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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 127 records · Page 7
All sourcesNASA NTRSDOE OSTI
NASA NTRS1984

In vitro seed to seed growth on clinostats

The effect of a long term micro-gravity environment on the life cycle of plants is unknown. Whether higher plants have evolved to a stage where removal or reduction of gravity is detrimental to plant life cycle and thus fatal to the plant species, is an unanswered question in space plants which were successfully grown through the various stages of their life cycle. Attempts to grow plants as a continuous integral process from seed to seed through one generation were successful until recently. Culture of plants through multiple generations was not accomplished in space nor in ground based studies. The effect of long term simulated weightlessness by growing consecutive generations of plants continuously on clinostats using the cruciferous plants, Arabidopsis thaliana (L.) Heyn. and Cardamine oligosperma Nutt. is being investigated.

Hoshizaki, T.↗
NASA NTRS1988

Rapid changes in protein phosphorylation associated with light-induced gravity perception in corn roots

The effect of light and calcium depletion on in vivo protein phosphorylation was tested using dark-grown roots of Merit corn. Light caused rapid and specific promotion of phosphorylation of three polypeptides. Pretreatment of roots with ethylene glycol bis N,N,N',N' tetraacetic acid and A23187 prevented light-induced changes in protein phosphorylation. We postulate that these changes in protein phosphorylation are involved in the light-induced gravity response.

NASA Program Space Biology↗
NASA NTRS2001

Plasma membrane NADH oxidase of maize roots responds to gravity and imposed centrifugal forces

NADH oxidase activities measured with excised roots of dark-grown maize (Zea mays) seedlings and with isolated plasma membrane vesicles from roots of dark-grown maize oscillated with a regular period length of 24 min and were inhibited by the synthetic auxin 2,4-dichlorophenoxyacetic [correction of dichorophenoxyacetic] acid. The activities also responded to orientation with respect to gravity and to imposed centrifugal forces. Turning the roots upside down resulted in stimulation of the activity with a lag of about 10 min. Returning the sections to the normal upright position resulted in a return to initial rates. The activity was stimulated reversibly to a maximum of about 2-fold with isolated plasma membrane vesicles, when subjected to centrifugal forces of 25 to 250 x g for 1 to 4 min duration. These findings are the first report of a gravity-responsive enzymatic activity of plant roots inhibited by auxin and potentially related to the gravity-induced growth response. c2001 Editions scientifiques et medicales Elsevier SAS.

NASA Discipline Cell Biology↗
NASA NTRS1987

The role of calcium ions in gravity signal perception and transduction

Ca2+ is implicated as a messenger in coupling various environmental stimuli, such as gravity and light, to response. In recent years, it has become evident that Ca2+ plays a central role in all three phases of gravitropism--perception, transduction and response. The root cap, which is known to contain high amounts of Ca2+ and calmodulin, is the primary site of gravity perception. The possible role of phosphoinositide turnover and Ca(2+) - and Ca(2+) -calmodulin-dependent enzymes such as Ca(2+) -ATPase and protein kinases in gravitropism is discussed. A model is proposed to describe the role of Ca2+ in both normal and light-dependent gravity response in roots.

NASA Program Space Biology↗
NASA NTRS1991

Advances in space biology and medicine. Vol. 1

Topics discussed include the effects of prolonged spaceflights on the human body; skeletal responses to spaceflight; gravity effects on reproduction, development, and aging; neurovestibular physiology in fish; and gravity perception and circumnutation in plants. Attention is also given to the development of higher plants under altered gravitational conditions; the techniques, findings, and theory concerning gravity effects on single cells; protein crystal growth in space; and facilities for animal research in space.

Bonting, Sjoerd L.↗
NASA NTRS1996

Comparison of Long-Term Storage in Chemical Fixatives on Morphology and Anatomy of Super-Dwarf Wheat

Wheat plants (Triticum aestivum L. cv Super-Dwarf) are grown in the microgravity of space and harvested for morphological and anatomical comparison with those exposed to gravity on earth. Such plants are subjected to relatively long periods of storage in chemical fixatives. Examination, evaluation and verification that the integrity of the vascular system is maintained for extended periods of storage in fixatives are required. McDowell and Trump's [4% Formaldehyde -- 1 % Glutaraldehyde (4F: 1 G)] or Variant I [(Russian Fixative): Formalin: Acetic Acid: Alcohol] fixatives, adjusted to pH 7.2, were placed in Aclam(TM), FilmORap(TM), or FilmOSun(TM) plastic bags on April 4, 1994. Wheat seedlings were harvested on days 9, 28, and 68 and preserved in these fixatives. Subsamples of leaves and/or seeds were taken from these stocks after various times in storage, dehydrated, and embedded in Spurr's, LR White's or Unicryl resin. Semithin (1 mm) and thin (50-70 nm) sections were examined by light and transmission electron microscopy. In a few sections, we have observed a slight plasmolysis of the cytostol in leaf tissue fixed with the Variant I, but overall there seem to be no major artifacts in the anatomical structure. The plasmalemma and other organelles appeared normal in the McDowell and Trump fixative. Use of differential chromophores suggests that LR White or Unicryl resins may give greater flexibility for enzyme localizations at both the light and electron microscopical levels.

