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At least 1,153 records · Page 64

Effects of calcium gluconate on lipopolysaccharide-induced acute lung injury in mice

Highlights: • Calcium gluconate may decrease lipopolysaccharide-induced lung histological changes. • Exudation of neutrophils could be relieved by calcium gluconate. • Inflammatory cytokines release in airways could be reduced by calcium gluconate. • Calcium gluconate could inhibit the ERK phosphorylation. Lipopolysaccharide (LPS), which can cause acute airway inflammatory reactions, constitutes one of the most common substances to establish acute lung injury (ALI) models in mice. Studies suggest that calcium gluconate offers the possibility of suppressing the immune response, and this study was intended to explore the effects of calcium gluconate on LPS-induced ALI in mice. Mice inhaled with LPS were intraperitoneally injected with calcium gluconate (12.5, 25, 50 mg/kg). IL-1β, IL-6 and TNF-α levels in bronchoalveolar lavage fluid (BALF) were determined by ELISA. The expression of signaling proteins, phosphorylation extracellular regulated protein kinases (p-ERK), was detected using Western Blot in lung tissues. In our study, the release of inflammatory cytokines IL-1β, IL-6 and TNF-α in BALF increased after inhalation of LPS. Post-treatment with calcium gluconate inhibited LPS-induced airway inflammatory injury and the release of inflammatory cytokines. In addition, LPS promoted the expression of signaling protein p-ERK while calcium gluconate was capable of reversing this change. Overall, calcium gluconate inhibits LPS-induced ALI in mice, which may take effects through the inhibition of ERK phosphorylation.

60 APPLIED LIFE SCIENCES↗

On the emergence of a 1/k spectrum in the sub-inertial domain of turbulent media

Several physical and astrophysical fluid and plasma systems displaying turbulent features show a 1/k (or 1/f) spectral domain at spatial (temporal) scales above the inertial range. Here, we investigate the formation of a 1/k spectral domain in fluid and MHD turbulence systems in the sub-inertial range (k<k{sub 0}, where k{sub 0} is the injection scale) by means of shell-model simulations. The simulation results suggest that the 1/k domain emerges as a consequence of competing direct and inverse cascading processes, implying the formation and dissipation of large-scale gradients below the inertial domain. The relevance of our results for observations in space and astrophysical contexts is briefly discussed.

79 ASTRONOMY AND ASTROPHYSICS↗

Understanding the depletion of electrons in dusty plasmas at atmospheric pressure

The nucleation and growth of NPs in the gas phase using atmospheric-pressure plasma systems is an important approach to synthesizing novel dimensionally-controlled materials. In this paper, we investigated the effect of the NPs on a typical type of continuous-flow, substrate-free plasma at atmospheric pressure to understand their potential contribution to electron density changes. A tandem plasma system was set up consisting of one plasma reactor that produced carbonaceous NPs from mixtures of argon and hexane, and another identical plasma reactor where the as-grown particles were injected and non-intrusive electrical and optical measurements were performed. The electron densities obtained from conductivity measurements and a plasma fluid model were found to decrease in the presence of NPs. However, control experiments revealed that the main source of the electron depletion was residual vapor or reaction byproducts in the form of molecular species or nanoclusters and not the particles themselves. These results were validated by constant number Monte Carlo simulations which showed that at the experimentally-measured conditions, the NPs were not of sufficiently high enough concentration to reduce the electron density; however, if molecules or clusters are ionizable, they remain in sufficient concentration to deplete electron densities. Our study shows that at atmospheric pressure, because of their typically larger electron density values, particle-producing plasmas are distinct from those at low pressure, and nanoparticle formation does not have the same impact while molecular-scale species may be a more important consideration.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Role of Inlet Boundary Conditions on Fuel-Air Mixing at Supercritical Conditions

Advanced engine design and alternative fuels present the possibility of fuel injection at purely supercritical conditions in diesel engines and gas turbines. The complex interactions that govern this phenomenon still need significant research, particularly the boundary conditions for fuel injection are critical for accurate simulation. However, the flow inside the injector itself is often omitted to reduce the computational efforts, and thus, velocity, mass flux, or total pressure is specified at the injector exit (or domain inlet), often with simplified velocity profiles and turbulence levels. This simplified inlet boundary treatment has minimal effects on results for conventional fuel injection conditions, however, the validity of this approach at supercritical conditions has not been assessed. Comprehensive real-gas and binary fluid mixing models have been implemented for computational fluid dynamics (CFD) analysis of fuel-air mixing at supercritical conditions. The model is verified using prior CFD results from the literature. The model is used to investigate the effects of the shape of axial velocity and mass fraction profiles at the inlet boundary with the goal to improve the comparison of predictions to experimental data. Results show that the boundary conditions have a significant effect on the predictions, and none of the cases match precisely with experimental data. Furthermore, the study reveals that the physical location of the inlet boundary might be difficult to infer correctly from the experiments and highlights the need for high-quality, repeatable measurements at supercritical conditions to support the development of relevant high-fidelity models for fuel-air mixing.

