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At least 1,099 records · Page 61

Protein Crystal Growth With the Aid of Microfluidics

Protein crystallography is one of three well-known methods to obtain the structure of proteins. A major rate limiting step in protein crystallography is protein crystal nucleation and growth, which is still largely a process conducted by trial-and-error methods. Many attempts have been made to improve protein crystal growth by performing growth in microgravity. Although the use of microgravity appears to improve crystal quality in some attempts, this method has been inefficient because several reasons: we lack a fundamental understanding of macromolecular crystal growth in general and of the influence of microgravity in particular, we have to start with crystal growth conditions in microgravity based on conditions on the ground and finally the hardware does not allow for experimental iteration without reloading samples on the ground. To partially accommodate the disadvantages of the current hardware, we have used microfluidic technology (Lab-on-a-Chip devices) to design the concept of a more efficient crystallization device, suitable for use on the International Space Station and in high-throughput applications on the ground. The concept and properties of microfluidics, the application design process, and the advances in protein crystal growth hardware will be discussed in this presentation. Some examples of proteins crystallized in the new hardware will be discussed, including the differences between conventional crystallization versus crystallization in microfluidics.

vanderWoerd, Mark↗

Modeling Aircraft Wing Loads from Flight Data Using Neural Networks

Neural networks were used to model wing bending-moment loads, torsion loads, and control surface hinge-moments of the Active Aeroelastic Wing (AAW) aircraft. Accurate loads models are required for the development of control laws designed to increase roll performance through wing twist while not exceeding load limits. Inputs to the model include aircraft rates, accelerations, and control surface positions. Neural networks were chosen to model aircraft loads because they can account for uncharacterized nonlinear effects while retaining the capability to generalize. The accuracy of the neural network models was improved by first developing linear loads models to use as starting points for network training. Neural networks were then trained with flight data for rolls, loaded reversals, wind-up-turns, and individual control surface doublets for load excitation. Generalization was improved by using gain weighting and early stopping. Results are presented for neural network loads models of four wing loads and four control surface hinge moments at Mach 0.90 and an altitude of 15,000 ft. An average model prediction error reduction of 18.6 percent was calculated for the neural network models when compared to the linear models. This paper documents the input data conditioning, input parameter selection, structure, training, and validation of the neural network models.

Allen, Michael J.↗

Active Optics for a Segmented Primary Mirror on a Deep-Space Optical Receiver Antenna (DSORA)

This article investigates the active optical control of segments in the primary mirror to correct for wavefront errors in the Deep-Space Optical Receiver Antenna (DSORA). Although an exact assessment of improvement in signal blur radius cannot be made until a more detailed preliminary structural design is completed, analytical tools are identified for a time when such designs become available. A brief survey of appropriate sensing approaches is given. Since the choice of control algorithm and architecture depends on the particular sensing system used, typical control systems, estimated complexities, and the type of equipment required are discussed. Once specific sensor and actuator systems are chosen, the overall control system can be optimized using methods identified in the literature.

Clymer, B. D.↗

The Effect of Basis Selection on Static and Random Acoustic Response Prediction Using a Nonlinear Modal Simulation

An investigation of the effect of basis selection on geometric nonlinear response prediction using a reduced-order nonlinear modal simulation is presented. The accuracy is dictated by the selection of the basis used to determine the nonlinear modal stiffness. This study considers a suite of available bases including bending modes only, bending and membrane modes, coupled bending and companion modes, and uncoupled bending and companion modes. The nonlinear modal simulation presented is broadly applicable and is demonstrated for nonlinear quasi-static and random acoustic response of flat beam and plate structures with isotropic material properties. Reduced-order analysis predictions are compared with those made using a numerical simulation in physical degrees-of-freedom to quantify the error associated with the selected modal bases. Bending and membrane responses are separately presented to help differentiate the bases.

Rizzi, Stephen A.↗

Simulation of Deployable Composite Structures Based On Mechanics of Structure Genome

In this paper, a simulation method for analyzing deployable composite structures is presented. With a proper material model, effective plate/shell properties of the composites is obtained based on Mechanics of Structure Genome (MSG), and then implemented into a user subroutine UGENS for global structure simulation in ABAQUS. Column bending test (CBT) and composite boom and hub structure are studied for demonstration. A viscoelastic material model with direct integration implementation is adopted in this paper. CBT simulation shows good agreement with experiments during relaxation, while errors are observed when comparing residual deformation. This simulation can be potentially used as a calibration tool for material properties. After CBT simulation, a demonstrative model with a lenticular boom and the hub is created in ABAQUS. Complete process of flattening, coiling, stowage, deploying and recovery is simulated with the viscoelastic material model. Residual deformation of the boom is analyzed.

