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At least 1,207 records · Page 67

Nutri‐cereal tissue‐specific transcriptome atlas during development: Functional integration of gene expression to identify mineral uptake pathways in little millet ( Panicum sumatrense )

SUMMARY Little millet (Panicum sumatrenseRoth ex Roem. & Schult.) is an essential minor millet of southeast Asia and Africa's temperate and subtropical regions. The plant is stress‐tolerant, has a short life cycle, and has a mineral‐rich nutritional profile associated with unique health benefits. We report the developmental gene expression atlas of little millet (genotype JK‐8) from ten tissues representing different stages of its life cycle, starting from seed germination and vegetative growth to panicle maturation. The developmental transcriptome atlas led to the identification of 342 827 transcripts. The BUSCO analysis and comparison with the transcriptomes of related species confirm that this study presents high‐quality, in‐depth coverage of the little millet transcriptome. In addition, the eFP browser generated here has a user‐friendly interface, allowing interactive visualizations of tissue‐specific gene expression. Using these data, we identified transcripts, the orthologs of which inArabidopsisand rice are involved in nutrient acquisition, transport, and response pathways. The comparative analysis of the expression levels of these transcripts holds great potential for enhancing the mineral content in crops, particularly zinc and iron, to address the issue of “hidden hunger” and to attain nutritional security, making it a valuable asset for translational research.

Plant Sciences↗

WE-D-9A-03: CSDF: A Color Extension of the Grayscale Standard Display Function

Purpose: Use of color images in medical imaging has increased significantly the last few years. As of today there is no agreed standard on how color information needs to be visualized on medical color displays, resulting into large variability of color appearance and it making consistency and quality assurance a challenge. This paper presents a proposal for an extension of DICOM GSDF towards color. Methods: Visualization needs for several color modalities (multimodality imaging, nuclear medicine, digital pathology, quantitative imaging applications…) have been studied. On this basis a proposal was made for desired color behavior of color medical display systems and its behavior and effect on color medical images was analyzed. Results: Several medical color modalities could benefit from perceptually linear color visualization for similar reasons as why GSDF was put in place for greyscale medical images. An extension of the GSDF (Greyscale Standard Display Function) to color is proposed: CSDF (color standard display function). CSDF is based on deltaE2000 and offers a perceptually linear color behavior. CSDF uses GSDF as its neutral grey behavior. A comparison between sRGB/GSDF and CSDF confirms that CSDF significantly improves perceptual color linearity. Furthermore, results also indicate that because of the improved perceptual linearity, CSDF has the potential to increase perceived contrast of clinically relevant color features. Conclusion: There is a need for an extension of GSDF towards color visualization in order to guarantee consistency and quality. A first proposal (CSDF) for such extension has been made. Behavior of a CSDF calibrated display has been characterized and compared with sRGB/GSDF behavior. First results indicate that CSDF could have a positive influence on perceived contrast of clinically relevant color features and could offer benefits for quantitative imaging applications. Authors are employees of Barco Healthcare.

60 APPLIED LIFE SCIENCES↗

SU-F-J-23: Field-Of-View Expansion in Cone-Beam CT Reconstruction by Use of Prior Information

