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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 1,099 records · Page 61
All sourcesNASA NTRSDOE OSTI
DOE OSTI2017

Insight into “Changing-look” AGN Mrk 1018 from the Fe Kα Line: The Reprocessing Gas Has Yet to Fully Respond to the Fading of the AGN

Mrk 1018 is a “changing-look” active galactic nucleus (AGN) whose optical spectrum transitioned from a Type 1.9 to a Type 1 between 1979 and 1984, and then back to a Type 1.9 in 2015. This latest transition was accompanied by a decrease in X-ray flux. We analyze the Chandra spectra from 2010 and 2016 and NuSTAR spectra from 2016, with a careful treatment of pileup in the Chandra spectrum from 2010, and self-consistently model absorption, reflection, and Fe Kα line emission in the X-ray spectra from 2016. We demonstrate that while the 2–10 keV X-ray flux decreased by an order of magnitude (1.46{sub −0.13}{sup +0.10}×10{sup −11}–1.31{sub −0.04}{sup +0.09}×10{sup −12} erg s{sup −1} cm{sup −2}), the Fe Kα equivalent width (EW) increased from 0.18{sub −0.12}{sup +0.17} to 0.61{sub −0.25}{sup +0.27} keV due to a depressed AGN continuum. We jointly fit the Chandra and NuSTAR spectra from 2016 using the physically motivated MYTorus model, and find that the torus orientation is consistent with a face-on geometry and that lines of sight intersecting the torus are ruled out. While we measure no line-of-sight absorption, we do measure a column density of N{sub H}=5.38{sub −4.0}{sup +14}×10{sup 22} cm{sup −2} for gas out of the line of sight that reprocesses the X-ray emission. We find a high relative normalization between the Compton-scattered emission and transmitted continuum, which is indicative of time lags between the primary X-ray source and reprocessing gas. We predict that the Fe Kα line will respond to the decrease in AGN flux, which would manifest as a decrease in the Fe Kα EW.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2017

Optical Line Emission from z ∼ 6.8 Sources with Deep Constraints on Lyα Visibility

We analyze a sample of z-dropout galaxies in the CANDELS GOODS South and UDS fields that have been targeted by a dedicated spectroscopic campaign aimed at detecting their Lyα line. Deep IRAC observations at 3.6 and 4.5 μm are used to determine the strength of optical emission lines affecting these bands at z ∼ 6.5–6.9 in order to (1) investigate possible physical differences between Lyα emitting and non-emitting sources; (2) constrain the escape fraction of ionizing photons; and (3) provide an estimate of the specific star formation rate at high redshifts. We find evidence of strong [O iii]+Hβ emission in the average (stacked) SEDs of galaxies both with and without Lyα emission. The blue IRAC [3.6]–[4.5] color of the stack with detected Lyα line can be converted into a rest-frame equivalent width EW([O iii]+Hβ) = 1500{sub −440}{sup +530} Å assuming a flat intrinsic stellar continuum. This strong optical line emission enables a first estimate of f{sub esc}≲20% on the escape fraction of ionizing photons from Lyα detected objects. The objects with no Lyα line show less extreme EW([O iii]+Hβ) = 520{sub −150}{sup +170} Å, suggesting different physical conditions of the H ii regions with respect to Lyα-emitting ones, or a larger f{sub esc}. The latter case is consistent with a combined evolution of f{sub esc} and the neutral hydrogen fraction as an explanation of the lack of bright Lyα emission at z > 6. A lower limit on the specific star formation rate, SSFR > 9.1 Gyr{sup −1} for M{sub star}=2×10{sup 9} M{sub ⊙} galaxies at these redshifts can be derived from the spectroscopically confirmed sample.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2017

Nonlinear Color–Metallicity Relations of Globular Clusters. VII. Nonlinear Absorption-line Index versus Metallicity Relations and Bimodal Index Distributions of NGC 5128 Globular Clusters

