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At least 127 records · Page 7
All sourcesNASA NTRSDOE OSTI
NASA NTRS2003

Influences of prostanoids and nitric oxide on post-suspension hypotension in female Sprague-Dawley rats

Impairment in cardiovascular functions sometimes manifested in astronauts during standing postflight, may be related to the diminished autonomic function and/or excessive production of endothelium-dependent relaxing factors. In the present study, using the 30 degrees head-down tilt (HDT) model, we compared the cardiovascular and biochemical effects of 7 days of suspension and a subsequent 6-h post-suspension period between suspended and non-suspended conscious female Sprague-Dawley rats. Mean arterial pressure (MAP) and heart rate were measured prior to suspension (basal), daily thereafter, and every 2h post-suspension. Following 7 days of suspension, MAP was not different from their basal values, however, upon release from suspension, MAP was significantly reduced compared to the non-suspended rats. Nitric oxide levels were elevated while thromboxane A(2) levels declined significantly in both plasma and tissue samples following post-suspension. The levels of prostacyclin following post-suspension remained unaltered in plasma and aortic rings but was significantly elevated in carotid arterial rings. Therefore, the post-suspension reduction in mean arterial pressure is due mostly to overproduction of nitric oxide and to a lesser extent prostacyclin.

Non-NASA Center↗
NASA NTRS1999

Effects of nitric oxide synthase inhibition on sympathetically-mediated tachycardia

The aim of the present study was to determine whether inhibition of nitric oxide (NO) synthesis directly alters the tachycardia produced by sympathetically-derived norepinephrine. The NO synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME; 50 micromol/kg, i.v.), produced a marked rise in mean arterial blood pressure. This pressor response was associated with a fall in heart rate which involved the withdrawal of cardiac sympathetic nerve activity. The NO-donor, sodium nitroprusside (5 microg/kg, i.v.), produced a pronounced fall in mean arterial blood pressure but only a minor increase in heart rate. The beta-adrenoceptor agonist, isoproterenol (0.5 micromol/kg, i.v.), and the membrane-permeable cAMP analogue, 8-(4-chlorophenylthiol)-cAMP (10 micromol/kg, i.v.), produced falls in mean arterial blood pressure and pronounced increases in heart rate. The indirectly acting sympathomimetic agent, tyramine (0.5 mg/kg, i.v.), produced a pressor response and a tachycardia. The effects of sodium nitroprusside, tyramine, isoproterenol and 8-(4-chlorophenylthiol)-cAMP on mean arterial blood pressure were not markedly affected by L-NAME. However, the tachycardia produced by these agents was considerably exaggerated in the presence of this NO synthesis inhibitor. These findings suggest that L-NAME potentiates the tachycardia produced by sympathetically-derived norepinephrine. The increased responsiveness to norepinephrine may involve (i) a rapid up-regulation of cardiac beta1-adrenoceptors and cAMP signaling in cardiac pacemaker cells due to the loss of the inhibitory influence of cardiac NO, and (ii) the up-regulation of beta1-adrenoceptor-mediated signal transduction processes in response to the L-NAME-induced withdrawal of cardiac sympathetic nerve activity.

NASA Discipline Regulatory Physiology↗
NASA NTRS1974

Investigation of nitric oxide decomposition in the temperature range 2500-4100 K

The decomposition of nitric oxide has been studied in the temperature range 2500-4100 K using a shock-tube technique. The principle result of the study was the determination of the rate constant (k) for the reaction O + NO yields N + O2. Mixtures of NO and N2O diluted in argon or krypton were heated by incident shock waves and the infrared emission from the fundamental vibration-rotation band of NO at 5.3 microns was used to monitor the time-varying NO concentration. The experimental values of k are fit closely by the expression k = (2.36 times 10 to the 9th power) x T x exp(-38,640/RT) cm cu/mole-sec, which also provides a good fit to previous data at higher and lower temperatures.

Flower, W. L.↗
NASA NTRS1979

Reaction between nitric oxide and ozone in solid nitrogen

Nitrogen dioxide, NO2, is produced when nitric oxide, NO, and ozone, O3, are suspended in a nitrogen matrix at 11-20 K. The NO2 is formed with first-order kinetics, a 12 K rate constant of (1.4 + or - 0.2) x 0.00001/sec, and an apparent activation energy of 106 + or - 10 cal/mol. Isotopic labeling, variation of concentrations, and cold shield experiments show that the growth of NO2 is due to reaction between ozone molecules and NO monomers, and that the reaction is neither infrared-induced nor does it seem to be a heavy atom tunneling process. Reaction is attributed to nearest-neighbor NO.O3 pairs probably held in a specific orientational relationship that affects the kinetic behavior. When the temperature is raised, more such reactive pairs are generated, presumably by local diffusion. Possible mechanisms are discussed.

