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At least 127 records · Page 7

Modeling the growth dynamics of four candidate crops for Controlled Ecological Life Support Systems (CELSS)

The production of food for human life support for advanced space missions will require the management of many different crops. The research to design these food production capabilities along with the waste management to recycle human metabolic wastes and inedible plant components are parts of Controlled Ecological Life Support Systems (CELSS). Since complete operating CELSS were not yet built, a useful adjunct to the research developing the various pieces of a CELSS are system simulation models that can examine what is currently known about the possible assembly of subsystems into a full CELSS. The growth dynamics of four crops (wheat, soybeans, potatoes, and lettuce) are examined for their general similarities and differences within the context of their important effects upon the dynamics of the gases, liquids, and solids in the CELSS. Data for the four crops currently under active research in the CELSS program using high-production hydroponics are presented. Two differential equations are developed and applied to the general characteristics of each crop growth pattern. Model parameters are determined by closely approximating each crop's data.

Volk, Tyler↗

Controlled ecological life support systems; Proceedings of Workshop II of the 26th COSPAR Plenary Meeting, Toulouse, France, June 30-July 11, 1986

The present conference on the development status of Controlled Ecological Life Support Systems (CELSSs) discusses food production and gas exchange with the Spirulina blue-green alga, biomass recycling for greater energy efficiency in algal culture CELSSs, algal proteins for food processing in a CELSS, a CELSS with photosynthetic N2-fixing cyanobacteria, the NASA CELSS program, and vapor compression ditillation and membrane technology for water revitalization. Also discussed are a fundamental study of CELSS gas monitoring, the application of catalytic wet oxidation to CELSS, a large-scale perspective on ecosystems, Japanese CELSS research activities, the use of potatoes in bioregenerative life-support, wheat production in controlled environments, and a trickle water and feeding system in plant culture.

Macelroy, R. D.↗

Utilization of non-conventional systems for conversion of biomass to food components

The potential use of micro-algae in yielding useful macronutrients for the CELSS is investigated. Algal proteins were isolated and characterized from green algae (Scenedesmus obliquus) grown under controlled conditions. The RNA and DNA contents were determined, and methodology for reduction of the nucleic acid content to acceptable levels developed. Lipid extraction procedures using supercritical fluids were tailored to removal of undesirable lipids and pigments. Initial steps toward preparation of model foods for potential use in the CELSS were taken. The goal was to fabricate food products which contain isolated algal macronutrients such as proteins and lipids and also some components derived from higher plants including wheat flour, soy flour, potato powder (flakes), soy oil, and corn syrup.

Karel, M.↗

Mathematical modeling of control subsystems for CELSS: Application to diet

The dynamic control of a Closed Ecological Life Support System (CELSS) in a closed space habitat is of critical importance. The development of a practical method of control is also a necessary step for the selection and design of realistic subsystems and processors for a CELSS. Diet is one of the dynamic factors that strongly influences, and is influenced, by the operational states of all major CELSS subsystems. The problems of design and maintenance of a stable diet must be obtained from well characterized expert subsystems. The general description of a mathematical model that forms the basis of an expert control program for a CELSS is described. The formulation is expressed in terms of a complete set of time dependent canonical variables. System representation is dynamic and includes time dependent storage buffers. The details of the algorithm are described. The steady state results of the application of the method for representative diets made from wheat, potato, and soybean are presented.

Waleh, Ahmad↗

Multi-frequency and polarimetric radar backscatter signatures for discrimination between agricultural crops at the Flevoland experimental test site

We describe the calibration and analysis of multi-frequency, multi-polarization radar backscatter signatures over an agriculture test site in the Netherlands. The calibration procedure involved two stages: in the first stage, polarimetric and radiometric calibrations (ignoring noise) were carried out using square-base trihedral corner reflector signatures and some properties of the clutter background. In the second stage, a novel algorithm was used to estimate the noise level in the polarimetric data channels by using the measured signature of an idealized rough surface with Bragg scattering (the ocean in this case). This estimated noise level was then used to correct the measured backscatter signatures from the agriculture fields. We examine the significance of several key parameters extracted from the calibrated and noise-corrected backscatter signatures. The significance is assessed in terms of the ability to uniquely separate among classes from 13 different backscatter types selected from the test site data, including eleven different crops, one forest and one ocean area. Using the parameters with the highest separation for a given class, we use a hierarchical algorithm to classify the entire image. We find that many classes, including ocean, forest, potato, and beet, can be identified with high reliability, while the classes for which no single parameter exhibits sufficient separation have higher rates of misclassification. We expect that modified decision criteria involving simultaneous consideration of several parameters increase performance for these classes.