Spectroscopy on the globular cluster (GC) system of NGC 5128 revealed bimodality in absorption-line index distributions of its old GCs. GC division is a widely observed and studied phenomenon whose interpretation has depicted host galaxy formation and evolution such that it harbors two distinct metallicity groups. Such a conventional view of GC bimodality has mainly been based on photometry. The recent GC photometric data, however, presented an alternative perspective in which the nonlinear metallicity-to-color transformation is responsible for color bimodality of GC systems. Here we apply the same line of analysis to the spectral indices and examine the absorption-line index versus metallicity relations for the NGC 5128 GC system. NGC 5128 GCs display nonlinearity in the metallicity-index planes, most prominently for the Balmer lines and by a non-negligible degree for the metallicity-sensitive magnesium line. We demonstrate that the observed spectroscopic division of NGC 5128 GCs can be caused by the nonlinear nature of the metallicity-to-index conversions and thus one does not need to resort to two separate GC subgroups. Our analysis incorporating this nonlinearity provides a new perspective on the structure of NGC 5128's GC system, and a further piece to the global picture of the formation of GC systems and their host galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2019

Machine-learning Classifiers for Intermediate Redshift Emission-line Galaxies

Classification of intermediate redshift (z = 0.3-0.8) emission line galaxies as star-forming galaxies, composite galaxies, active galactic nuclei (AGNs), or low-ionization nuclear emission regions (LINERs) using optical spectra alone was impossible because the lines used for standard optical diagnostic diagrams: [N ii], Hα, and [S ii] are redshifted out of the observed wavelength range. In this work, we address this problem using four supervised machine-learning classification algorithms: k-nearest neighbors (KNN), support vector classifier (SVC), random forest (RF), and a multilayer perceptron (MLP) neural network. For input features, we use properties that can be measured from optical galaxy spectra out to z < 0.8 - [O iii]/Hβ, [O ii]/Hβ, [O iii] line width, and stellar velocity dispersion - and four colors (u - g, g - r, r - i, and i - z) corrected to z = 0.1. The labels for the low redshift emission line galaxy training set are determined using standard optical diagnostic diagrams. RF has the best area under curve score for classifying all four galaxy types, meaning the highest distinguishing power. Both the AUC scores and accuracies of the other algorithms are ordered as MLP > SVC > KNN. The classification accuracies with all eight features (and the four spectroscopically determined features only) are 93.4% (92.3%) for star-forming galaxies, 69.4% (63.7%) for composite galaxies, 71.8% (67.3%) for AGNs, and 65.7% (60.8%) for LINERs. The stacked spectrum of galaxies of the same type as determined by optical diagnostic diagrams at low redshift and RF at intermediate redshift are broadly consistent. Our publicly available code (https://github.com/zkdtc/MLC_ELGs) and trained models will be instrumental for classifying emission line galaxies in upcoming wide-field spectroscopic surveys.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

The Physical Properties of Low-redshift FeLoBAL Quasars. II. The Rest-frame Optical Emission Line Properties

We report the results of an analysis of the Hβ emission line region of a sample of 30 low-redshift (z < 1) iron low-ionization broad absorption line quasars (FeLoBALQs). Eleven of these objects are newly classified as FeLoBALQs. A matched sample of 132 unabsorbed quasars was analyzed in parallel. The emission lines showed the well-known anticorrelation between the [O III ] and Fe II emission. Using a summary statistic called E1 to quantify this anticorrelation, we found that while the distribution of E1 for the unabsorbed quasars has a single peak, the FeLoBALQs have a bimodal shape in this parameter. Previous studies have shown that the line emission properties of BAL and non-BALQs are consistent; therefore, the difference in the Hβ region emission between FeLoBALQs and unabsorbed quasars is a new result. The two populations of FeLoBALQs are characterized by low and high bolometric luminosities and Eddington ratios. Some previous studies have suggested that BALQs are high accretion rate objects and therefore the discovery of the low accretion rate branch of FeLoBAL quasars was unexpected. We also found that the Hβ FWHM is systematically broader among the FeLoBALQs, implying a higher inclination viewing angle or a dearth of low velocity line emitting gas.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2023

Fantastic Fits with fantasy of Active Galactic Nuclei Spectra: Exploring the Fe II Emission near the Hα Line