Bubenheim, David L.↗
NASA NTRS1992

Life in space

The scope of space life sciences and current research on the physiology of man in space are reviewed by examining Spacelab SLS-1. Milestones of space life sciences are discussed, with emphasis on the Skylab facility, the Space Shuttle program, and the Soviet Mir space station. Attention is given to the topic of the origins of life as it relates to space life sciences. The discovery of amino acids in meteorites and the question of whether the earth was seeded with life from space are discussed. A brief overview of efforts in the search for extraterrestrial intelligence is presented. Consideration is also given to the effects of gravity on cells, the effects of radiation, plant biology, CELSS, and the effects of gravity on humans.

West, John B.↗
NASA NTRS1989

How to detect when cells in space perceive gravity

It is useful to be able to measure when and whether cells detect gravity during spaceflights. For studying gravitational physiology, gravity perception is the response the experimentalist needs to measure. Also, for growing plants in space, plant cells may have a non-directional requirement for gravity as a development cue. The main goals of spaceflight experiments in which gravity perception would be measured are to determine the properties of the gravity receptor and how it is activated, and to determine fundamental characteristics of the signal generated. The main practical difficulty with measuring gravity sensing in space is that gravity sensing cannot be measured with certainty on earth. Almost all experiments measure gravitropic curvature. Reciprocity and intermittent stimulation are measurements which were made to some degree on earth using clinostatting, but which would provide clearer results if done with microgravity rather than clinostatting. These would be important uses of the space laboratory for determining the nature of gravity sensing in plants. Those techniques which do not use gravitropic curvature to measure gravity sensing are electrophysiological. The vibrating probe would be somewhat easier to adapt to space conditions than the intracellular microelectrode because it can be positioned with less precision. Ideally, a non-invasive technique would be best suited if an appropriate measure could be developed. Thus, the effect of microgravity on cultured cells is more likely to be by large-scale physical events than gravity sensing in the culture cells. It is not expected that it will be necessary to determine whether individual cultured cells perceive gravity unless cells grow abnormally even after the obvious microgravity effects on the culture as a whole can be ruled out as the cause.

Bjoerkman, Thomas↗
NASA NTRSDate not supplied

Novel Hardware for a Lunar Plant Experiment

We propose an experiment, LPX (Lunar plant growth experiment), on the surface of the Moon that will conduct a fundamental investigation into the response of the plant Arabidopsis to lunar gravity and lunar radiation. For statistical significance, we will include more than 50 seeds. The experiment begins after lunar landing with water added to the seed platform and continues for 10 days or more. The growth of the plants, reaching ~ 1 cm high with typically 3-4 leaves in 10 days, will be monitored by camera and the carbon dioxide in the sealed chamber also will be monitored. The hardware for the LPX is based on proven technology flown on the ISS in Dec 2019 (Kitto et al. 2021, Life Sci. Space Res. in press) and the “Phytofuge” canister for Techshot MVP (Multi-use Variable-gravity Platform) centrifuge – both on ISS now. Both of these systems are TRL 9. This approach will allow identical units to be sent to the Moon and to be flown on ISS - allowing direct comparisons to partial gravity experiments done on the ISS experiment. Germination and growth of Arabidopsis requires temperature range of 20–25°C with the optimum being 22–23°C (Shymanovich and Kiss 2020, Gravit. & Space Res., 8, 1-7). This level of thermal control is a challenge on a small lunar lander and is effectively set by the latitude of the landing site. Given the mass and power limitations on a small lander, and the internal power of the LPX (~ 5W) the landing site needs to be between 73° and 77° − for which the noon time lunar surface temperatures are +15°C and -3°C (Hurley et al. 2015, Icarus 255,159-163). Within this range the temperature can be set with a small thermostatically-controlled heater These proposed experiments would be the first to grow plants on another planet and would provide important insights in terms of growth of plants on the Moon as part of bioregenerative life support.

Christopher P Mckay↗
NASA NTRS1983

Summary of Study Group Session Discussions

The relationship between gravitational physiology and calcium metabolism is examined. The role of gravity on the problems of bone response, low gravity environments, calcium in plants, and the potential in animal systems for alterations in nerve and muscle function as variations in extracellular calcium levels occurred are discussed. Innovative materials for experiments on interactions between calcium and gravity, experiments that could utilize ionospheres or calcium-measuring dyes, and specific gravity calcium experiments are also addressed.