Freeman, A.↗

Diet expert subsystem for CELSS

An account is given of the mathematical basis of a diet-controlling expert system, designated 'Ceres' for the human crews of a Controlled Ecological Life Support System (CELSS). The Ceres methodology can furnish both steady-state and dynamic diet solutions; the differences between Ceres and a conventional nutritional-modeling method is illustrated by the case of a three-component, potato-wheat-soybean food system. Attention is given to the role of food processing in furnishing flexibility in diet-planning management. Crew diet solutions based on simple optimizations are not necessarily the most suitable for optimum CELSS operation.

Yendler, Boris S.↗

Crop growth and associated life support for a lunar farm

Supporting human life on a lunar base will require growing many different food crops. This paper investigates the growth dynamics of four crops (wheat, soybeans, potatoes, and lettuce) for general similarities and differences, along with associated material flows of the gases, liquids, and solids in a lunar farm. The human dietary requirements are compared with the protein, carbohydrate, and lipid contents of these hydroponically grown, high-productivity crops to derive a lunar farm diet. A simple and general analytical model is used to calculate the mass fluxes of CO2, H2O, HNO3, and O2 during the life cycle of each of the four crops. The resulting farm crop areas and corresponding biomass production rates are given. One significant conclusion of this study is that there is a 'lipid problem' associated with the incorporation of these four crops into a viable diet.

Volk, Tyler↗

Closed Ecological Life Support Systems (CELSS) Test Facility

The CELSS Test Facility (CTF) is being developed for installation on Space Station Freedom (SSF) in August 1999. It is designed to conduct experiments that will determine the effects of microgravity on the productivity of higher (crop) plants. The CTF will occupy two standard SSF racks and will accommodate approximately one square meter of growing area and a canopy height of 80 cm. The growth volume will be isolated from the external environment, allowing stringent control of environmental conditions. Temperature, humidity, oxygen, carbon dioxide, and light levels will all be closely controlled to prescribed set points and monitored. This level of environmental control is needed to prevent stress and allow accurate assessment of microgravity effect (10-3 to 10-6 x g). Photosynthetic rates and respiration rates, calculated through continuous recording of gas concentrations, transpiration, and total and edible biomass produced will be measured. Toxic byproducts will be monitored and scrubbed. Transpiration water will be collected within the chamber and recycled into the nutrient solution. A wide variety of crop plants, e.g., wheat, soy beans, lettuce, potatoes, can be accommodated and various nutrient delivery systems and light delivery systems will be available. In the course of its development, the CTF will exploit fully, and contribute importantly, to the state-of-art in closed system technology and plant physiology.

Macelroy, Robert D.↗

Statistical analysis of environmental variability within the CELSS breadboard project's biomass production chamber

Variability in the aerial and root environments of NASA's Breadboard Project's Biomass Production Chamber (BPC) was determined. Data from two lettuce and two potato growouts were utilized. One growout of each crop was conducted prior to separating the upper and lower chambers; the other was subsequent to separation. There were little or no differences in pH, EC, or solution temperature between the upper and lower chamber or within a chamber. Variation in the aerial environment within a chamber was two to three times greater than variation between chambers for air temperature, relative humidity, and PPF. High variability in air velocity, relative to tray position, was observed. Separating the BPC had no effect on PPF, air velocity, solution temperature, pH, or EC. Separation reduced the gradient in air temperature and relative humidity between the upper and lower chambers, but increased the variability within a chamber. Variation between upper and lower chambers was within 5 percent of environmental set-points and of little or no physiological significance. In contrast, the variability within a chamber limits the capability of the BPC to generate statistically reliable data from individual tray treatments at this time.

Stutte, G. W.↗

The dynamics of hydroponic crops for simulation studies of the CELSS initial reference configurations