Purpose: Cone-beam CT (CBCT) has become an integral part of online patient setup in an image-guided radiation therapy (IGRT). In addition, the utility of CBCT for dose calculation has actively been investigated. However, the limited size of field-of-view (FOV) and resulted CBCT image with a lack of peripheral area of patient body prevents the reliability of dose calculation. In this study, we aim to develop an FOV expanded CBCT in IGRT system to allow the dose calculation. Methods: Three lung cancer patients were selected in this study. We collected the cone-beam projection images in the CBCT-based IGRT system (X-ray volume imaging unit, ELEKTA), where FOV size of the provided CBCT with these projections was 410 × 410 mm{sup 2} (normal FOV). Using these projections, CBCT with a size of 728 × 728 mm{sup 2} was reconstructed by a posteriori estimation algorithm including a prior image constrained compressed sensing (PICCS). The treatment planning CT was used as a prior image. To assess the effectiveness of FOV expansion, a dose calculation was performed on the expanded CBCT image with region-of-interest (ROI) density mapping method, and it was compared with that of treatment planning CT as well as that of CBCT reconstructed by filtered back projection (FBP) algorithm. Results: A posteriori estimation algorithm with PICCS clearly visualized an area outside normal FOV, whereas the FBP algorithm yielded severe streak artifacts outside normal FOV due to under-sampling. The dose calculation result using the expanded CBCT agreed with that using treatment planning CT very well; a maximum dose difference was 1.3% for gross tumor volumes. Conclusion: With a posteriori estimation algorithm, FOV in CBCT can be expanded. Dose comparison results suggested that the use of expanded CBCTs is acceptable for dose calculation in adaptive radiation therapy. This study has been supported by KAKENHI (15K08691).

60 APPLIED LIFE SCIENCES↗

A Safe Response to Renewable Energy Hazards

The International Association of Fire Fighters (IAFF) and Underwriters Laboratories, LLC (UL) in conjunction with UL Solutions initiated a joint project in 2022 under an agreement with the United States Department of Energy-Office of Energy Efficiency and Renewable Energy (DOE-EERE). This project focused on two separate and important initiatives related to energy efficiency in residential buildings. Initiative 1: Fire Performance on Energy Efficient Exterior Walls. Initiative 2: Firefighting Tactics in Residential Properties with Building Energy Storage Systems (BESS). The project’s first initiative addresses concerns surrounding new technologies with enhanced, energy-efficient exterior walls installed on residential properties. The concerns of fire rapidly traveling vertically up the exterior of these walls were examined. This addressed a growing concern from the first responder community that many times, the fires on the exterior of residential buildings have already evolved into an attic fire by the time of arrival – making it problematic to address the fire scenario. The test plan for Initiative 1 incorporated a modified version of an American Society for Testing and Materials (ASTM) test method, ASTM E2707, Standard Test Method for Determining Fire Penetration of Exterior Wall Assemblies Using a Direct Flame Impingement Exposure, as the foundation of the research. The test method involved a wall structure intended to represent retrofit construction to evaluate how fire would spread vertically or laterally. The second aspect of the UL-IAFF Project focuses on the fire service response to Residential Battery Energy Storage System (RBESS) incidents. These simulation tests were constructed in the large-scale fire test facility at UL Solutions’ Northbrook, IL campus. A baseline test was conducted that involved a test structure with no batteries—shelving units populated with standardized commodities, representing a typical garage with cellulosic and plastic contents. Three additional tests have been conducted to generate data with the contribution of energy storage system (ESS) batteries to compare fire and explosion hazards against the baseline test. Through this work, fire service tactical considerations can be explored. From the data, the team can determine 1) the visual indicators of a residential fire that has involved an RBESS (or, potentially, other large batteries) and 2) the impact of fire service-initiated ventilation of the structure on the fire conditions and explosion risks.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Automated electroencephalography system and electroencephalographic correlates of space motion sickness, part 3

Computer quantification methods were used to analyze the Skylab electroencephalographic data obtained during the course of the M133 series of experiments. This undertaking was prompted by initial observations made during visual analysis of the tape-recorded sleep records where there appeared to be an increase of the alpha-rhythm frequency during some inflight recording sessions, as compared to preflight baseline observations. A number of potential etiological factors are identified and their various possible influences discussed. The presence of the zero-g state is thought to be an important factor, possibly influencing EEG through alteration of vestibular function and/or by producing fluid shifts secondary to loss of hydrostatic pressure.

