast tendencies. The results show that the marine air temperature measurements introduce an error that can reach 0.4K/dy which also peaks in local afternoon at subtropical latitudes. This bias varies with season and has gradually reduced from the beginning of the MERRA-2 reanalysis in 1980. In the final part we conduct numerical experiments with a version of the MERRA-2 analysis system, comparing experiments when the marine air temperature observations are included or withheld from the analysis system.

Akella, Santharam↗

Enriched East Asian oxygen isotope of precipitation indicates reduced summer seasonality in regional climate and westerlies

Speleothem oxygen isotope records over East Asia reveal apparently large and rapid paleoclimate changes over the last several hundred thousand years. However, what the isotopic variation actually represent in terms of the regional climate and circulation is debated. We present an answer that emerges from an analysis of the interannual variation in amount-weighted annual δ 18 O of precipitation over East Asia as simulated by an isotope-enabled model constrained by large-scale atmospheric reanalysis fields. 18 O-enriched years have reduced summer seasonality both in terms of precipitation isotopes and in the large-scale circulation. Changes occur between June and October, where the δ 18 O of precipitation (δ 18 O p ) transitions from the isotopically heavier winter to the lighter summer regime. For 18 O-enriched years, this transition is less pronounced. Variations in precipitation amount alone are insufficient to explain the amount-weighted annual δ 18 O p between 18 O-enriched and 18 O-depleted years. Reduced summer seasonality is also expressed in the low-level monsoonal southerlies and upper-level westerlies; for the latter, the northward migration across the Tibetan Plateau in the summer is less pronounced. Our result thus implicates the westerlies across the plateau as the proximate cause of East Asian paleomonsoon changes, manifested as a modulation of its summer peak.

54 ENVIRONMENTAL SCIENCES↗

A New Treatment Paradigm: Neoadjuvant Radiosurgery Before Surgical Resection of Brain Metastases With Analysis of Local Tumor Recurrence

Purpose: Resected brain metastases (BM) require radiation therapy to reduce local recurrence. Whole brain radiation therapy (WBRT) reduces recurrence, but with potential toxicity. Postoperative stereotactic radiosurgery (SRS) is a strategy without prospective data and problematic target delineation. SRS delivered in the preoperative setting (neoadjuvant, or NaSRS) allows clear target definition and reduction of intraoperative dissemination of tumor cells. Methods and Materials: Our treatment of resectable BM with NaSRS was begun in 2005. Subsequently, a prospective trial of NaSRS was undertaken. A total of 47 consecutively treated patients (23 database and 24 prospective trial) with a total of 51 lesions were reviewed. No statistical difference was observed between the 2 cohorts, and they were combined for analysis. The median follow-up time was 12 months (range, 1-58 months), and the median age was 57. A median of 1 day elapsed between NaSRS and resection. The median diameter of lesions was 3.04 cm (range, 1.34-5.21 cm), and the median volume was 8.49 cc (range, 0.89-46.7 cc). A dose reduction strategy was used, with a median dose of 14 Gy (range, 11.6-18 Gy) prescribed to 80% isodose. Results: Kaplan-Meier overall survival was 77.8% and 60.0% at 6 and 12 months. Kaplan-Meier local control was 97.8%, 85.6%, and 71.8% at 6, 12, and 24 months, respectively. Five of 8 failures were proved pathologically without radiation necrosis. There were no perioperative adverse events. Ultimately, 14.8% of the patients were treated with WBRT. Local failure was more likely with lesions >10 cc (P=.01), >3.4 cm (P=.014), with a trend in surface lesions (P=.066) and eloquent areas (P=.052). Six of the 8 failures had an obvious dural attachment or proximity to draining veins. Conclusions: NaSRS can be performed safely and effectively with excellent results without documented radiation necrosis. Local control was excellent even in the setting of large (>3 cm) lesions. The strong majority of patients were able to avoid WBRT. NaSRS merits consideration in a multi-institution trial.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Measurement of the depth of maximum of air-shower profiles with energies between 10 18.5 and 10 20 eV using the surface detector of the Pierre Auger Observatory and deep learning

