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At least 127 records · Page 7

A Novel Telometric Metric for In-Situ Measurement of Intrauterine Pressure (IUP) in Pregnant and Parturient Rats

During labor and birth, considerable forces exerted on fetuses help instigate certain adaptive postpartum responses (viz., breathing and suckling). To make precise, reliable measures of the forces experienced by rat fetuses during parturition, we developed a novel method for measuring intrauterine pressure (IUP) in late pregnant rats. A small (1.25 x 4cm) telemetric blood pressure sensor is fitted within a fluid-filled balloon, similar in size to a full term rat fetus. The balloon is surgically implanted in the uterus on Gestational Day 19 of the rats' 22-day pregnancy. During birth, dams are able to deliver their pups and the balloon. IUP arsenals are recorded during labor (G22 or 23) and birth. Data derived from a group of implanted rats indicated that pressures on the balloon increased across the period of birth, reaching 18 mmHg during labor, 25 mmHg during pup births and 39 mmHg just prior to delivery of the balloon. These data are within the range reported for conventional IUP measurement techniques. Dams are simultaneously videotaped, enabling us to analyze behavioral expressions of labor contractions and to integrate in-situ and behavioral findings.

Baer, Lisa A.↗

Correlative Analysis of Behavioral and Physiological Concomitants of Labor in Pregnant Rats

During parturition, rats exhibit characteristic behavioral expressions of labor. Lordosis contractions, consisting of an elongation of the dams body, are observed beginning several hours prior to neonate births, whereas vertical contractions, repeated rapid lifts of the abdomen, occur immediately preceding the birth of each neonate. We analyzed underlying changes in intrauterine pressure (IUP) using a telemetric sensor that we modified for use in freely-moving rats. This technique enabled us to correlate behavioral expressions of labor contractions with IUP. A small telemetric blood pressure sensor was fitted within a fluid-filled balloon, similar in size to a full term rat fetus. On Gestational day 19 of the rats' 22-day pregnancy, a unit was surgically implanted within the uterus. The dams were simultaneously videotaped, enabling us to directly correlate IUP signals with behavioral expressions of labor contractions. Earlier phases of labor, consisting predominantly of lordosis contractions were characterized by lower pressures relative to later phases during which higher pressures and vertical contractions were frequently observed.

Baer, L. A.↗

Nonhereditary enhancement of progeny growth

The im electroporated injection of a protease-resistant GH-releasing hormone cDNA into rat dams at 16 d gestation resulted in enhanced long-term growth of the F(1) offspring. The offspring were significantly heavier by 2 wk of age, and the difference was sustained to 10 wk of age. Consistent with their augmented growth, the plasma IGF-I concentration of the F(1) progeny was increased significantly. The pituitary gland of the offspring was significantly heavier and contained an increased number of somatotrophs and PRL-secreting cells, which is indicative of modification of cell lineage differentiation. These unique findings demonstrate that enhanced GH-releasing hormone expression in pregnant dams can result in intergenerational growth promotion by altering development of the pituitary gland in the offspring.

Non-NASA Center↗

Ontogenetic, gravity-dependent development of rat soleus muscle

We tested the hypothesis that rat soleus muscle fiber growth and changes in myosin phenotype during the postnatal, preweaning period would be largely independent of weight bearing. The hindlimbs of one group of pups were unloaded intermittently from postnatal day 4 to day 21: the pups were isolated from the dam for 5 h during unloading and returned for nursing for 1 h. Control pups were either maintained with the dam as normal or put on an alternating feeding schedule as described above. The enlargement of mass (approximately 3 times), increase in myonuclear number (approximately 1.6 times) and myonuclear domain (approximately 2.6 times), and transformation toward a slow fiber phenotype (from 56 to 70% fibers expressing type I myosin heavy chain) observed in controls were inhibited by hindlimb unloading. These properties were normalized to control levels or higher within 1 mo of reambulation beginning immediately after the unloading period. Therefore, chronic unloading essentially stopped the ontogenetic developmental processes of 1) net increase in DNA available for transcription, 2) increase in amount of cytoplasm sustained by that DNA pool, and 3) normal transition of myosin isoforms that occur in some fibers from birth to weaning. It is concluded that normal ontogenetic development of a postural muscle is highly dependent on the gravitational environment even during the early postnatal period, when full weight-bearing activity is not routine.