Lucas, D.↗
NASA NTRS1981

Chemiluminescence of nitric oxide

Measurements of the intensities of the delta and gamma bands of nitric oxide in the nighttime terrestrial thermosphere are presented and used to infer the rate coefficient for the transition from the C 2 Pi to the A 2 Sigma + states. The nightglow spectrum was observed between 1900 and 2300 A at a resolution of 15 A by a rocket-borne scanning 1/4-m spectrometer pointing north at an apogee of 150 km. Progressions of the delta, gamma and epsilon bands are identified on the spectra by the construction of synthetic spectra, and the contributions of resonance fluorescence to the total band intensities are calculated. Finally, the ratio of the sum of the gamma bands for v-prime = 0 to the sum of the delta bands for v-prime = 0 is used to derive a branching ratio of 0.21 + or - 0.04 to the A 2 Sigma + state, which yields a probability for the C-A transition of 5.6 + or - 1.5 x to the 6th/sec.

Sharp, W. E.↗
NASA NTRS1982

Production of nitric oxide by lightning on Venus

The first measurements of the production of nitric oxide (NO) by a laboratory discharge in a simulated Venus atmosphere are presented. The average NO yield over a range of energies was found to be 3.7 + or - 0.7 x 10 to the 15th molecules/joule. Simultaneous measurements of carbon monoxide (CO) resulting from the lightning-induced dissociation of carbon dioxide (CO2) indicated a CO yield of about 4 x 10 to the 17th molecules/joule. These measurements suggest that at and below cloud level, a region where solar ultraviolet radiation cannot penetrate, the dissociation of CO2 by lightning may be a significant source of oxygen atoms. Depending on the assumed value for the total energy dissipated by lightning on Venus, the production of NO by lightning may be a significant sink of atmospheric nitrogen over the history of Venus.

Levine, J. S.↗
NASA NTRS1986

Near-midnight observations of nitric oxide delta- and gamma-band chemiluminescence

Chemiluminescent nightglow emission of the nitric oxide delta and gamma bands was measured from a sounding rocket launched on April 27, 1981, near local midnight. The integrated band emission rates for this near zenith observation above 205 km were less than 10 Rayleighs. The solar zenith angle was 127 deg. The branching ratio from the C2Pi state to the A2Sigma(+) state of NO was determined from comparison of the total emission rate of the delta band system to that of the gamma band system and found to be 0.30 + or - 0.06. The branching ratios within each of the band systems were found to be consistent with previous theoretical and experimental determinations. The vertical atomic nitrogen distribution, derived with the use of a model atmosphere, was found to have a peak density of 2.0 x 10 to the 7th atoms/cu cm at an altitude of 205 km. The analysis of these data indicate the presence of residual NO emission above 270 km at local midnight on the order of 1 Rayleigh of total band emission.

Tennyson, P. D.↗
NASA NTRS1993

Analysis of selected nitric oxide observations in the lower thermosphere by HALOE on UARS

Measurements of nitric oxide mixing ratios have been made by the Halogen Occultation Experiment on the Upper Atmosphere Research Satellite over an altitude range extending from 15 to 130 km on every spacecraft sunrise and sunset event with the exception of about 8 percent downtime. Selected NO profiles from the first few months of observations were examined with emphasis on the relation between geomagnetic disturbances and enhanced NO in the lower thermosphere. During a large geomagnetic storm on November 8-9, 1991, HALOE obtained 15 NO sunrise profiles along about the 50 deg N latitude circle. A factor of three increase in NO mixing ratio above about 115 km at this latitude and a one to two day decay time were observed. The measured NO longitude/altitude cross section on this day shows a longitudinal asymmetry in the NO distribution with a maximum near the geomagnetic pole. From approximately one month's data, a latitude/altitude cross section of zonal mean NO mixing ratio was calculated. An example of such cross sections demonstrates a strong positive correlation between geomagnetic activity and thermospheric NO globally. This auroral forcing has a larger effect on increasing NO at high latitude than at low latitude.

