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Kinematic Sensors Evaluation for Spaceflight Exercise Data Collections

INTRODUCTION: On the International Space Station (ISS), exercise feedback from astronauts is very important to diagnose and mitigate any form-related injuries and ensure efficacious exercise prescriptions and systems. Going forward, exploration exercise efforts seek to gain further quantitative data of human and system performance. Currently, methods of collecting in-flight exercise data on the ISS are limited to marker-based motion capture (MoCap) where astronauts must wear reflective markers over their clothes and specialized cameras are used. The main objective of this work was to investigate the following alternative tracking options: markerless video-based MoCap and inertial measurement units (IMUs). These were compared against traditional marker-based MoCap to evaluate kinematic accuracy and inform feasible methods for future exercise data collections on the ISS, especially in support of future Vibration Isolation and Stabilization (VIS) system development. METHODS: Three test subjects performed a variety of flight-like resistance and aerobic exercises using the Miniature Exercise Device (MED-2), Concept-2 rowing ergometer, barbell mockup, bench (e.g., for bench press, hip thruster, and cycling), and a custom structure for dips. These were intended also to represent exercises which could be performed on the multi-modality European Enhanced Exploration Exercise Device (E4D) [1]. The marker-based MoCap data, collected through a 16-camera OptiTrack MoCap system, was regarded as the gold standard to compare the data against. Passive markers were affixed to each subject according to a modified full body Plug-in Gait marker set [2] with 46 total markers. The markerless MoCap data was collected using two GoPro Hero7 cameras and one GoPro Hero11 camera. For the IMU data, a full body set of 17 Xsens DOTs were placed on the subject: 10 upper body and 7 lower body IMUs. Biomechanical modeling and evaluation was conducted through OpenSim [3] (MoCap), OpenSense [4] (IMU), OpenCap [5] (markerless), ENABLE [6] (markerless), and other modeling software. Secondary objectives included comparing the volume of equipment, reducing mass and crew set-up time. RESULTS AND DISCUSSION: While there were issues with initial processing for the IMUs and markerless MoCap, the results aided in the understanding of each sensor, developing end-to-end processes, and identifying future needs. Some observed concerns with the markerless MoCap approaches included being cognizant of a cluttered background, number of people in field of view, camera number and placement. Some challenges with the IMUs included possible sliding, early deactivation possibly due to exercise pose, and large quantity sensor synchronization. Overall, the markerless MoCap option may be the preferred method of data collection and processing as it provides a solution for certain IMU shortcomings and may be least in equipment volume, upmass, and crew setup time. CONCLUSIONS: While this work was mainly focused on ISS data collection, these sensor data along with continued evaluation and development efforts will help to establish best methods for exercise data collection on Gateway, for other Artemis missions, and beyond. Details on the latest end-to-end processing of the data and results will be presented, along with lessons learned and recommended sensor selection and methods.