We report an investigation of the mass composition of cosmic rays with energies from 3 to 100 EeV ( 1 EeV = 10 18 eV ) using the distributions of the depth of shower maximum X max . The analysis relies on ∼ 50 , 000 events recorded by the surface detector of the Pierre Auger Observatory and a deep-learning-based reconstruction algorithm. Above energies of 5 EeV, the dataset offers a 10-fold increase in statistics with respect to fluorescence measurements at the Observatory. After cross-calibration using the fluorescence detector, this enables the first measurement of the evolution of the mean and the standard deviation of the X max distributions up to 100 EeV. Our findings are threefold: (i) The evolution of the mean logarithmic mass toward a heavier composition with increasing energy can be confirmed and is extended to 100 EeV. (ii) The evolution of the fluctuations of X max toward a heavier and purer composition with increasing energy can be confirmed with high statistics. We report a rather heavy composition and small fluctuations in X max at the highest energies. (iii) We find indications for a characteristic structure beyond a constant change in the mean logarithmic mass, featuring three breaks that are observed in proximity to the ankle, instep, and suppression features in the energy spectrum. Published by the American Physical Society 2025

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Solar Tower Experiments for Radiometric Calibration and Validation of Infrared Imaging Assets and Analysis Tools for Entry Aero-Heating Measurements

The NASA Engineering and Safety Center sponsored Hypersonic Thermodynamic Infrared Measurements assessment team has a task to perform radiometric calibration and validation of land-based and airborne infrared imaging assets and tools for remote thermographic imaging. The IR assets and tools will be used for thermographic imaging of the Space Shuttle Orbiter during entry aero-heating to provide flight boundary layer transition thermography data that could be utilized for calibration and validation of empirical and theoretical aero-heating tools. A series of tests at the Sandia National Laboratories National Solar Thermal Test Facility were designed for this task where reflected solar radiation from a field of heliostats was used to heat a 4 foot by 4 foot test panel consisting of LI 900 ceramic tiles located on top of the 200 foot tall Solar Tower. The test panel provided an Orbiter-like entry temperature for the purposes of radiometric calibration and validation. The Solar Tower provided an ideal test bed for this series of radiometric calibration and validation tests because it had the potential to rapidly heat the large test panel to spatially uniform and non-uniform elevated temperatures. Also, the unsheltered-open-air environment of the Solar Tower was conducive to obtaining unobstructed radiometric data by land-based and airborne IR imaging assets. Various thermocouples installed on the test panel and an infrared imager located in close proximity to the test panel were used to obtain surface temperature measurements for evaluation and calibration of the radiometric data from the infrared imaging assets. The overall test environment, test article, test approach, and typical test results are discussed.

Splinter, Scott C.↗

Interfacial Analysis of Blister Formation in U-10Mo Mini-Plates

A thorough investigation of potential failure mechanisms builds confidence in the performance of monolithic U-10wt%Mo (U-10Mo) fuel plates. The lowenriched uranium (LEU) fuel system is currently undergoing qualification as a high U-density fuel that can be used to convert United States high-performance research reactors from high-enriched uranium (HEU) operation. This will require establishing fuel operational limits such that fission products and a coolable geometry are retained, even during off-normal reactor operation conditions [1]. Such off-normal conditions can subject the fuel plates to temperatures where the internal pressure of precipitated fission gasses result in a permanent deformed, raised area of the cladding, referred to as a blister. These blisters can reduce the local coolability of a fuel plate and can even close coolant channels of a fuel assembly, so blistered plates are considered failed regardless of whether the fuel is truly breached. Historically, a marginto- failure is established through out-of-pile blister threshold testing of irradiated fuel plates. This is accomplished by incrementally heating irradiated fuel plates until blisters are observed. This reveals the temperature threshold that would have resulted in a blister if a plate experienced them at those irradiation conditions [2]. While the blister testing itself reveals the temperatures at which the cladding mechanical integrity was exceeded, a more thorough investigation of the interface evolution in proximity to formed blisters may reveal mechanisms as to the blister formation and retention of fission gases. Previous studies have explored potential underlying mechanisms in historical plates that may have been close to blistering [3]; however, this work is the first exploration of blister tested irradiated plates, fabricated by a commercial vendor—another requirement for qualification of the fuel system [1]. The Mini-plate 1 (MP-1) experiment was the first in a series of irradiation and post-irradiation examination (PIE) campaigns to qualify the U-10Mo monolithic fuel system. It consisted of commercially fabricated 25.4×101.5 mm Al-clad mini-plates with a monolithic U-10Mo foil coated in a Zr diffusion barrier. The primary MP-1 PIE campaign was previously completed. Among the suit of examinations was a blister testing campaign, where plates were incrementally annealed in 25°C increments until blisters were observed or a maximum temperature of 550°C was reached [4]. The previously blistered plates from this campaign were revisited in this work.