In this study, a refined approach for multicomponent fitting of active galactic nuclei (AGNs) spectra is presented utilizing the newly developed Python code Fully Automated pythoN Tool for AGN Spectra analYsis (fantasy). AGN spectra are modeled by simultaneously considering the underlying broken power-law continuum, predefined emission line lists, and an Fe II model, which is here extended to cover the wavelength range 3700–11000 Å. The Fe II model, founded solely on atomic data, effectively describes the extensive emission of the complex iron ion in the vicinity of the Hγ and Hβ lines, as well as near the Hα line, which was previously rarely studied. The proposed spectral fitting approach is tested on a sample of high-quality AGN spectra from the Sloan Digital Sky Survey Data Release 17. The results indicate that when Fe II emission is present near Hβ, it is also detected redward from Hα, potentially contaminating the broad Hα line wings and thus affecting the measurements of its flux and width. The production of Fe II emission is found to be strongly correlated with Eddington luminosity and appears to be controlled by a similar mechanism as the hydrogen Balmer lines. The study highlights the benefits of fitting type 1 AGN spectra with the fantasy code, pointing that it may be used as a robust tool for analyzing a large number of AGN spectra in the coming spectral surveys.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS2014

Simultaneous NuSTAR and XMM-Newton 0.5-80 KeV Spectroscopy of the Narrow-Line Seyfert 1 Galaxy SWIFT J2127.4+5654

We present a broad-band spectral analysis of the joint XMM-Newton and Nuclear Spectroscopic Telescope Array observational campaign of the narrow-line Seyfert 1 SWIFT J2127.4+5654, consisting of 300 kiloseconds performed during three XMM-Newton orbits. We detect a relativistic broadened iron K-alpha line originating from the innermost regions of the accretion disc surrounding the central black hole, from which we infer an intermediate spin of a = 0.58 (sup +0.11) (sub −0.17). The intrinsic spectrum is steep (gamma = 2.08 plus or minus 0.01) as commonly found in narrow-line Seyfert 1 galaxies, while the cutoff energy (E (sub c) = 108 (sup +11) (sub −10) kiloelectronvolts) falls within the range observed in broad-line Seyfert 1 galaxies. We measure a low-frequency lag that increases steadily with energy, while at high frequencies, there is a clear lag following the shape of the broad Fe K emission line. Interestingly, the observed Fe K lag in SWIFT J2127.4+5654 is not as broad as in other sources that have maximally spinning black holes. The lag amplitude suggests a continuum-to-reprocessor distance of about 10-20 radius of gyration. These timing results independently support an intermediate black hole spin and a compact corona.

Nuclear Spectroscopic Telescope Array↗
NASA NTRS2017

Unreported Emission Lines of Rb, Ce, La, Sr, Y, Zr, Pb and Se Detected Using Laser-Induced Breakdown Spectroscopy

Information on emission lines for major and minor elements is readily available from the National Institute of Standards and Technology (NIST) as part of the Atomic Spectra Database. However, tabulated emission lines are scarce for some minor elements and the wavelength ranges presented on the NIST database are limited to those included in existing studies. Previous work concerning minor element calibration curves measured using laser-induced break-down spectroscopy found evidence of Zn emission lines that were not documented on the NIST database. In this study, rock powders were doped with Rb, Ce, La, Sr, Y, Zr, Pb and Se in concentrations ranging from 10 percent to 10 parts per million. The difference between normalized spectra collected on samples containing 10 percent dopant and those containing only 10 parts per million were used to identify all emission lines that can be detected using LIBS (Laser-Induced Breakdown Spectroscopy) in a ChemCam-like configuration at the Mount Holyoke College LIBS facility. These emission spectra provide evidence of many previously undocumented emission lines for the elements measured here.

LIBS↗
NASA NTRSDate not supplied

High Pressure Vent Line Mishap: Investigation Summary

During testing on the E-1 Test Stand, the High Pressure (HP) Liquid Hydrogen (LH) vent line was displaced off its supports, damaging the expansion joints (EJ), piping, and pipe supports of the line. The damage to the vent line is believed to have occurred during Cell 3 testing on Thursday, September 21, during the first test of the day. The fuel systems being used for Cell 3 testing are connected to the LP and HP LH Vent systems and are being used in Liquid Natural Gas (LNG) service. The Cell 3 interface bleed valve that discharges to the HP LH Vent system was inadvertently left at 100% open during the chill-in process for longer than the typical amount of time. Since this valve is usually at a lower set point or intermittently closed during the chill-in process, more than the typical amount of LNG flowed into the vent line as pre-test procedures continued as normal. The open bleed valve was noticed before test preparations were completed and prior to refilling of the HP LH Tank before test. The test sequence was started, run, and shutdown normally. Video of the flare stack showed an atypical discharge of liquid at the flare stack exit. Pressure downstream of the Cell 3 interface bleed valve (a valve connected to the southernmost part of the vent system) spiked to a higher-than-normal level. No evidence of damage was captured at the time of the event. The system was used in a typical fashion after the event and for 4 subsequent tests on Thursday and Friday, before the damage was observed. A drive-by inspection of the flare stack was performed on Friday (September 22nd) morning before sunrise and before test activities began, but no damage of the vent line was observed at that time. No abnormal system behavior was observed during testing on Friday. The damage was discovered and reported to NASA Test Operations on Monday, initiating the Incident Response Team. The Mishap appointment letter was issued September 27th to formally initiate the Mishap Investigation.