Frost, J. D., Jr.↗

Solar maximum thermal surface assessment

The inflight repair of the Solar Maximum Spacecraft provided the first opportunity to make actual measurements of thermal control surfaces after 4 years exposure in low Earth orbit. Defective hardware was replaced by astronauts and returned to Earth while protected from reentry damage in the Shuttle Payload bay. A preliminary thermal surface assessment was made soon after retrieval in support of Space Telescope and other current spacecraft programs. This included visual examination and measurement of Kapton and Teflon film to determine change in thermal radiative properties after 4 years exposure to solar radiation and reaction with atomic oxygen. Comparative measurements were made with a portable solar reflectometer used for inspection of spacecraft hardware. Post flight measurements and observations reveal significant surface changes that further confirm Kapton mass loss predictions made prior to Solar Maximum repair. Details of thermal surface application, measurements and experimental results are presented and discussed.

Rhoads, G. D.↗

Star formation in the Magellanic irregular galaxy NGC 4449

New NIR and FIR maps and J = 1-0 CO spectroscopy of the Magellanic irregular galaxy NGC 4449 are presented. The brighter 150-micron emission is concentrated along the central visual ridge of the galaxy, although there is lower intensity extended emission throughout the visible extent of the object. The maximum FIR emission is coincident, within the uncertainties, with the visual and NIR maxima, identified as the galactic nucleus. It is estimated that the IR luminosity of the 1-kpc-diameter central region in NGC 4449 is comparable to that for a similar-sized region at the center of the Galaxy. A large fraction of the 150-micron emission may arise from warm dust distributed throughout the galaxy and heated by the diffuse radiation field. Active star formation follows the NIR emission in part of the galaxy, but no coincidence is found in another region.

Thronson, Harley A., Jr.↗

Studies of vortex flow aerodynamics using CFD flow visualizations

Navier-Stokes computations of three-dimensional vortical flows over a round-edged double-delta wing and a tangent-ogive forebody are performed using an implicit upwind finite-difference scheme. Computed particle traces are compared with experimental oil-flow streaks.

Hsu, C.-H.↗

Responsivity of silicon photodiodes from 0.5 to 1.1 micron at 77 K

The responsivities of Si, Ge, and InSb photodiodes at 77 K are measured and compared. The results are used to determine the wavelength at which a change should occur in the detector materials used in the focal plan of the Mars Observer Visual and IR Mapping Spectrometer (VIMS). Quantitative responsivity data for Si photodiodes in the band from 0.5 to 1.1 were needed for the VIMS system analysis. The results show that the Si array for the focal plane should have two different antireflection coatings. It is found that the Si and InSb materials have equivalent quantum efficiencies at about 0.9 microns. Because of unknown signal chain complications that could be caused by a multiplexer accessing two different capacitances, the focal plane was designed with a change from Si to InSb at 1 micron.

Staller, C.↗

Lightcurve of comet Austin(1989c1) and its dust mantle development

Brightness variations of comet Austin(1989c1) were investigated in terms of the variations of water production rate. We translated the visual brightness data into water production rates using Newburn's semi-empirical law. The curve of the water production rates as a function of heliocentric distance was compared with the model calculations that assumed energy balance between the solar incident and vaporization of water. Thermal flow in a dust mantle at a surface of the nucleus is also included in the model. The model calculations including the dust mantle are more favorable for the observed rate than non-dust mantle cases. The extinction after the perihelion passage suggests that the dust mantle developed gradually.

Hasegawa, Hitoshi↗

Dynamic stall study of a multi-element airfoil

Unsteady flow behavior and load characteristics of a VR-7 airfoil with and without a slat were studied in the water tunnel of the Aeroflightdynamics Directorate, NASA Ames Research Center. Both airfoils were oscillated sinusoidally between 5 and 25 degrees at a Reynolds number of 200,000 to obtain the unsteady lift, drag and pitching moment data. A fluorescing dye was released from an orifice located at the leading edge of the airfoil for the purpose of visualizing the boundary layer and wake flow. The flow field and load predictions of an incompressible Navier-Stokes code based on a velocity-vorticity formulation were compared with the test data. The test and predictions both confirm that the slatted VR-7 airfoil delays both static and dynamic stall as compared to the VR-7 airfoil alone.