25228↗

Electric Power Generation from Low to Intermediate Temperature Resources

This project was designed to test the concept on the Eland-Lodgepole Field near Dickinson, North Dakota in the Williston Basin. The field is in secondary-recovery water-flood and consists of 12 producing oil wells, 5 water injection wells and one disposal well. Water production at the site averages approximately 320 gallons per minute (20.2 l s-1) and the temperature is 100 ⁰C. Engineers at Ormat estimated power production potential with the existing resource to be approximately 350 kWh. Unfortunately, ownership of the field was transferred from Encore, Inc., to Denbury, Inc., within the first week of the project. After two years of discussion and planning, Denbury decided not to pursue this project due to complications with the site location and its proximity to Patterson Lake. Attempts to find other partners operating in the Williston Basin were unsuccessful. Consequently, we were unable to pursue the primary objective of the project. However, during negations with Denbury and subsequent time spent contacting other potential partners, we focused on objectives 2 and 3 and developed a clear understanding of the potential for co-produced production in the Williston Basin and the best practices for developing similar projects. At least nine water bearing formations with temperatures greater than 90 ⁰C extend over areas of several 10s of km2. The total energy contained in the rock volume of those geothermal aquifers is 283.6 EJ (1 EJ = 1018 J). The total energy contained in the water volume, determined from porosities which range from 2 percent to 8 percent, is 6.8 EJ. The aquifers grouped by 10 ⁰C temperature bins (Table 1) include one or more formations due to the bowl-shape structure of the basin. Table 1. Summary of energy available in geothermal aquifers in the Williston Basin Analysis of overall fluid production from active wells, units, fields and formations in North Dakota showed that few sites co-produce sufficient fluid for significant power production with ORC technology. Average co-produced water for 10,480 wells is 3.2 gallons per minute (gpm). Even excluding the tight formations, Bakken and Three Forks, average co-produced water for the remaining 3,337 is only 5 gpm. The output of the highest producing well is 184 gpm and the average of the top 100 wells is 52 gpm. Due to the depth of the oil producing formations in the Williston Basin, typically 3 km or greater, pumps are operated slowly to prevent watering out thus total fluid production is purposefully maintained at low volumes. There remain potential possibilities for development of geothermal fluids in the Williston Basin. Unitized fields in which water production from several tens of wells is collected at a single site are good possibilities for development. Water production in the unitized fields is greater than 1000 gpm is several areas. A similar possibility occurs where infill-drilling between Bakken and Three Forks horizontal wells has created areas where large volumes of geothermal fluids are available on multi-well pads and in unitized fields. Although the Bakken produces small amounts of water, the water/oil ration is typically less than 1, the oil and water mix produced at the well head can be sent through the heat exchanger on an ORC. It is estimated that several tens of MWh of power could be generated by a distributed system of ORC engines in the areas of high-density drilling in the Bakken Formation. Finally, horizontal drilling in water bearing formations is the other possibility. Several secondary recovery water-flood projects in the basin are producing water above 100 ⁰C at rates of 300 gpm to 850 gpm. Those systems also could produce several tens of MWh of power with ORC technology. Objective 3 of the project was highly successful. The program has produced 5 PhDs, 7 MS, and 3 BS students with theses in geothermal energy. The team has involved 7 faculty in 4 different engineering and science disciplines, ChE, EE, GE, and Geol. The team has produced 26 peer-reviewed papers and 62 presentations at professional meetings. Faculty involved in the program developed five graduate level courses covering different elements in heat flow and geothermal energy that are now offered in the Harold Hamm School of Geology and Geological Engineering. Lessons learned – Keys to developing a successful project;1. Determine target formations; a. Data from oil and gas operators, state oil and gas regulatory agencies, and state geological surveys help to identify producing formations and their properties; 2. Determine the quantity of energy available in the target formations; a. A complete thermal analysis of the basin or region yields the most useful information; b. Critical data include: BHT, heat flow, stratigraphy, lithology, lithological properties, and thermal conductivity, subsurface structure; 3. Determine fluid production potential; a. State oil and gas regulatory agencies, and state geological surveys have data on oil, gas and water production. State Water Commission/Agencies have data on water quality, aquifers, and regulations; b. Consider single horizontal wells, multiple conventional wells, and unitized fields; 4. Calculate energy production capacity of each formation based on different well combination and power plant scenarios. This is a broad overview rather than a site specific analysis; 5. Research and understand the local electrical power industry. Obtain the PPA before committing to the project; 6. Work with the high-level personnel in the oil company partner. Obtain an MOU that addresses all issues in the project including what to expect if the company goes out of business, is bought out, changes management, etc; and 7. Be prepared for project delays.

