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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 127 records · Page 7

Three-dimensional thermal-structural analysis of a swept cowl leading edge subjected to skewed shock-shock interference heating

A three-dimensional flux-based thermal analysis method has been developed and its capability is demonstrated by predicting the transient nonlinear temperature response of a swept cowl leading edge subjected to intense three-dimensional aerodynamic heating. The predicted temperature response from the transient thermal analysis is used in a linear elastic structural analysis to determine thermal stresses. Predicted thermal stresses are compared with those obtained from a two-dimensional analysis which represents conditions along the chord where maximum heating occurs. Results indicate a need for a three-dimensional analysis to predict accurately the leading edge thermal stress response.

Polesky, Sandra P.↗

Analysis of the flow in a 1-MJ electric-arc shock tunnel

In the electric-arc-heated shock tunnel, the facility performance over a range of shock Mach numbers from 7 to 19 was evaluated. The efficiency of the arc-heated driver is deduced using an improved form of the shock tube equation. A theoretical and experimental analysis is made of the tailored-interface condition. The free stream properties in the test section, with nitrogen as the test gas, are evaluated using a method based on stagnation point, heat transfer measurements.

Reller, J. O., Jr.↗

Shock interference peak heating measurements using phase change coatings

The phase change coating technique is used to obtain peak heating measurements in shock interference flow regions with high surface shear and heating. This technique provides heat transfer coefficients which are determined by measuring the time for a point on the surface to reach the phase change temperature of the thin fusible coating. Measurements were conducted on a 5.08-cm diameter hemisphere-cylinder made of silica based epoxy at Mach 6 for free stream Reynolds numbers of 3.3 to 25.6 million per meter. A sketch of the shock interference pattern produced by a flat plate shock generator is included. Heating data obtained on a 5.08-cm diameter stainless steel hemispherical model instrumented with thermocouples is presented for the purpose of comparing the phase change technique with the thermocouple-calorimeter technique.

Keyes, J. W.↗

Inhibition of HSP70 and a Collagen-Specific Molecular Chaperone (HSP47) Expression in Rat Osteoblasts by Microgravity

Rat osteoblasts were cultured aboard a space shuttle for 4 or 5 days. Cells were exposed to 1alpha, 25 dihydroxyvitamin D(3) during the last 20 h and then solubilized by guanidine solution. The mRNA levels for molecular chaperones were analyzed by semi-quantitative RT-PCR. ELISA was used to quantify TGF-beta1 in the conditioned medium. The HSP70 mRNA levels in the flight cultures were almost completely suppressed, as compared to the ground (1 x g) controls. The inducible HSP70 is known as the major heat shock protein that prevents stress-induced apoptosis. The mean mRNA levels for the constitutive HSC73 in the flight cultures were reduced to 69%, approximately 60% of the ground controls. HSC73 is reported to prevent the pathological state that is induced by disruption of microtubule network. The mean HSP47 mRNA levels in the flight cultures were decreased to 50% and 19% of the ground controls on the 4th and 5th days. Concomitantly, the concentration of TGF-beta1 in the conditioned medium of the flight cultures was reduced to 37% and 19% of the ground controls on the 4th and 5th days. HSP47 is the collagen-specific molecular chaperone that controls collagen processing and quality and is regulated by TGF-beta1. Microgravity differentially modulated the expression of molecular chaperones in osteoblasts, which might be involved in induction and/or prevention of osteopenia in space.

Animals↗

Chromospheric heating by acoustic shock waves

Work by Anderson & Athay (1989) suggests that the mechanical energy required to heat the quiet solar chromosphere might be due to the dissipation of weak acoustic shocks. The calculations reported here demonstrate that a simple picture of chromospheric shock heating by acoustic waves propagating upward through a model solar atmosphere, free of both magnetic fields and local inhomogeneities, cannot reproduce their chromospheric model. The primary reason is the tendency for vertically propagating acoustic waves in the range of allowed periods to dissipate too low in the atmosphere, providing insufficient residual energy for the middle chromosphere. The effect of diverging magnetic fields and the corresponding expanding acoustic wavefronts on the mechanical dissipation length is then discussed as a means of preserving a quasi-acoustic heating hypothesis. It is argued that this effect, in a canopy that overlies the low chromosphere, might preserve the acoustic shock hypothesis consistent with the chromospheric radiation losses computed by Anderson & Athay.

Jordan, Stuart D.↗

Distinct transcripts are recognized by sense and antisense riboprobes for a member of the murine HSP70 gene family, HSP70.2, in various reproductive tissues

The expression of hsp70.2, an hsp70 gene family member, originally characterized by its high levels of expression in germ cells in the adult mouse testis, was detected in several other reproductive tissues, including epididymis, prostate, and seminal vesicles, as well as in extraembryonic tissues of mid-gestation fetuses. In addition, hybridization with RNA probes transcribed in the sense orientation surprisingly indicated the presence of slightly larger "antisense" transcripts in several tissues. The levels of antisense transcripts varied among the tissues, with the highest signal detected in the prostate and no signal being detectable in the testis. Consistent with these results, in situ hybridization analysis clearly localized the sense-orientation transcripts to pachytene spermatocytes, while no antisense-orientation transcripts were observed in adjacent sections of the same tubules. Our findings have thus shown that although hsp70.2 was expressed abundantly and in a highly stage-specific manner in the male germ line, it was also expressed in other murine tissues. Furthermore, we have made the surprising observation of antisense transcription of the hsp70.2 gene in several mouse tissues, revealing another level of complexity in the regulation and function of heat shock proteins.