Non-NASA Center↗

Effects of hindlimb unloading on neuromuscular development of neonatal rats

We hypothesized that hindlimb suspension unloading of 8-day-old neonatal rats would disrupt the normal development of muscle fiber types and the motor innervation of the antigravity (weightbearing) soleus muscles but not extensor digitorum longus (EDL) muscles. Five rats were suspended 4.5 h and returned 1.5 h to the dam for nursing on a 24 h cycle for 9 days. To control for isolation from the dam, the remaining five littermates were removed on the same schedule but not suspended. Another litter of 10 rats housed in the same room provided a vivarium control. Fibers were typed by myofibrillar ATPase histochemistry and immunostaining for embryonic, slow, fast IIA and fast IIB isomyosins. The percentage of multiple innervation and the complexity of singly-innervated motor terminal endings were assessed in silver/cholinesterase stained sections. Unique to the soleus, unloading accelerated production of fast IIA myosin, delayed expression of slow myosin and retarded increases in standardized muscle weight and fiber size. Loss of multiple innervation was not delayed. However, fewer than normal motor nerve endings achieved complexity. Suspended rats continued unloaded hindlimb movements. These findings suggest that motor neurons resolve multiple innervation through nerve impulse activity, whereas the postsynaptic element (muscle fiber) controls endplate size, which regulates motor terminal arborization. Unexpectedly, in the EDL of unloaded rats, transition from embryonic to fast myosin expression was retarded. Suspension-related foot drop, which stretches and chronically loads EDL, may have prevented fast fiber differentiation. These results demonstrate that neuromuscular development of both weightbearing and non-weightbearing muscles in rats is dependent upon and modulated by hindlimb loading.

Non-NASA Center↗

Real-Time Global Flood Estimation Using Satellite-Based Precipitation and a Coupled Land Surface and Routing Model

A widely used land surface model, the Variable Infiltration Capacity (VIC) model, is coupled with a newly developed hierarchical dominant river tracing-based runoff-routing model to form the Dominant river tracing-Routing Integrated with VIC Environment (DRIVE) model, which serves as the new core of the real-time Global Flood Monitoring System (GFMS). The GFMS uses real-time satellite-based precipitation to derive flood monitoring parameters for the latitude band 50 deg. N - 50 deg. S at relatively high spatial (approximately 12 km) and temporal (3 hourly) resolution. Examples of model results for recent flood events are computed using the real-time GFMS (http://flood.umd.edu). To evaluate the accuracy of the new GFMS, the DRIVE model is run retrospectively for 15 years using both research-quality and real-time satellite precipitation products. Evaluation results are slightly better for the research-quality input and significantly better for longer duration events (3 day events versus 1 day events). Basins with fewer dams tend to provide lower false alarm ratios. For events longer than three days in areas with few dams, the probability of detection is approximately 0.9 and the false alarm ratio is approximately 0.6. In general, these statistical results are better than those of the previous system. Streamflow was evaluated at 1121 river gauges across the quasi-global domain. Validation using real-time precipitation across the tropics (30 deg. S - 30 deg. N) gives positive daily Nash-Sutcliffe Coefficients for 107 out of 375 (28%) stations with a mean of 0.19 and 51% of the same gauges at monthly scale with a mean of 0.33. There were poorer results in higher latitudes, probably due to larger errors in the satellite precipitation input.

global flood estimation↗

Gas Supersaturation May Reduce the Survival of Yearling Chinook Salmon in the Lower Columbia River and Ocean Plume

Unusually high flows in the Columbia River in 2011 raised total dissolved gas (TDG) levels in the river above the 120 percent legal limit imposed to prevent harmful impacts to aquatic organisms. This provided a unique opportunity to evaluate the effect on smolt survival. In-river (IR) migrating juvenile yearling Chinook released at Bonneville Dam with acoustic tags during periods when TDG exceeded 120 percent received estimated maximum exposures of 134 TDG. Subsequent daily survival rates in the lower river and plume were reduced by 0.06 per day (SE equals 0.01) and 0.15 per day (SE equals 0.05) relative to IR migrant fish released when TDG was less than 120 percent. Transported smolts (T) released 10-13 kilometers below Bonneville Dam had lower maximum exposure levels (126 percent) and experienced no difference in daily survival rates relative to unexposed smolts. River temperature levels and trends in turbidity and disease prevalence between releases of high and low exposure smolts were not consistent with the observed effects on survival rates. We conclude that smolts may suffer from chronic effects of elevated TDG exposure while migrating through the Columbia River and plume. Consideration should be given to measuring these survival losses in an explicit experimental framework that isolates possible confounding factors.