During the past several years, the NASA Program in Controlled Ecological Life Support Systems (CELSS) has continued apace with crop research and logistic, technological, and scientific strides. These include the CELSS Test Facility planned for the space station and its prototype Engineering Development Unit, soon to be active at Ames Research Center (as well as the advanced crop growth research chamber at Ames); the large environmental growth chambers and the planned human test bed facility at Johnson Space Center; the NSCORT at Purdue with new candidate crops and diverse research into the CELSS components; the gas exchange data for soy, potatoes, and wheat from Kennedy Space Center (KSC); and the high-precision gas exchange data for wheat from Utah State University (USU). All these developments, taken together, speak to the need for crop modeling as a means to connect the findings of the crop physiologists with the engineers designing the system. A need also exists for crop modeling to analyze and predict the gas exchange data from the various locations to maximize the scientific yield from the experiments. One fruitful approach employs what has been called the 'energy cascade'. Useful as a basis for CELSS crop growth experimental design, the energy cascade as a generic modeling approach for CELSS crops is a featured accomplishment in this report. The energy cascade is a major tool for linking CELSS crop experiments to the system design. The energy cascade presented here can help collaborations between modelers and crop experimenters to develop the most fruitful experiments for pushing the limits of crop productivity. Furthermore, crop models using the energy cascade provide a natural means to compare, feature for feature, the crop growth components between different CELSS experiments, for example, at Utah State University and Kennedy Space Center.

Volk, Tyler↗

Ethylene dynamics in the CELSS biomass production chamber

A material balance model for ethylene was developed and applied retrospectively to data obtained in the Biomass Production Chamber of CELSS in order to calculate true plant production rates of ethylene. Four crops were analyzed: wheat, lettuce, soybean, and potato. The model represents an effort to account for each and every source and sink for ethylene in the system. The major source of ethylene is the plant biomass and the major sink is leakage to the surroundings. The result, expressed in the units of ppd/day, were converted to nl of ethylene per gram of plant dry mass per hour and compare favorably with recent glasshouse to belljar experiments.

Rakow, Allen L.↗

Electronic-To-Optical-To-Electronic Packet-Data Conversion

Space-time multiplexer (STM) cell-based communication system designed to take advantage of both high throughput attainable in optical transmission links and flexibility and functionality of electronic processing, storage, and switching. Long packets segmented and transmitted optically by wavelength-division multiplexing. Performs optoelectronic and protocol conversion between electronic "store-and-forward" protocols and optical "hot-potato" protocols.

Monacos, Steve↗

Electrolytic Removal of Nitrate From CELSS Crop Residues

The controlled ecological life support system (CELSS) resource recovery system is a waste processing system using aerobic and anaerobic bioreactors to recover plant nutrients and secondary foods from inedible biomass. Crop residues contain significant amounts of nitrate which presents two problems: (1) both CELSS biomass production and resource recovery consume large quantities of nitric acid, (2) nitrate causes a variety of problems in both aerobic and anaerobic bioreactors. A technique was proposed to remove the nitrate from potato inedible biomass leachate and to satisfy the nitric acid demand using a four compartment electrolytic cell.

Colon, Guillermo↗

Development of Plant Gene Vectors for Tissue-Specific Expression Using GFP as a Reporter Gene

Reporter genes are widely employed in plant molecular biology research to analyze gene expression and to identify promoters. Gus (UidA) is currently the most popular reporter gene but its detection requires a destructive assay. The use of jellyfish green fluorescent protein (GFP) gene from Aequorea Victoria holds promise for noninvasive detection of in vivo gene expression. To study how various plant promoters are expressed in sweet potato (Ipomoea batatas), we are transcriptionally fusing the intron-modified (mGFP) or synthetic (modified for codon-usage) GFP coding regions to these promoters: double cauliflower mosaic virus 35S (CaMV 35S) with AMV translational enhancer, ubiquitin7-intron-ubiquitin coding region (ubi7-intron-UQ) and sporaminA. A few of these vectors have been constructed and introduced into E. coli DH5a and Agrobacterium tumefaciens EHA105. Transient expression studies are underway using protoplast-electroporation and particle bombardment of leaf tissues.

Jackson, Jacquelyn↗

Effects of Bioreactor Retention Time on Aerobic Microbial Decomposition of CELSS Crop Residues

The focus of resource recovery research at the KSC-CELSS Breadboard Project has been the evaluation of microbiologically mediated biodegradation of crop residues by manipulation of bioreactor process and environmental variables. We will present results from over 3 years of studies that used laboratory- and breadboard-scale (8 and 120 L working volumes, respectively) aerobic, fed-batch, continuous stirred tank reactors (CSTR) for recovery of carbon and minerals from breadboard grown wheat and white potato residues. The paper will focus on the effects of a key process variable, bioreactor retention time, on response variables indicative of bioreactor performance. The goal is to determine the shortest retention time that is feasible for processing CELSS crop residues, thereby reducing bioreactor volume and weight requirements. Pushing the lower limits of bioreactor retention times will provide useful data for engineers who need to compare biological and physicochemical components. Bioreactor retention times were manipulated to range between 0.25 and 48 days. Results indicate that increases in retention time lead to a 4-fold increase in crop residue biodegradation, as measured by both dry weight losses and CO2 production. A similar overall trend was also observed for crop residue fiber (cellulose and hemicellulose), with a noticeable jump in cellulose degradation between the 5.3 day and 10.7 day retention times. Water-soluble organic compounds (measured as soluble TOC) were appreciably reduced by more than 4-fold at all retention times tested. Results from a study of even shorter retention times (down to 0.25 days), in progress, will also be presented.