S. Faragalla↗

Kinematic Sensors Evaluation for Spaceflight Exercise Data Collections

INTRODUCTION: On the International Space Station (ISS), exercise feedback from astronauts is very important to diagnose and mitigate any form-related injuries and ensure efficacious exercise prescriptions and systems. Going forward, exploration exercise efforts seek to gain further quantitative data of human and system performance. Currently, methods of collecting in-flight exercise data on the ISS are limited to marker-based motion capture (MoCap) where astronauts must wear reflective markers over their clothes and specialized cameras are used. The main objective of this work was to investigate the following alternative tracking options: markerless video-based MoCap and inertial measurement units (IMUs). These were compared against traditional marker-based MoCap to evaluate kinematic accuracy and inform feasible methods for future exercise data collections on the ISS, especially in support of future Vibration Isolation and Stabilization (VIS) system development. METHODS: Three test subjects performed a variety of flight-like resistance and aerobic exercises using the Miniature Exercise Device (MED-2), Concept-2 rowing ergometer, barbell mockup, bench (e.g., for bench press, hip thruster, and cycling), and a custom structure for dips. These were intended also to represent exercises which could be performed on the multi-modality European Enhanced Exploration Exercise Device (E4D) [1]. The marker-based MoCap data, collected through a 16-camera OptiTrack MoCap system, was regarded as the gold standard to compare the data against. Passive markers were affixed to each subject according to a modified full body Plug-in Gait marker set [2] with 46 total markers. The markerless MoCap data was collected using two GoPro Hero7 cameras and one GoPro Hero11 camera. For the IMU data, a full body set of 17 Xsens DOTs were placed on the subject: 10 upper body and 7 lower body IMUs. Biomechanical modeling and evaluation was conducted through OpenSim [3] (MoCap), OpenSense [4] (IMU), OpenCap [5] (markerless), ENABLE [6] (markerless), and other modeling software. Secondary objectives included comparing the volume of equipment, reducing mass and crew set-up time. RESULTS AND DISCUSSION: While there were issues with initial processing for the IMUs and markerless MoCap, the results aided in the understanding of each sensor, developing end-to-end processes, and identifying future needs. Some observed concerns with the markerless MoCap approaches included being cognizant of a cluttered background, number of people in field of view, camera number and placement. Some challenges with the IMUs included possible sliding, early deactivation possibly due to exercise pose, and large quantity sensor synchronization. Overall, the markerless MoCap option may be the preferred method of data collection and processing as it provides a solution for certain IMU shortcomings and may be least in equipment volume, upmass, and crew setup time. CONCLUSIONS: While this work was mainly focused on ISS data collection, these sensor data along with continued evaluation and development efforts will help to establish best methods for exercise data collection on Gateway, for other Artemis missions, and beyond. Details on the latest end-to-end processing of the data and results will be presented, along with lessons learned and recommended sensor selection and methods.

S Faragalla↗

Structure of radio sources with remarkably flat spectra - PKS 0735+178

Three incoherent synchrotron models are used to account for the very flat radio spectrum of the BL Lac object PKS 0735+178. The first interpretation is that the spectrum results from optically-thin synchrotron emission from relativistic electrons with a particular energy distribution. The second posits a single nonuniform, self-absorbed source generated by a wind flowing from a central object. The third suggests that sources with flat spectra are caused by the presence of multiple (three or more) discrete uniform components, and that the magnetic field and relativistic electron distribution for each source is correlated with the size of the individual components. It is proposed that the last model is the most tenable explanation of the phenomenon, and multi-frequency VLBI observations are suggested for the evaluation of the detailed structure of compact radio sources.

Marscher, A. P.↗

Advanced development of atmospheric models

A set of atmospheric analysis and prediction models was developed in support of the SEASAT Program existing objective analysis models which utilize a 125x125 polar stereographic grid of the Northern Hemisphere, which were modified in order to incorporate and assess the impact of (real or simulated) satellite data in the analysis of a two-day meteorological scenario in January 1979. Program/procedural changes included: (1) a provision to utilize winds in the sea level pressure and multi-level height analyses (1000-100 MBS); (2) The capability to perform a pre-analysis at two control levels (1000 MBS and 250 MBS); (3) a greater degree of wind- and mass-field coupling, especially at these controls levels; (4) an improved facility to bogus the analyses based on results of the preanalysis; and (5) a provision to utilize (SIRS) satellite thickness values and cloud motion vectors in the multi-level height analysis.

Kesel, P. G.↗

Exploring the Unknown: Selected Documents in the History of the US Civilian Space Program: Using Space - Volume 3

One of the most important developments of the twentieth century has been the movement of humanity into space with machines and people. The underpinnings of that movement -why it took the shape it did; which individuals and organizations were involved; what factors drove a particular choice of scientific objectives and technologies to be used; and the political, economic, managerial, and international contexts in which the events of the space age unfolded- are all important ingredients of this epoch transition from an earthbound to spacefaring people. This desire to understand the development of spaceflight in the United States sparked this documentary history series. 'Exploring the Unknown' is a multi-volume series containing a selection of key documents in history of the U.S. civil space program. This current volume, Volume III, focusing on the use of space for practical applications, prints 112 key documents on the history of satellite communications, remote sensing of earth, and space as an investment in economic growth, edited for ease of use. Each is introduced by a headnote providing context, bibliographical information, and background information necessary to understanding the document.