survival↗

Hydrological Modeling of the Jezero Crater Outlet-Forming Flood

Abundant evidence exists for lakes on Mars both from orbital observations [e.g., 1-3] and in situ exploration [e.g., 4-5]. These lakes can be divided into two classes: those that were hydrologically closed, so their source valley(s) terminated at the basin [3], and those that were hydrologically open, where there was sufficient flow from inlet valley(s) to cause the lake to breach and form an outlet valley [2]. It is easier to be confident from orbital data alone that a standing body of water must have existed in open basins, because there is no other way for their perched outlet valleys to form. The majority of basins fed by valley networks, rather than by isolated inlet valleys, are open [6], with some important exceptions (e.g., Gale Crater). Jezero crater (Fig. 1) is one of the most well-studied open basin paleolakes on Mars, with a breach that re-mains well above the lowest part of the crater floor, and two sedimentary fans at its northwestern margin that are likely deltaic in origin [7-9]. CRISM observations of these sediments indicate they host a variety of alteration minerals [9-11], including smectite and carbonate, and both the mineralogy of the sediments and their settings suggest they have a strong potential for preserving organic materials [10]. As a result, Jezero is a strong candidate landing site for the Mars 2020 rover. Approximate formative discharges have been estimated for its well-preserved western fan (Q approximately 500m3/s) [7], but to our knowledge, no estimates for the dis-charges associated with formation and incision of its outlet valley have been presented. Indeed, only a few studies [e.g., 12-14] have attempted to reconstruct the formation of outlet breaches broadly similar to Jezero anywhere on Mars, despite the apparent commonality of basins with large outlets [e.g., 2]. The outlet valley formed as a dam breach when the lake overflowed. In such an event, the growth and incision of the breach is directly coupled to flood discharge. In the case of Jezero, the discharge through the breach eventually lacked the energy needed to erode through the dam further, preventing complete drainage of the lake. After the initial flood, further incision can take place if additional water flows into, and thus out of, the hydrologically open lake, though the rate of this erosion occurs under more typical fluvial conditions. Despite this qualitative understanding of the process, it is useful to explore numerically what range of model parameters are potentially consistent with obser-vations of the outlet. We ultimately seek to address questions that include: (1) What was the flood hydro-graph?, (2) What sediment transport processes were involved and what can we infer about the erosion process? (3) Can most or all of the Jezero outlet's morphology be explained as a consequence of catastrophic formation, or is additional longer-term erosion required?

geology↗

Effects of 2G on Gene Expression of Stress-Related Hormones in Rat Placenta

Understanding the effects of spaceflight on mammalian reproductive and developmental physiology is important to future human space exploration and permanent settlement beyond Earth orbit. Fetal developmental programming, including modulation of the HPA axis, is thought to originate at the placental-uterine interface, where both transfer of maternal hormones to the fetus and synthesis of endogenous hormones occurs. In healthy rats, fetal corticosterone levels are kept significantly lower by 11BetaHSD-2, which inactivates corticosterone by conversion into cortisone. Placental tissues express endogenous HPA axis-associated hormones including corticotropin-releasing hormone (CRH), pre-opiomelanocortin (POMC), and vasopressin, which may contribute to fetal programming alongside maternal hormones. DNA methylase 3A, 11BetaHSD-2, and 11BetaHSD-1, which are involved in the regulation of maternal cortisol transfer and modulation of the HPA axis, are also expressed in placental tissues along with glucocorticoid receptor and may be affected by differential gravity exposure during pregnancy. Fetuses may respond differently to maternal glucocorticoid exposure during gestation through sexually dimorphic expression of corticosterone-modulating hormones. To elucidate effects of altered gravity on placental gene expression, here we present a ground-based analogue study involving continuous centrifugation to produce 2g hypergravity. We hypothesized that exposure to 2g would induce a decrease in 11BetaHSD-2 expression through the downregulation of DNA methylase 3a and GC receptor, along with concurrent upregulation in endogenous CRH, POMC, and vasopressin expression. Timed pregnant female rats were exposed to 2G from Gestational day 6 to Gestational day 20, and comparisons made with Stationary Control (SC) and Vivarium Control (VC) dams at 1G. Dams were euthanized and placentas harvested on G20. We homogenized placental tissues, extracted and purified RNA, synthesized cDNA, and quantified the expression levels of the genes of interest relative to the GAPDH housekeeping gene, using RT-qPCR and gene-specific cDNA probes. Elucidation of glucocorticoid transfer and synthesis in the placenta can provide new insights into the unique dynamics of mammalian development in microgravity and guide future multi-generational studies in space.