15 GEOTHERMAL ENERGY↗

A haplotype-resolved reference genome for Eucalyptus grandis

Eucalyptus grandis is a hardwood tree used worldwide as pure species or hybrid partner to breed fast-growing plantation forestry crops that serve as feedstocks of timber and lignocellulosic biomass for pulp, paper, biomaterials, and biorefinery products. The current v2.0 genome reference for the species served as the first reference for the genus and has helped drive the development of molecular breeding tools for eucalypts. Using PacBio HiFi long reads and Omni-C proximity ligation sequencing, we produced an improved, haplotype-phased assembly (v4.0) for TAG0014, an early-generation selection of E. grandis. The 2 haplotypes are 571 Mbp (HAP1) and 552 Mbp (HAP2) in size and consist of 37 and 46 contigs scaffolded onto 11 chromosomes (contig N50 of 28.9 and 16.7 Mbp), respectively. These haplotype assemblies are 70-90 Mbp smaller than the diploid v2.0 assembly but capture all except one of the 22 telomeres, suggesting that substantial redundant sequence was included in the previous assembly. A total of 35,929 (HAP1) and 35,583 (HAP2) gene models were annotated, of which 438 and 472 contain long introns (>10 kbp) in gene models previously (v2.0) identified as multiple smaller genes. These and other improvements have increased gene annotation completeness levels from 93.8 to 99.4% in the v4.0 assembly. We found that 6,493 and 6,346 genes are within tandem duplicate arrays (HAP1 and HAP2, respectively, 18.4 and 17.8% of the total) and >43.8% of the haplotype assemblies consists of repeat elements. Analysis of synteny between the haplotypes and the E. grandis v2.0 reference genome revealed extensive regions of collinearity, but also some major rearrangements, and provided a preview of population and pangenome variation in the species.

Lötter, Anneri↗

Correlation between Sensor Performance, Autonomy Performance and Fuel-Efficiency in Semi-Truck Platoons

Semi-trucks, specifically class-8 trucks, have recently become a platform of interest for autonomy systems. Platooning involves multiple trucks following each n close proximity, with only the lead truck being manually driven and the rest being controlled autonomously. This approach to semi-truck autonomy is easily integrated on existing platforms, reduces delivery times, and reduces green- house gas emissions via fuel economy benefits. Level 1 SAE fuel studies were performed on class-8 trucks operating with the Auburn Cooperative Adaptive Cruise Control (CACC) system, and fuel savings up to 10-12% were seen. Enabling platooning autonomy required the use of radar, global positioning systems (GPS), and wireless vehicle-to-vehicle (V2V) communication. Poor measurements and state estimates can lead to incorrect or missing positioning data, which can lead to unnecessary dynamics and finally wasted fuel. This is especially an issue if deceleration is applied in response to a bad measurement. In this study, a faulty radar was shown to cause a greater than 5% increase in fuel consumption. The mechanism of this fuel consumption increase is investigated and applied to other types of sensor failures to indicate their potential effects on fuel economy. This analysis indicates that poor GPS signals over short time can be largely filtered out, with no real gain or loss of fuel economy. V2V communications were intentionally limited by causing interference, which resulted in dropped communication packets over a small physical area, but not an appreciable impact on fuel economy.