Logsdon, John M.↗

Workshop on Science and the Human Exploration of Mars

The exploration of Mars will be a multi-decadal activity. Currently, a scientific program is underway, sponsored by NASA's Office of Space Science in the United States, in collaboration with international partners France, Italy, and the European Space Agency. Plans exist for the continuation of this robotic program through the first automated return of Martian samples in 2014. Mars is also a prime long-term objective for human exploration, and within NASA, efforts are being made to provide the best integration of the robotic program and future human exploration missions. From the perspective of human exploration missions, it is important to understand the scientific objectives of human missions, in order to design the appropriate systems, tools, and operational capabilities to maximize science on those missions. In addition, data from the robotic missions can provide critical environmental data - surface morphology, materials composition, evaluations of potential toxicity of surface materials, radiation, electrical and other physical properties of the Martian environment, and assessments of the probability that humans would encounter Martian life forms. Understanding of the data needs can lead to the definition of experiments that can be done in the near-term that will make the design of human missions more effective. This workshop was convened to begin a dialog between the scientific community that is central to the robotic exploration mission program and a set of experts in systems and technologies that are critical to human exploration missions. The charge to the workshop was to develop an understanding of the types of scientific exploration that would be best suited to the human exploration missions and the capabilities and limitations of human explorers in undertaking science on those missions.

Duke, M. B.↗

Optimizing Orbit-Instrument Configuration for Global Precipitation Mission (GPM) Satellite Fleet

Following the scientific success of the Tropical Rainfall Measuring Mission (TRMM) spearheaded by a group of NASA and NASDA scientists, their external scientific collaborators, and additional investigators within the European Union's TRMM Research Program (EUROTRMM), there has been substantial progress towards the development of a new internationally organized, global scale, and satellite-based precipitation measuring mission. The highlights of this newly developing mission are a greatly expanded scope of measuring capability and a more diversified set of science objectives. The mission is called the Global Precipitation Mission (GPM). Notionally, GPM will be a constellation-type mission involving a fleet of nine satellites. In this fleet, one member is referred to as the "core" spacecraft flown in an approximately 70 degree inclined non-sun-synchronous orbit, somewhat similar to TRMM in that it carries both a multi-channel polarized passive microwave radiometer (PMW) and a radar system, but in this case it will be a dual frequency Ku-Ka band radar system enabling explicit measurements of microphysical DSD properties. The remainder of fleet members are eight orbit-synchronized, sun-synchronous "constellation" spacecraft each carrying some type of multi-channel PMW radiometer, enabling no worse than 3-hour diurnal sampling over the entire globe. In this configuration the "core" spacecraft serves as a high quality reference platform for training and calibrating the PMW rain retrieval algorithms used with the "constellation" radiometers. Within NASA, GPM has advanced to the pre-formulation phase which has enabled the initiation of a set of science and technology studies which will help lead to the final mission design some time in the 2003 period. This presentation first provides an overview of the notional GPM program and mission design, including its organizational and programmatic concepts, scientific agenda, expected instrument package, and basic flight architecture. Following this introduction, we focus specifically on the last topic, that being an analysis which leads to an optimal flight architecture dictated in part by science requirements but constrained by allowable orbital mechanics, instrument scan patterns, and antenna aperture properties. Because the optimal architecture involves an interplay between orbit mechanics and instrument specifications, it is important to recognize that in attempting to serve various scientific themes, the final optimal architecture will represent a compromise concerning dynamic range, spatial resolution, sampling interval, pointing, beam coincidence, and measurement uncertainty. Moreover, cost becomes a major factor in seeking the optimal architecture through the pathways of antenna and instrument scan designs, as well as propulsion requirements associated with the orbit heights of various "constellation" members. Although the results presented at the IGARSS-2001 meeting will likely not be the fully refined flight architecture specifications, they are expected to be nearly complete.