02 PETROLEUM↗

Computational Analysis of the Impact of Boundary Conditions on a Particle-Laden Flow: A Case Study in a Pressurized Oxy-Coal Combustor

Designing an effective burner is vital for the development of coal combustion technologies. Because of high pressure, the volumetric fraction of the coal particles in the injected fuel in a pressurized oxy-combustion (POC) burner approaches or even exceeds the limitations allowed by the commercial computational fluid dynamics codes (e.g., Ansys Fluent). Consequently, for such high particle volumetric fractions, the interplay between the particles, the fluid flow, and the burner wall needs to be re-evaluated. The present computational work is a first step in a systematic analysis of the roles of various characteristics involved in the POC process, such as the method of particle release, its location, and the particle size. Specifically, pulverized coal is burned under an elevated pressure of 15 bar in an O 2 /CO 2 environment. A 100 kW, a POC combustor, is modeled with Ansys Fluent using the Reynolds-averaged Navier−Stokes approach. It is revealed that for this pilot-scale, pressurized burner, the gas phase flow velocity in the near-wall region exhibits anomalies. With the major focus on POC, this work aims to eliminate/reduce the impact of high particle loading on the gas-phase flow. To scrutinize the role of particle loading in the near-wall region and eliminate the impact of this velocity on POC downstream, the particle−gas interplay in the boundary layer is investigated by means of the computational simulations incorporating the coupling between the turbulent flow and the particles. It is found that the tuning of the particle release location makes the gas-phase flow velocity in the presence of particles consistent with the pure gas flow velocity profile. The particles size is also found to have a significant impact on the particle trajectory.

CFD↗

Computational Modeling of Proppants Transport in Rock Fractures

Presentation describing the direct numerical simulations (DNS) of fluid and particulate transport through rough-walled rock fractures showing transport properties of proppants as a function of fracture properties, injection velocity, and proppant size. Results demonstrate the particulate transport is highly influenced by the ratio of proppant diameter to mean fracture aperture, and quantified in terms of areal space that proppant coverage occurs.

Rousta, Farid↗

Analysis of Skylab IV fluid mechanic science demonstration

Several science demonstrations performed on Skylab III and IV were concerned with the behavior of fluid drops free floating in microgravity. These demonstrations, with large liquid drops, included the oscillation, rotation, impact and coalescence, and air injection into the drops. Rayleigh's analysis of the oscillation of spherical drops of a liquid predicts accurately the effect of size and surface tension on the frequency of vibrated water globules in the Skylab demonstration. However, damping occurred much faster than predicted by Lamb's or Scriven's analyses of the damping time for spherical drops. The impact demonstrations indicated that a minimum velocity is necessary to overcome surface forces and effect a coalescence, but a precise criterion for the coalescence of liquids in low g could not be determined.

Klett, M. G.↗

Plasma core reactor applications

Analytical and experimental investigations were conducted to demonstrate the feasibility of fissioning uranium plasma core reactors and to characterize space and terrestrial applications for such reactors. Uranium hexafluoride fuel is injected into core cavities and confined away from the surface by argon buffer gas injected tangentially from the peripheral walls. Radiant heat transfer calculations were performed for a six-cavity reactor configuration. Axial working fluid channels are located along a fraction of each cavity peripheral wall. Results of calculations for outward-directed radiant energy fluxes corresponding to radiating temperatures of 2000 to 5000 K indicate total operating pressures from 80 to 650 atm, centerline temperatures from 6900 to 30,000 K, and total radiated powers from 25 to 2500 MW, respectively. Applications are described for this type of reactor such as (1) high-thrust, high specific impulse space propulsion, (2) highly efficient systems for generation of electricity, and (3) hydrogen or synthetic fuel production systems using the intense radiant energy fluxes.

Latham, T. S.↗

Supercritical fuel injection system

a fuel injection system for gas turbines is described including a pair of high pressure pumps. The pumps provide fuel and a carrier fluid such as air at pressures above the critical pressure of the fuel. A supercritical mixing chamber mixes the fuel and carrier fluid and the mixture is sprayed into a combustion chamber. The use of fuel and a carrier fluid at supercritical pressures promotes rapid mixing of the fuel in the combustion chamber so as to reduce the formation of pollutants and promote cleaner burning.

