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At least 1,153 records · Page 64

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NASA NTRS2010

Daytime Water Detection Based on Color Variation

Robust water detection is a critical perception requirement for unmanned ground vehicle (UGV) autonomous navigation. This is particularly true in wide open areas where water can collect in naturally occurring terrain depressions during periods of heavy precipitation and form large water bodies (such as ponds). At far range, reflections of the sky provide a strong cue for water. But at close range, the color coming out of a water body dominates sky reflections and the water cue from sky reflections is of marginal use. We model this behavior by using water body intensity data from multiple frames of RGB imagery to estimate the total reflection coefficient contribution from surface reflections and the combination of all other factors. Then we describe an algorithm that uses one of the color cameras in a forward- looking, UGV-mounted stereo-vision perception system to detect water bodies in wide open areas. This detector exploits the knowledge that the change in saturation-to-brightness ratio across a water body from the leading to trailing edge is uniform and distinct from other terrain types. In test sequences approaching a pond under clear, overcast, and cloudy sky conditions, the true positive and false negative water detection rates were (95.76%, 96.71%, 98.77%) and (0.45%, 0.60%, 0.62%), respectively. This software has been integrated on an experimental unmanned vehicle and field tested at Ft. Indiantown Gap, PA.

reflection coefficient↗

DOE OSTI2016

A hybrid approach to estimate the complex motions of clouds in sky images

Tracking the motion of clouds is essential to forecasting the weather and to predicting the short-term solar energy generation. Existing techniques mainly fall into two categories: variational optical flow, and block matching. In this article, we summarize recent advances in estimating cloud motion using ground-based sky imagers and quantitatively evaluate state-of-the-art approaches. Then we propose a hybrid tracking framework to incorporate the strength of both block matching and optical flow models. To validate the accuracy of the proposed approach, we introduce a series of synthetic images to simulate the cloud movement and deformation, and thereafter comprehensively compare our hybrid approach with several representative tracking algorithms over both simulated and real images collected from various sites/imagers. The results show that our hybrid approach outperforms state-of-the-art models by reducing at least 30% motion estimation errors compared with the ground-truth motions in most of simulated image sequences. Furthermore, our hybrid model demonstrates its superior efficiency in several real cloud image datasets by lowering at least 15% Mean Absolute Error (MAE) between predicted images and ground-truth images.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2015

A PILOT DEEP SURVEY FOR X-RAY EMISSION FROM fuvAGB STARS

We report the results of a pilot survey for X-ray emission from a newly discovered class of AGB stars with far-ultraviolet excesses (fuvAGB stars) using XMM-Newton and Chandra. We detected X-ray emission in three of six fuvAGB stars observed—the X-ray fluxes are found to vary in a stochastic or quasi-periodic manner on roughly hour-long timescales, and simultaneous UV observations using the Optical Monitor on XMM for these sources show similar variations in the UV flux. These data, together with previous studies, show that X-ray emission is found only in fuvAGB stars. From modeling the spectra, we find that the observed X-ray luminosities are ∼(0.002–0.2) L{sub ⊙} and the X-ray-emitting plasma temperatures are ∼(35–160) × 10{sup 6} K. The high X-ray temperatures argue against the emission arising in stellar coronae, or directly in an accretion shock, unless it occurs on a WD companion. However, none of the detected objects is a known WD-symbiotic star, suggesting that if WD companions are present, they are relatively cool (<20,000 K). In addition, the high X-ray luminosities specifically argue against emission originating in the coronae of main-sequence companions. We discuss several models for the X-ray emission and its variability and find that the most likely scenario for the origin of the X-ray (and FUV) emission involves accretion activity around a companion star, with confinement by strong magnetic fields associated with the companion and/or an accretion disk around it.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2024