Smith, Eric A.↗

Technology Challenges for Deep-Throttle Cryogenic Engines for Space Exploration

Historically, cryogenic rocket engines have not been used for in-space applications due to their additional complexity, the mission need for high reliability, and the challenges of propellant boil-off. While the mission and vehicle architectures are not yet defined for the lunar and Martian robotic and human exploration objectives, cryogenic rocket engines offer the potential for higher performance and greater architecture/mission flexibility. In-situ cryogenic propellant production could enable a more robust exploration program by significantly reducing the propellant mass delivered to low earth orbit, thus warranting the evaluation of cryogenic rocket engines versus the hypergolic bi-propellant engines used in the Apollo program. A multi-use engine. one which can provide the functionality that separate engines provided in the Apollo mission architecture, is desirable for lunar and Mars exploration missions because it increases overall architecture effectiveness through commonality and modularity. The engine requirement derivation process must address each unique mission application and each unique phase within each mission. The resulting requirements, such as thrust level, performance, packaging, bum duration, number of operations; required impulses for each trajectory phase; operation after extended space or surface exposure; availability for inspection and maintenance; throttle range for planetary descent, ascent, acceleration limits and many more must be addressed. Within engine system studies, the system and component technology, capability, and risks must be evaluated and a balance between the appropriate amount of technology-push and technology-pull must be addressed. This paper will summarize many of the key technology challenges associated with using high-performance cryogenic liquid propellant rocket engine systems and components in the exploration program architectures. The paper is divided into two areas. The first area describes how the mission requirements affect the engine system requirements and create system level technology challenges. An engine system architecture for multiple applications or a family of engines based upon a set of core technologies, design, and fabrication approaches may reduce overall programmatic cost and risk. The engine system discussion will also address the characterization of engine cycle figures of merit, configurations, and design approaches for some in-space vehicle alternatives under consideration. The second area evaluates the component-level technology challenges induced from the system requirements. Component technology issues are discussed addressing injector, thrust chamber, ignition system, turbopump assembly, and valve design for the challenging requirements of high reliability, robustness, fault tolerance, deep throttling, reasonable performance (with respect to weight and specific impulse).

Brown, Kendall K.↗

Blade Displacement Measurements of the Full-Scale UH-60A Airloads Rotor

Blade displacement measurements were acquired during a wind tunnel test of the full-scale UH-60A Airloads rotor. The test was conducted in the 40- by 80-Foot Wind Tunnel of the National Full-Scale Aerodynamics Complex at NASA Ames Research Center. Multi-camera photogrammetry was used to measure the blade displacements of the four-bladed rotor. These measurements encompass a range of test conditions that include advance ratios from 0.15 to unique slowed-rotor simulations as high as 1.0, thrust coefficient to rotor solidity ratios from 0.01 to 0.13, and rotor shaft angles from -10.0 to 8.0 degrees. The objective of these measurements is to provide a benchmark blade displacement database to be utilized in the development and validation of rotorcraft computational tools. The methodology, system development, measurement techniques, and preliminary sample blade displacement measurements are presented.

Barrows, Danny A.↗

NASA's Human Lunar Landing Strategy

In response to the 2018 White House Space Policy Directive-1 to lead an innovative and sustainable lunar exploration, and to the Vice President’s March 2019 direction to do so by 2024, NASA is working to establish humanity's presence on and around the Moon by: 1) sending payloads to its surface, 2) assembling the Gateway outpost in orbit, and 3) conducting the first human lunar landings since 1972. NASA’s Artemis program is implementing a multi-faceted and coordinated agency-wide approach with a focus on the lunar South Pole. The Artemis missions will demonstrate new technologies, capabilities and business approaches needed for future exploration, including Mars. Assessing options to accelerate development of systems, NASA is utilizing public-private engagements to develop and demonstrate capabilities that meet the agency’s human space exploration objectives while stimulating the commercial space industry. Utilizing efforts across mission directorates, the Artemis effort will benefit from programs such as the Science Mission Directorate’s Commercial Lunar Payloads Services program and the Space Technology Mission Directorate’s Tipping Point partnerships for Moon and Mars technologies. This paper will discuss the strategic landscape for NASA's exploration campaign, the agency's approach to accessing the lunar surface with an affordable human-rated landing system, current status and role of U.S. industry, and future plans.

