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At least 1,243 records · Page 69

Desensitization and recovery of phototropic responsiveness in Arabidopsis thaliana

Phototropism is induced by blue light, which also induces desensitization, a partial or total loss of phototropic responsiveness. The fluence and fluence-rate dependence of desensitization and recovery from desensitization have been measured for etiolated and red light (669-nm) preirradiated Arabidopsis thaliana seedlings. The extent of desensitization increased as the fluence of the desensitizing 450-nm light was increased from 0.3 to 60 micromoles m-2 s-1. At equal fluences, blue light caused more desensitization when given at a fluence rate of 1.0 micromole m-2 s-1 than at 0.3 micromole m-2 s-1. In addition, seedlings irradiated with blue light at the higher fluence rate required a longer recovery time than seedlings irradiated at the lower fluence rate. A red light preirradiation, probably mediated via phytochrome, decreased the time required for recovery from desensitization. The minimum time for detectable recovery was about 65 s, and the maximum time observed was about 10 min. It is proposed that the descending arm of the fluence-response relationship for first positive phototropism is a consequence of desensitization, and that the time threshold for second positive phototropism establishes a period during which recovery from desensitization occurs.

NASA Program Space Biology↗

Evaluation of radiation effects against C6 glioma in combination with vaccinia virus-p53 gene therapy

The primary objective of this study was to evaluate the antitumor effects of recombinant vaccinia virus-p53 (rVV-p53) in combination with radiation therapy against the C6 rat glioma, a p53 deficient tumor that is relatively radioresistant. VV-LIVP, the parental virus (Lister strain), was used as a control. Localized treatment of subcutaneous C6 tumors in athymic mice with either rVV-p53 or VV-LIVP together with tumor irradiation resulted in low tumor incidence and significantly slower tumor progression compared to the agents given as single modalities. Assays of blood and spleen indicated that immune system activation may account, at least partly, for the enhance tumor inhibition seen with combined treatment. No overt signs of treatment-related toxicity were noted.

NASA Discipline Radiation Health↗

Calcium influences sensitivity to growth inhibition induced by a cell surface sialoglycopeptide

While studies concerning mitogenic factors have been an important area of research for many years, much less is understood about the mechanisms of action of cell surface growth inhibitors. We have purified an 18 kDa cell surface sialoglycopeptide growth inhibitor (CeReS-18) which can reversibly inhibit the proliferation of diverse cell types. The studies discussed in this article show that three mouse keratinocyte cell lines exhibit sixty-fold greater sensitivity than other fibroblasts and epithelial-like cells to CeReS-18-induced growth inhibition. Growth inhibition induced by CeReS-18 treatment is a reversible process, and the three mouse keratinocyte cell lines exhibited either single or multiple cell cycle arrest points, although a predominantly G0/G1 cell cycle arrest point was exhibited in Swiss 3T3 fibroblasts. The sensitivity of the mouse keratinocyte cell lines to CeReS-18-induced growth inhibition was not affected by the degree of tumorigenic progression in the cell lines and was not due to differences in CeReS-18 binding affinity or number of cell surface receptors per cell. However, the sensitivity of both murine fibroblasts and keratinocytes could be altered by changing the extracellular calcium concentration, such that increased extracellular calcium concentrations resulted in decreased sensitivity to CeReS-18-induced proliferation inhibition. Thus the increased sensitivity of the murine keratinocyte cell lines to CeReS-18 could be ascribed to the low calcium concentration used in their propagation. Studies are currently under way investigating the role of calcium in CeReS-18-induced growth arrest. The CeReS-18 may serve as a very useful tool to study negative growth control and the signal transduction events associated with cell cycling.

NASA Discipline Cell Biology↗

Effective conductivity of wire mesh reflectors for space deployable antenna systems

This report summarizes efforts to characterize the measurement of conductive mesh and smooth surfaces using proximity measurements for a dielectric resonator. The resonator operates in the HEM11 mode and is shown to have an evanescent field behavior in the vicinity of the sample surface, raising some question to the validity of measurements requiring near normal incidence on the material. In addition, the slow radial field decay outside of the dielectric resonator validates the sensitivity to the planar supporting structure and potential radiation effects. Though these concerns become apparent along with the sensitivity to the gap between the dielectric and the material surface, the basic concept of the material measurement using dielectric resonators has been verified for useful comparison of material surface properties. The properties, particularly loss, may be obtained by monitoring the resonant frequency along with the resonator quality factor (Q), 3 dB bandwidth, or the midband transmission amplitude. Comparison must be made to known materials to extract the desired data.