Stress Field Dynamics and Fault Slip Potential in the Paradox Basin

Abstract The Paradox Basin, straddling Utah, Colorado, Arizona, and New Mexico is characterized by an intricate amalgamation of evaporites and clastic layers and is dominated by prominent salt walls and related subsurface structures. Our research offers a new examination of the stress distribution across the basin, deriving from continuous and discrete stress measurements conducted in boreholes in the region and focal mechanism analysis, emphasizing variations over salt structures. Integrating Coulomb failure criteria with probabilistic methods, we assess potential fault movements resulting from fluid pressure alterations. Our approach provides a comprehensive understanding of the Paradox Basin's state of stress, showing a continuous change of the maximum horizontal stress orientation from N‐S at the Wasatch Fault Zone to WNW‐ESE in the northern part of the Paradox Basin and to WSW‐ENE in the southern part of the basin. Further East, into the Colorado Plateau and the Uncompahgre Uplift, the S H max orientation becomes E‐W. Decoding stress orientation dynamics has enabled critical insights into fault slip potential, especially in the basin's northern region. The salt wall faults are less likely to slip, and the Paradox Formation's evaporite and clastic rock sequence can serve as a potential low seismic risk target for carbon storage and hydrocarbon extraction.

Geochemistry & Geophysics↗

DOE OSTI2015

Polymorphic SSR markers for Plasmopara obducens (Peronosporaceae), the newly emergent downy mildew pathogen of Impatiens (Balsaminaceae)

Premise of the study: Simple sequence repeat (SSR) markers were developed for Plasmopara obducens , the causal agent of the newly emergent downy mildew disease of Impatiens walleriana . Methods and Results: A 202‐Mb draft genome assembly was generated from P. obducens using Illumina technology and mined to identify 13,483 SSR motifs. Primers were synthesized for 62 marker candidates, of which 37 generated reliable PCR products. Testing of the 37 markers using 96 P. obducens samples showed 96% of the markers were polymorphic, with 2−6 alleles observed. Observed and expected heterozygosity ranged from 0.000−0.892 and 0.023−0.746, respectively. Just 17 markers were sufficient to identify all multilocus genotypes. Conclusions: These are the first SSR markers available for this pathogen, and one of the first molecular resources. These markers will be useful in assessing variation in pathogen populations and determining the factors contributing to the emergence of destructive impatiens downy mildew disease.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1994

Accuracy assessment of the large-scale dynamic ocean topography from TOPEX/POSEIDON altimetry

The quality of TOPEX/POSEIDON determinations of the global scale dynamic ocean topography have been assessed by determining mean topography solutions for successive 10-day repeat cycles and by examining the temporal changes in the sea surface topography to identify known features. The assessment is based on the analysis of TOPEX altimeter data cycles 1 through 36. Important errors in the tide model used to correct the altimeter data have been identified. The errors were reduced significantly by use of a new tide model derived with the TOPEX/POSEIDON measurements. Maps of the global 1-year mean topography, produced using four of the most accurate of the marine geoid, show that the largest error in the dynamic ocean topography show expected features, such as the known annual hemispherical sea surface rise and fall and the seasonal variability due to monsoon influence in the Indian Ocean. Changes in the sequence of 10-day topography maps show the development and propagation of an equatorial Kelvin wave in the Pacific beginning in December 1992 with a propagation velocity of approximately 3 m/s. The observations are consistent with observed changes in the equatorial trade winds, and with tide gauge and other in situ observations of the strengthening of the El Nino. Comparison of TOPEX-determine sea surface height at points near oceanic tide gauges shows agreement at the 4 cm root-mean-square (RMS) level over the tropical Pacific. The results show that the TOPEX altimeter data set can be used to map the ocean surface with a temporal resolution of 10 days and an accuracy which is insonsistent with traditional in situ methods for the determination of sea level variations.