Tung, Chee↗

Reduction of the elevator illusion from continued hypergravity exposure and visual error-corrective feedback

Ten subjects served as their own controls in two conditions of continuous, centrifugally produced hypergravity (+2 Gz) and a 1-G control condition. Before and after exposure, open-loop measures were obtained of (1) motor control, (2) visual localization, and (3) hand-eye coordination. During exposure in the visual feedback/hypergravity condition, subjects received terminal visual error-corrective feedback from their target pointing, and in the no-visual feedback/hypergravity condition they pointed open loop. As expected, the motor control measures for both experimental conditions revealed very short lived underreaching (the muscle-loading effect) at the outset of hypergravity and an equally transient negative aftereffect on returning to 1 G. The substantial (approximately 17 degrees) initial elevator illusion experienced in both hypergravity conditions declined over the course of the exposure period, whether or not visual feedback was provided. This effect was tentatively attributed to habituation of the otoliths. Visual feedback produced a smaller additional decrement and a postexposure negative after-effect, possible evidence for visual recalibration. Surprisingly, the target-pointing error made during hypergravity in the no-visual-feedback condition was substantially less than that predicted by subjects' elevator illusion. This finding calls into question the neural outflow model as a complete explanation of this illusion.

NASA Program Space Physiology and Countermeasures↗

Background Oriented Schlieren Applied to Study Shock Spacing in a Screeching Circular Jet

Background oriented schlieren (BOS) is a recent development of the schlieren and shadowgraph methods. The BOS technique has the ability to provide visualizations of the density gradient in both the axial and radial directions. The resultant magnitude of the density gradients allows for comparison with shadowgraph images. This paper first compares data obtained by the BOS and shadowgraph techniques at identical conditions in a free jet. The patterns and spacing of the shock trains obtained by the two techniques are found to be consistent with one another. This provides confidence in the shock spacing measurement by the BOS technique. Due to its simpler setup, BOS is then applied to investigate the shock spacing associated with the screech phenomenon, especially during stage jumps. Screech frequencies from a 37.6 mm convergent nozzle, as a function of jet Mach number (M(sub j)), are shown to exhibit various stages. As many as eight stages are identified with the present nozzle over the range 1.0 < M(sub j) <1.7. BOS images are acquired at various screech conditions and the shock spacing is examined as a function of M(sub j).

Clem, Michelle M.↗

Mechanical Stress and Antioxidant Protection in the Retina of Hindlimb Suspended Rats

It has been postulated that hindlimb suspension (HS) causes a cephalad fluid shift in quadrupeds similar to that occurring to humans in microgravity. Therefore, HS may provide a suitable animal model in which to recapitulate the ocular changes observed in the human Visual Impairment and Intracranial Pressure (VIIP) syndrome. This work reports preliminary results from a tissue sharing project using 34 week-old Brown Norway rats. Two different experiments compared normal posture controls and HS rats for 2 weeks and rats exposed to HS for 2 weeks but allowed to recover in normal posture for 2 additional weeks. The effects of two nutritional countermeasures, green tea extract (GT) and plant polyphenol resveratrol (Rv), were also evaluated. Green tea contains the antioxidant epigallocatechin gallate (EGCG). qPCR gene expression analysis of selected targets was performed on RNA from isolated retinas, and histologic analysis was done on one fixed eye per rat. The transcription factor early growth response protein 1 (Egr1) was upregulated almost 2-fold in HS retinas relative to controls (P = 0.059), and its expression returned to control levels after 2 weeks of recovery in normal posture (P = 0.023). HS-induced upregulation of Egr1 was attenuated (but not significantly) in retinas from rats fed an antioxidant rich (GT extract) diet. In rats fed the GT-enriched diet, antioxidant enzymes were induced, evidenced by the upregulation of the gene heme oxygenase 1 (Hmox1) (P = 0.042) and the gene superoxide dismutase 2 (Sod2) (P = 0.0001). Egr1 is a stretch-activated transcription factor, and the Egr1 mechanosensitive response to HS may have been caused by a change in the translaminal pressure and/or mechanical deformation of the eye globe. The observed histologic measurements of the various retinal layers in the HS rats were lower in value than those of the control animal (n = 1), however insufficient data were available for statistical analysis. Aquaporin 4, a water-selective channel involved in interstitial fluid homeostasis, showed an upregulated trend in HS retinas; however, these results are preliminary. Total retinal thickness increased significantly (P = 0.049) in HS rats fed a resveratrol enriched diet compared to HS rats on a normal diet. This change appeared to be reversed during the 2 weeks of recovery post HS, but no differences in retina thickness were observed between HS animals and HS recovered animals when both groups consumed a normal diet. The reversibility of the increase in retinal thickness induced by resveratrol during HS may therefore reflect an interaction between the stress provoked by HS and the cytoprotective mechanisms elicited by resveratrol