Non-NASA Center↗

Sense and antisense transcripts of the developmentally regulated murine hsp70.2 gene are expressed in distinct and only partially overlapping areas in the adult brain

We have examined the spatial pattern of expression of a member of the hsp70 gene family, hsp70.2, in the mouse central nervous system. Surprisingly, RNA blot analysis and in situ hybridization revealed abundant expression of an 'antisense' hsp70.2 transcript in several areas of adult mouse brain. Two different transcripts recognized by sense and antisense riboprobes for the hsp70.2 gene were expressed in distinct and only partially overlapping neuronal populations. RNA blot analysis revealed low levels of the 2.7 kb transcript of hsp70.2 in several areas of the brain, with highest signal in the hippocampus. Abundant expression of a slightly larger (approximately 2.8 kb) 'antisense' transcript was detected in several brain regions, notably in the brainstem, cerebellum, mesencephalic tectum, thalamus, cortex, and hippocampus. In situ hybridization revealed that the sense and antisense transcripts were both predominantly neuronal and localized to the same cell types in the granular layer of the cerebellum, trapezoid nucleus of the superior olivary complex, locus coeruleus and hippocampus. The hsp70.2 antisense transcripts were particularly abundant in the frontal cortex, dentate gyrus, subthalamic nucleus, zona incerta, superior and inferior colliculi, central gray, brainstem, and cerebellar Purkinje cells. Our findings have revealed a distinct cellular and spatial localization of both sense and antisense transcripts, demonstrating a new level of complexity in the function of the heat shock genes.

NASA Discipline Developmental Biology↗

Extremophiles in astrobiology: per Ardua ad Astra

As we consider the possibilities of finding life on other planets, it behooves us to evaluate what we know about the limits for life on planet Earth. In our continued exploration of Earth, we are finding microbes in a variety of unexpected habitats. In geothermal hot springs, we have discovered organisms thriving at temperatures near the boiling point of water and at pH values down to 0.5; in the deepest parts of the oceans, those that grow optimally at pressures above 1000 bars and die at pressures below 500 bars; and at the poles, those that grow below the freezing point of water and die at temperatures above 10 degrees C. All of these organisms are living proof that the biochemical "machinery" of life can be adapted to conditions that, from our anthropocentric perspective, appear to be extreme. By studying the molecular adaptations of extremophiles, we begin to identify the critical cellular components that expand the envelope for life. As an example, I will discuss what we have learned about the role of the proteins we call "heat shock proteins" in pushing the upper temperature limit of life and how our studies have provided a new perspective on the function of these proteins.

Chaperonin 60/physiology↗

Chaos and ion heating in a slow shock

An ion heating mechanism is proposed of slow shocks, which is associated with the chaotic motion of particles in the downstream wave field. For a coherent electromagnetic wave propagating along the downstream magnetic field, corresponding to switch-off shocks, the particle motions are not chaotic. For an oblique wave, the interaction between the particles and the wave field may lead to chaotic particle motions. Such particles may be greatly thermalized within one wavelength after they are incident into the downstream wave field. The results can be used to explain the existence of the critical intermediate Mach number observed in the hybrid simulations.

Lin, Y.↗

Observations of the extinction and excitation of the molecular hydrogen emission in Orion

Observations have been made of two quadrupole purely rotational transitions of molecular hydrogen, the 0-0 S(7) and S(5) lines, and of several previously unobserved vibration-rotation hydrogen lines in the Orion molecular cloud. The line ratios indicate a 2.1-micron extinction of about 2 mag. A comparison of the populations of three vibrational levels shows a distribution of excitation temperatures between 1000 and 3000 K. These values are consistent with the theory that hydrogen is thermally excited in shock-heated gas which cools after passage of the shock front. The total luminosity of the hot molecular hydrogen is 200 plus or minus 80 solar luminosity, and the mass of the emitting gas is of the order 0.05 solar mass. The mass and cooling times of the hot hydrogen indicate that at least 10 solar mass of the material has been heated over the lifetime of the outflow.

Beckwith, S.↗

Shuttle ascent and shock impingement aerodynamic heating studies

The collection and analysis of aerodynamic heating data obtained from shock impingement experimental investigation were completed. The data were categorized into four interference areas; fin leading edge, wing/fuselage fin/plate corners, and space shuttle configurations. The effects of shock impingement were found to increase the heating rates 10 to 40 times the undisturbed values. A test program was completed at NASA/Langley Research Center to investigate the magnitudes and surface patterns of the mated shock interference flowfield. A 0.0065 scale thin-skin model of the MDAC 256-20 space shuttle booster mated with a Stycast model of the MDAC Internal tank orbiter was tested in the 20-inch M=6 tunnel, the 31-inch M=10 tunnel, and the 48-inch Unitary Plan Tunnel. The gap region of the ascent configuration was the principal area of interest where both thermocouple and phase-change paint data were obtained. Pressure and heat transfer distributions data on the leeward surface of a 75-degree sweep slab delta wing are presented. The effects of surface roughness on boundary layer transition and aerodynamic heating were investigated.

Lanning, W. D.↗