Multi-scale modeling↗

Feature Acquisition with Imbalanced Training Data

This work considers cost-sensitive feature acquisition that attempts to classify a candidate datapoint from incomplete information. In this task, an agent acquires features of the datapoint using one or more costly diagnostic tests, and eventually ascribes a classification label. A cost function describes both the penalties for feature acquisition, as well as misclassification errors. A common solution is a Cost Sensitive Decision Tree (CSDT), a branching sequence of tests with features acquired at interior decision points and class assignment at the leaves. CSDT's can incorporate a wide range of diagnostic tests and can reflect arbitrary cost structures. They are particularly useful for online applications due to their low computational overhead. In this innovation, CSDT's are applied to cost-sensitive feature acquisition where the goal is to recognize very rare or unique phenomena in real time. Example applications from this domain include four areas. In stream processing, one seeks unique events in a real time data stream that is too large to store. In fault protection, a system must adapt quickly to react to anticipated errors by triggering repair activities or follow- up diagnostics. With real-time sensor networks, one seeks to classify unique, new events as they occur. With observational sciences, a new generation of instrumentation seeks unique events through online analysis of large observational datasets. This work presents a solution based on transfer learning principles that permits principled CSDT learning while exploiting any prior knowledge of the designer to correct both between-class and withinclass imbalance. Training examples are adaptively reweighted based on a decomposition of the data attributes. The result is a new, nonparametric representation that matches the anticipated attribute distribution for the target events.

Thompson, David R.↗

Center of mass estimation in closed vortices - A verification in principle and practice

The problem of tracking closed mesoscale vortices using center of mass estimation techniques is studied. Three estimators are evaluated using data from a warm core Gulf Stream ring. The comparisons show that a method based on the intersection of perpendicular bisectors and one using a least-squares fit of a conic section perform comparably. The perpendicular bisector algorithm is used in conjunction with a Gaussian ring model and a star-shaped survey pattern to produce an expected error curve as a function of vortex translation, survey speed and vortex size. For typical ring parameters, center estimation is usually possible to within + or - 5 km. The feasibility of using differing data sets to construct a history of ring motion based on a coordinate system moving with the ring is also investigated. In this way, the validity of using satellite-derived data and drifter trajectories to estimate the center of mass of a mesoscale feature is assessed. The results of the analysis demonstrate that the location of the deeper structure of the ring and the surface expression are sufficiently well correlated to permit dynamically relevant calculations based on surface measurements. It is shown that satellite-derived data can be used to approximate the center of mass trajectory to within the error in the individual center estimates for the period analyzed. The Lagrangian-drifter-derived centers are offset from the center of mass trajectory in a manner consistent with kinematic arguments.

Hooker, S. B.↗

Prediction of pilot reserve attention capacity during air-to-air target tracking

Reserve attention capacity of a pilot was calculated using a pilot model that allocates exclusive model attention according to the ranking of task urgency functions whose variables are tracking error and error rate. The modeled task consisted of tracking a maneuvering target aircraft both vertically and horizontally, and when possible, performing a diverting side task which was simulated by the precise positioning of an electrical stylus and modeled as a task of constant urgency in the attention allocation algorithm. The urgency of the single loop vertical task is simply the magnitude of the vertical tracking error, while the multiloop horizontal task requires a nonlinear urgency measure of error and error rate terms. Comparison of model results with flight simulation data verified the computed model statistics of tracking error of both axes, lateral and longitudinal stick amplitude and rate, and side task episodes. Full data for the simulation tracking statistics as well as the explicit equations and structure of the urgency function multiaxis pilot model are presented.

Onstott, E. D.↗

Predictions of the structure of turbulent, particle-laden, round jets

Models of gas and particle motion in turbulent, particle-laden, round jets were evaluated using existing measurements of flow structure. Three models were considered: (1) a locally homogeneous flow model, where velocities and turbulent mixing properties of both phases were assumed to be equal; (2) a deterministic separated flow model, where interphase slip was considered but effects of turbulent dispersion were ignored; and (3) a stochastic separated flow model where effects of interphase slip and turbulent dispersion were considered using random sampling techniques. In all three cases, mean and turbulent properties of the continuous phase were found with a well-calibrated k-epsilon model. The locally homogeneous flow and deterministic separated flow models over- and underestimated particle spread and flow development rates, respectively. The stochastic separated flow model, however, yielded satisfactory predictions of flow structure - except at high particle loadings. Uncertainties in initial conditions for the measurements and possible effects of turbulence modulation by the particles are proposed as the reason for these errors.

Shuen, J.-S.↗

Selecting step sizes in sensitivity analysis by finite differences

This paper deals with methods for obtaining near-optimum step sizes for finite difference approximations to first derivatives with particular application to sensitivity analysis. A technique denoted the finite difference (FD) algorithm, previously described in the literature and applicable to one derivative at a time, is extended to the calculation of several simultaneously. Both the original and extended FD algorithms are applied to sensitivity analysis for a data-fitting problem in which derivatives of the coefficients of an interpolation polynomial are calculated with respect to uncertainties in the data. The methods are also applied to sensitivity analysis of the structural response of a finite-element-modeled swept wing. In a previous study, this sensitivity analysis of the swept wing required a time-consuming trial-and-error effort to obtain a suitable step size, but it proved to be a routine application for the extended FD algorithm herein.

Iott, J.↗

Identifiability Of Systems With Modeling Errors

Advances in theory of modeling errors reported. Recent paper on errors in mathematical models of deterministic linear or weakly nonlinear systems. Extends theoretical work described in NPO-16661 and NPO-16785. Presents concrete way of accounting for difference in structure between mathematical model and physical process or system that it represents.