Marek, C. J.↗

Fluid flow and fuel-air mixing in a motored two-dimensional Wankel rotary engine

The implicit-factored method of Beam and Warming was employed to obtain numerical solutions to the conservation equations of mass, species, momentum, and energy to study the unsteady, multidimensional flow and mixing of fuel and air inside the combustion chambers of a two-dimensional Wankel rotary engine under motored conditions. The effects of the following engine design and operating parameters on fluid flow and fuel-air mixing during the intake and compression cycles were studied: engine speed, angle of gaseous fuel injection during compression cycle, and speed of the fuel leaving fuel injector.

Shih, T. I.-P.↗

In-flight flow visualization with pressure measurements at low speeds on the NASA F-18 high alpha research vehicle

In-flight results from surface and off-surface flow visualizations and from extensive pressure distributions document the vortical flow on the leading edge extensions (LEX) and forebody of the NASA F-18 high alpha research vehicle for low speeds and angles of attack up to 50 degs. Surface flow visualization data, obtained using the emitted fluid technique, were used to define separation lines and laminar separation bubbles. Off-surface flow visualization data, obtained by smoke injection, were used to document both the path of the vortex cores and the location of vortex core breakdown. The location of vortex core breakdown correlated well with the loss of suction pressure on the LEX and with the flow visualization results from ground facilities. Surface flow separation lines on the LEX and forebody corresponded well with the end of pressure recovery under the vortical flows. Correlation of the pressures with wind tunnel results show fair to good correlation.

Delfrate, John H.↗

In-flight flow visualization and pressure measurements at low speeds on the NASA F-18 high alpha research vehicle

Inflight results from surface and off surface flow visualizations and from extensive pressure distributions document the vortical flow on the leading edge extensions (LEXs) and forebody of the NASA F-18 high alpha research vehicle (HARV) for low speeds and angles of attack up to 50 deg. Surface flow visualization data, obtained using the emitted fluid technique, were used to define separation lines and laminar separation bubbles (LSB). Off surface flow visualization data, obtained by smoke injection, were used to document both the path of the vortex cores and the location of vortex core breakdown. The location of vortex core breakdown correlated well with the loss of suction pressure on the LEX and with the flow visualization results from ground facilities. Surface flow separation lines on the LEX and forebody corresponded well with the end of pressure recovery under the vortical flows. Correlation of the pressures with wind tunnel results show fair to good correlation.

John H Del Frate↗

Development of Advanced Casing Treatments for Flow Control

Under the Base R&T and Ultra Efficient Engine Technology programs, the NASA-Goddard Space Flight Center Compressor Branch is investigating flow control strategies required to increase the loading and efficiency of core compressors while maintaining current levels of operability. Flow-control strategies being studied include advanced casing treatments, wall jet injection, and blade-tip injection for compressor stability enhancement, directed jets for surface boundary layer control, and vortex-generating devices. The use of computational fluid dynamics (CFD) simulations to assess the effectiveness of flow-control devices and to guide their design is a key element in this research. CFD simulations serve to screen potential flow-control concepts at a lower cost than executing physical experiments in turbomachinery facilities. CFD simulations also provide guidance in designing physical experiments for those flow control concepts, which appear promising.

Keith, Theo G., Jr.↗

Ducted combustion systems utilizing flow field preparation

A ducted combustion system includes a combustion chamber and a fuel injector in fluid connection with the combustion chamber, which includes an orifice opening from an injector tip of the fuel injector, the orifice injecting fuel into the combustion chamber as a fuel jet, the fuel jet flowing, within the combustion chamber, in a fuel flow direction. The system includes at least one duct disposed within the combustion chamber, the at least one duct being disposed such that the fuel jet, at least partially, enters one of the at least one ducts upon being injected into the combustion chamber. The at least one duct may be configured for having a flow field air stream within the duct, prior to entrance of the fuel jet, the flow field air stream having a flow direction that is substantially similar to the fuel flow direction.

42 ENGINEERING↗

Application of Machine Learning to Predict the Response of the Liquid Mercury Target at the Spallation Neutron Source

The Spallation Neutron Source (SNS) at Oak Ridge National Laboratory is currently the most powerful accelerator-driven neutron source in the world. The intense proton pulses strike on SNS’s mercury target to provide bright neutron beams, which also leads to severe fluid-structure interactions inside the target. Prediction of resultant loading on the target is difficult particularly when helium gas is intentionally injected into mercury to reduce the loading and mitigate the pitting damage on the target’s internal walls. Leveraging the power of machine learning and the measured target strain, we have developed machine learning surrogates for modeling the discrepancy between simulations and experimental strain data. We then employ these surrogates to guide the refinement of the high-fidelity mercury/helium mixture model to predict a better match of target strain response.