Luo, Mingzhao↗
NASA NTRSDate not supplied

Nitric Oxide Concentrations from Solar Maximum to Solar Minimum Years Derived from SABER

Nitric oxide (NO) is of particular importance in the upper atmosphere nominally the region between 100 km and 250 km, as it plays the role in cooling and regulating the energy budget of the system. The NO cooling rates have been routinely derived for the past two decades from the measurements made by Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) instrument on the Thermosphere Ionosphere Mesosphere Energetics and Dynamics satellite (TIMED) satellite since 2002. However, a comprehensive long-term dataset of global thermospheric NO concentration as a function of local time is currently unavailable. Given that solar activity significantly influences NO abundance on timescales ranging from days to decades, analyzing the behavior of NO concentration during solar storm and solar cycle can provide valuable insights into thermospheric energy balance, improving storm-time and climatological knowledge. In this study, we used newly calculated NO cooling rates and simulations of neutral kinetic temperature and atomic oxygen number density from the MSIS 2.1 model to derive 20-year NO concentration data. The global annual mean NO concentration shows a strong correlation with the solar cycle, with high concentrations and upper boundaries up to 250 km during solar maximum years and lower concentrations and upper boundaries up to ~230 km during solar minimum years. We also compared NO concentrations obtained from SABER measurements and MSIS 2.1 simulations during solar maximum (2014) and solar minimum (2009) years and found good agreement between the two during both day and night.

Ningchao Wang↗
NASA NTRS1999

Rapid tachyphylaxis to hemodynamic effects of PACAP-27 after inhibition of nitric oxide synthesis

The vasodilator effects of pituitary adenylate cyclase-activating polypeptide (PACAP)-27 are subject to tachyphylaxis in rats treated with the nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME). We examined whether this tachyphylaxis could be prevented by administration of the putative endothelium-derived nitrosyl factor S-nitroso-L-cysteine (L-SNC) and whether L-SNC may exert its effects via increases in cGMP levels in vascular smooth muscle. Five doses of PACAP-27 (2 nmol/kg iv) produced pronounced vasodilator responses in saline-treated rats. These responses were not subject to tachyphylaxis. The first injection of PACAP-27 (2 nmol/kg iv) in L-NAME-treated (50 micromol/kg iv) rats produced vasodilator responses similar to those in saline-treated rats, whereas subsequent injections produced progressively smaller responses. The injection of L-SNC (1,200 nmol/kg iv) before each injection of PACAP-27 prevented tachyphylaxis to the Gs protein-coupled receptor agonist in L-NAME-treated rats, whereas equihypotensive doses of the NO donor sodium nitroprusside (100 micrograms/kg iv) did not. The injection of the membrane-permeant cGMP analog 8-(4-chlorophenylthio)guanosine 3',5'-cyclic monophosphate (8-CPT-cGMP; 30 micromol/kg iv) to L-NAME-treated rats restored resting hemodynamic values to pre-L-NAME levels but did not prevent the development of tachyphylaxis to PACAP-27. These results suggest that nitrosyl factors prevent the development of tachyphylaxis to the hemodynamic actions of PACAP-27. These nitrosyl factors may act independently of their ability to generate cGMP in vascular smooth muscle.

NASA Discipline Regulatory Physiology↗
NASA NTRS2001

Cutaneous interstitial nitric oxide concentration does not increase during heat stress in humans