Davis, William A.↗

The Type II superluminous SN 2008es at late times: near-infrared excess and circumstellar interaction

SN 2008es is one of the rare cases of a Type II superluminous supernova (SLSN), showing no narrow features in its early-time spectra, and therefore, its powering mechanism is under debate between circumstellar interaction (CSI) and magnetar spin-down. Late-time data are required for better constraints. We present optical and near-infrared (NIR) photometry obtained from Gemini, Keck, and Palomar Observatories from 192 to 554 d after explosion. Only broad H α emission is detected in a Gemini spectrum at 288 d. The line profile exhibits red-wing attenuation relative to the early-time spectrum. In addition to the cooling SN photosphere, an NIR excess with blackbody temperature ∼1500 K and radius ∼1016 cm is observed. This evidence supports dust condensation in the cool dense shell being responsible for the spectral evolution and NIR excess. We favour CSI, with ∼2–3 Mꙩ of circumstellar material (CSM) and ∼10–20 Mꙩ of ejecta, as the powering mechanism, which still dominates at our late-time epochs. Both models of uniform density and steady wind fit the data equally well, with an effective CSM radius ∼1015 cm, supporting the efficient conversion of shock energy to radiation by CSI. A low amount (≲0.4 Mꙩ) of 56Ni is possible but cannot be verified yet, since the light curve is dominated by CSI. The magnetar spin-down powering mechanism cannot be ruled out, but is less favoured because it overpredicts the late-time fluxes and may be inconsistent with the presence of dust.

Kornpob Bhirombhakdi↗

Isoprene: New insights into the control of emission and mediation of stress tolerance by gene expression

Isoprene is a volatile compound produced in large amounts by some, but not all, plants by the enzyme isoprene synthase. Plants emit vast quantities of isoprene, with a net global output of 600 Tg per year, and typical emission rates from individual plants around 2% of net carbon assimilation. There is significant debate about whether global climate change resulting from increasing CO 2 in the atmosphere will increase or decrease global isoprene emission in the future. We show evidence supporting predictions of increased isoprene emission in the future, but the effects could vary depending on the environment under consideration. For many years, isoprene was believed to have immediate, physical effects on plants such as changing membrane properties or quenching reactive oxygen species. Although observations sometimes supported these hypotheses, the effects were not always observed, and the reasons for the variability were not apparent. Although there may be some physical effects, recent studies show that isoprene has significant effects on gene expression, the proteome, and the metabolome of both emitting and nonemitting species. Consistent results are seen across species and specific treatment protocols. Finally, this review summarizes recent findings on the role and control of isoprene emission from plants.

59 BASIC BIOLOGICAL SCIENCES↗

Isotopic and Fuel Lattice Parameter Trends in Extended Enrichment and Higher Burnup LWR Fuel Vol I: PWR fuel

Commercial light water reactor (LWR) operators and fuel vendors in the United States are pursuing changes to nuclear fuel that include extended enrichment (EE) and accident-tolerant fuel (ATF) designs. The term EE (8% > 235 U > 5%) is used in this report to refer to a subset of high assay low-enriched uranium (HALEU) that is considered usable in commercial US LWRs in the near term. ATF features are designed to improve fuel system performance under accident conditions. One goal of EE is to improve fuel cycle economy by enabling fuel to be depleted to higher burnup than the typical current maximum pin burnup limits (62 gigawatt-days per metric ton of uranium [GWd/MTU]). Adoption of EE, ATF, and high burnup (HBU) fuels in the US commercial fleet requires a clear understanding of the effects on core physics parameters and used fuel isotopic content, as well as confidence in the accuracy of computer code predictions over an expanded range of materials, enrichment, and burnup. A thorough understanding of the applicability and adequacy of benchmark data (e.g., criticality, decay heat, isotopic content) for computer code validation is necessary to ensure that appropriate safety margins are maintained. To prepare for and support these potential changes, the effects of EE, ATF, and HBU are being assessed for selected representative LWR fuel designs. The project is divided into phases: this report summarizes the findings of Phase 1, which focuses on the lattice physics parameter and used fuel isotopic changes for a conventional Westinghouse 17×17 pressurized water reactor (PWR) design. The primary investigation tool is the SCALE Polaris code using the SCALE 56-group Evaluated Nuclear Data File (ENDF)/B-VII.1 cross sections. The goal of the current work is to (1) identify and explain important effects of EE and HBU (reactivity, lattice physics, and isotopic effects) assuming that PWR fuel design and usage remain similar to those for current enrichment fuel, (2) provide limited code-to-code comparisons with higher order cross section libraries and/or codes, and (3) identify any apparent anomalous trends in the results for further investigation.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