Benson, S.↗

Massachusetts Water Resources: Assessing Flood Events Resulting from North American Beaver Reintroduction with NASA Earth Observations to Inform Biodiversity and Infrastructure Management

North American beavers (Castor canadensis) are returning to Massachusetts after overhunting decimated their populations in the 1700s. Current regulations have allowed this species to recolonize, resulting in increasingly prevalent human-beaver conflicts. These ecosystem engineers can quickly change their environment through the creation of dams, leading to floods that can adversely affect human infrastructures, such as basements, roads, or septic systems. Conversely, beaver dams can positively influence their environment, modifying the physical and chemical properties of streams and providing crucial habitats to a variety of wildlife. The 2020 Spring Boston NASA DEVELOP team collaborated with the Massachusetts Audubon Society to support their efforts in monitoring beaver impacts and managing human-beaver conflicts. The project-utilized data from Landsat 5 Thematic Mapper, Landsat 7 Enhanced Thematic Mapper Plus, and Landsat 8 Operational Land Imager to map the spectral signature created from beaver-induced flooding. The team created a tool called Beaver-Flood Event Detector (B-FED) in Google Earth Engine using imagery from 1985 to 2019. Ancillary datasets were incorporated into B-FED that allow the tool to highlight flood events in wetland areas and in situ observations of beaver presence. Beaver observations in or near flooded areas indicated likelihood that the flood was beaver induced. Time series and animations were also produced to display key regions of landscape change across Massachusetts. B-FED will allow the partner to identify and assess potential ecosystem changes and infrastructural impacts from beavers across Massachusetts and inform future management practices.

Water Resources↗

Massachusetts Water Resources: Assessing Flood Events Resulting from North American Beaver Reintroduction with NASA Earth Observations to Inform Biodiversity and Infrastructure Management

North American beavers (Castor canadensis) are returning to Massachusetts after overhunting decimated their populations in the 1700s. Current regulations have allowed this species to recolonize, resulting in increasingly prevalent human-beaver conflicts. These ecosystem engineers can quickly change their environment through the creation of dams, leading to floods that can adversely affect human infrastructures, such as basements, roads, or septic systems. Conversely, beaver dams can positively influence their environment, modifying the physical and chemical properties of streams and providing crucial habitats to a variety of wildlife. The 2020 Spring Boston NASA DEVELOP team collaborated with the Massachusetts Audubon Society to support their efforts in monitoring beaver impacts and managing human-beaver conflicts. The project-utilized data from Landsat 5 Thematic Mapper, Landsat 7 Enhanced Thematic Mapper Plus, and Landsat 8 Operational Land Imager to map the spectral signature created from beaver-induced flooding. The team created a tool called Beaver-Flood Event Detector (B-FED) in Google Earth Engine using imagery from 1985 to 2019. Ancillary datasets were incorporated into B-FED that allow the tool to highlight flood events in wetland areas and in situ observations of beaver presence. Beaver observations in or near flooded areas indicated likelihood that the flood was beaver induced. Time series and animations were also produced to display key regions of landscape change across Massachusetts. B-FED will allow the partner to identify and assess potential ecosystem changes and infrastructural impacts from beavers across Massachusetts and inform future management practices

Water Resources↗

Diagnosing Challenges and Setting Priorities for Sustainable Water Resource Management Under Climate Change

Managing transboundary river basins requires balancing tradeoffs of sustainable water use and coping with climate uncertainty. We demonstrate an integrated approach to exploring these issues through the lens of a social-ecological system, combining remote and in-situ earth observations, hydrologic and climate models, and social surveys. Specifically, we examine how climate change and dam development could impact the Se Kong, Se San and Sre Pok rivers in the Mekong region. We find that climate change will lead to increased precipitation, necessitating a shift in dam operations, from maintaining low flows to reducing flood hazards. We also find that existing water governance systems in Laos, Vietnam, and Cambodia are ill-prepared to address the problem. We conclude that the solution space for addressing these complex issues will be highly constrained unless major deficiencies in transboundary water governance, strategic planning, financial capacity, information sharing, and law enforcement are remedied in the next decades.