LNG↗
NASA NTRSDate not supplied

On the Application of an Actuator Line Model for Rotorcraft Outwash Predictions

As the next generation of Vertical Take-Off and Landing (VTOL) vehicles develops and the VTOL industry makes significant progress in utilizing them for public transportation, it is crucial to have validated and cost-effective computational models accessible to both industry professionals and academics in the rotorcraft community. This study investigates the implementation of a new actuator line model within NASA’s OVERFLOW computational fluid dynamics (CFD) solver, with a focus on predicting rotorcraft outwash. This reduced-order rotor model was developed to provide reasonably accurate outwash predictions while reducing computational costs compared to traditional blade-resolved CFD simulations. To validate the actuator line model for outwash predictions, the model was compared against existing experimental data across three validation cases: single rotor Out-of-Ground Effect (OGE), single rotor In-Ground Effect (IGE), and tandem rotor IGE. All three cases were based on the rotor geometry and configuration of the CH-47D. To evaluate the feasibility and advantages of using an actuator line model for outwash predictions the results were compared to a series of high-fidelity blade-resolved CFD simulations as well as comprehensive analyses simulations based on CHARM predictions. The results of this study demonstrate the feasibility of leveraging this new actuator line model for reasonably accurate outwash predictions. The outwash results obtained from the actuator line model closely matched those from high-fidelity blade-resolved simulations while significantly reducing the overall computational costs of the simulations. Results from this study further demonstrated the feasibility of using CHARM for efficient, and reasonably accurate time-averaged outwash predictions for multi-rotor configurations.

CFD↗
DOE OSTI2014

INVESTIGATION OF A DYNAMIC POWER LINE RATING CONCEPT FOR IMPROVED WIND ENERGY INTEGRATION OVER COMPLEX TERRAIN

Dynamic Line Rating (DLR) is a smart grid technology that allows the rating of power line to be based on real-time conductor temperature dependent on local weather conditions. In current practice overhead power lines are generally given a conservative rating based on worst case weather conditions. Using historical weather data collected over a test bed area, we demonstrate there is often additional transmission capacity not being utilized with the current static rating practice. We investigate a new dynamic line rating methodology using computational fluid dynamics (CFD) to determine wind conditions along transmission lines at dense intervals. Simulated results are used to determine conductor temperature by calculating the transient thermal response of the conductor under variable environmental conditions. In calculating the conductor temperature, we use both a calculation with steady-state assumption and a transient calculation. Under low wind conditions, steady-state assumption predicts higher conductor temperatures that could lead to curtailments, whereas transient calculations produce conductor temperatures that are significantly lower, implying the availability of additional transmission capacity.

13 HYDRO ENERGY↗
DOE OSTI2014

ON-LINE MONITORING FOR PROCESS CONTROL AND SAFEGUARDING OF RADIOCHEMICAL STREAMS AT SPENT FUEL REPROCESSING PLANTS

The International Atomic Energy Agency (IAEA) has established international safeguards standards for fissionable material at spent nuclear fuel reprocessing plants to ensure that significant quantities of weapons-grade nuclear material are not diverted from these facilities. Currently, methods to verify material control and accountancy (MC&A) at these facilities require time-consuming and resource-intensive destructive assay (DA). Leveraging new on-line non-destructive assay (NDA) techniques in conjunction with the traditional and highly precise DA methods may provide a more timely, cost-effective and resource-efficient means for MC&A verification at such facilities. Pacific Northwest National Laboratory (PNNL) is developing on-line NDA process monitoring technologies, including a spectroscopy-based monitoring system, to potentially reduce the time and resource burden associated with current techniques. The spectroscopic monitor continuously measures chemical compositions of the process streams including actinide metal ions (U, Pu, Np), selected fission products, and major non-radioactive flowsheet chemicals using UV-vis-near infrared and Raman spectroscopy. This paper provides an overview of the methods and reports our on-going efforts to develop and demonstrate the technologies. Our ability to identify material intentionally diverted from a liquid-liquid extraction contactor system was successfully tested using on-line process monitoring as a means to detect the amount of material diverted. A chemical diversion and detection of that diversion, from a solvent extraction scheme was demonstrated using a centrifugal contactor system operating a tributyl phosphate based extraction. A portion of the feed from a counter-current extraction system was diverted while a continuous extraction experiment was underway; the spectroscopic on-line process monitoring system was simultaneously measuring the feed, raffinate and organic products streams. The amount observed to be diverted by on-line spectroscopic process monitoring was in excellent agreement with values based from the known mass of sample directly diverted from system feed solution.