Human landing system↗

Final Plenary Session Transcript

Let s start with the report - as you know - when we are talking about flow control it is a multi-disciplinary type of work. So it involves many people and disciplines. This group first discussed the important issues associated with flow control. As you start doing flow control what are the issues to which you really have to start paying attention? That is the first part I am going to present. Then in the second part I will present some challenges - problems that we should really be looking at. So as far as the issues - if you want to control a flow, you really need to understand the flow physics, because anything that you do comes from the flow physics. The design of the controllers, your decisions on the actuators, sensors, reduced order modeling and all of that, would be helped if you understand flow physics. And you have to have a specific objective - what exactly are you controlling? Are you trying to reduce drag, eliminate separation, reduce noise, enhance mixing? So you have to have very specific control objectives. From all the talks we have seen here actuation is extremely important and it is very problem specific. It depends on what problem you are dealing with so you have to design and build actuators for that specific problem. Sensors obviously are very important, especially when you are dealing with feedback control. Consensus was that when you dealing with flow control, you must take an integrated approach; from the beginning you have to take into account every aspect of it and even maybe to modify your experiment, your geometry, to go along with the actuation, sensors and control models. Development of tools is very important in this multi-disciplinary problem. The tools include CFD, reduced order modeling, controller design, understanding and utilizing the instabilities of the flow, etc. So, in order to have success in flow control, we really need to develop these tools.

Gostelow, Paul↗

Multi-level adaptive finite element methods. 1: Variation problems

A general numerical strategy for solving partial differential equations and other functional problems by cycling between coarser and finer levels of discretization is described. Optimal discretization schemes are provided together with very fast general solvers. It is described in terms of finite element discretizations of general nonlinear minimization problems. The basic processes (relaxation sweeps, fine-grid-to-coarse-grid transfers of residuals, coarse-to-fine interpolations of corrections) are directly and naturally determined by the objective functional and the sequence of approximation spaces. The natural processes, however, are not always optimal. Concrete examples are given and some new techniques are reviewed. Including the local truncation extrapolation and a multilevel procedure for inexpensively solving chains of many boundary value problems, such as those arising in the solution of time-dependent problems.

Brandt, A.↗

Multi-Partner Experiment to Test Volcanic-Ash Ingestion by a Jet Engine

A research team of U.S. Government agencies and engine manufacturers are designing an experiment to test volcanic-ash ingestion by a NASA owned F117 engine that was donated by the U.S. Air Force. The experiment is being conducted under the auspices of NASA s Vehicle Integrated Propulsion Research (VIPR) Program and will take place in early 2014 at Edwards AFB in California as an on-ground, on-wing test. The primary objectives are to determine the effect on the engine of several hours of exposure to low to moderate ash concentrations, currently proposed at 1 and 10 mg/m3 and to evaluate the capability of engine health management technologies for detecting these effects. A natural volcanic ash will be used that is representative of distal ash clouds many 100's to approximately 1000 km from a volcanic source i.e., the ash should be composed of fresh glassy particles a few tens of microns in size. The glassy ash particles are expected to soften and become less viscous when exposed to the high temperatures of the combustion chamber, then stick to the nozzle guide vanes of the high-pressure turbine. Numerous observations and measurements of the engine s performance and degradation will be made during the course of the experiment, including borescope and tear-down inspections. While not intended to be sufficient for rigorous certification of engine performance when ash is ingested, the experiment should provide useful information to aircraft manufacturers, airline operators, and military and civil regulators in their efforts to evaluate the range of risks that ash hazards pose to aviation.