A low, adaptive dose of gamma-rays reduced the number and altered the spectrum of S1- mutants in human-hamster hybrid AL cells

We examined the effects of a low, adaptive dose of 137Cs-gamma-irradiation (0.04 Gy) on the number and kinds of mutants induced in AL human-hamster hybrid cells by a later challenge dose of 4 Gy. The yield of S1- mutants was significantly less (by 53%) after exposure to both the adaptive and challenge doses compared to the challenge dose alone. The yield of hprt- mutants was similarly decreased. Incubation with cycloheximide (CX) or 3-aminobenzamide largely negated the decrease in mutant yield. The adaptive dose did not perturb the cell cycle, was not cytotoxic, and did not of itself increase the mutant yield above background. The adaptive dose did, however, alter the spectrum of S1- mutants from populations exposed only to the adaptive dose, as well as affecting the spectrum of S1- mutants generated by the challenge dose. The major change in both cases was a significant increase in the proportion of complex mutations compared to small mutations and simple deletions.

Non-NASA Center↗

Insights of biomass recalcitrance in natural Populus trichocarpa variants for biomass conversion

Populus has been investigated as a promising biomass feedstock for alternative fuels and chemicals. Physicochemical characteristics and genomic information of biomass feedstocks are among the essential information that can help not only advance our understanding of biomass recalcitrance but also in its efficient utilization. Here in this paper, the recalcitrance of natural Populus variants was elucidated in three aspects: (1) sugar release, (2) physicochemical properties, and (3) relative variation of gene expression within natural poplar variants. The sugar release performance of natural Populus variants was evaluated with their correlation with biomass recalcitrance-related characteristics. Among the physicochemical properties of poplar, the lignin content, lignin molecular weight, lignin S/G ratio, and cellulose accessibility were found to correlate with sugar release. The results demonstrated that the lignin content was negatively correlated with sugar release, whereas the lignin molecular weight, lignin S/G ratio, and cellulose accessibility were positively associated with poplar sugar release. The trend of differential gene expression of each variant also supports the characterization results and their effects on biomass conversion.

09 BIOMASS FUELS↗

Results of the Particulate Contamination Control Trade Study for Space Suit Life Support Development

As the United States plans to return astronauts to the moon and eventually to Mars, designing the most effective, efficient, and robust space suit life support system that will operate successfully in these dusty environments is vital. There is some knowledge of the contaminants and level of infiltration expected from the Lunar and Mars dust, however risk mitigation strategies and filtration designs to prevent contamination within the space suit life support system are still undefined. A trade study was initiated to identify and address these concerns, and to develop new requirements for the Constellation Space Suit Element (CSSE) Portable Life Support System (PLSS). This trade study investigates historical methods of particulate contamination control in space suits and vehicles, and evaluated the possibility of using commercial technologies for this application. In addition, the trade study examined potential filtration designs. This paper summarizes the results of this trade study.