Source record↗
NASA NTRS1999

The response of lazy-2 tomato seedlings to curvature-inducing magnetic gradients is modulated by light

Shoots of the lazy-2 mutant of tomato (Lycopersicon esculentum Mill., cv. Ailsa Craig) exhibit negative gravitropism in the dark, but respond positively gravitropically in (red) light. In order to test whether high-gradient magnetic fields (HGMFs) exert only ponderomotive effects on amyloplasts or affect other physiological processes, we induced magnetophoretic curvature in wild-type (WT) and lazy-2 mutant seedlings. Straight hypocotyls of 4-d-old plants were selected and the tips of their hooks were placed in an HGMF near the edge of a magnetized ferromagnetic wedge [grad (H2/2) approximately 10(9)-10(10) Oe2/cm] and mounted on a 1-rpm clinostat. After 4 h in the dark, 85% of WT hypocotyls and 67% of mutant hypocotyls curved toward the wedge. When the seedlings were exposed to red light for 1 h prior to and during the application of the HGMF, 78% of the WT seedlings curved toward the magnetic gradient, but the majority of the lazy-2 seedlings (75%) curved away from the stronger field area. Intracellular amyloplast displacement in the HGMF was similar for both varieties and resembled the displacement after horizontal reorientation. The WT showed a distinct graviresponse pattern depending on the orientation of the hook, even after excision of the apex. Application of HGMFs to decapitated hypocotyls resulted in curvature consistent with that obtained after horizontal reorientation. After light exposure, decapitated lazy-2 seedlings did not respond positively gravitropically. The data imply that the lazy-2 mutants perceive the displacement of amyloplasts in a similar manner to the WT and that the HGMF does not affect the graviresponse mechanism. The study demonstrates that ponderomotive forces due to HGMFs are useful for the analysis of the gravity-sensing mechanism in plants.

NASA Discipline Plant Biology↗
NASA NTRS1984

NASA Space Biology Program. Eighth annual symposium's program and abstracts

The activities included five half days of presentations by space biology principal investigators, an evening of poster session presentations by research associates, and an afternoon session devoted to the Flight Experiments Program. Areas of discussion included the following: gravity receptor mechanisms; physiological effects of gravity, structural mass; fluid dynamics and metabolism; mechanisms of plant response; and the role of gravity in development.

Halstead, T. W.↗
NASA NTRSDate not supplied

Plant Physiological Responses and Production Challenges in Space

This is an overview of the unique plant physiology and crop production challenges that occur when trying to grow plants in a micro gravity spaceflight environment. The goal of this learning session is to raise awareness of these challenges for the space flight research community.

Plant↗
NASA NTRS2019

Plant Water Management (PWM) Experiment

Plant Water Management is a technology demonstration of recent advances in micro-g capillary fluidics research applied to plant growth systems. It has applications in long-term food production systems for missions to the Moon and Mars, as well as the immediate need for ISS food supplements to the crew diet. PWM will demonstrate the low-gravity role of surface tension, wetting, and system geometry to effectively replace the role of gravity in certain terrestrial plant growth systems.

Hatch, Tyler↗
NASA NTRS2002

Root gravitropism in response to a signal originating outside of the cap

We have developed image analysis software linked to a rotating stage, allowing constraint of any user-selected region of a root at a prescribed angle during root gravitropism. This device allows the cap of a graviresponding root to reach vertical while maintaining a selected region within the elongation zone at a gravistimulated angle. Under these conditions gravitropic curvature of roots of Zea mays L. continues long after the root cap reaches vertical, indicating that a signal from outside of the cap can contribute to the curvature response.

Non-NASA Center↗
NASA NTRS2002

Interactions between gravitropism and phototropism in plants

To receive adequate light and nutrients for survival, plants orient stems and stem-like organs toward light and away from the gravity vector and, conversely, orient roots into the soil, away from light toward the direction of gravity. Therefore, both gravity and light can influence the differential growth of plant organs. To add to the complexity of the interactions between gravity and light, each stimulus can enhance or reduce the effectiveness of the other. On earth, the constant presence of gravity makes it difficult to determine whether plant growth and development is influenced by gravity or light alone or the combination of the two stimuli. In the past decade, our understanding of the gravity and light transduction pathways has advanced through the use of mutants in either gravitropic or phototropic responses and the use of innovative techniques that reduce the effects of one stimulus on the other. Thus, both unique and common elements in the transduction pathways of the gravitropic and phototropic responses have been isolated. This article is focused on the interactions between the light- and gravity-transduction pathways and describes methods used to separate the influences of these two environmental stimuli.

NASA Discipline Plant Biology↗