Tapley, B. D.↗

NASA NTRS2019

WASP-4b Arrived Early for the TESS Mission

The Transiting Exoplanet Survey Satellite (TESS) recently observed 18 transits of the hot Jupiter WASP-4b. The sequence of transits occurred 81.6±11.7 s earlier than had been predicted, based on data stretching back to 2007.This is unlikely to be the result of a clock error, because TESS observations of other hot Jupiters (WASP-6b, 18b,and 46b) are compatible with a constant period, ruling out an 81.6 s offset at the 6.4σlevel. The 1.3 day orbital period of WASP-4b appears to be decreasing at a rate of P12.6 1.2= ms per year. The apparent period change might be caused by tidal orbital decay or apsidal precession, although both interpretations have shortcomings. The gravitational influence of a third body is another possibility, though at present there is minimal evidence for such a body. Further observations are needed to confirm and understand the timing variation.

Binaries↗

NASA NTRS1998

Segmentation and tracking in echocardiographic sequences: active contours guided by optical flow estimates

This paper presents a method for segmentation and tracking of cardiac structures in ultrasound image sequences. The developed algorithm is based on the active contour framework. This approach requires initial placement of the contour close to the desired position in the image, usually an object outline. Best contour shape and position are then calculated, assuming that at this configuration a global energy function, associated with a contour, attains its minimum. Active contours can be used for tracking by selecting a solution from a previous frame as an initial position in a present frame. Such an approach, however, fails for large displacements of the object of interest. This paper presents a technique that incorporates the information on pixel velocities (optical flow) into the estimate of initial contour to enable tracking of fast-moving objects. The algorithm was tested on several ultrasound image sequences, each covering one complete cardiac cycle. The contour successfully tracked boundaries of mitral valve leaflets, aortic root and endocardial borders of the left ventricle. The algorithm-generated outlines were compared against manual tracings by expert physicians. The automated method resulted in contours that were within the boundaries of intraobserver variability.

Non-NASA Center↗

DOE OSTI2023

The little skate genome and the evolutionary emergence of wing-like fins

Skates are cartilaginous fish whose body plan features enlarged wing-like pectoral fins, enabling them to thrive in benthic environments. However, the molecular underpinnings of this unique trait remain unclear. Here we investigate the origin of this phenotypic innovation by developing the little skate Leucoraja erinacea as a genomically enabled model. Analysis of a high-quality chromosome-scale genome sequence for the little skate shows that it preserves many ancestral jawed vertebrate features compared with other sequenced genomes, including numerous ancient microchromosomes. Combining genome comparisons with extensive regulatory datasets in developing fins—including gene expression, chromatin occupancy and three-dimensional conformation—we find skate-specific genomic rearrangements that alter the three-dimensional regulatory landscape of genes that are involved in the planar cell polarity pathway. Functional inhibition of planar cell polarity signalling resulted in a reduction in anterior fin size, confirming that this pathway is a major contributor to batoid fin morphology. We also identified a fin-specific enhancer that interacts with several hoxa genes, consistent with the redeployment of hox gene expression in anterior pectoral fins, and confirmed its potential to activate transcription in the anterior fin using zebrafish reporter assays. Our findings underscore the central role of genome reorganization and regulatory variation in the evolution of phenotypes, shedding light on the molecular origin of an enigmatic trait.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2018

Structure of the 30S ribosomal decoding complex at ambient temperature

The ribosome translates nucleotide sequences of messenger RNA to proteins through selection of cognate transfer RNA according to the genetic code. To date, structural studies of ribosomal decoding complexes yielding high-resolution data have predominantly relied on experiments performed at cryogenic temperatures. New light sources like the X-ray free electron laser (XFEL) have enabled data collection from macromolecular crystals at ambient temperature. Here, we report an X-ray crystal structure of the Thermus thermophilus 30S ribosomal subunit decoding complex to 3.45 Å resolution using data obtained at ambient temperature at the Linac Coherent Light Source (LCLS). We find that this ambient-temperature structure is largely consistent with existing cryogenic-temperature crystal structures, with key residues of the decoding complex exhibiting similar conformations, including adenosine residues 1492 and 1493. Minor variations were observed, namely an alternate conformation of cytosine 1397 near the mRNA channel and the A-site. Our serial crystallography experiment illustrates the amenability of ribosomal microcrystals to routine structural studies at ambient temperature, thus overcoming a long-standing experimental limitation to structural studies of RNA and RNA–protein complexes at near-physiological temperatures.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1975