Cognata, Thomas J.↗

Enhancing the Operational Resilience of Advanced Reactors with Digital Twins by Recurrent Neural Networks

Because of a lack of operation data during abnormal and accident scenarios, along with the existence of uncertainty in the evaluation model for transient and accident analysis, the established abnormal and emergency operating procedures can be biased in characterizing the reactor states and ensuring operational resilience. To improve state awareness and ensure operational flexibility for minimizing effects on the system due to anomaly, digital twin (DT) technology is suggested to support operator's decision-making by effectively extracting and using knowledge of the current and future plant states from the knowledge base. To demonstrate DT's capability for recovering the complete states of reactors and for predicting the future reactor behaviors, this paper develops and assesses both the diagnosis and prognosis DTs in a nearly autonomous management and control system for an Experimental Breeder Reactor-II simulator during different loss-of-flow scenarios.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Comparative study of the superconductivity in the Holstein and optical Su-Schrieffer-Heeger models

Theoretical studies suggest that Su-Schrieffer-Heeger-like electron-phonon (e-ph) interactions can mediate high-temperature bipolaronic superconductivity that is robust against repulsive electron-electron interactions. Here we present a comparative analysis of the pairing and competing charge/bond correlations in the two-dimensional Holstein and optical Su-Schrieffer-Heeger (SSH) models using numerically exact determinant quantum Monte Carlo. We find that the SSH interactions support light bipolarons and strong superconducting correlations out to relatively large values of the e-ph coupling λ and densities near half-filling, while the Holstein interaction does not due to the formation of heavy bipolarons and competing charge-density-wave order. We further find that the Holstein and SSH models have comparable pairing correlations in the weak coupling limit for carrier concentrations < n > <<1, where competing orders and polaronic effects are absent. These results support the proposal that SSH (bi)polarons can support superconductivity to larger values of λ in comparison to the Holstein polaron, but that the resulting T c gains are small in the weak coupling limit. We also find that the SSH model's pairing correlations are suppressed after including a weak on-site Hubbard repulsion. Furthermore, these results have important implications for identifying and engineering bipolaronic superconductivity.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Modeling of rolling element bearing mechanics. Theoretical manual

This report documents the theoretical basis for the Rolling Element Bearing Analysis System (REBANS) analysis code which determines the quasistatic response to external loads or displacement of three types of high-speed rolling element bearings: angular contact ball bearings; duplex angular contact ball bearings; and cylindrical roller bearings. The model includes the effects of bearing ring and support structure flexibility. It is comprised of two main programs: the Preprocessor for Bearing Analysis (PREBAN) which creates the input files for the main analysis program; and Flexibility Enhanced Rolling Element Bearing Analysis (FEREBA), the main analysis program. A companion report addresses the input instructions for and features of the computer codes. REBANS extends the capabilities of the SHABERTH (Shaft and Bearing Thermal Analysis) code to include race and housing flexibility, including such effects as dead band and preload springs.

Merchant, David H.↗

Role of Alumina Basicity in CO 2 Uptake in 3‐Aminopropylsilyl‐Grafted Alumina Adsorbents

Abstract Oxide‐supported amine materials are widely known to be effective CO 2 sorbents under simulated flue‐gas and direct‐air‐capture conditions. Most work has focused on amine species loaded onto porous silica supports, though potential stability advantages may be offered through the use of porous alumina supports. Unlike silica materials, which are comparably inert, porous alumina materials can be tuned to have substantial acidity and/or basicity. Owing to their amphoteric nature, alumina supports play a more active role in CO 2 sorption than silica supports, potentially directly participating in the adsorption process. In this work, primary amines associated with 3‐aminopropyltriethoxysilane are grafted onto two different mesoporous alumina materials having different levels of basicity. Adsorbent materials with different amine loadings are prepared, and the CO 2 ‐adsorption behavior of similar amines on the two alumina supports is demonstrated to be different. At low amine loadings, the inherent properties of the support surface play a significant role, whereas at high amine loadings, when the alumina surface is effectively blocked, the sorbents prepared on the two supports behave similarly. At high amine loadings, amine–CO 2 –amine interactions are shown to dominate, leading to adsorbed species that appear similar to the species formed over silica‐supported amine materials. The sorbent properties are comprehensively characterized using N 2 physisorption analysis, in situ FTIR spectroscopy, and adsorption microcalorimetry.