Inhibition of cutaneous nitric oxide (NO) synthase reduces the magnitude of cutaneous vasodilation during whole body heating in humans. However, this observation is insufficient to conclude that NO concentration increases in the skin during a heat stress. This study was designed to test the hypothesis that whole body heating increases cutaneous interstitial NO concentration. This was accomplished by placing 2 microdialysis membranes in the forearm dermal space of 12 subjects. Both membranes were perfused with lactated Ringer solutions at a rate of 2 microl/min. In both normothermia and during whole body heating via a water perfused suit, dialysate from these membranes were obtained and analyzed for NO using the chemiluminescence technique. In six of these subjects, after the heat stress, the membranes were perfused with a 1 M solution of acetylcholine to stimulate NO release. Dialysate from these trials was also assayed to quantify cutaneous interstitial NO concentration. Whole body heating increased skin temperature from 34.6 +/- 0.2 to 38.8 +/- 0.2 degrees C (P < 0.05), which increased sublingual temperature (36.4 +/- 0.1 to 37.6 +/- 0.1 degrees C; P < 0.05), heart rate (63 +/- 5 to 93 +/- 5 beats/min; P < 0.05), and skin blood flow over the membranes (21 +/- 4 to 88 +/- 10 perfusion units; P < 0.05). NO concentration in the dialysate did not increase significantly during of the heat stress (7.6 +/- 0.7 to 8.6 +/- 0.8 microM; P > 0.05). After the heat stress, administration of acetylcholine in the perfusate significantly increased skin blood flow (128 +/- 6 perfusion units) relative to both normothermic and heat stress values and significantly increased NO concentration in the dialysate (15.8 +/- 2.4 microM). These data suggest that whole body heating does not increase cutaneous interstitial NO concentration in forearm skin. Rather, NO may serve in a permissive role in facilitating the effects of an unknown neurotransmitter, leading to cutaneous vasodilation during a heat stress.

Non-NASA Center↗
NASA NTRS1971

Electron-impact excitation of nitric oxide

The absolute cross sections for the excitation of the NO(+) bands, two nitric oxide beta' bands, and several atomic nitrogen multiplets in the vacuum ultraviolet by electron impact on NO were measured over an energy range extending from threshold to 3000 eV. The variation of the dipole transition moment for the NO(+) band system was also determined.

Stone, E. J.↗
NASA NTRSDate not supplied

Nitric Oxide Planar Laser Induced Fluorescence Rotational Thermometry Characterization of A Hypersonic Boundary Layer

Nitric oxide (NO) planar laser-induced fluorescence (PLIF) was performed to determine rotational temperature profiles and fluctuations within a hypersonic boundary layer above the surface of a 2.75° half-angle wedge. The experiments were performed in the Texas A&M University Actively Controlled Expansion (ACE) hypersonic blow-down wind tunnel at Mach = 5.7 and 𝑅𝑒 = 6 × 10 6 /m. The NO was introduced to the flow in the settling chamber of the ACE tunnel and probed using two laser sheets near 226 nm. The resulting NO PLIF fluorescence signal was acquired using in-house software, which simultaneously recorded tunnel conditions. After a Fast Fourier Transform (FFT) blurring and statistical treatment was performed, the preshock temperature was evaluated to be 58 ± 2 K (3.5%), while the turbulent boundary layer temperature near the wall was found to be 350 K with fluctuations on the order of ±25 K (7%). The relative temperature fluctuations were determined to be 3 − 5% in the freestream and peaked at 33% in the turbulent boundary layer. The advantages and disadvantages of seeding NO in the tunnel settling chamber for thermometric PLIF measurements are discussed.

Hypersonics↗
NASA NTRS1985

An intercomparison of nitric oxide measurement techniques

Results from an intercomparison of techniques to measure tropospheric levels of nitric oxide (NO) are discussed. The intercomparison was part of the National Aeronautics and Space Administration's Global Tropospheric Experiment and was conducted at Wallops Island, VA, in July 1983. Instruments intercompared included a laser-induced fluorescence system and two chemiluminescence instruments. The intercomparisons were performed with ambient air at NO mixing ratios ranging from 10 to 60 pptv and NO-enriched ambient air at mixing ratios from 20 to 170 pptv. All instruments sampled from a common manifold. The techniques exhibited a high degree of correlation among themselves and with changes in the NO mixing ratio. Agreement among the three techniques was placed at approximately + or - 30 percent. Within this level of agreement, no artifacts or species interferences were identified.

Hoell, J. M., Jr.↗
NASA NTRS2014

Simultaneous Laser-induced Fluorescence of Nitric Oxide and Atomic Oxygen in the Hypersonic Materials Environment Test System Arcjet Facility