Np↗
DOE OSTI2018

Realization of a Type-II Nodal-Line Semimetal in Mg 3 Bi 2

Nodal-line semimetals (NLSs) represent a new type of topological semimetallic phase beyond Weyl and Dirac semimetals in the sense that they host closed loops or open curves of band degeneracies in the Brillouin zone. Parallel to the classification of type-I and type-II Weyl semimetals, there are two types of NLSs. The type-I NLS phase has been proposed and realized in many compounds, whereas the exotic type-II NLS phase that strongly violates Lorentz symmetry has remained elusive. First-principles calculations show that Mg 3 Bi 2 is a material candidate for the type-II NLS. The band crossing is close to the Fermi level and exhibits the type-II nature of the nodal line in this material. Spin–orbit coupling generates only a small energy gap (≈35 meV) at the nodal points and does not negate the band dispersion of Mg 3 Bi 2 that yields the type-II nodal line. Based on this prediction, Mg 3 Bi 2 single crystals are synthesized and the presence of the type-II nodal lines in the material is confirmed. The angle-resolved photoemission spectroscopy measurements agree well with the first-principles results below the Fermi level and thus strongly suggest Mg 3 Bi 2 as an ideal material platform for studying the as-yet unstudied properties of type-II nodal-line semimetals.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2019

Simulation of Line Profiles of the Krypton Spectra Emitted by the Plasma of Current Sheets

Line profiles of the krypton spectra emitted by the plasma of current sheets are simulated. Current sheets are formed under the impact of magnetic fields with singular X-lines. The spectral line profiles are calculated by the method of the energy matrix diagonalization of an atom in an electric field. The dependence of the behavior of the line profiles on the parameters of an alternating electric field arising inside the plasma of current sheets and the dependence of these profiles on the plasma temperature are investigated. The criteria which allow one to reveal spectral lines serving as the indicators of the appearance of anomalous electric fields inside current sheets are suggested.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2017

Torsional balloon flight line oscillations: Comparison of modelling to flight data

During the EBEX2013 long duration flight the payload was free to rotate in azimuth. The observed azimuth motion consisted of a superposition of full rotations with a period of 10—30 min and oscillatory motion with an amplitude of tens of degrees, average period of 79 s, and period dispersion of 12 s. We interpret the full rotations as induced by slow rotations of the balloon and the shorter period oscillatory motion as due to torsional oscillations of the flight line. We derive the torsional stiffness of the flight line using the bifilar pendulum model and apply it to the flight line of the EBEX2013 payload. We find a torsional spring constant of 36 kg m 2 /s 2 corresponding to a period of 58 s. Here, we conclude that the bifilar model, which accounts for the geometry of the flight line but neglects all material properties, predicts a stiffness and period that are 45% larger and 25% shorter than those observed. It is useful to have a simple, easy to use, coarse approximation for the torsional constant of the flight line.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2013

The PIKfyve–ArPIKfyve–Sac3 triad in human breast cancer: Functional link between elevated Sac3 phosphatase and enhanced proliferation of triple negative cell lines