Glass, Aziza↗

Air Traffic Management TestBed: Messaging Performance

The Air Traffic Management (ATM) TestBed is an air traffic management modeling and simulation platform and framework developed by the National Aeronautics and Space Administration (NASA) to help design, configure, integrate, run, and monitor air traffic simulations. The communication middleware, implemented in the TestBed framework layer, is a core feature for data message exchange. The feature provides an abstraction layer called Messaging Support to allow switching one middleware to another without a need to rebuild the simulation components. Messaging performance such as latencies, run durations, and throughputs are important factors. Low latencies can produce accurate results in high-fidelity and visualization models. Short run durations are preferred because better run efficiency can be achieved. High throughputs allow more runs to be executed concurrently. This technical memorandum studies and compares the messaging performance by running a full-day, fast-time simulation using three communication middleware as well as tweaking the default communication middleware settings used by the TestBed. Results indicate that the messaging performance could be improved by disabling either compression or persistence settings, while the run duration and throughput could be further improved by disabling both settings with a tradeoff of the message latencies increased by a factor of ten.

Chok Fung Lai↗

Blunt body near wake flow field at Mach 6

Tests were conducted in a Mach 6 flow to examine the reattachment process of an axisymmetric free shear layer associated with the near wake of a 70 deg. half angle, spherically blunted cone with a cylindrical after body. Model angle of incidence was fixed at 0 deg. and free-stream Reynolds numbers based on body diameter ranged from 0.5 x 10(exp 6) to 4 x 10(exp 6). The sensitivity of wake shear layer transition on reattachment heating was investigated. The present perfect gas study was designed to compliment results obtained previously in facilities capable of producing real gas effects. The instrumented blunted cone model was designed primarily for testing in high enthalpy hypervelocity shock tunnels in both this country and abroad but was amenable for testing in conventional hypersonic blowdown wind tunnels as well. Surface heating rates were inferred from temperature - time histories from coaxial surface thermocouples on the model forebody and thin film resistance gages along the model base and cylindrical after body. General flow feature (bow shock, wake shear layer, and recompression shock) locations were visually identified by schlieren photography. Mean shear layer position and growth were determined from intrusive pitot pressure surveys. In addition, wake surveys with a constant temperature hot-wire anemometer were utilized to qualitatively characterize the state of the shear layer prior to reattachment. Experimental results were compared to laminar perfect gas predictions provided by a 3-D Navier Stokes code (NSHYP). Shear layer impingement on the instrumented cylindrical after body resulted in a localized heating maximum that was 21 to 29 percent of the forebody stagnation point heating. Peak heating resulting from the reattaching shear layer was found to be a factor of 2 higher than laminar predictions, which suggested a transitional shear layer. Schlieren flow visualization and fluctuating voltage time histories and spectra from the hot wire surveys across the shear layer substantiate this observation. The sensitivity of surface heating to forebody roughness was characterized for a reattaching shear layer. For example, at R(sub infinity), d = 4 x 10(exp 6), when the shear layer was transitional, the magnitude of peak heating from shear layer impingement was reduced by approximately 24 percent when transition grit was applied to the forebody. The spatial location of the local peak, however, remained unchanged.