Ibrahim Nourein Mohammed↗

Reviewing Benefits and Costs of Hydropower Development Evidence From the Lower Mekong River Basin

In this paper, we examine the benefits and costs of hydropower development in the Mekong River Basin. We compare four major reports—the Mekong River Commission's (MRC's) Basin Development Plan Programme, Phase 2 (BDP2), the Strategic Environmental Assessment of Hydropower on the Mekong Mainstream (SEA), the Study on the Impacts of Mainstream Hydropower on the Mekong River (MDS), and the MRC's recent council study (CS)—in order to provide the basis for a comparative analysis of the major impact evaluation literature on mainstream dam construction in the Mekong River Basin for the period of 2010–2018. The primary objective of the review is to identify points of agreement, disagreement, inconsistency, and knowledge gaps. Both Mekong River Commission reports (BDP2 and CS) suggest extensive economic benefits for proposed hydropower development, whereas the SEA and MDS indicate that the net impact would be negative. The projected impacts of hydropower development on fisheries, sediment flows, and ecosystems vary widely both in economic and biophysical terms. However, all four reports point to decreased food security and loss of local livelihoods for millions of people as major concerns related to dam development. While considerable resources have been devoted to producing these important studies, the lack of standardization across reports, especially assumptions and methodologies for economic impacts, frustrates efforts at meaningful comparison of their findings and precludes the prospect of clear analytical outcomes or policy impacts.

Cumulative impact assessment↗

Miniature stress transducer has directional capability

Miniature stress transducer uses a semiconductive piezoresistive element to detect stress only on specific axes. Measurement of internal mass stress is based on the compressive deformation of the transducer. The device is applicable to constant stress monitoring in building and dam structural parts.

San Miguel, A.↗

Evaluation of feasibility of mapping seismically active faults in Alaska

The author has identified the following significant results. ERTS-1 imagery is proving to be exceptionally useful in delineating structural features in Alaska which have never been recognized on the ground. Previously unmapped features such as seismically active faults and major structural lineaments are especially evident. Among the more significant results of this investigation is the discovery of an active strand of the Denali fault. The new fault has a history of scattered activity and was the scene of a magnitude 4.8 earthquake on October 1, 1972. Of greater significance is the disclosure of a large scale conjugate fracture system north of the Alaska Range. This fracture system appears to result from compressive stress radiating outward from around Mt. McKinley. One member of the system was the scene of a magnitude 6.5 earthquake in 1968. The potential value of ERTS-1 imagery to land use planning is reflected in the fact that this earthquake occurred within 10 km of the site which was proposed for the Rampart Dam, and the fault on which it occurred passes very near the proposed site for the bridge and oil pipeline crossing of the Yukon River.

Gedney, L. D.↗

Effective use of ERTS multisensor data in the Great Plains

The author has identified the following significant results. In the case of rangelands, manipulation of the digitized material from photographic recordings revealed that the mode-seeking program using only one vegetation group gave the best output map. Efficient mapping of this imagery will possibly require the use of narrow band filters. In the case of cropland, the results obtained with the K-class classifier are shown. Using one feature, the highest percent correct classification was obtained using band 6. Using two features, best results were obtained using bands 4 and 6 and 5 and 6. Specific results obtained with various bands are discussed. In the case of land systems, the eroded shale soils located along the Missouri River reservoirs in South Dakota above the Fort Randall and Big Bend Dams are clearly visible on the IR bands of ERTS scene 17 August, 1972, image description number 1025-16551. The appearance of various types of soil formations is described.

Myers, V. I.↗

Remote sensing of permafrost and geological hazards in Alaska

The author has identified the following significant results. The study of the ERTS-1 imagery of Alaska indicates the following: that areas of different topographic expression affecting the distribution and character of permafrost can be distinguished clearly; that on the Arctic North Slope, regional differences in the distribution and character of permafrost-related oriented thaw lakes can be observed; that the distribution of certain types of geologic materials having a significant effect on the character of permafrost can be delineated on a regional scale; and that the resolution of the imagery is adequate to identify large scale geologic hazards such as landslides, glacier-dammed lakes, aufeis fields, etc. The information concerning the distribution and character of permafrost and geologic hazards to the gained in accomplishing the objectives of this project will be an invaluable aid in solving engineering-geologic and environmental problems related to route and site selection for structures such as roads, railroads, pipelines, and large installations; to distribution of natural construction materials; and to construction and maintenance.

Ferrians, O. J., Jr.↗