Highlights: •We assess PAS complex proteins and phosphoinositide levels in breast cancer cells. •Sac3 and ArPIKfyve are markedly elevated in triple-negative breast cancer cells. •Sac3 silencing inhibits proliferation in triple-negative breast cancer cell lines. •Phosphoinositide profiles are altered in breast cancer cells. •This is the first evidence linking high Sac3 with breast cancer cell proliferation. -- Abstract: The phosphoinositide 5-kinase PIKfyve and 5-phosphatase Sac3 are scaffolded by ArPIKfyve in the PIKfyve–ArPIKfyve–Sac3 (PAS) regulatory complex to trigger a unique loop of PtdIns3P–PtdIns(3,5)P{sub 2} synthesis and turnover. Whereas the metabolizing enzymes of the other 3-phosphoinositides have already been implicated in breast cancer, the role of the PAS proteins and the PtdIns3P–PtdIns(3,5)P{sub 2} conversion is unknown. To begin elucidating their roles, in this study we monitored the endogenous levels of the PAS complex proteins in cell lines derived from hormone-receptor positive (MCF7 and T47D) or triple-negative breast cancers (TNBC) (BT20, BT549 and MDA-MB-231) as well as in MCF10A cells derived from non-tumorigenic mastectomy. We report profound upregulation of Sac3 and ArPIKfyve in the triple negative vs. hormone-sensitive breast cancer or non-tumorigenic cells, with BT cell lines showing the highest levels. siRNA-mediated knockdown of Sac3, but not that of PIKfyve, significantly inhibited proliferation of BT20 and BT549 cells. In these cells, knockdown of ArPIKfyve had only a minor effect, consistent with a primary role for Sac3 in TNBC cell proliferation. Intriguingly, steady-state levels of PtdIns(3,5)P{sub 2} in BT20 and T47D cells were similar despite the 6-fold difference in Sac3 levels between these cell lines. However, steady-state levels of PtdIns3P and PtdIns5P, both regulated by the PAS complex, were significantly reduced in BT20 vs. T47D or MCF10A cell lines, consistent with elevated Sac3 affecting directly or indirectly the homeostasis of these lipids in TNBC. Together, our results uncover an unexpected role for Sac3 phosphatase in TNBC cell proliferation. Database analyses, discussed herein, reinforce the involvement of Sac3 in breast cancer pathogenesis.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

A selective splicing variant of hepcidin mRNA in hepatocellular carcinoma cell lines

Hepcidin is a main regulator of iron metabolism, of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of iron by interacting with ferroportin. It is also noted that abnormal iron accumulation is one of the key factors to facilitate promotion and progression of cancer including hepatoma. By RT-PCR/agarose gel electrophoresis of hepcidin mRNA in a hepatocellular carcinoma cell line HLF, a smaller mRNA band was shown in addition to the wild-type hepcidin mRNA. From sequencing analysis, this additional band was a selective splicing variant of hepcidin mRNA lacking exon 2 of HAMP gene, producing the transcript that encodes truncated peptide lacking 20 amino acids at the middle of preprohepcidin. In the present study, we used the digital PCR, because such a small amount of variant mRNA was difficult to quantitate by the conventional RT-PCR amplification. Among seven hepatoma-derived cell lines, six cell lines have significant copy numbers of this variant mRNA, but not in one cell line. In the transient transfection analysis of variant-type hepcidin cDNA, truncated preprohepcidin has a different character comparing with native preprohepcidin: its product is insensitive to digestion, and secreted into the medium as a whole preprohepcidin form without maturation. Loss or reduction of function of HAMP gene by aberrantly splicing may be a suitable phenomenon to obtain the proliferating advantage of hepatoma cells. - Highlights: • An aberrant splicing variant of hepcidin mRNA lacking exon 2 of HAMP gene. • Absolute quantification of hepcidin mRNA by digital PCR amplification. • Hepatoma-derived cell lines have significant copies of variant-type hepcidin mRNA. • Truncated preprohepcidin is secreted from cells without posttranslational cleavage.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2020

Response to a comment on Dessert et al. “The dark matter interpretation of the 3.5 keV line is inconsistent with blank-sky observations”

The dark matter explanation of the 3.5 keV line is strongly disfavored by our work in Dessert et al. (2020). Boyarsky et al. (2004) questions that conclusion: modeling additional background lines is claimed to weaken the limit sufficiently to re-allow a dark matter interpretation. Here, we respond as follows. (1) A more conservative limit is obtained by modeling additional lines; this point appeared in its entirety in our work in Dessert et al. (2020), though we also showed that the inclusion of such lines is not necessary. (2) Despite suggestions in Boyarsky et al. (2004), even the more conservative limits strongly disfavor a decaying dark matter origin of the 3.5 keV line.

79 ASTRONOMY AND ASTROPHYSICS↗