Horvath, Thomas J.↗

How does C-VIEW image quality compare with conventional 2D FFDM?

Purpose: The FDA approved the use of digital breast tomosynthesis (DBT) in 2011 as an adjunct to 2D full field digital mammography (FFDM) with the constraint that all DBT acquisitions must be paired with a 2D image to assure adequate interpretative information is provided. Recently manufacturers have developed methods to provide a synthesized 2D image generated from the DBT data with the hope of sparing patients the radiation exposure from the FFDM acquisition. While this much needed alternative effectively reduces the total radiation burden, differences in image quality must also be considered. The goal of this study was to compare the intrinsic image quality of synthesized 2D C-VIEW and 2D FFDM images in terms of resolution, contrast, and noise. Methods: Two phantoms were utilized in this study: the American College of Radiology mammography accreditation phantom (ACR phantom) and a novel 3D printed anthropomorphic breast phantom. Both phantoms were imaged using a Hologic Selenia Dimensions 3D system. Analysis of the ACR phantom includes both visual inspection and objective automated analysis using in-house software. Analysis of the 3D anthropomorphic phantom includes visual assessment of resolution and Fourier analysis of the noise. Results: Using ACR-defined scoring criteria for the ACR phantom, the FFDM images scored statistically higher than C-VIEW according to both the average observer and automated scores. In addition, between 50% and 70% of C-VIEW images failed to meet the nominal minimum ACR accreditation requirements—primarily due to fiber breaks. Software analysis demonstrated that C-VIEW provided enhanced visualization of medium and large microcalcification objects; however, the benefits diminished for smaller high contrast objects and all low contrast objects. Visual analysis of the anthropomorphic phantom showed a measureable loss of resolution in the C-VIEW image (11 lp/mm FFDM, 5 lp/mm C-VIEW) and loss in detection of small microcalcification objects. Spectral analysis of the anthropomorphic phantom showed higher total noise magnitude in the FFDM image compared with C-VIEW. Whereas the FFDM image contained approximately white noise texture, the C-VIEW image exhibited marked noise reduction at midfrequency and high frequency with far less noise suppression at low frequencies resulting in a mottled noise appearance. Conclusions: Their analysis demonstrates many instances where the C-VIEW image quality differs from FFDM. Compared to FFDM, C-VIEW offers a better depiction of objects of certain size and contrast, but provides poorer overall resolution and noise properties. Based on these findings, the utilization of C-VIEW images in the clinical setting requires careful consideration, especially if considering the discontinuation of FFDM imaging. Not explicitly explored in this study is how the combination of DBT + C-VIEW performs relative to DBT + FFDM or FFDM alone.

60 APPLIED LIFE SCIENCES↗

Visualization of Iron‐Binding Micelles in Acidic Recombinant Biomineralization Protein, MamC

Biological macromolecules are utilized in low‐temperature synthetic methods to exert precise control over nanoparticle nucleation and placement. They enable low‐temperature formation of a variety of functional nanostructured materials with properties often not achieved via conventional synthetic techniques. Here we report on the in situ visualization of a novel acidic bacterial recombinant protein, MamC, commonly present in the magnetosome membrane of several magnetotactic bacteria, including Magnetococcus marinus , strain MC‐1. Our findings provide an insight into the self‐assembly of MamC and point to formation of the extended protein surface, which is assumed to play an important role in the formation of biotemplated inorganic nanoparticles. The self‐organization of MamC is compared to the behavior of another acidic recombinant iron‐binding protein, Mms6.

Kashyap, Sanjay↗