Theoretical studies of massive stars. I - Evolution of a 15-solar-mass star from the zero-age main sequence to neon ignition

The evolution of a star with mass 15 times that of the sun from the zero-age main sequence to neon ignition has been computed by the Henyey method. The hydrogen-rich envelope and all shell sources were explicitly included in the models. An algorithm has been developed for approximating the results of carbon burning, including the branching ratio for the C-12 + C-12 reaction and taking some secondary reactions into account. Penetration of the convective envelope into the core is found to be unimportant during the stages covered by the models. Energy transfer from the carbon-burning shell to the core by degenerate electron conduction becomes important after the core carbon-burning stage. Neon ignition will occur in a semidegenerate core and will lead to a mild 'flash.' Detailed numerical results are given in an appendix. Continuation of the calculations into later stages and variations with the total mass of the star will be discussed in later papers.

Endal, A. S.↗

NASA NTRS2004

Exceptional Stars Origins, Companions, Masses and Planets

As SIM Interdisciplinary Scientist, we will study the formation, nature and planetary companions of the exotic endpoints of stellar evolution. Our science begins with stars evolving from asymptotic branch giants into white dwarfs. We will determine the parallax and orbital inclination of several iron-deficient post-AGB stars, who peculiar abundances and infrared excesses are evidence that they are accreting gas depleted of dust from a circumbinary disk. Measurement of the orbital inclination, companion mass arid parallax will provide critical constraints. One of these stars is a prime candidate for trying nulling observations, which should reveal light reflected from both the circumbinary and Roche disks. The circumbinary disks seem favorable sites for planet formation. Next, we will search for planets around white dwarfs, both survivors froni the main-sequence stage, and ones newly formed from the circumbinary disks of post-AGB binaries or in white dwarf mergers. Moving up in mass, we will measure the orbital reflex of OB/Be companions to pulsars, determine natal kicks and presupernova orbits, and expand the sample of well-determined neutron star masses. We will obtain the parallax of a transient X-ray binary, whose quiescent emission may be thermal emission from the neutron star, aiming for precise measurement of the neutron star radius. Finally, black holes. We will measure the reflex motions of the companion of what appear to be the most massive stellar black holes. The visual orbits will determine natal kicks, and test the assumptions underlying mass estimates made from the radial velocity curves, projected rotation, and ellipsoidal variations. In addition, we will attempt to observe the visual orbit of SS 433, as well as the proper motion of the emission line clumps in its relativistic jets. Additional information is included in the original document.

Kulkarni, Shrinivas R.↗

DOE OSTI2019

Spatial variations in the Milky Way disc metallicity–age relation

ABSTRACT Stellar ages are a crucial component to studying the evolution of the Milky Way. Using Gaia DR2 distance estimates, it is now possible to estimate stellar ages for a larger volume of evolved stars through isochrone matching. This work presents [M/H]–age and [α/M]–age relations derived for different spatial locations in the Milky Way disc. These relations are derived by hierarchically modelling the star formation history of stars within a given chemical abundance bin. For the first time, we directly observe that significant variation is apparent in the [M/H]–age relation as a function of both Galactocentric radius and distance from the disc mid-plane. The [M/H]–age relations support claims that radial migration has a significant effect in the plane of the disc. Using the [M/H] bin with the youngest mean age at each radial zone in the plane of the disc, the present-day metallicity gradient is measured to be −0.059 ± 0.010 dex kpc−1, in agreement with Cepheids and young field stars. We find a vertically flared distribution of young stars in the outer disc, confirming predictions of models and previous observations. The mean age of the [M/H]–[α/M] distribution of the solar neighbourhood suggests that the high-[M/H] stars are not an evolutionary extension of the low-α sequence. Our observational results are important constraints to Galactic simulations and models of chemical evolution.