vehicle platooning, sensor performance, semi-auton↗

Multimodality Local Therapy for Retroperitoneal Sarcoma

Purpose: Soft-tissue sarcomas of the retroperitoneum are rare tumors comprising less than 1% of all malignancies. Although surgery continues as the mainstay of treatment, the large size of these tumors coupled with their proximity to critical structures make resection with wide margins difficult to achieve. The role and timing of radiotherapy are controversial. This study updates our institutional experience using multimodality local therapy for resectable retroperitoneal sarcoma and identifies prognostic factors impacting disease control and survival. Methods and Materials: Between 1974 and 2007, 58 patients with nonmetastatic retroperitoneal sarcoma were treated with surgery and radiation at University of Florida. The median age at radiotherapy was 57 years old (range, 18-80 years). Forty-two patients received preoperative radiotherapy and 16 received postoperative radiotherapy. Nineteen patients received 1.8 Gy once daily and 39 patients received 1.2 Gy twice daily. Variables analyzed for prognostic value included age, grade, kidney involvement, histology, de novo versus recurrent presentation, tumor diameter, margin status, radiotherapy sequencing (preoperative vs. postoperative), total radiation dose, fractionation scheme, and treatment era. Results: The 5-year overall survival, cause-specific survival, and local control rates were 49%, 58%, and 62%, respectively. Nearly two-thirds of disease failures involved a component of local progression. On multivariate analysis, only margin status was significantly associated with improved 5-year local control (85%, negative margins; 63%, microscopic positive margins; 0%, gross positive margins; p < 0.0001) and 5-year overall survival (64%, negative margins; 56%, microscopic positive margins; 13%, gross positive margins; p = 0.0012). Thirty-one Grade 3 or greater toxicities were observed in 22 patients, including two treatment-related deaths (3%). Conclusion: For retroperitoneal sarcoma, local control remains a challenge and combined-modality therapy may be associated with significant acute and late morbidity. Our patterns of failure data suggest that improvements in local control may translate into a survival benefit.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

The LSBmax algorithm for boosting resilience of electric grids post (N‐2) contingencies

Abstract A computationally improved algorithm is presented to find the best transmission switching (TS) candidate for boosting resilience of electricity grids subject to ( N ‐2) contingencies. Here, resilience is computed as the reduction in load shed after the above‐mentioned ( N‐ ) contingencies. TS is a planned line outage, and past research shows that changing the transmission system's topology changes the power flow and removes post contingency violations. Finding the best TS candidate in a computationally suitable time for effectively boosting resilience is a challenge. The best TS candidate is found using a novel heuristic method by decreasing the search space based on proximity to the bus with the maximum load shedding (LSB). The LSB algorithm is faster than existing algorithms in the literature; and, it is compatible with both the AC and DC optimal power flow formulations. To validate the authors' claims of speedup and accuracy, two metrics are used to analyze the results from the IEEE 39‐bus and 118‐bus systems. Finally, the inherent parallelism of the LSB algorithm is leveraged on a high‐performance computing platform and applied to the large‐scale Polish 2383‐bus test system to validate scalability in both size and speedup in computation time.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Critical Velocities in Open Capillary Flow

This paper describes the proposed research program on open capillary flow and the preliminary work performed theoretically and in drop tower experiments. The work focuses on the fundamental physical understanding of the flow through capillary bound geometries, where the circumference of the cross section of the flow path contains free surfaces. Examples for such a flow configuration are capillary vanes in surface tension tanks, flow along edges and corners and flow through liquid bridges. The geometries may be classified by their cross section areas, wetted circumferences and the radii of curvature of the free surfaces. In the streaming float zone the flow path is bound by a free surface only. The ribbon vane is a model for vane types used in surface tension tanks, where a structure in proximity to the tank wall forms a capillary gap. A groove is used in heat pipes for the transportation of the condensed working fluid to the heat source and a wedge may occur in a spaceborne experiment where fluid has to be transported by the means of surface tension. The research objectives are the determination of the maximum volume flux, the observation of the free surfaces and the liquid flow inside the flow path as well as the evaluation of the limiting capillary wave speed. The restriction of the maximum volume flux is due to convective forces (flow velocity exceeding the capillary wave speed) and/or viscous forces, i.e. the viscous head loss along the flow path must be compensated by the capillary pressure due to the curved free surface. Exceeding the maximum volume flux leads to the choking of the flow path, thus the free surface collapses and.gas ingestion occurs at the outlet. The means are ground-based experimental work with plateau tanks and in a drop tower, a sounding rocket flight, and theoretical analysis with integral balances as well as full three dimensional CFD solutions for flow with free surfaces.