Lin, Lianshan↗

Evaluation of Shot-to-Shot In-Nozzle Flow Variations in a Heavy-Duty Diesel Injector Using Real Nozzle Geometry

Cyclic variability in internal combustion engines (ICEs) arises from multiple concurrent sources, many of which remain to be fully understood and controlled. This variability can, in turn, affect the behavior of the engine resulting in undesirable deviations from the expected operating conditions and performance. Shot-to-shot variation during the fuel injection process is strongly suspected of being a source of cyclic variability. This study focuses on the shot-to-shot variability of injector needle motion and its influence on the internal nozzle flow behavior using diesel fuel. High-speed x-ray imaging techniques have been used to extract high-resolution injector geometry images of the sac, orifices, and needle tip that allowed the true dynamics of the needle motion to emerge. These measurements showed high repeatability in the needle lift profile across multiple injection events, while the needle radial displacement was characterized by a much higher degree of randomness. A robust and previously validated computational setup from the authors’ research group using a commercial computational fluid dynamics (CFD) code has been adapted for the eight-hole heavy-duty common-rail diesel injector used for the measurements. The simulation results obtained using the x-ray-scanned geometry have been validated against available experimental data of mass flow rate at the nozzle exit. The fuel mass flow rate has then been analyzed at three different injection pressures that cover the conditions at which the injector typically operates during normal engine operation. Finally, the average off-axis motion of the needle has been perturbed (based on the variability found in the experimental measurements) to generate three new cases that present different amplitude and phasing of the radial displacement with respect to the baseline average motion. Furthermore, this revealed the effects of off-axis motion on shot-to-shot and orifice-to-orifice variations.

42 ENGINEERING↗

Fluid-kinetic modeling of a high power density radio frequency inductively coupled positive hydrogen ion source

High power density radio-frequency (RF) inductively coupled positive ion sources are attractive candidates for next-generation neutral beam injection (NBI) systems, where higher injected power and longer pulse lengths are desired without sacrificing source reliability. Operating at absorbed power densities of order $\gt 1~\mathrm{W\,cm}^{-3}$ places these sources in a regime with stronger gas heating, higher dissociation, and non-Maxwellian electron energy distributions. The Large Uniform Plasma for Ionizing Neutrals (LUPIN) is an RF inductively coupled plasma source designed to explore this high power density regime and to provide guidance for a positive ion source upgrade for the DIII-D NBI system. LUPIN is designed to operate at up to 20 kW of RF power at 2 MHz, coupling energy through a cylindrical quartz vessel to achieve target ion current densities of $2100\,\mathrm{A\,m}^{-2}$ . This paper presents fluid-kinetic modeling of LUPIN using the hybrid plasma equipment model where electrons are treated kinetically, and the simulations reveal that electron energy distribution function transitions from nearly Maxwellian in the core to bi-Maxwellian towards the edge. Parametric simulations investigate the effects of RF power, gas pressure, and frequency on plasma density, ion flux, and uniformity. Parametric sweeps reveal that increasing power shifts the primary ionization channel from molecular to atomic with diminishing flux gains due to skin-depth contraction and gas rarefaction. Higher frequency localizes heating and increases $\mathrm{H}_2^+$ and $\mathrm{H}_3^+$ delivery to the grid, while elevated pressure boosts ionization yet hinders ion transport due to increase in collisionality.

inductively coupled plasma↗

Seismic monitoring of a small CO 2 injection using a multi-well DAS array: Operations and initial results of Stage 3 of the CO 2 CRC Otway project

Active time-lapse seismic is widely employed for monitoring CO 2 geosequestration due to its ability to track the distribution of fluids in space and time. However, standard 4D seismic monitoring suffers from several challenges, including high cost, disruption to other land uses, and, consequently, relatively large intervals between monitor surveys. Some of these challenges can be mitigated using permanently installed sources and receivers. Such an approach was tested at the CO 2 CRC Otway site by continuous offset VSP monitoring of 15,000 t of supercritical CO 2 injected into an aquifer 1,500 m deep with nine permanent seismic sources (surface orbital vibrators or SOVs) and five downhole fibre-optic receivers. This continuous monitoring is complemented by multi-well 4D VSP using a mobile vibroseis source and the same DAS receivers, which included one baseline and two monitor surveys after injection of 4,000 and 12,000 t of CO 2 . The continuous DAS-SOV monitoring detected an abrupt increase of travel times below the injection interval on the second day of injection (after injection of 300 t of CO 2 ) and tracked the growth of the areal CO 2 plume by mapping changes of reflection amplitudes. The plume is also detected by time-lapse changes of reflection amplitudes in multi-well 4D VSPs. The plume images obtained from continuous offset VSP and 4D VSP are broadly consistent with each other but with some differences due to differences in illumination, lateral variations of velocities and seismic anisotropy. Furthermore, these differences also serve as a measure of uncertainty of 4D VSP images.

4D VSP↗