Feuillet, Diane K.↗

DOE OSTI2020

The mole genome reveals regulatory rearrangements associated with adaptive intersexuality

Linking genomic variation to phenotypical traits remains a major challenge in evolutionary genetics. In this study, we use phylogenomic strategies to investigate a distinctive trait among mammals: the development of masculinizing ovotestes in female moles. By combining a chromosome-scale genome assembly of the Iberian mole, Talpa occidentalis, with transcriptomic, epigenetic, and chromatin interaction datasets, we identify rearrangements altering the regulatory landscape of genes with distinct gonadal expression patterns. These include a tandem triplication involving CYP17A1, a gene controlling androgen synthesis, and an intrachromosomal inversion involving the pro-testicular growth factor gene FGF9, which is heterochronically expressed in mole ovotestes. Transgenic mice with a knock-in mole CYP17A1 enhancer or overexpressing FGF9 showed phenotypes recapitulating mole sexual features. Our results highlight how integrative genomic approaches can reveal the phenotypic impact of noncoding sequence changes.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Indirect Routes to Aminoacyl-tRNA: The Diversity of Prokaryotic Cysteine Encoding Systems

Universally present aminoacyl-tRNA synthetases (aaRSs) stringently recognize their cognate tRNAs and acylate them with one of the proteinogenic amino acids. However, some organisms possess aaRSs that deviate from the accurate translation of the genetic code and exhibit relaxed specificity toward their tRNA and/or amino acid substrates. Typically, these aaRSs are part of an indirect pathway in which multiple enzymes participate in the formation of the correct aminoacyl-tRNA product. The indirect cysteine (Cys)-tRNA pathway, originally thought to be restricted to methanogenic archaea, uses the unique O -phosphoseryl-tRNA synthetase (SepRS), which acylates the non-proteinogenic amino acid O -phosphoserine (Sep) onto tRNA Cys . Together with Sep-tRNA:Cys-tRNA synthase (SepCysS) and the adapter protein SepCysE, SepRS forms a transsulfursome complex responsible for shuttling Sep-tRNA Cys to SepCysS for conversion of the tRNA-bound Sep to Cys. Here, we report a comprehensive bioinformatic analysis of the diversity of indirect Cys encoding systems. These systems are present in more diverse groups of bacteria and archaea than previously known. Given the occurrence and distribution of some genes consistently flanking SepRS, it is likely that this gene was part of an ancient operon that suffered a gradual loss of its original components. Newly identified bacterial SepRS sequences strengthen the suggestion that this lineage of enzymes may not rely on the m 1 G37 identity determinant in tRNA. Some bacterial SepRSs possess an N-terminal fusion resembling a threonyl-tRNA synthetase editing domain, which interestingly is frequently observed in the vicinity of archaeal SepCysS genes. We also found several highly degenerate SepRS genes that likely have altered amino acid specificity. Cross-analysis of selenocysteine (Sec)-utilizing traits confirmed the co-occurrence of SepCysE and the Sec-utilizing machinery in archaea, but also identified an unusual O -phosphoseryl-tRNA Sec kinase fusion with an archaeal Sec elongation factor in some lineages, where it may serve in place of SepCysE to prevent crosstalk between the two minor aminoacylation systems. These results shed new light on the variations in SepRS and SepCysS enzymes that may reflect adaptation to lifestyle and habitat, and provide new information on the evolution of the genetic code.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2016

Modification of the electronic properties of hexagonal boron-nitride in BN/graphene vertical heterostructures

Van der Waals (vdW) heterostructures consist of isolated atomic planar structures, assembled layer- by-layer into desired structures in a well-defined sequence. Graphene deposited on hexagonal boron nitride (h-BN) has been first considered as a testbed system for vdW heterostructures, and many others have been demonstrated both theoretically and experimentally, revealing many attractive properties and phenomena. However, much less emphasis has been placed on how graphene actively affects h-BN properties. Here, we perform local probe measurements on single-layer h-BN grown over graphene and highlight the manifestation of a proximity effect that significantly affects the electronic properties of h-BN due to its coupling with the underlying graphene. We find electronic states originating from the graphene layer and the Cu substrate to be injected into the wide electronic gap of the h-BN top layer. Such proximity effect is further confirmed in a study of the variation of h-BN in-gap states with interlayer couplings, elucidated using a combination of topographical/ spectroscopic measurements and first-principles density functional theory calculations. In conclusion, the findings of this work indicate the potential of mutually engineering electronic properties of the components of vdW heterostructures.