Dreyer, Michael↗

Pilot Study: Unique Response of Bone Tissue During an Investigation of Radio-Adaptive Effects in Mice

PURPOSE: We obtained bone tissue to evaluate the collateral effects of experiments designed to investigate molecular mechanisms of radio-adaptation in a mouse model. Radio-adaptation describes a process by which the prior exposure to low dose radiation can protect against the toxic effect of a subsequent high dose exposure. In the radio-adaptation experiments, C57Bl/6 mice were exposed to either a Sham or a priming Low Dose (5 cGy) of Cs-137 gamma rays before being exposed to either a Sham or High Dose (6 Gy) 24 hours later. ANALYSIS: Bone tissue were obtained from two experiments where mice were sacrificed at 3 days (n=3/group, 12 total) and at 14 days (n=6/group, 24 total) following high dose exposure. Tissues were analyzed to 1) evaluate a radio-adaptive response in bone tissue and 2) describe cellular and microstructural effects for two skeletal sites with different rates of bone turnover. One tibia and one lumbar vertebrae (LV2), collected at the 3-day time-point, were analyzed by bone histomorphometry and micro-CT to evaluate the cellular response and any evidence of microarchitectural impact. Likewise, tibia and LV2, collected at the 14-day time-point, were analyzed by micro-CT alone to evaluate resulting changes to bone structure and microarchitecture. The data were analyzed by 2-way ANOVA to evaluate the effects of the priming low dose radiation, of the high dose radiation, and of any interaction between the priming low and high doses of radiation. Bone histomorphometry was performed in the cancellous bone (aka trabecular bone) compartments of the proximal tibial metaphysis and of LV2. RESULTS: Cellular Response @ 3 Days The priming Low Dose radiation decreased osteoblast-covered bone perimeter in the proximal tibia and the total cell density in the bone marrow in the LV2. High Dose radiation, regardless of prior exposure to priming dose, dramatically reduced total cell density in bone marrow of both the long bone and vertebra. However, in the proximal tibia, High Dose radiation increased the osteoclast-covered bone perimeters, the density of adipocytes in bone marrow, and the area of bone marrow occupied by fat cells -- while in the LV2, adipocytes were rare and not stimulated by High Dose radiation. In an unexpected response, High Dose radiation dramatically increased (10-fold) osteoblast-covered bone perimeter in the LV2.

Sibonga, J. D.↗

Impacts of electromagnetic fields associated with marine and hydrokinetic surrogate technologies on fish movements and behaviors.

Marine and hydrokinetic energy (MHK) and offshore wind devices are being developed and deployed in U.S. and international waters. Electric current flowing through subsea transmission cables associated with these devices will generate electromagnetic fields (EMF), which may interact with, and potentially impact, marine fishes. Some marine fishes can detect electric and/or magnetic fields and use them to navigate, orientate, and sense prey, mates and predators. Over the past five years there have been multiple comprehensive reviews and studies evaluating the potential vulnerability of marine fishes to EMF produced by MHK devices. Most documented effects involve sub-lethal behavioral responses of individual fish when in close proximity to EMF (e.g., fish being repelled by or attracted to fields). These reviews reach conclusions that the current state of research on this topic is still in its infancy and evaluations of potential impacts are associated with great uncertainty. A variety of MHK technologies are likely to be considered for deployment offshore of the Hawaiian Islands, and there is a need to be able to better predict and assess potential associated environmental impacts. The goal of this study was to provide a complementary piece to these previous reviews (e.g., Normandeau et al. 2011) by focusing on marine fish species in the Hawaii region. We compiled the relevant available information, then prioritized fish species as candidates for various paths of future research. To address this, we first developed a list of Hawaii Region Focal Species, which included fishes that are more likely to be sensitive to EMF. We then compiled species-specific information available in the literature on their sensitivity to EMF, as well as life history, movement and habitat use information that could inform an analysis of their likelihood of encountering EMF from subsea cables associated with MHK devices. Studies have only documented EMF sensitivity in 11 of the marine fish species in this region. There was also relatively little detailed information on fish movement and habitat use patterns for most of the focal species. Our last objective was to develop recommendations for research needs to close the important knowledge gaps. We describe species-independent baseline research that primarily consists of in situ quantification of EMF generated by MHK devices and undersea cables that can occur as pilot and commercial scale MHK devices are deployed in Hawaii. Then we propose a simple approach for prioritizing Hawaii Region Focal Species (ranked relative to each other) as candidates in multiple related research paths. The prioritization approach incorporates EMF sensitivity information with the likelihood of interacting with EMF generated undersea transmission cables associated with MHK devices. Finally, we discuss the types of research needed to help fill gaps in the scientific knowledge base for this region. These involve studies to better define species-specific EMF sensitivity thresholds under various environmental conditions, studies of life history, movement and habitat use patterns to improve our understanding of the likelihood and frequency fishes may be in the vicinity of EMF generated by subsea transmission cables, and studies of the potential for related population, community or ecosystem impacts. Many of these studies can and should occur opportunistically as pilot and commercial scale MHK devices are deployed in Hawaii.