Lekki, John↗

Unsteady numerical simulations of the stability and dynamics of flames

In this report we describe the research performed at the Naval Research Laboratory in support of the NASA Microgravity Science and Applications Program over the past three years (from Feb. 1992) with emphasis on the work performed since the last microgravity combustion workshop. The primary objective of our research is to develop an understanding of the differences in the structure, stability, dynamics and extinction of flames in earth gravity and in microgravity environments. Numerical simulations, in which the various physical and chemical processes can be independently controlled, can significantly advance our understanding of these differences. Therefore, our approach is to use detailed time-dependent, multi-dimensional, multispecies numerical models to perform carefully designed computational experiments. The basic issues we have addressed, a general description of the numerical approach, and a summary of the results are described in this report. More detailed discussions are available in the papers published which are referenced herein. Some of the basic issues we have addressed recently are (1) the relative importance of wall losses and gravity on the extinguishment of downward-propagating flames; (2) the role of hydrodynamic instabilities in the formation of cellular flames; (3) effects of gravity on burner-stabilized flames, and (4) effects of radiative losses and chemical-kinetics on flames near flammability limits. We have also expanded our efforts to include hydrocarbon flames in addition to hydrogen flames and to perform simulations in support of other on-going efforts in the microgravity combustion sciences program. Modeling hydrocarbon flames typically involves a larger number of species and a much larger number of reactions when compared to hydrogen. In addition, more complex radiation models may also be needed. In order to efficiently compute such complex flames recent developments in parallel computing have been utilized to develop a state-of-the-art parallel flame code. This is discussed below in some detail after a brief discussion of the numerical models.

Kailasanath, K.↗

Galactic Superluminal Sources

A new class of X-ray sources was clearly established with the discovery of highly relativistic radio jets from the two galactic sources GRS 1915+105 and GRO Jl655-40. Both of these objects have given us a broader view of black holes and the formation of jets, yet they also show the complexity of the accretion environment near relativistic objects. The fast apparent motion of the jets, their luminosity and variability, their high energy spectrum, and approximately scaling to the behavior of active galactic nuclei, certainly warrant the description "microquasar". A review of the observational data on these sources is presented, and where we stand on a physical picture of GRS 1915+105 and GRO J165540 as taken from multi-wavelength studies is also discussed. Other galactic sources which share some of the properties of the microquasars, and what to look for as a high energy "signature" in future observations is also discussed.

Harmon, B. Alan↗

An Overview and Status of NASA's Radioisotope Power Conversion Technology NRA

NASA's Advanced Radioisotope Power Systems (RPS) development program is developing next generation radioisotope power conversion technologies that will enable future missions that have requirements that can not be met by either photovoltaic systems or by current Radioisotope Power System (RPS) technology. The Advanced Power Conversion Research and Technology project of the Advanced RPS development program is funding research and technology activities through the NASA Research Announcement (NRA) 02-OSS-01, "Research Opportunities in Space Science 2002" entitled "Radioisotope Power Conversion Technology" (RPCT), August 13, 2002. The objective of the RPCT NRA is to advance the development of radioisotope power conversion technologies to provide significant improvements over the state-of-practice General Purpose Heat Source/Radioisotope Thermoelectric Generator by providing significantly higher efficiency to reduce the number of radioisotope fuel modules, and increase specific power (watts/kilogram). Other Advanced RPS goals include safety, long-life, reliability, scalability, multi-mission capability, resistance to radiation, and minimal interference with the scientific payload. Ten RPCT NRA contracts were awarded in 2003 in the areas of Brayton, Stirling, thermoelectric (TE), and thermophotovoltaic (TPV) power conversion technologies. This paper will provide an overview of the RPCT NRA, and a brief summary of accomplishments over the first 18 months but focusing on advancements made over the last 6 months.