Strayer, R. F.↗

Experiment 8: Environmental Conditions in the ASTROCULTURE(trademark) Plant Chamber During the USML-2 Mission

Conducting plant research to assess the impact of microgravity on plant growth and development requires a plant chamber that has the capability to control other environmental parameters involved in plant growth and development. The environmental control in a space-based plant chamber must be equivalent to that available in such facilities used for terrestrial plant research. Additionally, plants are very sensitive to a number of atmospheric gaseous materials. Thus, the atmosphere of a plant chamber must be isolated from the space vehicle atmosphere, and the plant growth unit should have the capability to remove any such deleterious materials that may impact plant growth and development. The Wisconsin Center for Space Automation and Robotics (WCSAR), University of Wisconsin-Madison, has developed a totally enclosed controlled environment plant growth unit. The flight unit was used to support the ASTROCULTURE(TM) experiment conducted during the USML-2 mission. The experiment had two major objectives: 1) Provide further validation of the flight unit to control the experiment-defined environmental parameters in the plant chamber, and 2) support a plant experiment to assess the capability of potato plant material to produce tubers in microgravity. This paper describes the temperature, humidity, and carbon dioxide conditions of the plant chamber during the mission, from launch to landing. Another paper will present the plant response data.

Bula, R. J.↗

Space Age Spuds

American Ag-Tech International, Ltd. developed a system called Quantum Tubers through the Wisconsin Center for Space Automation and Robotics (a NASA-sponsored Commercial Space Center). Using computerization and technologies originally intended for growing plants in space, the company developed a growth chamber that accelerates plant growth and is free of plant pathogens. The chamber is used to grow minitubers, which serve as nuclear seed stock for potatoes. Using lighting technology, temperature and humidity controls, and automation technology, the minituber can be generated in one closed facility with out much labor handling. This means they can be grown year round in extreme environments. The system eliminates the need for multiple generations of seed and eliminates exposure to pathogens, disease and pests. The Quantum Tubers system can produce 10-20 million tubers throughout the year, about equal to the world's supply of this generation seed stock.

Source record↗

Bioregenerative Life Support Systems Test Complex (Bio-Plex) Food Processing System: A Dual System

A Bioregenerative Life Support Test Complex, BIO-Plex, is currently being constructed at the Johnson Space Center (JSC) in Houston, TX. This facility will attempt to answer the questions involved in developing a lunar or planetary base. The Food Processing System (FPS) of the BIO-Plex is responsible for supplying food to the crew in coordination with the chosen mission scenario. Long duration space missions require development of both a Transit Food System and of a Lunar or Planetary Food System. These two systems are intrinsically different since the first one will be utilized in the transit vehicle in microgravity conditions with mostly resupplied foods, while the second will be used in conditions of partial gravity (hypogravity) to process foods from crops grown in the facility. The Transit Food System will consist of prepackaged food of extended shelf life. It will be supplemented with salad crops that will be consumed fresh. Microgravity imposes significant limitation on the ability to handle food and allows only for minimal processing. The challenge is to develop food systems similar to the International Space Station or Shuttle Food Systems but with a shelf life of 3 - 5 years. The Lunar or Planetary Food System will allow for food processing of crops due to the presence of some gravitational force (1/6 to 1/3 that of Earth). Crops such as wheat, soybean, rice, potato, peanut, and salad crops, will be processed to final products to provide a nutritious and acceptable diet for the crew. Not only are constraints imposed on the FPS from the crops (e.g., crop variation, availability, storage and shelf-life) but also significant requirements are present for the crew meals (e.g., RDA, high quality, safety, variety). The FPS becomes a fulcrum creating the right connection from crops to crew meals while dealing with issues of integration within a closed self-regenerative system (e.g., safe processing, waste production, volumes, air contaminations, water usage, etc.). Options for the first test, for duration of 120 days, currently scheduled for late 2003 are outlined.

Perchonok, Michele↗