59 BASIC BIOLOGICAL SCIENCES↗

Integrated ‘omics analysis for studying the microbial community response to a pH perturbation of a cellulose-degrading bioreactor culture

Propionate accumulation is a common indicator of process imbalances in anaerobic bioreactor systems. The accumulation of propionate can occur due to low retention rates, hydrogen accumulation, or mechanical changes affecting the proximity between propionate oxidizers and partner species, thereby preventing necessary electron transfer. Few studies, however, have observed the changes in microbial community structure during propionate accumulation. We used 454 pyrosequencing of 16S rDNA to evaluate the community membership during propionate accumulations in replicate bioreactors with rumen based cultures. Half of the culture volume from a parent reactor was transferred to a sterile “daughter” reactor, and both systems were run identically. Both reactors experienced a propionate accumulation after roughly 10 days, with the propionate accumulation being less pronounced in the parent reactor as compared to the daughter reactor. Non-metric multidimensional scaling (NMDS) was used to determine clustering patterns of the samples, and correlative methods were used to determine which OTUs were significantly associated with the movements of samples along the NMDS axes. The presence of Saccharofermentans characterized the position of early samples, whereas the presence of Ruminococcus and Succiniclasticum were more indicative of the positions of later samples. Hydrogen accumulation and low sequence counts indicated low methanogen activity. Although both reactor systems were closed to microbial inputs due to the sterilization of influent media, we recorded significant increases in reactor diversity over time. This suggests that changes in the abundances of dominant community members may affect the sequencing of rare taxa within samples.

59 BASIC BIOLOGICAL SCIENCES↗

Observed Variability in Convective Cell Characteristics and Near-Storm Environments across the Sea- and Bay-Breeze Fronts in Southeast Texas

Abstract During the DOE Atmospheric Radiation Measurement (ARM) Tracking Aerosol Convection Interactions Experiment (TRACER) IOP spanning June–September 2022, two fixed ARM sites and a mobile team concurrently sampled the airmass heterogeneity across sea- and bay-breeze fronts around the greater Houston metropolitan region. Here, we quantify the spatiotemporal variability between maritime (coastal/bay side of breeze fronts) and continental (inland side of breeze fronts) air masses over 15 IOP days characterized by strong sea-breeze forcing. We analyze environmental profile data from 177 radiosondes and use S- and C-band radar data to track and quantify the variability in attributes of more than 2300 shallow and transitioning cells across different air masses. The composite analysis of environmental profiles indicates that during the early afternoon, the sea-breeze maritime air mass exhibits lower convective available potential energy (CAPE) than the bay-breeze maritime air mass. As the sea breeze advances inland with time, CAPE within the maritime air mass exceeds that of the continental air mass to the north of the breeze fronts. In general, maritime cells have a larger mean composite reflectivity and cell widths than continental cells; however, the response varies between shallow and transitioning cells. Mean composite 20-dB Z echo-top heights, however, are similar across air masses for both shallow and transitioning cells. The continental and maritime inflow air mass for transitioning cells has significantly different mean values for mixed-layer entrainment CAPE, lifted condensation level, level of free condensation, boundary layer depth, and diluted equilibrium level. For shallow cells, only total precipitable water shows a significant difference. Significance Statement The greater Houston metropolitan area is a natural laboratory for understanding the individual impacts of background meteorology and aerosols on convective clouds. Due to its proximity to the Gulf Coast and Galveston Bay, the Houston region experiences a diurnal precipitation cycle in the summer, driven by convection triggered from sea- and bay-breeze fronts. These fronts act as a boundary between air masses with distinct thermodynamic and environmental characteristics. Convergence along these fronts and interactions between storm outflow and the fronts facilitate convection initiation in different mesoscale air masses. This study quantifies the heterogeneity among these air masses while investigating their influence on cloud microphysics. We find that the effect of airmass heterogeneity is more pronounced for the bulk microphysical properties in shallow clouds.

Sharma, Milind↗