Potter, Matthew E.↗

Uniform Pt/Pd Bimetallic Nanocrystals Demonstrate Platinum Effect on Palladium Methane Combustion Activity and Stability

Bimetallic catalytic materials are in widespread use for numerous reactions, as the properties of a monometallic catalyst are often improved upon addition of a second metal. In studies with bimetallic catalysts, it remains challenging to establish clear structure–property relationships using traditional impregnation techniques, due to the presence of multiple coexisting active phases of different sizes, shapes, and compositions. Here, a convenient approach to prepare small and uniform Pt/Pd bimetallic nanocrystals with tailorable composition is demonstrated, despite the metals being immiscible in the bulk. By depositing this set of controlled nanocrystals onto a high-surface-area alumina support, we systematically investigate the effect of adding platinum to palladium catalysts for methane combustion. At low temperatures and in the absence of steam, all bimetallic catalysts show activity nearly identical with that of Pt/Al 2 O 3 , with much lower rates in comparison to that of the Pd/Al 2 O 3 sample. BUt, unlike Pd/Al 2 O 3 , which experiences severe low-temperature steam poisoning, all Pt/Pd bimetallic catalysts maintain combustion activity on exposure to excess steam. These features are due to the influence of Pt on the Pd oxidation state, which prevents the formation of a bulk-type PdO phase. Despite lower initial combustion rates, hydrothermal aging of the Pd-rich bimetallic catalyst induces segregation of a PdO phase in close contact to a Pd/Pt alloy phase, forming more active and highly stable sites for methane combustion. Altogether, this work unambiguously clarifies the activity and stability attributes of Pt/Pd phases which often coexist in traditionally synthesized bimetallic catalysts and demonstrates how well-controlled bimetallic catalysts elucidate structure–property relationships.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

An experimental-theoretical study of free vibrations of plates on elastic point supports

A theoretical and experimental study is made to investigate the effect on plate vibrations of varying the stiffness of corner elastic point supports. A theoretical model is developed using a Rayleigh-Ritz analysis which approximates the plate mode shapes as products of free-free beam modes. The elastic point supports are modelled both as massless translational springs, and springs with tip masses. The tip masses are included to better represent the experimental supports. An experiment is constructed using the bending stiffness of horizontal beams to support a square plate at its four corners. The stiffness of these supports can be varied over such a range that the plate fundamental frequency is lowered to 40% of the rigid support frequency. The variation with support stiffness of the frequencies of the first eight plate modes is measured, and compared with the theoretical results. The plate mode shapes for rigid supports are analyzed using holographic interferometry. There is excellent agreement between the theoretical and experimental results, except for high plate modes where the theoretical model is demonstrated to be inadequate.

Leuner, T. R.↗

An experimental-theoretical study of free vibrations of plates on elastic point supports

A theoretical and experimental study is made to investigate the effect on plate vibrations of varying the stiffness of corner elastic point supports. An experiment is conducted in which the bending stiffness of horizontal beams is used to support a square plate at its four corners. The stiffness of these supports can be varied over such a range that the plate fundamental frequency is lowered to 40% of the rigid support frequency. The variation with support stiffness of the frequencies of the first eight plate modes is measured and is compared with results from a theoretical model in which a Rayleigh-Ritz analysis is used which approximates the plate mode shapes as products of free-free beam modes. The elastic point supports are modeled both as massless translational springs and springs with tip masses, which are included to better represent the experimental supports.

Leuner, T. R.↗

Oncogenic transformation through the cell cycle and the LET dependent inverse dose rate effect

Synchronised populations of mouse C3H/10T-1/2 cells were obtained by a stringent mitotic dislodgment procedure. Mitotic cells rapidly attach and progress sequentially through the cell cycle. Irradiation (3 Gy of X rays) was carried out at intervals from 0 to 18 h after initiating cell cycle progression of the mitotic cells. Oncogenic transformation was enhanced 10-fold over cells irradiated soon after replating (G1 and S phases) for cells in a near 2 h period corresponding to cells in G2 phase but not in mitosis. The cell surviving fraction had a 2-1/2-fold variation with resistant peaks corresponding to the late G1 and late S phases. These findings provide experimental support for the hypothesis initiated by Rossi and Kellerer and developed by Brenner and Hall to explain the LET dependent inverse dose rate effect for oncogenic transformation.

NASA Discipline Radiation Health↗