Anderson, David J.↗

An Overview and Status of NASA's Radioisotope Power Conversion Technology NRA

NASA's Advanced Radioisotope Power Systems (RPS) development program is developing next generation radioisotope power conversion technologies that will enable future missions that have requirements that can not be met by either photovoltaic systems or by current Radioisotope Power System (RPS) technology. The Advanced Power Conversion Research and Technology project of the Advanced RPS development program is funding research and technology activities through the NASA Research Announcement (NRA) 02- OSS-01, "Research Opportunities in Space Science 2002" entitled "Radioisotope Power Conversion Technology" (RPCT), 13 August 2002. The objective of the RPCT NRA is to advance the development of radioisotope power conversion technologies to provide significant improvements over the state-of-practice General Purpose Heat Source/Radioisotope Thermoelectric Generator by providing significantly higher efficiency to reduce the number of radioisotope fuel modules, and increase specific power (watts/kilogram). Other Advanced RPS goals include safety, long-life, reliability, scalability, multi-mission capability, resistance to radiation, and minimal interference with the scientific payload. These advances would enable a factor of 2 to 4 decrease in the amount of fuel required to generate electrical power. The RPCT NRA selected advanced RPS power conversion technology research and development proposals in the following three areas: innovative RPS power conversion research, RPS power conversion technology development in a nominal 100We scale; and, milliwatt/multi-watt RPS (mWRPS) power conversion research. Ten RPCT NRA contracts were awarded in 2003 in the areas of Brayton, Stirling, thermoelectric (TE), and thermophotovoltaic (TPV) power conversion technologies. This paper will provide an overview of the RPCT NRA, and a brief summary of accomplishments over the first 18 months but focusing on advancements made over the last 6 months.

Anderson, David J.↗

Prokaryotic and Fungal Characterization of the Facilities Used to Assemble, Test, and Launch the OSIRIS-REx Spacecraft

To characterize the ATLO (Assembly, Test, and Launch Operations) environment of the OSIRIS-REx spacecraft, we analyzed 17 aluminum witness foils and two blanks for bacterial, archaeal, fungal, and arthropod DNA. Under NASA’s Planetary Protection guidelines, OSIRIS-REx is a Category II outbound, Category V unrestricted sample return mission. As a result, it has no bioburden restrictions. However, the mission does have strict organic contamination requirements to achieve its primary objective of returning pristine carbonaceous asteroid regolith to Earth. Its target, near-Earth asteroid (101955) Bennu, is likely to contain organic compounds that are biologically available. Therefore, it is useful to understand what organisms were present during ATLO as part of the larger contamination knowledge effort—even though it is unlikely that any of the organisms will survive the multi-year deep space journey. Even though these samples of opportunity were not collected or preserved for DNA analysis, we successfully amplified bacterial and archaeal DNA (16S rRNA gene) from 16 of the 17 witness foils containing as few as 7 ± 3 cells per sample. Fungal DNA (ITS1) was detected in 12 of the 17 witness foils. Despite observing arthropods in some of the ATLO facilities, arthropod DNA (COI gene) was not detected. We observed 1,009 bacterial and archaeal sOTUs (sub-operational taxonomic units, 100% unique) and 167 fungal sOTUs across all of our samples (25–84 sOTUs per sample). The most abundant bacterial sOTU belonged to the genus Bacillus. This sOTU was present in blanks and may represent contamination during sample handling or storage. The sample collected from inside the fairing just prior to launch contained several unique bacterial and fungal sOTUs that describe previously uncharacterized potential for contamination during the final phase of ATLO. Additionally, fungal richness (number of sOTUs) negatively correlates with the number of carbon-bearing particles detected on samples. The total number of fungal sequences positively correlates with total amino acid concentration. These results demonstrate that it is possible to use samples of opportunity to characterize the microbiology of low-biomass environments while also revealing the limitations imposed by sample collection and preservation methods not specifically designed with biology in mind.

Aaron B. Regberg↗