Simultaneous nitric oxide (NO) and atomic oxygen (O) laser induced fluorescence (LIF) experiments were performed in the Hypersonic Materials Environmental Test System (HYMETS) facility at the NASA Langley Research Center. The data serves as an experimental database for validation for chemical and thermal nonequilibrium models used in hypersonic flows. Measurements were taken over a wide range of stagnation enthalpies (6.7 - 18.5 MJ/kg) using an Earth atmosphere simulant with a composition of 75% N2, 20% O2, and 5% Ar (by volume). These are the first simultaneous measurements of NO and O LIF to be reported in literature for the HYMETS facility. The maximum O LIF mean signal intensity was observed at a stagnation enthalpy of approximately 12 MJ/kg while the maximum NO LIF mean signal intensity was observed at a stagnation enthalpy of 6.7 MJ/kg. Experimental results were compared to simple fluorescence model that assumes equilibrium conditions in the plenum and frozen chemistry in the isentropic nozzle expansion (Mach 5). The equilibrium calculations were performed using CANTERA v2.1.1 with 16 species. The fluorescence model captured the correlation in mean O and NO LIF signal intensities over the entire range of stagnation enthalpies tested. Very weak correlations between single-shot O and NO LIF intensities were observed in the experiments at all of the stagnation enthalpy conditions.

Johansen, Craig↗
NASA NTRS2022

Thermospheric Nitric Oxide Cooling Responses to the 14 December 2020 Solar Eclipse

The behaviors of the nitric oxide (NO) cooling in the lower thermosphere during the 14 December 2020 solar eclipse are studied using Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) measurements and WACCM-X simulations. We found that NO cooling rate decreases during the solar eclipse in both SABER measurements and WACCM-X simulations. The maximum decrease of the NO cooling is 40% in SABER measurements and 25% in WACCM-X simulations. The NO cooling process is initiated almost entirely through the collisions with atomic oxygen (O) which depends linearly on NO and O densities and non-linearly on the neutral temperature. During the eclipse, the NO concentration and temperature decreases are larger than that of O concentration. Consequently, the eclipse-time NO concentration and temperature decreases are the major drivers of the NO cooling rate decrease. The decreases of the temperature and the NO concentration contribute comparably to the eclipse-time NO cooling rate decrease.

solar eclipse↗
NASA NTRS2001

Mechanisms of cell signaling by nitric oxide and peroxynitrite: from mitochondria to MAP kinases

Many of the biological and pathological effects of nitric oxide (NO) are mediated through cell signaling pathways that are initiated by NO reacting with metalloproteins. More recently, it has been recognized that the reaction of NO with free radicals such as superoxide and the lipid peroxyl radical also has the potential to modulate redox signaling. Although it is clear that NO can exert both cytotoxic and cytoprotective actions, the focus of this overview are those reactions that could lead to protection of the cell against oxidative stress in the vasculature. This will include the induction of antioxidant defenses such as glutathione, activation of mitogen-activated protein kinases in response to blood flow, and modulation of mitochondrial function and its impact on apoptosis. Models are presented that show the increased synthesis of glutathione in response to shear stress and inhibition of cytochrome c release from mitochondria. It appears that in the vasculature NO-dependent signaling pathways are of three types: (i) those involving NO itself, leading to modulation of mitochondrial respiration and soluble guanylate cyclase; (ii) those that involve S-nitrosation, including inhibition of caspases; and (iii) autocrine signaling that involves the intracellular formation of peroxynitrite and the activation of the mitogen-activated protein kinases. Taken together, NO plays a major role in the modulation of redox cell signaling through a number of distinct pathways in a cellular setting.

Non-NASA Center↗
NASA NTRS2001

L-NAME, a nitric oxide synthase inhibitor, as a potential countermeasure to post-suspension hypotension in rats

A large number of astronauts returning from spaceflight experience orthostatic hypotension. This hypotension may be due to overproduction of vasodilatory mediators, such as nitric oxide (NO) and prostaglandins. To evaluate the role of the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) as a countermeasure against the post-suspension reduction in mean arterial pressure (MAP), we assessed the cardiovascular responses and vascular reactivity to 7-day 30 degrees tail-suspension and a subsequent 6 hr post-suspension period in conscious rats. After a pre-suspension reading, direct MAP and heart rate (HR) were measured daily and every 2 hrs post-suspension. The NO synthase inhibitor L-NAME (20 mg/kg, i.v.), or saline, were administered after the 7th day reading prior to release from suspension and at 2 and 4 hrs post-suspension. At 6 hrs post-suspension, vascular reactivity was assessed. While MAP did not change during the suspension period, it was reduced post-suspension. Heart rate was not significantly altered. L-NAME administration reversed the post-suspension reduction in MAP. In addition, the baroreflex sensitivity for heart rate was modified by L-NAME. Thus, the post-suspension reduction in MAP may be due to overproduction of NO and altered baroreflex activity.

NASA Discipline Cardiopulmonary↗