Hadaegh, Yadolah "fred"↗

An experimental evaluation of the effectiveness of random testing of fault-tolerant software

Results of a fault-tolerant software (FTS) experiment are used to show deficiencies of the simple random testing approach. Testing was performed using randomly generated test cases supplemented with extremal and special value (ESV) cases. Error detection efficiency of the random testing approach, with emphasis on correlated errors, was compared to the error detecting capabilities of the ESV data and found deficient. The use of carefully designed test cases as a supplement to random testing, as well as the use of structure based testing are recommended.

Vouk, Mladen A.↗

Performance of concatenated Reed-Solomon trellis-coded modulation over Rician fading channels

A concatenated coding scheme for providing very reliable data over mobile-satellite channels at power levels similar to those used for vocoded speech is described. The outer code is a shorter Reed-Solomon code which provides error detection as well as error correction capabilities. The inner code is a 1-D 8-state trellis code applied independently to both the inphase and quadrature channels. To achieve the full error correction potential of this inner code, the code symbols are multiplexed with a pilot sequence which is used to provide dynamic channel estimation and coherent detection. The implementation structure of this scheme is discussed and its performance is estimated.

Moher, Michael L.↗

Evaluation of piezoceramic actuators for control of aircraft interior noise

Results of an experiment to evaluate piezoceramic actuators as the control actuator for active control of interior noise in a large-scale fuselage model are presented. Control was demonstrated for tonal excitation using a time domain least mean squares algorithm. A maximum of four actuator channels and six error signals were used. The actuators were employed for control of noise at frequencies where interior cavity modes were the dominant response and for driven acoustic responses where a structure resonance was dominant. Global reductions of 9 to 12 dB were obtained for the cases examined. The most effective configuration of skin-mounted actuators was found to be a pure in-plane forcing function as opposed to a bending excitation. The frame-mounted actuators were found to be equally effective as the skin-mounted actuators. However, both configurations resulted in local regions of unacceptably high vibration response in the structure.

Silcox, Richard J.↗

Cammatic: An approach to improve DSS 13 antenna gravity-loading performance

Corrective actuator forces are applied to the DSS 13 antenna structure to improve its accuracy. These forces are calibrated according to the antenna elevation requirements. The calibration is derived from accurate field measurements and is not subject to the errors that result from computer modeling. A successful demonstration provided significant antenna surface improvement. An improvement of 0.6 to 1 dB is predicted.

Levy, R.↗

A passive microwave technique for estimating rainfall and vertical structure information from space. Part 1: Algorithm description

This paper describes a multichannel physical approach for retrieving rainfall and vertical structure information from satellite-based passive microwave observations. The algorithm makes use of statistical inversion techniques based upon theoretically calculated relations between rainfall rates and brightness temperatures. Potential errors introduced into the theoretical calculations by the unknown vertical distribution of hydrometeors are overcome by explicity accounting for diverse hydrometeor profiles. This is accomplished by allowing for a number of different vertical distributions in the theoretical brightness temperature calculations and requiring consistency between the observed and calculated brightness temperatures. This paper will focus primarily on the theoretical aspects of the retrieval algorithm, which includes a procedure used to account for inhomogeneities of the rainfall within the satellite field of view as well as a detailed description of the algorithm as it is applied over both ocean and land surfaces. The residual error between observed and calculated brightness temperatures is found to be an important quantity in assessing the uniqueness of the solution. It is further found that the residual error is a meaningful quantity that can be used to derive expected accuracies from this retrieval technique. Examples comparing the retrieved results as well as the detailed analysis of the algorithm performance under various circumstances are the subject of a companion paper.

Kummerow, Christian↗

Application of the coded long-pulse technique to plasma line studies of the ionosphere

Recently, the coded long-pulse radar technique was tested at Arecibo Observatory, Puerto Rico using photoelectron-enhanced plasma lines in the daytime ionosphere. The technique immediately proved to be a powerful diagnostic tool for studying natural ionospheric phenomena. Our initial observations indicate that extremely accurate measurements of absolute electron density (0.01 to 0.03% error bars) can be achieved with an altitude resolution of 150 m and a temporal resolution of approx. 2 s. In addition, the technique provides information about electron density structure within a 150-m altitude cell and yields parameters from which the energy spectrum of suprathermal electrons (equal to or greater than 5 eV) can be deduced. Our earliest measurements are used to illustrate applications of the coded long-pulse technique to several aeronomic/ionsospheric areas of current interest. These include studies of neutral wave motions in the lower thermosphere, measurements of ion composition in the F(sub 1) region/upper ionosphere, and investigations of electron-gas thermal balance and photoelectron energy loss processes. The technique can be utilized to examine irregularity formation in the F region, probe electron acceleration processes in ionospheric modification experiments, verify the magnetic field dependence of Langmuir wave damping, and more generally test higher order corrections suggested for the Langmuir dispersion relation. It is anticipated that the latter tests will facilitate measurements of ionospheric currents.

Djuth, Frank T.↗