36 MATERIALS SCIENCE↗

DOE OSTI2020

Amplicon Sequencing Assessment to Measure Microbial Community Response from Heavy Metal Contaminated Soils in Savannah River site, Tims Branch watershed

Long-term presence of heavy metal contamination in soils is correlated with changes in microbial community structures and can lead to the reduction of indigenous species. Tolerance to soil heavy metal toxicity varies among different microbial communities. It remains unclear whether long term effects caused by metal perturbations are associated with soil microbial community dysregulation in Tims Branch watershed. Research Questions: Does heavy metal contamination in Tims Branch soils decrease microbial species diversity significantly compared to non-contaminated soils? Does the species richness decrease in contaminated samples? between samples? Is diversity in low, mid and high contamination soils significantly different compared to control samples? Hypothesis: The relative abundance and diversity of species are significantly altered in soils which are contaminated with heavy metals in the Tims Branch watershed. Objectives: Use amplicon sequencing technology along with bioinformatics to compare bacterial communities between four sites in Tims Branch watershed. Evaluate the percent relative abundance and diversity across the site. Conclusions: All bacterial communities exposed to different heavy metal concentrations were dominated by four major groups (Proteobacteria, Acidobacteria, Chloroflexi and Verrucomicrobia). The dominant phyla Proteobacteria, Acidobacteria and Chloroflexi accounted for 61 % of the relative abundance. Soil bacterial α-diversity, expressed as observed species richness metric and Shannon's diversity index, was the highest in sample location four (high contamination). The lowest observed was at the control location. Species richness increased when concentrations of heavy metals were localized, inferring that the metals can alter microbial community structure. PERMANOVA analysis between contaminated groups tested significant using the Adonis (F (4,29) =1.9712; p < 0.001). There is a 20 percent variation explained between groups (R2 = 0.214). This analysis proves to be beneficial in detecting microbial communities altered by contaminated soils. Moreover, certain concentrations of heavy metals can alter microbial community structures.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2020

Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum

Sugarcane is the most important sugar and biofuel crop. MADS-box genes encode transcription factors that are involved in developmental control and signal transduction in plants. Systematic analyses of MADS-box genes have been reported in many plant species, but its identification and characterization were not possible until a reference genome of autotetraploid wild type sugarcane specie, Saccharum spontaneum is available recently. We identified 182 MADS-box sequences in the S. spontaneum genome, which were annotated into 63 genes, including 6 (9.5%) genes with four alleles, 21 (33.3%) with three, 29 (46%) with two, 7 (11.1%) with one allele. Paralogs (tandem duplication and disperse duplicated) were also identified and characterized. These MADS-box genes were divided into two groups; Type-I (21 Mα, 4 Mβ, 4 Mγ) and Type-II (32 MIKCc, 2 MIKC*) through phylogenetic analysis with orthologs in Arabidopsis and sorghum. Structural diversity and distribution of motifs were studied in detail. Chromosomal localizations revealed that S. spontaneum MADS-box genes were randomly distributed across eight homologous chromosome groups. The expression profiles of these MADS-box genes were analyzed in leaves, roots, stem sections and after hormones treatment. Important alleles based on promoter analysis and expression variations were dissected. qRT-PCR analysis was performed to verify the expression pattern of pivotal S. spontaneum MADS-box genes and suggested that flower timing genes ( SOC1 and SVP ) may regulate vegetative development.

59 BASIC BIOLOGICAL SCIENCES↗